TH9821A3 - Preparation process for Inthanin flower extract with high phenolic flavonoids and anthocyanin. And has an inhibitory effect on collagen breakdown enzymes - Google Patents

Abstract

DC60 (June 27, 2014) This report describes a method for preparing a high-phenolic, flavonoid, and anthocyanin-rich extract of Lagerstroemia speciosa (Indian crape myrtle) flowers with collagen-degrading enzyme inhibitory activity. The extraction process involved separating high concentrations of phenolic compounds, flavonoids, and anthocyanins from Lagerstroemia speciosa petals using organic solvent fermentation mixed with water. The extract was then dried at low temperatures using a combination of refrigerated and ultra-cold evaporation under vacuum conditions. The resulting extract is a dry, purplish-brown powder that is readily soluble in both water and alcohol. A previously unreported activity was discovered: the inhibition of matrix metalloproteinase enzymes that degrade collagen. This makes the extract suitable for use as a key ingredient in skincare products or for applications involving collagen breakdown disorders. This invention not only supports the utilization of Thai herbs but also adds value to Thai flowers, which are not only used as ornamental plants but also offer potential for medicinal use in skincare product development. This method for preparing a high-phenolic, flavonoid, and anthocyanin-rich extract of Lagerstroemia speciosa (Indian crape myrtle) flowers, exhibiting collagen-degrading enzyme inhibitory activity, involves extracting these important phenolic compounds from the petals using an organic solvent fermentation method mixed with water. The extract is then starched at low temperatures and subjected to high-temperature evaporation and vacuum drying. The resulting extract is a dry, purplish-brown powder that is readily soluble in both water and alcohol. A previously unreported activity was discovered: the inhibition of matrix metalloproteinase enzymes that degrade collagen. This makes the extract suitable for use as a key ingredient in skincare products or for applications involving collagen degradation disorders. This invention not only supports the utilization of Thai herbs but also adds value to Thai flowers, which are not only used as ornamental plants but also offer potential medicinal uses in skincare product development.

Claims (1)

1. Method for preparing Inthanin flower extract with high phenolic flavonoids and anthocyanin and inhibiting enzyme for collagen breakdown. The steps are as follows: a. Only the reddish-purple petals of the Inthanin flower are dried at 45 degrees Celsius for 2 days and ground them with a dry grinding machine until particle size is 200-250 microns. Inthanin flower petals dried from Section A. are soaked in a mixture containing distilled water and alcohol (1: 1) by soaking in a ratio of 1:18 shaken together at a temperature of 20-25 degrees Celsius and in a container. Opaque until the Inthanin flower powder swells fully. Then continue to ferment by stirring periodically with a stick stirrer. Magnet times for 20 minutes, 15-20 times a day, for 5 days. C. Take the fermented part from item B. Subsequently, pass the No. 1 filter paper. Dip the extracts that are filtered through the filter paper from Article C. to evaporate. Remove the alcohol using the machine. Evaporate, rotary dry at 45 ° C for 6-10 hours until the extracts become viscous, remaining 1 part of the total solution. E. Dry the parts from section D and dry with a cold dryer at temperature. -50 degrees Celsius for 1-2 days until the extract was completely dried purple powder which gave% yield of 22 which This has good solubility in both water and alcohol.