TR200100899T2 - İnsan hepatit B virüsü DNA polimerazı için bir in vitro aktivite deneyi - Google Patents
İnsan hepatit B virüsü DNA polimerazı için bir in vitro aktivite deneyiInfo
- Publication number
- TR200100899T2 TR200100899T2 TR2001/00899T TR200100899T TR200100899T2 TR 200100899 T2 TR200100899 T2 TR 200100899T2 TR 2001/00899 T TR2001/00899 T TR 2001/00899T TR 200100899 T TR200100899 T TR 200100899T TR 200100899 T2 TR200100899 T2 TR 200100899T2
- Authority
- TR
- Turkey
- Prior art keywords
- dna polymerase
- hbv
- activity assay
- vitro activity
- human hepatitis
- Prior art date
Links
- 241000700721 Hepatitis B virus Species 0.000 title abstract 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 title abstract 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 title abstract 5
- 230000000694 effects Effects 0.000 title abstract 4
- 238000003556 assay Methods 0.000 title abstract 2
- 238000000338 in vitro Methods 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 229940127399 DNA Polymerase Inhibitors Drugs 0.000 abstract 1
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Mevcut bulus insan hepatit B virüsü (HBV) DNA polimerazi için bir in vitro aktivite deneyi saglar, ki bu deneyde bir örnekten elde edilmis olan HBV DNA polimerazin PCR büyütmesindeki 5' oligonükleotid'i olarak, radyoaktif olarak isaretlenmis bir ajanin varliginda PCR ürünlerini dogrudan kopyalayan ve dönüstüren ve HBV DNA polimeraz kullanima hazirlanmasini ölçen, SP6 viral (virüs kökenli) polimeraz baslaticisina birlestirilmis bir oligonükleotid'in kullanilmasini içerir. Mevcut bulus degisik serum örneklerinin aktivitesinin denenmesi, HBV DNA polimeraz önleyicilerinin elenmesi ve potansiyel anti-HBV ilaçlarinin insan HBV DNA polimeraz'in DNA hazirlama aktivitesini önleme yetenegi açisindan test edilmeleri ve/ ya da elenmeleri için bu gibi bir deneyin kullanilmasini da saglar.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/SG1998/000077 WO2000018958A2 (en) | 1998-09-29 | 1998-09-29 | An in vitro activity assay for human hepatits b virus (hbv) dna polymerase, the use of such an assay to assay activity of various serum samples and to screen for inhibitors of the hbv dna polymerase and a method of producing hbv dna polymerase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TR200100899T2 true TR200100899T2 (tr) | 2001-07-23 |
Family
ID=20429877
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TR2001/00899T TR200100899T2 (tr) | 1998-09-29 | 1999-03-13 | İnsan hepatit B virüsü DNA polimerazı için bir in vitro aktivite deneyi |
Country Status (23)
| Country | Link |
|---|---|
| US (1) | US6593082B1 (tr) |
| EP (1) | EP1117843A1 (tr) |
| JP (1) | JP2002525130A (tr) |
| KR (1) | KR100445958B1 (tr) |
| CN (1) | CN1197978C (tr) |
| AR (1) | AR030012A1 (tr) |
| AU (2) | AU9372698A (tr) |
| BR (1) | BR9914099A (tr) |
| CA (1) | CA2344282A1 (tr) |
| HK (1) | HK1039161A1 (tr) |
| HR (1) | HRP20010230B1 (tr) |
| HU (1) | HUP0103735A3 (tr) |
| IL (2) | IL142288A0 (tr) |
| IS (1) | IS5900A (tr) |
| MY (1) | MY123274A (tr) |
| NO (1) | NO20011510L (tr) |
| NZ (1) | NZ510704A (tr) |
| PL (1) | PL347026A1 (tr) |
| RU (1) | RU2202620C2 (tr) |
| TR (1) | TR200100899T2 (tr) |
| UA (1) | UA75028C2 (tr) |
| WO (2) | WO2000018958A2 (tr) |
| YU (1) | YU23601A (tr) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001278935B2 (en) * | 2000-07-14 | 2007-03-22 | Replidyne, Inc. | Novel DNA polymerase iii holoenzyme delta subunit nucleic acid molecules and proteins |
| AUPS037002A0 (en) * | 2002-02-07 | 2002-02-28 | Melbourne Health | Viral variants and uses therefor |
| KR20030075638A (ko) * | 2002-03-20 | 2003-09-26 | (주) 비엔씨바이오팜 | Ｂ형 간염바이러스의 프라이밍 저해제의 스크리닝 방법 |
| TW200643171A (en) | 2005-06-07 | 2006-12-16 | Temasek Life Sciences Lab Ltd | Porcine circovirus type 2 vaccines |
| US8859229B2 (en) | 2007-02-02 | 2014-10-14 | Yale University | Transient transfection with RNA |
| US10155038B2 (en) | 2007-02-02 | 2018-12-18 | Yale University | Cells prepared by transient transfection and methods of use thereof |
| US9249423B2 (en) * | 2007-02-02 | 2016-02-02 | Yale University | Method of de-differentiating and re-differentiating somatic cells using RNA |
| EP2512491B1 (en) | 2009-10-16 | 2017-10-11 | Glaxo Group Limited | Hbv antisense inhibitors |
| CN104316682B (zh) * | 2014-10-10 | 2015-12-30 | 三峡大学 | 一种s-腺苷甲硫氨酸脱羧酶的活性检测方法及其应用 |
| RU2763173C1 (ru) * | 2020-07-27 | 2021-12-28 | Федеральное бюджетное учреждение науки "Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. Пастера Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека" (ФБУН НИИ эпидемиологии и микробиологии имени Пастера) | Способ выявления ДНК вируса гепатита В в биологическом материале при низкой вирусной нагрузке на основе двухэтапной ПЦР с детекцией по трем мишеням в режиме реального времени |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5492817A (en) * | 1993-11-09 | 1996-02-20 | Promega Corporation | Coupled transcription and translation in eukaryotic cell-free extract |
| US5360714A (en) * | 1992-08-28 | 1994-11-01 | Fox Chase Cancer Center | Hepadnavirus polymerase gene product having RNA-dependent DNA priming and reverse transcriptase activities and methods of measuring the activities thereof |
| AU4843893A (en) * | 1992-09-02 | 1994-03-29 | Scripps Research Institute, The | Coupled isothermal polynucleotide amplification and translation system |
| US5709998A (en) * | 1993-12-15 | 1998-01-20 | The Johns Hopkins University | Molecular diagnosis of familial adenomatous polyposis |
| RU2082759C1 (ru) * | 1994-08-22 | 1997-06-27 | Центр "Биоинженерия" Ран | Штамм дрожжей saccharomyces cerevisial, содержащий рекомбинантную плазмиду yep 63/ab, - продуцент производного м-белка вируса гепатита в человека |
| WO1996010649A1 (en) * | 1994-09-30 | 1996-04-11 | Southwest Foundation For Biomedical Research | Isolated human hepatitis b virus polymerase and uses thereof |
-
1998
- 1998-09-29 WO PCT/SG1998/000077 patent/WO2000018958A2/en not_active Ceased
- 1998-09-29 AU AU93726/98A patent/AU9372698A/en not_active Abandoned
-
1999
- 1999-03-13 PL PL99347026A patent/PL347026A1/xx unknown
- 1999-03-13 TR TR2001/00899T patent/TR200100899T2/tr unknown
- 1999-03-13 CN CNB998115274A patent/CN1197978C/zh not_active Expired - Fee Related
- 1999-03-13 EP EP99910940A patent/EP1117843A1/en not_active Withdrawn
- 1999-03-13 NZ NZ510704A patent/NZ510704A/xx unknown
- 1999-03-13 UA UA2001032047A patent/UA75028C2/uk unknown
- 1999-03-13 CA CA002344282A patent/CA2344282A1/en not_active Abandoned
- 1999-03-13 HR HR20010230A patent/HRP20010230B1/xx not_active IP Right Cessation
- 1999-03-13 KR KR10-2001-7004053A patent/KR100445958B1/ko not_active Expired - Fee Related
- 1999-03-13 IL IL14228899A patent/IL142288A0/xx not_active IP Right Cessation
- 1999-03-13 YU YU23601A patent/YU23601A/sh unknown
- 1999-03-13 US US09/674,465 patent/US6593082B1/en not_active Expired - Fee Related
- 1999-03-13 WO PCT/SG1999/000017 patent/WO2000018968A1/en not_active Ceased
- 1999-03-13 HU HU0103735A patent/HUP0103735A3/hu unknown
- 1999-03-13 RU RU2001108496/14A patent/RU2202620C2/ru not_active IP Right Cessation
- 1999-03-13 JP JP2000572415A patent/JP2002525130A/ja active Pending
- 1999-03-13 HK HK02100353.9A patent/HK1039161A1/zh unknown
- 1999-03-13 AU AU29694/99A patent/AU772819B2/en not_active Ceased
- 1999-03-13 BR BR9914099-3A patent/BR9914099A/pt not_active IP Right Cessation
- 1999-09-24 MY MYPI99004150A patent/MY123274A/en unknown
- 1999-09-28 AR ARP990104895A patent/AR030012A1/es unknown
-
2001
- 2001-03-19 IS IS5900A patent/IS5900A/is unknown
- 2001-03-23 NO NO20011510A patent/NO20011510L/no unknown
- 2001-03-27 IL IL142288A patent/IL142288A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU772819B2 (en) | 2004-05-06 |
| EP1117843A1 (en) | 2001-07-25 |
| NO20011510D0 (no) | 2001-03-23 |
| CN1320167A (zh) | 2001-10-31 |
| IL142288A (en) | 2006-12-31 |
| NO20011510L (no) | 2001-05-28 |
| HRP20010230A2 (en) | 2002-06-30 |
| HUP0103735A3 (en) | 2005-12-28 |
| WO2000018968A1 (en) | 2000-04-06 |
| RU2202620C2 (ru) | 2003-04-20 |
| AR030012A1 (es) | 2003-08-13 |
| PL347026A1 (en) | 2002-03-11 |
| CN1197978C (zh) | 2005-04-20 |
| YU23601A (sh) | 2005-07-19 |
| WO2000018968A8 (en) | 2002-11-07 |
| BR9914099A (pt) | 2001-07-31 |
| CA2344282A1 (en) | 2000-04-06 |
| NZ510704A (en) | 2002-10-25 |
| HK1039161A1 (zh) | 2002-04-12 |
| US6593082B1 (en) | 2003-07-15 |
| WO2000018958A2 (en) | 2000-04-06 |
| AU9372698A (en) | 2000-04-17 |
| KR20010083879A (ko) | 2001-09-03 |
| KR100445958B1 (ko) | 2004-08-25 |
| IL142288A0 (en) | 2002-03-10 |
| UA75028C2 (en) | 2006-03-15 |
| AU2969499A (en) | 2000-04-17 |
| HRP20010230B1 (en) | 2006-05-31 |
| MY123274A (en) | 2006-05-31 |
| JP2002525130A (ja) | 2002-08-13 |
| IS5900A (is) | 2001-03-19 |
| HUP0103735A2 (hu) | 2002-01-28 |
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