WO1999045396A1 - Dispositifs de detection par chimiluminescence - Google Patents

Dispositifs de detection par chimiluminescence Download PDF

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Publication number
WO1999045396A1
WO1999045396A1 PCT/US1999/004785 US9904785W WO9945396A1 WO 1999045396 A1 WO1999045396 A1 WO 1999045396A1 US 9904785 W US9904785 W US 9904785W WO 9945396 A1 WO9945396 A1 WO 9945396A1
Authority
WO
WIPO (PCT)
Prior art keywords
reagent layer
absorbent pad
sample
opening
chemiluminescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/004785
Other languages
English (en)
Inventor
Yongsheng He
Joseph A. Mcclintock
Mohammed A. Chowdhury
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universal Health Watch Inc
Original Assignee
Universal Health Watch Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universal Health Watch Inc filed Critical Universal Health Watch Inc
Priority to AU29837/99A priority Critical patent/AU2983799A/en
Publication of WO1999045396A1 publication Critical patent/WO1999045396A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/528—Atypical element structures, e.g. gloves, rods, tampons, toilet paper
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia

Definitions

  • the present invention relates to diagnostic test devices for rapid detection of analytes using chemiluminescence in a strip format.
  • An advantageous shape is a thin layer lying over the opening although another shape such as a plug may be used, depending on the features of the strip. Fluid preferably moves traverse to the major surface of the reagent layer during transfer to the absorbent pad, although some device configurations may utilize lateral flow along the longest axis of a reagent layer.
  • the reagent layer is a nitrocellulose membrane with antibody bound to it. Preferred in this context is an antibody that reacts with lipopolysaccharide or H7 flagellar antigen from this bacterium.
  • the reagent layer need not be a "layer" per se, but may consist of a pad, rod or other shaped absorbent.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Plasma & Fusion (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Il est possible d'exécuter un essai sensible et rapide en combinant une détection par chimiluminescence avec un tampon à réactif à forte porosité et un tampon absorbant mobile afin d'agir sur le minutage de réaction. Le dispositif d'essai, la technique et les nécessaires correspondant conviennent à des épreuves d'immunodétection axés sur un anticorps ainsi qu'à des épreuves reposant sur des réactions d'hybridation de l'acide nucléique.
PCT/US1999/004785 1998-03-04 1999-03-04 Dispositifs de detection par chimiluminescence Ceased WO1999045396A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU29837/99A AU2983799A (en) 1998-03-04 1999-03-04 Chemiluminescence detection devices

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7670198P 1998-03-04 1998-03-04
US60/076,701 1998-03-04

Publications (1)

Publication Number Publication Date
WO1999045396A1 true WO1999045396A1 (fr) 1999-09-10

Family

ID=22133690

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/004785 Ceased WO1999045396A1 (fr) 1998-03-04 1999-03-04 Dispositifs de detection par chimiluminescence

Country Status (2)

Country Link
AU (1) AU2983799A (fr)
WO (1) WO1999045396A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10058095C2 (de) * 2000-11-03 2003-12-18 Fraunhofer Ges Forschung Vorrichtung zur Bestimmung von Analyten durch Chemilumineszenz
US9482668B2 (en) 2012-02-21 2016-11-01 Laboratory Corporation Of America Holdings Methods and systems for the detection of bacteria
US10519483B2 (en) 2012-02-21 2019-12-31 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
US11591633B2 (en) 2019-09-11 2023-02-28 Laboratory Corporation Of America Holdings Methods and systems for the rapid detection of bacteria using recombinant bacteriophage to express an indicator subunit
US11674124B2 (en) 2019-06-21 2023-06-13 Laboratory Corporation Of America Holdings Methods for producing mutant bacteriophages for the detection of listeria
US11739363B2 (en) 2019-08-26 2023-08-29 Laboratory Corporation Of America Holdings Devices and methods for detecting microorganisms using recombinant reproduction-deficient indicator bacteriophage

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985002466A1 (fr) * 1983-12-02 1985-06-06 Vertrik Bioteknik Ab Dispositif pour analyses chimiques et son utilisation
EP0253371A1 (fr) * 1986-07-15 1988-01-20 Omron Tateisi Electronics Co. Dispositif pour mesurer une substance chimique avec un couvercle facile à manipuler et une seule possibilité de sélection de la fonction
EP0283285A2 (fr) * 1987-03-17 1988-09-21 Diagnostic Systems, Inc. Procédé et appareil pour contrôler des substances analytiques dans des fluides
WO1988007679A1 (fr) * 1987-03-26 1988-10-06 The Secretary Of State For Health In Her Dispositif d'analyse et son utilisation
EP0336454A1 (fr) * 1984-06-01 1989-10-11 Miles Inc. Essai d'hybridation d'un acide nucléique
WO1992002820A1 (fr) * 1990-07-27 1992-02-20 Wisconsin Alumni Research Foundation Anticorps monoclonal specifique contre l'escherichia coli 0157:h7 et 026:h11 entero-hemorragiques et procede de detection

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985002466A1 (fr) * 1983-12-02 1985-06-06 Vertrik Bioteknik Ab Dispositif pour analyses chimiques et son utilisation
EP0336454A1 (fr) * 1984-06-01 1989-10-11 Miles Inc. Essai d'hybridation d'un acide nucléique
EP0253371A1 (fr) * 1986-07-15 1988-01-20 Omron Tateisi Electronics Co. Dispositif pour mesurer une substance chimique avec un couvercle facile à manipuler et une seule possibilité de sélection de la fonction
EP0283285A2 (fr) * 1987-03-17 1988-09-21 Diagnostic Systems, Inc. Procédé et appareil pour contrôler des substances analytiques dans des fluides
WO1988007679A1 (fr) * 1987-03-26 1988-10-06 The Secretary Of State For Health In Her Dispositif d'analyse et son utilisation
WO1992002820A1 (fr) * 1990-07-27 1992-02-20 Wisconsin Alumni Research Foundation Anticorps monoclonal specifique contre l'escherichia coli 0157:h7 et 026:h11 entero-hemorragiques et procede de detection

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10058095C2 (de) * 2000-11-03 2003-12-18 Fraunhofer Ges Forschung Vorrichtung zur Bestimmung von Analyten durch Chemilumineszenz
US9482668B2 (en) 2012-02-21 2016-11-01 Laboratory Corporation Of America Holdings Methods and systems for the detection of bacteria
US10519483B2 (en) 2012-02-21 2019-12-31 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
US12006531B2 (en) 2012-02-21 2024-06-11 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
US11674124B2 (en) 2019-06-21 2023-06-13 Laboratory Corporation Of America Holdings Methods for producing mutant bacteriophages for the detection of listeria
US11739363B2 (en) 2019-08-26 2023-08-29 Laboratory Corporation Of America Holdings Devices and methods for detecting microorganisms using recombinant reproduction-deficient indicator bacteriophage
US12492421B2 (en) 2019-08-26 2025-12-09 Laboratory Corporation Of America Holdings Devices and methods for detecting microorganisms using recombinant reproduction-deficient indicator bacteriophage
US11591633B2 (en) 2019-09-11 2023-02-28 Laboratory Corporation Of America Holdings Methods and systems for the rapid detection of bacteria using recombinant bacteriophage to express an indicator subunit

Also Published As

Publication number Publication date
AU2983799A (en) 1999-09-20

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