WO1991002798A3 - t-PA-MUTANTE GK1L - Google Patents

t-PA-MUTANTE GK1L Download PDF

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Publication number
WO1991002798A3
WO1991002798A3 PCT/EP1990/001401 EP9001401W WO9102798A3 WO 1991002798 A3 WO1991002798 A3 WO 1991002798A3 EP 9001401 W EP9001401 W EP 9001401W WO 9102798 A3 WO9102798 A3 WO 9102798A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant dna
domains
protein
vectors
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1990/001401
Other languages
English (en)
French (fr)
Other versions
WO1991002798A2 (de
Inventor
Ulrich Weidle
Anne Stern
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to EP90912217A priority Critical patent/EP0440763B1/de
Priority to DE59008764T priority patent/DE59008764D1/de
Publication of WO1991002798A2 publication Critical patent/WO1991002798A2/de
Publication of WO1991002798A3 publication Critical patent/WO1991002798A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Oscillators With Electromechanical Resonators (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Curing Cements, Concrete, And Artificial Stone (AREA)

Abstract

Die Erfindung betrifft eine rekombinante DNA, die für ein Protein mit den Domänen G, K1 und L von t-PA kodiert, bei der die für die Domänen K2 und F des Wildtyp t-PA-Gens kodierenden Sequenzen oder die im Rahmen des genetischen Codes davon abgeleiteten Sequenzen entsprechend der genauen Exon/Intron-Grenzen auf dem t-PA-Gen vollständig deletiert sind. Gegenstand der Erfindung ist auch ein Verfahren zur Herstellung erfindungsgemäßer rekombinanter DNA. Die Erfindung betrifft weiterhin Vektoren, die diese rekombinante DNA enthalten, sowie Zellen, die mit erfindungsgemäßen Vektoren bzw. mit der erfindungsgemäßen DNA transformiert sind. Gegenstand der Erfindung ist weiterhin ein Protein mit fibrinolytischen Eigenschaften durch Expression einer erfindungsgemäßen DNA-Sequenz in geeigneten Wirtszellen, das aus den Aminosäuresequenzen der Domänen G, K1 und L von t-PA in dieser Reihenfolge besteht und gegebenenfalls glykosiliert ist, sowie ein Verfahren zu seiner Herstellung. Schließlich betrifft die Erfindung auch ein fibrinolytisches Mittel, das ein erfindungsgemäßes Protein enthält.
PCT/EP1990/001401 1989-08-23 1990-08-22 t-PA-MUTANTE GK1L Ceased WO1991002798A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP90912217A EP0440763B1 (de) 1989-08-23 1990-08-22 t-PA-MUTANTE GK1L
DE59008764T DE59008764D1 (de) 1989-08-23 1990-08-22 t-PA-MUTANTE GK1L.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3927865A DE3927865A1 (de) 1989-08-23 1989-08-23 T-pa-mutante gk1l
DEP3927865.4 1989-08-23

Publications (2)

Publication Number Publication Date
WO1991002798A2 WO1991002798A2 (de) 1991-03-07
WO1991002798A3 true WO1991002798A3 (de) 1991-04-04

Family

ID=6387718

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1990/001401 Ceased WO1991002798A2 (de) 1989-08-23 1990-08-22 t-PA-MUTANTE GK1L

Country Status (6)

Country Link
US (1) US5223422A (de)
EP (1) EP0440763B1 (de)
JP (1) JPH04500010A (de)
AT (1) ATE120236T1 (de)
DE (2) DE3927865A1 (de)
WO (1) WO1991002798A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2000268170A1 (en) * 2000-09-04 2002-03-22 Hunan Royal Biotech A cell line expressing mutant human tissue-type plasminogen activator, constructing strategy and method of expressed protein preparation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003906A1 (en) * 1985-12-20 1987-07-02 The Upjohn Company Tissue plasminogen activator (tpa) analogs

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003906A1 (en) * 1985-12-20 1987-07-02 The Upjohn Company Tissue plasminogen activator (tpa) analogs

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Febs Letters, Band 189, Nr. 1, September 1985, Published by Elsevier Science Publishers B.V. (Biomedical Division), Federation of European Biochemical Societies, (Amsterdam, NL), H. KAGITANI et al.: "Expression in E. Coli of Finger-Domain Lacking Tissue-Type Plasminogen Activator with High Fibrin Affinity" seiten 145-149, siehe den ganzen artikel *
Gene, Band 79, Nr. 2, 1989, Elsevier Science Publishers B.V. (Biomedical Division), (Amsterdam, NL), A. STERN et al.: "Functional Topology of Human Tissue-Type Plasminogen Activator: Characterization of two Deletion Derivatives and of a Duplication Derivative", seiten 233-344, siehe den ganze artikel *
Gene, Band 87, Nr. 2, 15. Marz 1990, Elsevier Science Publishers B.V. (Biomedical Division), (Amsterdam, NL), A. STERN et al.: "Kringle 1 Domain of Human Tissue-Type Plasminogen Activator is a Functional Module Mediating Fibrinogen-Stimulated Plasminogenolytic Activity", seiten 305-308, siehe den ganze artikel *

Also Published As

Publication number Publication date
EP0440763B1 (de) 1995-03-22
DE3927865A1 (de) 1991-02-28
ATE120236T1 (de) 1995-04-15
JPH0571228B2 (de) 1993-10-06
EP0440763A1 (de) 1991-08-14
WO1991002798A2 (de) 1991-03-07
JPH04500010A (ja) 1992-01-09
US5223422A (en) 1993-06-29
DE59008764D1 (de) 1995-04-27

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