TR201906978A2 - A TEXTURED REPAIR MATERIAL - Google Patents
A TEXTURED REPAIR MATERIAL Download PDFInfo
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- TR201906978A2 TR201906978A2 TR2019/06978A TR201906978A TR201906978A2 TR 201906978 A2 TR201906978 A2 TR 201906978A2 TR 2019/06978 A TR2019/06978 A TR 2019/06978A TR 201906978 A TR201906978 A TR 201906978A TR 201906978 A2 TR201906978 A2 TR 201906978A2
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- Prior art keywords
- tissue repair
- silks
- tissue
- silk
- repair material
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- 239000000463 material Substances 0.000 title claims abstract description 34
- 230000008439 repair process Effects 0.000 title description 4
- 230000017423 tissue regeneration Effects 0.000 claims abstract description 33
- 210000003491 skin Anatomy 0.000 claims abstract description 10
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 9
- 210000003205 muscle Anatomy 0.000 claims abstract description 9
- 230000006378 damage Effects 0.000 claims abstract description 8
- 241000239290 Araneae Species 0.000 claims description 16
- 210000001519 tissue Anatomy 0.000 claims description 13
- 239000011521 glass Substances 0.000 claims description 9
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 8
- 241000356536 Argiope bruennichi Species 0.000 claims description 6
- 239000002114 nanocomposite Substances 0.000 claims description 6
- 230000033001 locomotion Effects 0.000 claims description 5
- 241000254032 Acrididae Species 0.000 claims description 4
- 235000002961 Aloe barbadensis Nutrition 0.000 claims description 4
- 244000186892 Aloe vera Species 0.000 claims description 4
- 239000004471 Glycine Substances 0.000 claims description 4
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 4
- 235000004279 alanine Nutrition 0.000 claims description 4
- 235000011399 aloe vera Nutrition 0.000 claims description 4
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 3
- 235000018102 proteins Nutrition 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 239000007858 starting material Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 2
- 229920001222 biopolymer Polymers 0.000 claims description 2
- 238000009395 breeding Methods 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 claims description 2
- 230000003020 moisturizing effect Effects 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000000276 sedentary effect Effects 0.000 claims description 2
- 239000002407 tissue scaffold Substances 0.000 claims description 2
- 229920001872 Spider silk Polymers 0.000 description 11
- 230000007246 mechanism Effects 0.000 description 7
- 238000004804 winding Methods 0.000 description 7
- 208000027418 Wounds and injury Diseases 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000002086 nanomaterial Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 235000019624 protein content Nutrition 0.000 description 3
- 241000356504 Argiope Species 0.000 description 2
- 241000255789 Bombyx mori Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 238000009940 knitting Methods 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000010392 Bone Fractures Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000003869 genetically modified organism Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000002055 nanoplate Substances 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000005297 pyrex Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
- C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D7/00—Collecting the newly-spun products
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Textile Engineering (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Insects & Arthropods (AREA)
- Dermatology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mechanical Engineering (AREA)
- General Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Botany (AREA)
- Materials For Medical Uses (AREA)
Abstract
Buluş, vücut içi ve dışı yaralanmalarda, kesik, yanık ve kırık gibi hasar görmüş deri, kas ve kemik dokularda lokal tedavi ve doku onarımı amacıyla kullanılan biyolojik doku onarım malzemesi ile ilgilidir.The invention relates to biological tissue repair material used for local treatment and tissue repair in damaged skin, muscle and bone tissues such as cuts, burns and fractures in injuries inside and outside the body.
Description
TARIFNAME BIR DOKU ONARIM MALZEMESI Teknik Alan Bulus, vücut içi ve disi yaralanmalarda, kesik, yanik ve kirik gibi hasar görmüs deri, kas ve kemik dokularda lokal tedavi ve doku onarimi amaciyla kullanilan biyolojik doku onarim malzemesi ile ilgilidir. Önceki Teknik Günümüzde daha çok dogal ipek kaynakli malzeme kullanimlarinda halk arasinda ipek böcegi olarak bilinen Bombyx mori kaynakli ipekler ve bu ipeklerden elde edilen malzemeler odakli çalismalar yapilmaktadir. Bu dogal ipeklerin üstün fiziksel, biyolojik, kimyasal ve farmakolojik özellikleri dikkat çekici olmakla birlikte, örümcek ipekleri, örümceklerin agi örme amaçlarinin çok farkli olmasi nedeni ile nano yapi içerikleri ve protein içerikleri çok zengin ve farkli yapilara sahiptir. Bu nedenle yapi-özellik açisindan farklilik gösterebilen ve biyoteknolojik kazanimlara çok elverisli olan bu dogal malzemelere odaklanarak çalismalar gelistirilmelidir. Örümcek ipekleri bazi doku onarim malzemelerinin eldesinde kullanilmakla birlikte örümceklerin türü ve ipek formlari (anahat, kokon ve saklanma agi ipekleri) hakkinda bilgilere yer verilmemektedir. Oysa örümcek türü ve ipek formlari çok farkli yapisal içeriklere sahip olabilmektedir. Ayrica mevcutta kullanilan alisilagelmis ipek türleri ekonomik açidan maliyetli olmakta, örümceklerin uzun süreler yasamasi ve dogal yasamlarina geri dönebilmeleri çok mümkün görünmemektedir. Bu nedenle örümceklere ve dogal yasam ortamlarina zarar verilmeden, ekonomik ve yüksek kalitedeki örümcek ipekleri ile doku onarim malzemelerinin elde edilmesi için uygun laboratuvar kosullarinin ve donanimlarinin olusturulmasina, çiftlik yasami ortamlarina ve periyodik sagilmalara uygun, üretken örümcek türlerinin belirlenmesine ihtiyaç duyulmaktadir. dokümaninda; tip, tekstil ya da biyo-yapay hücre dokusu gibi endüstriyel uygulamalarda bir iplik ya da bir koinpozisyon olarak kullanim için örümcek ipek ipliginin kullanimindan bahsedilmektedir. Kullanilan örümcek ipegi ipligi genetigi degistirilmis organizmalardan elde edilmektedir. patent dokümaninda örümcek ipegi, özellikle de dogal örümcek ipek liflerinden olusan bir sinirsel implanttan bahsedilmektedir. Söz konusu bulus özellikle, farmasötik kompozisyonun üretimi için kullanilmak üzere örümcek ipeginin kullanilmasini mümkün kilmaktadir. Iplik benzeri örümcek ipegi, örümcek ipek ipliklerinden olusan bir kilif içinde temelde eksenel bir dogrultuda düzenlenmistir. DESCRIPTION A TISSUE REPAIR MATERIALS Technical Area The invention is damaged in internal and external injuries such as cuts, burns and fractures. used for local treatment and tissue repair in skin, muscle and bone tissues. It is related to biological tissue repair material. Prior Art Today, mostly natural silk sourced materials are used among the people. Silks originating from Bombyx mori known as silkworm and obtained from these silks Material-oriented studies are carried out. The superiority of these natural silks Although its physical, biological, chemical and pharmacological properties are remarkable, However, spider silks have very different purposes for spiders to weave webs. Due to this reason, nano structure contents and protein contents are very rich and have different structures. has. For this reason, it may differ in terms of structure-property and biotechnological Studies focusing on these natural materials, which are very suitable for gains, should be developed. Spider silks are used to obtain some tissue repair materials. together the type of spiders and their silk forms (outline, cocoon and hiding web) silks) are not included. However, spider species and silk forms can have very different structural contents. Also available The conventional silk types used are economically costly, It is very important for spiders to live for long periods and return to their natural lives. does not seem possible. Therefore, to spiders and their natural habitats. texture with economical and high quality spider silks without damage laboratory conditions suitable for obtaining repair materials and equipment, farm living environments and periodic There is a need to identify productive spider species suitable for milking. is heard. in the document; industrial type, such as textile or bioartificial cell tissue spider silk for use as a yarn or a coinposition in applications The use of yarn is mentioned. used spider silk thread derived from genetically modified organisms. in the patent document spider silk, especially from natural spider silk fibers A neural implant is mentioned. In particular, the invention of spider silk to be used for the manufacture of the pharmaceutical composition. makes its use possible. thread-like spider silk, spider silk It is basically arranged in an axial direction in a sheath consisting of threads.
Kilif örümcek ipek ipliklerinden örgü, dokuma, dantelli veya örgülü olabilmektedir. Weaving, woven, laced or braided from spider silk threads can happen.
Bulusun Kisa Açiklamasi Bu bulusun amaci, vücut içi ve disi yaralanmalarda, deri, kas, kemik gibi doku kayiplarinin oldugu vakalarda lokal tedavi ve doku onarim amaçli kullanilan biyo uyumlu bir doku onarim malzemesi gerçeklestirmektir. Brief Description of the Invention The aim of this invention is to treat tissue, such as skin, muscle, bone, in internal and external injuries. Biotechnology used for local treatment and tissue repair in cases with loss of To realize a compatible tissue repair material.
Bu bulusun bir baska amaci, Argiope brueririichi Örümceginden elde edilen üstün biyofiziksel ve biyokimyasal özelliklere sahip kokonun (koza) kullanimiyla özgün doku onarim malzemesi gerçeklestirmektir. Another object of this invention is the superior quality obtained from the Argiope brueririichi Spider. It is unique with the use of cocoon (cocoon) with biophysical and biochemical properties. tissue repair material.
Bu bulusun bir baska amaci, biyojel formunda nano kompozitlerle kaplanan kokon bölümlerinin kullanimiyla doku onarim süresi hizlandirilan bir doku onarim malzemesi gerçeklestirmektir. Another object of this invention is the coating of nanocomposites in the form of biogels. a tissue in which tissue repair time is accelerated by the use of cocoon segments repair material.
Bu bulusun bir baska amaci, örüntü düzenegi sayesinde deri, kas ve kemik gibi dokularin morfolojisine uygun doku iskelesine sahip özel tasarimli doku onarim malzemesi gerçeklestirmektir. Another aim of this invention is that, thanks to the pattern mechanism, skin, muscle and bone Specially designed tissue repair with tissue scaffold suitable for the morphology of the tissues material realization.
Bulusun Ayrintili Açiklamasi Bu bulusun amacina ulasmak için gerçeklestirilen “Bir Doku Onarim Malzemesi” için kullanilan sagim-sarim sistemi, örüntü düzenegi ve kapiler tüp ekli sekillerde gösterilmis olup; bu sekillerden: Sekil 1. Bulus konusu doku onarim malzemesi için gerekli ipek sagiminin yapildigi sagim-sarim sisteminin bir görünümüdür. Detailed Description of the Invention "A Tissue Repair Material" realized to achieve the purpose of this invention The milking-winding system, pattern assembly and capillary tube attached forms used for has been shown; of these sequences: Figure 1. Required silk milking for tissue repair material which is the subject of the invention. It is a view of the milking-winding system in which it is made.
Sekil 2. Bulus konusu doku onarim malzemesinin deri, kas ve kemik dokularinin morfolojisine uygun formda elde edilmesini saglayan örüntü düzeneginin bir görünümüdür. Figure 2. Examination of skin, muscle and bone tissues of tissue repair material which is the subject of the invention. It is an example of the pattern mechanism that enables it to be obtained in a form suitable for its morphology. is the view.
Sekil 3. Bulus konusu doku onarim malzemesinin istenilen doku morfolojisine uygun formda elde edilisini gösteren örüntü düzenegine sarim siralarina ait örneklerin bir görünümüdür. Figure 3. Desired tissue morphology of the tissue repair material of the invention. belonging to the winding rows of the pattern mechanism showing that it is obtained in the appropriate form. is a view of the samples.
Sekil 4. Bulus konusu doku onarim malzemesini kaplamada kullanilan kapiler tüp sisteminin bir görünümüdür. Figure 4. Capillary tube used in coating the tissue repair material of the invention is a view of the system.
Bulus konusu Vücut içi ve disi yaralanmalarda, kesik, yanik ve kirik gibi hasar görmüs deri, kas ve kemik dokularda lokal tedavi ve doku onarimi amaciyla kullanilan doku onarim malzemesi; agustos ayinda Türkiye°nin Dogu Karadeniz Bölgesinin sahil kesiminde yaygin olarak bulunanI popülasyonu fazla olan, üreme sikintisi bulunmayan, laboratuvar kosullarinda uzun süre yasatilabilenI farkli iklimlerde ve rakimlarda da yasayabilen, yerlesik yasama uyumlu olan ve çok üstün tiziko-biyO-kimyasal karakteristiklere sahip aglar üreten ergin Argiope bruennichi örümeeginin disi bireylerinin toplanmasi ile laboratuvar ortaminda ürettikleri kokon (koza) ve anahat formundaki ipeklerin olusturdugu en az bir baslangiç malzemesi içermektedir. Argiope bruennichi örümeeginden elde edilen bu dogal ipek malzemeler yüksek mekanik özelliklere, üstün biyolojik uyumluluga, çesitli bakteri ve mantarlara karsi güçlü biyoaktiviteye ve zararli UV isinlari sogurma özelligine sahiptir. Yerlesik yasam özelliklerinden dolayi ayni yasam kabininde optimum üç Argiope bruennichi örümcegi bulunabilmektedir. Örümceklerin sagimdan 36 saat öncesinde proteince zengin çekirge ile beslenmesi sonucu alanince ve glisince zengin olan ipek yapi elde edilebilmektedir. Çekirge ile besleme sonucu ipek yapi içerisindeki protein içerigi ve ipek saglamligina yol açan alaninee zengin kristalitlerin boyut ve sayilari artirilabilmektedir. Ayrica çekirge ile besleme sayesinde esneklik özelligi veren glisince zengin nanoplaklarin sayi ve boyutlari kontrol altina alinarak standardizasyon için uygun hale getirilmektedir. Laboratuvar ortamindaki yasain kabinlerinde uzun süre (minimum 4-5 ay) yasatilan örümcekler bu süre sonrasinda dogal yasam Ortamlarina geri salinmaktadir. Yasam ortamlarina salinan örümcekler hayatta kaldiklari süre boyunca tekrar ayni dogal yerde gözlemlenmektedir. Yani farkli sürelere göre ana yasam lokalizasyonlarinda çok az bir degisim olmaktadir. Subject of the invention Damages such as cuts, burns and fractures in internal and external injuries for local treatment and tissue repair in damaged skin, muscle and bone tissues tissue repair material used; Turkey's Eastern Black Sea Region in August It is common in the coastal part of the region, has a high population, breeding It has different that can live in climates and altitudes, compatible with settled living and very The adult Argiope produces nets with superior thiico-bio-chemical characteristics. in the laboratory environment by collecting female individuals of the bruennichi knitting Cocoon (cocoon) they produce and at least one of the silks in the outline form Contains starting material. Made from Argiope bruennichi knitting These natural silk materials have high mechanical properties, superior biological compatibility, strong bioactivity against various bacteria and fungi, and harmful UV It has the property of absorbing rays. Due to the sedentary life characteristics, the same Optimum three Argiope bruennichi spiders can be found in the living cabin. Feeding of spiders with protein-rich grasshoppers 36 hours before milking As a result, silk structure rich in alanine and glycine can be obtained. Grasshopper As a result of feeding with silk, the protein content in the silk structure and the silk firmness The size and number of alanine-rich crystallites can be increased. Moreover Rich in glycine that gives flexibility thanks to grasshopper feeding suitable for standardization by controlling the number and size of nanoplates is being made. For a long time in the law cabinets in the laboratory environment (minimum 4-5 months) They are released back into their environment. Spiders released into their habitats survive They are observed again in the same natural place during their stay. So different there is little change in the main life localizations according to the durations.
Bulus konusu doku onarim malzemesinin Argiope bruennichi Örümceginden elde edilen baslangiç malzemesi olan ipegin sagimi, bir sagim-sarim sistemi (1) kullanilarak yapilmaktadir. Sarim-sagim sistemi (1); ihtiyaç duyulan voltajin alinmasini saglayan bir regülatörü (Zi, regülatörden (2) aldigi güç sayesinde dönme hareketi elde eden elektrikli bir motoru (3), elektrikli motorun (3) ucuna yerlestirilen ve elektrikli motor (3) ile birlikte dönen içi bos cam bir tüpü (4), dönme hareketi yapan cam tüpe (4) sarilacak olan ipeklerin sagilaeagi canli ergin disi Argiope bruennichi örümceginin sabitlendigi yer olan örümcek sabitleme tablasini (5), sagilan ipeklerin sarim sayisi, boyu, hizi ve frekansi gibi parametrelerin ayarinin yapilmasini saglayan bilgisayar baglantili ve yazilim kontrollü bir kontrol birimini (6), sagimin baslatilmasini, durdurulmasini ve sonlandirilmasini saglayan tuslari (7) ve döngü hizini. döngü sonlandirma sayisini ve güncel döngü sayisini gösteren bir göstergeyi (8) içermektedir (Sekil 1). Sagim öncesinde örümceklerin ipegi kesmesini önlemek için arka ayaklari bir araya getirilip bandajlanmakta ve bandaj örümcek sabitleme tablasina (5) sabitlenmektedir. Örümcek sabitleme tablasina (5) bir veya iki örümcek sabitlenerek sagim islemi baslatilmaktadir. Regülatörden (2) alinan güç ile elektrikli motorun (3) ucuna sabitlenen cam tüp (4) döndürülerek örümcekten sagilan ipeklerin buraya sarimi saglanmaktadir. Cam tüpler (4) tercihen 15 cm boyunda ve 2-5 cm degisken çapindadir. Sarim sonucunda bobinler üzerinde belirli çap ve uzunlukta cerrahi amaçli kullanilan ipek Ilamentler (cerrahi iplikler) elde edilmektedir. Hazirlanan özgün flamentlerin mekanik özellikleri 25532±0,3 N/mm2 (stress) ve %ll,3±0,2 (strain) boyca uzama yani esneklik degerine sahiptir. The tissue repair material of the invention is obtained from Argiope bruennichi Spider. The milking of silk, which is the starting material obtained, is a milking-winding system (1) is done using Winding-milking system (1); the required voltage a regulator (Zi, thanks to the power it receives from the regulator (2)) an electric motor (3) that achieves rotational motion, to the end of the electric motor (3) a hollow glass tube (4), which is placed and rotates with the electric motor (3), The sagilaea of the silks to be wrapped in the rotating glass tube (4) are living adults. spider fixation, where the female Argiope bruennichi spider is fixed table (5), such as the number of turns, length, speed and frequency of the silk computer-connected and software that enables parameters to be set a controlled control unit (6), milking start, stop and keys (7) and the speed of the cycle. loop termination count and an indicator (8) showing the current number of cycles (Fig. 1). I'm right to prevent spiders from cutting the silk before It is brought and bandaged and the bandage spider is placed on the fixing plate (5). is fixed. One or two spiders on the spider fixing plate (5) is fixed and the milking process is started. With the power taken from the regulator (2) The glass tube (4) fixed to the end of the electric motor (3) is turned away from the spider. The silks that are supplied are provided here for winding. Glass tubes (4) preferably 15 cm tall and 2-5 cm in variable diameter. On the bobbins as a result of the winding Silk Ilaments (surgical threads) used for surgical purposes in certain diameters and lengths is obtained. Mechanical properties of prepared original filaments 25532±0.3 N/mm2 (stress) and %11,3±0,2 (strain) elongation in length, that is, flexibility value. has.
Bulusta, doku onarim malzemesini olusturan ve Ipeklerin yardimi ile elde edilen deri, kas ve kemik dokularinin morfolojisine uygun doku iskelelerinin hazirlamasini saglayan bir örüntü düzenegi kullanilmaktadir. Cam tüplere (4) sarili ipeklerden örüntü düzenegi sayesinde cerrahi amaçli kullanilan yara greftleri elde Edilmektedir. Örüntü düzenegi; dairesel olan ve dönme hareketi yapabilen üst kisim ve farkli sekil ve boyutlarda cisimlerden dengeyi koruyacak sekilde olusturulan ve üst bölümü tasiyan bir platform kismi olmak üzere iki kisimdan Olusmaktadir. Örüntü düzeneginin üSt kisminda dairesel yapisinin kenarlarinda esit araliklarla 0, dan 1 l , e kadar numaralandirilmis 12 adet çikinti bulunmaktadir (Sekil 2). Ipekler örüntü düzenegi çikintilarinin etrafindan doku morfolojisini elde etmeye uygun siralar ile geçirilmekte ve onarilmak istenen dokunun morfolojisine uygun bir greft elde edilmektedir. Sarim sirasina örnek olarak 0-6-5-l0-11-4-3-0- (Sekil 3). Söz konusu örüntü düzenegi; borosilikatli (B203 içeren) korozyona dayanikli, düsük genlesme kat sayisina sahip, yüksek sicakliklara ( dayanikli camlardan pyrex malzeme kullanilarak, isil islem ile sekillendirme, kesme ve yapistirma islemleri ile olusturulmaktadir. 20x20 mm boyutlu hazirlanan greft malzemenin UV geçirgenligi ve reflenktansi ölçüldügünde UV sogurma miktarinin 355-400 nm dalga boyunda %70 civarinda oldugu belirlenmektedir. In the invention, the tissue repair material and obtained with the help of Silks. scaffolds suitable for the morphology of skin, muscle and bone tissues A pattern mechanism is used that enables the preparation of into glass tubes (4) Wound grafts used for surgical purposes thanks to the pattern mechanism made of wrapped silk is obtained. Pattern arrangement; circular top, capable of rotational movement in a way to maintain balance from parts and objects of different shapes and sizes. It consists of two parts, a platform part formed and carrying the upper part. is occurring. At the edges of the circular structure at the top of the pattern assembly There are 12 protrusions numbered from 0 to 1 l with equal intervals. (Fig. 2). Obtain tissue morphology around the silks pattern assembly protrusions. It is passed with the appropriate rows and the morphology of the tissue to be repaired. a suitable graft is obtained. As an example of the winding order 0-6-5-l0-11-4-3-0- (Figure 3). The pattern mechanism in question; borosilicate (containing B203) corrosion durable, low expansion coefficient, high temperatures ( Forming with heat treatment by using pyrex material from durable glasses, It is formed by cutting and pasting processes. 20x20 mm size When the UV transmittance and reflectance of the prepared graft material is measured, the UV Absorption amount is around 70% at 355-400 nm wavelength. is determined.
Bulus konusu cam tüplere (4) sarilarak ve örüntü düzenegi kullanilarak cerrahi iplik (flament) ve greft gibi doku malzemeleri elde edilmektedir. Doku malzemelerinin üzeri biyoaktivitelerini güçlendirmek için tamami ile dogal ipek mikro tozlari ile hazirlanan nemlendirici ve doku onarici özellikteki aloe vera ve bitkilerden elde edilen biyo polimer olan levan nano kompozitleri ile kaplanmaktadir. Aloe vera ve levan biyojelleri kokonun temizlenip ögütülmesi ile elde edilen Ipek mikro tOZlari ile belirli oranlarda birlestirilip santrifüj ile karistirilarak homojen nano kompozit jeller elde edilmektedir. Anahat ve kokon ipekleri ile hazirlanan greft ve cerrahi iplikler aloe vera ve levan biyojelleri ile dogrudan kaplanmayip daha özgün ve etkin doku onarimlari için jel formundaki nanokompozitler ile kaplama islemine tabi tutulmaktadirlar. Böylece sadece ipek hatlarin oldugu örüntü bölgeleri ile sinirli kalinmayip nemli jel yapi içinde uniform dagilmis nano yapilarin etkisi ile iyilesme süreci daha da hizlandirilmaktadir. Greftler, cam tüplere (4) sarilan ipeklerin örüntü düzeneginde uygun morfolojik yapilara getirilmesinin ardindan bu düzenek üzerine nano kompozit jellerin damlatilarak santrifüj islemi ile emdirilmesi sayesinde kaplanmaktadir. Cerrahi iplikler ise Çelik igneye geçirildikten sonra, kapiler tüp içine hiçbir hava boslugu kalmayacak sekilde doldurulan nano kompozit içinden geçirilerek kaplanmaktadir (Sekil 4). Kaplamada kullanilan Örnekler, doku onarimlarini hizlandirinakta ve daha etkin iyilesme saglayarak ipek doku arayüzünde antimikrobiyal, antifungal ve yara iyilestirici biyoaktivitelerden faydalanarak dokunun dogal nem ortaminin korunmasini saglamaktadir. Kaplama sonrasinda doku onarim malzemesi, sahip oldugu mekanik özellik dolayisiyla yara ile bütünlesmeyi saglayacak yeterlilige gelmekte, elektriksel iletkenligi sebebiyle yara iyilesme sürecinde faydali olmakta, UV isini sogurma özelliginden dolayi günesin zararli isinlarina karsi bir engel olusturarak yanik tedavisinde ve deri doku onariminda etkin olan antibakteriyel özellikli dogal protein kaynagi haline gelmekte, kemik kiriginda veya hasarinda hidroksi apatit gibi doku onariminda kullanilabilecek dogal destek malzemeleri ile `biyo uyumluluk göstermekte ve kristalin yapi gelisimine alt köprü ve zemin olusturmaktadir. Surgery is performed by wrapping the inventive glass tubes (4) and using the pattern assembly. Tissue materials such as filaments and grafts are obtained. Tissue completely natural silk to strengthen the bioactivity of the materials Aloe vera with moisturizing and tissue repairing properties prepared with micro powders and with levan nanocomposites, which are biopolymers obtained from plants. is covered. Aloe vera and levan biogels are produced by cleaning and grinding the coco. It is combined with the obtained Silk micro powders in certain proportions and centrifuged. homogeneous nanocomposite gels are obtained by mixing. outline and cocoon grafts and surgical threads prepared with silks are combined with aloe vera and levan biogels. It is not directly coated, but in gel form for more specific and effective tissue repairs. They are coated with nanocomposites. So just silk It is not limited to the pattern areas where the lines are, but in a moist gel structure. The healing process is further enhanced by the effect of uniformly dispersed nanostructures. is accelerated. The grafts are in the pattern arrangement of the silks wrapped in the glass tubes (4). After it was brought to the appropriate morphological structures, this mechanism was applied to the nano Thanks to the absorption of the composite gels by centrifugation by dripping, is covered. Surgical threads, on the other hand, are inserted into the steel needle after the capillary tube. It is made of nano composite, which is filled in such a way that there is no air gap. and covered (Figure 4). Samples used in coating, texture Silk texture accelerates the repair and provides more effective healing. of antimicrobial, antifungal and wound-healing bioactivities at the interface It ensures the preservation of the natural moisture environment of the tissue by taking advantage of it. Covering tissue repair material, due to its mechanical properties, due to its electrical conductivity, It is beneficial in the wound healing process, due to its UV light absorption feature. by creating a barrier against the harmful rays of the sun in the treatment of burns and skin It becomes a natural protein source with antibacterial properties, which is effective in tissue repair. in tissue repair, such as hydroxy apatite in bone fracture or damage. It shows biocompatibility with the natural support materials that can be used and It forms a lower bridge and ground for the crystalline structure development.
Bulus konusu hazirlanan greft ve ilament gibi doku onarim malzemeleri cerrahi uygulamalar için morfolojilerine, örüntülerine, anahat sayilarina, anahat Çaplarina, Ilament boylarina, protein içeriklerine, alanin ve glisince zengin nano yapi dagilimlarina göre siniflandirilarakj paketlenmekte ve kullanim öncesi 10 kGy” lik gamma radyasyonu ile steril edilmektedir. Bu düsük doz ile yapilan strelizasyon sonucunda yapiya moleküler ve nano ölçekte zarar verilmemektedir. Tissue repair materials such as grafts and ilament prepared as the subject of the invention their morphologies, patterns, outline numbers, outline Diameters, Nanostructure rich in filament lengths, protein contents, alanine and glycine Classified according to their distribution, they are packaged and 10 kGy” sterilized by gamma radiation. Sterilization with this low dose As a result, the structure is not damaged at molecular and nano scale.
Bu temel kavramlar etrafinda, bulus konusu doku onarim malzemesi için çok çesitli uygulamalarin gelistirilmesi mümkün olup, bulus burada açiklanan örneklerle sinirlandirilamaz, esas olarak istemlerde belirtildigi gibidir.Around these basic concepts, the subject matter of the invention is very useful for tissue repair material. It is possible to develop various applications, the invention being described herein. not limited to examples, essentially as specified in the claims.
Claims (1)
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| PCT/TR2020/050388 WO2020226595A1 (en) | 2019-05-09 | 2020-05-06 | A tissue repair material |
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