TH70237A - L-lactic acid production process - Google Patents

Abstract

DC60 (29/04/46) Lactic acid production from glucose or hydrolysate from enzymatic hydrolysis of cassava starch. The production process is conducted using both continuous production with cell recycling and two-stage continuous production with cell recycling. Nutrients (glucose or cassava starch hydrolysate) are supplied under controlled steady-state conditions suitable for one- or two-stage continuous fermentation with cell recycling.

Claims (2)

Revised 11/10/2018. Claim 1. Continuous L-lactic acid production process with cell recycling system at a dilution rate (D) of 0.96 per hour, a bleed ratio (B) of 0.25, and a glucose concentration in the culture medium of 23.03 g/L. The complete culture time is 12 hours and the continuous culture time is 69 hours. 1. Continuous lactic acid production with a cell recycling process was performed at a dilution rate (D) of 0.96 per hour, a bleed ratio (B) of 0.25, and a glucose concentration in the culture medium of 23.03 g/L. The total culture time was 12 hours, and the continuous culture time was 69 hours. 2. Two-stage continuous lactic acid production with a cell recycling system, using dilution ratios D1 = 0.08 and D2 = 0.30 per hour, a direct separation ratio from the first fermenter B1 = 0.5, and glucose concentrations in the added culture medium of 29.93 or 39.51 g/L, with complete culture durations of 12-14 hours and continuous culture durations of 34-36 hours.