WO1999041607A3 - Fluoreszenz-energie-transfer für die aufklärung der 3d-struktur von biomakromolekülen - Google Patents

Fluoreszenz-energie-transfer für die aufklärung der 3d-struktur von biomakromolekülen Download PDF

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Publication number
WO1999041607A3
WO1999041607A3 PCT/EP1999/001008 EP9901008W WO9941607A3 WO 1999041607 A3 WO1999041607 A3 WO 1999041607A3 EP 9901008 W EP9901008 W EP 9901008W WO 9941607 A3 WO9941607 A3 WO 9941607A3
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WO
WIPO (PCT)
Prior art keywords
macromolecule
cross
photoactivatable
groups
linking
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1999/001008
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English (en)
French (fr)
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WO1999041607A2 (de
Inventor
Daniel Hoffmann
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GMD Forschungszentrum Informationstechnik GmbH
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GMD Forschungszentrum Informationstechnik GmbH
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Application filed by GMD Forschungszentrum Informationstechnik GmbH filed Critical GMD Forschungszentrum Informationstechnik GmbH
Priority to JP2000531739A priority Critical patent/JP2002503810A/ja
Priority to US09/622,161 priority patent/US6713256B1/en
Priority to EP99911675A priority patent/EP1057020A2/de
Publication of WO1999041607A2 publication Critical patent/WO1999041607A2/de
Publication of WO1999041607A3 publication Critical patent/WO1999041607A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/973—Simultaneous determination of more than one analyte
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/819—Multifunctional antigen or antibody
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Verfahren zur 3D-Strukturaufklärung von Makromolekülen (M), dadurch gekenn zeichnet, daß man einen fluoreszenzfähigen Liganden (F) mit einer Fluoreszenzfrequenz im Bereich von (1 bis (2 in das Makromolekul (M) einführt oder dessen räumliche Position zum Makromolekul (M) nach an sich bekannten Verfahren bestimmt, einen oder mehrere photoakitvierbare bifunktionelle Cross-Linker (C) mit einer jeweiligen Anregungsfrequenz im Bereich von (1 bis (2 kovalent zwischen dem nicht-photoaktivierbaren Ende (S3 des Cross-Linkers (C, C', C') und geeigneten funktionellen Gruppen (m) des Makromoleküls (M) unter Lichtausschiuß bindet, das Makromolekül (M) oberhalb des Frequenzintervalls (1 bis (2 mit einer Frequenz (Q bestrahlt, wobei mittels strahlungsioser Ubertragung (Förster-Transfer) auf benachbarte Cross-Linker (C und/oder C') das photoaktivierbare Ende (A und/oder A') des Cross-Linkers (C und/oder C') zur Reaktion mit der Oberfläche des Makromoleküls (M) aktiviert und mit der Oberfläche des Moleküls (M) in Abhähgigkeit des Abstandes des fluoreszenzfähigen Liganden (F) reagiert und die paarweise verknüpften Gruppen durch bioanalytische Verfahren identifiziert.
PCT/EP1999/001008 1998-02-14 1999-02-10 Fluoreszenz-energie-transfer für die aufklärung der 3d-struktur von biomakromolekülen Ceased WO1999041607A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2000531739A JP2002503810A (ja) 1998-02-14 1999-02-10 生体高分子の三次元構造解析のための蛍光エネルギー移動が介在する化学的活性化（ｆｅｔｍａ）
US09/622,161 US6713256B1 (en) 1998-02-14 1999-02-10 Fluorescent energy transfer mediated chemical activation (fetma) for the elucidation of the three-dimensional structure of biomacromolecules
EP99911675A EP1057020A2 (de) 1998-02-14 1999-02-10 Fluoreszenz-energie-transfer für die aufklärung der 3d-struktur von biomakromolekülen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19806169 1998-02-14
DE19806169.2 1998-02-14

Publications (2)

Publication Number Publication Date
WO1999041607A2 WO1999041607A2 (de) 1999-08-19
WO1999041607A3 true WO1999041607A3 (de) 1999-12-09

Family

ID=7857784

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Application Number Title Priority Date Filing Date
PCT/EP1999/001008 Ceased WO1999041607A2 (de) 1998-02-14 1999-02-10 Fluoreszenz-energie-transfer für die aufklärung der 3d-struktur von biomakromolekülen

Country Status (4)

Country Link
US (1) US6713256B1 (de)
EP (1) EP1057020A2 (de)
JP (1) JP2002503810A (de)
WO (1) WO1999041607A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19854196C2 (de) * 1998-11-24 2001-03-15 Xerion Pharmaceuticals Gmbh Verfahren zur Modifikation und Identifikation funktioneller Stellen in Proteinen
DE10036342C2 (de) * 2000-07-26 2002-09-19 Caesar Stiftung Verfahren zur Bestimmung räumlicher Abstände in Polymeren oder Komplexen von Polymeren mit Hilfe von Gemischen von Cross-Linker Molekülen
US7368290B2 (en) * 2003-05-12 2008-05-06 Sandia National Laboratories Structural determination of intact proteins using mass spectrometry

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229943A2 (de) * 1985-12-23 1987-07-29 Molecular Biosystems, Inc. Fluoreszierende stokessche Shift-Sonden für Polynucleotidhybridisationsproben
WO1992014845A1 (en) * 1991-02-26 1992-09-03 Worcester Foundation For Experimental Biology Diagnosing cystic fibrosis and other genetic diseases using fluorescence resonance energy transfer (fret)
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
WO1998048048A2 (en) * 1997-04-21 1998-10-29 Cambridge University Technical Services Ltd. Dna mutation mapping by multiple energy transfer interactions

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5254477A (en) * 1986-06-25 1993-10-19 Trustees Of Tufts College Flourescence intramolecular energy transfer conjugate compositions and detection methods
US6054266A (en) * 1987-12-21 2000-04-25 Applied Biosystems, Inc. Nucleic acid detection with separation
US5252743A (en) * 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5326692B1 (en) * 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
EP0649532B1 (de) * 1992-07-06 1997-11-12 Biomira, Inc. Photoaktivierung von proteinen zu konjugationszwecken
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5612894A (en) * 1995-02-08 1997-03-18 Wertz; David H. System and method for molecular modeling utilizing a sensitivity factor
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US5665966A (en) * 1995-09-29 1997-09-09 Lockheed Martin Idaho Technologies Company Current measuring system
US5900228A (en) * 1996-07-31 1999-05-04 California Institute Of Technology Bifunctional detection agents having a polymer covalently linked to an MRI agent and an optical dye
US6284544B1 (en) * 1997-05-01 2001-09-04 University Of Pennsylvania Determination of metal ions in solution by photoluminescence anisotropy

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229943A2 (de) * 1985-12-23 1987-07-29 Molecular Biosystems, Inc. Fluoreszierende stokessche Shift-Sonden für Polynucleotidhybridisationsproben
WO1992014845A1 (en) * 1991-02-26 1992-09-03 Worcester Foundation For Experimental Biology Diagnosing cystic fibrosis and other genetic diseases using fluorescence resonance energy transfer (fret)
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
WO1998048048A2 (en) * 1997-04-21 1998-10-29 Cambridge University Technical Services Ltd. Dna mutation mapping by multiple energy transfer interactions

Also Published As

Publication number Publication date
US6713256B1 (en) 2004-03-30
JP2002503810A (ja) 2002-02-05
EP1057020A2 (de) 2000-12-06
WO1999041607A2 (de) 1999-08-19

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