US1834536A - Fermentation device - Google Patents

Fermentation device Download PDF

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Publication number
US1834536A
US1834536A US203378A US20337827A US1834536A US 1834536 A US1834536 A US 1834536A US 203378 A US203378 A US 203378A US 20337827 A US20337827 A US 20337827A US 1834536 A US1834536 A US 1834536A
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reservoir
tube
liquid
filling neck
flask
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US203378A
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George G Schaut
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N7/00Analysing materials by measuring the pressure or volume of a gas or vapour
    • G01N7/14Analysing materials by measuring the pressure or volume of a gas or vapour by allowing the material to emit a gas or vapour, e.g. water vapour, and measuring a pressure or volume difference
    • G01N7/18Analysing materials by measuring the pressure or volume of a gas or vapour by allowing the material to emit a gas or vapour, e.g. water vapour, and measuring a pressure or volume difference by allowing the material to react
    • G01N7/20Analysing materials by measuring the pressure or volume of a gas or vapour by allowing the material to emit a gas or vapour, e.g. water vapour, and measuring a pressure or volume difference by allowing the material to react the reaction being fermentation

Definitions

  • My invention relates to fermentation devices.
  • the object is'to provide a device for which is noteasily upset.
  • the device comprises in an integral body a reservoir portion and a tube or gas receiving portion, and the body is so formed as to provide the bottom wall of the reservoir portion in a flat or horizontal plane, with the tube or gas receiving portion having its lower end associated with said reservoir portion in close relationship with said flat bottom portion, so as to form a structure that will .rest firmly upon said bottom portion with the center of gravity well within the margin thereof so that there will be no danger of upsetting.
  • the tube or gas receiving portion is in communication with said reservoir portion at the bottom thereof, and without obstruction.
  • Fig. 1 is a side elevation when resting normally on a supporting surface.
  • Fig. 2 is a cross-section on line 2, 2 of Fig. 1.
  • Fig. 8 is an elevation showing the usual position of the device when supported between the fingers.
  • Fig. 1 the device is shown in normal operative position when resting upon a horizontal supporting surface.
  • the reservoir I portion 4 is provided with the flat or slightly convex bottom 5 and the open neck portion 6.
  • the reservoir portion is of dome-shaped formation having its axis perpendicular to the plane of the bottom.
  • the neck portion 6 is positioned to one side of said axis, at an inclination thereto.
  • the normally vertical tube or gas collector 7 is closed at the top and is provided with the right angle bend 8 which connects with the reservoir'portion 4 at or near the bottom thereof on the opposite side from that of the neck portion 6.
  • the tube 10 is preferably slightly flattened forming an oblong opening 8 be-.
  • the nutrient medium, containing the sugar or the like is introduced into the reser: voir 4.
  • Thedevice and contents are then sterilized andthe subject-matter to be tested is then introduced into the medium.
  • the reservoir is more than half filled with the medium and subject-matter to be treated. After thorough mixing of the medium and subj eat-matter the device is tilted to cause the liquid to flow into and fill the tube 7.
  • the apparatus with its contents is subjected to the usual incubator process and the gas formed or generated within the apparatus will rise to the top of the tube displacing the liquid therein.
  • Suitable gradua tions or horizontal marks 10 are provided upon the tube at proper intervals to represent the amount of the gas content.
  • a fermentation flask comprising, in an integral structure, a semi-spherical domeshaped reservoir symmetrical about a vertical axis, the bottom of which forming a substantially fiat supporting base, a communicating tube extending vertically from the bottom of the reservoir and to one side thereof to a point substantially above the horizontal plane of the top thereof, and permanently closed at itsupper end, and a filling neck extending from the upper part of the reservoir, positioned-to one side of the vertical axis thereof and on the opposite side thereof from that of the verticaltube.
  • a fermentation flask comprising, in an integral structure, a semi-spherical dome shaped reservoir symmetrical about a vertical axis, the bottom of the reservoir forming a substantially flat supporting base, a communicating tube extending vertically from the bottom of the reservoir and to one side thereof to a point substantially above the horizontal plane of the top thereof, and permanently closed at its upper end, and a filling neck extending from the upper part of the reservoir, positioned to one side of the vertical axis thereof and on the opposite side thereof from that of the vertical tube, said filling neck being so positioned and at such an angle with respect to saidvertical axis as V to permit the necessary tilting of thereservoir to cause the flow of liquid from the reservoir to fill the tube without spilling into the filling neck and also to allow the complete emptying of said vertical tube into the reservoir without spilling into the filling neck.
  • a fermentation flask comprising, in an integral structure, a semi-spherical domeshaped reservoir symmetrical about a vertical axis, the bottom of the reservoir forming a substantially flat base, a tubeextending vertically from the bottom of the reservoir and to one side thereof to a' point substantially above thehorizontal plane of the top thereof, and permanently closed at its upper end, and a filling neck extending from the upper part of the reservoir, positioned to one side of the vertical axis thereof and on the opposite side thereof from that of the vertical tube, the several parts being relatively proportioned and disposed to permit the flask to lielongitudinally upon a horizontal supporting surface while maintaining about ten per cent of gas at the closed end of the tube and also maintaining the liquid in the flask separated from the stopper in the filling neck.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Description

1931. G. G. SCHAUT FERMENTATION DEVICE Filed July 5, 1927 fulenior Geo 's 0. 802mm? Pa ented Dec. 1, 1931 U ITED STATES GEoRq G. soHAU'r, or HiLAJJELPHIA. PENNSYLVANIA r RMnNrATIoN nEvrcE.
, Application filed July 1927. Serial 203,378.
My invention relates to fermentation devices. The object is'to provide a device for which is noteasily upset.
In its essential construct-ion the device comprises in an integral body a reservoir portion and a tube or gas receiving portion, and the body is so formed as to provide the bottom wall of the reservoir portion in a flat or horizontal plane, with the tube or gas receiving portion having its lower end associated with said reservoir portion in close relationship with said flat bottom portion, so as to form a structure that will .rest firmly upon said bottom portion with the center of gravity well within the margin thereof so that there will be no danger of upsetting. The tube or gas receiving portion is in communication with said reservoir portion at the bottom thereof, and without obstruction.
Referring to the drawings, which illustrate merely by way of example a suitable embodiment of my invention :7
Fig. 1 is a side elevation when resting normally on a supporting surface.
Fig. 2 is a cross-section on line 2, 2 of Fig. 1.
Fig. 8 is an elevation showing the usual position of the device when supported between the fingers.
In Fig. 1 the device is shown in normal operative position when resting upon a horizontal supporting surface. The reservoir I portion 4 is provided with the flat or slightly convex bottom 5 and the open neck portion 6. The reservoir portion is of dome-shaped formation having its axis perpendicular to the plane of the bottom. The neck portion 6 is positioned to one side of said axis, at an inclination thereto. The normally vertical tube or gas collector 7 is closed at the top and is provided with the right angle bend 8 which connects with the reservoir'portion 4 at or near the bottom thereof on the opposite side from that of the neck portion 6. At the point of connection between the tube 7 and reservoir 4:, the tube 10 is preferably slightly flattened forming an oblong opening 8 be-. tween theitube and the reservoir, the greater axis of the said'opening extending horizontally. This arrangement is made in'order to' keep the opening 8 as near the bottom of the reservoir as possible, so that when the device is tilted as shown in Fig. 8 in the manner hereinafter described, the danger of the liquid flowing from the tube back to the reservoir is obviated. The water level in-the reservoir when so tilted is indicated by the numeral 9. The tube portion 7 is provided with the usual graduation marks 10. (55
In operation:
The nutrient medium, containing the sugar or the like is introduced into the reser: voir 4. Thedevice and contents are then sterilized andthe subject-matter to be tested is then introduced into the medium. The reservoir is more than half filled with the medium and subject-matter to be treated. After thorough mixing of the medium and subj eat-matter the device is tilted to cause the liquid to flow into and fill the tube 7.
It is sometimes necessary to return liquid from the tube 7 back to the reservoir 4:, before beginning the fermentation process. This'is secured by tilting the flask in the opposite direction from that above described.
It is important that, in these tilting operations', the liquid shall not contact with the cotton stopper which closes the filling neck 6; The desired result, in this respect, is acs5 complished bythe proper conformation and capacity content of the reservoir and by the proper disposition, as shown, of the filling neck with respect to the reservoir conformation, so that, in either tilting operation to fill or to emptythe tube, no liquid enters the filling neck 6. It is also important, in case the flask is overset after fermentation, that the liquid should not enter the filling neck and also that the gas which has accumulated in the top of the tube shall not return to the reservoir. The desired result in this respect is accomplished by the formation, as shown,
of the part 8, connecting thetube with the reservoir, relatively to said reservoir fo'rmabe higher than the closed end of the tube,
when the flask is at rest with the tube in substantially horizontal position.
It frequently happens that several of these devices, when so filled, are lifted by engaging the tubes between the several fingers of the hand. This permits the tubes to assume an inclined position as in Fig. 3, and it is important that in so tilting, the liquid remaining in the reservoir shall not assume a level. below the top of opening 8, as in which case air would flow into the tube, and thus permit the fluid to flow therefromto the reservoir; The level of the liquid in the reservoir, when so tilted, is indicated by the line 9 which is sufliciently above the top of opening 8.
The apparatus with its contents is subjected to the usual incubator process and the gas formed or generated within the apparatus will rise to the top of the tube displacing the liquid therein.- Suitable gradua tions or horizontal marks 10 are provided upon the tube at proper intervals to represent the amount of the gas content.
The particular advantage of the device is that it is integral and self-contained, needing no special supporting device. There are no separate parts to be assembled. It is inexpensive to manufacture and easy to handle and clean. lVliat I claim is 1. A fermentation flask comprising, in an integral structure, a semi-spherical domeshaped reservoir symmetrical about a vertical axis, the bottom of which forming a substantially fiat supporting base, a communicating tube extending vertically from the bottom of the reservoir and to one side thereof to a point substantially above the horizontal plane of the top thereof, and permanently closed at itsupper end, and a filling neck extending from the upper part of the reservoir, positioned-to one side of the vertical axis thereof and on the opposite side thereof from that of the verticaltube.
2. A fermentation flask comprising, in an integral structure, a semi-spherical dome shaped reservoir symmetrical about a vertical axis, the bottom of the reservoir forming a substantially flat supporting base, a communicating tube extending vertically from the bottom of the reservoir and to one side thereof to a point substantially above the horizontal plane of the top thereof, and permanently closed at its upper end, and a filling neck extending from the upper part of the reservoir, positioned to one side of the vertical axis thereof and on the opposite side thereof from that of the vertical tube, said filling neck being so positioned and at such an angle with respect to saidvertical axis as V to permit the necessary tilting of thereservoir to cause the flow of liquid from the reservoir to fill the tube without spilling into the filling neck and also to allow the complete emptying of said vertical tube into the reservoir without spilling into the filling neck. 3. A fermentation flask comprising, in an integral structure, a semi-spherical domeshaped reservoir symmetrical about a vertical axis, the bottom of the reservoir forming a substantially flat base, a tubeextending vertically from the bottom of the reservoir and to one side thereof to a' point substantially above thehorizontal plane of the top thereof, and permanently closed at its upper end, and a filling neck extending from the upper part of the reservoir, positioned to one side of the vertical axis thereof and on the opposite side thereof from that of the vertical tube, the several parts being relatively proportioned and disposed to permit the flask to lielongitudinally upon a horizontal supporting surface while maintaining about ten per cent of gas at the closed end of the tube and also maintaining the liquid in the flask separated from the stopper in the filling neck. 7
GEORGE G. SCHAUT.
US203378A 1927-07-05 1927-07-05 Fermentation device Expired - Lifetime US1834536A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3868224A (en) * 1973-11-01 1975-02-25 Esb Inc Disposable gas measuring pipet
US3884765A (en) * 1973-10-26 1975-05-20 Nasa Automatic microbial transfer device
US4293643A (en) * 1979-01-31 1981-10-06 Ohtake Works Company, Ltd. Rotary shaking culture measuring method and apparatus
US5583044A (en) * 1993-06-11 1996-12-10 Ruksenas; M. A. Fluid sampler and testing unit
US20060096367A1 (en) * 2004-11-08 2006-05-11 Meyer William V Apparatus for measuring gas exchange

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3884765A (en) * 1973-10-26 1975-05-20 Nasa Automatic microbial transfer device
US3868224A (en) * 1973-11-01 1975-02-25 Esb Inc Disposable gas measuring pipet
US4293643A (en) * 1979-01-31 1981-10-06 Ohtake Works Company, Ltd. Rotary shaking culture measuring method and apparatus
US5583044A (en) * 1993-06-11 1996-12-10 Ruksenas; M. A. Fluid sampler and testing unit
US20060096367A1 (en) * 2004-11-08 2006-05-11 Meyer William V Apparatus for measuring gas exchange
US7047832B1 (en) 2004-11-08 2006-05-23 Oporgenics, Inc., Llc Apparatus for measuring gas exchange

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