US3554871A - Strip for staining bacteria - Google Patents
Strip for staining bacteria Download PDFInfo
- Publication number
- US3554871A US3554871A US825379A US3554871DA US3554871A US 3554871 A US3554871 A US 3554871A US 825379 A US825379 A US 825379A US 3554871D A US3554871D A US 3554871DA US 3554871 A US3554871 A US 3554871A
- Authority
- US
- United States
- Prior art keywords
- stain
- staining
- bacteria
- solvent
- absorbent material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000010186 staining Methods 0.000 title abstract description 16
- 241000894006 Bacteria Species 0.000 title abstract description 9
- 239000002904 solvent Substances 0.000 abstract description 11
- 239000002250 absorbent Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 6
- 230000002745 absorbent Effects 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000011521 glass Substances 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 229940107698 malachite green Drugs 0.000 description 1
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/805—Test papers
Definitions
- This invention felateS to n1atefialS fof biologica1 1abofa tofy pfocouToS and, Inofe pafticolarly, to stripS fol Staining bacteTia in o1icfoScopic Wofk and the like.
- the pfesent invention pfovideS a papef Stfip contain ing the propef aII1oont of dTied stain foT use in slide Staining.
- the device disdoSed hefoin pfovideS a meanS wheTeby Stain for Staining is appHed to a watef absofbent type of Inatefial and dried, leaving mateTial impregnated with stain and attached to one side of a nonWatef absorbent holding device.
- the abSoTbent InateTial is exposed and a dTop of Solvent which may be vvater is a11OWed to paSs throngh the hole of holeS and thos applied to the suTface of the holding device.
- a dTop of Solvent which may be vvater is a11OWed to paSs throngh the hole of holeS and thos applied to the suTface of the holding device.
- Another object of the invention is to Tovide an im pfoved o1ethod of staining bacteTia on SHdes.
- FIG. 1 is a to vfew of the device accofding to the invention.
- FIG. Z is an fSoIneffic view of the device shown in combination With the SHde.
- FIG. 3 is a bottom vievv of the device accofding to the 1nvention.
- Stain device 10 iS shoWn which may be made of a Inatefia1 in1pefviouS fo water of non-vvateT abSorbent and, fof example, approximately oneSixteenth inch thick.
- the holding nlatefial 10 is Inade of a Sheet of non-vvatef abSoTbent InateTial that haS a hole 11 therein.
- Stained nlatefial nlay be Suppofted on an absofbent paper.
- a handle 13 may be made integral with the holding nlaterial lo, and the nlateTial lZ may be, for exam le, of the ofdef of 0.2 inch thick.
- the device 1" iS Shown dis poSed on a Slide 15 With bactefia 1o on if.
- the SHde may be pfepared in a conventional vvay diSclosed in tho pubHca tion Tefeffed to in fhe Statement of prioT aft hefein.
- the strip Inay be nlade as foHows Example 1 Placing a drop of stain containing the pTopef concen tfation of dye on a stTip of paper and evaporating the vvatef. A depoSit of dye iS left on the papeT.
- a dmp of Watef iS laced on a SHde containing the Hxed bactefia and the papef stTip containing the dye Spot iS placed face down on the dTop of Water.
- EXample II A Sfnall an1ount of gloe iS added to the end sorface of a papef st1ip. A Staining dye (powdered foTm) iS Spfinkled on the glued sufface. (Same procedure used for staining bacteTia on glaSS aS in EXample I).
- Example III The stain powdef is mixed With a Water soluble vehicle, With the viScoSity of peffoleum ieny and capable of hard ening when air d1'ied. ThiS iS then appHed to a papeT Hke surface b S uee in foHowed b air dT i11 to harden, (same proceeduTe uSed fo1 Staining bacteria on glaSS aS in Example I.)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A PROCESS OF STAINING BACTERIA FIXED ON A SLIDE AND DEVICE FOR CARRYING OUT THE PROCESS. THE DEVICE IS MADE OUT OF A NON-ABSORBENT MATERIAL SUPPORTING AN ABSORBENT MATERIAL WHICH HAS BEEN TREATED WITH STAIN AND THEN DRIED. TO STIN THE BACTERIA, EITHER A DROP OF SOLVENT IS PLACED ON THE SLIDE OR SOLVENT IS DROPPED THROUGH A HOLE OR HOLES IN THE NON-ABSORBENT MATERIAL,. THE SOLVENT REDISSOLVES THE STAIN WHICH IS THEN CARRIED DOWN ONTO THE BACTERIA. THE STAIN IS KEPT ON THE BACTERIA FOR AS LONG AS IS NECESSARY TO COMPLETE STAINING. THE STRUCTURE DOES NOT DRY OUT DURING STAINING BECAUSE OF THE PRESENCE OF THE WATER-IMPERVIOUS MATERIAL.
Description
tInited States Patent 3,ss4,871 STRIP FoR STAoIING BACTERIA Anfhony ff. Loffoofo, ffIfo, 1 o., oSSfgnof fo AIneffcon sferiHzef coInpaoy, Efio, 1,., o oofpofoffoo of 1 eno sylvoofa Coonooffon-fn-poff of appHcafion sof. No. S34,78o,
Maf. 1o, 19oo. Thfs appHooffon May 16, 19o9, Sef.
No. 8Z5,379
Inf. CL C1zk 1/Z0 U.S. CL 19s1Z7 1 cloim ABSTRACT oE THE DISCLoSURE A pfoceSS of Staining bactefia xed on a Sl ide and de vice fof Caffying oot the pTocess. The device iS made out of a non-absofbent Inatefia1 suppofting an abSorbont nlateTial which has been treated With stain and thon dried. To Stain the bactefia, eiffler a drop of solvent iS placed on the SHde of Solvent iS dfopped thToogh a hole of holeS in the non-absoTbent ITlateTial. The solvent TediSSolveS the stain 'vvhich is then carried down onto the bacteria. The Stain iS ke t on ffle bacteffa for as long aS is neceSSary to coInplete stoining. The stTuctuTe doeS not dTy out doring Staining becauSe of the pfeSence of the watef-onpef vious TnatefiaL CROSS-REFERENCE To RELATED APPLICATION This application iS a continuationinpaft of patent application, SeT. No. 34 786, filed Maf. 16, 1966, and now abandoned.
STATEMENT OF INVENTION This invention felateS to n1atefialS fof biologica1 1abofa tofy pfocouToS and, Inofe pafticolarly, to stripS fol Staining bacteTia in o1icfoScopic Wofk and the like.
one of the IneSSieSt pfocedufes in biologica1 1aboTa toTies iS the staining of bactefia on glaSS slides. This pfoce dufe uSuaHy fesults in stained handS, clothes, counteTS, and oofS Inffequent uSe of Stain bottles and Stock dye Solution leads to evaporation of solventS, such as wateT, and fesults in SottHng of the Stain. ThiS leadS to an impfopef staining Solution.
The pfesent invention pfovideS a papef Stfip contain ing the propef aII1oont of dTied stain foT use in slide Staining.
The device disdoSed hefoin pfovideS a meanS wheTeby Stain for Staining is appHed to a watef absofbent type of Inatefial and dried, leaving mateTial impregnated with stain and attached to one side of a nonWatef absorbent holding device. A hole of holes aTe foTIned in tho non absofbent holding device on the oppoSite side of the holding device to which the stained Inaterial iS attached. Thus, the abSoTbent InateTial is exposed and a dTop of Solvent Which may be vvater is a11OWed to paSs throngh the hole of holeS and thos applied to the suTface of the holding device. when the absofbent matefial with dffed Stain theTe in comeS in contact With the Solvent, the solvent Win be unifoTInly distfiboted thTo11ghout the InateTia1. The stain Win disSolve in Watef and llpon feaching the saturation P ce oint, a li Ilid containing the stain WiH be Teleasod to the SuTface of slide upon Which the stained nlaterial TeSting.
REFERENCE To PRIOR ART PfeVious typical Staining procedufes in common uSe are deScribed in the Manual of Micfobiological MethodS, McGfaW-Hin Book Co. Inc., New York, N.Y. (197).
0BIECTS oF THE INVENTION It is, accofdingly, an obiect of the invention to pfovide an impfoved device foT Staining bactefia.
Another obiect of the invention is to Tovide an im pfoved o1ethod of staining bacteTia on SHdes.
Yet anothef object of the invention iS to pfovide an ab Sofbent sheet of InateTial having dfied Stain impfegnated thefein SI1ppofted on a holding sheet of nonwateT abSofb ent InateriaL GENERAL DESCRIPTION oF DRAvvINGS FIG. 1 is a to vfew of the device accofding to the invention.
FIG. Z is an fSoIneffic view of the device shown in combination With the SHde. FIG. 3 is a bottom vievv of the device accofding to the 1nvention.
DETAILED DESCRIPTION OF THE DRAvVINGS Now With mofe pafticular Teference to the dfawing, Stain device 10 iS shoWn Which may be made of a Inatefia1 in1pefviouS fo water of non-vvateT abSorbent and, fof example, approximately oneSixteenth inch thick. The holding nlatefial 10 is Inade of a Sheet of non-vvatef abSoTbent InateTial that haS a hole 11 therein. The Watef absorbent mateTial 1Z iS attached to the holding mateTial and the stained mateTial iS expoSed through the hole and indicated at 11. Stained nlatefial nlay be Suppofted on an absofbent paper. A handle 13 may be made integral with the holding nlaterial lo, and the nlateTial lZ may be, for exam le, of the ofdef of 0.2 inch thick. The device 1" iS Shown dis poSed on a Slide 15 With bactefia 1o on if. The SHde may be pfepared in a conventional vvay diSclosed in tho pubHca tion Tefeffed to in fhe Statement of prioT aft hefein.
In carfying out the invention, the strip Inay be nlade as foHows= Example 1 Placing a drop of stain containing the pTopef concen tfation of dye on a stTip of paper and evaporating the vvatef. A depoSit of dye iS left on the papeT. To USe, a dmp of Watef iS laced on a SHde containing the Hxed bactefia and the papef stTip containing the dye Spot iS placed face down on the dTop of Water. The dye diSSolves and StainS the bacteTia.
EXample II A Sfnall an1ount of gloe iS added to the end sorface of a papef st1ip. A Staining dye (powdered foTm) iS Spfinkled on the glued sufface. (Same procedure used for staining bacteTia on glaSS aS in EXample I).
Example III The stain powdef is mixed With a Water soluble vehicle, With the viScoSity of peffoleum ieny and capable of hard ening when air d1'ied. ThiS iS then appHed to a papeT Hke surface b S uee in foHowed b air dT i11 to harden, (same proceduTe uSed fo1 Staining bacteria on glaSS aS in Example I.)
The foTegoing methods wiH eHminate the meSSy problemS in providing stain Strips.
Exam les of stainS Which are in common Usa e are aS foHows: Meth lene Blue, CaTbofuchsin, Malachite Green, Meth l Red, Cr stal violet, Gentian Violet, AniHne Blue and SafTanine.
Exam les of common solvents are: vater, alcohoL acetone, Xylene, phenol and forn1aldehyde.
The embodimentS of the invention in which an exdu- Sive fo eTt or pTiVHe e is claimed is denned aS foHows:
1. A device foT stainin bacteria nxed on a Inicrosco e sHdo com risin a dr vvatef absoTbent stri havin a dried bacteTial stain im Te nated therein and a la er of water iInpeTViouS materia1 With a hole theTein attached to and coverin one Surface of said Stf Said im ervious Inateial having a lateTany extending poItion dening a handle.
Refetences Cifed UNITED STATES PATENTS OTHER REFERENCES ManUal of MicrobiologicaI Methods, McGfaW-Hin Book Co. In New York, p. 21 (19S7).
ALVIN E. TANENHoLT Primar Examiner U.S. CL X.R. 19103.5
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82537969A | 1969-05-16 | 1969-05-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US3554871A true US3554871A (en) | 1971-01-12 |
Family
ID=25243870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US825379A Expired - Lifetime US3554871A (en) | 1969-05-16 | 1969-05-16 | Strip for staining bacteria |
Country Status (1)
| Country | Link |
|---|---|
| US (1) | US3554871A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2436993A1 (en) * | 1978-09-25 | 1980-04-18 | Baylor College Medicine | METHOD AND COMPOSITION FOR THE COLORING AND ANALYSIS OF BACTERIA |
| US4256693A (en) * | 1978-06-06 | 1981-03-17 | Fuji Photo Film Co., Ltd. | Multilayered integral chemical analysis element for the blood |
| EP0173134A1 (en) * | 1984-08-07 | 1986-03-05 | Shionogi & Co., Ltd. | Stain for bacterial flagella |
| US4631174A (en) * | 1978-05-02 | 1986-12-23 | Fuji Photo Film Co., Ltd. | Multilayer chemical analysis member having an outer waterproof layer |
-
1969
- 1969-05-16 US US825379A patent/US3554871A/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4631174A (en) * | 1978-05-02 | 1986-12-23 | Fuji Photo Film Co., Ltd. | Multilayer chemical analysis member having an outer waterproof layer |
| US4256693A (en) * | 1978-06-06 | 1981-03-17 | Fuji Photo Film Co., Ltd. | Multilayered integral chemical analysis element for the blood |
| FR2436993A1 (en) * | 1978-09-25 | 1980-04-18 | Baylor College Medicine | METHOD AND COMPOSITION FOR THE COLORING AND ANALYSIS OF BACTERIA |
| EP0173134A1 (en) * | 1984-08-07 | 1986-03-05 | Shionogi & Co., Ltd. | Stain for bacterial flagella |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| AS | Assignment |
Owner name: MORGAN GUARANTY TRUST COMPANY,NEW YORK Free format text: SECURITY INTEREST;ASSIGNOR:AMERICAN STERILIZER COMPANY, A PA CORP.;REEL/FRAME:004765/0752 Effective date: 19870827 Owner name: MORGAN GUARANTY TRUST COMPANY, 23 WALL STREET, NEW Free format text: SECURITY INTEREST;ASSIGNOR:AMERICAN STERILIZER COMPANY, A PA CORP.;REEL/FRAME:004765/0752 Effective date: 19870827 |
|
| AS | Assignment |
Owner name: AMERICAN STERILIZER COMPANY, ONE MELLON BANK CENTE Free format text: RELEASED BY SECURED PARTY;ASSIGNOR:MORGAN GUARANTY TRUST COMPANY OF NEW YORK;REEL/FRAME:005581/0543 Effective date: 19910130 |