TH40672A - New selection markers - Google Patents

Abstract

DC60 (23/06/41) This invention concerns the use of cyanamide hydratase as a selection marker in plant transformation. Cyanamide acts as a herbicide, and plants transformed with the gene for producing cyanamide hydratase can convert cyanamide into urea. This allows for the selection of transformed plants, which can survive under cyanamide-stressed conditions. This invention concerns the use of cyanamide hydratase as a selection marker in plant transformation. Cyanamide acts as a herbicide, and plants transformed with the gene for producing cyanamide hydratase can convert cyanamide into urea. This allows for the selection of transformed plants, which can survive under cyanamide-stressed conditions.

Claims (11)

1. Using cyanamide hydratase as a marker. 1. Using cyanamide hydratase as a marker for selecting beetles. 2. Using cyanamide hydratase as a selection marker in transgernier experiments for plant form selection. 3. The use of claim 2, which states that the plant is transformed by a specific sequence of nucleotides for the production of cyanamide hydratase, and nucleotides for cyanamide hydratase. 4. The use of claim 3, which characterizes the use of the ninglotide sequence as shown in SEQ ID NO:1. 5. The process for selecting transformed plants consists of (a) creating a vector containing a nucleotide sequence for constructing cyanamide hydratase and the gene of interest; (b) transforming the plant, plant parts, or plant cells using the said vector; and (c) culturing the transformed plant in a culture medium containing cyanamide. 6. The method stipulated in claim 5, which specifically identifies the nucleotide sequence of SEQ ID NO: 1, is used. 7. The use of cyanamide for the selection of plants transformed by vectors containing cleotide sequences for the production of cyanamide hydratase. 8. An express cassette containing the nucleotide sequence for synthesizing cyanamide hydratase. 9. A vector containing an expression cassette pursuant to claim number 8. 10. A host cell containing the vectors as per claim number 9. 11. The host cell according to claim 8 is Agrobacterium (11 claims, illustration - picture).