TH35885B - Recombinant preparation (Recombinant) Factor VIII in Protein-Free Culture Diets - Google Patents

Abstract

DC60 (24/03/41) Recombinant (recombinant) Factor VIII that can be produced in relatively large quantities. Continuously extracted from mammalian cells without the presence of any animal-derived proteins such as albumin by cell culture in protein-free cultured medium supplemented with poly. Old copolymers, which are often present in trace metals very small quantities) such as copper, in a very favorable manner, culture food It will cover Known polyols as Pluronic F-68, copper sulphate, ferrous sulphate/EDTA complexes, and tracemetal salts such as Manganese, Molybdenum, Silicon, Lithium, and Chromium Recombinant (recomvinant) Factor VIII that can be produced in relatively large quantities. Continuously extracted from mammalian cells without the presence of any animal-derived proteins such as albumin by cell culture in protein-free cultured medium supplemented with poly. Old copolymers, which are often present in trace metals very small quantities) such as copper, in a very favorable manner, culture food It will cover Known polyols as Pluronic F-68, copper sulphate, ferrous sulphate/EDTA complexes, and tracemetal salts such as manganese, molybdenum, silicon, lithium, and chrome

Claims (11)

1. The method for producing recombinant factor VIII from mammalian cells genetically modified for this involves culturing the host cells (mammalian cells) in a plasma-derived protein-free culture medium supplemented with polyoles and copper ions. 2. Method of Claim 1, where the polyole is Pluronic F-68 and is present in culture media at concentrations ranging from 0.1 to 0.3% by weight. 3. Method of Claim 1, where the culture medium includes copper sulfate in amounts ranging from 50 to 800 nanomoles. 4. Method of Claim 1, where the culture medium is supplemented with 3 nanomoles of MnSO4. 5. Method of Claim 1, where in the culture medium supplemented with [NH4]6Mo7O24,4H2O at 3 nanomoles. 6. Method of Claim 1, where the culture medium is supplemented with 1.5 nanomoles of CrK(SO4)7,4H2O. 7. Method of Claim 1, where the culture medium is supplemented with LiCl at 236 nanomoles. 8. Method of Claim 1, where the host cell, which is a mammalian cell, was selected from a group consisting of baby hamster kidney cells, human embryonic kidney cells, Chinese hamster ovary cells. 9. Cell culture medium for the production of recombinant factor VIII, consisting of a basic culture medium free of plasma-derived proteins and including pollol and copper ions. 10. The culture medium of claim 9 shall consist of at least one tace metal (a metallic element present in very small amounts) selected from the group of manganese, molybdenum, silicon, chromium, and lithium. 11. The culture medium for claim 10, supplemented with insulin.