WO2005010209A3 - Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren - Google Patents

Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren Download PDF

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Publication number
WO2005010209A3
WO2005010209A3 PCT/EP2004/008363 EP2004008363W WO2005010209A3 WO 2005010209 A3 WO2005010209 A3 WO 2005010209A3 EP 2004008363 W EP2004008363 W EP 2004008363W WO 2005010209 A3 WO2005010209 A3 WO 2005010209A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
reverse transcription
amplification
pool
derived
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2004/008363
Other languages
English (en)
French (fr)
Other versions
WO2005010209A2 (de
Inventor
Tanja Wille
Christian Korfhage
Eric Lader
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Priority to JP2006520816A priority Critical patent/JP2006528482A/ja
Priority to AU2004259851A priority patent/AU2004259851A1/en
Priority to US10/565,989 priority patent/US20060281092A1/en
Priority to CA002533119A priority patent/CA2533119A1/en
Priority to EP04763506A priority patent/EP1651776A2/de
Publication of WO2005010209A2 publication Critical patent/WO2005010209A2/de
Publication of WO2005010209A3 publication Critical patent/WO2005010209A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur reversen Transkription und/oder Amplifikation eines Produktes aus einer reversen Transkription eines Pools von Nukleinsäuren eines bestimmten Typs, wobei dieser Pool von Nukleinsäuren aus einer komplexen biologischen Probe oder einer enzymatischen Reaktion stammt.
PCT/EP2004/008363 2003-07-24 2004-07-26 Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren Ceased WO2005010209A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2006520816A JP2006528482A (ja) 2003-07-24 2004-07-26 核酸の逆転写及び／または増幅方法
AU2004259851A AU2004259851A1 (en) 2003-07-24 2004-07-26 Method for the reverse transcription and/or amplification of nucleic acids
US10/565,989 US20060281092A1 (en) 2003-07-24 2004-07-26 Method for the reverse transcription and/or amplification of nucleic acids
CA002533119A CA2533119A1 (en) 2003-07-24 2004-07-26 Method for the reverse transcription and/or amplification of nucleic acids
EP04763506A EP1651776A2 (de) 2003-07-24 2004-07-26 Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48964303P 2003-07-24 2003-07-24
US60/489,643 2003-07-24

Publications (2)

Publication Number Publication Date
WO2005010209A2 WO2005010209A2 (de) 2005-02-03
WO2005010209A3 true WO2005010209A3 (de) 2005-05-26

Family

ID=34102910

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/008363 Ceased WO2005010209A2 (de) 2003-07-24 2004-07-26 Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren

Country Status (6)

Country Link
US (1) US20060281092A1 (de)
EP (1) EP1651776A2 (de)
JP (1) JP2006528482A (de)
AU (1) AU2004259851A1 (de)
CA (1) CA2533119A1 (de)
WO (1) WO2005010209A2 (de)

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US7126215B2 (en) 2004-03-30 2006-10-24 Intel Corporation Microelectronic packages including nanocomposite dielectric build-up materials and nanocomposite solder resist
US20060234277A1 (en) * 2005-03-31 2006-10-19 Amgen Inc. Method for selectively blocking hemoglobin RNA amplification
PL1754222T3 (pl) * 2005-04-19 2008-04-30 Dolby Int Ab Uzależniona od energii kwantyzacja służąca do wydajnego kodowania parametrów dźwięku przestrzennego
WO2007050990A2 (en) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers
US10131935B2 (en) * 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids
WO2011032053A1 (en) * 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP3321378B1 (de) 2012-02-27 2021-11-10 Becton, Dickinson and Company Zusammensetzungen für molekularen zählung
EP3301188B1 (de) * 2012-04-16 2020-10-07 Life Technologies Corporation Verfahren zur herstellung von rna-bibliotheken
CN105705659B (zh) 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
CN107406888A (zh) 2015-03-30 2017-11-28 赛卢拉研究公司 用于组合条形编码的方法和组合物
EP3286326B1 (de) 2015-04-23 2025-01-22 Becton, Dickinson and Company Verfahren zur gesamttranskriptomamplifikation
EP3303587A4 (de) * 2015-05-28 2018-11-21 Kaarel Krjutskov Blockierung von oligonukleotiden
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP4435116A3 (de) 2016-08-05 2024-12-18 Bio-Rad Laboratories, Inc. Zweitstrang direkt
IL265478B2 (en) 2016-09-26 2024-10-01 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
CN110506114B (zh) * 2017-06-20 2023-12-15 深圳华大智造科技股份有限公司 Pcr引物对及其应用
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170B1 (de) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molekulare barcodierung auf gegenüberliegenden transkriptenden
SG11202100713VA (en) * 2018-09-25 2021-04-29 Qiagen Sciences Llc Depleting unwanted rna species
EP3861134B1 (de) 2018-10-01 2024-09-04 Becton, Dickinson and Company Bestimmung von 5'-transkriptsequenzen
EP3877520B1 (de) 2018-11-08 2025-03-19 Becton, Dickson And Company Ganze transkriptomanalyse von einzelzellen mittels random-priming
EP3894552B1 (de) * 2018-12-13 2026-01-28 Becton, Dickinson and Company Selektive verlängerung in der vollständigen transkriptomanalyse von einzelzellen
EP3914728B1 (de) 2019-01-23 2023-04-05 Becton, Dickinson and Company Mit antikörpern assoziierte oligonukleotide
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
EP4055160B1 (de) 2019-11-08 2024-04-10 Becton Dickinson and Company Verwendung von random-rpiming zur gewinnung von v(d)j-informationen in voller länge für immunrepertoiresequenzierung
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
CN115279919A (zh) 2020-01-13 2022-11-01 贝克顿迪金森公司 使用含有du的寡核苷酸的细胞捕获
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
WO2025054499A1 (en) * 2023-09-06 2025-03-13 North Carolina State University Compositions and methods relating to enzymatic dna synthesis

Citations (5)

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WO2001032931A2 (en) * 1999-11-02 2001-05-10 Curagen Corporation Method and compositions for selectively inhibiting amplification of sequences in a population of nucleic acid molecules
WO2001060517A2 (de) * 2000-02-15 2001-08-23 Antigene Biotech Gmbh Gefäss zur nukleinsäureanalytik
WO2002000599A1 (de) * 2000-06-27 2002-01-03 Qiagen Gmbh Neue kompositionen für die isolierung und/oder stabilisierung von nukleinsäuren in biologischen materialien
CA2442092A1 (en) * 2001-03-26 2002-10-17 Ribozyme Pharmaceuticals, Inc. Oligonucleotide mediated inhibition of hepatitis b virus and hepatitis c virus replication

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EP1253205A1 (de) * 2001-04-24 2002-10-30 LION Bioscience AG Verfahren zur Blockierung der Amplifizierung von ausgewählten Sequenzen
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
WO2005003370A2 (en) * 2003-06-06 2005-01-13 Gene Logic, Inc. Methods for enhancing gene expression analysis

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US5891625A (en) * 1992-06-05 1999-04-06 Buchardt Ole Use of nucleic acid analogues in the inhibition of nucleic acid amplification
WO2001032931A2 (en) * 1999-11-02 2001-05-10 Curagen Corporation Method and compositions for selectively inhibiting amplification of sequences in a population of nucleic acid molecules
WO2001060517A2 (de) * 2000-02-15 2001-08-23 Antigene Biotech Gmbh Gefäss zur nukleinsäureanalytik
WO2002000599A1 (de) * 2000-06-27 2002-01-03 Qiagen Gmbh Neue kompositionen für die isolierung und/oder stabilisierung von nukleinsäuren in biologischen materialien
CA2442092A1 (en) * 2001-03-26 2002-10-17 Ribozyme Pharmaceuticals, Inc. Oligonucleotide mediated inhibition of hepatitis b virus and hepatitis c virus replication

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Also Published As

Publication number Publication date
US20060281092A1 (en) 2006-12-14
AU2004259851A1 (en) 2005-02-03
JP2006528482A (ja) 2006-12-21
WO2005010209A2 (de) 2005-02-03
CA2533119A1 (en) 2005-02-03
EP1651776A2 (de) 2006-05-03

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