TH67946A - A new enzyme that catalyzes peptide formation and the microorganisms that produce it. And how this microorganism is produced - Google Patents

Abstract

DC60 (02/10/46) This invention involves a new enzyme. That can easily form peptides, with high yield and low cost. Without the need for complicated processes In detail is Is a type of enzyme New that catalyzes peptide production from Carboxy assembly And amine composition And also involved in microorganisms that produce this enzyme As well as how the enzyme or microorganisms can be used to produce low-cost dipeptides, the inventor has discovered a new enzyme derived from bacteria in genus. MPDB, which can Production of peptides efficiently, as well as finding a way to make such peptides cheap and easy, the invention involved a new enzyme. That can easily form peptides, with high yield and low cost. Without the need for a complicated process. In detail is Is a type of enzyme New that catalyzes peptide production from Carboxy assembly And amine composition And also involved in microorganisms that produce this enzyme As well as how the enzyme or microorganisms can be used to produce low-cost dipeptides, the inventor has discovered a new enzyme derived from bacteria in genus. MPDB, which can Efficiently produced peptides, and found a method for making such peptides cheap and easy.

Claims (11)

1. Enzymes derived from bacteria of the genus Empedobactor or Sphingobactenium that are capable of synthesizing peptides containing both carboxymethyl and amine components. 2. Enzymes capable of synthesizing peptides from carboxylic and amine components under the conditions of dipeptide synthesis as in (i) - (iv) below, and with a L-alany-L-glutamine synthesis rate of 0.03 mM/min or higher. i) The carboxylic component is L-alaninemethylester hydrochloride (100 mM). ii) The amine component is L-glutamine (200 mM). iii) pH equals 9.0. iv) The amount of enzyme added is not more than 0.61 mg protein/ml. 3. An enzyme under claim 1 or 2 containing a carboxylic acid that can use an amino acid ester or amino acid amide as a substrate. 4. Any enzyme in claims 1-3 that contains an amine component capable of utilizing an amino acid, a protected C amino acid, or an amine substrate. 5. Which of the enzymes in claims 1-4 has the ability to synthesize peptides at pH 6.5-10.5? 6. Which of the enzymes in claims 1-5 has the ability to synthesize peptides at temperatures of 0-60 degrees Celsius? 7. One of the enzymes in points 1-6 is not inhibited by PMSF (phenylmethylsulfonylfluoride), an inhibitor of serine protease, but is inhibited by p-nitrophenyl-p''-guanidebenzoate. 8. One of the enzymes in claims 1-7 has a molecular weight of approximately 75 kDa when subjected to SDS-PAGE and approximately 150 kDa when subjected to gel filtration. 9. Microorganisms that produce one of the enzymes mentioned in claims 1-8. 10. The microorganisms mentioned in item 9, namely Empedobacter brevis FERM BP-8113 strain or Sphingobacterium sp. FERM BP-8124 strain. 11. Methods for producing dipeptides using enzymes or enzyme mixtures as described in any of the claims 1-8 for the synthesis of dipeptides from carboxymethyl and amine components.