WO1999036517A3 - Verfahren zur selektion von ribozymen, die ribonucleinsäuren in tnl9900033'-oh-gruppen kovalent modifizieren können - Google Patents

Verfahren zur selektion von ribozymen, die ribonucleinsäuren in tnl9900033'-oh-gruppen kovalent modifizieren können Download PDF

Info

Publication number
WO1999036517A3
WO1999036517A3 PCT/EP1999/000181 EP9900181W WO9936517A3 WO 1999036517 A3 WO1999036517 A3 WO 1999036517A3 EP 9900181 W EP9900181 W EP 9900181W WO 9936517 A3 WO9936517 A3 WO 9936517A3
Authority
WO
WIPO (PCT)
Prior art keywords
ribozymes
ribonucleic acids
trans
groups
covalently modifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1999/000181
Other languages
English (en)
French (fr)
Other versions
WO1999036517A2 (de
Inventor
Andreas Jenne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to EP99907369A priority Critical patent/EP1060246A2/de
Publication of WO1999036517A2 publication Critical patent/WO1999036517A2/de
Publication of WO1999036517A3 publication Critical patent/WO1999036517A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Die vorliegende Erfindung betrifft ein in vitro-Selektionsverfahren, mit dem Ribozyme selektiert werden können, die 2'-OH-Gruppen von Ribonucleinsäuren in trans kovalent modifizieren können. Die vorliegende Erfindung betrifft außerdem Ribozyme, die durch dieses Verfahren erhältlich sind. Außerdem betrifft die vorliegende Erfindung diese enthaltende Arzneimittel, die vorzugsweise zur Hemmung der Genexpression - beispielsweise in der Gentherapie - verwendet werden können. Die erfingungsgemäßen Ribozyme können darüber hinaus zur Herstellung von Muteinen und nucleaseresistenten Ribonucleinsäuren - beispielsweise ribonucleaseresistenten 'Antisense'-Oligonucleotide - verwendet werden.
PCT/EP1999/000181 1998-01-14 1999-01-14 Verfahren zur selektion von ribozymen, die ribonucleinsäuren in tnl9900033'-oh-gruppen kovalent modifizieren können Ceased WO1999036517A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP99907369A EP1060246A2 (de) 1998-01-14 1999-01-14 Verfahren zur selektion von ribozymen, die ribonucleinsäuren in trans an 2' -oh-gruppen kovalent modifizieren können

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19801153.9 1998-01-14
DE1998101153 DE19801153A1 (de) 1998-01-14 1998-01-14 Verfahren zur Selektion von Ribozymen, die Ribonucleinsäuren in trans an 2'-OH-Gruppen kovalent modifizieren können und durch dieses Verfahren erhältliche Ribozyme

Publications (2)

Publication Number Publication Date
WO1999036517A2 WO1999036517A2 (de) 1999-07-22
WO1999036517A3 true WO1999036517A3 (de) 1999-09-23

Family

ID=7854593

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000181 Ceased WO1999036517A2 (de) 1998-01-14 1999-01-14 Verfahren zur selektion von ribozymen, die ribonucleinsäuren in tnl9900033'-oh-gruppen kovalent modifizieren können

Country Status (3)

Country Link
EP (1) EP1060246A2 (de)
DE (1) DE19801153A1 (de)
WO (1) WO1999036517A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1177281B1 (de) 1999-04-30 2009-01-21 Cyclops Genome Sciences Limited Isolierung von nukleinsäuren
JP4745577B2 (ja) * 1999-11-24 2011-08-10 ザ リサーチ ファウンデイション オブ ステイト ユニバーシティー オブ ニューヨーク アミノアシル化活性を有した触媒ＲＮＡｓ
ATE437946T1 (de) * 2002-02-15 2009-08-15 Univ New York State Res Found Ribozyme mit breiter trna-aminoacylierungswirkung
WO2007066627A1 (ja) * 2005-12-06 2007-06-14 The University Of Tokyo 多目的アシル化触媒とその用途

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
HAGER, A. ET AL.: "Ribozymes : aiming at RNA replication and protein synthesis", CHEMISTRY AND BIOLOGY., vol. 3, September 1996 (1996-09-01), pages 717 - 725, XP002104957, ISSN: 1074-5521 *
IBBA M: "STRATEGIES FOR IN-VITRO AND IN-VIVO TRANSLATION WITH NON-NATURAL AMINO-ACIDS", BIOTECHNOLOGY & GENETIC ENGINEERING REVIEWS, (1996) VOL. 13, PP. 197-216., XP002109693 *
ILLANGASEKARE M ET AL: "AMINOACYL-RNA SYNTHESIS CATALYZED BY AN RNA", SCIENCE, vol. 267, 3 February 1995 (1995-02-03), pages 643 - 647, XP002044710, ISSN: 0036-8075 *
JENNE A ET AL: "A novel ribozyme with ester transferase activity", CHEMISTRY & BIOLOGY, VOL. 5, NO. 1, PP. 23-34., 15 January 1998 (1998-01-15), XP002109695 *
LORSCH J R ET AL: "IN VITRO EVOLUTION OF NEW RIBOZYMES WITH POLYNUCLEOTIDE KINASE ACTIVITY", NATURE, vol. 371, 1 September 1994 (1994-09-01), pages 31 - 36, XP002044711, ISSN: 0028-0836 *
PAN, T.: "Novel and variant ribozymes obtained through in vitro selection", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 1, 1997, pages 17 - 25, XP002109694 *

Also Published As

Publication number Publication date
WO1999036517A2 (de) 1999-07-22
DE19801153A1 (de) 1999-07-15
EP1060246A2 (de) 2000-12-20

Similar Documents

Publication Publication Date Title
EP1340765A3 (de) Invertierte Chimäre und Hybrid-Oligonukleotide
EP2292771A3 (de) Sense mRNA Therapie
AU6617898A (en) An antisense oligonucleotide preparation method
EP1975172A3 (de) Hochaffine TGFß-Nukleinsäureliganden und -inhibitoren
CA2296769A1 (en) Modified dorsal tissue affecting factor and compositions
CA2265917A1 (en) Method for using oligonucleotides having modified cpg dinucleosides
AU5959396A (en) Antisense oligonucleotide modulation of raf gene expression
WO1996019568A3 (en) Stabilized ribozyme analogs
CA2359180A1 (en) Method and medicament for inhibiting the expression of a given gene
WO1999046372A3 (en) Ribozymes capable of inhibiting the expression of the ccr5 receptor
AU1606897A (en) Thymidine kinase variants, corresponding nucleic acid sequences and their use in gene therapy
EP1741780A3 (de) Ologonukleotidliganden mit höher Affinität für Wachstumsfaktoren
CA2191795A1 (en) Antisense oligonucleotide modulation of raf gene expression
EP0804563A4 (de) Konstrukt zur bildung von antisense oligonucleotiden
AU6589094A (en) 7-deazapurine modified oligonucleotides
CA2086672A1 (en) Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
WO1997007784A3 (en) LIPOSOMAL PHOSPHODIESTER, PHOSPHOROTHIOATE, AND p-ETHOXY OLIGONUCLEOTIDES
HUP9801216A3 (en) Dna molecules, preparation thereof and use thereof in gene therapy
EP1690544A3 (de) Pharmazeutische Zusammensetzungen mit einem Decoy und Verfahren zu ihrer Verwendung
HUT66458A (en) Oligonucleotide and rybozyme analogoues with terminal 3'-3' or 5'-5'bonds, and process for preparing pharmaceutical preparations containing them
AU1062997A (en) Novel polymer supports for nucleic acid synthesis
AU3295700A (en) Oligonucleotides containing an antisense sequence stabilised by a secondary structure and pharmaceutical compositions containing same
WO1997009420A3 (en) Dna sequences coding for a human metalloproteinase and variants thereof
WO1999036517A3 (de) Verfahren zur selektion von ribozymen, die ribonucleinsäuren in tnl9900033'-oh-gruppen kovalent modifizieren können
PT1006200E (pt) Aplicações com, e métodos para produzir, ligações cruzadas intercadeia seleccionadas, em ácidos nucleicos

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): IL US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): IL US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1999907369

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 09600330

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 1999907369

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999907369

Country of ref document: EP