US12023664B2 - Method and kit for preservation of adipose tissue grafts - Google Patents

Method and kit for preservation of adipose tissue grafts Download PDF

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US12023664B2
US12023664B2 US16/642,585 US201816642585A US12023664B2 US 12023664 B2 US12023664 B2 US 12023664B2 US 201816642585 A US201816642585 A US 201816642585A US 12023664 B2 US12023664 B2 US 12023664B2
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tissue
vessel
fat
barrel portion
kit
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US20200346208A1 (en
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J. Peter Rubin
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University of Pittsburgh
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/05—Means for pre-treatment of biological substances by centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/22—Means for packing or storing viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/021—Adjust spacings in an array of wells, pipettes or holders, format transfer between arrays of different size or geometry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/021—Identification, e.g. bar codes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/50—Cryostats
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

Definitions

  • FIG. 1 depicts schematically, in partial cut-away view showing the filter, one aspect of a fat collection and storage device as described herein.
  • FIG. 3 C depicts schematically, in part, along with FIG. 3 A , one aspect of a method of preserving a tissue graft, such as a fat graft as described herein.
  • FIGS. 4 A and 4 B show results of analysis of ASCs (adipose-derived stem cells, FIG. 4 A ) and adipocytes ( FIG. 4 B ) for viability using trypan blue stain, as described in Example 3.
  • FIGS. 5 A and 5 B show results of analysis of ASCs ( FIG. 5 A ) and adipocytes ( FIG. 5 B ) viability using trypan blue stain, as described in Example 4.
  • the terms “right”, “left”, “top”, “bottom”, and derivatives thereof shall relate to the invention as it is oriented in the drawing figures. However, it is to be understood that the invention can assume various alternative orientations and, accordingly, such terms are not to be considered as limiting. Also, it is to be understood that the invention can assume various alternative variations and stage sequences, except where expressly specified to the contrary. It is also to be understood that the specific devices and processes illustrated in the attached drawings, and described in the following specification, are examples. Hence, specific dimensions and other physical characteristics related to the embodiments disclosed herein are not to be considered as limiting.
  • distal and proximal refer to directions with respect to the devices described herein, with “distal” referring to a direction away from a user of a device, such as the direction approaching the sharp needle tip of a hypodermic syringe (the distal end of the needle), and “proximal” referring to a direction toward a user of a device, such as the direction approaching the end of a plunger of a hypodermic syringe in the opposite direction from the sharp needle tip, at the proximal end of the syringe.
  • distal and proximal correspond to the relative position, orientation, and/or direction of element(s) of the devices described herein with respect to the end use of the device in typical use as a percutaneous device, or to other external reference points
  • those descriptors are provided only to describe the relative position, orientation, and/or direction of element(s) of the devices described herein with regard to the device as a whole, and to elements thereof, and, unless otherwise indicated, do not require or infer that the elements are located, positioned, oriented, or in any physical relationship with an end user at any given time.
  • Figures are schematic in nature unless otherwise identified, and are not drawn to scale, but are drawn in a manner to best depict the relationship between the various elements of the device drawn in the figure.
  • “Syringe,” as used herein refers to a medical or hypodermic syringe, as are broadly-known in the medical arts, and can be manufactured from glass, plastic, ceramics, and/or any acceptable material. The structure and function of a syringe, and its components are broadly-known.
  • a “cannula” is a hollow tube having a blunt end, while a “needle” for purposes of the devices and kits described herein, refers to a hollow tub having a sharp or pointed end for ease of piercing tissue, e.g., skin, blood vessels, or other tissue. For aspiration of fat, a cannula may be preferred so as to lessen the chance of puncture wounds.
  • cryoprotectant such as 10% v/v DMSO
  • effective or optimized amounts of cryoprotectants are readily ascertained.
  • slow cooling and thawing of the fat graft may be desirable in obtaining good viability of the harvested tissue.
  • Example 2 below for cryopreservation of fat cells.
  • the standard technique for harvesting and grafting employs a hollow bore harvest cannula attached to a 10 cc Luer lock syringe.
  • the surgeon introduces the cannula through a small incision and pulls back on the plunger to generate negative pressure.
  • small particles of fat are pulled into the aperture of the harvesting cannula, avulsed from the surrounding tissue with cannula movement, and drawn into the syringe barrel.
  • the fat graft material is separated from the other fractions by gentle centrifugation in a simple table-top blood centrifuge.
  • This device is commonly adapted for fat grafting and present in most operating rooms for this purpose.
  • the concentrated fat graft can be injected into the recipient tissues from the 10 cc syringe, or transferred to smaller syringes for more precise injection.
  • a hollow cannula very similar to the harvest cannula is attached to the syringe barrel.
  • All of the common fat grafting instruments are designed to attach to Luer connectors and this fact is considered in the design of one aspect of the device described herein.
  • surgeons are used to performing the centrifuge step in the operating room with the fat directly in the 10 cc syringe used for harvesting.
  • the syringe plungers are simply removed and the 10 cc syringe barrel fits into the sterilized tube of the centrifuge.
  • This point is directly relevant to the device design, because, in aspects, the device described has the same diameter and basic length of a standard, e.g., a 10 cc syringe barrel. Therefore, the fat tissue storage device can fit into the centrifuge to facilitate post-thaw washing steps without having to change containers.
  • the storage device is made to work seamlessly with the current equipment and methods commonly used by surgeons performing fat grafting.
  • a major deficiency of autologous fat grating is the fact that there is resorption of the fat graft during the initial healing, with approximately 60% of the volume of the graft remaining after healing. Therefore, multiple treatments are often necessary. Since the donor harvest procedure to obtain the fat graft is a procedure that requires an operating room setting, and special equipment to process the fat tissue, it would significantly reduce cost, risk, discomfort, and improve care if fat tissue could be stored at the time of surgery for later use. The tissue can be stored on site in small aliquots that can be injected into the site of injury in an office setting for refinement of the results. Because the tissue is stored on site, it would enable multiple treatments with minimal additional cost to the original fat harvest and original fat processing.
  • the multi-function vessels are the same diameter as a standard 10 cc syringe and nearly the same length, enabling them to be used in the same table-top centrifuge already employed for fat grafting and commonly found in operating rooms.
  • a storage vessel is provided.
  • a cylindrical device 10 is depicted.
  • the device 10 and its components are manufactured from suitable materials to withstand cell and tissue cryopreservation conditions, ranging from physiological temperature (37° C.) to storage under cryopreservation conditions, e.g., to ⁇ 20° C., ⁇ 80° C., or ⁇ 196° C. (liquid nitrogen), such as suitable polycarbonates or polypropylenes, as are known in the cryopreservation and centrifugation arts, and suitable metallic screens or filters.
  • the device 10 is dimensioned to fit into a typical clinical laboratory centrifuge able to centrifuge devices (e.g. tubes, bottles, or syringes), that is, in one aspect, it is dimensioned, e.g., as a typical 5 mL to 100 mL, e.g., 5 mL, 10 mL, 15 mL, 20 mL, 30 mL, or 60 mL medical syringe, a typical 5 mL to 50 mL, centrifuge tube (e.g., conical centrifuge tube), or a typical blood collection tube, that is, having an outside diameter of from 5 mm to 25 mm, e.g., from 12 mm to 25 mm, and a length (barrel length, excluding connectors) of from 70 mm to 110 mm.
  • a typical 5 mL to 100 mL e.g., 5 mL, 10 mL, 15 mL, 20 mL, 30
  • a storage vessel for use with a 10 mL syringe may have an outside diameter ranging from 16.5 mm to 18 mm, and a barrel length ranging from 80 mm to 90 mm.
  • the ratio of the length of the barrel to the outside diameter of the barrel is at least 4:1, e.g., from 4:1 to 6:1.
  • the device 10 is custom sized, but fits into a rotor, either with a custom or standard rotor or a custom or standard rotor insert, of a centrifuge, and its volume may range from 3 mL to 100 mL, or greater.
  • the device 10 comprises a vessel, depicted as a cylindrical barrel 20 that has a wall and an internal chamber (that is, an internal void, or a lumen).
  • the vessel is depicted as a barrel, but may have any suitable shape, though a cylindrical barrel shape may be preferred.
  • the device 10 also comprises a first end having a first fluid connector 22 comprising an opening 23 extending through the first fluid connector 22 and into the lumen of the barrel 20 , and a second end having a second fluid connector 24 comprising an opening 25 extending through the second fluid connector 24 and into to the lumen of the barrel 20 .
  • the first fluid connector 22 , the barrel 20 , and the second fluid connector 24 together form a closed fluid path between the opening of the first fluid connector 22 and the opening of the second fluid connector 24 .
  • a filter 30 is placed within the barrel 20 , adjacent to the first fluid connector 22 , and is configured to filter liquids passing through the closed fluid path between the opening of the first fluid connector 22 and the opening of the second fluid connector 24 . That is, the filter 30 spans a complete cross-section of the lumen of the barrel, so that fluid passing along the closed fluid path between the opening of the first fluid connector 22 and the opening of the second fluid connector 24 , passes through the filter 30 .
  • the device is designed and manufactured to withstand a G-force (a multiple of 1 g, the gravitational force at the earth's surface) of at least 500 g, e.g., at least 1000 g, 1200 g, or 1500 g, so as to withstand centrifugation conditions typical for washing and pelleting of viable fat grafts.
  • a G-force a multiple of 1 g, the gravitational force at the earth's surface
  • the G-force typically ranging between 400 g and 1500 g, with typical spin durations of from 1 to 5 minutes, e.g., as shown below, 1200 g for three minutes.
  • the fitting 29 is threaded, and the inside surface of the first barrel portion 26 is threaded or tapped, such that the barrel portions screw together and can be twisted relative to each other to separate the first barrel portion 26 from the second barrel portion 28 .
  • the fitting 29 is shown as integral to the second barrel portion 26 , and alternatively, can be reversed, that is, it is integral to the first barrel portion 29 .
  • FIGS. 3 A- 3 C depict the method and elements of a kit useful for storage, e.g., cryopreservation, of fat grafts.
  • a fat graft 113 that is, freshly aspirated fat tissue and fat cells, are drawn into a syringe 112 .
  • the process is repeated as many times as desired, collecting, e.g., from one to 25 tubes of fat (fat graft) from the patient and mixing the fat graft with cryoprotectant.
  • the fat graft is then stored 150 at a temperature below 0° C., e.g., at ⁇ 20° C., ⁇ 80° C., or in liquid nitrogen.
  • mixing may be by any method, including shaking, inverting, placing in a shaking water bath, gently vortexing in a vortex mixture, or any suitable method that does not substantially affect viability of cells in the device.
  • free lipids if any, will be on top of the fat graft.
  • the free lipids can be decanted.
  • the vessel is flipped, and the washed fat graft 113 ′ is drawn 164 from the vessel into a syringe, and is ready to be injected into a patient.
  • free lipids are removed from the fat graft after removal of the wash solution 115 ′ prior to transfer of the fat graft 113 ′′ to a syringe 164 and 166 for delivery to a patient.
  • the fat graft can be further processed prior to use, such as by treating the graft with a collagenase, filtering to remove architectural fragments, and, optionally centrifugation to separate adipocytes from other cellular elements, including the stromal vascular fraction (SVF), comprising progenitor cells, e.g., adipose-derived stem cells (ASCs), e.g., by adherence of cells of the SVF to plastic.
  • stromal vascular fraction comprising progenitor cells, e.g., adipose-derived stem cells (ASCs), e.g., by adherence of cells of the SVF to plastic.
  • progenitor cells e.g., adipose-derived stem cells (ASCs)
  • a kit for use in preserving grafts, such as fat grafts.
  • the kit comprises suitable packaging for the elements of the kit, including boxes, molded containers or inserts 170 (shown in FIG. 3 B ), suitable indicia identifying the contents and outlining the method of use of the elements of the kit, as described in the method described above, and elsewhere herein.
  • the kit comprises two or more storage vessels as described herein, e.g., as described in reference to FIG. 1 or FIG. 2 .
  • a storage container may be included in the kit, e.g., as shown and described in the context of FIG. 3 B , and include, optionally, one or more of the following: labels for the storage vessels, label(s) for the storage container, a temperature logger, or safety seals to indicate tampering with the contents of the container.
  • the kit comprises, in suitable packaging: five, 10, 15, 20, or 25 storage vessels, one or more vessel comprising a cryoprotectant, one or more vessels comprising a wash solution, and a storage container including, optionally, a temperature logger.
  • adipocytes and SVF cells were analyzed using Countess cell counter (Invitrogen) following manufacturer's protocol.

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US16/642,585 US12023664B2 (en) 2017-09-01 2018-08-31 Method and kit for preservation of adipose tissue grafts
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CN111500444B (zh) * 2020-04-22 2021-12-07 天津大学 双层套管组织模型制作模具及制作方法
KR102827440B1 (ko) * 2020-12-24 2025-07-03 주식회사 로킷헬스케어 조직 재생 패치의 제조방법
US11679198B2 (en) * 2021-04-28 2023-06-20 Chopra Gryskiewicz, LLC System and related methods for fat harvesting

Citations (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554073A (en) * 1983-03-21 1985-11-19 Rhodia Aktiengesellschaft Device for uptake, transportation, and recovery of cell material, and for transfer of the recovered material to microscope slides
CA2193945A1 (en) 1994-07-01 1996-01-18 Can B. Hu Methods for harvesting adipose tissue containing autologous microvascular endothelial cells
JPH114682A (ja) 1997-06-16 1999-01-12 Asahi Medical Co Ltd 有核細胞保存方法、有核細胞保存用組成物及び有核細胞分離方法
US6592545B1 (en) * 1994-12-23 2003-07-15 Powderject Research Limited Particle delivery
US20040185554A1 (en) * 2003-03-17 2004-09-23 Veridian Systems Portable sampling device for airborne biological particles
US20060019233A1 (en) 2004-07-23 2006-01-26 Samih Yaghmour Delivery of high cell mass in a syringe and related methods of cryopreserving cells
US20060051865A1 (en) 2004-08-31 2006-03-09 Higgins Joel C Systems and methods for isolating stromal cells from adipose tissue and uses thereof
WO2007102635A1 (en) * 2006-03-08 2007-09-13 Sewon Cellontech Co., Ltd. Adipose tissue regeneration kit and its using method
US20090239299A1 (en) 2004-03-30 2009-09-24 Genesis Biosystems, Inc. Autologous tissue harvesting and irrigation device
US20090294385A1 (en) * 2005-05-17 2009-12-03 Universal Bio Research Co., Ltd Filtration method, filter-incorporated tip, and filtration device
JP2010150219A (ja) 2008-12-26 2010-07-08 Nipro Corp 生物試料凍結保存システム及び生物試料凍結保存方法
WO2011011055A2 (en) 2009-07-20 2011-01-27 The General Hospital Corporation D/B/A Massachusetts General Hospital Methods and compositions for improving the viability of cryopreserved cells
US7989205B2 (en) 2005-10-06 2011-08-02 American Cryostem Corporation Cell culture media, kits and methods of use
US20120058027A1 (en) * 2009-05-09 2012-03-08 Moohan Enterprise Co., Ltd. Separable Test Tube for Use in a Centrifugal Separator
US20130123747A1 (en) 2010-05-20 2013-05-16 Carlo Tremolada Device and method for preparing tissue, particularly adipose tissue
WO2013070899A1 (en) 2011-11-08 2013-05-16 Auxocell Laboratories, Inc. Systems and methods for processing cells
US20140024011A1 (en) * 2012-07-23 2014-01-23 AdiCyte, Inc. Method for Preparing Adipose Tissue
CN103651331A (zh) 2013-12-13 2014-03-26 青岛中天干细胞工程有限公司 脂肪干细胞库构建方法
WO2015059798A1 (ja) * 2013-10-24 2015-04-30 学校法人東京電機大学 細胞または組織の輸送装置
US20160066563A1 (en) 2012-06-07 2016-03-10 David Moscatello Compositions and methods for collecting, washing, cryopreserving, recovering and return of lipoaspirates to physician for autologous adipose transfer procedures
US20160333305A1 (en) 2015-05-15 2016-11-17 Black Tie Medical Inc. Device and Method for Breaking Down and Sizing Harvested Fat
US20170203040A1 (en) 2015-08-20 2017-07-20 Aurastem Llc Liposuction device and use thereof
US10014079B2 (en) 2010-06-07 2018-07-03 American Cryostem Corporation Business method for collection, processing, cryogenic storage and distribution of a biologic sample material
US20180187157A1 (en) 2014-12-31 2018-07-05 Micheal MOELLER Human serum for cell culture medium for clinical growth of human adipose stromal cells
US10154664B2 (en) 2011-10-06 2018-12-18 David K Moscatello Systems and methods for the digestion of adipose tissue samples obtained from a client for cryopreservation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101951055B1 (ko) * 2010-12-16 2019-02-21 인제네론 인코포레이티드 조직 검체로부터 세포 및 세포가 풍부한 기질의 강화된 회수를 위한 방법 및 기구

Patent Citations (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554073A (en) * 1983-03-21 1985-11-19 Rhodia Aktiengesellschaft Device for uptake, transportation, and recovery of cell material, and for transfer of the recovered material to microscope slides
CA2193945A1 (en) 1994-07-01 1996-01-18 Can B. Hu Methods for harvesting adipose tissue containing autologous microvascular endothelial cells
US5744360A (en) 1994-07-01 1998-04-28 Baxter International Inc. Methods for harvesting adipose tissue containing autologous microvascular endothelial cells
US6592545B1 (en) * 1994-12-23 2003-07-15 Powderject Research Limited Particle delivery
JPH114682A (ja) 1997-06-16 1999-01-12 Asahi Medical Co Ltd 有核細胞保存方法、有核細胞保存用組成物及び有核細胞分離方法
US20040185554A1 (en) * 2003-03-17 2004-09-23 Veridian Systems Portable sampling device for airborne biological particles
US20090239299A1 (en) 2004-03-30 2009-09-24 Genesis Biosystems, Inc. Autologous tissue harvesting and irrigation device
US20060019233A1 (en) 2004-07-23 2006-01-26 Samih Yaghmour Delivery of high cell mass in a syringe and related methods of cryopreserving cells
WO2006012613A1 (en) 2004-07-23 2006-02-02 Amgen, Inc. Delivery of high cell mass in a syringe and related methods of cryopreserving cells
JP2008507563A (ja) 2004-07-23 2008-03-13 アムジエン・インコーポレーテツド シリンジでの大きな細胞塊の送出、および細胞を凍結保存する関連の方法
US20080176326A1 (en) 2004-07-23 2008-07-24 Amgen Inc. Delivery of high cell mass in a syringe and related methods of cryopreserving cells
US20060051865A1 (en) 2004-08-31 2006-03-09 Higgins Joel C Systems and methods for isolating stromal cells from adipose tissue and uses thereof
US20090294385A1 (en) * 2005-05-17 2009-12-03 Universal Bio Research Co., Ltd Filtration method, filter-incorporated tip, and filtration device
US7989205B2 (en) 2005-10-06 2011-08-02 American Cryostem Corporation Cell culture media, kits and methods of use
WO2007102635A1 (en) * 2006-03-08 2007-09-13 Sewon Cellontech Co., Ltd. Adipose tissue regeneration kit and its using method
JP2010150219A (ja) 2008-12-26 2010-07-08 Nipro Corp 生物試料凍結保存システム及び生物試料凍結保存方法
US20120058027A1 (en) * 2009-05-09 2012-03-08 Moohan Enterprise Co., Ltd. Separable Test Tube for Use in a Centrifugal Separator
WO2011011055A2 (en) 2009-07-20 2011-01-27 The General Hospital Corporation D/B/A Massachusetts General Hospital Methods and compositions for improving the viability of cryopreserved cells
US20120128641A1 (en) 2009-07-20 2012-05-24 The General Hospital Corporation D/B/A Massachusetts General Hospital Methods and compositions for improving the viability of cryopreserved cells
JP2012533620A (ja) 2009-07-20 2012-12-27 ザ ジェネラル ホスピタル コーポレーション ディー／ビー／エイ マサチューセッツ ジェネラル ホスピタル 凍結保存細胞の生存率を向上させるための方法および組成物
US20170000969A1 (en) * 2010-05-20 2017-01-05 Lipogems International S.P.A. Device and kit for preparing tissue, particularly adipose tissue, for transplantation from lobular fat extracted by liposuction
JP2013526868A (ja) 2010-05-20 2013-06-27 リポジェムズ インターナショナル ソシエタ ア レスポンサビリタ リミタータ 脂肪吸引により抽出された小葉脂肪から移植用の組織、特に脂肪組織、を準備するための装置及び方法
US20130123747A1 (en) 2010-05-20 2013-05-16 Carlo Tremolada Device and method for preparing tissue, particularly adipose tissue
US10014079B2 (en) 2010-06-07 2018-07-03 American Cryostem Corporation Business method for collection, processing, cryogenic storage and distribution of a biologic sample material
US10154664B2 (en) 2011-10-06 2018-12-18 David K Moscatello Systems and methods for the digestion of adipose tissue samples obtained from a client for cryopreservation
WO2013070899A1 (en) 2011-11-08 2013-05-16 Auxocell Laboratories, Inc. Systems and methods for processing cells
US8893995B2 (en) 2011-11-08 2014-11-25 Auxocell Laboratories, Inc. Systems and methods for processing cells
US20160066563A1 (en) 2012-06-07 2016-03-10 David Moscatello Compositions and methods for collecting, washing, cryopreserving, recovering and return of lipoaspirates to physician for autologous adipose transfer procedures
US20140024011A1 (en) * 2012-07-23 2014-01-23 AdiCyte, Inc. Method for Preparing Adipose Tissue
WO2015059798A1 (ja) * 2013-10-24 2015-04-30 学校法人東京電機大学 細胞または組織の輸送装置
CN103651331A (zh) 2013-12-13 2014-03-26 青岛中天干细胞工程有限公司 脂肪干细胞库构建方法
US20180187157A1 (en) 2014-12-31 2018-07-05 Micheal MOELLER Human serum for cell culture medium for clinical growth of human adipose stromal cells
US20160333305A1 (en) 2015-05-15 2016-11-17 Black Tie Medical Inc. Device and Method for Breaking Down and Sizing Harvested Fat
US20170203040A1 (en) 2015-08-20 2017-07-20 Aurastem Llc Liposuction device and use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
American Society of Plastic Surgeons, Plastic Surgery Statistics Report, 2016, pp. 1-25, https://www.plasticsurgery.org/news/plastic-surgery-statistics (specification).
Cui et al., "The Search for a Useful Method for the Optimal Cryopreservation of Adipose Aspirates: Part II. In Vivo Study", Aesthetic Surgery Journal, 2010, pp. 451-456, vol. 30:3.
Xiangdong et al. "The Search for a Useful Method for the Optimal Cryopreservation of Adipose Aspirates: Part II. In Vivo Study", Aesthetic Surgery Journal, 2010, pp. 451-456, vol. 30, No. 3.

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