TR199901840T2 - DNA'nin cross-flow-filtrasyonu ile temizlenmesi. - Google Patents

DNA'nin cross-flow-filtrasyonu ile temizlenmesi.

Info

Publication number
TR199901840T2
TR199901840T2 TR1999/01840T TR9901840T TR199901840T2 TR 199901840 T2 TR199901840 T2 TR 199901840T2 TR 1999/01840 T TR1999/01840 T TR 1999/01840T TR 9901840 T TR9901840 T TR 9901840T TR 199901840 T2 TR199901840 T2 TR 199901840T2
Authority
TR
Turkey
Prior art keywords
nucleic acid
cross
flow
solution
cleaning
Prior art date
Application number
TR1999/01840T
Other languages
English (en)
Inventor
Kuhne Wolfgang
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of TR199901840T2 publication Critical patent/TR199901840T2/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)

Abstract

Bulus, nükleikasitlerin bir çözeltide temizlenmesine ve/veya konsantre edilmesine mahsus bir usul ile ilgilidir, burada nükleikasitleri içeren çözelti, nükleikasit-molekülleri zarlardan uzak tutulacak ve düsük moleküler agirlikli maddeler, zarlardan geçebilecek ve/veya zarda adsorbe edilecek biçimde ve temizlenmis ve/veya konsantre edilmis bir nükleikasit-çözeltisi elde edilecek sekilde tegetsel olarak bir veya birçok yari geçirgen zarin yanindan geçirilir. Ayni sekilde endotoksinlerin bir nükleikasit preperasyonundan ayrilmasindada hareket edilir. Bulus ayrica nükleikasitlerin bir çözeltide temizlenmesine ve/veya konsantre edilmesine ve ayni zamanda endotoksinlerin bir nükleikasit-preperasyonundan ayrilmasina mahsus bir Cross-Flow-Filtrasyon düzeneginin kullanimi ilede ilgilidir.
TR1999/01840T 1997-01-10 1998-01-09 DNA'nin cross-flow-filtrasyonu ile temizlenmesi. TR199901840T2 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97100331A EP0853123A1 (de) 1997-01-10 1997-01-10 Reinigung von DNA durch Cross-Flow-Filtration

Publications (1)

Publication Number Publication Date
TR199901840T2 true TR199901840T2 (tr) 2000-02-21

Family

ID=8226362

Family Applications (1)

Application Number Title Priority Date Filing Date
TR1999/01840T TR199901840T2 (tr) 1997-01-10 1998-01-09 DNA'nin cross-flow-filtrasyonu ile temizlenmesi.

Country Status (8)

Country Link
EP (2) EP0853123A1 (tr)
JP (1) JP2000513580A (tr)
KR (1) KR100322398B1 (tr)
CN (1) CN1243543A (tr)
BR (1) BR9807062A (tr)
CA (1) CA2277165C (tr)
TR (1) TR199901840T2 (tr)
WO (1) WO1998030685A2 (tr)

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US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids
DE19900681B4 (de) 1999-01-04 2007-04-12 Sartorius Ag Verwendung von Anionaustauschermembranen zur Entfernung von Endotoxinen aus Nukleinsäure-haltigen Lösungen
KR100669305B1 (ko) * 1999-07-23 2007-01-17 제넨테크, 인크. Ｒｎａ 분해효소 및 유기용매의 작용없이 접선 흐름여과를 이용한 플라스미드 ｄｎａ 정제법
US6284117B1 (en) * 1999-12-22 2001-09-04 Nanogen, Inc. Apparatus and method for removing small molecules and ions from low volume biological samples
DE10006591B4 (de) * 2000-02-11 2007-03-29 Eppendorf Ag Verfahren zur Aufreinigung von Nukleinsäuren
DE10022258A1 (de) * 2000-05-08 2001-11-15 Bayer Ag Reinigung von Proteineinschlusskörpern durch Querstrom-Mikrofiltration
WO2001094573A2 (en) * 2000-06-02 2001-12-13 Pall Corporation Processing of plasmid-containing fluids
KR100356738B1 (ko) * 2000-09-07 2002-10-18 주식회사 삼양제넥스 염기성 단백질로부터 엔도톡신을 제거하는 방법
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
JP2005538929A (ja) 2002-01-16 2005-12-22 ダイナル バイオテック エイエスエイ 単一サンプルからの核酸及びタンパク質の単離方法
CA2498518A1 (en) 2002-09-13 2004-03-25 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
AU2003302418A1 (en) * 2002-11-28 2004-06-18 Arkray Inc. Method and apparatus for concentration and purification of nucleic acid
EP1628749B1 (en) 2003-05-30 2019-07-31 VGXI, Inc. Devices and methods for biomaterial production
US8075780B2 (en) * 2003-11-24 2011-12-13 Millipore Corporation Purification and concentration of synthetic biological molecules
JP5795255B2 (ja) * 2008-07-18 2015-10-14 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． 微小流体ｄｎａ試料調製のための方法およびシステム
HUE042177T2 (hu) 2009-12-01 2019-06-28 Translate Bio Inc Szteroidszármazék mRNS szállítására humán genetikai betegségekben
PL3586861T3 (pl) 2011-06-08 2022-05-23 Translate Bio, Inc. Kompozycje nanocząstek lipidowych i sposoby dostarczania mrna
EP3536787A1 (en) 2012-06-08 2019-09-11 Translate Bio, Inc. Nuclease resistant polynucleotides and uses thereof
PL3467108T3 (pl) * 2013-03-14 2024-09-30 Translate Bio, Inc. Sposoby oczyszczania informacyjnego rna
KR20210122917A (ko) 2013-03-14 2021-10-12 샤이어 휴먼 지네틱 테라피즈 인크. Cftr mrna 조성물 및 관련 방법 및 사용
PT2970948T (pt) 2013-03-15 2019-03-20 Glaxosmithkline Biologicals Sa Métodos de purificação de arn
CN103173438B (zh) * 2013-04-10 2015-03-04 杭州百迈生物技术有限公司 一种提取质粒的试剂和方法
ES2707966T3 (es) 2013-10-22 2019-04-08 Translate Bio Inc Terapia de ARNm para la deficiencia en síntesis de argininosuccinato
EA034103B1 (ru) 2013-10-22 2019-12-27 Транслейт Био, Инк. СПОСОБ ЛЕЧЕНИЯ ФЕНИЛКЕТОНУРИИ С ПРИМЕНЕНИЕМ мРНК
DK178664B1 (en) * 2013-10-25 2016-10-24 Amphi Consult V Martin Hesselsøe Aps A system and a method for concentrating traces of tissue from aquatic organisms in a water sample and use thereof
WO2015133972A1 (en) * 2014-03-07 2015-09-11 Agency For Science, Technology And Research Apparatus and methods for fractionation of biological products
PT3134506T (pt) 2014-04-25 2019-10-31 Translate Bio Inc Métodos de purificação de rna mensageiro
DK3294885T3 (da) 2015-05-08 2020-08-10 Curevac Real Estate Gmbh Fremgangsmåde til at fremstille rna
PL4108769T3 (pl) * 2015-05-29 2024-02-05 CureVac Manufacturing GmbH Sposób wytwarzania i oczyszczania rna obejmujący co najmniej jeden etap filtracji o przepływie stycznym
EA201991747A1 (ru) 2017-02-27 2020-06-04 Транслейт Био, Инк. НОВАЯ КОДОН-ОПТИМИЗИРОВАННАЯ мРНК CFTR
MX2019013752A (es) 2017-05-16 2020-07-20 Translate Bio Inc Tratamiento de la fibrosis quística mediante el suministro de arnm que codifica cftr optimizado en codones.
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MX2021005969A (es) 2018-11-21 2021-09-14 Translate Bio Inc Tratamiento de la fibrosis quística mediante el suministro de arnm que codifica cftr nebulizado.

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FR2705251B1 (fr) * 1993-05-13 1995-07-07 France Etat Armement Détermination de substances dans un milieu liquide par filtration tangentielle.
CA2192342A1 (en) * 1994-07-15 1996-02-01 Ann L. Lee A method for large scale plasmid purification
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Also Published As

Publication number Publication date
CN1243543A (zh) 2000-02-02
AU723817B2 (en) 2000-09-07
EP0853123A1 (de) 1998-07-15
BR9807062A (pt) 2000-05-02
AU6208498A (en) 1998-08-03
WO1998030685A2 (de) 1998-07-16
CA2277165A1 (en) 1998-07-16
EP0961826A2 (de) 1999-12-08
CA2277165C (en) 2004-03-30
KR20000069943A (ko) 2000-11-25
WO1998030685A3 (de) 1998-10-22
KR100322398B1 (ko) 2002-03-18
JP2000513580A (ja) 2000-10-17

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