WO1998050580A3 - Verfahren zur quantitativen bestimmung der anzahl von transgenkopien in gentechnisch veränderten zellen - Google Patents

Verfahren zur quantitativen bestimmung der anzahl von transgenkopien in gentechnisch veränderten zellen Download PDF

Info

Publication number
WO1998050580A3
WO1998050580A3 PCT/DE1998/001190 DE9801190W WO9850580A3 WO 1998050580 A3 WO1998050580 A3 WO 1998050580A3 DE 9801190 W DE9801190 W DE 9801190W WO 9850580 A3 WO9850580 A3 WO 9850580A3
Authority
WO
WIPO (PCT)
Prior art keywords
transmuted
copies
determining
genetically manipulated
manipulated cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE1998/001190
Other languages
English (en)
French (fr)
Other versions
WO1998050580A2 (de
Inventor
Christoph Fiehn
Nina Wettschureck
Alexandra Krauthoff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU82086/98A priority Critical patent/AU8208698A/en
Publication of WO1998050580A2 publication Critical patent/WO1998050580A2/de
Publication of WO1998050580A3 publication Critical patent/WO1998050580A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Beschrieben ist ein Verfahren zur quantitativen Bestimmung der Anzahl von Transgenkopien in gentechnisch veränderten Zellen. Weiterhin ist ein Verfahren zur quantitativen Bestimmung der Anzahl von gentechnisch veränderten Zellen anhand der Anzahl der Transgenkopien beschrieben.
PCT/DE1998/001190 1997-05-02 1998-04-28 Verfahren zur quantitativen bestimmung der anzahl von transgenkopien in gentechnisch veränderten zellen Ceased WO1998050580A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU82086/98A AU8208698A (en) 1997-05-02 1998-04-28 Method for determining the number of transmuted copies in genetically manipul ated cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19718705A DE19718705A1 (de) 1997-05-02 1997-05-02 Verfahren zur quantitativen Bestimmung der Anzahl von Transgenkopien in gentechnisch veränderten Zellen
DE19718705.6 1997-05-02

Publications (2)

Publication Number Publication Date
WO1998050580A2 WO1998050580A2 (de) 1998-11-12
WO1998050580A3 true WO1998050580A3 (de) 1999-02-04

Family

ID=7828519

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/001190 Ceased WO1998050580A2 (de) 1997-05-02 1998-04-28 Verfahren zur quantitativen bestimmung der anzahl von transgenkopien in gentechnisch veränderten zellen

Country Status (3)

Country Link
AU (1) AU8208698A (de)
DE (1) DE19718705A1 (de)
WO (1) WO1998050580A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002537856A (ja) * 1999-03-05 2002-11-12 トランジェーヌ ヌクレオチド断片、プローブ、プライマー、試薬、及びｐＢＲ３２２の複製起点由来のヌクレオチド配列を検出する方法
HU0600159D0 (en) * 2006-02-23 2006-04-28 Mta Szegedi Biolog Koezpont Kvantitative pcr
DE102006021257A1 (de) * 2006-04-28 2007-11-08 Eberhard-Karls-Universität Tübingen Universitätsklinikum Nachweis von transgener DNA (t DNA)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (en) * 1993-04-06 1994-10-13 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (en) * 1993-04-06 1994-10-13 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 1983, BECK ET AL.: "TRANSPOSON Tn5 FRAGMENT ENCODING NEOMYCIN AND KANAMYCIN RESISTANCE AND A FRAGMENT OF THE READING FRAME OF A FURTHER PROTEIN", XP002080465 *
HO S N ET AL: "SITE-DIRECTED MUTAGENESIS BY OVERLAP EXTENSION USING THE POLYMERASE CHAIN REACTION", GENE, vol. 77, no. 1, 1 January 1989 (1989-01-01), pages 51 - 59, XP000272761 *
MORGAN R A ET AL: "HUMAN GENE THERAPY", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 62, 1993, pages 191 - 217, XP002031972 *
REISCHL U ET AL: "QUANTITATIVE PCR A SURVEY OF THE PRESENT TECHNOLOGY", MOLECULAR BIOTECHNOLOGY, vol. 3, 1995, pages 55 - 71, XP000600241 *

Also Published As

Publication number Publication date
WO1998050580A2 (de) 1998-11-12
AU8208698A (en) 1998-11-27
DE19718705A1 (de) 1998-11-05

Similar Documents

Publication Publication Date Title
AU7008896A (en) A method to increase the density of antigen on antigen presenting cells
HUP9903761A3 (en) Method for the fabrication of electrochemical cells
AU5739996A (en) Method for detection of neoplastic cells
AU6245498A (en) Method for separating cells
AU8724598A (en) System and method for automatically detecting malignant cells and cells having malignancy-associated changes
AU1674795A (en) Method of automated signature verification
AU7760791A (en) Method for plaza deck construction
AU6837796A (en) Fuel cell
AU6489496A (en) Cell adhesion inhibitors
AU2310392A (en) Method for identifying t cells disease involved in autoimmune disease
AU6305194A (en) Method for making alkali-soluble emulsion copolymer
AU6846194A (en) Novel endoglucanase enzyme
AU3635695A (en) Methods for genetically modifying hematopoietic stem cells
AU5116596A (en) Cell culture method
EP0773993A4 (de) Gentechnisch veränderte schweine-zellen
AU7252298A (en) A method and system for simulating the solar cycle
AU3399295A (en) Method of detecting autoantibody present in the serum of reumatic
AU1025897A (en) Solar cell contacting scheme
AU6519696A (en) A method for determining the integrity of nucleic acid
WO1998050580A3 (de) Verfahren zur quantitativen bestimmung der anzahl von transgenkopien in gentechnisch veränderten zellen
AU6083898A (en) Method for the manufacture of photovoltaic cell
AU3326597A (en) Immunoassay method
AU2616095A (en) Method for introducing foreign matter into higher eukaryotic cells
AU8944791A (en) Electrolytic cell for electrolytic processes in which gases are developed or consumed, and a method of manufacturing the cell
AU6736598A (en) Methods for selecting cells and their uses

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: JP

Ref document number: 1998547607

Format of ref document f/p: F

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA