TR201910569A2 - DESIGN AND PRODUCTION OF PARTIAL GRAVITY PLATFORM USING MAGNETIC FORCES FOR CELLULAR APPLICATIONS - Google Patents

Abstract

The invention relates to a cell culture platform that can test the morphological and functional adaptations of cells subjected to gravity in different planets and satellites by reducing the weight forces on living cells designed using magnetic principles to generate information in the field of space biology.

Description

DESCRIPTION THE PART USING MAGNETIC FORCES FOR CELL APPLICATIONS GRAVITY PLATFORM DESIGN AND MANUFACTURING Technical Field of the Invention The invention is carried out using magnetic principles to generate knowledge for the field of space biology. It is about a biotechnological platform that enables engineered cell culture.

State of the Art of the Invention (Prior Art) Mechanical forces play a role in the formation of living tissue and saglllZllZl form and function. It is located between the basic parameters that play and these mechanical forces are eliminated. Kaltlgllîl conditions can create a yearly Handle Effect for living Zlldtissues. This year ZIEE effect, people. as the biggest obstacle to long-term stays in spaceII. is seen. Understanding the adaptation of human cells, tissues and organsII aglEIIEIleE to the environment For this purpose, many cell- or organism-based biotechnological methods are widely used. However, this is one of the most important long-term goals of space travelII. colonization of a satellite or planet at the end of a voyage. For example Moon (g=1.6 m/sz) or on astronomical objects such as Mars (g=3.7 m/sz) sulîljan has different gravitational attraction. In the state of the art, these objects colonization began on their surface [the glilia are people who will adapt or be born there. Technologies that will help to make predictions about the biological adaptations it will undergo are quite is limited.

Although the subject of space biotechnology is widely discussed, it is of strategic importance. tasliaktadlE Humanitarian activities such as space-related activities, communication, observation, exploration and defense As it can be done, the human existence in space at an increasing rate[g]Il10 It is expected to live in Elî.

Scientists working on space biology. common motivation of gravity whether it is possible to develop the space differentiilioktalara canll]]1g]I change The current scientific studies carried out in this context are scientific studies with a very high impact value. çllîtlllar can present. Despite this, the work done on space biology or completely on the aglElllllelîl environment or completely ignoring the gravitational effects carried out over chemical and thermodynamic compositions.

In principle, the gravitational forces acting on the object are applied in the opposite direction. With the magnetic force toolUJgEIII, the object's weight can be significantly reduced (magnetic lightening) so that different gravitational pulls on moons and planets can be modeled. known. In the state of the art, magnetic forces are the Ievitation of bodies. It is used to provide the relevant gravitational pull, which is used and reduced A magnetic attenuation technique that provides graduated control of forces has previously been In the aforementioned prior art, Ebatent document number CN106471370 a heterogeneous population of cells in a magnetically sensitive environment and magnetic field positions in the gradient where the magnetic force and gravitational forces are balanced The invention in question describes a system that can know the moon by making use of different hands. In this system, the cells are separated and fully suspended, bent and folded. Therefore, gravity decreases clinically, which is effective in different Epstronomic environments. It does not allow the interrelationship of the biological effects of variable gravity.

Purposes of the Invention The primary purpose of the invention is to detect the gravity of the satellite or planet whose colonization is planned. to model in the world.

With the invention, by reducing the aglEllllZ] forces on canllZ cells, different planets and planets can be formed. Morphological and functional adaptations of gravitational cells in satellitesll] A cell culture platform has been created to test it.

The cell culture platform, which is the subject of the invention, differs under changing gravitational conditions. textures. Behave to the testing of hypotheses about Elarliness with the possibility of analysis at the cellular level. enablingdlB With the invention, the pain formed on all the masses exposed to gravity on Earth in the opposite direction of the force and at the desired rate by means of magnetic forces whose intensity can be adjusted. i am underestimating Invention platform is the planet and planet with the possibility of colonization in the solar system. Mechanical adaptations of living cells on satellitesHand modeling on-an It has the feature of being the first product designed.

Invention is a much easier and cheaper platform for experiments carried out in space. presents, facilitates the production of knowledge on the subject of space biology, and facilitates the production of information on the subject of space biology aims.

The invention also carries the potential to be used in different applications at the same time. This set of systems and methods that can manipulate forces on a diamagnetic body biology studiesStudies examining the biological effects of hand/e mechanical forces. sideElslß Potential to influence other research using micro-manipulation as a tool For example, it should come into contact with biological structures and provide integrity with tissue. biomaterial-cell, which is extremely critical in the development of expected biomaterials It is important for many health applications such as interaction strength, biological test systems. molecular affinity can be studied by the principle to be defined in this invention.

The Gods of Figures Opening the Invention Prepared for better understanding of the cell culture platform developed by this invention. Figures are opened below Figure 1: Before and after equilibration of a spherical particle in a paramagnetic environment forces acting on it (Prior Art).

Figure 2: With the effect of magnetic force vector (FM) and gravity vector (F9); magnetic Figure 3: Cylindrical and perforated type neodymium wirelines positioned between the ElBwEl cell Invention consisting of a culture platform providing partial loading of aglillilyl on cells Figure 4: The cylindrical and perforated type neodymium m[lZline|5 cells positioned between EllBilSl (A) 30, (B) 45 and (C) 60 mM Gd3+ cells with a density of 1.05 g/mL suspended in medium containing On the mlLZhatE surface and at certain distances from the lower mlLZhatEtan (a: mlEhatE surface, b: 0.5 mm Distance c: Graphics showing the total acceleration acting at a distance of 1 mm [lZl d: at a distance of 1.5 mm [lZl].

Change in acceleration on cells with COMSOL Multiphysics modeling yaz[l]]iiil:l has not been resolved.

Figure 5: Cylindrical and perforated type neomydium mllZhatlglar positioned EIIBiE cell (A) 30, (B) 45 and (C) 60 mM Gd3+ cells with a density of 1.1 g/mL suspended in medium containing mllZhatlîl surface and lower milZhatEtan at certain distances (a: m[lZhatlg surface, b: 0.5 mm Graphs showing the total acceleration acting at a distance of c: 1 mm at a distance of d: 1.5 mm at a distance of KD.

Acceleration change on cells with COMSOL Multiphysics modeling software Elilîl has not been resolved.

Invention ElementsIII/KIEIIE[Parts GodsHand By reducing the network forces on living cells developed with this invention, different morphological and functional cells of gravitational cells on planets and moons In order to better explain the cell culture platform that can test the adaptations The parts/things/elements in the hazliibnan figures are AyrEayrEhumaralandIElBiEI and each A number is given to the Elamasßsag-. 1: Perforated Neodymium (N52) M[Ehatlîl 2: Cell Culture Platform 3: Cell Culture LamEi 4: YapEkan Band : Drilled Polymer Well D: Balance Position Z: Cell Culture Chamber Base Detailing the Invention Magnetic principles: On the floor (Z) of the cell culture chamber inside the silica Equilibrium in the system on a particle (or cell) located under the influence of magnetic force net force (FA/et) until position (D): magnetic force (FM), drift force (FD) and gravitational force (FG) (set of equation 1) (Figure 1). In this system, inertial force (F,) low Reynolds sayEEl in microfluidic magnetophoresis Because of this, the Brownian force (F5) only contains sufficiently small particles (approximately) 510 nm) can be ignored because it significantly affects the motion.

If FNet=îd+F7+F7 then ma=îj+îJ+Fi FM acting on a particle; B: magnetic intelligence density (Tesla, T), V: del operator, ü : depending on the magnetic dipole valuesIIE (equation 2). B milZhat- from the surface It decreases with distance. From here in paramagnetic salt solution or in ferrous The magnetic dipole produced in the low magnetic field can be reached (equation 3).

H,“:mvß (2) and magi (3) In this equation; i/E particle[g]I volume, po: permeability of space (1.2566 x10'6 kg'm'A'Z's'z), Ax: the magnetic susceptibility (magnetic susceptibility) of the particlecllZl and the surrounding environment FM value can be expressed by arranging susceptibility as different (equation 4).

(Expanding E. @E in the Cartesian coordinate system: (B. V)B= kßxaîwyîyw'î_y) (5) magnetic intelligence values can be reached in three dimensions. under specified conditions, a global Drag force FD, which is another force acting on particles; R (particle diameter n (dynamic viscosity), @(5 drag coefficient and vpj/e (particiglliîl hlîm) will vary depending on (equation 6). = 6nRnfd(vp) (6) If the other force acting on the particle is FG; I/: piececlgll volume, Ap : piececlGll with it volumetric density difference in the surrounding environment, g. gravitational acceleration (9.8 m'z) relative can be calculated as (equation 7).

F7 = Vûpg (7) In this case, the force acting on it, until the particle equilibrium point (D) is reached. (Equation 8) to reach the equilibrium point (D) (FD: 0) FNet can be arranged according to the global particle equilibrium state (equation 9).

FNet - +m)(B V)B + 6uRnfd(vp) + V(pp- :w )9 (8) and Particles that are diamagnetic like cells are magnetic when a magnetic field is applied. It creates a magnetic dipole aligned antiparallel to the field, and therefore the magnetic dipole A magnetic force is formed towards the area where the area is minimum. The dipole, on the other hand, is based on the magnetic susceptibility value of the particles and the surrounding environment. is changing. In the invention, by making use of the physical events explained in detail above, the magnetic force acting on the cells in the platform and therefore the net magnetic susceptibility value (xm) and magnetic flux It is provided by changing the density (B) of the magnetic medium surrounding the cells. emotional value, nutrient medium. By adding paramagnetic ions at different concentrations, the environment & magnetic flux density and distribution in which cells are found mllZhatElarI is obtained by placing them in different shapes. As a result, cells By reducing the gravitational force acting on it to the desired extent, this change is biologically A platform that can be used for researching its effects is presented (Figure 2). It is a gravity platform that uses magnetic forces for cellular applications. perforated type neodymium located on mllZhatlîl (1) acting on the cells Cell culture, which reduces the net force by creating a magnetic force in the opposite direction to gravity A cell culture platform (2) with a punctured polymer well (5) located on lamII is characterized as.

The aforementioned cylindrical and perforated type neodymium mllZhat- (1) both formed The magnetic force vector is regular and high, and the hole in the middle is in the microscope. Allows direct cell viewing. Cell placed on MilZhat- (1) adhesive tape on culture platform (2), cell culture larnII (3) (cell culture surface) (4) from a precision-drilled polymer well (cell culture chamber) converging with (5) mllZhatlgan (1) distance[g] of the cell culture surface It is a factor that determines the severity of the reverse acceleration on the cells. This is reverse acceleration mEIZhatlEtan (1) decreases with distance This system, which can create directional acceleration and provides partial loading; cell culture (2) bottom of the platform. and on top of it, with the opposite poleshand facing each other. positionedEIJ-:hlg perforated N52 neodymium rods (1) consisting of 0(Fig. 3). This on the platform, on the lower mlZhatE surface and on the lower miIZhatEtan, which acts on cells at certain distances. The acceleration is analyzed with the COMSOL Multiphysics modeling software]]]ilil:ll. having different/health In order to show that aglEllllZl forces on cells can be reduced by this invention, this effect on cells with a density of 1.05 g/mL (Figure 4) and 1.1 g/mL (Figure 5) with the system. acceleration is modeled. Do this modeling[[[lîlken cells three differentIEGadoIinium (III) (Gd3+) in the sub-mlEhatlgl surface when suspended in concentration and The acceleration acting on the cell is modeled at 0.5, 1 and 1.5 mm distances from the surface.

The positive acceleration values in the graphs (Figure 4 and Figure 5) are the same/leading with the gravitational force and The results will reduce the forces of AgEI on cells at selected surfaces and distances. The figure shows that the required acceleration can be provided by the system.

As explained above, which can create acceleration in the opposite direction to gravity on the cells. system in a similar way; (I) the bottom of the cell culture platform only and (ii) separation poles into the bottom, hole of the cell culture platform. mEhatEI with holes in another cylindrical position, facing each other It can also be created by placing

Claims (1)

REQUESTS . It is a gravity platform that uses magnetic forces for cellular applications. a cell culture platform (2) with a punctured polymer well (5) located on the cell culture glass (3), which reduces the net force acting on the cells with a magnetic force in the opposite direction to gravity, positioned on at least one miighatlil (1). ) is characterized as . It is a cell culture platform according to claim 1 and its feature is; the bottom of the cell culture platform (2). and on top of it, cylindrical and/or perforated type neodymium milia- It is a cell culture platform according to claim 1 and its feature is; is to connect the cell culture platform with the cell culture IamEi to the adhesive tape (4) on the said IamI. . The cell culture platform is the method of preparation and its feature is; The magnetic force acting on the cells in the platform is increased and therefore the net force is decreased. The nutrient medium should be provided by changing the magnetic saturation value (xm) and magnetic flux density (B) . It is the method of preparation of the cell culture platform in accordance with claim 4 and its feature is; The nutrient medium surrounding the cells II is the difference of the magnetic saturation values (xm), nutrient medium. It is provided by adding paramagnetic ions at different ZI concentrations. It is a cell culture platform preparation method in accordance with claim 4 and its feature is; The environment in which the cells are located is obtained by placing them in at least one miEhat- different effects.