TWI907038B - Microfluidic detection system with self-standard benchmark and rapid deployment of artificial intelligence identification and method thereof - Google Patents

Microfluidic detection system with self-standard benchmark and rapid deployment of artificial intelligence identification and method thereof

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TWI907038B
TWI907038B TW113132336A TW113132336A TWI907038B TW I907038 B TWI907038 B TW I907038B TW 113132336 A TW113132336 A TW 113132336A TW 113132336 A TW113132336 A TW 113132336A TW I907038 B TWI907038 B TW I907038B
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artificial intelligence
identification
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TW202609299A (en
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簡銘伸
葉怡玲
張育榕
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國立虎尾科技大學
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Abstract

A microfluidic detection system with self-standard benchmark and rapid deployment of artificial intelligence identification includes a sensing device, a microfluidic chip and a processor. The microfluidic chip corresponds to the sensing device and includes a plurality of injection holes, a standard part, an observation part, a plurality of microfluidic channels and an identification part. The standard part includes a standard product and has a standard space, and the standard product is disposed in the standard space. The observation part has an observation space. Two of the injection holes, the standard part and the observation part are connected to each other by one of the microfluidic channels. The identification part has an identification mark, and the identification mark is configured to be sensed by the sensing device to identify mark information. The processor is signally connected to the sensing device and the microfluidic chip, and includes an artificial intelligence identification module. The artificial intelligence identification module receives the mark information from the sensing device, and automatically imports a corresponding artificial intelligence identification process according to the mark information, and converts an observation result of the standard space into a calculation parameter according to the artificial intelligence identification process, and automatically identifies the observation space according to the calculation parameter to generate an experimental result. Therefore, the present disclosure utilizes artificial intelligence to shorten detection time and reduce manual detection errors.

Description

具有自標準基準及快速部署人工智慧辨識之微流道檢測系統及其方法A microfluidic channel detection system and method with self-standard benchmark and rapid deployment of artificial intelligence recognition.

本發明是關於一種微流道檢測系統及其方法,特別是關於一種具有自標準基準及快速部署人工智慧辨識之微流道檢測系統及其方法。This invention relates to a microchannel detection system and method, and more particularly to a microchannel detection system and method with self-standard criterion and rapid deployment of artificial intelligence identification.

隨著檢測科學之進步,目前除了透過實驗室的專業實驗流程進行檢測外,亦發展出許多方便使用的檢測試片。習知的檢測試片通常包含檢測槽,檢測槽中容置有特定試劑,試劑的種類取決於檢測的目標物質。使用習知的檢測試片時,僅需將檢體加入檢測槽中,待檢體與試劑反應完畢後觀察或測量試劑的性質變化,即可得知檢測結果。With advancements in testing science, in addition to professional laboratory procedures, many user-friendly test strips have been developed. Conventional test strips typically include a test chamber containing a specific reagent, the type of which depends on the target substance being tested. When using conventional test strips, simply add the sample to the test chamber, and after the reaction between the sample and reagent is complete, observe or measure the changes in the reagent's properties to obtain the test result.

然而,習知的檢測需透過人工檢測,其所需之檢測時間冗長,且檢測流程較為繁瑣。此外,人工檢測容易導致人工判讀的誤差及標準不一致。由此可知,目前市場上缺乏一種可有效縮短檢測時間並減少人工判讀誤差的具有自標準基準及快速部署人工智慧辨識之微流道檢測系統及其方法,故相關業者均在尋求其解決之道。However, conventional inspection requires manual inspection, which takes a long time and the inspection process is cumbersome. In addition, manual inspection can easily lead to errors in manual interpretation and inconsistent standards. It can be seen from this that there is currently a lack of a microfluidic detection system and method on the market that can effectively shorten the detection time and reduce manual interpretation errors, with self-standard benchmarks and rapid deployment of artificial intelligence identification. Therefore, relevant industries are looking for solutions.

本發明之目的在於提供一種具有自標準基準及快速部署人工智慧辨識之微流道檢測系統及其方法,可用於具有物理與化學的生物細胞、生物化學等實驗,藉由標誌資訊之辨識可自動導入對應之人工智慧辨識程序,並透過人工智慧辨識程序在觀察區域進行自動化辨識而快速取得檢測結果,可有效縮短檢測時間並減少人工判讀的誤差,以解決習知檢測時間冗長及人工判讀誤差的問題。The purpose of this invention is to provide a microfluidic channel detection system and method with self-standard benchmarks and rapid deployment of artificial intelligence recognition. It can be used in experiments involving physical and chemical biological cells, biochemistry, etc. By identifying the label information, the corresponding artificial intelligence recognition program can be automatically imported, and the detection results can be quickly obtained through automated identification in the observation area by the artificial intelligence recognition program. This can effectively shorten the detection time and reduce the error of human interpretation, so as to solve the problems of long learning detection time and human interpretation error.

依據本發明的結構態樣之一實施方式提供一種具有自標準基準及快速部署人工智慧辨識之微流道檢測系統,其包含一感測裝置、一微流道晶片以及一處理器。微流道晶片對應感測裝置且包含複數注入孔、一標準部、一觀察部、複數微流道及一識別部。標準部包含一標準品且具有一標準品空間,標準品設置於標準品空間。觀察部具有一觀察空間。此些注入孔、標準部及觀察部之二者透過此些微流道之一者連通。識別部具有一識別記號,識別記號用以供感測裝置感測而識別出一標誌資訊。處理器訊號連接感測裝置與微流道晶片,且包含一人工智慧辨識模組。人工智慧辨識模組接收來自感測裝置之標誌資訊,並依據標誌資訊自動導入對應之一人工智慧辨識程序,並依據人工智慧辨識程序將標準品空間的一觀測結果轉換成一計算參數,並依據計算參數自動辨識觀察空間而產生一實驗結果。According to one embodiment of the present invention, a microchannel detection system with self-standard datum and rapid deployment of artificial intelligence recognition is provided, comprising a sensing device, a microchannel chip, and a processor. The microchannel chip corresponds to the sensing device and includes a plurality of injection ports, a standard section, an observation section, a plurality of microchannels, and an identification section. The standard section includes a standard sample and has a standard sample space, in which the standard sample is disposed. The observation section has an observation space. The injection ports, the standard section, and the observation section are connected through one of the microchannels. The identification section has an identification mark for the sensing device to detect and identify a mark information. The processor is signal-connected to the sensing device and the microchannel chip and includes an artificial intelligence recognition module. The AI recognition module receives the tag information from the sensing device and automatically imports the corresponding AI recognition program based on the tag information. Based on the AI recognition program, it converts the observation result of the standard space into a calculation parameter and automatically identifies the observation space based on the calculation parameter to generate an experimental result.

前述實施方式的其他實施例如下:前述標準品包含一物理性物件或一化學性物件,標準品空間與觀察空間之任一者為一三維空間,三維空間為球體或方體。標準部與觀察部之一者具有透光性及低光線散射性。此些注入孔之一者與標準部透過此些微流道之此者連通。識別記號包含一射頻標籤、一二維條碼、一維條碼或一文字。Other embodiments of the aforementioned implementation method are as follows: The aforementioned standard includes a physical object or a chemical object, and either the standard space or the observation space is a three-dimensional space, which is a sphere or a cube. One of the standard part and the observation part is transparent and has low light scattering. One of these injection holes is connected to the standard part through these microchannels. The identification mark includes an RF tag, a two-dimensional barcode, a one-dimensional barcode, or text.

前述實施方式的其他實施例如下:前述感測裝置包含一觀測設備,其訊號連接人工智慧辨識模組。觀測設備觀測位於標準品空間之標準品而取得一標準品數位影像,並將標準品數位影像傳送至人工智慧辨識模組進行辨識,標準品數位影像對應觀測結果。Other embodiments of the aforementioned implementation method are as follows: The aforementioned sensing device includes an observation device whose signal is connected to an artificial intelligence recognition module. The observation device observes the standard product located in the standard product space to obtain a digital image of the standard product, and transmits the digital image of the standard product to the artificial intelligence recognition module for recognition, with the digital image of the standard product corresponding to the observation result.

前述實施方式的其他實施例如下:前述人工智慧辨識模組之人工智慧辨識程序包含依據標準品數位影像進行辨識與運算而取得標準品之一狀態;及計算標準品之狀態而取得一影像調校參數。其中影像調校參數包含一光線亮度或一焦距,且對應計算參數。Other embodiments of the aforementioned implementation method are as follows: The artificial intelligence identification program of the aforementioned artificial intelligence identification module includes identifying and calculating based on the digital image of the standard product to obtain a state of the standard product; and calculating the state of the standard product to obtain an image adjustment parameter. The image adjustment parameter includes a light intensity or a focal length, and a corresponding calculation parameter.

前述實施方式的其他實施例如下:前述人工智慧辨識模組之人工智慧辨識程序更包含依據影像調校參數自動化調整觀測設備,使觀測設備具有相同的一觀測狀態。Other embodiments of the aforementioned implementation method are as follows: The artificial intelligence identification program of the aforementioned artificial intelligence identification module further includes automatically adjusting the observation equipment according to the image adjustment parameters so that the observation equipment has the same observation state.

依據本發明的方法態樣之一實施方式提供一種具有自標準基準及快速部署人工智慧辨識之微流道檢測方法,包含以下步驟:藉由一感測裝置感測一微流道晶片之一識別部之一識別記號而識別出一標誌資訊;藉由一處理器之一人工智慧辨識模組接收來自感測裝置之標誌資訊,並依據標誌資訊自動導入對應之一人工智慧辨識程序;以及藉由處理器之人工智慧辨識模組依據人工智慧辨識程序將微流道晶片之一標準部之一標準品空間的一觀測結果轉換成一計算參數,並依據計算參數自動辨識微流道晶片之一觀察部之一觀察空間而產生一實驗結果。其中微流道晶片對應感測裝置且包含複數注入孔、標準部、觀察部、複數微流道及一識別部。標準部包含一標準品且具有標準品空間,標準品設置於標準品空間,觀察部具有觀察空間。此些注入孔、標準部及觀察部之二者透過此些微流道之一者連通。According to one embodiment of the present invention, a microchannel detection method with self-standard benchmark and rapid deployment of artificial intelligence identification is provided, comprising the following steps: identifying a mark information by sensing a mark in an identification part of a microchannel chip using a sensing device; receiving the mark information from the sensing device by an artificial intelligence identification module of a processor, and automatically importing a corresponding artificial intelligence identification program according to the mark information; and converting an observation result of a standard space in a standard part of a microchannel chip into a calculation parameter by the artificial intelligence identification program using the artificial intelligence identification module of the processor, and automatically identifying an observation space in an observation part of a microchannel chip according to the calculation parameter to generate an experimental result. The microchannel chip corresponds to a sensing device and includes a plurality of injection ports, a standard section, an observation section, a plurality of microchannels, and an identification section. The standard section includes a standard sample and has a standard sample space, in which the standard sample is disposed. The observation section has an observation space. The injection ports, the standard section, and the observation section are connected through one of the microchannels.

前述實施方式的其他實施例如下:前述標準品包含一物理性物件或一化學性物件,標準品空間與觀察空間之任一者為一三維空間,三維空間為球體或方體。標準部與觀察部之一者具有透光性及低光線散射性。此些注入孔之一者與標準部透過此些微流道之此者連通。識別記號包含一射頻標籤、一二維條碼、一維條碼或一文字。Other embodiments of the aforementioned implementation method are as follows: The aforementioned standard includes a physical object or a chemical object, and either the standard space or the observation space is a three-dimensional space, which is a sphere or a cube. One of the standard part and the observation part is transparent and has low light scattering. One of these injection holes is connected to the standard part through these microchannels. The identification mark includes an RF tag, a two-dimensional barcode, a one-dimensional barcode, or text.

前述實施方式的其他實施例如下:前述具有自標準基準及快速部署人工智慧辨識之微流道檢測方法更包含:藉由感測裝置之一觀測設備觀測位於標準品空間之標準品而取得一標準品數位影像,並將標準品數位影像傳送至人工智慧辨識模組進行辨識。其中標準品數位影像對應觀測結果。Other embodiments of the aforementioned implementation method are as follows: The aforementioned microfluidic channel detection method with self-standard benchmark and rapid deployment of artificial intelligence identification further includes: observing a standard sample located in the standard sample space using an observation device of one of the sensing devices to obtain a digital image of the standard sample, and transmitting the digital image of the standard sample to an artificial intelligence identification module for identification. The digital image of the standard sample corresponds to the observation result.

前述實施方式的其他實施例如下:前述人工智慧辨識程序包含:藉由人工智慧辨識模組依據標準品數位影像進行辨識與運算而取得標準品之一狀態;及藉由人工智慧辨識模組計算標準品之狀態而取得一影像調校參數。其中影像調校參數包含一光線亮度或一焦距,且對應計算參數。Other embodiments of the aforementioned implementation method are as follows: The aforementioned artificial intelligence identification process includes: obtaining a state of the standard product by performing identification and calculation based on the digital image of the standard product using an artificial intelligence identification module; and obtaining an image adjustment parameter by calculating the state of the standard product using the artificial intelligence identification module. The image adjustment parameter includes a light intensity or a focal length, and corresponds to the calculation parameter.

前述實施方式的其他實施例如下:前述人工智慧辨識程序更包含藉由人工智慧辨識模組依據影像調校參數自動化調整觀測設備,使觀測設備具有相同的一觀測狀態。Other embodiments of the aforementioned implementation method are as follows: The aforementioned artificial intelligence identification program further includes automatically adjusting the observation equipment according to the image adjustment parameters by the artificial intelligence identification module, so that the observation equipment has the same observation state.

藉此,本發明之具有自標準基準及快速部署人工智慧辨識之微流道檢測系統及其方法可用於具有物理與化學的生物細胞、生物化學等實驗,藉由標誌資訊之辨識可自動導入對應之人工智慧辨識程序,可有效縮短檢測時間。此外,透過人工智慧辨識程序在觀察區域進行自動化辨識而快速取得檢測結果,可減少人工判讀的誤差。Therefore, the microfluidic channel detection system and method of this invention, which features self-standardized benchmarks and rapid deployment of artificial intelligence recognition, can be used in experiments involving physical and chemical processes such as biological cells and biochemistry. By identifying marker information, the corresponding artificial intelligence recognition program can be automatically imported, effectively shortening the detection time. Furthermore, by automating the identification process within the observation area through artificial intelligence recognition, detection results can be quickly obtained, reducing errors from human interpretation.

以下將參照圖式說明本發明的複數個實施例。為明確說明起見,許多實務上的細節將在以下敘述中一併說明。然而,應瞭解到,這些實務上的細節不應用以限制本發明。也就是說,在本發明部分實施例中,這些實務上的細節是非必要的。此外,為簡化圖式起見,一些習知慣用的結構與元件在圖式中將以簡單示意的方式繪示的;並且重複的元件將可能使用相同的編號表示的。Several embodiments of the present invention will be described below with reference to the drawings. For clarity, many practical details will be described in the following description. However, it should be understood that these practical details should not be used to limit the present invention. That is, these practical details are not essential in some embodiments of the present invention. Furthermore, for the sake of simplicity, some conventional structures and components will be shown in the drawings in a simple schematic manner; and repeated components may be represented by the same designation.

請參閱第1圖,第1圖係繪示本發明之第一實施例之具有自標準基準及快速部署人工智慧辨識之微流道檢測系統100之方塊示意圖。具有自標準基準及快速部署人工智慧辨識之微流道檢測系統100包含一感測裝置200、一微流道晶片300以及一處理器400。微流道晶片300對應感測裝置200且包含複數注入孔310、一標準部320、一觀察部330、複數微流道340及一識別部350。此些注入孔310、標準部320及觀察部330之二者透過此些微流道340之一者連通。標準部320包含一標準品且具有一標準品空間,標準品設置於標準品空間。觀察部330具有一觀察空間。識別部350具有一識別記號,識別記號用以供感測裝置200感測而識別出一標誌資訊。處理器400訊號連接感測裝置200與微流道晶片300,且包含一人工智慧辨識模組410。人工智慧辨識模組410接收來自感測裝置200之標誌資訊,並依據標誌資訊自動導入對應之一人工智慧辨識程序,並依據人工智慧辨識程序將標準品空間的一觀測結果轉換成一計算參數,並依據計算參數自動辨識觀察空間而產生一實驗結果。Please refer to Figure 1, which is a block diagram illustrating a microchannel detection system 100 with self-standardized benchmark and rapid deployment of artificial intelligence recognition according to a first embodiment of the present invention. The microchannel detection system 100 with self-standardized benchmark and rapid deployment of artificial intelligence recognition includes a sensing device 200, a microchannel chip 300, and a processor 400. The microchannel chip 300 corresponds to the sensing device 200 and includes a plurality of injection holes 310, a standard section 320, an observation section 330, a plurality of microchannels 340, and an identification section 350. The injection holes 310, the standard section 320, and the observation section 330 are connected through one of the microchannels 340. The standard section 320 includes a standard sample and has a standard sample space, in which the standard sample is disposed. The observation unit 330 has an observation space. The identification unit 350 has an identification mark for the sensing device 200 to detect and identify a mark information. The processor 400 is signal-connected to the sensing device 200 and the microfluidic chip 300, and includes an artificial intelligence identification module 410. The artificial intelligence identification module 410 receives the mark information from the sensing device 200, automatically imports a corresponding artificial intelligence identification program according to the mark information, converts an observation result of the standard space into a calculation parameter according to the artificial intelligence identification program, and automatically identifies the observation space and generates an experimental result according to the calculation parameter.

藉此,本發明之具有自標準基準及快速部署人工智慧辨識之微流道檢測系統100藉由標誌資訊之辨識可自動導入對應之人工智慧辨識程序,並透過人工智慧辨識程序在觀察區域進行自動化辨識而快速取得檢測結果,可有效縮短檢測時間並減少人工判讀的誤差。Therefore, the microfluidic channel detection system 100 of this invention, which has a self-standard benchmark and rapid deployment of artificial intelligence recognition, can automatically import the corresponding artificial intelligence recognition program through the identification of mark information, and quickly obtain detection results through the automatic identification of the observation area by the artificial intelligence recognition program, which can effectively shorten the detection time and reduce the error of human interpretation.

感測裝置200包含一觀測設備210、一標誌辨識模組220及電物化模組230。觀測設備210訊號連接人工智慧辨識模組410,觀測設備210觀測位於標準品空間之標準品而取得一標準品數位影像,並將標準品數位影像傳送至人工智慧辨識模組410進行辨識。標準品數位影像對應觀測結果。標誌辨識模組220對應並感測識別部350之識別記號,以識別出標誌資訊。標誌辨識模組220可透過無線射頻辨識(Radio Frequency IDentification;RFID)電磁波感應或者攝影鏡頭影像辨識完成辨識。電物化模組230可包含電物化感測元件,其可設置於微流道晶片300內,以檢測實驗主體的物理性質或化學性質,並將所述性質傳送至處理器400進行分析。實驗主體位於觀察空間中。The sensing device 200 includes an observation device 210, a mark recognition module 220, and an electrophysical module 230. The observation device 210 is signal-connected to the artificial intelligence recognition module 410. The observation device 210 observes a standard sample located in the standard sample space to obtain a digital image of the standard sample and transmits the digital image to the artificial intelligence recognition module 410 for identification. The digital image of the standard sample corresponds to the observation result. The mark recognition module 220 corresponds to and senses the identification mark of the identification unit 350 to identify the mark information. The mark recognition module 220 can complete the identification through radio frequency identification (RFID) electromagnetic wave sensing or video camera image recognition. The electrophysical module 230 may include an electrophysical sensing element disposed within the microfluidic chip 300 to detect the physical or chemical properties of the experimental subject and transmit the properties to the processor 400 for analysis. The experimental subject is located in the observation space.

標準部320之標準品包含一物理性物件(例如:螢光珠、微珠、磁珠等)或一化學性物件(例如:標準試劑)。標準品空間與觀察空間之任一者為一三維空間,三維空間為球體或方體,其為除微流道340連通孔洞外其餘皆密閉之立體空間。標準部320與觀察部330之一者具有透光性及低光線散射性。此些注入孔310之一者與標準部320透過此些微流道340之一者連通。識別記號包含一射頻標籤(如RFID標籤)、一二維條碼(如QR碼)、一維條碼或一文字。識別記號之部分可利用雷射刻畫(尺規、文字及記號)。The standard component of the standard section 320 includes a physical object (e.g., fluorescent beads, microbeads, magnetic beads, etc.) or a chemical object (e.g., a standard reagent). Either the standard component space or the observation space is a three-dimensional space, which is a sphere or cube, and is a closed three-dimensional space except for the connecting holes of the microchannels 340. One of the standard section 320 and the observation section 330 is translucent and has low light scattering. One of the injection holes 310 is connected to the standard section 320 through one of the microchannels 340. The identification mark includes an RFID tag (e.g., a two-dimensional barcode, such as a QR code), a one-dimensional barcode, or text. The identification mark can be laser-engraved (rulers, text, and symbols).

若標準品是物理性物件,注入孔310、標準部320之標準品空間及觀察部330之觀察空間彼此可透過微流道340連通。若標準品是化學性物件,注入孔310與標準部320彼此可透過微流道340連通,注入孔310與觀察部330彼此可透過微流道340連通,而標準部320與觀察部330不連通。此外,標準品空間可連通電極或金屬,做電性或磁性操作。標準品之材料會預先置入於標準品空間中。If the standard is a physical object, the injection port 310, the standard space of the standard section 320, and the observation space of the observation section 330 can be connected to each other through the microchannel 340. If the standard is a chemical object, the injection port 310 and the standard section 320 can be connected to each other through the microchannel 340, and the injection port 310 and the observation section 330 can be connected to each other through the microchannel 340, while the standard section 320 and the observation section 330 are not connected. Furthermore, the standard space can be connected to an electrode or metal for electrical or magnetic operation. The material of the standard is pre-placed in the standard space.

處理器400可為中央處理器(Central Processing Unit;CPU)、圖形處理器(Graphics Processing Unit;GPU)、電腦、行動裝置處理器、雲端處理器或其他高效能的運算處理器,但本發明不以此為限。The processor 400 may be a central processing unit (CPU), a graphics processing unit (GPU), a computer, a mobile device processor, a cloud processor, or other high-performance computing processor, but the present invention is not limited thereto.

請一併參閱第1圖與第2圖,其中第2圖係繪示本發明之第二實施例之微流道晶片300a之標準品SP為物理性物件之示意圖。微流道晶片300a包含第一注入孔3101、第二注入孔3102、第三注入孔3103、一標準部320a、一觀察部330a及複數微流道340a。標準部320a包含一標準品SP且具有一標準品空間SS,標準品SP設置於標準品空間SS。觀察部330a具有一觀察空間OS。第一注入孔3101、第二注入孔3102、第三注入孔3103、標準部320a之標準品空間SS及觀察部330a之觀察空間OS彼此透過此些微流道340a連通。Please refer to Figures 1 and 2 together, where Figure 2 is a schematic diagram illustrating the standard sample SP of the microchannel chip 300a of the second embodiment of the present invention as a physical object. The microchannel chip 300a includes a first injection port 3101, a second injection port 3102, a third injection port 3103, a standard section 320a, an observation section 330a, and a plurality of microchannels 340a. The standard section 320a includes a standard sample SP and has a standard sample space SS, in which the standard sample SP is disposed. The observation section 330a has an observation space OS. The first injection port 3101, the second injection port 3102, the third injection port 3103, the standard sample space SS of the standard section 320a, and the observation space OS of the observation section 330a are interconnected through these microchannels 340a.

請一併參閱第1圖與第3圖,其中第3圖係繪示本發明之第三實施例之微流道晶片300b之標準品為化學性物件之示意圖。微流道晶片300b包含第一注入孔3101、第二注入孔3102、第三注入孔3103、一標準部320b、一觀察部330b及複數微流道340b。標準部320b包含一標準品(標準試劑)且具有一標準品空間SS,標準試劑可由微流道340b注入至標準品空間SS。觀察部330b具有一觀察空間OS。第一注入孔3101、第三注入孔3103及標準部320b彼此透過微流道340b連通,第二注入孔3102與觀察部330b彼此透過微流道340b連通,而標準部320b與觀察部330b不連通(如透過逆止閥)。Please refer to Figures 1 and 3 together, where Figure 3 is a schematic diagram illustrating that the standard of the microchannel chip 300b of the third embodiment of the present invention is a chemical object. The microchannel chip 300b includes a first injection port 3101, a second injection port 3102, a third injection port 3103, a standard section 320b, an observation section 330b, and a plurality of microchannels 340b. The standard section 320b includes a standard (standard reagent) and has a standard space SS, into which the standard reagent can be injected through the microchannels 340b. The observation section 330b has an observation space OS. The first injection port 3101, the third injection port 3103 and the standard section 320b are connected to each other through the microchannel 340b, the second injection port 3102 and the observation section 330b are connected to each other through the microchannel 340b, while the standard section 320b and the observation section 330b are not connected (e.g. through a check valve).

請一併參閱第1圖與第4圖,其中第4圖係繪示本發明之第四實施例之微流道晶片300c之示意圖。微流道晶片300c屬於插銷型態,其可為拋棄式或重複利用式。微流道晶片300c包含第一注入孔3101、第二注入孔3102、第三注入孔3103、一標準部320c、一觀察部330c及複數微流道340c。第一注入孔3101、第二注入孔3102、第三注入孔3103及標準部320c彼此透過微流道340c連通,標準部320c與觀察部330c彼此透過微流道340c連通。Please refer to Figures 1 and 4 together, where Figure 4 is a schematic diagram illustrating the microchannel chip 300c of the fourth embodiment of the present invention. The microchannel chip 300c is of the plug type and can be disposable or reusable. The microchannel chip 300c includes a first injection port 3101, a second injection port 3102, a third injection port 3103, a standard section 320c, an observation section 330c, and a plurality of microchannels 340c. The first injection port 3101, the second injection port 3102, the third injection port 3103, and the standard section 320c are connected to each other through the microchannels 340c, and the standard section 320c and the observation section 330c are connected to each other through the microchannels 340c.

請一併參閱第1圖與第5圖,其中第5圖係繪示本發明之第五實施例之微流道晶片300d之示意圖。微流道晶片300d屬於插銷型態,其可為拋棄式或重複利用式。微流道晶片300d包含第一注入孔3101、第二注入孔3102、第三注入孔3103、一標準部320d、一觀察部330d及複數微流道340d。第一注入孔3101、第二注入孔3102、第三注入孔3103及觀察部330d彼此透過微流道340d連通,標準部320d與觀察部330d彼此透過微流道340d連通。Please refer to Figures 1 and 5 together, where Figure 5 is a schematic diagram illustrating the microchannel chip 300d of the fifth embodiment of the present invention. The microchannel chip 300d is of the plug type and can be disposable or reusable. The microchannel chip 300d includes a first injection port 3101, a second injection port 3102, a third injection port 3103, a standard section 320d, an observation section 330d, and a plurality of microchannels 340d. The first injection port 3101, the second injection port 3102, the third injection port 3103, and the observation section 330d are interconnected through the microchannels 340d, and the standard section 320d and the observation section 330d are interconnected through the microchannels 340d.

請一併參閱第1圖與第6圖,其中第6圖係繪示本發明之第六實施例之微流道晶片300e之示意圖。微流道晶片300e包含一標準部320e、複數觀察部330e及複數微流道340e。各觀察部330e與標準部320e彼此透過微流道340e連通。微流道340e鄰近觀察部330e之處設有逆止閥342,且具有一微流道逆止區344、一緩衝區346及一微流道減壓區348。逆止閥342設置於微流道逆止區344與觀察部330e之間,可用以防止觀察部330e與標準部320e相互干擾。緩衝區346位於微流道逆止區344與微流道減壓區348之間。Please refer to Figures 1 and 6 together, where Figure 6 is a schematic diagram illustrating the microchannel chip 300e of the sixth embodiment of the present invention. The microchannel chip 300e includes a standard section 320e, a plurality of observation sections 330e, and a plurality of microchannels 340e. Each observation section 330e and the standard section 320e are connected to each other through the microchannels 340e. A check valve 342 is provided near the observation section 330e in each microchannel 340e, and it has a microchannel check area 344, a buffer area 346, and a microchannel pressure reduction area 348. The check valve 342 is disposed between the microchannel check area 344 and the observation section 330e, and can be used to prevent the observation section 330e and the standard section 320e from interfering with each other. The buffer zone 346 is located between the microchannel check zone 344 and the microchannel depressurization zone 348.

請一併參閱第1圖與第7圖,其中第7圖係繪示本發明之第七實施例之具有自標準基準及快速部署人工智慧辨識之微流道檢測方法500之流程示意圖。具有自標準基準及快速部署人工智慧辨識之微流道檢測方法500包含進行步驟S02、S04、S06、S08、S10、S12、S14、S16、S18。Please refer to Figures 1 and 7 together. Figure 7 is a flowchart illustrating the microchannel detection method 500 with self-standard benchmark and rapid deployment of artificial intelligence recognition according to the seventh embodiment of the present invention. The microchannel detection method 500 with self-standard benchmark and rapid deployment of artificial intelligence recognition includes steps S02, S04, S06, S08, S10, S12, S14, S16, and S18.

步驟S02包含置入微流道晶片300。步驟S04包含藉由人工智慧A掃描與辨識標誌資訊,亦即透過接觸或非接觸方式,取得RFID標籤資訊、QR碼標誌辨識結果或雷射刻畫標誌辨識結果。步驟S06包含載入對應人工智慧B待機,亦即取得標誌資訊所對應之人工智慧辨識模組410(人工智慧B)資訊,其中取得方式可直接於微流道晶片300取得或者透過位於觀測站台或設備(如電腦或顯微鏡)之網路遠端取得。步驟S02、S04、S06可視為一步驟SA,步驟SA係執行人工智慧A。Step S02 includes embedding the microfluidic chip 300. Step S04 includes scanning and identifying tag information using AI A, i.e., obtaining RFID tag information, QR code identification results, or laser-engraved tag identification results through contact or non-contact methods. Step S06 includes loading the corresponding AI B standby, i.e., obtaining the AI identification module 410 (AI B) information corresponding to the tag information, which can be obtained directly from the microfluidic chip 300 or remotely via a network located at an observation station or device (such as a computer or microscope). Steps S02, S04, and S06 can be considered as one step SA, where step SA executes AI A.

步驟S08包含藉由觀測設備210載入即時影像。步驟S10包含檢索微流道晶片300之標準品空間。步驟S12包含藉由人工智慧B自動化參數調校。步驟S14包含依據參數自動化調整觀測設備210。步驟S16包含調整後觀測設備210載入即時影像。步驟S08、S10、S12、S14、S16可視為一步驟SB,步驟SB係執行人工智慧B。Step S08 includes loading real-time images using observation device 210. Step S10 includes retrieving the standard space of the microfluidic chip 300. Step S12 includes automated parameter adjustment using AI B. Step S14 includes automated adjustment of observation device 210 based on parameters. Step S16 includes loading real-time images into the adjusted observation device 210. Steps S08, S10, S12, S14, and S16 can be considered as one step SB, where step SB executes AI B.

由上述可知,步驟SB包含藉由感測裝置200之觀測設備210觀測位於標準品空間之標準品而取得一標準品數位影像,並將標準品數位影像傳送至人工智慧辨識模組410進行辨識。標準品數位影像對應觀測結果。步驟SB更包含執行人工智慧辨識程序,人工智慧辨識程序包含藉由人工智慧辨識模組410依據標準品數位影像進行辨識與運算而取得標準品之一狀態,並藉由人工智慧辨識模組410計算標準品之狀態而取得一影像調校參數。影像調校參數包含一光線亮度或一焦距,且對應計算參數。此外,人工智慧辨識程序更包含藉由人工智慧辨識模組410依據影像調校參數自動化調整觀測設備210,使觀測設備210在觀測過程中具有相同的一觀測狀態,並自動化計算與調整觀測影像(如影像前處理之亮度、對比或放大縮小等),且調校參數結果可做為觀測設備210及周邊聯繫設備自動化調整。As described above, step SB includes acquiring a digital image of the standard sample by observing the standard sample located in the standard sample space using the observation device 210 of the sensing device 200, and transmitting the digital image to the artificial intelligence recognition module 410 for recognition. The digital image of the standard sample corresponds to the observation result. Step SB further includes executing an artificial intelligence recognition program, which includes obtaining a state of the standard sample by recognizing and calculating based on the digital image of the standard sample using the artificial intelligence recognition module 410, and obtaining an image adjustment parameter by calculating the state of the standard sample using the artificial intelligence recognition module 410. The image adjustment parameter includes a brightness or a focal length, and corresponds to the calculation parameter. In addition, the artificial intelligence recognition program includes an artificial intelligence recognition module 410 that automatically adjusts the observation device 210 according to the image adjustment parameters, so that the observation device 210 has the same observation state during the observation process, and automatically calculates and adjusts the observed image (such as brightness, contrast or zoom in/out of the image preprocessing), and the adjustment parameter results can be used for the automatic adjustment of the observation device 210 and peripheral connected devices.

步驟S18包含藉由人工智慧C自動化影像辨識,亦即利用來自人工智慧A辨識結果選取對應之人工智慧C,不同之人工智慧C具有特定的辨識目標(如特定細胞、細菌、病毒或抗體)。步驟S18可視為一步驟SC,步驟SC係執行人工智慧C。Step S18 involves automated image recognition using artificial intelligence (AI) C, that is, selecting the corresponding AI C based on the recognition results from AI A. Different AI Cs have specific recognition targets (such as specific cells, bacteria, viruses, or antibodies). Step S18 can be regarded as a step SC, which executes AI C.

上述人工智慧A、B、C可透過人工智慧軟體模組或人工智慧硬體模組實現,其中人工智慧軟體模組可以由遠端伺服器(如雲端)透過網路下載到近端的邊緣運算設備(連接觀測設備210),進行觀測設備210之影像辨識運作。人工智慧硬體模組可以直接樞合在近端的邊緣運算設備(連接觀測設備210)進行運算。無論是透過軟體模組或硬體模組,利用自動化均可實現快速部署人工智慧辨識之目標。上述人工智慧辨識程序可為類神經網路、深度學習,但本發明不以上述為限。The aforementioned artificial intelligences A, B, and C can be implemented through artificial intelligence software modules or artificial intelligence hardware modules. The artificial intelligence software module can be downloaded from a remote server (such as the cloud) via a network to a near-end edge computing device (connected to observation device 210) to perform image recognition operations on observation device 210. The artificial intelligence hardware module can be directly integrated into the near-end edge computing device (connected to observation device 210) for computation. Whether through software or hardware modules, automation can achieve the goal of rapid deployment of artificial intelligence recognition. The aforementioned artificial intelligence recognition program can be a neural network or deep learning-based system, but this invention is not limited to these.

由上述實施方式可知,本發明具有下列優點:其一,可用於具有物理與化學的生物細胞、生物化學等實驗,藉由標誌資訊之辨識可自動導入對應之人工智慧辨識程序,可有效縮短檢測時間。其二,透過人工智慧辨識程序在觀察區域(即觀察空間)進行自動化辨識而快速取得檢測結果,可減少人工判讀的誤差。As can be seen from the above implementation method, the present invention has the following advantages: First, it can be used in experiments involving physical and chemical biological cells, biochemistry, etc., and can automatically import corresponding artificial intelligence identification programs through the identification of label information, which can effectively shorten the detection time. Second, by using artificial intelligence identification programs to automatically identify in the observation area (i.e., the observation space) to quickly obtain detection results, the error of human interpretation can be reduced.

雖然本發明已以實施方式揭露如上,然其並非用以限定本發明,任何熟習此技藝者,在不脫離本發明之精神和範圍內,當可作各種之更動與潤飾,因此發明之保護範圍當視後附之申請專利範圍所界定者為準。Although the invention has been disclosed above by way of implementation, it is not intended to limit the invention. Anyone skilled in the art may make various modifications and alterations without departing from the spirit and scope of the invention. Therefore, the scope of protection of the invention shall be determined by the scope of the appended patent application.

100:具有自標準基準及快速部署人工智慧辨識之微流道檢測系統 200:感測裝置 210:觀測設備 220:標誌辨識模組 230:電物化模組 300,300a,300b,300c,300d,300e:微流道晶片 310:注入孔 3101:第一注入孔 3102:第二注入孔 3103:第三注入孔 320,320a,320b,320c,320d,320e:標準部 330,330a,330b,330c,330d,330e:觀察部 340,340a,340b,340c,340d,340e:微流道 342:逆止閥 344:微流道逆止區 346:緩衝區 348:微流道減壓區 350:識別部 400:處理器 410:人工智慧辨識模組 500:具有自標準基準及快速部署人工智慧辨識之微流道檢測方法 OS:觀察空間 S02,S04,S06,S08,S10,S12,S14,S16,S18,SA,SB,SC:步驟 SP:標準品 SS:標準品空間100: Microfluidic channel inspection system with self-standard benchmark and rapid deployment of AI recognition 200: Sensing device 210: Observation equipment 220: Mark recognition module 230: Electrophysical module 300, 300a, 300b, 300c, 300d, 300e: Microfluidic chip 310: Injection port 3101: First injection port 3102: Second injection port 3103: Third injection port 320, 320a, 320b, 320c, 320d, 320e: Standard section 330, 330a, 330b, 330c, 330d, 330e: Observation section 340, 340a, 340b, 340c, 340d, 340e: Microchannel 342: Check Valve 344: Microchannel Check Zone 346: Buffer Zone 348: Microchannel Pressure Reduction Zone 350: Identification Unit 400: Processor 410: Artificial Intelligence Identification Module 500: Microchannel Detection Method with Self-Standardized Baseline and Rapid Deployment of Artificial Intelligence Identification OS: Observation Space S02, S04, S06, S08, S10, S12, S14, S16, S18, SA, SB, SC: Steps SP: Standard Sample SS: Standard Sample Space

第1圖係繪示本發明之第一實施例之具有自標準基準及快速部署人工智慧辨識之微流道檢測系統之方塊示意圖; 第2圖係繪示本發明之第二實施例之微流道晶片之標準品為物理性物件之示意圖; 第3圖係繪示本發明之第三實施例之微流道晶片之標準品為化學性物件之示意圖; 第4圖係繪示本發明之第四實施例之微流道晶片之示意圖; 第5圖係繪示本發明之第五實施例之微流道晶片之示意圖; 第6圖係繪示本發明之第六實施例之微流道晶片之示意圖;以及 第7圖係繪示本發明之第七實施例之具有自標準基準及快速部署人工智慧辨識之微流道檢測方法之流程示意圖。 Figure 1 is a block diagram illustrating a microchannel inspection system with self-standardization and rapid deployment of AI recognition according to the first embodiment of the present invention; Figure 2 is a schematic diagram illustrating that the standard for the microchannel chip in the second embodiment of the present invention is a physical object; Figure 3 is a schematic diagram illustrating that the standard for the microchannel chip in the third embodiment of the present invention is a chemical object; Figure 4 is a schematic diagram illustrating a microchannel chip in the fourth embodiment of the present invention; Figure 5 is a schematic diagram illustrating a microchannel chip in the fifth embodiment of the present invention; Figure 6 is a schematic diagram illustrating a microchannel chip in the sixth embodiment of the present invention; and Figure 7 is a flowchart illustrating a microchannel inspection method with self-standardization and rapid deployment of AI recognition according to the seventh embodiment of the present invention.

100:具有自標準基準及快速部署人工智慧辨識之微流道檢測系統 100: A microfluidic channel inspection system with self-standardized benchmarks and rapid deployment of AI-based identification.

200:感測裝置 200: Sensing Device

210:觀測設備 210: Observational Equipment

220:標誌辨識模組 220: Logo Recognition Module

230:電物化模組 230: Electromechanical Module

300:微流道晶片 300: Microchannel chip

310:注入孔 310: Injection Hole

320:標準部 320:Standards Department

330:觀察部 330: Observation Department

340:微流道 340: Microchannel

350:識別部 350:Identification Department

400:處理器 400: Processor

410:人工智慧辨識模組 410: Artificial Intelligence Recognition Module

Claims (10)

一種具有自標準基準及快速部署人工智慧辨識之微流道檢測系統,包含: 一感測裝置; 一微流道晶片,對應該感測裝置且包含: 複數注入孔; 一標準部,包含一標準品且具有一標準品空間,該標準品設置於該標準品空間; 一觀察部,具有一觀察空間; 複數微流道,其中該些注入孔、該標準部及該觀察部之二者透過該些微流道之一者連通;及 一識別部,具有一識別記號,該識別記號用以供該感測裝置感測而識別出一標誌資訊;以及 一處理器,訊號連接該感測裝置與該微流道晶片,且包含一人工智慧辨識模組,該人工智慧辨識模組接收來自該感測裝置之該標誌資訊,並依據該標誌資訊自動導入對應之一人工智慧辨識程序,並依據該人工智慧辨識程序將該標準品空間的一觀測結果轉換成一計算參數,並依據該計算參數自動辨識該觀察空間而產生一實驗結果。 A microchannel detection system with self-standardized benchmarks and rapid deployment of AI recognition, comprising: a sensing device; a microchannel chip corresponding to the sensing device and including: a plurality of injection holes; a standard section including a standard and having a standard space, the standard being disposed in the standard space; an observation section having an observation space; a plurality of microchannels, wherein the injection holes, the standard section, and the observation section are connected through one of the microchannels; and an identification section having an identification mark, the identification mark being used by the sensing device to sense and identify a mark information; and A processor, signal-connected to the sensing device and the microfluidic chip, includes an artificial intelligence (AI) recognition module. This AI recognition module receives the marker information from the sensing device, automatically imports a corresponding AI recognition program based on the marker information, converts an observation result of the standard space into a calculation parameter based on the AI recognition program, and automatically identifies the observation space based on the calculation parameter to generate an experimental result. 如請求項1所述之具有自標準基準及快速部署人工智慧辨識之微流道檢測系統,其中該標準品包含一物理性物件或一化學性物件,該標準品空間與該觀察空間之任一者為一三維空間,該三維空間為球體或方體,該標準部與該觀察部之一者具有透光性及低光線散射性,該些注入孔之一者與該標準部透過該些微流道之該者連通,該識別記號包含一射頻標籤、一二維條碼、一維條碼或一文字。The microchannel detection system with self-standardized benchmark and rapid deployment of artificial intelligence identification as described in claim 1, wherein the standard comprises a physical object or a chemical object, and either the standard space or the observation space is a three-dimensional space, the three-dimensional space being a sphere or a cube, one of the standard part and the observation part having light transmittance and low light scattering, one of the injection holes being connected to the standard part through the microchannels, and the identification mark comprising an RF tag, a two-dimensional barcode, a one-dimensional barcode, or text. 如請求項1所述之具有自標準基準及快速部署人工智慧辨識之微流道檢測系統,其中該感測裝置包含: 一觀測設備,訊號連接該人工智慧辨識模組,該觀測設備觀測位於該標準品空間之該標準品而取得一標準品數位影像,並將該標準品數位影像傳送至該人工智慧辨識模組進行辨識,該標準品數位影像對應該觀測結果。 The microfluidic channel detection system with self-standardized benchmark and rapid deployment of artificial intelligence identification as described in claim 1, wherein the sensing device comprises: an observation device, signal-connected to the artificial intelligence identification module, the observation device observing the standard sample located in the standard sample space to acquire a digital image of the standard sample, and transmitting the digital image of the standard sample to the artificial intelligence identification module for identification, the digital image of the standard sample corresponding to the observation result. 如請求項3所述之具有自標準基準及快速部署人工智慧辨識之微流道檢測系統,其中該人工智慧辨識模組之該人工智慧辨識程序包含: 依據該標準品數位影像進行辨識與運算而取得該標準品之一狀態;及 計算該標準品之該狀態而取得一影像調校參數; 其中,該影像調校參數包含一光線亮度或一焦距,且對應該計算參數。 The microfluidic channel detection system with self-standard benchmark and rapid deployment of artificial intelligence recognition as described in claim 3, wherein the artificial intelligence recognition module's artificial intelligence recognition program includes: identifying and calculating a state of the standard product based on a digital image of the standard product; and calculating the state of the standard product to obtain an image adjustment parameter; wherein, the image adjustment parameter includes a light intensity or a focal length, and corresponds to the calculated parameter. 如請求項4所述之具有自標準基準及快速部署人工智慧辨識之微流道檢測系統,其中該人工智慧辨識模組之該人工智慧辨識程序更包含: 依據該影像調校參數自動化調整該觀測設備,使該觀測設備具有相同的一觀測狀態。 The microfluidic channel detection system with self-standard benchmark and rapid deployment of artificial intelligence recognition as described in claim 4, wherein the artificial intelligence recognition module's artificial intelligence recognition program further includes: Automatically adjusting the observation equipment according to the image calibration parameters to ensure that the observation equipment has the same observation state. 一種具有自標準基準及快速部署人工智慧辨識之微流道檢測方法,包含以下步驟: 藉由一感測裝置感測一微流道晶片之一識別部之一識別記號而識別出一標誌資訊; 藉由一處理器之一人工智慧辨識模組接收來自該感測裝置之該標誌資訊,並依據該標誌資訊自動導入對應之一人工智慧辨識程序;以及 藉由該處理器之該人工智慧辨識模組依據該人工智慧辨識程序將該微流道晶片之一標準部之一標準品空間的一觀測結果轉換成一計算參數,並依據該計算參數自動辨識該微流道晶片之一觀察部之一觀察空間而產生一實驗結果; 其中,該微流道晶片對應該感測裝置且包含複數注入孔、該標準部、該觀察部、複數微流道及一識別部,該標準部包含一標準品且具有該標準品空間,該標準品設置於該標準品空間,該觀察部具有該觀察空間,該些注入孔、該標準部及該觀察部之二者透過該些微流道之一者連通。 A microchannel detection method with self-standardized benchmarks and rapid deployment of artificial intelligence (AI) recognition includes the following steps: Sensing an identification mark on an identification section of a microchannel chip using a sensing device to identify a mark information; Receiving the mark information from the sensing device using an AI recognition module of a processor, and automatically importing a corresponding AI recognition program based on the mark information; The AI recognition module of the processor converts an observation result of a standard space in a standard section of the microchannel chip into a calculation parameter according to the AI recognition program, and automatically identifies an observation space in an observation section of the microchannel chip based on the calculation parameter to generate an experimental result; The microchannel chip corresponds to the sensing device and includes a plurality of injection ports, a standard section, an observation section, a plurality of microchannels, and an identification section. The standard section includes a standard sample and has a standard sample space disposed therein. The observation section has an observation space. The injection ports, the standard section, and the observation section are connected through one of the microchannels. 如請求項6所述之具有自標準基準及快速部署人工智慧辨識之微流道檢測方法,其中該標準品包含一物理性物件或一化學性物件,該標準品空間與該觀察空間之任一者為一三維空間,該三維空間為球體或方體,該標準部與該觀察部之一者具有透光性及低光線散射性,該些注入孔之一者與該標準部透過該些微流道之該者連通,該識別記號包含一射頻標籤、一二維條碼、一維條碼或一文字。The microchannel detection method with self-standard benchmark and rapid deployment of artificial intelligence identification as described in claim 6, wherein the standard comprises a physical object or a chemical object, either the standard space or the observation space is a three-dimensional space, the three-dimensional space being a sphere or a cube, one of the standard part and the observation part having light transmittance and low light scattering, one of the injection holes being connected to the standard part through the microchannels, and the identification mark comprising an RF tag, a two-dimensional barcode, a one-dimensional barcode, or text. 如請求項6所述之具有自標準基準及快速部署人工智慧辨識之微流道檢測方法,更包含: 藉由該感測裝置之一觀測設備觀測位於該標準品空間之該標準品而取得一標準品數位影像,並將該標準品數位影像傳送至該人工智慧辨識模組進行辨識; 其中,該標準品數位影像對應該觀測結果。 The microfluidic channel detection method with self-standard benchmark and rapid deployment of artificial intelligence identification as described in claim 6 further comprises: observing the standard sample located in the standard sample space using an observation device of the sensing device to obtain a digital image of the standard sample, and transmitting the digital image of the standard sample to the artificial intelligence identification module for identification; wherein, the digital image of the standard sample corresponds to the observation result. 如請求項8所述之具有自標準基準及快速部署人工智慧辨識之微流道檢測方法,其中該人工智慧辨識程序包含: 藉由該人工智慧辨識模組依據該標準品數位影像進行辨識與運算而取得該標準品之一狀態;及 藉由該人工智慧辨識模組計算該標準品之該狀態而取得一影像調校參數; 其中,該影像調校參數包含一光線亮度或一焦距,且對應該計算參數。 The microfluidic channel detection method with self-standard benchmark and rapid deployment of artificial intelligence identification as described in claim 8, wherein the artificial intelligence identification process includes: obtaining a state of the standard product by performing identification and calculation based on a digital image of the standard product using the artificial intelligence identification module; and obtaining an image adjustment parameter by calculating the state of the standard product using the artificial intelligence identification module; wherein, the image adjustment parameter includes a light intensity or a focal length, and corresponds to the calculation parameter. 如請求項9所述之具有自標準基準及快速部署人工智慧辨識之微流道檢測方法,其中該人工智慧辨識程序更包含: 藉由該人工智慧辨識模組依據該影像調校參數自動化調整該觀測設備,使該觀測設備具有相同的一觀測狀態。 The microchannel detection method with self-standard benchmark and rapid deployment of artificial intelligence identification as described in claim 9, wherein the artificial intelligence identification program further includes: automatically adjusting the observation equipment according to the image calibration parameters by the artificial intelligence identification module, so that the observation equipment has the same observation state.
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