WO2000053782A2 - Genes cibles d'herbicide et procedes correspondants - Google Patents

Genes cibles d'herbicide et procedes correspondants Download PDF

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Publication number
WO2000053782A2
WO2000053782A2 PCT/EP2000/001884 EP0001884W WO0053782A2 WO 2000053782 A2 WO2000053782 A2 WO 2000053782A2 EP 0001884 W EP0001884 W EP 0001884W WO 0053782 A2 WO0053782 A2 WO 0053782A2
Authority
WO
WIPO (PCT)
Prior art keywords
seq
plant
gene
dna
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2000/001884
Other languages
English (en)
Other versions
WO2000053782A3 (fr
Inventor
David Andrew Patton
Carl Sandidge Ashby
Carla Randall Thomas
John Alan Mcelver
Joshua Zvi Levin
Gregory Joseph Budziszewski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Pharma GmbH Austria
Novartis AG
Syngenta Participations AG
Original Assignee
Novartis Erfindungen Verwaltungs GmbH
Novartis AG
Syngenta Participations AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Erfindungen Verwaltungs GmbH, Novartis AG, Syngenta Participations AG filed Critical Novartis Erfindungen Verwaltungs GmbH
Priority to AU32865/00A priority Critical patent/AU3286500A/en
Priority to EP00910776A priority patent/EP1159434A2/fr
Publication of WO2000053782A2 publication Critical patent/WO2000053782A2/fr
Publication of WO2000053782A3 publication Critical patent/WO2000053782A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Definitions

  • amino acid sequence is substantially similar to SEQ ID NO:2, SEQ ID NO:6, or SEQ ID NO:8, or SEQ ID No:22
  • expression cassettes comprising a promoter operatively linked to a DNA molecule comprising a nucleotide sequence encoding an amino acid sequence substantially similar to SEQ ID NO:2, SEQ ID NO:6, or SEQ ID NO:8, or SEQ ID NO:22
  • a feature of the invention is the identification of a gene in A. thaliana, herein referred to as the 8388 gene, which shows sequence similarity to DEAD box RNA helicase (Luking et al. (1998) Critical Reviews in Biochemistry and Molecular Biology, 33(4): 259- 296).
  • a feature of the invention is the identification of a gene in A. thaliana, herein referred to as the 18048 gene, which shows sequence similarity to ADP-ribosylation factor (Arf) genes (Regad et al. (1993) FEBS Lett. 25: 133-136; Bar-Peled et al.
  • the resulting DNA fragments are cloned into pTRC99a (Pharmacia, Cat no: 27-5007-01 ) for use in bacteria, or into pESC vectors (Stratagene Catalog) for use in yeast; and transformed into a bacterial or yeast strain deficient in 16713 activity by electroporation using the ⁇ iorad Gene Pulser and the manufacturer's conditions.
  • the transformed bacteria or yeast are grown on medium that contains inhibitory concentrations of an inhibitor of 16713 activity and those colonies that grow in the presence of the inhibitor are selected. Colonies that grow in the presence of normally inhibitory concentrations of inhibitor are picked and purified by repeated restreaking. Their plasmids are purified and the DNA sequences of cDNA inserts from plasmids that pass this test are then determined.
  • pTRC99a Pulacia, Cat no: 27-5007-01
  • pESC vectors Stratagene Catalog

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des gènes isolés de Arabidopsis qui codent pour des protéines essentielles au développement normal d'une plante. Elle concerne également des procédés permettant d'utiliser ces protéines pour rechercher de nouveaux herbicides, basés sur le caractère essentiel des gènes dans la croissance et le développement normaux. Elle peut également être mise en application dans une méthode de criblage permettant d'identifier des inhibiteurs qui sont des herbicides potentiels. Elle s'applique également au développement de plantes, de tissus végétaux, de semences et de cellules végétales tolérants aux herbicides.
PCT/EP2000/001884 1999-03-05 2000-03-03 Genes cibles d'herbicide et procedes correspondants Ceased WO2000053782A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU32865/00A AU3286500A (en) 1999-03-05 2000-03-03 Herbicide target genes and methods
EP00910776A EP1159434A2 (fr) 1999-03-05 2000-03-03 Genes cibles d'herbicide et procedes correspondants

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US26338599A 1999-03-05 1999-03-05
US09/263,385 1999-03-05
US30903699A 1999-05-10 1999-05-10
US09/309,036 1999-05-10
US32766299A 1999-06-08 1999-06-08
US09/327,662 1999-06-08
US40819699A 1999-09-29 1999-09-29
US09/408,196 1999-09-29

Publications (2)

Publication Number Publication Date
WO2000053782A2 true WO2000053782A2 (fr) 2000-09-14
WO2000053782A3 WO2000053782A3 (fr) 2000-12-21

Family

ID=27500776

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/001884 Ceased WO2000053782A2 (fr) 1999-03-05 2000-03-03 Genes cibles d'herbicide et procedes correspondants

Country Status (3)

Country Link
EP (1) EP1159434A2 (fr)
AU (1) AU3286500A (fr)
WO (1) WO2000053782A2 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001079514A3 (fr) * 2000-04-18 2002-04-18 Syngenta Participations Ag Genes d' « acide amine » glyoxylate aminotransferase d'origine vegetale et utilisations de ceux-ci
EP1209236A1 (fr) * 2000-11-22 2002-05-29 Bayer Ag Phosphomevalonate kinases d'origine vegetale
EP1316609A1 (fr) * 2001-12-03 2003-06-04 Bayer CropScience AG Acetoacetyl-CoA thiolase et son utilisation pour identifier de nouvelles substances a effet fongicide
WO2002064794A3 (fr) * 2001-01-11 2003-10-02 Syngenta Participations Ag Gene cible d'herbicides et procedes correspondants
EP1407270A4 (fr) * 2001-05-30 2005-05-25 Icoria Inc Procedes pour identifier des inhibiteurs de l'expression ou de l'activite de la 3-oxo-5-alpha-steroide 4-deshydrogenase dans des plantes
EP1434784A4 (fr) * 2001-07-25 2005-06-29 Du Pont Genes impliques dans la biosynthese de l'isopentenyl diphosphate dans le latex de l'hevea brasiliensis
CN118085045A (zh) * 2023-11-29 2024-05-28 玉溪中烟种子有限责任公司 一种促进烟草生长的NtSSP2多肽及其应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120248068B (zh) * 2025-04-25 2025-11-21 长江大学 玉米ZmArf1a基因在玉米品种选育中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4343527A1 (de) * 1993-12-16 1995-06-22 Schering Ag Verfahren zur Identifizierung von Stoffen mit potentieller herbizider oder wachstumsregulatorischer Wirkung mittels pflanzlicher Transporterproteine, Verwendung der Transporterproteine sowie Substanzen mit herbizider und wachstumsregulatorischer Wirkung
US6087563A (en) * 1996-01-29 2000-07-11 Arizona Board Of Regents On Behalf Of The University Of Arizona Cloned arabidopsis p-hydroxyphenyl pyruvic acid dioxygenase DNA

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001079514A3 (fr) * 2000-04-18 2002-04-18 Syngenta Participations Ag Genes d' « acide amine » glyoxylate aminotransferase d'origine vegetale et utilisations de ceux-ci
EP1209236A1 (fr) * 2000-11-22 2002-05-29 Bayer Ag Phosphomevalonate kinases d'origine vegetale
US6790619B2 (en) 2000-11-22 2004-09-14 Bayer Aktiengesellschaft Plant phosphomevalonate kinases
WO2002064794A3 (fr) * 2001-01-11 2003-10-02 Syngenta Participations Ag Gene cible d'herbicides et procedes correspondants
EP1407270A4 (fr) * 2001-05-30 2005-05-25 Icoria Inc Procedes pour identifier des inhibiteurs de l'expression ou de l'activite de la 3-oxo-5-alpha-steroide 4-deshydrogenase dans des plantes
EP1434784A4 (fr) * 2001-07-25 2005-06-29 Du Pont Genes impliques dans la biosynthese de l'isopentenyl diphosphate dans le latex de l'hevea brasiliensis
US6998471B2 (en) 2001-07-25 2006-02-14 E.I. Du Pont De Nemours And Company Polynucleotides encoding an acetyl-CoA acetyltransferase from Hevea brasiliensis, related products, and methods
EP1316609A1 (fr) * 2001-12-03 2003-06-04 Bayer CropScience AG Acetoacetyl-CoA thiolase et son utilisation pour identifier de nouvelles substances a effet fongicide
WO2003048349A3 (fr) * 2001-12-03 2005-03-03 Bayer Cropscience Ag Utilisation d'acetoacetyl-coa thiolase pour l'identification de nouvelles substances presentant une activite fongicide
CN118085045A (zh) * 2023-11-29 2024-05-28 玉溪中烟种子有限责任公司 一种促进烟草生长的NtSSP2多肽及其应用

Also Published As

Publication number Publication date
EP1159434A2 (fr) 2001-12-05
WO2000053782A3 (fr) 2000-12-21
AU3286500A (en) 2000-09-28

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