TH60266A - Methods for the production of nucleotides by fermentation. - Google Patents

Abstract

DC60 (17/09/44) Nucleoside 5'-Phosphate Ester is produced by culturing bacteria of the Escherichia family capable of producing 5'-F nucleosides. Phosphate ester, where the ushA gene and the aphA gene are broken down, causing them to not function normally in the medium to produce and accumulate 5'-phosphate ester nucleosides in the medium. And collection of 5'-phosphate ester nucleosides from the medium; 5'-phosphate ester nucleosides were produced by bacterial cultures of The Escherichia family capable of producing 5'-phosphate ester nucleosides, where the ushA and aphA genes are broken down, causing them to not function normally in the medium to produce and accumulate new nucleosides. Cleoside 5'-phosphate ester in medium and collection of 5'-phosphate ester from that medium.

Claims (8)

1. The method for producing 5'-phosphate nucleoside esters involves the following steps: culturing Escherichia bacteria capable of producing 5'-phosphate esters, in which the ushA and aphA genes are non-functional, in a medium to produce and accumulate 5'-phosphate esters within the medium, and finally collecting the 5'-phosphate esters from that medium. 2. A method for the production of nucleoside 5'-phosphate esters according to claim 1, where mutations are introduced into the ushA gene and the aphA gene, or these genes are broken down, rendering them non-functional. 3. Method for the production of nucleoside 5'-phosphate esters under claim 1 or 2, whereby the nucleoside 5'-phosphate ester is selected from a group containing 5'-inosinic acid or 5'-guanilic acid. 4. Bacteria of the Escherichia family capable of producing 5'-phosphate nucleoside esters, in which the ushA and aphA genes are cleavaged. 5. Bacteria of the Escherichia genus according to claim 4, where the 5'-phosphate ester nucleoside is selected from the group containing 5'-inosinic acid or 5'-guanilic acid. 6. A method for discovering 5'-nucleotidase genes that yield 5'-phosphate ester nucleoside accumulation, consisting of the steps of culturing the parent strain of the microbial plant and its derivative strains, where known 5'-nucleotidase is removed in the least common medium containing the first 5'-phosphate ester nucleoside as the single carbon source and the least common medium containing the second 5'-phosphate ester nucleoside as the single carbon source, to examine the gene expression characteristics in the parent and derivative strains; calculating the yield ratio of the expression levels of each gene in the parent and derivative strains when cultured in the least common medium containing the first 5'-phosphate ester nucleoside as the single carbon source and the least common medium containing the second 5'-phosphate ester nucleoside as the single carbon source; and selecting one or more genes showing a higher yield. 7. Method for discovering the 5'-nucleotidase gene according to claim 6, where the first and second 5'-phosphate ester nucleosides are made of 5'-inosinic acid or 5'-guanilic acid. 8. A method for discovering 5'-nucleotidase genes pursuant to claim 6 or 7, comprising a step for selecting genes capable of transcribing the desired signal sequence for protein conversion to periplasm from the selected genes.