WO2001048237A3 - Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens - Google Patents

Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens Download PDF

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Publication number
WO2001048237A3
WO2001048237A3 PCT/DE2000/004610 DE0004610W WO0148237A3 WO 2001048237 A3 WO2001048237 A3 WO 2001048237A3 DE 0004610 W DE0004610 W DE 0004610W WO 0148237 A3 WO0148237 A3 WO 0148237A3
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WIPO (PCT)
Prior art keywords
rna
quickly detecting
microbial dna
kit therefor
detecting microbial
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Ceased
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PCT/DE2000/004610
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English (en)
French (fr)
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WO2001048237A2 (de
WO2001048237A9 (de
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Andreas Hoeft
Frank Stueber
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Priority to EP00991559A priority Critical patent/EP1266028B1/de
Priority to AU33601/01A priority patent/AU3360101A/en
Priority to US10/168,639 priority patent/US7169555B2/en
Priority to DE50014937T priority patent/DE50014937D1/de
Priority to DE10084146T priority patent/DE10084146D2/de
Publication of WO2001048237A2 publication Critical patent/WO2001048237A2/de
Publication of WO2001048237A3 publication Critical patent/WO2001048237A3/de
Anticipated expiration legal-status Critical
Publication of WO2001048237A9 publication Critical patent/WO2001048237A9/de
Priority to US11/592,085 priority patent/US7291724B2/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur schnellen Bestimmung von bakterieller DNS, wobei die Probe mit bakterieller DNS vorgelegt wird, die Probe mittels eines PCR-Verfahrens behandelt und mittels der PCR vervielfachten DNS-Kopien bestimmt wird.
PCT/DE2000/004610 1999-12-23 2000-12-27 Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens Ceased WO2001048237A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP00991559A EP1266028B1 (de) 1999-12-23 2000-12-27 Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens
AU33601/01A AU3360101A (en) 1999-12-23 2000-12-27 Method for quickly detecting microbial dna/rna, kit therefor and the use of saidmethod
US10/168,639 US7169555B2 (en) 1999-12-23 2000-12-27 Method for indentifying microbial DNA or RNA
DE50014937T DE50014937D1 (de) 1999-12-23 2000-12-27 Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens
DE10084146T DE10084146D2 (de) 1999-12-23 2000-12-27 Verfahren zur schnellen Bestimmung von Mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
US11/592,085 US7291724B2 (en) 1999-12-23 2006-11-02 Oligonucleotides for rapidly identifying microbial DNA or RNA

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19962895.5 1999-12-23
DE19962895 1999-12-23
DE10027113.8 2000-05-31
DE10027113A DE10027113A1 (de) 1999-12-23 2000-05-31 Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10168639 A-371-Of-International 2000-12-27
US11/592,085 Continuation US7291724B2 (en) 1999-12-23 2006-11-02 Oligonucleotides for rapidly identifying microbial DNA or RNA

Publications (3)

Publication Number Publication Date
WO2001048237A2 WO2001048237A2 (de) 2001-07-05
WO2001048237A3 true WO2001048237A3 (de) 2002-02-14
WO2001048237A9 WO2001048237A9 (de) 2003-01-23

Family

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Family Applications (1)

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PCT/DE2000/004610 Ceased WO2001048237A2 (de) 1999-12-23 2000-12-27 Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens

Country Status (6)

Country Link
US (2) US7169555B2 (de)
EP (2) EP1266028B1 (de)
AT (2) ATE384803T1 (de)
AU (1) AU3360101A (de)
DE (4) DE10027113A1 (de)
WO (1) WO2001048237A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8323898B2 (en) 2006-02-21 2012-12-04 National University Corporation University Of Toyama Method for identifying pathogenic microorganisms responsible for infection by extracting the DNA of a microorganism

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
CA2501144C (en) 2002-10-23 2015-10-06 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
MXPA05005073A (es) * 2002-11-12 2005-11-17 Becton Dickinson Co Diagnostico de la sepsis o sirs usando perfiles de biomarcadores.
BR0316232A (pt) 2002-11-12 2005-10-04 Becton Dickinson Co Métodos para determinar o estado de sepsia, para prognosticar o começo de sepsia e para diagnosticar a sìndrome de resposta inflamatória sistêmica em um indivìduo
CA2505843A1 (en) 2002-11-12 2004-05-27 Becton, Dickinson And Company Diagnosis of sepsis or sirs using biomarker profiles
EP1426447A1 (de) * 2002-12-06 2004-06-09 Roche Diagnostics GmbH Verfahren zur Detection des pathogenen gram positiven Bacterien, Bvw. Staphylococcus, Enterococcus and Streptococcus
US7718361B2 (en) * 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
JP4377375B2 (ja) 2002-12-06 2009-12-02 エフ．ホフマン−ラ ロシュ アーゲー 病原性生物の多重分析検出
JP2004313181A (ja) 2003-04-02 2004-11-11 Canon Inc 感染症起炎菌検出用プローブ及びプローブセット、ならびに担体及び遺伝子検査方法
WO2005005659A1 (en) * 2003-07-14 2005-01-20 Statens Serum Institut Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp.
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
JP2008538007A (ja) 2005-04-15 2008-10-02 ベクトン，ディッキンソン アンド カンパニー 敗血症の診断
GB0512116D0 (en) * 2005-06-14 2005-07-20 Matforsk Norwegian Food Res In Classification method
US7919242B2 (en) 2005-06-30 2011-04-05 Roche Molecular Systems, Inc. Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
WO2007023461A2 (en) * 2005-08-26 2007-03-01 Philips Intellectual Property & Standards Gmbh Method for detection of micro-organisms and antibiotic resistance markers and nucleic acid oligonucleotides therefor
US8741565B2 (en) * 2005-12-30 2014-06-03 Honeywell International Inc. Oligonucleotide microarray for identification of pathogens
AT504194B1 (de) * 2006-09-07 2008-07-15 Oesterr Rotes Kreuz Bakteriennachweis
FR2908785B1 (fr) * 2006-11-17 2012-09-28 Air Liquide Methodes de detection de microorganismes par pcr
WO2008122002A2 (en) * 2007-04-02 2008-10-09 University Of Utah Research Foundation Organism identification panel
DE102007031137A1 (de) * 2007-06-13 2008-12-18 Attomol Gmbh Molekulare Diagnostika Verfahren und Sonden/Primärsystem zum "real time" Nachweis eines Nukleinsäuretargets
US9090885B2 (en) 2007-07-26 2015-07-28 The University Of Chicago Co-incubating confined microbial communities
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
US20090226900A1 (en) * 2008-03-05 2009-09-10 Helicos Biosciences Corporation Methods for Reducing Contaminants in Nucleic Acid Sequencing by Synthesis
KR101206039B1 (ko) 2008-03-20 2012-11-28 삼성전자주식회사 비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 분리하는 방법,상기 분리된 핵산을 주형으로 하여핵산을 증폭하는 방법 및 상기 고체 지지체를 포함하는핵산 분리 및 증폭 장치
US8669113B2 (en) 2008-04-03 2014-03-11 Becton, Dickinson And Company Advanced detection of sepsis
US20140378337A1 (en) * 2011-12-21 2014-12-25 Wake Forest University Health Sciences Compositions and methods for detecting and identifying bacteria
US20130217024A1 (en) * 2012-02-20 2013-08-22 The Cleveland Clinic Foundation Methods for diagnosing bacteremia and fungemi
EP3286329B1 (de) * 2015-04-23 2019-10-30 PathoFinder B.V. Verfahren für den gleichzeitigen nachweis mehrerer nukleinsäuresequenzen in einer probe
CN105368961A (zh) * 2015-12-15 2016-03-02 天津脉络生物科技有限公司 一种沙雷菌定量检测荧光pcr试剂盒及其应用
CN110832085A (zh) 2017-04-03 2020-02-21 海利克斯拜恩德股份有限公司 用于鉴定微生物感染的方法和装置
WO2018195404A1 (en) * 2017-04-21 2018-10-25 The Board Of The Trustees Of The Leland Stanford Junior University Ultra-sensitive and universal species detection pipeline for next generation sequencing data
US20190062809A1 (en) * 2017-08-24 2019-02-28 Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) Electrochemical detection of bacterial and/or fungal infections
CN109706238B (zh) 2017-10-26 2023-04-07 珠海岐微生物科技有限公司 一种老年性黄斑病变的检测与治疗方法
ES2702117B2 (es) * 2018-08-16 2021-03-04 Univ Madrid Politecnica METODO DE DETECCION POR PCR DE LA BACTERIA Legionella pneumophila EN MUESTRAS AMBIENTALES Y/O CLINICAS
IL283127B1 (en) 2018-11-14 2026-02-01 Smilebiotek Zhuhai Ltd Animal-based models, scanning methods, and methods of treating intraocular diseases or disorders
CN110389230B (zh) * 2019-07-22 2022-06-21 无锡生基医药科技有限公司 一种通用快速dna产品中rna残留定量方法
CN120741108B (zh) * 2025-06-30 2026-02-10 广州盛安医学检验有限公司 一种基于荧光染色技术的支气管肺泡灌洗液快速检测方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5700636A (en) * 1990-10-19 1997-12-23 Becton Dickinson And Company Methods for selectively detecting microorganisms associated with vaginal infections in complex biological samples
NL9301957A (nl) * 1993-11-11 1995-06-01 U Gene Research Bv Werkwijze voor het identificeren van micro-organismen, en daarvoor bruikbare hulpmiddelen.
US5605796A (en) * 1994-07-19 1997-02-25 Behringwerke Ag Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates
WO1997007238A2 (de) 1995-08-17 1997-02-27 Eberhard-Karls-Universität Tübingen Extraktion, amplifikation und sequentielle hybridisierung von pilzzellen-dna sowie verfahren zum nachweisen von pilzzellen in klinischem material
GB9526352D0 (en) * 1995-12-22 1996-02-21 Smithkline Beecham Plc Novel compounds
JP4540754B2 (ja) * 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
EP1185697A2 (de) 1999-04-30 2002-03-13 Qualicon, Inc. Testverfahren, das auf der analyse von dns-schmelzkurven basiert
WO2002014555A2 (en) * 2000-08-11 2002-02-21 University Of Utah Research Foundation Single-labeled oligonucleotide probes

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
AHSEN ET AL.: "Application of a Thermodynamic Nearest-Neighbor Model to Estimate Nucleic Acid Stability and Optimize Probe Design: Prediction of Melting Points of Multiple Mutations of Apolipoprotein B-3500 and Factor V with a Hybridization Probe Genotyping Assay on the LightCycler", CLINICAL CHEMISTRY, vol. 45, no. 12, 1 December 1999 (1999-12-01), pages 2094 - 2101, XP002175788 *
ANTHONY ET AL.: "Rapid Diagnosis of Bacteremia by Universal Amplification of 23S Ribosomal DNA Followed by Hybridization to an Oligonucleotide Array", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 38, no. 2, February 2000 (2000-02-01), pages 781 - 788, XP002176098 *
BERNARD ET AL.: "Homogenous Multiplex Genotyping of Hemochromatosis Mutations with Fluorescent Hybridization Probes", AMERICAN JOURNAL OF PATHOLOGY, vol. 153, no. 4, October 1998 (1998-10-01), pages 1055 - 1061, XP000874040 *
DE SILVA: "Rapid Genotyping and Quantification on the LightCycler with Hybridization Probes", BIOCHEMICA, no. 2, 1998, pages 12 - 15, XP002175789 *
ESPY ET AL.: "Diagnosis of Herpes Simplex Virus Infections in the Clinical Laboratory by LightCycler PCR", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 38, no. 2, February 2000 (2000-02-01), pages 795 - 799, XP002175791 *
KLAUSEGGER ET AL.: "Gram Type-Specific Broad Range PCR Amplification for Rapid Detection of 62 Pathogenic Bacteria", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 37, no. 2, February 1999 (1999-02-01), pages 464 - 466, XP002176097 *
NAUCK ET AL.: "Rapid, Homogenous Genotyping of the 4G/5G Polymorphism in the Promoter Region of the PAI 1 Gene by Fluorescence Resonance Energy Transfer and Probe Melting Curve", CLINICAL CHEMISTRY, vol. 45, no. 8, 1 August 1999 (1999-08-01), pages 1141 - 1147, XP002175787 *
PIETILÄ ET AL.: "Rapid Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi Sensu Stricto by LightCycler Fluorescence Melting Curve Analysis of a PCR Product of the recA Gene", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 38, no. 7, July 2000 (2000-07-01), pages 2756 - 2759, XP002175790 *
RIRIE ET AL.: "Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction", ANALYTICAL BIOCHEMISTRY, 1997, pages 154 - 160, XP001015991 *
SHEPERD ET AL.: "Monitoring of fluorescence during DNA melting as a method for discrimination and detection of PCR products in variety identification", MOLECULAR BREEDING, vol. 4, no. 6, 1998, pages 509 - 517, XP001016033 *
WOO ET AL.: "Identification of Leptospira biflexa by real-time homogenous detection of rapid cycle PCR product", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 35, no. 1, February 1999 (1999-02-01), pages 23 - 30, XP001016145 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8323898B2 (en) 2006-02-21 2012-12-04 National University Corporation University Of Toyama Method for identifying pathogenic microorganisms responsible for infection by extracting the DNA of a microorganism

Also Published As

Publication number Publication date
US7169555B2 (en) 2007-01-30
US7291724B2 (en) 2007-11-06
ATE455870T1 (de) 2010-02-15
US20070042422A1 (en) 2007-02-22
US20050079490A1 (en) 2005-04-14
WO2001048237A2 (de) 2001-07-05
EP1790735A1 (de) 2007-05-30
AU3360101A (en) 2001-07-09
DE50014937D1 (de) 2008-03-13
ATE384803T1 (de) 2008-02-15
EP1266028B1 (de) 2008-01-23
EP1790735B1 (de) 2010-01-20
DE10027113A1 (de) 2001-09-27
DE50015854D1 (de) 2010-03-11
DE10084146D2 (de) 2003-01-16
EP1266028A2 (de) 2002-12-18
WO2001048237A9 (de) 2003-01-23

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