US2799660A - Blood test composition and method - Google Patents

Blood test composition and method Download PDF

Info

Publication number: US2799660A
Authority: US; United States
Prior art keywords: tablet; blood; test; water; urine
Prior art date: 1954-04-15
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

US423524A

Other languages

English (en)

Inventor

Richard S Nicholls

Dale E Fonner

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bayer Corp

Original Assignee

Miles Laboratories Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1954-04-15

Filing date

1954-04-15

Publication date

1957-07-16

1952-05-07 Priority to GB11558/52A priority Critical patent/GB708996A/en

1954-04-15 Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc

1954-04-15 Priority to US423524A priority patent/US2799660A/en

1954-12-23 Priority to GB37258/54A priority patent/GB772374A/en

1955-01-11 Priority to FR1119775D priority patent/FR1119775A/fr

1955-01-14 Priority to CH339401D priority patent/CH339401A/de

1955-03-05 Priority to DEM26333A priority patent/DE1004398B/de

1957-07-16 Application granted granted Critical

1957-07-16 Publication of US2799660A publication Critical patent/US2799660A/en

1974-07-16 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

239000000203 mixture Substances 0.000 title claims description 37
238000000034 method Methods 0.000 title description 8
238000009534 blood test Methods 0.000 title description 2
210000004369 blood Anatomy 0.000 claims description 30
239000008280 blood Substances 0.000 claims description 30
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 24
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 claims description 14
239000002253 acid Substances 0.000 claims description 10
RNVCVTLRINQCPJ-UHFFFAOYSA-N o-toluidine Chemical compound CC1=CC=CC=C1N RNVCVTLRINQCPJ-UHFFFAOYSA-N 0.000 claims description 7
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 6
HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 claims description 6
159000000021 acetate salts Chemical class 0.000 claims description 3
239000011973 solid acid Substances 0.000 claims description 3
239000002195 soluble material Substances 0.000 claims 1
239000003826 tablet Substances 0.000 description 68
238000012360 testing method Methods 0.000 description 37
210000002700 urine Anatomy 0.000 description 25
239000000463 material Substances 0.000 description 19
VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 16
239000001639 calcium acetate Substances 0.000 description 16
235000011092 calcium acetate Nutrition 0.000 description 16
229960005147 calcium acetate Drugs 0.000 description 16
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 9
239000003153 chemical reaction reagent Substances 0.000 description 9
UHCGLDSRFKGERO-UHFFFAOYSA-N strontium peroxide Chemical compound [Sr+2].[O-][O-] UHCGLDSRFKGERO-UHFFFAOYSA-N 0.000 description 9
229920002261 Corn starch Polymers 0.000 description 8
FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 8
239000008120 corn starch Substances 0.000 description 8
229940099112 cornstarch Drugs 0.000 description 8
239000000049 pigment Substances 0.000 description 8
230000035945 sensitivity Effects 0.000 description 8
239000011975 tartaric acid Substances 0.000 description 8
235000002906 tartaric acid Nutrition 0.000 description 8
238000004040 coloring Methods 0.000 description 7
238000009472 formulation Methods 0.000 description 5
150000002978 peroxides Chemical class 0.000 description 5
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
229920002472 Starch Polymers 0.000 description 4
239000013049 sediment Substances 0.000 description 4
229910052708 sodium Inorganic materials 0.000 description 4
239000007787 solid Substances 0.000 description 4
239000008107 starch Substances 0.000 description 4
235000019698 starch Nutrition 0.000 description 4
239000000454 talc Substances 0.000 description 4
229910052623 talc Inorganic materials 0.000 description 4
235000012222 talc Nutrition 0.000 description 4
LUKPNZHXJRJBAN-UHFFFAOYSA-N 4-(4-amino-3-methylphenyl)-2-methylaniline;hydron;dichloride Chemical compound [Cl-].[Cl-].C1=C([NH3+])C(C)=CC(C=2C=C(C)C([NH3+])=CC=2)=C1 LUKPNZHXJRJBAN-UHFFFAOYSA-N 0.000 description 3
BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 3
239000011734 sodium Substances 0.000 description 3
BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 2
229910052783 alkali metal Inorganic materials 0.000 description 2
-1 alkali metal acetates Chemical class 0.000 description 2
VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
230000000694 effects Effects 0.000 description 2
239000012530 fluid Substances 0.000 description 2
239000002198 insoluble material Substances 0.000 description 2
239000001053 orange pigment Substances 0.000 description 2
239000007800 oxidant agent Substances 0.000 description 2
SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
239000001054 red pigment Substances 0.000 description 2
238000010998 test method Methods 0.000 description 2
239000001052 yellow pigment Substances 0.000 description 2
AKVAGWOVWQDTAF-UHFFFAOYSA-N 1-bromo-4-(1-isocyanatoethyl)benzene Chemical compound O=C=NC(C)C1=CC=C(Br)C=C1 AKVAGWOVWQDTAF-UHFFFAOYSA-N 0.000 description 1
FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
SPAGIJMPHSUYSE-UHFFFAOYSA-N Magnesium peroxide Chemical compound [Mg+2].[O-][O-] SPAGIJMPHSUYSE-UHFFFAOYSA-N 0.000 description 1
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
229910000288 alkali metal carbonate Inorganic materials 0.000 description 1
150000008041 alkali metal carbonates Chemical class 0.000 description 1
229910052784 alkaline earth metal Inorganic materials 0.000 description 1
150000001342 alkaline earth metals Chemical class 0.000 description 1
ZJRXSAYFZMGQFP-UHFFFAOYSA-N barium peroxide Chemical compound [Ba+2].[O-][O-] ZJRXSAYFZMGQFP-UHFFFAOYSA-N 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
235000010216 calcium carbonate Nutrition 0.000 description 1
HBHZKFOUIUMKHV-UHFFFAOYSA-N chembl1982121 Chemical compound OC1=CC=C2C=CC=CC2=C1N=NC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O HBHZKFOUIUMKHV-UHFFFAOYSA-N 0.000 description 1
ZLFVRXUOSPRRKQ-UHFFFAOYSA-N chembl2138372 Chemical compound [O-][N+](=O)C1=CC(C)=CC=C1N=NC1=C(O)C=CC2=CC=CC=C12 ZLFVRXUOSPRRKQ-UHFFFAOYSA-N 0.000 description 1
239000003086 colorant Substances 0.000 description 1
WZHCOOQXZCIUNC-UHFFFAOYSA-N cyclandelate Chemical class C1C(C)(C)CC(C)CC1OC(=O)C(O)C1=CC=CC=C1 WZHCOOQXZCIUNC-UHFFFAOYSA-N 0.000 description 1
239000003085 diluting agent Substances 0.000 description 1
239000007938 effervescent tablet Substances 0.000 description 1
210000003743 erythrocyte Anatomy 0.000 description 1
239000004615 ingredient Substances 0.000 description 1
230000001788 irregular Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 1
229960004995 magnesium peroxide Drugs 0.000 description 1
150000007524 organic acids Chemical class 0.000 description 1
239000000546 pharmaceutical excipient Substances 0.000 description 1
229910052700 potassium Inorganic materials 0.000 description 1
235000011056 potassium acetate Nutrition 0.000 description 1
BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
235000011181 potassium carbonates Nutrition 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
239000004576 sand Substances 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
235000011182 sodium carbonates Nutrition 0.000 description 1
229910052712 strontium Inorganic materials 0.000 description 1
CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000007916 tablet composition Substances 0.000 description 1
210000001550 testis Anatomy 0.000 description 1
WODGXFMUOLGZSY-UHFFFAOYSA-J tetrasodium phosphonatooxy phosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OOP([O-])([O-])=O WODGXFMUOLGZSY-UHFFFAOYSA-J 0.000 description 1
MSLRPWGRFCKNIZ-UHFFFAOYSA-J tetrasodium;hydrogen peroxide;dicarbonate Chemical compound [Na+].[Na+].[Na+].[Na+].OO.OO.OO.[O-]C([O-])=O.[O-]C([O-])=O MSLRPWGRFCKNIZ-UHFFFAOYSA-J 0.000 description 1
150000004992 toluidines Chemical class 0.000 description 1
AQLJVWUFPCUVLO-UHFFFAOYSA-N urea hydrogen peroxide Chemical compound OO.NC(N)=O AQLJVWUFPCUVLO-UHFFFAOYSA-N 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/725—Haemoglobin using peroxidative activity
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/904—Oxidation - reduction indicators

Definitions

This invention which relates to a diagnostic composition for detecting occult blood in animal fluids is a conatnt
the present invention is directed to a tablet which is 7,
the tablet of our invention is formed of a' mixture of a blood indicator such as, for example, o-a blood indicator
tolidine, o-toluidine, benzidine, o-tolidine-dihydrochloride and similar compounds an oxidizing agent of the peroxide type such as sodium carbonate peroxide, sodium perbor silicate, strontium peroxide, sodium pyrophosphate peroxide, barium peroxide, magnesium peroxide, and urea peroxide; an acetate such as calcium acetate, sodium acetate, potassium acetate, lithium acetate and the like; a water insoluble pigment or dye; and a water-soluble solid acid which is stronger than acetic acid so that it can react with the aforementioned acetate salt to produce acetic acid.
an oxidizing agent of the peroxide type such as sodium carbonate peroxide, sodium perbor silicate, strontium peroxide, sodium pyrophosphate peroxide, barium peroxide, magnesium peroxide, and urea peroxide
an acetate such as calcium acetate, sodium acetate, potassium acetate
Such acids are citric, fumaric, itaconic, maleic, rnalic, malonic, and mandelic acids.
an effervescent couple comprising, for example, an acid in solid dry form and a carbonate or bicarbonate which is preferably any alkaline earth or alkali metal carbonate or bicarbonate such as sodium, potassium, and calcium carbonates and bicarbonates. Diluents and excipients' of the kind conventionally used in preparation of tablets are likewise used in conjunction with the aforementioned materials.
the above composition is prepared in tablet form, each tablet containing conveniently 2 grains of material and being conveniently A; inch thick, and about A inch in diameter.
the composition contain, in addition to the blood indicator and an oxidizing agent, an acetate, a water soluble organic acid such as tartaric acid which is capable of releasing acetic acid when contacted with an acetate, and an effervescent couple. Without the co-presence of these materials neither the degree of sensitivity nor the degree of reproducibility of test results will be adequate.
Strontium peroxide From '5 to 15, preferably 9. Tartaric acid From 20 to 60, preferably 30. Calcium acetate From 20 to 75, preferably 60.
Corn starch From 1 to 10, preferably 5.
Effervescent couple From 5 to 20, preferably 10.
o-Tolidine From 0.5-4, preferably 1.
Stront um peroxide From 5-15 preferably 7.5.
Succ mc acid From 20-75, preferably 40.
Calcium acetate From 20-75, preferably 60.
Corn starch From 1-10, preferably 5.
Effervescent couple From 5-20, preferably 10.
o-Tolidine From 1-6, preferably 4.
Sodium perbor silicate From 8-20, preferably 10.
Succrnic acid From 20-75, preferably 40.
Calcium acetate From 20-75, preferably 60.
Corn starch From 1-10, preferably 5.
Effervescent couple From 5-20, preferably 10.
o-Tolidine From 0.5-4, preferably 1.
Strontium peroxide Itaconic acld Calcium acetate Corn starch Effervescent coup From 5-15, preferably 7.5. From 20-75, preferably 40. From 20-75, preferably 60. From 1-10, preferably 5.
Benzidine hydrochloride From Strontium peroxide 'lartaric acid 1-5, preferably 1. 5-15, preferably 7.5. 20-60, preferably 30.
Calcium acetate From 20-75, preferably 60.
Efiervescent couple From 5-20, preferably 10.
the present invention is particularly useful in the determination of occult blood in the presence of urine and, in sharp contrast with diagnostic compositions of the prior art, is sensitive to the extent of detecting of one part blod in 100,000 of uncentrifuged urine.
tablet A is of the general type of prior art compositions such as are described, for example, in U. S. Patent 2,290,436, that tablet B is the composition of tablet A to which calcium acetate has been added and that tablet 'C is of the same composition as tablet A to which both calcium acetate and efliervescent couple have been added.
Tablet D is the same composition as tablet C so modified as to include a water-insoluble pigment or dye.
a water-insoluble pigment or dye To incorporate the pigment or dye in the tablet it is simply necessary that it is dry and finely divided and it is then mixed intimately with the remainder of the composition prior to compressing the material into tablets.
the amount of pigment required would obviously vary depending on its hiding power and opacity, therefore the amount considered most satisfactory for any given pigment must be determined by trial. However, the necessary amount is generally not very much, being present
the superior performance of tablet B over tablet A shows that the addition of calcium acetate increases considerably'the sensitivity of the test. Further it is obvious that tablet C is more uniform in reaction time than tablet B and in addition the sensitivity of the tablet within a given time period is very substantially improved.
the tablets B and C were both compared by testing them with the sediments obtained by centrifuging samples of urine containing very small quantities of blood.
blood was diluted with normal urine to produce blood concentrations of 1 part in 50,000; 1 part in 100,- 000; 1 part in 300,000 and 1 part in 1,000,000 parts of urine.
15 cc. of one of the specimens was centrifuged in a conical tube at moderate speed. The *the mannerdescribed can be illustrated by the following supernatant liquid was drawn off and the sediment which remained tested by placing 1 drop of it on filter paper and then proceeding with the test as previously described.
a Perthe added coloring material should be a water-insoluble Ohlde Whlhh h hberate hydrogeh. pehoxlde Water pigment or dye so that it will not leach away from the soluble .sohd.acld strohghr than achhc i ah acetate reagent material with which it is combined in the tablet Shh h whl rhact Wlth the last sand and to Produce when the whole mass is wetted as described in the test.
the coloring materials should have the strontium peroxide, 20 to 60 parts tartaric acid, 20 to property of good opacity and hiding power so that they parts calcium acetate, 1 to 10 parts corn starch, 5 to 20 shall stand out well in contrast to blue and further they parts of a solid effervescent couple, and a minor amount shall cover up any tendency of the material of the test of a water-insoluble material contrasting in color with itself to turn blue. 75 the color formed in combination with blood by said The advantage of coloring the tableted composition in o-tolidine.
composition-in tablet form comprisinglarmemberaofthe-group consisting-of o-tolidine, o--
o-tolidine consisting of o-tolidine, o-toluidine, benzidine and o-tolidine dihydrochloride, a peroxide which will liberatelhy: drogen peroxide, an acetate selected from the group;-
a method of determining occult blood in urine which comprises rwettingma portion of dry filter paper withaurine, placing-ontthe ..urine wetted portion of said paper, a tablet, composed of a mixture comprising by a water soluble acid stronger than acetic acid, and Ian 15 gh fIOm-abOI-It Parts of wmpound effervescent couple.
a diagnostic composition in tablet form f0'l' 'dfi' tecting blood in the presence of urine comprising by weight from 0.5 to 4 parts o-tolidine, 5 to parts of 75 parts of calcium acetate, 1 to 10 parts cornstarch, and 5 to parts of a solid eflervescent couple, said tablet effecting a color change on urine wetted filter paper when placed on'suchlpaper andthe 'tablet'conta-cted with a small amount of water.
A'diagnosti'c 'composition'in tablet form for detecting blood intliepresence of urine comprising 1 part of 'o-tolidine; 9parts of strontium peroxide, parts of tartaric acid, parts of calcium acetate, 5 parts of 'cornstarch, and 10'parts' of'a solid efiervescent couple, the 30 tablet efie'ctingea color change on urine-Wetted filter paper upon which said "tablet is placed, after a small amount of water is placed on said 'table't.
composition of claim 2 wherein the said water- 5 insoluble material is'selected from the group consisting of red pigments, yellow pigments, and orange pigments.

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Hematology (AREA)
Pathology (AREA)
Biomedical Technology (AREA)
General Physics & Mathematics (AREA)
Urology & Nephrology (AREA)
Biochemistry (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Cell Biology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Investigating Or Analysing Biological Materials (AREA)

US423524A 1954-04-15 1954-04-15 Blood test composition and method Expired - Lifetime US2799660A (en)

Priority Applications (6)

Application Number	Priority Date	Filing Date	Title
GB11558/52A GB708996A (en)	1954-04-15	1952-05-07	Improvements in or relating to diagnostic compositions
US423524A US2799660A (en)	1954-04-15	1954-04-15	Blood test composition and method
GB37258/54A GB772374A (en)	1954-04-15	1954-12-23	Blood test
FR1119775D FR1119775A (fr)	1954-04-15	1955-01-11	Composition de diagnostic
CH339401D CH339401A (de)	1954-04-15	1955-01-14	Reagens zum Nachweis von Blut für Diagnosezwecke
DEM26333A DE1004398B (de)	1954-04-15	1955-03-05	Diagnostisches Praeparat in Tablettenform zum Nachweis von Blut

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US423524A US2799660A (en)	1954-04-15	1954-04-15	Blood test composition and method

Publications (1)

Publication Number	Publication Date
US2799660A true US2799660A (en)	1957-07-16

Family

ID=23679205

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
US423524A Expired - Lifetime US2799660A (en)	1954-04-15	1954-04-15	Blood test composition and method

Country Status (5)

Country	Link
US (1)	US2799660A (de)
CH (1)	CH339401A (de)
DE (1)	DE1004398B (de)
FR (1)	FR1119775A (de)
GB (2)	GB708996A (de)

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US2905594A (en) *	1956-04-25	1959-09-22	Herman J Morris	Means for detecting enzyme activity
US2970945A (en) *	1958-10-03	1961-02-07	Miles Lab	Diagnostic composition
US3068855A (en) *	1957-04-29	1962-12-18	Jr Norman B Furlong	Disposable blood-gas analyzer
US3252762A (en) *	1961-05-04	1966-05-24	Miles Lab	Stabilized occult blood diagnostic
US3335793A (en) *	1964-11-30	1967-08-15	Cities Service Oil Co	Method and composition for improving and maintaining the capacity of water injection wells
US3411887A (en) *	1964-06-15	1968-11-19	Miles Lab	Diagnostic composition
USRE28575E (en) *	1971-03-01	1975-10-21		Indicator for detecting hydrogen peroxide and peroxidative compounds containing alpha naphthoflavone
US4148611A (en) *	1978-06-28	1979-04-10	Miles Laboratories, Inc.	Test composition, device and method for the detection of peroxidatively active substances
US4175923A (en) *	1978-06-26	1979-11-27	Friend William G	Method and apparatus for occult blood testing in the home
US4394452A (en) *	1977-05-31	1983-07-19	Rohm Gmbh	Synthetic stool
EP0177244A3 (en) *	1984-09-26	1986-12-17	Warner-Lambert Company	A method for the detection of peroxidase activity
US4676950A (en) *	1984-02-03	1987-06-30	Foster Research Corporation	Indicator and test device for detecting occult blood
EP0227602A3 (en) *	1985-12-20	1988-04-06	Warner-Lambert Company	Free flowing granular indicator material for peroxidase-like activity
US4956300A (en) *	1982-01-05	1990-09-11	Helena Laboratories Corporation	Aid for determining the presence of occult blood, method of making the aid, and method of using the aid
US5081040A (en) *	1987-06-29	1992-01-14	Helena Laboratories Corporation	Composition and kit for testing for occult blood in human and animal excretions, fluids, or tissue matrixes
US5196167A (en) *	1989-04-04	1993-03-23	Helena Laboratories Corporation	Fecal occult blood test product with positive and negative controls
US5217874A (en) *	1989-04-04	1993-06-08	Helena Laboratories Corporation	Fecal occult blood test product with positive and negative controls
US5273888A (en) *	1984-01-16	1993-12-28	Helena Laboratories Corporation	Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals
US5702913A (en) *	1983-12-21	1997-12-30	Helena Laboratories Corporation	Chromgen-reagent test system
US6046019A (en) *	1991-07-09	2000-04-04	Goumeniouk; Alexander P.	Diagnostic kits and methods for making granulocyte cell counts
US6225123B1 (en) *	1997-04-30	2001-05-01	Becton Dickinson And Company	Additive preparation and method of use thereof
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US2981606A (en) *	1955-06-20	1961-04-25	Lilly Co Eli	Glucose indicator and method
US2848308A (en) *	1955-12-05	1958-08-19	Miles Lab	Composition of matter
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IT569423A (de) *	1956-04-12
JP3003152B2 (ja) *	1990-03-20	2000-01-24	吉富製薬株式会社	遊離ヘモグロビンの測定法及びそれに用いる遊離ヘモグロビン測定用試薬キット

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1952
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1954
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- 1954-12-23 GB GB37258/54A patent/GB772374A/en not_active Expired
1955
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- 1955-01-14 CH CH339401D patent/CH339401A/de unknown
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US2905594A (en) *	1956-04-25	1959-09-22	Herman J Morris	Means for detecting enzyme activity
US3068855A (en) *	1957-04-29	1962-12-18	Jr Norman B Furlong	Disposable blood-gas analyzer
US2970945A (en) *	1958-10-03	1961-02-07	Miles Lab	Diagnostic composition
DE1265453B (de) *	1961-05-04	1968-04-04	Miles Lab	Zum Nachweis von Blut bestimmtes diagnostisches Mittel
US3252762A (en) *	1961-05-04	1966-05-24	Miles Lab	Stabilized occult blood diagnostic
US3411887A (en) *	1964-06-15	1968-11-19	Miles Lab	Diagnostic composition
US3335793A (en) *	1964-11-30	1967-08-15	Cities Service Oil Co	Method and composition for improving and maintaining the capacity of water injection wells
USRE28575E (en) *	1971-03-01	1975-10-21		Indicator for detecting hydrogen peroxide and peroxidative compounds containing alpha naphthoflavone
US4394452A (en) *	1977-05-31	1983-07-19	Rohm Gmbh	Synthetic stool
US4175923A (en) *	1978-06-26	1979-11-27	Friend William G	Method and apparatus for occult blood testing in the home
US4148611A (en) *	1978-06-28	1979-04-10	Miles Laboratories, Inc.	Test composition, device and method for the detection of peroxidatively active substances
US4956300A (en) *	1982-01-05	1990-09-11	Helena Laboratories Corporation	Aid for determining the presence of occult blood, method of making the aid, and method of using the aid
US5702913A (en) *	1983-12-21	1997-12-30	Helena Laboratories Corporation	Chromgen-reagent test system
US5273888A (en) *	1984-01-16	1993-12-28	Helena Laboratories Corporation	Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals
US4676950A (en) *	1984-02-03	1987-06-30	Foster Research Corporation	Indicator and test device for detecting occult blood
EP0177244A3 (en) *	1984-09-26	1986-12-17	Warner-Lambert Company	A method for the detection of peroxidase activity
EP0227602A3 (en) *	1985-12-20	1988-04-06	Warner-Lambert Company	Free flowing granular indicator material for peroxidase-like activity
US5081040A (en) *	1987-06-29	1992-01-14	Helena Laboratories Corporation	Composition and kit for testing for occult blood in human and animal excretions, fluids, or tissue matrixes
US5196167A (en) *	1989-04-04	1993-03-23	Helena Laboratories Corporation	Fecal occult blood test product with positive and negative controls
US5217874A (en) *	1989-04-04	1993-06-08	Helena Laboratories Corporation	Fecal occult blood test product with positive and negative controls
US6046019A (en) *	1991-07-09	2000-04-04	Goumeniouk; Alexander P.	Diagnostic kits and methods for making granulocyte cell counts
US6225123B1 (en) *	1997-04-30	2001-05-01	Becton Dickinson And Company	Additive preparation and method of use thereof
US6534016B1 (en)	1997-04-30	2003-03-18	Richmond Cohen	Additive preparation and method of use thereof
EP0875756B1 (de) *	1997-04-30	2004-02-18	Becton, Dickinson and Company	Herstellung eines Additivs und Verfahren zur Verwendung
KR100473726B1 (ko) *	1997-04-30	2005-07-18	벡톤 디킨슨 앤드 컴퍼니	첨가조제물및이의사용방법

Also Published As

Publication number	Publication date
GB708996A (en)	1954-05-12
DE1004398B (de)	1957-03-14
FR1119775A (fr)	1956-06-25
GB772374A (en)	1957-04-10
CH339401A (de)	1959-06-30

Publication	Publication Date	Title
US2799660A (en)	1957-07-16	Blood test composition and method
US3814668A (en)	1974-06-04	Method and device for the semi-quantitative determination of glucose in aqueous fluids
US4673654A (en)	1987-06-16	Composition for determining peroxidase-like activity of hemoglobin
US2854317A (en)	1958-09-30	Method and composition for testing bilirubin in urine
CA1338588C (en)	1996-09-10	Composition and method of assaying liquids for specific gravity
CN106198527A (zh)	2016-12-07	一种抗坏血酸干扰多项尿液分析试纸
US5350694A (en)	1994-09-27	Composition method and device for measuring the divalent cation concentration or specific gravity of a test sample
US3654180A (en)	1972-04-04	Indicator for detecting hydrogen peroxide and peroxidative compounds containing alpha naphthoflavone
US3748096A (en)	1973-07-24	Indicator for the detection of copper ions
CA1260371A (en)	1989-09-26	Free flowing granular indicator material for peroxidase-like activity
US3016292A (en)	1962-01-09	Diagnostic composition
US3042496A (en)	1962-07-03	Diagnostic composition
US4956300A (en)	1990-09-11	Aid for determining the presence of occult blood, method of making the aid, and method of using the aid
EP0491936B1 (de)	1995-11-08	Sehr rasche feststellung von pilz-infektionen
CA1213197A (en)	1986-10-28	Home diagnostic aid and method for determining the presence of occult blood for use by the general public
DE3851304T2 (de)	1995-01-26	Verfahren und Testzusammensetzung zur Bestimmung von Enzym-Aktivität.
JPS6338665B2 (de)	1988-08-01
EP4698890A2 (de)	2026-02-25	Verfahren und kits zur schnellen, kolorimetrischen berylliumdetektion
EP0646795A1 (de)	1995-04-05	Reagens, Zusammensetzung und Verfahren zur Bestimmung von Lithium
CA2081870A1 (en)	1993-05-16	Detection of analytes in saliva using peroxide-peroxidase test systems
US2982700A (en)	1961-05-02	Diagnostic composition
JP2522970B2 (ja)	1996-08-07	体液中のクレアチニン定量用試験片及びその製法
CA2920471C (en)	2021-03-16	A chlorine analytical test element and a stabilized n,n-diethyl-p-phenylenediamine solution
CA1254115A (en)	1989-05-16	Nonenzymatic glucose test
JPH01147366A (ja)	1989-06-09	潜血検出用組成物およびそれを用いた検査体