TW200512295A - Method for the gene transfer mediated by implused magnetic fields - Google Patents

Method for the gene transfer mediated by implused magnetic fields

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Publication number
TW200512295A
TW200512295A TW092126270A TW92126270A TW200512295A TW 200512295 A TW200512295 A TW 200512295A TW 092126270 A TW092126270 A TW 092126270A TW 92126270 A TW92126270 A TW 92126270A TW 200512295 A TW200512295 A TW 200512295A
Authority
TW
Taiwan
Prior art keywords
gene transfer
cells
gene
dna
nucleic acid
Prior art date
Application number
TW092126270A
Other languages
Chinese (zh)
Inventor
Wen-Chien Lee
Ming-Yau Chen
Tzu-Hsien Wang
Original Assignee
Wen-Chien Lee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wen-Chien Lee filed Critical Wen-Chien Lee
Priority to TW092126270A priority Critical patent/TW200512295A/en
Publication of TW200512295A publication Critical patent/TW200512295A/en

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  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A method is disclosed for transferring genetic materials like DNA into cells by applying a strong magnetic field in pulses. To mediate the gene transfer, cells and nucleic acid construct are mixed and exposed in pulsed magnetic field once or several times. Cells receiving the DNA in the cytoplasm and then nuclei, where the gene products encoded in DNA are expressed transiently or continuously. The nucleic acid construct can be either magnetic or non-magnetic. However, the use of superparamagnetic nanoparticles for the formation of nucleic acid construct, the efficiency of gene transfer can be promoted. The present invention discloses a simple and fast method for gene transfer to cells for the application in gene therapy, gene transfcetion, and drug delivery.
TW092126270A 2003-09-23 2003-09-23 Method for the gene transfer mediated by implused magnetic fields TW200512295A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW092126270A TW200512295A (en) 2003-09-23 2003-09-23 Method for the gene transfer mediated by implused magnetic fields

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW092126270A TW200512295A (en) 2003-09-23 2003-09-23 Method for the gene transfer mediated by implused magnetic fields

Publications (1)

Publication Number Publication Date
TW200512295A true TW200512295A (en) 2005-04-01

Family

ID=57798605

Family Applications (1)

Application Number Title Priority Date Filing Date
TW092126270A TW200512295A (en) 2003-09-23 2003-09-23 Method for the gene transfer mediated by implused magnetic fields

Country Status (1)

Country Link
TW (1) TW200512295A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11442117B2 (en) 2016-11-09 2022-09-13 Sigma Genetics, Inc. Systems, devices, and methods for electroporation induced by magnetic fields

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11442117B2 (en) 2016-11-09 2022-09-13 Sigma Genetics, Inc. Systems, devices, and methods for electroporation induced by magnetic fields

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