US3406635A - Progressive dilution series apparatus - Google Patents

Progressive dilution series apparatus Download PDF

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Publication number
US3406635A
US3406635A US521204A US52120466A US3406635A US 3406635 A US3406635 A US 3406635A US 521204 A US521204 A US 521204A US 52120466 A US52120466 A US 52120466A US 3406635 A US3406635 A US 3406635A
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United States
Prior art keywords
conduit
pump
motor
diluent
switch
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Expired - Lifetime
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US521204A
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English (en)
Inventor
Tybring Leif
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Leo Pharma AS
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Leo Pharmaceutical Products Ltd AS
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Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/12—Machines, pumps, or pumping installations having flexible working members having peristaltic action
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/712—Feed mechanisms for feeding fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/714—Feed mechanisms for feeding predetermined amounts
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/7176—Feed mechanisms characterised by the means for feeding the components to the mixer using pumps
    • B01F35/717613—Piston pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/80—Forming a predetermined ratio of the substances to be mixed
    • B01F35/83—Forming a predetermined ratio of the substances to be mixed by controlling the ratio of two or more flows, e.g. using flow sensing or flow controlling devices
    • B01F35/831—Forming a predetermined ratio of the substances to be mixed by controlling the ratio of two or more flows, e.g. using flow sensing or flow controlling devices using one or more pump or other dispensing mechanisms for feeding the flows in predetermined proportion, e.g. one of the pumps being driven by one of the flows
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B49/00—Control, e.g. of pump delivery, or pump pressure of, or safety measures for, machines, pumps, or pumping installations, not otherwise provided for, or of interest apart from, groups F04B1/00 - F04B47/00
    • F04B49/02—Stopping, starting, unloading or idling control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples

Definitions

  • the apparatus consists of a rotating or peristaltic working piston pump inserted into a conduit for pumping volumetric quantities of a solution in one or the other direction through a conduit, one end of which forms a connection to a reservoir for diluent while the other end is free to be lowered into a container for basic or diluted solution, the pump being provided with means for terminating the pumping after a measured quantity has been transported towards the end of the conduit to be lowered into the basic or diluted solution and is a predetermined number of times greater than when transporting the liquid towards the reservoir.
  • a progressive-dilution series i.e. a number or series of solutions in which a starting material is present in concentrations that are consecutive members of a geometrical progression. From a substance having the starting concentration A it may thus be desirable to produce a progressive-dilution series, the members of which have the concentrations A, A- 10- A- l0" A- 10" and A-l0 respectively, n being an integer. It is not unusual that progressive-dilution series are required of the order of 5 to 10 members and in some kinds of experimental work the production of such series is a very frequently occurring task.
  • the usual way of producing progressive-dilution series is by using a pipette, which is rather laborious. It consists in extracting with a smaller pipette a predetermined portion of the basic solution, after which a portion of diluent is extracted from a suitable reservoir by means of a pipette, the volume of which is p1 times the volume of the first one, if the rate of dilution is to be p.
  • the measured portion of the basic solution is then mixed with the measured portion of the diluent.
  • a measured portion of the solution thus produced is extracted by means of another pipette of the small size, and another measured amount of the diluent is extracted with the larger pipette, after which the liquids are mixed.
  • the larger pipette used for measuring the diluent may be kept clean, but in this case special care must be taken with regard to emptying it so that the same dilution ratio is obtained each time, which takes a considerable time. Anyhow, for each progressive-dilution series a great number of pipettes is necessary, which pipettes must be cleansed and dried and perhaps sterilized before they can be used anew.
  • the invention aims at providing an apparatus for the production of progressive-dilution series which apparatus 3,406,635 Patented Oct. 22, 1968 is simple and by which the above-mentioned drawbacks are avoided.
  • the apparatus consists in a rotatingly or peristaltically working piston pump inserted into a conduit and designed for pumping volumetrically quantities of a solution in one or the other direction through the conduit.
  • One end of the conduit forms a connection to a reservoir for diluent while the other end is free to be lowered into a container for'basic or diluted solution.
  • the pump is provided with means.
  • the apparatus works in the following way: When the conduit is filled with the diluent and one end of the conduit is in connection with a reservoir for the diluent, and the other end, the free end, is lowered into a container with the basic solution, one or more unit volumes are first sucked into the conduit, whereby a corresponding amount of diluent is simultaneously delivered to the reservoir containing the reserve portion of diluent. Thereupon the pumping terminates and the free end through which the basic solution has been sucked in, is transferred to a container for receiving diluted solution. Now pumping is re versed and the predetermined number of unit volumes is pumped into said container.
  • the basic solution just sucked up leaves the conduit and then a number of unit volumes of the diluent corresponding to the desired total volume.
  • the two liquids mix and before the free end of the conduit is removed from the just formed diluted liquid and by reversing and starting the pump one or more unit volumes of the mixture are sucked into the conduit, whereupon the end of the conduit is transferred to an empty container serving to take up the next dilution step of the series, the pump is reversed and the predetermined number of unit volumes is given off.
  • the process continues in this manner until a series of liquids with increasing degree of dilution has been obtained, the last of which has the highest degree of dilution required for the experiment in view.
  • the apparatus is very Well suited for automatic production of progressive-dilution series as the containers for the diluted liquid may be placed on a conveyor or a turning table, so that another container automatically replaces the former one after each dilution operation.
  • the volume of the conduit is larger than the unit volume.
  • the volume of the conduit including the inserted pump volume was to have the same size as the unit volume, a diffusion of the diluted material into the diluent might occur, whereby said diluent would be contaminated and part of the substance contained in the measured amount of liquid might be lost, whereby the progressive-dilution series would be unreliable.
  • the volume of the conduit is several times that of the unit volume which permits more than one volume to be taken up and consequently a variety of dilution rates can be obtained.
  • only the foremost part of the conduit i.e. the part lying between the diluent and the pump space, is used for taking up a unit volume of the basic liquid or the diluted liquid, or for taking up a few unit volumes.
  • a reliable cleaning of the apparatus during the working is ensured.
  • the apparatus according to the invention is very simple and cheap when the pump is a rotating piston pump of the valveless type, adapted for being driven with alternate directions of rotation, preferably by means of an engine, the direction of rotation of which can be shifted.
  • rotating piston pumps of the valveless type which can be used according to the invention, may be mentioned a rotary vane pump, a gear pump or a flexible-hose pump.
  • a rotary vane pump a gear pump or a flexible-hose pump.
  • a valveless pump By using a valveless pump, however, certain drawbacks are avoided such as faults owing to possible insecure closing of the valves, and that the valves owing to their complicated form may retain impurities or undiluted liquid and thus cause contamination.
  • valves may furthermore give rise to infection from one series to the other.
  • the measured volume is determined by the rotating angle of the rotor and the pumping direction is controlled by the direction of rotation of the rotor. Both are easily adjusted either mechanically or electrically.
  • a flexible-hose pump As compared with other rotating piston pumps of the valveless type a flexible-hose pump has the advantage that the piston moves on the outside of the hose and the closing operation is performed merely by compressing said hose. Thus, it is only the hose which comes into contact with the measured and transported liquid and the pump space and the conduit may form a continuous jointless system with smooth walls which is exceedingly easy to keep clean by rinsing. As mentioned above, when the apparatus is working a constant rinsing with the diluent takes place in connection with each dilution operation, and consequently a pump of the last-mentioned type needs practically no special cleaning.
  • the apparatus according to the invention where the pump is a rotating piston pump of the valveless type, for instance a flexible-hose pump, may preferably be so constructed that the rotor shaft carries or is in constant transmission engagement with a cam wheel having one or more cams serving to discontinue the rotation of the rotor shaft after transportation of a unit volume.
  • a reliable measuring of the unit volumes is obtained.
  • a flexible-hose pump has dead points; by making the moment for cutting off the rotation of the rotor shaft coincide with the moment when the piston passes one of the said dead points, it is possible to obtain an extraordinary exactitude of measuring.
  • the rotor shaft may be in transmission engagement with a second cam wheel by means of a one-way connection, for instance a ratchet connection; said second cam may be provided with one or more cams which serve to discontinue the rotation of the rotor shaft after transportation in the direction towards the container for diluted liquid has taken place, the transmission ratio between the first and the second cam wheel being decided by the dilution ratio and the ratio between the number of cams on the two cam wheels.
  • the apparatus can be worked solely by starting the rotor shaft in one or the other direction, said direction deciding whether the rotor shaft moves in steps corresponding to the measuring of a unit volume or in steps corresponding to the measuring of a total volume which is a predetermined number of times larger than the unit volume.
  • FIG. 1 shows the embodiment in question, seen from above, together with a reservoir for diluent and a container for basic liquid or diluted liquid,
  • FIG. 2 the same apparatus, partly in longitudinal section,
  • FIG. 3 horizontal section through the apparatus in FIG. 2, along the line IIIIII, and
  • FIG. 4 a diagram showing the electric connections in the apparatus according to FIGS. 1-3.
  • the reference number 1 is a house with cylindrical rear wall 2, a plane front and a flat roof 3.
  • a conduit consisting of a tube 6 of elastic pliable material, such as for instance rubber or plastic.
  • a container 30 which may either be a container containing the basic liquid from which a progressive-dilution series is to be produced by means of the diluent in the reservoir 29, or an empty container, in which diluted liquid is to be filled in and from which a measured amount of the thus diluted liquid is again to be sucked up for use in the next step in the progressivedilution series.
  • the rotor 4 is a rotor of the kind known from flexible-hose pumps which on the ends of motor arms carries rollers 5, which during the passage along the cylindrical rear wall 2 compresses the tube 6 so that it closes securely against passage of liquid.
  • the rotor arms 4 with the rollers 5 thus form the pistons of the flexible-hose pump.
  • the rotor is carried by a shaft 9, which is driven by a motor 7 through a gearbox 8.
  • the shaft is supported in the roof 3 by a bearing 13.
  • the rotor shaft 9 carries a spur-gear wheel 11 and a cam wheel 10 which on its circumference has a cam 24.
  • the gear wheel 11 and the cam wheel 10 are mounted on the rotor shaft 9 in fixed connection therewith.
  • the gear wheel 11 is in constant engagement with another gear wheel 17, which is freely rotatable on a shaft 14 parallel with the rotor shaft and mounted in journals 15 and 16 carried on brackets mounted on the walls but not shown in the drawing.
  • the spur-gear wheel 17 carries adjacent to the circumference thereof a pin 19 upon which a ratchet 2 0is rotatable.
  • the ratchet 20 is in engagement with a ratchet wheel 18 mounted on the shaft 14 in fixed connection therewith.
  • the shaft 14 By rotating the gear wheel 17 in one direction the shaft 14 must accordingly follow driven by the ratchet 20, whereas when the gear wheel 17 is turned in the opposite direction, the shaft 14 remains unmoved, kept still by friction means not shown on the drawing.
  • the shaft 14 further carries a worm drive 21 in fixed connection therewith and in engagement with a worm gear 22 carried on a shaft 41.
  • the shaft 41 further carries a cam wheel 23 with a cam 25.
  • the worm gear 22 and the cam wheel 23 are in fixed connection with the shaft 41.
  • a micro-switch 27 of the normally-closed type is mounted on a bracket not shown in such position that it gets in engagement with the cam 25, when the cam wheel 23 is rotated. By such engagement the micro-switch 27 opens.
  • a relay 28 having a coil, an armature and a closing and a shifting contact as later on described, are placed in the housing 1.
  • ratchet and the ratchet wheel are assumed to be oriented in such a manner that the shaft 14 is rotated when the rotor shaft 9 is moved in clockwise direction in FIGS. 1 and 3, i.e. when liquid is pumped from the reservoir 29 to the container 30.
  • the reference number 32 shows a phase line of an electric current distribution network.
  • a glow discharged lamp 33 and a main circuit breaker 44.
  • an electric wire 45 is carried to the switch 27, a connection 46 to the other switch 26, a connection 47 to a foot-operated switch 39 of the normally-open type, which can be closed by pressing the pedal and a further connection 48 to another footoperated switch 40 of the same kind.
  • the relay 28 mentioned above consists of a coil 38, which is activated through the connection 53 and an armature having two contacts 34 and 57, the former closing a contact 35 when activated, the latter breaking a contact 36 and closing a contact 37 when activated.
  • connection 49 is carried to the movable contact 57 from which a connection 50 is carried to the switch 26.
  • a connection 51 is carried to the fixed contact 35 and from the movable armature contact 34 a connection 52 is carried to the switch 26.
  • a connection 58 is carried to the motor 7 and from the fixed contact 37 a connection 55 is carried to another terminal of the motor 7.
  • a third terminal of the motor 7 is connected with the other end 54 of the phase line 32 and the same applies to the spool 38 which is connected to the motor 7 through a connection 54.
  • the motor 7 has current though the connections 45 and 58 if the switch 27 is closed, and in this case the motor continues running clockwise until this switch is opened by the cam 25 of the cam wheel 23 getting in engagement with the microswitch 27.
  • the motor has been stopped and the foot-operated switch 39 is pressed, the coil 38 is activated through the connections 51 and 53 and thus pulls its armature with the movable contacts 34 and 57.
  • the said contacts 34 and 57 are displaced to the right and close the current though the connection 46, the normallyclosed switch 26, the fixed contact 37 and the connection 55, which causes the motor 7 to start and rotate the rotor shaft in anticlockwise direction.
  • the motor continues running until the cam 24 breaks the micro-switch 26.
  • the relay 28 is thereby inactivated. After this the motor is able to restart solely by pressing either the foot switch 39 or the foot switch 40. If the foot switch 39 is pressed, the same will happen as above described, viz. the motor gets current through the connection 55 and accordingly rotates in such a direction that the rotor moves in anti-clockwise direction. Thereby a unit volume is sucked in from the container 30 and the motor stops again after having moved the rotor one turn. This can be repeated a few times, more or less dependent on the volume of the conduit 6, since the amount of liquid sucked in must not 'be allowed to fill the said conduit or get too close to the end of the conduit connected with the diluting agent contained in the reservoir 29.
  • the ratchet 20 turns the ratchet wheel 18 and thus drives the shaft 14 and theAworrn gear 22 and the cam wheel 23, so that the cam 25 gets thrown out of engagement with the micro-switch 27 which accordingly closes.
  • the foot switch 40 can be released without the motor stopping, the motor getting now current through the connection 45, the micro-switch 27 and the connection 58.
  • the apparatus works in the manner intended by activating only the foot contacts 39 and 40. If, for instance, the transmission ratio between the cam wheel 10 and the cam wheel 23 is 1:10, pressing of the foot switch 39 would cause a unit volume of the liquid contained in the container 30 to be sucked in. When, next, the foot switch 40 is pressed totally 10 unit volumes of fluid consisting of 1 volume of the liquid sucked in from the container 30 and 9 volumes diluting agent will be delivered to the container 30. In this manner a progressive-dilution series with the dilution ratio 1:10 is attained. If a progressive dilution series in which the dilution ratio is 1:5, i.e. 2:10 is required, the foot switch 39 must be pressed twice before the foot switch 40 and so on.
  • the volume of the conduit 6 depends on its length and said volume can thus always be so adjusted that it surpasses sufiiciently the number of unit volumes which it can be required to suck into the conduit to obtain a required dilution ratio.
  • the magnitude of the unit volume depends on the sectional area of the conduit 6 and can be adjusted merely by replacing the conduit with another conduit having a different cross-section but the same wall thickness,
  • the velocity of pumping should be so adjusted that a thorough mixing of the liquid to be diluted with the diluting agent is attained at the delivery of the liquid to the container 30.
  • a means for thus adjusting the pumping velocity is to use a suitable velocity of the rotor shaft.-A suitable velocity may for instance be attained by using a gear motor running 60 revolutions per minute, which motor may be a synchronous motor. Hereby it is attained that the motor stops immediately on breaking the current and that the velocity is always the same.
  • a suitable motor may be mentioned the Burgermotor designated RSM provided with gear E.
  • Apparatus for producing progressive-dilution series comprising a volumetric pump inserted in a conduit one end of which is connected to a container for diluting agent, the other end being free and adapted to be lowered into basic fluid or diluted liquid, as the case may be, contained in a container for such liquid, means for activating the said pump in one or the opposite direction and means for automatically discontinuing such pumping after the transportation of a unit volume when the transportation takes place in direction towards the container for diluent, and means for automatically discontinuing transportation after a plurality of unit volumes have been transported when transportation takes place in the direction towards the said free end of the conduit.
  • the pump is a flexible-hose pump the rotor of which is driven by a motor, the direction of rotation of which is shiftable.
  • Apparatus according to claim 4 in which the means for discontinuing the pumping action consist of cams activating electric switches for breaking the circuit of the motor, said cams being carried by cam wheels in transmission engagement with the shaft of the motor of the flexible hose pump, one cam wheel being carried by the rotor shaft and the other by an auxiliary cam wheel shaft being in one-way engagement with the rotor shaft and driven a plurality of times slower than the said rotor shaft.
  • a method of producing a progressive dilution series containing a number of solutions in concentrations that are consecutive members of a geometrical progression which comprises maintaining a supply zone of basic solution from which a progressive dilution series is to be produced, pumping one or more unit volumes of basic solution from said supply zone, simultaneously pumping in the same a corresponding amount of diluent to a reserve zone for diluent, transferring the one or more volumes of basic solution pumped from the supply zone to a receiving 5 zone for receiving diluted solution, now reverse pumping the predetermined number of unit volumes into the receiving zone for diluted solution, then pumping out the two liquids in the last mentioned zone to mix them, collecting the mixed solutions, and then repeating the stated cycle of operations on further amounts of liquids.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Reciprocating Pumps (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Loading And Unloading Of Fuel Tanks Or Ships (AREA)
US521204A 1965-01-18 1966-01-17 Progressive dilution series apparatus Expired - Lifetime US3406635A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK24565AA DK106889C (da) 1965-01-18 1965-01-18 Apparat til fremstilling af fortyndingsrækker.

Publications (1)

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US3406635A true US3406635A (en) 1968-10-22

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US521204A Expired - Lifetime US3406635A (en) 1965-01-18 1966-01-17 Progressive dilution series apparatus

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US (1) US3406635A (da)
DE (1) DE1598707A1 (da)
DK (1) DK106889C (da)
FR (1) FR1508080A (da)
GB (1) GB1076010A (da)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3942915A (en) * 1974-08-05 1976-03-09 Dias, Incorporated Flexible tube pump
US5006122A (en) * 1988-12-02 1991-04-09 The United States Of America As Represented By The Department Of Health And Human Services Tissue transplantation system
EP0607442A4 (en) * 1991-10-08 1994-10-05 Aloka Co Ltd Method of diluting highly viscous liquid.
CN115290421A (zh) * 2022-09-28 2022-11-04 赛默飞世尔（上海）仪器有限公司 稀释设备及稀释方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54102603A (en) * 1978-01-31 1979-08-13 Pilot Pen Co Ltd Undulation pump
JPH0299707A (ja) * 1988-07-09 1990-04-11 Yamaha Motor Co Ltd 潤滑オイル供給装置
US5212952A (en) * 1988-07-09 1993-05-25 Yamaha Hatsudoki Kabushiki Kaisha Compact power supply and lubricant affording device therefor

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2035159A (en) * 1933-09-25 1936-03-24 Henry Louis Eugene Vict Joseph Pump
US2097028A (en) * 1934-03-30 1937-10-26 Major E Gates Fluid controlling device
US2105200A (en) * 1934-04-25 1938-01-11 Hugh G Phelps Surgical pump
US2662666A (en) * 1949-08-04 1953-12-15 Golding Bros Company Inc Injector device
US3055551A (en) * 1959-06-29 1962-09-25 Philip A Johnson Empty-sensing and pump apparatus for liquid vending machines
US3234796A (en) * 1963-04-10 1966-02-15 Lab Line Instr Inc Sample supply apparatus and method
US3277356A (en) * 1963-12-12 1966-10-04 Harold D Kraft Pump arrangement for dispensing predetermined quantities of liquid

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2035159A (en) * 1933-09-25 1936-03-24 Henry Louis Eugene Vict Joseph Pump
US2097028A (en) * 1934-03-30 1937-10-26 Major E Gates Fluid controlling device
US2105200A (en) * 1934-04-25 1938-01-11 Hugh G Phelps Surgical pump
US2662666A (en) * 1949-08-04 1953-12-15 Golding Bros Company Inc Injector device
US3055551A (en) * 1959-06-29 1962-09-25 Philip A Johnson Empty-sensing and pump apparatus for liquid vending machines
US3234796A (en) * 1963-04-10 1966-02-15 Lab Line Instr Inc Sample supply apparatus and method
US3277356A (en) * 1963-12-12 1966-10-04 Harold D Kraft Pump arrangement for dispensing predetermined quantities of liquid

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3942915A (en) * 1974-08-05 1976-03-09 Dias, Incorporated Flexible tube pump
US5006122A (en) * 1988-12-02 1991-04-09 The United States Of America As Represented By The Department Of Health And Human Services Tissue transplantation system
EP0607442A4 (en) * 1991-10-08 1994-10-05 Aloka Co Ltd Method of diluting highly viscous liquid.
CN115290421A (zh) * 2022-09-28 2022-11-04 赛默飞世尔（上海）仪器有限公司 稀释设备及稀释方法

Also Published As

Publication number Publication date
DK106889C (da) 1967-03-28
GB1076010A (en) 1967-07-19
FR1508080A (fr) 1968-01-05
DE1598707A1 (de) 1970-06-18

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