US2927885A - Process for the depilation and bating of pelts and hides - Google Patents

Process for the depilation and bating of pelts and hides Download PDF

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Publication number
US2927885A
US2927885A US505852A US50585255A US2927885A US 2927885 A US2927885 A US 2927885A US 505852 A US505852 A US 505852A US 50585255 A US50585255 A US 50585255A US 2927885 A US2927885 A US 2927885A
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US
United States
Prior art keywords
depilation
strains
pelts
streptomyces
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US505852A
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English (en)
Inventor
Deltour Guy Henri
Ghuys Jean-Marie Julien Hubert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Labaz Sanofi NV
Original Assignee
Labaz Laboratoires SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Labaz Laboratoires SA filed Critical Labaz Laboratoires SA
Application granted granted Critical
Publication of US2927885A publication Critical patent/US2927885A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C14—SKINS; HIDES; PELTS; LEATHER
    • C14C—CHEMICAL TREATMENT OF HIDES, SKINS OR LEATHER, e.g. TANNING, IMPREGNATING, FINISHING; APPARATUS THEREFOR; COMPOSITIONS FOR TANNING
    • C14C1/00—Chemical treatment prior to tanning
    • C14C1/06—Facilitating unhairing, e.g. by painting, by liming
    • C14C1/065—Enzymatic unhairing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/886—Streptomyces
    • Y10S435/887—Streptomyces albus

Definitions

  • the proteolytic enzymes in question require, before bating proper, depilation of the pelts which is generally done by treatment in baths containing lime and alkali sulphide.
  • the depilation itself is a rather long operation often accompanied by putrifaction and attack on the collagen by; the microbic flora normally found "on the skins, which in either case results 'in a loss of leather.
  • the present invention relates to the use, both for the depilation and bathing of pelts, of a thermolabile complex, probably of enzymatic nature, secreted by certain species of Streptomyces. It is known that the Streptomyces can secrete substances in their culture medium, whose lytic activity is 'manifested in vitro by clarification of suspensions of living gram-positive bacteria such as -Micr0coccus pyogenes aureus, giving rise to.
  • Caseinolyt. Keratinolytnu Depilatory 37 C. is added thereto, the casein solution being prepared by dissolving in alkaline medium followed by neutralization. After five minutes of incubation at 37 C. 0.5m. of the mixture is removed and added to 4.5 ml. of a 1% solution of tn'choracetic acid. After 30 minutes rest at room temperature the cloudiness which is proportional to the amount of non-hydrolyzed casein 'isevaluated to obtain direct measurement of the caseinolytic activity.
  • the keratin used for this test comes from chicken feathers and is prepared by the method of H. P. Lundgreen, A. M. Stein, V. M. Koorn and R. A. OConnel, described in J. of Phys. and C011. Chem, 1948, vol. 52,
  • the caseinolytic and keratinolytic activities of the strains can be classified as follows:
  • Activity zero disappearance of less than 5% of cloudcurred in the culture filtrates of a certain number of I strains.
  • the depilatory activity of the strains can be classified in the following manner:
  • the filtrates of this treatment when they have reached the desired rate of activity and possibly concentrtated by vacuum distillation are used to effect depilation and bating in one operation.
  • This treatment operatedat a neutral or slightly alkaline pH takes only a few hours, which means not only a very considerable saving in time but also reduces the danger of collagen losses by putrefaction and, in addition, makes it possible to recover in an industrially usable condition, the hair obtained by this new method of depilation.
  • the depilatory and bating activity of the filtrates in hand, to the process used, the amount of active product used, the temperature of the baths etc.,- the speed of depilation can vary somewhat.
  • the part of the skin selected and according, on the other those which are most suitable for a given treatment of depilation and/ or bating of pelts.
  • a process for the treatment of skins and hides for the manufacture of leather by tanning which comprises contacting said skins and hides in a neutral to slightly alkaline reaction medium with cultures of Streptomyces of the Streptomyces albus species.
  • a process for the treatment of skins and hides for the manufacture of leather by tanning which comprises contacting said skins and hides in a neutral to slightly alkaline reaction medium with cultures of Streptomyces strains of the Streptomyces albus G species.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Coloring (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
US505852A 1954-05-11 1955-05-03 Process for the depilation and bating of pelts and hides Expired - Lifetime US2927885A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BE2927885X 1954-05-11

Publications (1)

Publication Number Publication Date
US2927885A true US2927885A (en) 1960-03-08

Family

ID=3896170

Family Applications (1)

Application Number Title Priority Date Filing Date
US505852A Expired - Lifetime US2927885A (en) 1954-05-11 1955-05-03 Process for the depilation and bating of pelts and hides

Country Status (5)

Country Link
US (1) US2927885A (fr)
DE (1) DE1061477B (fr)
FR (1) FR1128945A (fr)
GB (1) GB790237A (fr)
NL (1) NL93125C (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10277858B2 (en) * 2015-10-29 2019-04-30 Microsoft Technology Licensing, Llc Tracking object of interest in an omnidirectional video

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1772258A (en) * 1925-04-18 1930-08-05 Rohm & Haas Process for unhairing and preparing hides for tanning
US2041731A (en) * 1934-07-07 1936-05-26 Wallerstein Co Inc Production of leather
US2157969A (en) * 1936-02-08 1939-05-09 Rohm Otto Method for producing hides with the aid of mold tryptases
US2229420A (en) * 1937-06-29 1941-01-21 Kalle & Co Ag Unhairing process
US2278233A (en) * 1939-06-16 1942-03-31 American Cyanamid Co Mold culture
US2279908A (en) * 1939-06-16 1942-04-14 American Cyanamid Co Protein digestion

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1772258A (en) * 1925-04-18 1930-08-05 Rohm & Haas Process for unhairing and preparing hides for tanning
US2041731A (en) * 1934-07-07 1936-05-26 Wallerstein Co Inc Production of leather
US2157969A (en) * 1936-02-08 1939-05-09 Rohm Otto Method for producing hides with the aid of mold tryptases
US2229420A (en) * 1937-06-29 1941-01-21 Kalle & Co Ag Unhairing process
US2278233A (en) * 1939-06-16 1942-03-31 American Cyanamid Co Mold culture
US2279908A (en) * 1939-06-16 1942-04-14 American Cyanamid Co Protein digestion

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10277858B2 (en) * 2015-10-29 2019-04-30 Microsoft Technology Licensing, Llc Tracking object of interest in an omnidirectional video

Also Published As

Publication number Publication date
FR1128945A (fr) 1957-01-14
DE1061477B (de) 1959-07-16
GB790237A (en) 1958-02-05
NL93125C (nl) 1960-01-15

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