WO1992010754A1 - Procede et appareils d'analyse - Google Patents

Procede et appareils d'analyse Download PDF

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Publication number
WO1992010754A1
WO1992010754A1 PCT/GB1991/002181 GB9102181W WO9210754A1 WO 1992010754 A1 WO1992010754 A1 WO 1992010754A1 GB 9102181 W GB9102181 W GB 9102181W WO 9210754 A1 WO9210754 A1 WO 9210754A1
Authority
WO
WIPO (PCT)
Prior art keywords
receptacle
reaction
circulation
sample
platform
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1991/002181
Other languages
English (en)
Inventor
Aled Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Products Ltd
Original Assignee
Euro DPC Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB909026706A external-priority patent/GB9026706D0/en
Application filed by Euro DPC Ltd filed Critical Euro DPC Ltd
Publication of WO1992010754A1 publication Critical patent/WO1992010754A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L99/00—Subject matter not provided for in other groups of this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/27—Mixing the contents of independent containers, e.g. test tubes the vibrations being caused by electromagnets
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/4905—Determining clotting time of blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00524—Mixing by agitating sample carrier

Definitions

  • the present invention is concerned with an analytical method and apparatus, useful, inter alia, for inducing and monitoring coagulation and other reactions.
  • the apparatus according to the present invention is particularly applicable for studying the coagulation of human blood (although it can be used for any other reaction involving viscosity change or where thorough circulation and mixing is required in an open- topped receptacle) . It is often necessary to detect the precise time at which coagulation occurs following the addition of certain substances to blood.
  • the analysis generally comprises agitation of the sample whilst simultaneously monitoring its viscosity. It is known to shake reaction vessels (such as microplates) during an analytical procedure and subsequently monitor the extent of the reaction. However, with such apparatus it is difficult, or impossible, to carry out reproducible sample agitation.
  • analytical apparatus comprising: a) a reagent package comprising at least one open-topped receptacle arranged for the reaction therein of a sample and a reagent; b) support means for said package comprising a platform having, around a peripheral region thereof, a plurality of magnetisable elements; c) a plurality of electromagnets, each being spaced from and adjacent to a corresponding magnetisable element, and being arranged to produce controlled oscillatory movement of said support means and thereby inducing circulation in a liquid present in the or each said receptacle about a respective vertical axis in the or each said receptacle; and d) means for monitoring the extent of reaction between said sample and said reagent during induction of said circulation.
  • the magnetisable elements are located at substantially equi-distant positions around the peripheral regio of the platform.
  • the magnetisable elements are of mil steel or other ferromagnetic material.
  • each electromagne is preferably switched sequentially (using known stepper motor technology) so as to cause attraction between a respective electromagnet and its corresponding adjacent magnetisable element.
  • the frequency, amplitude and direction of this sequential "attraction process" can be varied and thus a vortex can be induced in the liquid in each receptacle, rotating around a vertical axis.
  • the monitoring means can be arranged to follow separately the reaction within a single receptacle or simultaneously the reactions within a plurality of receptacles.
  • Suitable monitori means include any one of the following:- a) a video camera arranged to view a plurality of receptacles simultaneous1y; b) an optical device such as a photodiode arranged to view ea receptacle individually; c) a scanning device arranged to view receptacles in turn (e. using optical fibres or a beam splitter); or d) electromagnetic means to detect a change in flux linkage with said object.
  • a method comprising:- a) locating within at least one open topped receptacle, disposed on a platform; a reaction mixture comprising a sample and reagent capable of undergoing a coagulation reaction with said sample; b) electromagnetically inducing circulation of said reaction mixture in the or each said receptacle about a respective vertical axis by controlled oscillatory movement of said platform; and c) monitoring the extent of reaction between said sample and said reagent during induction of said circulation.
  • FIG. 1 is a plan view of apparatus according to the invention
  • Figure 2 is a vertical section along plane A-A of Figure 1
  • Figure 3 is an enlarged plan view of a single receptacle used in the apparatus of Figure 1.
  • apparatus generally designated 1 comprising a base portion 2, a movable support 3 an an uppermost platform 4.
  • Central axis 5 attaches the upper face of base portion 2 to support 3, whereas support legs 6 attach th upper face of support 3 to the lower face of platform 4.
  • Support legs 6 and magnetisable elements 7 occupy alternating positions around the periphery of support 3.
  • Remote from suppor 3 and adjacent to each element 7 are separate electromagnets 8.
  • Each electromagnet 8 sequentially attracts the corresponding adjacent magnetisable element 7 to produce oscillatory vibrational movement of platform 4.
  • Microplate 9 lies on the surface of platform 4 and comprises an array (such as an 8 x 12 array) of open-topped microwells 10.
  • FIG. 3 there is shown the circulation of the reagents within each microwell 10 as a result of the oscillatory movement of platform 4; this is anticlockwise in the illustrated embodiment, but it is of course possible to arrange for the circulation to be clockwise.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Ecology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electromagnetism (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'appareil comprend: a) un ensemble contenant un réactif comprenant au moins un récipient ouvert au sommet; b) une structure de support destiné à l'ensemble et se composant d'un plateau pourvu d'une multiplicité d'éléments pouvant être magnétisés installés sur son pourtour; c) une multiplicité d'électro-aimants, chacun étant espacé par rapport à un élément correspondant pouvant être magnétisé et adjacent à celui-ci, et étant agencé de façon à produire un mouvement oscillatoire régulé dans la structure de support afin de faire circuler un mélange de réaction présent dans le récipient ou dans chaque récipient, et d) un dispositif tel qu'une caméra vidéo servant à surveiller l'étendue de la réaction (telle que la réaction de coagulation) lors de la mise en circulation. Le procédé consiste à placer un mélange de réaction dans le récipient ou dans chaque récipient, à produire de manière électromagnétique un mouvement de circulation du mélange et à surveiller l'étendue de la réaction pendant la mise en circulation.
PCT/GB1991/002181 1990-12-07 1991-12-09 Procede et appareils d'analyse Ceased WO1992010754A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9026706.3 1990-12-07
GB909026706A GB9026706D0 (en) 1990-12-07 1990-12-07 Apparatus and method for coagulation analysis
GB9122053.3 1991-10-17
GB9122053A GB2254423A (en) 1990-12-07 1991-10-17 Analytical apparatus with agitation of samples

Publications (1)

Publication Number Publication Date
WO1992010754A1 true WO1992010754A1 (fr) 1992-06-25

Family

ID=26298077

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1991/002181 Ceased WO1992010754A1 (fr) 1990-12-07 1991-12-09 Procede et appareils d'analyse

Country Status (3)

Country Link
AU (1) AU9037291A (fr)
GB (1) GB2254423A (fr)
WO (1) WO1992010754A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005098397A1 (fr) * 2004-04-02 2005-10-20 Rwth Aachen Procede et dispositif permettant d'enregistrer des parametres de processus de liquides reactionnels dans plusieurs microreacteurs agites
DE102005051012B3 (de) * 2005-10-25 2007-04-19 Gesellschaft zur Förderung der Analytischen Wissenschaften e.V. Vorrichtung zur Handhabung von biologischen Zellen bzw. Proben
CN101213012B (zh) * 2005-05-04 2012-02-15 泰肯贸易股份公司 用来移动液体容器的装置和方法
DE102016116377A1 (de) * 2016-09-01 2018-03-01 Rheinisch-Westfälisch Technische Hochschule (RWTH) Aachen Verfahren und Vorrichtung zur Erfassung von Prozessparametern in Flüssigkulturen

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2277043B (en) * 1993-04-13 1996-10-30 David John Fletcher Orbital shaking attachment
DE20018633U1 (de) * 2000-10-31 2001-01-18 Herz, Helmut, Dr.-Ing., 85764 Oberschleißheim Schüttelgeräte für Probengefäße

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3650698A (en) * 1969-12-04 1972-03-21 Technicon Corp Apparatus for the automatic determination of the coagulation, aggregation and or flocculation, or the like, rates of fluids, and novel reaction intensifying agent for use therewith
EP0063744A2 (fr) * 1981-04-18 1982-11-03 Forschungszentrum Jülich Gmbh Procédé et appareil pour la culture en série de micro-organismes
EP0308994A2 (fr) * 1983-06-15 1989-03-29 GOROG, Peter Méthode pour pomper du sang
EP0325874A1 (fr) * 1987-12-30 1989-08-02 Serbio Procédé et dispositif pour déterminer le temps de modification de l'état physique d'un milieu fluide

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3324628A (en) * 1964-09-08 1967-06-13 Scientific Industries Preparative treatment of samples for subsequent processing in a gas chromatograph
GB2057903A (en) * 1979-09-05 1981-04-08 Fowler M W Device for shaking vessels

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3650698A (en) * 1969-12-04 1972-03-21 Technicon Corp Apparatus for the automatic determination of the coagulation, aggregation and or flocculation, or the like, rates of fluids, and novel reaction intensifying agent for use therewith
EP0063744A2 (fr) * 1981-04-18 1982-11-03 Forschungszentrum Jülich Gmbh Procédé et appareil pour la culture en série de micro-organismes
EP0308994A2 (fr) * 1983-06-15 1989-03-29 GOROG, Peter Méthode pour pomper du sang
EP0325874A1 (fr) * 1987-12-30 1989-08-02 Serbio Procédé et dispositif pour déterminer le temps de modification de l'état physique d'un milieu fluide

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Dynatech Laboratories Ltd product information *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005098397A1 (fr) * 2004-04-02 2005-10-20 Rwth Aachen Procede et dispositif permettant d'enregistrer des parametres de processus de liquides reactionnels dans plusieurs microreacteurs agites
US8268632B2 (en) * 2004-04-02 2012-09-18 Rwth Aachen Method and device for recording process paramaters of reaction fluids in several agitated microreactors
CN101213012B (zh) * 2005-05-04 2012-02-15 泰肯贸易股份公司 用来移动液体容器的装置和方法
DE102005051012B3 (de) * 2005-10-25 2007-04-19 Gesellschaft zur Förderung der Analytischen Wissenschaften e.V. Vorrichtung zur Handhabung von biologischen Zellen bzw. Proben
DE102016116377A1 (de) * 2016-09-01 2018-03-01 Rheinisch-Westfälisch Technische Hochschule (RWTH) Aachen Verfahren und Vorrichtung zur Erfassung von Prozessparametern in Flüssigkulturen
US11635381B2 (en) 2016-09-01 2023-04-25 Rheinisch-Westfälisch Technische Hochschule (RWTH) Aachen Method and device for measuring process parameters in liquid cultures

Also Published As

Publication number Publication date
GB2254423A (en) 1992-10-07
GB9122053D0 (en) 1991-11-27
AU9037291A (en) 1992-07-08

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