WO2000077241A3 - Heparanase assay - Google Patents

Heparanase assay Download PDF

Info

Publication number
WO2000077241A3
WO2000077241A3 PCT/GB2000/002117 GB0002117W WO0077241A3 WO 2000077241 A3 WO2000077241 A3 WO 2000077241A3 GB 0002117 W GB0002117 W GB 0002117W WO 0077241 A3 WO0077241 A3 WO 0077241A3
Authority
WO
WIPO (PCT)
Prior art keywords
solid phase
phase support
moiety
substrate
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2000/002117
Other languages
French (fr)
Other versions
WO2000077241A2 (en
Inventor
Paul Ernest Charles Brenchley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CENTRAL MANCHESTER HEALTHCARE NHS TRUST
CENTRAL MANCHESTER HEALTHCARE
Original Assignee
CENTRAL MANCHESTER HEALTHCARE NHS TRUST
CENTRAL MANCHESTER HEALTHCARE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CENTRAL MANCHESTER HEALTHCARE NHS TRUST, CENTRAL MANCHESTER HEALTHCARE filed Critical CENTRAL MANCHESTER HEALTHCARE NHS TRUST
Priority to CA002376534A priority Critical patent/CA2376534A1/en
Priority to AU54125/00A priority patent/AU5412500A/en
Priority to JP2001503682A priority patent/JP2003502054A/en
Priority to EP00938900A priority patent/EP1190250A2/en
Publication of WO2000077241A2 publication Critical patent/WO2000077241A2/en
Publication of WO2000077241A3 publication Critical patent/WO2000077241A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, Konjac gum, Locust bean gum or Guar gum
    • G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method of assaying a sample to determine heparanase activity thereof comprises the steps of (i) incubating the sample in the presence of a first solid phase support having immobilised thereon an HSGAG polymer substrate for the heparanase, the said substrate being insensitive to the action of proteases and the said substrate having bonded thereto a first binding moiety and having further bonded thereto a paracrine cell regulator capable of binding to HSGAG, (ii) treating the incubated sample with a second solid phase support having a second moiety provided thereon capable of immobilising HSGAG polymer substrate cleaved from the first solid phase support on said second solid phase support by binding of said second moiety either with the paracrine cell regulator or with the first binding moiety, (iii) generating a measurable signal the other of the first or second moiety of the cleaved substrate immobilised in the second support solid phase, and (iv) measuring the signal on the second solid phase support separated from the first solid phase support.
PCT/GB2000/002117 1999-06-10 2000-06-12 Heparanase assay Ceased WO2000077241A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002376534A CA2376534A1 (en) 1999-06-10 2000-06-12 Heparanase assay
AU54125/00A AU5412500A (en) 1999-06-10 2000-06-12 Heparanase assay
JP2001503682A JP2003502054A (en) 1999-06-10 2000-06-12 Heparanase assay
EP00938900A EP1190250A2 (en) 1999-06-10 2000-06-12 Heparanase assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9913415.7 1999-06-10
GBGB9913415.7A GB9913415D0 (en) 1999-06-10 1999-06-10 Heparanase assay

Publications (2)

Publication Number Publication Date
WO2000077241A2 WO2000077241A2 (en) 2000-12-21
WO2000077241A3 true WO2000077241A3 (en) 2001-03-22

Family

ID=10855020

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/002117 Ceased WO2000077241A2 (en) 1999-06-10 2000-06-12 Heparanase assay

Country Status (7)

Country Link
EP (1) EP1190250A2 (en)
JP (1) JP2003502054A (en)
AU (1) AU5412500A (en)
CA (1) CA2376534A1 (en)
GB (1) GB9913415D0 (en)
HU (1) HUP0202362A2 (en)
WO (1) WO2000077241A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6656699B2 (en) 2000-09-15 2003-12-02 Reddy Us Therapeutics, Inc. Methods and compositions for glycosidase assays
CH696701A5 (en) * 2001-12-18 2007-10-15 Hoffmann La Roche A method for testing an agent for its ability to inhibit heparanase activity.
FR2843126B1 (en) 2002-08-01 2006-01-27 Cis Bio Int "METHOD OF DETERMINING ENDOGLYCOSIDASE ENZYMATIC ACTIVITY"
KR101578342B1 (en) * 2008-03-31 2015-12-16 가부시키가이샤 시세이도 Preparation for preventing or ameliorating wrinkles, to be taken orally, through injection, or through external application to skin, and cosmetic method
EP2411914A1 (en) 2009-03-24 2012-02-01 Hitachi, Ltd. Storage apparatus and its data control method
KR101736519B1 (en) 2009-09-30 2017-05-16 가부시키가이샤 시세이도 Heparanase-activity inhibitor, wrinkle ameliorating agent containing same, and pharmaceutical composition
ES2687780T3 (en) 2009-09-30 2018-10-29 Shiseido Company, Ltd. Heparanase activity inhibitor
EP2609210B1 (en) * 2010-08-27 2015-06-24 Christian-Albrechts-Universität zu Kiel Method for heparanase activity determination
ES2564426B2 (en) 2014-09-19 2016-09-12 Universidad De Oviedo Marker of eye diseases

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185434A (en) * 1985-02-13 1993-02-09 Sanofi Prolonged-action immunotoxins containing a glycopeptide constituent which inactivates ribosomes, modified on its polysaccharide units
US5332812A (en) * 1986-03-10 1994-07-26 Board Of Regents, The University Of Texas System Solid-phase substrate containing modified heparin
WO1994020512A2 (en) * 1993-03-01 1994-09-15 Glycan Pharmaceuticals, Inc. Analogs for specific oligosaccharide-protein interactions and uses therefor
WO2000003036A1 (en) * 1998-07-10 2000-01-20 Insight Strategy & Marketing Ltd. Method of screening for potential anti-metastatic and anti-inflammatory agents using mammalian heparanase as a probe

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185434A (en) * 1985-02-13 1993-02-09 Sanofi Prolonged-action immunotoxins containing a glycopeptide constituent which inactivates ribosomes, modified on its polysaccharide units
US5332812A (en) * 1986-03-10 1994-07-26 Board Of Regents, The University Of Texas System Solid-phase substrate containing modified heparin
WO1994020512A2 (en) * 1993-03-01 1994-09-15 Glycan Pharmaceuticals, Inc. Analogs for specific oligosaccharide-protein interactions and uses therefor
US5795860A (en) * 1993-03-01 1998-08-18 Repligen Corporation Analogs for specific oligosaccharide-protein interactions and uses therefor
WO2000003036A1 (en) * 1998-07-10 2000-01-20 Insight Strategy & Marketing Ltd. Method of screening for potential anti-metastatic and anti-inflammatory agents using mammalian heparanase as a probe

Also Published As

Publication number Publication date
GB9913415D0 (en) 1999-08-11
CA2376534A1 (en) 2000-12-21
AU5412500A (en) 2001-01-02
HUP0202362A2 (en) 2002-10-28
WO2000077241A2 (en) 2000-12-21
EP1190250A2 (en) 2002-03-27
JP2003502054A (en) 2003-01-21

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