WO2001020005A8 - Regulatorische sequenzen und expressionskassetten für hefen, insbesondere für kluyveromyces - Google Patents

Regulatorische sequenzen und expressionskassetten für hefen, insbesondere für kluyveromyces

Info

Publication number
WO2001020005A8
WO2001020005A8 PCT/EP2000/008662 EP0008662W WO0120005A8 WO 2001020005 A8 WO2001020005 A8 WO 2001020005A8 EP 0008662 W EP0008662 W EP 0008662W WO 0120005 A8 WO0120005 A8 WO 0120005A8
Authority
WO
WIPO (PCT)
Prior art keywords
kluyveromyces
yeasts
regulatory sequences
expression cassettes
dna sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2000/008662
Other languages
English (en)
French (fr)
Other versions
WO2001020005A1 (de
Inventor
Dietmar Becher
Rimantas Siekstele
Danguole Bartkeviciute
Kestutis Sasnauskas
Leopold Doehner
Salah Salim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TAD Pharma GmbH
Original Assignee
TAD Pharma GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by TAD Pharma GmbH filed Critical TAD Pharma GmbH
Priority to AU76505/00A priority Critical patent/AU779818B2/en
Priority to JP2001523776A priority patent/JP2003509054A/ja
Priority to CA002381347A priority patent/CA2381347A1/en
Priority to EP00965925A priority patent/EP1214425A1/de
Priority to US10/070,574 priority patent/US7132522B1/en
Publication of WO2001020005A1 publication Critical patent/WO2001020005A1/de
Publication of WO2001020005A8 publication Critical patent/WO2001020005A8/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Es werden eine DNA-Sequenz, die als Promotor in Hefezellen aktiv ist, ein dieses enthaltendes Expressions- und gegebenenfalls Sekretionssystem, dieses System enthaltende Plasmide, mit der DNA transformierte Wirtszellen sowie Verfahren zur Herstellung von Proteinen und Polypeptiden beschrieben.
PCT/EP2000/008662 1999-09-10 2000-09-05 Regulatorische sequenzen und expressionskassetten für hefen, insbesondere für kluyveromyces Ceased WO2001020005A1 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU76505/00A AU779818B2 (en) 1999-09-10 2000-09-05 Regulatory sequences and expression cassettes for yeasts
JP2001523776A JP2003509054A (ja) 1999-09-10 2000-09-05 酵母用の調節配列および発現カセット
CA002381347A CA2381347A1 (en) 1999-09-10 2000-09-05 Regulatory sequences and expression cassettes for yeasts
EP00965925A EP1214425A1 (de) 1999-09-10 2000-09-05 Regulatorische sequenzen und expressionskassetten für hefen, insbesondere für kluyveromyces
US10/070,574 US7132522B1 (en) 1999-09-10 2000-09-05 Regulatory sequences and expression cassettes for yeasts, especially for kluyveromyces

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19943383.6 1999-09-10
DE19943383A DE19943383B4 (de) 1999-09-10 1999-09-10 Regulatorische Sequenzen und Expressionskassetten für Hefen

Publications (2)

Publication Number Publication Date
WO2001020005A1 WO2001020005A1 (de) 2001-03-22
WO2001020005A8 true WO2001020005A8 (de) 2001-06-07

Family

ID=7921535

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/008662 Ceased WO2001020005A1 (de) 1999-09-10 2000-09-05 Regulatorische sequenzen und expressionskassetten für hefen, insbesondere für kluyveromyces

Country Status (7)

Country Link
US (1) US7132522B1 (de)
EP (1) EP1214425A1 (de)
JP (1) JP2003509054A (de)
AU (1) AU779818B2 (de)
CA (1) CA2381347A1 (de)
DE (1) DE19943383B4 (de)
WO (1) WO2001020005A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5804378B2 (ja) * 2010-02-09 2015-11-04 国立大学法人山口大学 クルイベロマイセス・マルシアヌス由来の高発現プロモーター
US9150835B2 (en) 2011-06-24 2015-10-06 Samsung Electronics Co., Ltd. Modified microorganism for highly efficient production of lactic acid
JP5878396B2 (ja) * 2012-03-02 2016-03-08 トヨタ自動車株式会社 新規プロモーター及びその利用
CN105132452A (zh) * 2015-09-07 2015-12-09 复旦大学 在马克斯克鲁维酵母营养缺陷型菌株中表达融合了组氨酸标签的外源基因的重组载体
CN105063080A (zh) * 2015-09-07 2015-11-18 复旦大学 不含有信号肽的在马克斯克鲁维酵母营养缺陷型菌株中进行外源基因表达的重组载体
CN105063082A (zh) * 2015-09-07 2015-11-18 复旦大学 用于在马克思克鲁维酵母营养缺陷型菌株中进行外源基因分泌表达的重组载体
CN115976094B (zh) * 2022-12-15 2024-02-20 浙江大学杭州国际科创中心 一种提高内源酶分泌的基因工程菌及其构建方法和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4943529A (en) * 1982-05-19 1990-07-24 Gist-Brocades Nv Kluyveromyces as a host strain
JPS59501572A (ja) * 1982-05-19 1984-09-06 ユニリーバー　ナームローゼ　ベンノートシヤープ クルイベロミセス属酵母およびその製造法
AU5811194A (en) * 1992-12-11 1994-07-04 Quest International B.V. The use of the (kluyveromyces marxianus) inulinase gene promoter for protein production

Also Published As

Publication number Publication date
CA2381347A1 (en) 2001-03-22
JP2003509054A (ja) 2003-03-11
AU779818B2 (en) 2005-02-10
AU7650500A (en) 2001-04-17
DE19943383A1 (de) 2001-04-19
WO2001020005A1 (de) 2001-03-22
DE19943383B4 (de) 2006-03-16
US7132522B1 (en) 2006-11-07
EP1214425A1 (de) 2002-06-19

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