TWI751154B - 一種重組人粒細胞集落刺激因子的製備方法 - Google Patents

一種重組人粒細胞集落刺激因子的製備方法 Download PDF

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TWI751154B
TWI751154B TW106112759A TW106112759A TWI751154B TW I751154 B TWI751154 B TW I751154B TW 106112759 A TW106112759 A TW 106112759A TW 106112759 A TW106112759 A TW 106112759A TW I751154 B TWI751154 B TW I751154B
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expression vector
stimulating factor
granulocyte colony
human granulocyte
recombinant human
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TW106112759A
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Chinese (zh)
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TW201738263A (zh
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喻馗
王宏偉
田靜
曾翔
孔祥林
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大陸商江蘇恆瑞醫藥股份有限公司
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
TW106112759A 2016-04-18 2017-04-17 一種重組人粒細胞集落刺激因子的製備方法 TWI751154B (zh)

Applications Claiming Priority (2)

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CN201610241276 2016-04-18
CN201610241276.8 2016-04-18

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TW201738263A TW201738263A (zh) 2017-11-01
TWI751154B true TWI751154B (zh) 2022-01-01

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CN (1) CN108368512B (fr)
TW (1) TWI751154B (fr)
WO (1) WO2017181920A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110066331B (zh) * 2018-01-23 2022-05-24 江苏恒瑞医药股份有限公司 一种重组人粒细胞集落刺激因子的制备方法
CN113862293B (zh) * 2020-06-30 2023-06-09 中国科学院广州生物医药与健康研究院 α-硫辛酸的生物合成方法、工程菌株及其制备方法
CN112779264A (zh) * 2021-01-04 2021-05-11 集美大学 一种重组表达粒细胞集落刺激因子的工程菌株及其应用
CN119842769A (zh) * 2025-01-21 2025-04-18 中国农业科学院上海兽医研究所(中国动物卫生与流行病学中心上海分中心) 重组猫巨噬细胞集落刺激因子的原核表达质粒及其构建方法和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104689333A (zh) * 2007-08-27 2015-06-10 拉蒂奥法姆有限责任公司 G-csf缀合物的液体制剂
CN105273076A (zh) * 2014-06-18 2016-01-27 江苏奥赛康药业股份有限公司 一种聚乙二醇化重组人粒细胞刺激因子的制备方法及其药物组合物

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100694994B1 (ko) * 2005-06-13 2007-03-14 씨제이 주식회사 사람 과립구 콜로니 형성인자 동종체
CN101766810A (zh) * 2008-12-29 2010-07-07 北京双鹭药业股份有限公司 一种含有重组人粒细胞集落刺激因子的药物制剂
KR101831300B1 (ko) * 2010-10-29 2018-02-23 한미사이언스 주식회사 재조합 대장균으로부터 인간 과립구 콜로니 자극인자를 정제하는 방법
HUP1200172A2 (en) * 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
CN105349578A (zh) * 2015-11-30 2016-02-24 肇庆大华农生物药品有限公司 一种鸡gm-csf蛋白及其制备方法和应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104689333A (zh) * 2007-08-27 2015-06-10 拉蒂奥法姆有限责任公司 G-csf缀合物的液体制剂
CN105273076A (zh) * 2014-06-18 2016-01-27 江苏奥赛康药业股份有限公司 一种聚乙二醇化重组人粒细胞刺激因子的制备方法及其药物组合物

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
付瑤，"人粒細胞集落刺激因子的基因合成、原核表達與活性研究"，吉林大學碩士學位論文，2011年6月 *

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CN108368512A (zh) 2018-08-03
WO2017181920A1 (fr) 2017-10-26
TW201738263A (zh) 2017-11-01
CN108368512B (zh) 2020-03-27

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