TH71899B - Methods for the production of target substances by fermentation. - Google Patents

Abstract

DC60 (29/08/46) in methods for the production of microbial target substances, which consist of cultured Gamma-protease inhibitors in the medium for the production and accumulation of the target substance in the medium or cell and for the collection of that target substance. Strains in which ArcA proteins do not function normally in cells are used by methods, for example by rupturing the arcA gene on a chromosome.

Claims (9)

Claims (all) which will not appear on the advertisement page :Edit 1 Oct. '18 Claims 1. That includes the substitution, subtraction, addition or addition of 2 to 20 amino acids and improving the ability of (Formula)-proteobacterium to provide target metabolites when the production of proteins is reduced or depleted in (formula)-proteobacterium compared to the original or non-proteobacterium species. adapt ++++++++++++++++++++++++++++++++++++++++ ------01/12/ 2560------(OCR) Page 1 of 2 pages Claims 1. That includes the substitution, subtraction, addition or addition of 1 to 20 amino acids and improvements in the ability of (Recipe)-Proteo Bacteria to target metabolites when protein production is reduced or depleted in (formula)- proteobacterium compared to the original or unmodified species. 2. Claim Method 1, where the ArcA protein as defined in (B) is a protein with 90% or more of the same as the amino acid sequence of SEQ ID NO:32. and improvement ability (formula)-proteobacterium to produce the target substance When the production of protein is reduced or depleted in (Formula)-proteobacterium compared to the original or unmodified species. 3. 1. Or a tracer that can be produced from a nucleo-arranging sequence. tide under strict conditions and transcription for proteins that improve the ability of (Recipe)-Proteo Bacteria to produce target substances when protein production is reduced. or eliminated in (Formula)-Proteo, page 2 of number 2, Bacteria compared to the original or unmodified species. where such strict conditions are 1 X SSC, 0.1% SDS at 60°C. Bacteria belonging to the genus Escherichia or Pantoea ------------ 1. Gamma-Proteobacterium that are capable of producing target metabolites and modified so that ArcA proteins do not function normally 1., which includes the substitution, subtraction, addition or addition of one or more amino acids and thus ability to produce target substances Means improved when the protein was not functioning normally in gamma-proteobacterium. in comparison with the case where proteins function normally 1. and improves the ability to produce target metabolites when proteins are not functioning normally in gamma-proteobacterium Compared to the case when proteins function normally. 4. Gamma-proteobacterium according to claim 1, where the normally functional ArcA protein is an amino acid arrangement of 32 arrangement, including substitution, substitution. ejection, addition, or addition of 2 to 20 amino acids and improves the ability to produce target substances when Proteins are not functioning normally in gamma-proteobacteria. compared to cases where proteins function normally 5. Gamma-proteobacterium according to any of Claims 1 to 4, where the ArcA protein does not function normally by means of cleavage of the arcA gene on the chromosome. 6. 1.Or assays that can be produced from that nucleotide under strict conditions and decoded for proteins that improve their ability to produce target metabolites when proteins do not. normal duties in gamma-proteobacterium Compared to the case when proteins function normally. 7. Gamma- any proteobacterium of claims 1 to 6, where bacteria belong to the Escherichia family. 8. Gamma- any proteobacterium of claims 1 to 7, where the target substance is an L-amino acid. 9. Gamma-proteobacterium according to claim 8, where L-amino acids are selected from a group consisting of L-lysine, L-glutamic acid. and L-arginine 1 0. Methods for producing target substances that includes culture gamma-proteo Bacteria pursuant to any of Claims 1 to 9 in a medium for the production and accumulation of the target substance in the medium or cell and the collection of the target substance from that medium or cell.