WO2000050902A3 - High throughput assay based on the use of a polypeptide binding pair - Google Patents

High throughput assay based on the use of a polypeptide binding pair Download PDF

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Publication number
WO2000050902A3
WO2000050902A3 PCT/GB2000/000669 GB0000669W WO0050902A3 WO 2000050902 A3 WO2000050902 A3 WO 2000050902A3 GB 0000669 W GB0000669 W GB 0000669W WO 0050902 A3 WO0050902 A3 WO 0050902A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
immobilised
high throughput
binding pair
polypeptide binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2000/000669
Other languages
French (fr)
Other versions
WO2000050902A2 (en
Inventor
John Colyer
Roger Kingdon Craig
Antonio Maschio
Mokdad Mezna
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FLUORESCIENCE Ltd
Original Assignee
FLUORESCIENCE Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by FLUORESCIENCE Ltd filed Critical FLUORESCIENCE Ltd
Priority to AU28138/00A priority Critical patent/AU2813800A/en
Priority to EP00906474A priority patent/EP1163526A2/en
Publication of WO2000050902A2 publication Critical patent/WO2000050902A2/en
Publication of WO2000050902A3 publication Critical patent/WO2000050902A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention relates to a method for analysing a sample comprising: immobilising a polypeptide to a physical support; contacting the immobilised polypeptide with a test sample which may contain an agent capable of modifying the immobilised polypeptide; contacting the immobilised polypeptide with a binding partner polypeptide, wherein the binding of this partner polypeptide to the immobilised polypeptide is at least partly dependent on the modification state of the immobilised polypeptide; and measuring the association of the binding partner polypeptide to the immobilised polypeptide.
PCT/GB2000/000669 1999-02-25 2000-02-25 High throughput assay based on the use of a polypeptide binding pair Ceased WO2000050902A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU28138/00A AU2813800A (en) 1999-02-25 2000-02-25 High throughput assay
EP00906474A EP1163526A2 (en) 1999-02-25 2000-02-25 High throughput assay based on the use of a polypeptide binding pair

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9904398.6A GB9904398D0 (en) 1999-02-25 1999-02-25 High throughput assay
GB9904398.6 1999-02-25

Publications (2)

Publication Number Publication Date
WO2000050902A2 WO2000050902A2 (en) 2000-08-31
WO2000050902A3 true WO2000050902A3 (en) 2000-12-14

Family

ID=10848521

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/000669 Ceased WO2000050902A2 (en) 1999-02-25 2000-02-25 High throughput assay based on the use of a polypeptide binding pair

Country Status (4)

Country Link
EP (1) EP1163526A2 (en)
AU (1) AU2813800A (en)
GB (1) GB9904398D0 (en)
WO (1) WO2000050902A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7496132B2 (en) 1999-03-15 2009-02-24 Kg Electronics Inc. Pilot signals for synchronization and/or channel estimation
US7643540B2 (en) 1999-03-15 2010-01-05 Lg Electronics Inc. Pilot signals for synchronization and/or channel estimation
US6791960B1 (en) 1999-03-15 2004-09-14 Lg Information And Communications, Ltd. Pilot signals for synchronization and/or channel estimation
KR100294711B1 (en) 1999-03-15 2001-07-12 서평원 Frame Synchronization Method using Optimal Pilot Symbol
SE0104186D0 (en) * 2001-12-12 2001-12-12 Darcy Birse Method and device

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0392808A2 (en) * 1989-04-10 1990-10-17 The University Of Georgia Research Foundation, Inc. Enzyme luminescence assay
WO1996020211A1 (en) * 1994-12-23 1996-07-04 Ludwig Institute For Cancer Research Phosphotyrosine assay and peptides for use therein
WO1996021040A1 (en) * 1994-12-29 1996-07-11 Research Foundation Of State University Of New York Rapid assay of activators and inhibitors of clotting
WO1996035806A1 (en) * 1995-05-12 1996-11-14 Schering Corporation Surface plasmon resonance based enzymatic assay
WO1998020024A1 (en) * 1996-11-04 1998-05-14 Merck Frosst Canada & Co. Ligands for phosphatase binding assay
WO1998039471A1 (en) * 1997-03-07 1998-09-11 Tropix, Inc. Protease inhibtor assay
WO1999011774A1 (en) * 1997-08-30 1999-03-11 Fluorescience Limited Polypeptides comprising a coiled-coil and an addition site

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0392808A2 (en) * 1989-04-10 1990-10-17 The University Of Georgia Research Foundation, Inc. Enzyme luminescence assay
WO1996020211A1 (en) * 1994-12-23 1996-07-04 Ludwig Institute For Cancer Research Phosphotyrosine assay and peptides for use therein
WO1996021040A1 (en) * 1994-12-29 1996-07-11 Research Foundation Of State University Of New York Rapid assay of activators and inhibitors of clotting
WO1996035806A1 (en) * 1995-05-12 1996-11-14 Schering Corporation Surface plasmon resonance based enzymatic assay
WO1998020024A1 (en) * 1996-11-04 1998-05-14 Merck Frosst Canada & Co. Ligands for phosphatase binding assay
WO1998039471A1 (en) * 1997-03-07 1998-09-11 Tropix, Inc. Protease inhibtor assay
WO1999011774A1 (en) * 1997-08-30 1999-03-11 Fluorescience Limited Polypeptides comprising a coiled-coil and an addition site

Also Published As

Publication number Publication date
AU2813800A (en) 2000-09-14
GB9904398D0 (en) 1999-04-21
WO2000050902A2 (en) 2000-08-31
EP1163526A2 (en) 2001-12-19

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