WO1992013229A3 - Procede d'analyse par electrophorese capillaire avec detection par fluorescence - Google Patents

Procede d'analyse par electrophorese capillaire avec detection par fluorescence Download PDF

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Publication number
WO1992013229A3
WO1992013229A3 PCT/FR1992/000011 FR9200011W WO9213229A3 WO 1992013229 A3 WO1992013229 A3 WO 1992013229A3 FR 9200011 W FR9200011 W FR 9200011W WO 9213229 A3 WO9213229 A3 WO 9213229A3
Authority
WO
WIPO (PCT)
Prior art keywords
capillary
laser beam
analysis method
fluorescence
fluorescence detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR1992/000011
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English (en)
Other versions
WO1992013229A2 (fr
Inventor
Luis Hernandez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Europhor SA
Original Assignee
Europhor SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europhor SA filed Critical Europhor SA
Publication of WO1992013229A2 publication Critical patent/WO1992013229A2/fr
Publication of WO1992013229A3 publication Critical patent/WO1992013229A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Procédé d'analyse par électrophorèse capillaire avec détection par fluorescence, dans lequel on fait passer dans un capillaire d'électrophorèse de diamètre intérieur inférieur ou égal à 100 νm un échantillon contenant les produits à analyser marqués au besoin par un agent de fluorescence. On utilise pour exciter la fluorescence un faisceau laser focalisé sur le capillaire par un dispositif de concentration de la lumière et on détecte la fluorescence de manière colinéaire, soit dans l'axe d'illumination par le faisceau laser et du même côté du capillaire, à l'aide du dispositif de concentration de la lumière utilisé pour focaliser le faisceau laser et d'un photodétecteur. Ce procédé permet un grand nombre d'applications, notamment en biologie moléculaire et dans le domaine pharmaceutique, ainsi que pour l'analyse de traces d'ions.
PCT/FR1992/000011 1991-01-08 1992-01-08 Procede d'analyse par electrophorese capillaire avec detection par fluorescence Ceased WO1992013229A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9100144A FR2671407B1 (fr) 1991-01-08 1991-01-08 Procede d'analyse par electrophorese capillaire avec detection par fluorescence, et dispositifs de mise en óoeuvre.
FR91/00144 1991-01-08

Publications (2)

Publication Number Publication Date
WO1992013229A2 WO1992013229A2 (fr) 1992-08-06
WO1992013229A3 true WO1992013229A3 (fr) 1992-10-15

Family

ID=9408509

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1992/000011 Ceased WO1992013229A2 (fr) 1991-01-08 1992-01-08 Procede d'analyse par electrophorese capillaire avec detection par fluorescence

Country Status (2)

Country Link
FR (1) FR2671407B1 (fr)
WO (1) WO1992013229A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5348633A (en) * 1993-01-22 1994-09-20 Northeastern University Method for quantitating trace amounts of an analyte in a sample by affinity capillary electrophoresis
JP3727031B2 (ja) * 1994-02-07 2005-12-14 アプレラ コーポレイション ポリヌクレオチド分析のための螢光に基づく電気泳動システム
DE4411998A1 (de) * 1994-04-08 1995-10-19 Bioquant Gmbh Unternehmen Fuer Kollektor für optische Strahlung
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
DE10140680A1 (de) * 2001-08-24 2003-03-06 Bayer Ag Spektroskopisches Testsystem auf Basis von Mikrokapillaren
WO2004055492A2 (fr) * 2002-12-13 2004-07-01 Aclara Biosciences, Inc. Commande en boucle fermee de processus electrocinetiques dans des dispositifs microfluidiques bases sur des lectures optiques
CN102879365A (zh) * 2012-09-21 2013-01-16 常州大学 一种毛细管电泳荧光检测装置
CN106596626B (zh) * 2016-11-29 2019-04-09 武汉大学 瞬态荧光测量材料热扩散率的方法及装置
CN108593748B (zh) * 2018-01-26 2024-04-30 南京溯远基因科技有限公司 毛细管及dna测序仪

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2558262A1 (fr) * 1984-01-16 1985-07-19 California Inst Of Techn Procede et systeme pour l'analyse par electrophorese de fragments d'adn obtenus par sequencage
EP0216600A2 (fr) * 1985-09-18 1987-04-01 The Board Of Trustees Of The Leland Stanford Junior University Fluorescence par excitation laser pour la détection de séparation électrocinétique
EP0442315A1 (fr) * 1990-01-29 1991-08-21 Waters Investments Limited Procédé de séparation d'espèces ioniques par électrophorèse capillaire

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2558262A1 (fr) * 1984-01-16 1985-07-19 California Inst Of Techn Procede et systeme pour l'analyse par electrophorese de fragments d'adn obtenus par sequencage
EP0216600A2 (fr) * 1985-09-18 1987-04-01 The Board Of Trustees Of The Leland Stanford Junior University Fluorescence par excitation laser pour la détection de séparation électrocinétique
EP0442315A1 (fr) * 1990-01-29 1991-08-21 Waters Investments Limited Procédé de séparation d'espèces ioniques par électrophorèse capillaire

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANALYTICAL CHEMISTRY, vol. 53, no. 8, juillet 1981, pages 1298-1302, J.W. JORGENSON et al.: "Zone Electrophoresis in Open-Tubular Glass Capillaries", abrégé *
ANALYTICAL CHEMISTRY, vol. 62, no. 12, juin 1990, pages 403R-414R, California, US; W.G. KUHR: "Capillary Electrophoresis", page 406R,407R *
APPLIED SPECTROSCOPY, vol. 44, no. 5, 1990, Tennessee, US, Y.-F. CHENG et al.: "Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE), abrégé *
JOURNAL OF CHROMATOGRAPHY, vol. 502, 1990, pages 247-255, Amsterdam, NL, L. HERNANDEZ et al.: "Detection and quantification of capillary electrophoresis zones by fluorescence microscopy", abrégé, pages 248,255, figures 1,2 *

Also Published As

Publication number Publication date
FR2671407A1 (fr) 1992-07-10
FR2671407B1 (fr) 1994-08-05
WO1992013229A2 (fr) 1992-08-06

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