US3150001A - Device for use in paper chromatography - Google Patents

Device for use in paper chromatography Download PDF

Info

Publication number
US3150001A
US3150001A US213814A US21381462A US3150001A US 3150001 A US3150001 A US 3150001A US 213814 A US213814 A US 213814A US 21381462 A US21381462 A US 21381462A US 3150001 A US3150001 A US 3150001A
Authority
US
United States
Prior art keywords
paper
paper strip
tube
outer end
swing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US213814A
Other languages
English (en)
Inventor
Hrdina Jiri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Czech Academy of Sciences CAS
Original Assignee
Czech Academy of Sciences CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Czech Academy of Sciences CAS filed Critical Czech Academy of Sciences CAS
Application granted granted Critical
Publication of US3150001A publication Critical patent/US3150001A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N30/91—Application of the sample
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography

Definitions

  • FIG. 2 3mm JIRI HR DINA United States Patent 3,150,001 DEVICE FOR USE IN PAPER CHROMATOGRQPHY .lii'i Hrdiua, Prague, Czechoslovakia, assignor to Ceshoslovenslra Ahademie Vd, Prague, Czechoslovakia, an institution of Czechoslovakia Filed July 31, 1962, Ser. No. 213,814 Claims priority, applicafion Czechoslovakia, Aug. 8, 1961, 4,862/61 2 Claims. (Cl.
  • My invention relates to a device for use in methods known as paper chromatography wherein mixtures of solutes are separated by absorption in strips of filter paper for the purpose of isolation or identification of the components.
  • paper chromatography is used in the analytical control of continuing processes for example in the separation in chromatographic columns, an aliquot branched from the main current of the eluate is fed upon chromatographic paper to form a narrow trace thereupon.
  • the lmown devices of said type have many disadvantages; particularly they do not work efiiciently and reliably and are slow in operation.
  • FIG. 1 shows a sectional view of the upper head member of the feeding device indicated taken along line II--II of FIG. 1.
  • the feeding device applying a trace of the eluate upon the chromatographic paper 1, which moves in the direction of the arrow shown in FIG. 2, comprises an upper head member 2 and a lower head member 3.
  • the head member 2 supports and adjustably holds a capillary supply tube 4 through which a branched off aliquot of eluate is fed upon the paper 1.
  • the discharging end of the capillary tube contacts the paper and i preferably made of a flexible material for example Teflon which prevents the formation of drops or bubbles.
  • Said discharging end of the tube 4 is preferably inclined relative to the paper 1 as shown in FIG. 2 and contacts the same elastically with little pressure.
  • the head member 2 holds said tube by means of an elastic sleeve 20 compressible by a hollow screw 21.
  • the head member 2 is mounted on a metallic or similar tube 6 through which drying air is introduced into a nozzle 5 attached to said head member opposite the moving paper strip 1.
  • the intake end of the tube 6 is fixed in a base member 7 which is hinged by a joint 8 to the top 9 of an air chamber 10 arranged in or upon the frame of the device. From said chamber 1t) drying air enters into the tube 6 through passage 11 and tube opening 13 which is sealed by a flexible packing 12 attached to said top 9.
  • a set screw 14 mounted in the base member 7 and abutting against the top 9 serves to adjust the position of the tube 6 and thus of the head member 2 relative to the paper strip 1.
  • the lower head member 3 is mounted on the end of a metallic or similar tube 16 which with its other end is 3,150,001 Patented Sept. 22, 1964 hinged by the joint 17 to the air chamber 10 to permit a positional adjustment in vertical direction of the head member 3 relative to the paper strip 1.
  • the tube 16 enters the air chamber 10 through the opening 18 which is sealed by a flexible membrane 19, made for example from rubber. Drying air flows from chamber 10 through tube 16 into a nozzle 15 provided on the head member 3 opposite the paper strip 1 and impinges upon the underside of the paper strip 1 opposite the upper head member 2.
  • the tube 16 may be provided with adjusting means similar to those for tube 6 though its fine adjustment is not so important as that of tube 6.
  • the head member 3 may be held in position relative to the paper by a spring 53.
  • the upper head member 2 is held in operative position by its own weight and may be swung about the joint it away from the paper strip 1 to permit free access to said strip and to its supporting drum (not shown).
  • the air chamber it rests upon a base including a second chamber 22.
  • the chambers 10 and 12 are connected by conduit 26.
  • the chamber 26 is provided with an inlet opening 25 and houses a fan 23 or other air moving device driven by motor 24.
  • the air flows through conduit 26 into air chamber 1! ⁇ and on its way may be heated or cooled by a heat exchanger 27.
  • the capillary tube 4 may be attached to an ultramicropump or it may be branched otf from the main current of the eluate by means of a rotating dosimeter having its own driving means as indicated by the dotted lines in FIG. 1. Such dosimeter may be mounted upon the hinged top 9 or may replace the same if properly shaped.
  • drying air may be supplied to the head members 2 and .3 directly from an independent source by means of hoses substituting the tubes 6 and 16 provided the weight of such hoses does not impair the proper positioning of said head members relative to the moving paper strip.
  • a device for depositing traces of an eluate from a chromatographic column upon a continuously moving strip of filter paper comprising in combination (a) a support;
  • a capillary tube adapted to receive and conduct an aliquot of the eluate and adjustably held in the outer end of the first swing arm with its discharge end contacting the paper strip to deposit a trace of the aliquot of the eluate thereupon in longitudinal direction thereof and in preparation for succeeding dimensional development;
  • conduit means feeding drying air into said first and said second air nozzle, respectively.
  • a device as defined in claim 1 comprising an air chamber in the support; heating means for the air in said chamber; and at least one of said swing arms being tubular to feed air to its nozzle from said chamber.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)
US213814A 1961-08-08 1962-07-31 Device for use in paper chromatography Expired - Lifetime US3150001A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CS486261 1961-08-08

Publications (1)

Publication Number Publication Date
US3150001A true US3150001A (en) 1964-09-22

Family

ID=5392300

Family Applications (1)

Application Number Title Priority Date Filing Date
US213814A Expired - Lifetime US3150001A (en) 1961-08-08 1962-07-31 Device for use in paper chromatography

Country Status (5)

Country Link
US (1) US3150001A (de)
CH (1) CH405766A (de)
DE (1) DE1498628A1 (de)
GB (1) GB998963A (de)
SE (1) SE220284C1 (de)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3635345A (en) * 1969-03-13 1972-01-18 Jerome A Rodder Chromatography
US3766884A (en) * 1971-06-28 1973-10-23 Long Island Jewish Medical Ct Apparatus for sample application in thin layer and paper chromatography
US3857784A (en) * 1972-10-27 1974-12-31 Sindco Apparatus for applying and drying chromatographic samples
US4004548A (en) * 1975-07-02 1977-01-25 Eastman Kodak Company Chromatographic spotter
US4272482A (en) * 1979-07-02 1981-06-09 Eastman Kodak Company Metering apparatus

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1208552A (en) * 1916-05-03 1916-12-12 Frank B Dole Edge-setting machine.
US2177323A (en) * 1937-10-07 1939-10-24 Owens Corning Fiberglass Corp Method and apparatus for controlling the impregnation of textiles
US2333629A (en) * 1941-07-14 1943-11-09 Cons Water Power And Paper Com Coating process
US2644717A (en) * 1949-11-11 1953-07-07 Kopperschmidt Wilhelm Spray device for liquids, thermoplastics, molten metal, or the like
US2659343A (en) * 1950-12-01 1953-11-17 Gen Aniline & Film Corp Coating liquid applicator
US3027821A (en) * 1958-06-18 1962-04-03 Hycon Mfg Company Film processing applicator

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1208552A (en) * 1916-05-03 1916-12-12 Frank B Dole Edge-setting machine.
US2177323A (en) * 1937-10-07 1939-10-24 Owens Corning Fiberglass Corp Method and apparatus for controlling the impregnation of textiles
US2333629A (en) * 1941-07-14 1943-11-09 Cons Water Power And Paper Com Coating process
US2644717A (en) * 1949-11-11 1953-07-07 Kopperschmidt Wilhelm Spray device for liquids, thermoplastics, molten metal, or the like
US2659343A (en) * 1950-12-01 1953-11-17 Gen Aniline & Film Corp Coating liquid applicator
US3027821A (en) * 1958-06-18 1962-04-03 Hycon Mfg Company Film processing applicator

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3635345A (en) * 1969-03-13 1972-01-18 Jerome A Rodder Chromatography
US3766884A (en) * 1971-06-28 1973-10-23 Long Island Jewish Medical Ct Apparatus for sample application in thin layer and paper chromatography
US3857784A (en) * 1972-10-27 1974-12-31 Sindco Apparatus for applying and drying chromatographic samples
US4004548A (en) * 1975-07-02 1977-01-25 Eastman Kodak Company Chromatographic spotter
US4272482A (en) * 1979-07-02 1981-06-09 Eastman Kodak Company Metering apparatus

Also Published As

Publication number Publication date
SE220284C1 (de) 1968-04-30
GB998963A (en) 1965-07-21
CH405766A (de) 1966-01-15
DE1498628A1 (de) 1968-11-28

Similar Documents

Publication Publication Date Title
US3150001A (en) Device for use in paper chromatography
US2880697A (en) Coating apparatus
ES219796U (es) Distribuidor de espuma.
DE2605639A1 (de) Vorrichtung zur temperaturregelung bei einem chromatographen
US2551329A (en) Film treating apparatus
US4664526A (en) Process and apparatus for mixing a liquid sample to be analyzed
US2939354A (en) Automatic cut-off apparatus for strip material having photoelectric control means
US2919673A (en) Positioning control means for web striping device
SE7711219L (sv) Rakelanordning for schablontryck
US2659343A (en) Coating liquid applicator
GB1228541A (de)
BR8207871A (pt) Aparelho para espalhar material granular
US2757908A (en) Agitating device
US3635345A (en) Chromatography
GB1388175A (en) Patch applicator apparatus
US2786443A (en) Apparatus for producing light-sensitive coatings on metal sheets
US3169392A (en) Viscometer
GB951356A (en) Improvements in or relating to apparatus for applying a narrow area or band of adhesive along a web
US2729098A (en) Cleaning compound testing apparatus
SU471123A1 (ru) Устройство дл нанесени жидкого покрыти на внутреннюю поверхность труб
US4276777A (en) Apparatus for analysing flowing media
Logothetis et al. A model spotting apparatus for the automatic deposition, on filter paper, of solutions for chromatographic separations
US1724705A (en) Apparatus for cleaning films
SU396668A1 (ru) Капельный дозатор
GB732435A (en) Improvements in or relating to apparatus for applying a fluid coating material to a cylinder