WO2001077335A2 - Genome de listeria monocytogenes, polypeptides et utilisations - Google Patents

Genome de listeria monocytogenes, polypeptides et utilisations

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Publication number
WO2001077335A2
WO2001077335A2 PCT/FR2001/001118 FR0101118W WO0177335A2 WO 2001077335 A2 WO2001077335 A2 WO 2001077335A2 FR 0101118 W FR0101118 W FR 0101118W WO 0177335 A2 WO0177335 A2 WO 0177335A2
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WO
WIPO (PCT)
Prior art keywords
seqld
unknown
similar
polypeptide
listeria monocytogenes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2001/001118
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English (en)
Other versions
WO2001077335A3 (fr
Inventor
Carmen Buchrieser
Lionel Frangeul
Elisabeth Couve
Christophe Rusniok
Hafida Fsihi
Pierre Dehoux
Olivier Dussurget
Farid Chetouani
Hafed Nedjari
Philippe Glaser
Frank Kunst
Pascale Cossart
Justin Daniels
Werner Goebel
Jürgen KREFT
Michael Kuhn
Eva Ng
José Antonio VAZQUEZ-BOLAND
Gustavo Dominguez-Bernal
Patricia Garrido-Garcia
Alberto Tierrez-Martinez
Alexandra Amend
Trinad Chakraborty
Eugen Domann
Torsten Hain
Patrick Berche
Alain Charbit
Lionel Durant
José-Claudio PEREZ-DIAZ
Fernando Baquero
Francisco Garcia Del Portillo
Nuria Gomez-Lopez
Encarna Maduenio
Betriz De Pablos
Jürgen WEHLAND
Uwe KÄRST
Karl-Dieter Entian
Jörg HAUF
Matthias Rose
Hamut Voss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur
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Institut Pasteur
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Publication date
Application filed by Institut Pasteur filed Critical Institut Pasteur
Priority to AU2001254857A priority Critical patent/AU2001254857A1/en
Priority to EP01927973A priority patent/EP1278853A2/fr
Priority to CA002404988A priority patent/CA2404988A1/fr
Priority to JP2001575189A priority patent/JP2004507217A/ja
Publication of WO2001077335A2 publication Critical patent/WO2001077335A2/fr
Publication of WO2001077335A3 publication Critical patent/WO2001077335A3/fr
Anticipated expiration legal-status Critical
Priority to US11/045,004 priority patent/US20060078901A1/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/06—Antiabortive agents; Labour repressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Definitions

  • the subject of the invention is the genomic sequence and nucleotide sequences coding for Listeria monocytogenes polypeptides, such as cell envelope polypeptides, secreted or specific, or involved in metabolism, in the replication process or in virulence, as well as vectors including said sequences and cells or animals transformed by these vectors.
  • the invention also relates to methods for detecting these nucleic acids or polypeptides and to kits for diagnosis of Listeria monocytogenes infection.
  • the invention also relates to a method of selecting compounds capable of modulating bacterial infection and a method of biosynthesis or biodegradation of molecules of interest using said nucleotide sequences or said polypeptides.
  • the invention finally comprises pharmaceutical compositions, in particular vaccine compositions, for the prevention and / or treatment of bacterial infections, in particular by Listeria monocytogenes.
  • Listeria monocytogenes is a facultative intracellular pathogen. It is the causative agent of listeriosis, a food-related infection posing increasing public health problems, with significant economic impact for the European food industry. Listeriosis is the most lethal food-borne infection (approximately 30% mortality). Listeria monocytogenes has the unusual property of being able to cross three barriers: The intestinal barrier, the blood-brain barrier and the placental barrier. The clinical manifestations of listeriosis include meningitis, meningoencephalitis, abortion and septicemia. This infection is opportunistic and mainly affects pregnant women, babies, the elderly and immuno-depressed people, especially people with AIDS.
  • Listeria monocytogenes is also of veterinary importance with a main risk for sheep (sheep) and cattle.
  • Listeria monocytogenes is particularly resistant to stress or extreme conditions and it is important to seek its presence with care not only for food security problems but also for environmental security problems.
  • the present invention therefore relates to a nucleotide sequence of Listeria monocytogenes characterized in that it corresponds to the sequence SEQ ID No. 1.
  • the present invention also relates to a nucleotide sequence of Listeria monocytogenes characterized in that it is chosen from: a) a nucleotide sequence comprising at least 80%, 85%, 90%, 95% or 98% identity with SEQ ID N ° 1; b) a nucleotide sequence hybridizing under conditions of high stringency with SEQ ID No. 1; c) a nucleotide sequence complementary to SEQ ID No. 1 or complementary to a nucleotide sequence as defined in a), or b), or a nucleotide sequence of the corresponding RNA; d) a nucleotide sequence of a fragment representative of SEQ ID No.
  • the present invention also relates to the nucleotide sequences characterized in that they come from SEQ ID No. 1 and in that they code for a polypeptide chosen from polypeptides of sequence SEQ ID No. 2 to SEQ ID No.
  • the present invention also relates more generally to the nucleotide sequences originating from SEQ ID No. 1, and coding for a polypeptide of L. monocytogenes, as they can be isolated from SEQ ID No. 1.
  • nucleotide sequences characterized in that they comprise a nucleotide sequence chosen from: a) a nucleotide sequence coding for a polypeptide chosen from the sequences SEQ ID No. 2 to SEQ ID No. 2854, preferably chosen from the polypeptides from sequence SEQ ID No 2 to SEQ ID No 41 or SEQ ID No 42 to SEQ ID No 64; b) a nucleotide sequence comprising at least 80%, 85%, 90%, 95% or 98% of identity with a nucleotide sequence coding for a polypeptide chosen from the sequences SEQ ID No. 2 to SEQ ID No.
  • 2854 preferably chosen from polypeptides of sequence SEQ ID No 2 to SEQ ID No 41 or SEQ ID No 42 to SEQ ID No 64; c) a nucleotide sequence hybridizing under high stringency conditions with a nucleotide sequence coding for a polypeptide chosen from the sequences SEQ ID No. 2 to SEQ ID No. 2854, preferably chosen from the polypeptides of sequence SEQ ID No.
  • nucleic acid nucleic or nucleic acid sequence, polynucleotide, oligonucleotide, polynucleotide sequence, sequence nucleotide, terms which will be used interchangeably in the present description, is intended to denote a precise sequence of nucleotides, modified or not, making it possible to define a fragment or a region of a nucleic acid, comprising or not unnatural nucleotides, and which may correspond both double stranded DNA, single stranded DNA and transcripts of said DNAs.
  • the nucleic acid sequences according to the invention also include PNA (Peptid Nucleic Acid), or the like.
  • nucleotide sequences in their natural chromosomal environment that is to say in the natural state.
  • sequences which have been isolated and / or purified that is to say that they have been taken directly or indirectly, for example by copying, their environment having been at least partially modified.
  • This also means the nucleic acids obtained by chemical synthesis.
  • percentage of identity between two nucleic acid or amino acid sequences within the meaning of the present invention is meant a percentage of identical nucleotides or amino acid residues between the two sequences to be compared, obtained after the best alignment, this percentage being purely statistical and the differences between the two sequences being distributed randomly and over their entire length.
  • the term “best alignment” or “optimal alignment” is intended to denote the alignment for which the percentage of identity determined as below is the highest. Sequence comparisons between two nucleic acid or amino acid sequences are traditionally carried out by comparing these sequences after having optimally aligned them, said comparison being carried out by segment or by "comparison window" to identify and compare the regions. sequence similarity locale.
  • the optimal alignment of the sequences for the comparison can be carried out, besides manually, by means of the algorithm of local homology of Smith and Waterman (1981, Ad. App. Math. 2: 482), by means of the algorithm of local homology by Neddleman and Wunsch (1970, J. Mol. Biol. 48: 443), using the similarity search method of Pearson and Lipman (1988, Proc. Natl. Acad. Sci. USA 85: 2444 ), using computer software using these algorithms (GAP, BESTFIT, BLAST P, BLAST N, FASTA and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, WI).
  • BLAST program with the BLOSUM 62 matrix. You can also use the PAM or PAM250 matrices.
  • the percentage of identity between two nucleic acid or amino acid sequences is determined by comparing these two optimally aligned sequences in which the nucleic acid or amino acid sequence to be compared may include additions or deletions compared to the reference sequence for optimal alignment between these two sequences.
  • the percentage identity is calculated by determining the number of identical positions for which the nucleotide or the amino acid residue is identical between the two sequences, by dividing this number of identical positions by the total number of positions compared and by multiplying the result obtained by 100 to obtain the percentage of identity between these two sequences.
  • nucleic acid sequences having a percentage identity of at least 80%, preferably 85% or 90%, more preferably 95% or even 98%, after optimal alignment with a reference sequence is meant the nucleic acid sequences having , with respect to the reference nucleic acid sequence, certain modifications such as in particular a deletion, a truncation, an elongation, a chimeric fusion and / or a substitution, in particular punctual, and whose nucleic sequence presents at least 80%, preferably %, 90%, 95% or 98% of identity after optimal alignment with the reference nucleic sequence.
  • They are preferably sequences whose complementary sequences are capable of hybridizing specifically with the reference sequences.
  • the specific hybridization conditions or high stringency will be such that they ensure at least 80%, preferably 85%, 90%, 95% or 98% of identity after optimal alignment between one of the two sequences and the complementary sequence of the other.
  • Hybridization under conditions of high stringency means that the conditions of temperature and ionic strength are chosen in such a way that they allow hybridization to be maintained between two complementary DNA fragments.
  • high stringency conditions of the hybridization step for the purpose of defining the poly nucleotide fragments described above are advantageously as follows.
  • DNA-DNA or DNA-RNA hybridization is carried out in two stages: (1) prehybridization at 42 ° C for 3 hours in phosphate buffer (20 mM, pH 7.5) containing 5 x SSC (1 x SSC corresponds to a 0.15 M NaCl + 0.015 M sodium citrate solution), 50% formamide, 7% sodium dodecyl sulfate (SDS), 10 x Denhardt's, 5% dextran sulfate and 1% salmon sperm DNA; (2) actual hybridization for 20 hours at a temperature depending on the size of the probe (ie: 42 ° C, for a probe of size> 100 nucleotides) followed by 2 washes of 20 minutes at 20 ° C in 2 x SSC + 2% SDS, 1 wash for 20 minutes at 20 ° C in 0.1 x SSC + 0.1% SDS.
  • the last washing is carried out in 0.1 ⁇ SSC + 0.1% SDS for 30 minutes at 60 ° C. for a probe of size> 100 nucleotides.
  • the conditions of high stringency hybridization described above for a polynucleotide of defined size can be adapted by the skilled person for oligonucleotides of larger or smaller size, according to the teaching of Sambrook et al., ( 1989, Molecular cloning: a laboratory manual. 2 nd Ed. Cold Spring Harbor).
  • fragment representative of sequences according to the invention is intended to denote any nucleotide fragment having at least 15 nucleotides, preferably at least 20, 25, 30, 50, 75, 100, 150, 300 and 450 consecutive nucleotides of the sequence from which it originated.
  • representative fragment is meant in particular a nucleic sequence coding for a biologically active fragment of a polypeptide, as defined below.
  • fragment is also meant the intergenic sequences, and in particular the nucleotide sequences carrying the regulatory signals (promoters, terminators, or even enhancers, etc.).
  • ORFs sequences ORFs for "Open Reading Frame"
  • ORFs sequences ORFs for "Open Reading Frame"
  • initiation codon and a stop codon or between two stop codons
  • polypeptides preferably at least 100 amino acids, such as for example, without limitation, the ORFs sequences which will be described later.
  • the numbering of the nucleotide sequences ORFs which will be used subsequently in the present description corresponds to the numbering of the amino acid sequences of the proteins encoded by said ORFs.
  • the representative fragments according to the invention can be obtained for example by specific amplification such as PCR or after digestion with appropriate restriction enzymes of nucleotide sequences according to the invention, this method being described in particular in the work by Sambrook et al. .. Said representative fragments can also be obtained by chemical synthesis when their size is not too large, according to methods well known to those skilled in the art.
  • modified nucleotide sequence any nucleotide sequence obtained by mutagenesis according to techniques well known to those skilled in the art, and comprising modifications, preferably at most 10% of modified nucleotides, relative to the normal sequences, for example mutations in the regulatory and / or promoter sequences for the expression of the polypeptide, in particular leading to a modification of the level of expression or of the activity of said polypeptide.
  • modified nucleotide sequence is also meant any nucleotide sequence coding for a modified polypeptide as defined below.
  • the representative fragments according to the invention can also be probes or primers, which can be used in methods of detection, identification, assay or amplification of nucleic sequences.
  • a probe or primer is defined, within the meaning of the invention, as being a fragment of single-stranded nucleic acids or a denatured double-stranded fragment comprising for example from 12 bases to a few kb, in particular from 15 to a few hundred bases, preferably from 15 to 50 or 100 bases, and having a specificity of hybridization under determined conditions to form a hybridization complex with a target nucleic acid.
  • the probes and primers according to the invention can be labeled directly or indirectly with a radioactive or non-radioactive compound by methods well known to those skilled in the art, in order to obtain a detectable and / or quantifiable signal.
  • the unlabeled polynucleotide sequences according to the invention can be used directly as a probe or primer.
  • sequences are generally marked to obtain sequences which can be used for numerous applications.
  • the labeling of the primers or probes according to the invention is carried out with radioactive elements or with non-radioactive molecules.
  • Non-radioactive entities are selected from ligands such as biotin, avidin, streptavidin, dioxygenin, haptens , dyes, luminescent agents such as radioluminescent, chemoluminescent, bioluminescent, fluorescent, phosphorescent agents.
  • the polynucleotides according to the invention can thus be used as a primer and / or probe in methods using in particular the PCR technique (polymerase chain reaction) (Rolfs et al., 1991, Berlin: Springer-Verlag).
  • This technique requires the choice of pairs of oligonucleotide primers framing the fragment which must be amplified.
  • the amplified fragments can be identified, for example after an electrophoresis in agarose or polyacrylamide gel, or after a chromatographic technique such as gel filtration or ion exchange chromatography, and then sequenced.
  • the specificity of the amplification can be controlled by using, as primer, the nucleotide sequences of polynucleotides of the invention as template, plasmids containing these sequences or else the derived amplification products.
  • the amplified nucleotide fragments can be used as reagents in hybridization reactions in order to demonstrate the presence, in a biological sample, of a target nucleic acid of sequence complementary to that of said amplified nucleotide fragments.
  • the invention also relates to the nucleic acids capable of being obtained by amplification using primers according to the invention.
  • Other techniques for amplifying the target nucleic acid can advantageously be used as an alternative to PCR (PCR-like) using pairs of primers of nucleotide sequences according to the invention.
  • PCR-like is meant to denote all the methods implementing direct or indirect reproductions of the nucleic acid sequences, or in which the labeling systems have been amplified, these techniques are of course known, in general these are amplification of DNA with a polymerase; when the original sample is an RNA, a reverse transcription should be carried out beforehand.
  • the target polynucleotide to be detected is an mRNA
  • an enzyme of reverse transcriptase type in order to obtain a cDNA from the mRNA contained in the biological sample.
  • the cDNA obtained will then serve as a target for the primers or probes used in the amplification or detection method according to the invention.
  • the probe hybridization technique can be performed in various ways (Matthews et al., 1988, Anal. Biochem., 169, 1-25).
  • the most general method consists in immobilizing the nucleic acid extracted from cells of different tissues or of cells in culture on a support (such as nitrocellulose, nylon, polystyrene) and in incubating, under well defined conditions, the target nucleic acid immobilized with the probe. After hybridization, the excess probe is eliminated and the hybrid molecules formed are detected by the appropriate method (measurement of radioactivity, fluorescence or enzymatic activity linked to the probe).
  • the latter can be used as capture probes.
  • a probe called a “capture probe”
  • a probe is immobilized on a support and is used to capture by specific hybridization the target nucleic acid obtained from the biological sample to be tested and the target nucleic acid is then detected.
  • a second probe called a “detection probe”, marked by an easily detectable element.
  • the antisense oligonucleotides that is to say those whose structure ensures, by hybridization with the target sequence, an inhibition of the expression of the corresponding product. Mention should also be made of sense oligonucleotides which, by interaction with proteins involved in the regulation of the expression of the corresponding product, will induce either an inhibition or an activation of this expression.
  • the probes or primers according to the invention are immobilized on a support, covalently or non-covalently.
  • the support can be a DNA chip or a high density filter, also objects of the present invention.
  • DNA chip or high density filter is intended to denote a support on which DNA sequences are fixed, each of which can be identified by its geographic location. These chips or filters differ mainly in their size, the material of the support, and possibly the number of DNA sequences attached to them.
  • the probes or primers according to the first invention can be fixed on solid supports, in particular DNA chips, by various manufacturing methods.
  • a synthesis can be carried out in situ by photochemical addressing or by ink jet.
  • Other techniques consist in carrying out an ex situ synthesis and in fixing the probes on the support of the DNA chip by addressing. mechanical, electronic or inkjet.
  • a nucleotide sequence (probe or primer) according to the invention therefore allows the detection and / or amplification of specific nucleic sequences.
  • the detection of these said sequences is facilitated when the probe is fixed to a DNA chip, or to a high density filter.
  • DNA chips or high density filters makes it possible to determine the expression of genes in an organism having a genomic sequence close to L. monocytogenes EGD-e.
  • the genomic sequence of L. monocytogenes EGD-e supplemented by the identification of all the genes of this organism, as presented in the present invention, serves as the basis for the construction of these DNA chips or filter.
  • the preparation of these filters or chips consists in synthesizing oligonucleotides, corresponding to the 5 'and 3' ends of the genes. These oligonucleotides are chosen using the genomic sequence and its annotations disclosed by the present invention. The pairing temperature of these oligonucleotides at the corresponding places on the DNA should be approximately the same for each oligonucleotide. This makes it possible to prepare DNA fragments corresponding to each gene by the use of appropriate PCR conditions in a highly automated environment. The amplified fragments are then immobilized on filters or supports in glass, silicon or synthetic polymers and these media are used for hybridization.
  • filters and / or chips and of the corresponding annotated genomic sequence makes it possible to study the expression of large sets, or even of all of the genes in the microorganisms associated with Listeria monocytogenes, by preparing the complementary DNAs, and by hybridizing them to DNA or to oligonucleotides immobilized on filters or chips. Also, the filters and / or the chips make it possible to study the variability of the strains or of the species, by preparing the DNA of these organisms and by hybridizing them to the DNA or to the oligonucleotides immobilized on the filters or the chips.
  • the differences between the genomic sequences of the different strains or species can greatly affect the intensity of the hybridization and therefore disturb the interpretation of the results. It may therefore be necessary to have the precise sequence of the genes of the strain that we wish to study.
  • the method of detecting genes described later in detail involving determining the sequence of random fragments of a genome, and organizing them according to the complete genome sequence of Listeria monocytogenes EGD-e disclosed in the present invention, can be very helpful.
  • the nucleotide sequences according to the invention can be used in DNA chips to carry out the analysis of mutations. This analysis is based on the constitution of chips capable of analyzing each base of a nucleotide sequence according to the invention. In particular, it will be possible to implement micro-sequencing techniques on a DNA chip.
  • the mutations are detected by extension of immobilized primers hybridizing to the matrix of the sequences analyzed, just in position adjacent to that of the mutated nucleotide sought.
  • a single-stranded matrix, RNA or DNA, of the sequences to be analyzed will advantageously be prepared according to conventional methods, from products amplified according to PCR type techniques.
  • the single-stranded DNA or RNA matrices thus obtained are then deposited on the DNA chip, under conditions allowing their specific hybridization to the immobilized primers.
  • a thermostable polymerase for example Tth or Taq DNA polymerase, specifically extends the 3 'end of the immobilized primer with a labeled nucleotide analog complementary to the nucleotide at the variable site position; for example a thermal cycle is carried out in the presence of fluorescent dideoxyribonucleotides.
  • the experimental conditions will be adapted in particular to the chips used, to the immobilized primers, to the polymerases used, and to the chosen labeling system.
  • microsequencing compared to techniques based on probe hybridization, is that it makes it possible to identify all the variable nucleotides with optimal discrimination under homogeneous reaction conditions; used on DNA chips, it allows optimal resolution and specificity for routine and industrial detection of mutations in multiplex.
  • the use of high density filters and / or chips thus makes it possible to obtain new knowledge on the regulation of genes in organisms of industrial importance, and in particular the listeria propagated under various conditions. It also allows rapid identification of the differences between the genomes of the strains used in multiple industrial applications.
  • a DNA chip or filter can be an extremely useful tool for the determination, detection and / or identification of a microorganism.
  • the DNA chips according to the invention are also preferred, which also contain at least one nucleotide sequence of a microorganism other from Listeria monocytogenes, immobilized on the support of said chip.
  • the microorganism chosen is from bacteria of the genus Listeria (hereinafter designated as bacteria associated with L. monocytogenes), or variants of Listeria monocytogenes EGD-e.
  • a DNA chip or a filter according to the invention is a very useful element in certain kits or necessary for the detection and / or identification of microorganisms, in particular bacteria belonging to the species Listeria monocytogenes or associated microorganisms, also objects of the invention.
  • DNA chips or filters according to the invention containing probes or primers specific to Listeria monocytogenes, are very advantageous elements of kits or necessary for the detection and / or quantification of the expression of Listeria genes monocytogenes (or associated microorganisms).
  • the control of gene expression is a critical point for optimizing the growth and yield of a strain, either by allowing the expression of one or more new genes, or by modifying the expression of genes already present in the cell.
  • the present invention provides all the naturally active sequences in L. monocytogenes allowing gene expression. It thus allows the determination of all the sequences expressed in L. monocytogenes. It also provides a tool for identifying genes whose expression follows a given pattern. To achieve this, the DNA of all or part of the genes of L. monocytogenes can be amplified using primers according to the invention, then fixed to a support such as for example glass or nylon or a DNA chip, in order to build a tool to monitor the expression profile of these genes.
  • This tool consisting of this support containing the coding sequences, serves as a hybridization matrix for a mixture of labeled molecules reflecting the messenger RNAs expressed in the cell (in particular the labeled probes according to the invention).
  • each control sequence present upstream of the segments serving as probes and to monitor their activity using an appropriate means such as a reporter gene (luciferase, ⁇ -galactosidase, GFP for " Green Fluorescent Protein ”).
  • a reporter gene luciferase, ⁇ -galactosidase, GFP for " Green Fluorescent Protein ”.
  • These isolated sequences can then be modified and assembled by metabolic engineering with sequences of interest with a view to their optimal expression. Thanks to the genomic sequence presented in the present invention, those skilled in the art will be able to identify the genes coding for proteins regulating the transcription of the genes of L. monocytogenes.
  • Table I provides the list of open reading frames (ORF for “Open Reading Frame) identified on the genome of Listeria monocytogenes (SEQ ID No. 1), with their position on said genome, and the putative functions which can be assigned to them.
  • ORF Open Reading Frame
  • Altering the structure or integrity of these genes may allow the expression of target genes controlled by target promoters of these regulators to be modified.
  • target genes controlled by target promoters of these regulators may be modified.
  • those skilled in the art will be able to choose the regulator or regulators relevant to the desired application as well as their target, which allows the optimization of the expression of genes of interest.
  • the use of the tools described above, such as DNA chips also makes it possible to identify all of the genes whose regulation is modified by the inactivation of certain genes. It is thus possible to select a set of control sequence responding, except for nuances, to the same type of regulation. These sequences can then be used to control the expression of genes of interest.
  • the invention also relates to the polypeptides coded by a nucleotide sequence according to the invention, preferably, by a fragment representative of the sequence SEQ ID No. 1 and corresponding to an ORF sequence, as described in Table L
  • the Listeria monocytogenes polypeptides characterized in that they are chosen from polypeptides of sequence SEQ ID No. 2 to SEQ ID No. 2854, preferably of sequence SEQ ID No. 2 to SEQ ID No. 41 and SEQ ID No. 42 to SEQ ID No. 64 are subject of the invention.
  • the invention also includes the polypeptides characterized in that they comprise a polypeptide chosen from: a) a polypeptide according to the invention; b) a polypeptide having at least 80%, preferably 85%, 90%, 95% and 98% identity with a polypeptide according to the invention; c) a fragment of at least 5 amino acids of a polypeptide according to the invention, or as defined in b); d) a biologically active fragment of a polypeptide according to the invention, or as defined in b) or c); and e) a modified polypeptide of a polypeptide according to the invention, or as defined in b), c) or d).
  • the nucleotide sequences coding for the polypeptides described above are also subject of the invention.
  • polypeptides polypeptide sequences, peptides and proteins are interchangeable.
  • polypeptides in natural form that is to say that they are not taken in their natural environment but that they could have been isolated or obtained by purification from natural sources, or obtained by genetic recombination, or by chemical synthesis, and that they can then contain non-natural amino acids as will be described later.
  • polypeptide having a certain percentage of identity with another which will also be designated by homologous polypeptide, is intended to denote the polypeptides having, with respect to the natural polypeptides, certain modifications, in particular a deletion, addition or substitution of at least an amino acid, truncation, elongation, chimeric solution and / or mutation, or polypeptides with post-translational modifications.
  • homologous polypeptides those whose amino acid sequence have at least 80%, preferably 85%, 90%, 95% and 98% of homology with the amino acid sequences of the polypeptides according to the invention are preferred. .
  • amino acids In the case of a substitution, one or more consecutive amino acid (s)) or not consecutive are replaced by “equivalent” amino acids.
  • the expression “equivalent amino acids” aims here to designate any amino acid capable of being substituted for one of the amino acids of the basic structure without however essentially modifying the biological activities of the corresponding peptides and as they will be defined by the following.
  • amino acids can be determined either on the basis of their structural homology with the amino acids for which they are substituted, or on results of comparative tests of biological activity between the various polypeptides capable of being carried out.
  • Leucine can thus be replaced by valine or isoleucine, aspartic acid by glutamine acid, glutamine by asparagine, arginine by lysine, etc. reverse substitutions being naturally possible in the same conditions.
  • homologous polypeptides also correspond to the polypeptides encoded by the homologous or identical nucleotide sequences, as defined above and thus include, in the present definition, polypeptides which are mutated or correspond to inter or intra species variations, which may exist in Listeria, and which correspond in particular to truncations, substitutions, deletions and / or additions, of at least one amino acid residue.
  • the percentage of identity between two polypeptides is calculated in the same way as between two nucleic acid sequences.
  • the percentage of identity between two polypeptides is calculated after optimal alignment of these two sequences, over a window of maximum homology.
  • the same algorithms can be used as for the nucleic acid sequences.
  • biologically active fragment of a polypeptide according to the invention is intended to denote in particular a fragment of polypeptide, as defined below, having at least one of the biological characteristics of the polypeptides according to the invention, in particular in that it is able to exercise in general even partial activity, such as for example: - an enzymatic (metabolic) activity or an activity which may be involved in the biosynthesis or biodegradation of organic or inorganic compounds;
  • fragment of polypeptides according to the invention is intended to denote a polypeptide comprising at least 5 amino acids, preferably 10, 15, 25, 50, 100 and 150 amino acids.
  • Polypeptide fragments can also be prepared by chemical synthesis, from hosts transformed by an expression vector according to the invention which contain a nucleic acid allowing the expression of said fragment, and placed under the control of regulatory elements and / or appropriate expression.
  • modified polypeptide of a polypeptide according to the invention is meant a polypeptide obtained by genetic recombination or by chemical synthesis as described below, which exhibits at least one modification with respect to the normal sequence, preferably at most 10 % of amino acids modified compared to the normal sequence. These modifications can be carried in particular on amino acids necessary for the specificity or the efficiency of the activity, or at the origin of the structural conformation, of the charge, or of the hydrophobicity of the polypeptide according to the invention. It is thus possible to create polypeptides of equivalent, increased or decreased activity, or of equivalent specificity, narrower or wider.
  • modified polypeptides mention should be made polypeptides in which up to five amino acids can be modified, truncated at the N or C-terminus, or deleted, or added.
  • modifications of a polypeptide have in particular the aim of: allowing its implementation in processes of biosynthesis or biodegradation of organic or inorganic compounds,
  • Chemical synthesis also has the advantage of being able to use unnatural amino acids or non-peptide bonds. Thus, it may be advantageous to use unnatural amino acids, for example in D form, or analogs of amino acids, in particular suffering forms.
  • the present invention provides the nucleotide sequence of the genome of Listeria monocytogenes EGD-e, as well as certain polypeptide sequences. Those skilled in the art will be able to determine the other ORFs, using known methods, and appropriate software.
  • the present invention also relates to a process for the production of vitamin B12, characterized in that the starting substrate is supplied to a host cell containing the genes corresponding to SEQ ID No. 42 to SEQ ID No. 64 , that it is cultivated under conditions suitable for the production of vitamin B12, and that said vitamin is recovered.
  • the host cell is a bacterial cell, more preferably, a bacterium of the genus Bacillus or Listeria.
  • a process for the production of vitamin B12 using a nucleic sequence, or polypeptide according to the invention, or a host cell according to the invention or an animal or a plant according to the invention is also an object of the present invention.
  • / corresponding coding nucleic acid ⁇ may be determined by a person skilled in the art at
  • the invention relates to a nucleotide sequence according to the invention characterized in that it codes for a polypeptide
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for an envelope polypeptide
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in the biosynthesis of amino acids.
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in the biosynthesis of cofactors, prosthetic groups and transporters.
  • the invention relates to a nucleotide sequence according to the invention characterized in that it codes for a polypeptide of Listeria monocytogenes or one of its fragments involved in the cellular machinery.
  • the invention relates to a nucleotide sequence according to the invention characterized in that it codes for a polypeptide of Listeria monocytogenes or one of its fragments involved in the central intermediate metabolism.
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in energy metabolism.
  • the invention relates to a nucleotide sequence according to the invention characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in the metabolism of fatty acids and phospholipids.
  • the invention relates to a nucleotide sequence according to the invention characterized in that it codes for a polypeptide of Listeria monocytogenes or one of its fragments involved in the metabolism of nucleotides, purines, pyrimidines or nucleosides.
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in regulatory functions.
  • the invention relates to a nucleotide sequence according to the invention characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in the replication process.
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in the transcription process.
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in the translation process.
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in the protein transport and binding process.
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in adaptation to atypical conditions.
  • the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria polypeptide monocytogenes or a fragment thereof in sensitivity to drugs and the like.
  • the invention relates to a nucleotide sequence according to the invention characterized in that it codes for a Listeria monocytogenes polypeptide or one of its fragments involved in the functions relating to transposons.
  • the invention relates to a nucleotide sequence according to the invention characterized in that it codes for a specific polypeptide of
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of
  • the subject of the invention is a polypeptide according to the invention, characterized in that that it is a cell envelope or surface polypeptide of Listeria monocytogenes or one of its fragments.
  • it is a polypeptide of sequence SEQ ID No. 2 to
  • the subject of the invention is a polypeptide according to the invention, characterized in that it is a polypeptide of
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in cellular machinery.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in central intermediate metabolism.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in energy metabolism.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in the metabolism of fatty acids and phospholipids.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in the metabolism of nucleotides, purines, pyrimidines or nucleosides.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in regulatory functions.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in the replication process.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in the transcription process.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in the translation process.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in the protein transport and binding process.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in the adaptation to the conditions atypical.
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments in the sensitivity to drugs and the like .
  • the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria monocytogenes or one of its fragments involved in the functions relating to transposons .
  • the invention relates to a polypeptide according to the invention, characterized in that it is a specific polypeptide of Listeria monocytogenes or one of its fragments.
  • a subject of the present invention is also the nucleotide and / or polypeptide sequences according to the invention, characterized in that said sequences are recorded on a recording medium the shape and nature of which facilitate reading, analysis and / or the exploitation of said sequence (s).
  • These supports can also contain other information extracted from the present invention, in particular analogies with already known sequences, and / or information concerning the nucleotide sequences and / or polypeptides of other microorganisms in order to facilitate the comparative analysis and the exploitation of the results obtained.
  • recording media particular preference is given to media readable by a computer, such as magnetic, optical, electric or hybrid, in particular computer diskettes, CD-ROMs, computer servers. Such recording media are also subject of the invention.
  • the recording media according to the invention are very useful for the choice of primers or nucleotide probes for the determination of genes in Listeria monocytogenes or strains close to this organism.
  • the use of these supports for studying the genetic polymorphism of strains close to Listeria monocytogenes, in particular by determining the regions of collinearity is very useful insofar as these supports provide not only the nucleotide sequence of the genome of Listeria monocytogenes egb, but also the genomic organization in said sequence.
  • the uses of recording media according to the invention are also objects of the invention.
  • sequence comparison software such as the Blast software, or the software of the GCG kit, described above.
  • the invention also relates to the cloning and / or expression vectors, which contain a nucleotide sequence according to the invention.
  • a nucleotide sequence encoding cell envelope or surface polypeptides, or involved in cellular machinery, in particular secretion, central intermediate metabolism, in particular sugar production, energy metabolism, processes synthesis of vitamin B12, transcription and translation, synthesis of polypeptides.
  • the vectors according to the invention preferably comprise elements which allow the expression and / or the secretion of the nucleotide sequences in a determined host cell.
  • the vector must then include a promoter, translation initiation and termination signals, as well as suitable regions for transcription regulation. It must be able to be maintained stably in the host cell and may possibly have specific signals which specify the secretion of the translated protein. These various elements are chosen and optimized by a person skilled in the art according to the cell host used. To this end, the nucleotide sequences according to the invention can be inserted into vectors with autonomous replication within the chosen host, or be integrative vectors of the chosen host.
  • Such vectors are prepared by methods commonly used by those skilled in the art, and the resulting clones can be introduced into an appropriate host by standard methods, such as lipofection, electroporation, heat shock, or chemical methods .
  • the vectors according to the invention are for example vectors of plasmid or viral origin. They are useful for transforming host cells in order to clone or express the nucleotide sequences according to the invention.
  • the invention also includes host cells transformed with a vector according to the invention.
  • the cell host can be chosen from prokaryotic or eukaryotic systems, for example bacterial cells but also yeast cells or animal cells, in particular mammalian cells. You can also use insect cells or plant cells.
  • the preferred host cells according to the invention are in particular prokaryotic cells, preferably bacteria belonging to the genus Listeria, to the species Listeria monocytogenes, or the microorganisms associated with the species Listeria monocytogenes.
  • the invention also relates to animals, except humans, which comprise a cell transformed according to the invention.
  • the cells transformed according to the invention can be used in processes for the preparation of recombinant polypeptides according to the invention.
  • the methods for preparing a polypeptide according to the invention in recombinant form characterized in that they use a vector and / or a cell transformed with a vector according to the invention are themselves included in the present invention.
  • a cell transformed with a vector according to the invention is cultivated under conditions which allow the expression of said polypeptide and said recombinant peptide is recovered.
  • the host cells according to the invention can also be used for the preparation of food compositions, which are themselves subject of the present invention.
  • the cell host can be chosen from prokaryotic or eukaryotic systems.
  • a vector according to the invention carrying such a sequence can therefore be advantageously used for the production of recombinant proteins, intended to be secreted. Indeed, the purification of these recombinant proteins of interest will be facilitated by the fact that they are present in the supernatant of the cell culture rather than inside the host cells.
  • the polypeptides according to the invention can also be prepared by chemical synthesis. Such a preparation process is also an object of the invention.
  • a person skilled in the art knows the chemical synthesis processes, for example the techniques implementing solid phases (see in particular Steward et al., 1984, Solid phase peptides synthesis, Pierce Chem. Company, Rockford, 111, 2nd ed., (1984)) or techniques using partial solid phases, by condensation of fragments or by synthesis in conventional solution.
  • the polypeptides obtained by chemical synthesis and which may contain corresponding unnatural amino acids are also included in the invention.
  • the invention further relates to hybrid polypeptides having at least one polypeptide or a fragment thereof according to the invention, and a sequence of a polypeptide capable of inducing an immune response in humans or animals.
  • the antigenic determinant is such that it is capable of inducing a humoral and / or cellular response.
  • Such a determinant may comprise a polypeptide or one of its fragments according to the invention in glycosylated form used with a view to obtaining immunogenic compositions capable of inducing the synthesis of antibodies directed against multiple epitopes.
  • Said polypeptides or their glycosylated fragments also form part of the invention.
  • hybrid molecules can consist in part of a molecule carrying polypeptides or their fragments according to the invention, associated with a possibly immunogenic part, in particular an epitope of diphtheria toxin, tetanus toxin, a surface antigen of the virus.
  • hepatitis B (patent FR 79 21811), the VP1 antigen of the polio virus or any other toxin or viral or bacterial antigen.
  • Hybrid molecule synthesis methods include the methods used in genetic engineering to construct hybrid nucleotide sequences coding for the polypeptide sequences sought. We can, for example, advantageously refer to the technique for obtaining genes coding for fusion proteins described by Minton in 1984.
  • the invention also includes the vectors characterized in that they contain one of said hybrid nucleotide sequences.
  • the host cells transformed by said vectors, the transgenic animals comprising one of said transformed cells as well as the methods for preparing recombinant polypeptides using said vectors, said transformed cells and / or said transgenic animals are of course also part of the invention.
  • the coupling between a polypeptide according to the invention and an immunogenic polypeptide can be carried out chemically, or biologically.
  • one or more binding element (s), in particular amino acids to facilitate the coupling reactions between the polypeptide according to the invention, and the immunostimulatory polypeptide
  • the covalent coupling of the immunostimulatory antigen can be produced at the N or C-terminal end of the polypeptide according to the invention.
  • the bifunctional reagents allowing this coupling are determined as a function of the end chosen to achieve this coupling, and the coupling techniques are well known to those skilled in the art.
  • the conjugates resulting from a coupling of peptides can also be prepared by genetic recombination.
  • the hybrid (conjugated) peptide can in fact be produced by recombinant DNA techniques, by insertion or addition to the DNA sequence coding for the polypeptide according to the invention, of a sequence coding for the peptide (s) ) antigen (s), immunogen (s) or hapten (s). These techniques for preparing hybrid peptides by genetic recombination are well known to those skilled in the art (see for example Makrides, 1996, Microbiological Reviews 60, 512-538).
  • said immune polypeptide is chosen from the group of peptides containing toxoids, in particular diphtheria toxoid or tetanus toxoid, proteins derived from Streptococcus (such as the protein binding to human serum albumin), OMPA membrane proteins and protein complexes of outer membranes, vesicles of outer membranes or heat shock proteins.
  • toxoids diphtheria toxoid or tetanus toxoid
  • proteins derived from Streptococcus such as the protein binding to human serum albumin
  • OMPA membrane proteins protein complexes of outer membranes, vesicles of outer membranes or heat shock proteins.
  • hybrid polypeptides according to the invention are very useful for obtaining monoclonal or polyclonal antibodies capable of specifically recognizing the polypeptides according to the invention. Indeed, a hybrid polypeptide according to the invention allows the potentiation of the immune response, against the polypeptide according to the invention coupled to the immunogenic molecule. Such monoclonal or polyclonal antibodies, their fragments, or chimeric antibodies, recognizing the polypeptides according to the invention, are also objects of the invention.
  • the specific monoclonal antibodies can be obtained according to the conventional hybridoma culture method described by Kôhler and Mlstein (1975, Nature 256, 495).
  • the antibodies according to the invention are, for example, chimeric antibodies, humanized antibodies, Fab fragments, or F (ab ') 2 . It can also be in the form of an immunoconjugate or of labeled antibodies in order to obtain a detectable and / or quantifiable signal.
  • the antibodies according to the invention can be used in a method for the detection and / or identification of bacteria belonging to the species Listeria monocytogenes or to an associated microorganism in a biological sample, characterized in that it comprises the following steps: a) bringing the biological sample into contact with an antibody according to the invention; b) highlighting of the antigen-antibody complex possibly formed.
  • the antibodies according to the present invention can also be used in order to detect an expression of a gene of Listeria monocytogenes or of associated microorganisms.
  • the presence of the expression product of a gene recognized by an antibody specific for said expression product can be detected by the presence of an antigen-antibody complex formed after the contact of the Listeria monocytogenes strain or of the microorganism associated with an antibody according to the invention.
  • the bacterial strain used may have been "prepared", that is to say centrifuged, lysed, placed in a reagent suitable for constituting the medium suitable for the immunological reaction.
  • a method is preferred for detecting expression in the gene, corresponding to a Western blot, which can be carried out after electrophoresis on polyacrylamide gel of a lysate of the bacterial strain, in the presence or in the absence of reducing conditions (SDS-PAGE) . After migration and separation of the proteins on the polyacrylamide gel, said proteins are transferred to an appropriate membrane (for example made of nylon) and the presence of the protein or polypeptide of interest is detected, by contacting said membrane with a antibody according to the invention.
  • an appropriate membrane for example made of nylon
  • kits or kits necessary for the implementation of a method as described for detecting the expression of a gene of Listeria monocytogenes or an associated microorganism, or for the detection and / or the identification of bacteria belonging to the species Listeria monocytogenes or an associated microorganism
  • a method as described for detecting the expression of a gene of Listeria monocytogenes or an associated microorganism, or for the detection and / or the identification of bacteria belonging to the species Listeria monocytogenes or an associated microorganism
  • the present invention also comprises the kits or kits necessary for the implementation of a method as described (for detecting the expression of a gene of Listeria monocytogenes or an associated microorganism, or for the detection and / or the identification of bacteria belonging to the species Listeria monocytogenes or an associated microorganism), comprising the following elements: a) a polyclonal or monoclonal antibody according to the invention; b) optionally, the reagents for constituting the medium suitable for the immuno
  • polypeptides and antibodies according to the invention can advantageously be immobilized on a support, in particular a protein chip.
  • a protein chip is an object of the invention, and can also contain at least one polypeptide from a microorganism other than Listeria monocytogenes or an antibody directed against a compound of a microorganism other than Listeria monocytogenes.
  • the protein chips or high density filters containing proteins according to the invention can be constructed in the same way as the DNA chips according to the invention.
  • the latter method is preferable, when it is desired to attach proteins of large size to the support, which are advantageously prepared by genetic engineering.
  • the protein chips according to the invention can advantageously be used in kits or necessary for the detection and / or identification of bacteria associated with the species Listeria monocytogenes or with a microorganism, or more generally in kits or necessary for the detection and / or identification of microorganisms.
  • the polypeptides according to the invention are fixed on the DNA chips, the presence of antibodies is sought in the samples tested, the fixing of an antibody according to the invention on the support of the protein chip allowing the identification of the protein of which said antibody is specific.
  • an antibody according to the invention is fixed on the support of the protein chip, and the presence of the corresponding antigen, specific for Listeria monocytogenes or an associated microorganism, is detected.
  • a protein chip described above can be used for the detection of gene products, to establish an expression profile of said genes, in addition to a DNA chip according to the invention.
  • the protein chips according to the invention are also extremely useful for proteomics experiments, which studies the interactions between the different proteins of a given microorganism.
  • proteomics experiments which studies the interactions between the different proteins of a given microorganism.
  • peptides representative of the various proteins of an organism are fixed on a support. Then, said support is brought into contact with labeled proteins, and after an optional rinsing step, interactions between said labeled proteins and the peptides fixed on the protein chip are detected.
  • protein chips comprising a polypeptide sequence according to the invention or an antibody according to the invention are subject of the invention, as well as the kits or kits containing them.
  • the present invention also covers a method for detecting and / or identifying bacteria belonging to the species Listeria monocytogenes or to an associated microorganism in a biological sample, which implements a nucleotide sequence according to the invention.
  • biological sample relates in the present invention to samples taken from a living organism (in particular blood, tissues, organs or the like taken from a mammal) or a sample containing biological material, that is, DNA.
  • a biological sample therefore includes food compositions containing bacteria (for example cheeses, dairy products), but also food compositions containing yeasts (beers, breads) or others.
  • the detection and / or identification process using the nucleotide sequences according to the invention can be of various nature.
  • a method comprising the following steps: a) optionally, isolation of the DNA from the biological sample to be analyzed, or obtaining a cDNA from the RNA of the biological sample; b) specific amplification of the DNA of bacteria belonging to the species
  • This process is based on specific amplification of DNA, in particular by an amplification chain reaction.
  • a method is also preferred comprising the following steps: a) bringing a nucleotide probe according to the invention into contact with a biological sample, the nucleic acid contained in the biological sample having, if necessary, previously been made accessible to hybridization, under conditions allowing hybridization of the probe to the nucleic acid of a bacterium belonging to the species Listeria monocytogenes or to an associated microorganism; b) demonstration of the hybrid possibly formed between the nucleotide probe and the DNA of the biological sample.
  • Such a method should not be limited to the detection of the presence of the DNA contained in the certified biological sample, it can also be implemented to detect the RNA contained in said sample. This process includes in particular the Southern and Northern blot.
  • Another preferred method according to the invention comprises the following steps: a) bringing a nucleotide probe immobilized on a support according to the invention into contact with a biological sample, the nucleic acid of the sample, having, where appropriate , been previously made accessible to hybridization, under conditions allowing hybridization of the probe to the nucleic acid of a bacterium belonging to the species Listeria monocytogenes or to an associated microorganism; b) bringing the hybrid formed into contact between the nucleotide probe immobilized on a support and the nucleic acid contained in the biological sample, where appropriate after elimination of the DNA from the biological sample which has not hybridized with the probe, with a labeled nucleotide probe according to the invention; c) highlighting of the new hybrid formed in step b).
  • This method is advantageously used with a DNA chip according to the invention, the desired nucleic acid hybridizing with a probe present on the surface of said chip, and being detected by the use of a labeled probe.
  • This method is advantageously implemented by combining a prior step of amplification of the DNA or of the complementary DNA optionally obtained by reverse transcription, using primers according to the invention.
  • kits or kits for the detection and / or identification of bacteria belonging to the species Listeria monocytogenes or to an associated microorganism characterized in that it comprises the following elements: a) a nucleotide probe according to the invention; b) optionally, the reagents necessary for carrying out a hybridization reaction; c) optionally, at least one primer according to the invention as well as the reagents necessary for a DNA amplification reaction.
  • kits or kits for the detection and / or identification of bacteria belonging to the species Listeria monocytogenes or to an associated microorganism characterized in that it comprises the following elements: ) a nucleotide probe, called a capture probe, according to the invention; b) an oligonucleotide probe, called the revelation probe, according to the invention; c) optionally, at least one primer according to the invention as well as the reagents necessary for a DNA amplification reaction.
  • kits or kits for the detection and / or identification of bacteria belonging to the species Listeria monocytogenes or to an associated microorganism characterized in that it comprises the following elements: a) at least one primer according to the invention; b) optionally, the reagents necessary to carry out a DNA amplification reaction; c) optionally, a component making it possible to verify the sequence of the amplified fragment, more particularly an oligonucleotide probe according to the invention. are also objects of the present invention.
  • said primers and / or probes and / or polypeptides and / or antibodies according to the present invention used in the methods and / or kits or necessary according to the present invention are chosen from primers and / or probes and / or polypeptides and / or antibodies specific for the species Listeria monocytogenes.
  • these elements are chosen from the nucleotide sequences leading to a secreted protein, from secreted polypeptides, or from antibodies directed against secreted polypeptides of Listeria monocytogenes.
  • the present invention also relates to strains of Listeria monocytogenes and / or associated microorganisms containing one or more mutation (s) in a nucleotide sequence according to the invention, in particular an ORF sequence, or their regulatory elements (in particular promoters) .
  • strains of Listeria monocytogenes having one or more mutation (s) in the nucleotide sequences coding for polypeptides involved in the cellular machine, in particular secretion, central intermediate metabolism, energy metabolism, process of amino acid synthesis, transcription and translation, synthesis of polypeptides.
  • Said mutations can lead to inactivation of the gene, or in particular when they are located in the regulatory elements of said gene, to overexpression of the latter.
  • the invention further relates to the use of a nucleotide sequence according to the invention, a polypeptide according to the invention, an antibody according to the invention, a cell according to the invention, and / or d '' an animal transformed according to the invention, for the selection of organic or inorganic compound capable of modulating, regulating, inducing or inhibiting the expression of genes, and / or modifying the cellular replication of eukaryotic or prokaryotic cells or capable of inducing , to inhibit or aggravate the pathologies linked to an infection by Listeria monocytogenes or one of its associated microorganisms.
  • the invention also includes a method of selecting compounds capable of binding to a polypeptide or a fragment thereof according to the invention, capable of binding to a nucleotide sequence according to the invention, or capable of recognizing an antibody according to claim , and / or capable of modulating, regulating, inducing or inhibiting gene expression, and / or modifying the growth or cellular replication of eukaryotic or prokaryotic cells, or capable of inducing, inhibiting or aggravate in an animal or human organism the pathologies linked to an infection with Listeria monocytogenes, or one of its associated microorganisms, characterized in that it comprises the following steps: a) bringing said compound into contact with said polypeptide , said nucleotide sequence, with a cell transformed according to the invention and / or administration of said compound to an animal transformed according to the invention; b) determining the capacity of said compound to bind with said polypeptide or said nucleotide sequence, or to modulate, regulate, induce or inhibit the expression of genes, or to
  • the cells and / or animals transformed according to the invention can advantageously serve as a model and be used in methods for studying, identifying and / or selecting compounds liable to be responsible for pathologies induced or aggravated by Listeria monocytogenes, or susceptible to prevent and / or treat these pathologies such as for example genital, ocular or systemic diseases, in particular of the lymphatic system.
  • transformed host cells in particular bacteria of the Listeriae family, the transformation of which by a vector according to the invention can for example increase or inhibiting its infectious power, or modulating the pathologies usually induced or aggravated by the infection, can be used to infect animals whose pathologies will be monitored.
  • the animals transformed according to the invention may be used in methods of selecting compounds capable of preventing and / or treating diseases due to Listeria. Said methods using said transformed cells and / or transformed animals form part of the invention.
  • the compounds which can be selected can be organic compounds such as polypeptides or carbohydrates or any other organic or inorganic compounds already known, or new organic compounds produced from molecular modeling techniques and obtained by chemical or biochemical synthesis. , these techniques being known to those skilled in the art.
  • Said selected compounds may be used to modulate the growth and / or cell replication of Listeria monocytogenes or any other associated microorganism and thus to control infection by these microorganisms.
  • Said compounds according to the invention may also be used to modulate the growth and / or cell replication of all eukaryotic or prokaryotic cells, in particular tumor cells and infectious microorganisms, for which said compounds will prove to be active, the methods making it possible to determine said modulations being well known to those skilled in the art.
  • the term “compound capable of modulating the growth of a microorganism” is intended to denote any compound which makes it possible to intervene, modify, limit and / or reduce the development, growth, rate of proliferation and / or viability of said aforementioned. microorganism.
  • This modulation can be carried out for example by an agent capable of binding to a protein and thus of inhibiting or potentiating its biological activity, or capable of binding to a membrane protein of the external surface of a microorganism and of blocking the penetration of said microorganism into the host cell or to promote the action of the immune system of the infected organism directed against said microorganism.
  • This modulation can also be carried out by an agent capable of binding to a nucleotide sequence of a DNA or RNA of a microorganism and of blocking for example the expression of a polypeptide whose biological or structural activity is necessary for growth or reproduction of said microorganism.
  • associated microorganism is intended to denote any microorganism whose gene expression can be modulated, regulated, induced or inhibited, or whose cell growth or replication can also be modulated by a compound of l 'invention. It is also intended to denote by associated microorganism in the present invention any microorganism comprising nucleotide sequences or polypeptides according to the invention. These microorganisms can in certain cases contain polypeptides or nucleotide sequences identical or homologous to those of the invention can also be detected and / or identified by the methods or kit of detection and / or identification according to the invention and also serve of target for the compounds of the invention.
  • the invention relates to compounds capable of being selected by a selection method according to the invention.
  • the invention also relates to a pharmaceutical composition
  • a pharmaceutical composition comprising a compound chosen from the following compounds: a) a nucleotide sequence according to the invention; b) a polypeptide according to the invention; c) a vector according to the invention; d) an antibody according to the invention; and e) a compound capable of being selected by a selection method according to the invention, optionally in combination with a pharmaceutically acceptable vehicle.
  • effective quantity is intended to denote a sufficient quantity of the said compound or antibody, or of the polypeptide of the invention, making it possible to modulate the growth of Listeria monocytogenes or of an associated microorganism.
  • the invention also relates to a pharmaceutical composition according to the invention for the prevention or treatment of an infection by a bacterium belonging to the species Listeria monocytogenes or by an associated microorganism.
  • the invention further relates to an immunogenic and / or vaccine composition, characterized in that it comprises one or more polypeptides according to the invention and / or one or more hybrid polypeptides according to the invention.
  • the invention also includes the use of a transformed cell according to the invention, for the preparation of a vaccine composition.
  • the invention also relates to a vaccine composition, characterized in that it contains a nucleotide sequence according to the invention, a vector according to the invention and / or a transformed cell according to the invention.
  • the invention also relates to the vaccine compositions according to the invention, for the prevention or treatment of an infection by a bacterium belonging to the species Listeria monocytogenes or by an associated microorganism.
  • the immunogenic and / or vaccine compositions according to the invention intended for the prevention and / or treatment of infection by Listeria monocytogenes or by an associated microorganism will be chosen from the immunogenic and / or vaccine compositions comprising a polypeptide or one of its fragments corresponding to a protein, or one of its fragments, of the cell envelope of Listeria monocytogenes.
  • the vaccine compositions comprising nucleotide sequences will preferably also comprise nucleotide sequences coding for a polypeptide or one of its fragments corresponding to a protein, or one of its fragments, of the cell envelope of Listeria monocytogenes.
  • immunogenic and / or vaccine compositions the most preferred are those comprising a polypeptide or one of its fragments, or a nucleotide sequence or one of its fragments, the sequences of which are chosen from the nucleotide or amino acid sequences identified in this functional group and listed above.
  • polypeptides of the invention or their fragments entering into the immunogenic compositions according to the invention can be selected by techniques known to those skilled in the art, for example on the capacity of said polypeptides to stimulate T cells, which is reflected for example by their proliferation or the secretion of interleukins, and which results in the production of antibodies directed against said polypeptides.
  • the antibody reaction is tested by sampling the serum followed by a study of the formation of a complex between the antibodies present in the serum and the antigen of the vaccine composition, according to the usual techniques.
  • said vaccine compositions will preferably be in association with a pharmaceutically acceptable vehicle and, where appropriate, with one or more appropriate immunity adjuvants.
  • This type of vaccination is carried out with a particular plasmid derived from an E. coli plasmid which does not replicate in vivo and which codes only for the vaccinating protein. Animals have been immunized by simply injecting naked plasmid DNA into the muscle. This technique leads to the expression of the vaccine protein in situ and to an immune response of cell type (CTL) and of humoral type (antibody). This double induction of the immune response is one of the main advantages of the vaccination technique with naked DNA.
  • CTL cell type
  • antibody humoral type
  • the vaccine compositions comprising nucleotide sequences or vectors into which said sequences are inserted, are in particular described in international application No. WO 90/11092 and also in international application No. WO 95/11307.
  • the nucleotide sequence constituting the vaccine composition according to the invention can be injected into the host after being coupled to compounds which promote the penetration of this polynucleotide inside the cell or its transport to the cell nucleus.
  • the resulting conjugates can be encapsulated in polymer microparticles, as described in international application No. WO 94/27238 (Medisorb Technologies International).
  • the nucleotide sequence preferably a DNA
  • the nucleotide sequence is complexed with DEAE-dextran, with nuclear proteins, with lipids or encapsulated in liposomes or even introduced under the form of a gel facilitating its transfection into cells.
  • the polynucleotide or the vector according to the invention can also be in suspension in a buffer solution or be associated with liposomes.
  • such a vaccine will be prepared according to the technique described by Tacson et al. or Huygen et al. in 1996 or according to the technique described by Davis et al. in international application No. WO 95/11307.
  • Such a vaccine can also be prepared in the form of a composition containing a vector according to the invention, placed under the control of regulatory elements allowing its expression in humans or animals. It will be possible, for example, to use, as an in vivo expression vector for the polypeptide antigen of interest, the plasmid pcDNA3 or the plasmid pcDNAl / neo, both marketed by Invitrogen (R & D Systems, Abingdon, United Kingdom). United). Such a vaccine will advantageously comprise, in addition to the recombinant vector, a saline solution, for example a sodium chloride solution.
  • a saline solution for example a sodium chloride solution.
  • pharmaceutically acceptable vehicle is intended to denote a compound or a combination of compounds entering into a pharmaceutical or vaccine composition which does not cause side reactions and which allows for example the facilitation of the administration of the active compound, the increase in its duration of life and / or its efficiency in the organism, increasing its solubility in solution or improving its conservation.
  • pharmaceutically acceptable vehicles are well known and will be adapted by those skilled in the art depending on the nature and the mode of administration of the active compound chosen.
  • these can include suitable immunity adjuvants which are known to those skilled in the art, such as, for example, aluminum hydroxide, a representative of the family of muramyl peptides. as one of the peptide derivatives of N-acetyl-muramyl, a bacterial lysate, or even the incomplete adjuvant of Freund.
  • these compounds will be administered by the systemic route, in particular by the intravenous route, by the intramuscular, intradermal or subcutaneous route, or by the oral route. More preferably, the vaccine composition comprising polypeptides according to the invention will be administered several times, over a period of time, by the intradermal or subcutaneous route.
  • dosages and dosage forms can be determined according to the criteria generally taken into account in establishing a treatment adapted to a patient such as for example the patient's age or body weight, the severity of his general condition, tolerance to treatment and side effects observed.
  • the invention comprises the use of a composition according to the invention, for the treatment or prevention of genital diseases, induced or aggravated by Listeria monocytogenes.
  • the invention comprises the use of a composition according to the invention, for the treatment or prevention of diseases induced or aggravated by the presence of Listeria monocytogenes.
  • the invention comprises the use of a composition according to the invention, for the treatment or prevention of systemic diseases, in particular of the lymphatic system, induced or aggravated by the presence of Listeria monocytogenes.
  • the present invention also relates to a genomic DNA library of a bacterium of the genus Listeria, preferably, Listeria monocytogenes, preferably the strain EGD-e, said DNA library being cloned into chromosomes artificial bacteria (BAC).
  • a genomic DNA library contains very large inserts of the Listeria genome, in particular inserts with a size of between 50 and 200 kb.
  • the genomic DNA bank described in the present invention indeed covers the genome of Listeria monocytogenes.
  • these regions can easily be amplified and identified by a person skilled in the art, using oligonucleotides specific for the sequences of the sequences. ends of the different clones which form the contigs.
  • the present invention also relates to methods for the isolation of a polynucleotide of interest present in a strain of Listeria and absent in another strain, which uses at least one DNA library based on a BAC, containing the Listeria genome .
  • the method according to the invention for the isolation of a polynucleotide of interest can comprise the following steps: a) isolating at least one polynucleotide contained in a clone of the DNA library based on a BAC, of listeria origin , b) isolate:
  • At least one genomic polynucleotide or cDNA of a listeria said listeria belonging to a strain different from the strain used for the construction of the BAC DNA library of step a) or, alternatively, at least one polynucleotide contained in a clone of a DNA library based on a BAC prepared from the genome of a listeria which is different from the listeria used for the construction of the DNA library based on the BAC of step a) .
  • step a) hybridizing the polynucleotide of step a) to the polynucleotide of step b); d) selecting the polynucleotides of step a) which have not formed a hybridization complex with the polynucleotides of step b); e) characterize the selected polynucleotide.
  • the polynucleotide of step a) can be prepared by digestion of at least one recombinant BAC clone with an appropriate restriction enzyme, and optionally, the amplification of the resulting polynucleotide.
  • the method of the invention allows a person skilled in the art to carry out comparative genomic studies between the different strains or species of the genus listeria, for example between the pathogenic strains and their non-pathogenic equivalent.
  • the blocks containing the chromosomal DNA of Listeria monocytogenes, with an average weight of 80 mg were prepared in agarose, by methods known to those skilled in the art. They were kept in a 500 mM EDTA solution, pH 8.0.
  • Partial digestions are carried out in a digestion buffer (2 mMspermidine, 0.5 mM DTT, 0.02 ⁇ g BSA, restriction buffer 0.5x) for 30 min at 4 ° C, without restriction enzyme, then added for 2 h at 37 ° C, 0.1; 0.25; 0.4 or 0.5 units of EcoRI (life Tech) per tube. Partial digestion is stopped by replacing the digestion buffer with 200 ⁇ l 0.5 M EDTA, the tubes being placed on ice. The agarose blocks are then placed on a 1% SeaKem GTG agarose gel (FMC), 0.5 ⁇ TBE buffer. The electrophoresis gel is then carried out in pulsed fields with the following conditions: initial pulse time: 90 seconds, final pulse time: 90 seconds,
  • the region between 50 and 200 kb is cut from the gel, and it is separated into three pieces (50-100 kb, 100-150 kb, 150-200 kb).
  • the cloning gel should not be stained with etidium bromide.
  • agarose blocks are placed in a new 1% SeaKem GTG agarose gel (FMC), 0.5x TBE buffer.
  • FMC SeaKem GTG agarose gel
  • a new electrophoresis gel is carried out in pulsed fields with the following conditions: initial pulse time: 5 seconds, final pulse time: 5 seconds,
  • the agarose pieces are cut into small pieces of about 100 mg each.
  • the agarose is incubated at 67 ° C for 10 min then cooled to 42 ° C, and 1 ⁇ l of Beta-Agarase (FMC, lU / ⁇ l) is added. Incubate for 30 min at 42 ° C, and denaturation of the Beta-Agarase is carried out after complete digestion of the agarose by incubation for 10 min at 67 ° C, then incubation on ice.
  • Beta-Agarase FMC, lU / ⁇ l
  • 150 ng of DNA vectors digested with EcoRI and phophorylates are used for the construction of the BAC library.
  • the vector pBelaBAC-Kan (Mozo et al., Mol. Gen. Genêt., 1998, 258, 562-70) is used for the construction of said library.
  • the ligation buffer is the buffer recommended by the manufacturer.
  • the transformation is carried out by adding 5 ⁇ l of the ligation reaction to 40 ⁇ l of DH10B electrocompetent cells, and the electroporation is carried out in a Life Tech electroporator with the following conditions:
  • Bioinformatics plays a key role in the three phases of a genome project: follow-up of the inserts produced by the random sequencing method, the finishing phase of the genome sequence, and annotations.
  • the Inventors have developed a complete software which makes it possible to meet these three requirements: GMP-Tool-box (GMPTB).
  • GMPTB extracts from the result files (Phrap format) all the characteristics necessary for the assembly (number of contigs, number of sequences ...) and displays them in a table. This table can be used to create graphs that show the progress of this method and allow quick identification of assembly problems. Importantly, GMPTB allows the comparison between the assembly results and creates an HTML page in order to describe the relationship between the new and the old contigs (fusion, creation ).
  • GMPTB Finishing phase of the sequence: Different strategies are used by GMPTB to predict the links between the contigs. GMPTB searches in particular all the clones allowing the links, on the basis of the localization and the orientation of the terminal sequences. It can also indicate misassemblies.
  • GMPTB can also predict linkages, based on genome comparisons, by looking for similarities between the ends of the contigs and other genomic sequences (high nucleotide and amino acid level).
  • GMPTB allows you to start annotation during the terminal phase.
  • GMPTB creates an individual protein file (IPF), for each open reading phase (ORF) during assembly.
  • IPF individual protein file
  • ORF open reading phase
  • the minimum fields contain an identification number, a version number, the location and the sequences.
  • the exported nucleotide sequence corresponds to the sequence of the open reading phase with 500 additional bases before the first stop codon and 200 additional bases after the second stop.
  • the automatic field contains results added by different programs to the IPF. It concerns the DNA sequence (search for ribosome binding sites, promoters or terminators, coding capacity, etc.) and the predicted protein sequence (homology, domain, etc.).
  • IPFs are linked to a Sybases genomic database (of the SubtiList model) and are accessible through a web server. They can be modified and annotated by different Inventors during the genome project phase. Deposit of biological material
  • Said BAC bank (1-2439) was produced in the E.coli DH10B strain (Grant et al., PNAS, 87, 4645, 1990), constructed after partial digestion of the DNA of Listeria monocytogenes with the enzyme EcoRI in the vector pBelaBAC-Kan (Mozo et al., Mol. Gen. Genêt., 1998, 258, 562-70).
  • SeqlD n ° 39 LM-894.1 From 2469093 to 2471915 Unknown, similar to internalin proteins SeqlD n ° 40 LM-966.1 From 173309 to 174556 Unknown, cell wall anchored protein SeqlD n ° 41 LM-973.1 From 169510 to 172008 Unknwon, similar to internalin proteins SeqlD n ° 42 L -133.1 From 1230773 to 1232308 Unknown, similar to cobyric acid synthase CbiP SeqlD n ° 43 LM-134.1 From 1229967 to 1230773 Unknown, similar to cobalt transport ATP-binding protein CbiO
  • SeqlD # 109 LM-1060.1 From 2897116 to 2897823
  • Unknown SeqlD # 110 LM-1061.1 From 2896241 to 2897059 unknown, similar to D- alanyl-D-alanine carboxypeptidase
  • SeqlD n ° 172 LM-1139.1 From 790662 to 791960 Unknown SeqlD n ° 173 LM-114.1 From 2784805 to 2786319 Unknown, highiy similar to gluconate kinase
  • SeqlD n ° 194 LM-1165.1 From 772996 to 773700 Unknown SeqlD n ° 195 LM-1166.1 From 772310 to 772999 Unknown, similar to ABC transporter, ATP-binding protein
  • SeqlD n ° 212 From 1239842 to 1240201 unknown SeqlD n ° 213 LM-1190.1 From 2918048 to 2919169 unknown; similar to unknown proteins
  • SeqlD n ° 224 LM-1209.1 From 2930479 to 2932473 unknown SeqlD n ° 225 LM-121.1 From 1239039 to 1239797 unknown, similar to rRNA methylase
  • SeqlD n ° 235 LM-1222.1 From 2941083 to 2941544 unknown SeqlD n ° 236 LM-1223.2 From 2941599 to 2942219 unknown, highiy similar to
  • SeqlD n ° 263 LM-1267.1 From 848126 to 848788 unknown SeqlD n ° 264 LM-1268.1 From 847552 to 848109 unknown, some similarity to acatyltransferases
  • SeqlD n ° 292 LM-1304.1 From 920452 to 920928 unknown SeqlD n ° 293 LM-1306.1 From 918135 to 918902 unknown SeqlD n ° 294 LM-1307.1 From 917164 to 918117 unknown, similar to oxidoreductases
  • SeqlD n ° 303 LM-1319.1 From 910173 to 910484 unknown SeqlD n ° 304 LM-1320.1 From 909159 to 910157 unknown SeqlD n ° 305 LM-1321.1 From 908499 to 909056 unknown SeqlD n ° 306 LM-1322.1 From 907979 to 908467 unknown SeqlD n ° 307 LM-1325.1 From 905962 to 907524 Unknown, similar to ATP- dependent RNA helicase
  • SeqlD n ° 309 LM-1327.1 From 902773 to 903840 unknown SeqlD n ° 310 LM-1328.1 From 901837 to 902751 unknown SeqlD n ° 311 LM-1329.1 From 900303 to 901835 unknown, similar to oligo-
  • SeqlD n ° 380 LM-1438.1 From 212366 to 212689 Unknwon SeqlD n ° 381 LM-1439.1 From 211425 to 212294 phospholipase C SeqlD n ° 382 LM-1442.1 From 209470 to 211389 actin-assembly inducing protein precursor
  • SeqlD n ° 384 LM-1445.1 From 205819 to 207408 listeriolysin O precursor SeqlD n ° 385 LM-1446.1 From 204624 to 205577 phosphatidylinositol- specific phospholipase c
  • SeqlD n ° 410 LM-1480.1 From 1916166 to 1917452 unknown, highiy similar to uracil permease SeqlD n ° 411 LM-1481.2 From 1917581 to 1918132 unknown, highiy similar to pyrimidine operon regulatory protein
  • SeqlD n ° 412 LM-1482.2 From 1918363 to 1918593 unknown SeqlD n ° 413 LM-1483.2 From 645397 to 645858 Unknown, similar to transcription regulator
  • SeqlD n ° 432 LM-1510.3 From 661470 to 662171 Unknown SeqlD n ° 433 LM-1511.3 From 662257 to 663936 Unknown, similar to unknown protein
  • SeqlD n ° 434 LM-1516.1 From 669423 to 669950 Unknown SeqlD n ° 435 LM-1518.1 From 670309 to 672339 Unknown, similar to transcription antiterminator
  • SeqlD n ° 443 LM-1528.1 From 678494 to 679123 Unknown SeqlD n ° 444 LM-1529.1 From 679763 to 680296 Unknown, similar to a transcription regulator
  • SeqlD n ° 465 LM-156.1 From 1213636 to 1215087 unknown SeqlD n ° 466 LM-1560.1 From 447471 to 447791 Unknown, similar to PTS fructose-specific enzyme MB component
  • SeqlD n ° 481 LM-1579.1 From 976065 to 977039 Unknown SeqlD n ° 482 LM-158.1 From 1211869 to 1212990 Unknown, similar to ethanolamine utilization protein EutH - Escherichia coli SeqlD n ° 483 LM-1580.1 From 975071 to 976033 Unknown
  • SeqlD n ° 504 LM-1605.1 From 957724 to 958764 unknown, similar to B. subtilis YcgR protein SeqlD n ° 505 LM-1606.1 From 956031 to 957602 unknown, similar to ABC transporter ATP-binding protein (antibiotic resistance)
  • SeqlD n ° 509 LM-161.1 From 1210459 to 1211031 unknown SeqlD n ° 510 LM-1610.1 From 950673 to 951941 Unknown, similar to phosphotransferase system enzyme IIC
  • SeqlD n ° 527 LM-163.1 From 1209053 to 1209808 unknown, similar to cobalamin adenosyl transferase SeqlD n ° 528 LM-1631.1 From 473936 to 476716 Unknown, conserved hypothetical protein SeqlD n ° 529 LM-1632.1 From 476960 to 478447 Unknown, similar to transcription regulator SeqlD n ° 530 LM-1634.1 From 478721 to 479710 Unknown, similar to penicillin acylase and to conjugated bile acid hydrolase
  • SeqlD # 535 LM-1639.1 From 484620 to 485375 Unknown SeqlD # 536 LM-164.1 From 1208603 to 1208887 unknown, similar to putative carboxysome structural protein
  • SeqlD n ° 551 LM-1663.1 From 2630285 to 2631310 Unknown, similar to galactosyltransferase SeqlD n ° 552 LM-1664.1 From 2629208 to 2630278 Unknown, conserved hypothetical protein SeqlD n ° 553 LM-1665.1 From 2627816 to 2629087 unknown, highiy similar to
  • SeqlD n ° 559 LM-1674.1 From 2622067 to 2623122 unknown, highiy similar to threonine synthase SeqlD n ° 560 LM-1676.1 From 2621201 to 2622067 unknown, highiy similar to homoserine kinase SeqlD n ° 561 LM-1678.1 From 2620514 to 2621089 Unknwon, similar to thymidine kinase SeqlD n ° 562 LM-1679.1 From 2619415 to 2620491 unknown, highiy similar to peptide chain release factor 1
  • SeqlD # 566 LM-1683.1 From 2616835 to 2617242 Unknown, similar to phosphatases SeqlD # 567 LM-1684.1 From 2615458 to 2616699 unknown, highiy similar to glycine hydroxymethyltransferase
  • SeqlD n ° 600 LM-1726.1 From 539365 to 539763 unknown SeqlD n ° 601 LM-1727.1 From 539774 to 540424 Unknown, similar to ribulose-5-phosphate 3- epimerase
  • SeqlD n ° 644 LM-1790.1 From 1454347 to 1455177 Unknown SeqlD n ° 645 LM-1791.2 From 1455316 to 1456662 Unknown, similar to metal ion transport proteins
  • SeqlD n ° 652 LM-1800.2 From 1461343 to 1461726 Unknown, similar to unknown proteins SeqlD n ° 653 LM-1801.2 From 1461866 to 1463467 Unknown, similar to ABC transporter (ATP-binding protein)
  • SeqlD # 684 LM-1840.1 From 284365 to 286011 internalin H SeqlD # 685 LM-1842.1 From 282755 to 284227 internalin G SeqlD # 686 LM-1843.1 From 281021 to 282481 Unknown, similar to phospho-beta-glucosidase
  • SeqlD n ° 726 LM-190.1 From 1189785 to 1190264 Unknown SeqlD n ° 727 LM-1900.1 From 2207103 to 2208362 Unknown, similar to maltose / maltodextrin- binding protein
  • SeqlD n ° 736 LM-1913.1 From 2196382 to 2197206 unknown SeqlD n ° 737 LM-1915.1 From 2194399 to 2196339 Unknown, similar to ABC transporter (permease)
  • SeqlD n ° 749 LM-1932.1 From 2182218 to 2182784 Unknown SeqlD n ° 750 LM-1933.2 From 2181329 to 2182216 Unknown, similar to a protein required for pyridoxine synthesis
  • SeqlD n ° 755 LM-1944.1 From 1616945 to 1617880 Unknown, similar to unknown proteins SeqlD n ° 756 LM-1945.1 From 1617901 to 1619214 Unknown, similar to unknown proteins SeqlD n ° 757 LM-1946.1 From 1619240 to 1619926 Unknown, similar to unknown proteins SeqlD n ° 758 LM-1947.1 From 1620091 to 1621188 Unknown, similar to X-Pro dipeptidase SeqlD n ° 759 LM-1948.1 From 1621230 to 1622342 Unknown, similar to alanine dehydrogenase SeqlD n ° 760 LM-1949.1 From 1622583 to 1623047 Unknown, similar to unknown protein SeqlD n ° 761 LM-195.1 From 1188293 to 1188928 unknown, similar to
  • SeqlD n ° 785 LM-1981.1 From 1323450 to 1323683 Unknown, similar to host factor-1 protein SeqlD n ° 786 LM-1983.1 From 1323794 to 1325017 unknown, conserved hypothetical protein similar to B. subtilis YnbA protein
  • SeqlD n ° 789 LM-1986.1 From 1326879 to 1328213 unknown, highiy similar to glutamine synthetases SeqlD n ° 790 LM-1988.1 From 1328357 to 1329652 Unknown, similar to arsenic efflux pump protein SeqlD n ° 791 LM-1989.1 From 1329696 to 1330217 unknown, conserved hypothetical protein SeqlD n ° 792 LM-1991.2 From 1330247 to 1330861 unknown, highiy similar to
  • SeqlD n ° 796 LM-1997.1 From 1334318 to 1335160 unknown SeqlD n ° 797 LM-1998.1 From 1335179 to 1335910 unknown, weakiy similar to arginine N- methyltransferases

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Abstract

La présente invention concerne la séquence génomique et des séquences nucléotidiques de Listeria monocytogenes EGD-e. L'invention a également pour objet les polypeptides de cet organisme, en particulier les polypeptides de surface ou d'enveloppe cellulaire, ou impliqués dans les différents cycles de métabolisme, en particulier la biosynthèse de vitamine B12. L'invention concerne aussi les utilisations des séquences décrites, ainsi que différents outils permettant l'identification de L. monocytogenes ou espèces associées. L'invention concerne ausi des souches L. monocytogenes modifiées, ainsi que les compositions pharmaceutiques ou vaccinales utilisant les séquences de l'invention.

Description

GENOME DE Listeria monocytogenes, POLYPEPTIDES ET UTILISATIONS
L'invention a pour objet la séquence génomique et des séquences nucléotidiques codant pour des polypeptides de Listeria monocytogenes, tels que des polypeptides d'enveloppe cellulaire, sécrétés ou spécifiques, ou impliqués dans le métabolisme, dans le processus de réplication ou dans la virulence, ainsi que des vecteurs incluant lesdites séquences et cellules ou animaux transformés par ces vecteurs. L'invention concerne également des procédés de détection de ces acides nucléiques ou polypeptides et des kits de diagnostic d'infection par Listeria monocytogenes. L'invention vise aussi une méthode de sélection de composés capables de moduler l'infection bactérienne et un procédé de biosynthèse ou de biodégradation de molécules d'intérêt utilisant lesdites séquences nucléotidiques ou lesdits polypeptides. L'invention comprend enfin des compositions pharmaceutiques, notamment vaccinales, pour la prévention et/ou le traitement d'infections bactériennes, en particulier par Listeria monocytogenes.
Listeria monocytogenes est un pathogène intracellulaire facultatif. Il s'agit de l'agent étio logique de la listériose, une infection liée à la nourriture posant des problèmes de santé publique de plus en plus importants, avec un impact économique important pour l'industrie alimentaire européenne. La listériose est l'infection liée aux aliments la plus létale (mortalité d'environ 30 %). Listeria monocytogenes possède la propriété inhabituelle d'être capable de traverser trois barrières : La barrière intestinale, la barrière hémato-encéphalique et la barrière placentaire. Les manifestations cliniques de la listériose incluent les méningites, méningo-encéphalites, avortements et septicémies. Cette infection est opportuniste et affecte principalement les femmes enceintes, les bébés, les personnes âgées et les personnes immuno-déprimées en particulier les personnes atteintes du SIDA. Cette maladie affecte apparemment également les individus sains et est responsable d'un nombre important d'épidémies en raison de produits alimentaires contaminés. Listeria monocytogenes est également d'une importance vétérinaire avec un risque principal pour les ovins (moutons) et les bovins. Listeria monocytogenes est particulièrement résistante au stress ou aux conditions extrêmes et il est important de rechercher sa présence avec soin non seulement pour des problèmes de sécurité alimentaire mais également pour des problèmes de sécurité environnementale.
La carte physique et une carte génétique préliminaire du génome de Listeria monocytogenes ont été établies pour la souche LO28. Néanmoins, aucune carte génétique fine n'est disponible pour l'instant. Le génome de cette bactérie est circulaire et comporte environ 3000 kilobases. Son contenu en GC est d'environ 38 %. Les études des facteurs de virulence ont permis l'identiFiç tïori d'un locus de 15 kb qui peut être considéré comme étant un îlot de pathogénicité dans la mesure où il contient la plupart des gènes dont la fonction dans la virulence a été clairement identifiée. En plus de ce locus, quelques autres gènes ont été identifiés en particulier les gènes d'invasion et de motilité et des gènes qui codent pour une hydrolase muréine, une supéroxide dismutase, des facteurs sigma, etc..
Une famille importante de protéines de Listeria monocytogenes est la famille des protéines de surface. Les processus d'évolution ont permis le développement d'un nombre de mécanismes uniques sur les bactéries Gram+, par lesquels elles peuvent immobiliser des protéines à leur surface. Les fonctions de ces différentes protéines de parois cellulaires sont extrêmement diverses. Pourtant, beaucoup de protéines liées de façon covalente à la surface des pathogènes Gram+ sont estimées être importantes pour la survie du pathogène à l'intérieur de l'hôte infecté. Pour Listeria monocytogenes, la capacité à pénétrer dans les cellules eukaryotes a été liée à une famille de protéines de surface et/ou sécrétées, les internalines. Jusqu'ici, neuf membres de la famille des internalines ont été identifiés (InlA, InlB, InlC, InlC2, InlD, InlE, InlF, InlG, InlH). On pense qu'elles sont ancrées dans la paroi cellulaire par un clivage protéolytique de la liaison T-G du motif LPxTG et que la liaison covalente du groupe carboxylique de la thréonine à un groupe amino libre dans le peptidoglycane. Des études récentes ont montré qu'il y avait deux classes d' internalines ; un groupe de protéines associées à la paroi cellulaire de haut point moléculaire (> 50 kDa) et un groupe de plus petites protéines (< 30 kDa) qui sont sécrétées. Ces deux classés possèdent des motifs similaires LRR très homologues, ainsi que les régions flanquantes LRR, et la séquence peptide-signal N-terminale. Les petites internalines (s-inl) ne possèdent pas la région répétée B et la séquence d'ancrage dans la paroi cellulaire et sont ainsi sécrétées. L'étude de Listeria monocytogenes demande de nouvelles approches, en particulier génétiques, afin d'améliorer la compréhension .des , différentes voies métaboliques de cet organisme.
Ainsi, c'est un objet de la présente invention que de divulguer la séquence complète du génome de Listeria monocytogenes EGD-e déposée à la CNCM le 11 avril 2000 sous le numéro 1-2440 ainsi que de tous les gènes contenus dans cedit génome.
En effet, la connaissance du génome de cet organisme permet de mieux définir les interactions entre les différents gènes, les différentes protéines, et par là- même, les différentes voies métaboliques. En effet, et contrairement à la divulgation de séquences isolées, la séquence génomique complète d'un organisme forme un tout, permettant d'obtenir immédiatement toutes les informations nécessaires à cet organisme pour croître et fonctionner. ; - .
La présente invention concerne donc une séquence nucléotidique de Listeria monocytogenes caractérisée en ce qu'elle correspond à la séquence SEQ ID N° 1.
La présente invention concerne également une séquence nucléotidique de Listeria monocytogenes caractérisée en ce qu'elle est choisie parmi : a) une séquence nucléotidique comportant au moins 80 %, 85 %, 90 %, 95 % ou 98 % d'identité avec SEQ ID N° 1 ; b) une séquence nucléotidique hybridant dans des conditions de forte stringence avec SEQ ID N° 1 ; c) une séquence nucléotidique complémentaire de SEQ ID N° 1 ou complémentaire d'une séquence nucléotidique telle que définie en a), ou b), ou une séquence nucléotidique de l'ARN correspondant ; d) une séquence nucléotidique de fragment représentatif de SEQ ID N° 1, ou de fragment représentatif d'une séquence nucléotidique telle que définie en a), b) ou c); e) une séquence nucléotidique comprenant une séquence telle que définie en a), b), c) ou d) ; et f) une séquence nucléotidique modifiée d'une séquence nucléotidique telle que définie en a), b), c), d) ou e). De façon plus particulière, la présente invention a également pour objet les séquences nucléotidiques caractérisées en ce qu'elles sont issues de SEQ ID N° 1 et en ce qu'elles codent pour un polypeptide choisi parmi les polypeptides de séquence SEQ ID N° 2 à SEQ ID N° 2854, de préférence codant pour un polypeptide d'enveloppe cellulaire ou présent à la surface de Listeria monocytogenes de séquence SEQ ID N° 2 à SEQ ID N° 41 ou codant pour un polypeptide impliqué dans la biosynthèse de la vitamine B12 de séquence SEQ ID N° 42 à SEQ ID N°64. La présente invention concerne aussi de façon plus générale les séquences nucléotidiques issues de SEQ ID N° 1, et codant pour un polypeptide de L. monocytogenes, telles qu'elles peuvent être isolées à partir de SEQ ID N° 1.
De plus, les séquences nucléotidiques caractérisées en ce qu'elles comprennent une séquence nucléotidique choisie parmi : a) une séquence nucléotidique codant pour un polypeptide choisi parmi les séquences SEQ ID N° 2 à SEQ ID N°2854, de préférence choisi parmi les polypeptides de séquence SEQ ID N° 2 à SEQ ID N° 41 ou SEQ ID N° 42 à SEQ ID N° 64 ; b) une séquence nucléotidique comportant au moins 80 %, 85 %, 90 %, 95 % ou 98 % d'identité avec une séquence nucléotidique codant pour un polypeptide choisi parmi les séquences SEQ ID N° 2 à SEQ ID N° 2854, de préférence choisi parmi les polypeptides de séquence SEQ ID N° 2 à SEQ ID N° 41 ou SEQ ID N° 42 à SEQ ID N° 64 ; c) une séquence nucléotidique s'hybridant dans des conditions de forte stringence avec une séquence nucléotidique codant pour un polypeptide choisi parmi les séquences SEQ ID N° 2 à SEQ ID N° 2854, de préférence choisi parmi les polypeptides de séquence SEQ ID N° 2 à SEQ ID N° 41 ou SEQ ID N° 42 à SEQ ID N° 64 ; d) une séquence nucléotidique complémentaire ou d'ARN correspondant à une séquence telle que définie en a), b) ou c) ; e) une séquence nucléotidique de fragment représentatif d'une séquence telle que définie en a), b), c) ou d) ; et f) une séquence nucléotidique modifiée d'une séquence telle que définie en a), b), c), d) ou e), sont également des objets de l'invention.
Par acide nucléique, séquence nucléique ou d'acide nucléique, polynucléotide, oligonucléotide, séquence de polynucléotide, séquence nucléotidique, termes qui seront employés indifféremment dans la présente description, on entend désigner un enchaînement précis de nucléotides, modifiés ou non, permettant de définir un fragment ou une région d'un acide nucléique, comportant ou non des nucléotides non naturels, et pouvant correspondre aussi bien à un ADN double brin, un ADN simple brin que des produits de transcription desdits ADNs. Ainsi, les séquences nucléiques selon l'invention englobent également les PNA (Peptid Nucleic Acid), ou analogues.
Il doit être compris que la présente invention ne concerne pas les séquences nucléotidiques dans leur environnement chromosomique naturel, c'est-à-dire à l'état naturel. Il s'agit de séquences qui ont été isolées et/ou purifiées, c'est-à-dire qu'elles ont été prélevées directement ou indirectement, par exemple par copie, leur environnement ayant été au moins partiellement modifié. On entend ainsi également désigner les acides nucléiques obtenus par synthèse chimique.
Par « pourcentage d'identité » entre deux séquences d'acides nucléiques ou d'acides aminés au sens de la présente invention, on entend désigner un pourcentage de nucléotides ou de résidus d'acides aminés identiques entre les deux séquences à comparer, obtenu après le meilleur alignement, ce pourcentage étant purement statistique et les différences entre les deux séquences étant réparties au hasard et sur toute leur longueur. On entend désigner par "meilleur alignement" ou "alignement optimal", l'alignement pour lequel le pourcentage d'identité déterminé comme ci-après est le plus élevé. Les comparaisons de séquences entre deux séquences d'acides nucléiques ou d'acides aminés sont traditionnellement réalisées en comparant ces séquences après les avoir alignées de manière optimale, ladite comparaison étant réalisée par segment ou par « fenêtre de comparaison » pour identifier et comparer les régions locales de similarité de séquence. L'alignement optimal des séquences pour la comparaison peut être réalisé, outre manuellement, au moyen de l'algorithme d'homologie locale de Smith et Waterman (1981, Ad. App. Math. 2 : 482), au moyen de l'algorithme d'homologie locale de Neddleman et Wunsch (1970, J. Mol. Biol. 48 : 443), au moyen de la méthode de recherche de similarité de Pearson et Lipman (1988, Proc. Natl. Acad. Sci. USA 85 : 2444), au moyen de logiciels informatiques utilisant ces algorithmes (GAP, BESTFIT, BLAST P, BLAST N, FASTA et TFASTA dans le Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, WI). Afin d'obtenir l'alignement optimal, on utilise de préférence le programme BLAST, avec la matrice BLOSUM 62. On peut également utiliser les matrices PAM ou PAM250.
Le pourcentage d'identité entre deux séquences d'acides nucléiques ou d'acides aminés est déterminé en comparant ces deux séquences alignées de manière optimale dans laquelle la séquence d'acides nucléiques ou d'acides aminés à comparer peut comprendre des additions ou des délétions par rapport à la séquence de référence pour un alignement optimal entre ces deux séquences. Le pourcentage d'identité est calculé en déterminant le nombre de positions identiques pour lesquelles le nucleotide ou le résidu d'acide aminé est identique entre les deux séquences, en divisant ce nombre de positions identiques par le nombre total de positions comparées et en multipliant le résultat obtenu par 100 pour obtenir le pourcentage d'identité entre ces deux séquences.
Par séquences nucléiques présentant un pourcentage d'identité d'au moins 80 %, de préférence 85 % ou 90 %, de façon plus préférée 95 % voire 98 %, après alignement optimal avec une séquence de référence, on entend désigner les séquences nucléiques présentant, par rapport à la séquence nucléique de référence, certaines modifications comme en particulier une délétion, une troncation, un allongement, une fusion chimérique et/ou une substitution, notamment ponctuelle, et dont la séquence nucléique présente au moins 80 %, de préférence 85 %, 90 %, 95 % ou 98 %, d'identité après alignement optimal avec la séquence nucléique de référence. Il s'agit de préférence de séquences dont les séquences complémentaires sont susceptibles de s'hybrider spécifiquement avec les séquences de référence. De préférence, les conditions d'hybridation spécifiques ou de forte stringence seront telles qu'elles assurent au moins 80 %, de préférence 85 %, 90 %, 95 % ou 98 % d'identité après alignement optimal entre l'une des deux séquences et la séquence complémentaire de l'autre.
Une hybridation dans des conditions de forte stringence signifie que les conditions de température et de force ionique sont choisies de telle manière qu'elles permettent le maintien de l'hybridation entre deux fragments d'ADN complémentaires. A titre illustratif, des conditions de forte stringence de l'étape d'hybridation aux fins de définir les fragments poly nucléotidiques décrits ci-dessus, sont avantageusement les suivantes. L'hybridation ADN- ADN ou ADN-ARN est réalisée en deux étapes : (1) préhybridation à 42°C pendant 3 heures en tampon phosphate (20 mM, pH 7,5) contenant 5 x SSC (1 x SSC correspond à une solution 0,15 M NaCl + 0,015 M citrate de sodium), 50 % de formamide, 7 % de sodium dodécyl sulfate (SDS), 10 x Denhardt's, 5 % de dextran sulfate et 1 % d'ADN de sperme de saumon ; (2) hybridation proprement dite pendant 20 heures à une température dépendant de la taille de la sonde (i.e. : 42°C, pour une sonde de taille > 100 nucléotides) suivie de 2 lavages de 20 minutes à 20°C en 2 x SSC + 2 % SDS, 1 lavage de 20 minutes à 20°C en 0,1 x SSC + 0,1 % SDS. Le dernier lavage est pratiqué en 0,1 x SSC + 0,1 % SDS pendant 30 minutes à 60°C pour une sonde de taille > 100 nucléotides. Les conditions d'hybridation de forte stringence décrites ci-dessus pour un polynucléotide de taille définie, peuvent être adaptées par l'homme du métier pour des oligonucléotides de taille plus grande ou plus petite, selon l'enseignement de Sambrook et al., (1989, Molecular cloning : a laboratory manual. 2nd Ed. Cold Spring Harbor).
De plus, par fragment représentatif de séquences selon l'invention, on entend désigner tout fragment nucléotidique présentant au moins 15 nucléotides, de préférence au moins 20, 25, 30, 50, 75, 100, 150, 300 et 450 nucléotides consécutifs de la séquence dont il est issu. Par fragment représentatif, on entend en particulier une séquence nucléique codant pour un fragment biologiquement actif d'un polypeptide, tel que défini plus loin.
Par fragment représentatif, on entend également les séquences intergéniques, et en particulier les séquences nucléotidiques portant les signaux de régulation (promoteurs, terminateurs, voire enhancers ... ).
Parmi lesdits fragments représentatifs, on préfère ceux ayant des séquences nucléotidiques correspondant à des cadres ouverts de lecture, dénommés séquences ORFs (ORF pour « Open Reading Frame »), compris en général entre un codon d'initiation et un codon stop, ou entre deux codons stop, et codant pour des polypeptides, de préférence d'au moins 100 acides aminés, tel que par exemple, sans s'y limiter, les séquences ORFs qui seront décrites par la suite. La numérotation des séquences nucléotidiques ORFs qui sera utilisée par la suite dans la présente description correspond à la numérotation des séquences d'acides aminés des protéines codées par lesdites ORFs.
Les fragments représentatifs selon l'invention peuvent être obtenus par exemple par amplification spécifique telle que la PCR ou après digestion par des enzymes de restriction appropriés de séquences nucléotidiques selon l'invention, cette méthode étant décrite en particulier dans l'ouvrage de Sambrook et al.. Lesdits fragments représentatifs peuvent également être obtenus par synthèse chimique lors que leur taille n'est pas trop importante, selon des méthodes bien connues de l'homme du métier.
Parmi les séquences contenant des séquences de l'invention, ou des fragments représentatifs, on entend également les séquences qui sont naturellement encadrées par des séquences qui présentent au moins 80 %, 85 %, 90 %, 95 % ou 98 % d'identité avec les séquences selon l'invention. Par séquence nucléotidique modifiée, on entend toute séquence nucléotidique obtenue par mutagénèse selon des techniques bien connues de l'homme du métier, et comportant des modifications, de préférence au maximum 10 % de nucléotides modifiés, par rapport aux séquences normales, par exemple des mutations dans les séquences régulatrices et/ou promotrices de l'expression du polypeptide, notamment conduisant à une modification du taux d'expression ou de l'activité dudit polypeptide.
Par séquence nucléotidique modifiée, on entend également toute séquence nucléotidique codant pour un polypeptide modifié tel que définit ci-après.
Les fragments représentatifs selon l'invention peuvent également être des sondes ou amorces, qui peuvent être utilisées dans des procédés de détection, d'identification, de dosage ou d'amplification de séquences nucléiques.
Une sonde ou amorce se définit, au sens de l'invention, comme étant un fragment d'acides nucléiques simple brin ou un fragment double brin dénaturé comprenant par exemple de 12 bases à quelques kb, notamment de 15 à quelques centaines de bases, de préférence de 15 à 50 ou 100 bases, et possédant une spécificité d'hybridation dans des conditions déterminées pour former un complexe d'hybridation avec un acide nucléique cible. Les sondes et amorces selon l'invention peuvent être marquées directement ou indirectement par un composé radioactif ou non radioactif par des méthodes bien connues de l'homme du métier, afin d'obtenir un signal détectable et/ou quantifiable. Les séquences de polynucléotides selon l'invention non marquées peuvent être utilisées directement comme sonde ou amorce.
Les séquences sont généralement marquées pour obtenir des séquences utilisables pour de nombreuses applications. Le marquage des amorces ou des sondes selon l'invention est réalisé par des éléments radioactifs ou par des molécules non radioactives.
Parmi les isotopes radioactifs utilisés, on peut citer le P, le P, le S, le H ou le I25I. Les entités non radioactives sont sélectionnées parmi les ligands tels la biotine, l'avidine, la streptavidine, la dioxygénine, les haptènes, les colorants, les agents luminescents tels que les agents radioluminescents, chémoluminescents, bioluminescents, fluorescents, phosphorescents.
Les polynucléotides selon l'invention peuvent ainsi être utilisés comme amorce et/ou sonde dans des procédés mettant en oeuvre notamment la technique de PCR (amplification en chaîne par polymérase) (Rolfs et al., 1991, Berlin : Springer- Verlag). Cette technique nécessite le choix de paires d'amorces oligonucléotidiques encadrant le fragment qui doit être amplifié. On peut, par exemple, se référer à la technique décrite dans le brevet américain U.S. N° 4,683,202. Les fragments amplifiés peuvent être identifiés, par exemple après une •électrophorèse en gel d'agarose ou de polyacrylamide, ou après une technique chromatographique comme la filtration sur gel ou la chromatographie échangeuse d'ions, puis séquences. La spécificité de l'amplification peut être contrôlée en utilisant comme amorce les séquences nucléotidiques de polynucléotides de l'invention comme matrice, des plasmides contenant ces séquences ou encore les produits d'amplification dérivés. Les fragments nucléotidiques amplifiés peuvent être utilisés comme réactifs dans des réactions d'hybridation afin de mettre en évidence la présence, dans un échantillon biologique, d'un acide nucléique cible de séquence complémentaire à celle desdits fragments nucléotidiques amplifiés.
L'invention vise également les acides nucléiques susceptibles d'être obtenus par amplification à l'aide d'amorces selon l'invention. D'autres techniques d'amplification de l'acide nucléique cible peuvent être avantageusement employées comme alternative à la PCR (PCR-like) à l'aide de couple d'amorces de séquences nucléotidiques selon l'invention. Par PCR-like on entend désigner toutes les méthodes mettant en œuvre des reproductions directes ou indirectes des séquences d'acides nucléiques, ou bien dans lesquelles les systèmes de marquage ont été amplifiés, ces techniques sont bien entendu connues, en général il s'agit de l'amplification de l'ADN par une polymérase ; lorsque l'échantillon d'origine est un ARN il convient préalablement d'effectuer une transcription reverse. Il existe actuellement de très nombreux procédés permettant cette amplification, comme par exemple la technique SDA (Strand Displacement Amplification) ou technique d'amplification à déplacement de brin (Walker et al., 1992, Nucleic Acids Res. 20 : 1691), la technique TAS (Transcription-based Amplification System) décrite par Kwoh et al. (1989, Proc. Natl. Acad. Sci. USA, 86, 1173), la technique 3SR (Self-Sustained Séquence Replication) décrite par Guatelli et al. (1990, Proc. Natl. Acad. Sci. USA 87: 1874), la technique NASBA (Nucleic Acid Séquence Based Amplification) décrite par Kievitis et al. (1991, J. Virol. Methods, 35, 273), la technique TMA (Transcription Mediated Amplification), la technique LCR (Ligase Chain Reaction) décrite par Landegren et al. (1988, Science 241, 1077), la technique de RCR (Repair Chain Reaction) décrite par Segev (1992, Kessler C. Springer Veriag, Berlin, New- York, 197-205), la technique CPR (Cycling Probe Reaction) décrite par Duck et al. (1990, Biotechniques, 9, 142), la technique d'amplification à la Q-béta-réplicase décrite par Miele et al. (1983, J. Mol. Biol., 171, 281). Certaines de ces techniques ont depuis été perfectionnées. Dans le cas où le polynucléotide cible à détecter est un ARNm, on utilise avantageusement, préalablement à la mise en oeuvre d'une réaction d'amplification à l'aide des amorces selon l'invention ou à la mise en œuvre d'un procédé de détection à l'aide des sondes de l'invention, une enzyme de type transcriptase inverse afin d'obtenir un ADNc à partir de l'ARNm contenu dans l'échantillon biologique. L'ADNc obtenu servira alors de cible pour les amorces ou les sondes mises en oeuvre dans le procédé d'amplification ou de détection selon l'invention.
La technique d'hybridation de sondes peut être réalisée de manières diverses (Matthews et al., 1988, Anal. Biochem., 169, 1-25). La méthode la plus générale consiste à immobiliser l'acide nucléique extrait des cellules de différents tissus ou de cellules en culture sur un support (tels que la nitrocellulose, le nylon, le polystyrène) et à incuber, dans des conditions bien définies, l'acide nucléique cible immobilisé avec la sonde. Après l'hybridation, l'excès de sonde est éliminé et les molécules hybrides formées sont détectées par la méthode appropriée (mesure de la radioactivité, de la fluorescence ou de l'activité enzymatique liée à la sonde).
Selon un autre mode de mise en œuvre des sondes nucléiques selon l'invention, ces dernières peuvent être utilisées comme sondes de capture. Dans ce cas, une sonde, dite « sonde de capture », est immobilisée sur un support et sert à capturer par hybridation spécifique l'acide nucléique cible obtenu à partir de l'échantillon biologique à tester et l'acide nucléique cible est ensuite détecté grâce à une seconde sonde, dite « sonde de détection », marquée par un élément facilement détectable.
Parmi les fragments d'acides nucléiques intéressants, il faut ainsi citer en particulier les oligonucléotides anti-sens, c'est-à-dire dont la structure assure, par hybridation avec la séquence cible, une inhibition de l'expression du produit correspondant. Il faut également citer les oligonucléotides sens qui, par interaction avec des protéines impliquées dans la régulation de l'expression du produit correspondant, induiront soit une inhibition, soit une activation de cette expression. De façon préférée, les sondes ou amorces selon l'invention sont immobilisées sur un support, de manière covalente ou non covalente. En particulier, le support peut être une puce à ADN ou un filtre à haute densité, également objets de la présente invention.
On entend désigner par puce à ADN ou filtre haute densité, un support sur lequel sont fixées des séquences d'ADN, chacune d'entre elles pouvant être repérée par sa localisation géographique. Ces puces ou filtres diffèrent principalement par leur taille, le matériau du support, et éventuellement le nombre de séquences d'ADN qui y sont fixées.
On peut fixer les sondes ou amorces selon la première invention sur des supports solides, en particulier les puces à ADN, par différents procédés de fabrication. En particulier, on peut effectuer une synthèse in situ par adressage photochimique ou par jet d'encre. D'autres techniques consistent à effectuer une synthèse ex situ et à fixer les sondes sur le support de la puce à ADN par adressage mécanique, électronique ou par jet d'encre. Ces différents procédés sont bien connus de l'homme du métier.
Une séquence nucléotidique (sonde ou amorce) selon l'invention permet donc la détection et/ou l'amplification de séquences nucléiques spécifiques. En particulier, la détection de cesdites séquences est facilitée lorsque la sonde est fixée sur une puce à ADN, ou à un filtre haute densité.
L'utilisation de puces à ADN ou de filtres à haute densité permet en effet de déterminer l'expression de gènes dans un organisme présentant une séquence génomique proche de L. monocytogenes EGD-e. La séquence génomique de L. monocytogenes EGD-e, complétée par l'identification de tous les gènes de cet organisme, telle que présentée dans la présente invention, sert de base à la construction de ces puces à ADN ou filtre.
La préparation de ces filtres ou puces consiste à synthétiser des oligonucléotides, correspondant aux extrémités 5' et 3' des gènes. Ces oligonucléotides sont choisis en utilisant la séquence génomique et ses annotations divulguées par la présente invention. La température d'appariement des ces oligonucléotides aux places correspondantes sur l'ADN doit être approximativement la même pour chaque oligonucleotide. Ceci permet de préparer des fragments d'ADN correspondants à chaque gène par l'utilisation de condition de PCR appropriées dans un environnement hautement automatisée. Les fragments amplifiés sont ensuite immobilisés sur des filtres ou des supports en verre, silicium ou polymères synthétiques et ces milieux sont utilisés pour l'hybridation.
La disponibilité de tels filtres et/ou puces et de la séquence génomique correspondante annotée permet d'étudier l'expression de grands ensembles, voire de la totalité des gènes dans les micro-organismes associés à Listeria monocytogenes, en préparant les ADN complémentaires, et en les hybridant à l'ADN ou aux oligonucléotides immobilisés sur les filtres ou les puces. Egalement, les filtres et/ou les puces permettent d'étudier la variabilité des souches ou des espèces, en préparant l'ADN de ces organismes et en les hybridant à l'ADN ou aux oligonucléotides immobilisés sur les filtres ou les puces.
Les différences entre les séquences génomiques des différentes souches ou espèces peuvent grandement affecter l'intensité de l'hybridation et, par conséquent, perturber l'interprétation des résultats. Il peut donc être nécessaire d'avoir la séquence précise des gènes de la souche que l'on souhaite étudier. La méthode de détection des gènes décrite plus loin en détail, impliquant la détermination de la séquence de fragments aléatoires d'un génome, et les organisant d'après la séquence du génome complet de Listeria monocytogenes EGD-e divulgué dans la présente invention, peut être très utile.
Les séquences nucléotidiques selon l'invention peuvent être utilisées dans des puces à ADN pour effectuer l'analyse de mutations. Cette analyse repose sur la constitution de puces capables d'analyser chaque base d'une séquence nucléotidique selon l'invention. On pourra notamment à cette fin mettre en œuvre les techniques de micro-séquençage sur puce à ADN. Les mutations sont détectées par extension d'amorces immobilisées hybridant à la matrice des séquences analysées, juste en position adjacente de celle du nucleotide muté recherché. Une matrice simple-brin, ARN ou ADN, des séquences à analyser sera avantageusement préparée selon des méthodes classiques, à partir de produits amplifiés selon les techniques de type PCR. Les matrices d'ADN simple brin, ou d'ARN ainsi obtenues sont alors déposées sur la puce à ADN, dans des conditions permettant leur hybridation spécifique aux amorces immobilisées. Une polymérase thermostable, par exemple la Tth ou la Taq ADN polymérase, étend spécifiquement l'extrémité 3' de l'amorce immobilisée avec un analogue de nucleotide marqué complémentaire du nucleotide en position du site variable; par exemple un cycle thermique est réalisé en présence des didéoxyribonucléotides fluorescents. Les conditions expérimentales seront adaptées notamment aux puces employées, aux amorces immobilisées, aux polymérases employées, et au système de marquage choisi. Un avantage du microséquençage, par rapport aux techniques basées sur l'hybridation de sondes, est qu'il permet d'identifier tous les nucléotides variables avec une discrimination optimale dans des conditions de réactions homogènes; utilisé sur des puces à ADN, il permet une résolution et une spécificité optimales pour la détection routinière et industrielle de mutations en multiplex.
L'utilisation des filtres à haute densité et/ou des puces permet ainsi d'obtenir des connaissances nouvelles sur la régulation des gènes dans les organismes d'importance industrielle, et en particulier les listeria propagées dans diverses conditions. Elle permet aussi une identification rapide des différences entre les génomes des souches utilisées dans de multiples applications industrielles. En outre, une puce à ADN ou un filtre peut être un outil extrêmement intéressant pour la détermination, la détection et/ou l'identification d'un microorganisme. Ainsi, on préfère également les puces à ADN selon l'invention qui contiennent en outre au moins une séquence nucléotidique d'un microorganisme autre de Listeria monocytogenes, immobilisée sur le support de ladite puce. De préférence, le microorganisme choisi l'est parmi les bactéries du genre Listeria (ci- après désignées comme bactéries associées à L. monocytogenes), ou les variants de Listeria monocytogenes EGD-e.
Une puce à ADN ou un filtre selon l'invention est un élément très utile de certains kits ou nécessaires pour la détection et/ou l'identification de microorganismes, en particulier les bactéries appartenant à l'espèce Listeria monocytogenes ou les microorganismes associés, également objets de l'invention.
Par ailleurs, les puces à ADN ou les filtres selon l'invention, contenant des sondes ou amorces spécifiques de Listeria monocytogenes, sont des éléments très avantageux de kits ou nécessaires pour la détection et/ou la quantification de l'expression de gènes de Listeria monocytogenes (ou de microorganismes associés).
En effet, le contrôle de l'expression des gènes est un point critique pour optimiser la croissance et le rendement d'une souche, soit en permettant l'expression d'un ou plusieurs gènes nouveaux, soit en modifiant l'expression de gènes déjà présents dans la cellule. La présente invention fournit l'ensemble des séquences naturellement actives chez L. monocytogenes permettant l'expression des gènes. Elle permet ainsi la détermination de l'ensemble des séquences exprimées chez L. monocytogenes. Elle fournit également un outil permettant de repérer les gènes dont l'expression suit un schéma donné. Pour réaliser cela, l'ADN de tout ou partie des gènes de L. monocytogenes peut être amplifié grâce à des amorces selon l'invention, puis fixé à un support comme par exemple le verre ou le nylon ou une puce à ADN, afin de construire un outil permettant de suivre le profil d'expression de ces gènes. Cet outil, constitué de ce support contenant les séquences codantes sert de matrice d'hybridation à un mélange de molécules marquées reflétant les ARN messagers exprimés dans la cellule (en particulier les sondes marquées selon l'invention). En répétant cette expérience à différents instants et en combinant l'ensemble de ces données par un traitement approprié, on obtient alors les profils d'expression de l'ensemble de ces gènes. La connaissance des séquences qui suivent un schéma de régulation donnée peut aussi être mise à profit pour rechercher de manière dirigée, par exemple par homologie, d'autres séquences suivant globalement, mais de manière légèrement différente le même schéma de régulation. En complément, il est possible d'isoler chaque séquence de contrôle présente en amont des segments servant de sondes et d'en suivre l'activité à l'aide de moyen approprié comme un gène rapporteur (luciférase, β-galactosidase, GFP pour « Green Fluorescent Protein »). Ces séquences isolées peuvent ensuite être modifiées et assemblées par ingénierie métabolique avec des séquences d'intérêt en vue de leur expression optimale. Grâce à la séquence génomique présentée dans la présente invention, l'homme du métier saura identifier les gènes codant pour des protéines régulant la transcription des gènes de L. monocytogenes. Par ailleurs, le tableau I fournit la liste des phases ouvertes de lecture (ORF pour « Open Reading Frame) identifiées sur le génome de Listeria monocytogenes (SEQ ID N° 1), avec leur position sur ledit génome, et les fonctions putatives qui peuvent leur être attribuées. Toutefois, une telle liste ne doit pas être considérée comme limitative, une protéine pouvant faire avoir plusieurs rôles dans la cellule.
Modifier la structure ou l'intégrité de ces gènes pourra permettre de modifier l'expression des gènes cibles contrôlés par des promoteurs cibles de ces régulateurs. Ainsi, l'homme du métier pourra choisir le ou les régulateurs pertinents pour l'application recherchée ainsi que leur cible, ce qui permet l'optimisation de l'expression de gènes d'intérêt. L'utilisation des outils précédemment décrits tels les puces à ADN, permet aussi de repérer l'ensemble des gènes dont la régulation est modifiée par l'inactivation de certains gènes. Il est ainsi possible de sélectionner un ensemble de séquence de contrôle répondant, à des nuances près, à un même type de régulation. Ces séquences peuvent être alors utilisées pour contrôler l'expression de gènes d'intérêt.
L'invention concerne également les polypeptides codés par une séquence nucléotidique selon l'invention, de préférence, par un fragment représentatif de la séquence SEQ ID N° 1 et correspondant à une séquence ORF, telle que décrite dans le tableau L En particulier, les polypeptides de Listeria monocytogenes caractérisés en ce qu'ils sont choisis parmi les polypeptides de séquence SEQ ID N° 2 à SEQ ID N° 2854, de préférence de séquence SEQ ID N° 2 à SEQ ID N° 41 et SEQ ID N° 42 à SEQ ID N° 64 sont objet de l'invention.
L'invention comprend également les polypeptides caractérisés en ce qu'ils comprennent un polypeptide choisi parmi : a) un polypeptide selon l'invention ; b) un polypeptide présentant au moins 80 % de préférence 85 %, 90 %, 95 % et 98 % d'identité avec un polypeptide selon l'invention ; c) un fragment d'au moins 5 acides aminés d'un polypeptide selon l'invention, ou tel que défini en b) ; d) un fragment biologiquement actif d'un polypeptide selon l'invention, ou tel que défini en b) ou c) ; et e) un polypeptide modifié d'un polypeptide selon l'invention, ou tel que défini en b), c) ou d). Les séquences nucléotidiques codant pour les polypeptides décrits précédemment sont également objet de l'invention.
Dans la présente description, les termes polypeptides, séquences polypeptidiques, peptides et protéines sont interchangeables.
Il doit être compris que l'invention ne concerne pas les polypeptides sous forme naturelle, c'est-à-dire qu'ils ne sont pas pris dans leur environnement naturel mais qu'ils ont pu être isolés ou obtenus par purification à partir de sources naturelles, ou bien obtenus par recombinaison génétique, ou par synthèse chimique, et qu'ils peuvent alors comporter des acides aminés non naturels comme cela sera décrit plus loin.
Par polypeptide présentant un certain pourcentage d'identité avec un autre, que l'on désignera également par polypeptide homologue, on entend désigner les polypeptides présentant par rapport aux polypeptides naturels, certaines modifications, en particulier une délétion, addition ou substitution d'au moins un acide aminé, une troncation, un allongement, une solution chimérique et/ou une mutation, ou les polypeptides présentant des modifications post-traductionneîles. Parmi les polypeptides homologues, on préfère ceux dont la séquence d'acides aminés présentent au moins 80 %, de préférence 85 %, 90 %, 95 % et 98 % d'homologie avec les séquences d'acides aminés des polypeptides selon l'invention. Dans le cas d'une substitution, un ou plusieurs acide(s) aminé(s) consécutifs) ou non consécutif(s) sont remplacés par des acides aminés « équivalents ». L'expression « acides aminés équivalents » vise ici à désigner tout acide aminé susceptible d'être substitué à l'un des acides aminés de la structure de base sans cependant modifier essentiellement les activités biologiques des peptides correspondant et telles qu'elles seront définies par la suite.
Ces acides aminés équivalents peuvent être déterminés soit en s 'appuyant sur leur homologie de structure avec les acides aminés auxquels ils se substituent, soit sur des résultats d'essais comparatifs d'activité biologique entre les différents polypeptides susceptibles d'être effectués. A titre d'exemple, on mentionne les possibilités de substitution susceptibles d'être effectuées sans qu'il résulte en une modification approfondie de l'activité biologique du polypeptide modifié correspondant. On peut remplacer ainsi la leucine par la valine ou l'isoleucine, l'acide aspartique par l'acide glutamine, la glutamine par l'asparagine, l'arginine par la lysine, etc .. les substitutions inverses étant naturellement envisageables dans les mêmes conditions.
Les polypeptides homologues correspondent également aux polypeptides codés par les séquences nucléotidiques homologues ou identiques, telles que définies précédemment et comprennent ainsi dans la présente définition des polypeptides mutés ou correspondant à des variations inter ou intra espèces, pouvant exister chez Listeria, et qui correspondent notamment à des troncatures, substitutions, délétions et/ou additions, d'au moins un résidu d'acides aminés.
Il est entendu que l'on calcule le pourcentage d'identité entre deux polypeptides de la même façon qu'entre deux séquences d'acides nucléiques. Ainsi, le pourcentage d'identité entre deux polypeptides est calculé après alignement optimal de ces deux séquences, sur une fenêtre d'homologie maximale. Pour définir ladite fenêtre d'homologie maximale, on peut utiliser les mêmes algorithmes que pour les séquences d'acide nucléique.
Par fragment biologiquement actif d'un polypeptide selon l'invention, on entend désigner en particulier un fragment de polypeptide, tel que défini ci-après, présentant au moins une des caractéristiques biologiques des polypeptides selon l'invention, notamment en ce qu'il est capable d'exercer de manière générale une activité même partielle, tel que par exemple : - une activité enzymatique (métabolique) ou une activité pouvant être impliquée dans la biosynthèse ou la biodégradation de composés organiques ou inorganiques ;
- une activité structurelle (enveloppe cellulaire, molécule chaperonne, ribosome) ;
- une activité de transport (d'énergie, d'ion) ; ou dans la sécrétion de protéine ;
- une activité dans le processus de réplication, amplification, préparation, transcription, traduction ou maturation, notamment de l'ADN, de l' ARN ou des protéines.
Par fragment de polypeptides selon l'invention, on entend désigner un polypeptide comportant au minimum 5 acides aminés, de préférence 10, 15, 25, 50, 100 et 150 acides aminés.
Les fragments de polypeptides peuvent correspondre à des fragments isolés ou purifiés naturellement présents dans les souches de Listeria, ou à des fragments qui peuvent être obtenus par clivage dudit polypeptide par une enzyme protéolitique telle que la trypsine ou la chymotrypsine ou la collagénase, par un réactif chimique (bromure de cyanogène, CNBr) ou en plaçant ledit polypeptide dans un environnement très acide (par exemple à pH = 2,5). Des fragments polypeptidiques peuvent également être préparés par synthèse chimique, à partir d'hôtes transformés par un vecteur d'expression selon l'invention qui contiennent un acide nucléique permettant l'expression dudit fragment, et placé sous le contrôle des éléments de régulation et/ou d'expression appropriés.
Par « polypeptide modifié » d'un polypeptide selon l'invention, on entend désigner un polypeptide obtenu par recombinaison génétique ou par synthèse chimique comme décrit plus loin, qui présente au moins une modification par rapport à la séquence normale, de préférence au plus 10 % d'acides aminés modifiés par rapport à la séquence normale. Ces modifications peuvent être notamment portées sur des acides aminés nécessaires pour la spécificité ou l'efficacité de l'activité, ou à l'origine de la conformation structurale, de la charge, ou de l'hydrophobicité du polypeptide selon l'invention. On peut ainsi créer des polypeptides d'activité équivalente, augmentée ou diminuée, ou de spécificité équivalente, plus étroite ou plus large. Parmi les polypeptides modifiés, il faut citer les polypeptides dans lesquels jusqu'à cinq acides aminés peuvent être modifiés, tronqués à l'extrémité N ou C-terminale, ou bien délétés, ou ajoutés.
Comme cela est indiqué, les modifications d'un polypeptide ont pour objectif notamment : de permettre sa mise en œuvre dans des procédés de biosynthèse ou de biodégradation de composés organiques ou inorganiques,
- de permettre sa mise en œuvre dans des procédés de réplication, d'amplification, de réparation et règle de transcription, de traduction, ou de maturation notamment de l'ADN, TARN, ou de protéines, - de permettre sa sécrétion améliorée,
- de modifier sa solubilité, l'efficacité ou la spécificité de son activité, ou encore de faciliter sa purification.
La synthèse chimique présente également l'avantage de pouvoir utiliser des acides aminés non naturels ou des liaisons non peptidiques. Ainsi, il peut être intéressant d'utiliser des acides aminés non naturels, par exemple sous forme D, ou des analogues d'acides aminés, notamment des formes souffrées.
La présente invention fournit la séquence nucléotidique du génome de Listeria monocytogenes EGD-e, ainsi que certaines séquences polypeptidiques. L'homme du métier pourra déterminer les autres ORFs, en utilisant des méthodes connues, et des logiciels appropriés.
Parmi les gènes identifiés dans la séquence génomique de L. monocytogenes, on peut citer en particulier les gènes impliqués dans la biosynthèse de la vitamine B12 (SEQ ID N° 42 à SEQ ID N° 64). Cette bactérie est ainsi capable de synthétiser naturellement cette vitamine, et la connaissance des gènes menant à leur synthèse permet à l'homme du métier d'optimiser l'expression de ces gènes ou de les modifier en vue d'augmenter la production de cette vitamine. Ainsi, la présente invention a également pour objet un procédé de production de la vitamine B12, caractérisé en ce que l'on fournit le substrat de départ à une cellule hôte contenant les gènes correspondant à SEQ ID N° 42 à SEQ ID N° 64, que l'on la cultive dans les conditions appropriées pour la production de vitamine B12, et que l'on récupère ladite vitamine. De préférence, la cellule hôte est une cellule bactérienne, de façon plus préférée, une bactérie du genre Bacillus ou Listeria. Un procédé de production de la vitamine B12 utilisant une séquence nucléique, ou polypeptidique selon l'invention, ou une cellule hôte selon l'invention ou un animal ou un végétal selon l'invention est également un objet de la présente invention.
De manière générale, la liste des séquences SEQ ID, ou leur séquence
/ nucléique codante correspondante^ pourra être déterminée par l'homme de l'art à
5 parti des fonctions putatives les plus probables déterminées pour chacune des séquences SEQ ID dans le tableau 1 ci-après pour chacune des classes d'activité répertorié ci-après.
Ainsi et de manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide
10 de Listeria monocytogenes ou un de ses fragments impliqué dans la biosynthèse de la vitamine B12. De préférence, il s'agit d'un polypeptide de séquence SEQ ID N°
42 à SEQ ID N° 64.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide d'enveloppe
15 cellulaire ou présent à la surface de Listeria monocytogenes ou un de ses fragments.
De préférence, il s'agit d'un polypeptide de séquence SEQ ID N° 2 à SEQ ID N°
41.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention, caractérisées en ce qu'elle code pour un polypeptide de Listeria 20 monocytogenes ou un de ses fragments impliqué dans la biosynthèse des acides aminés.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans la biosynthèse des cofacteurs, 25 groupes prosthétiques et transporteurs.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans la machinerie cellulaire.
De manière préférée, l'invention est relative à une séquence nucléotidique 30 selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le métabolisme intermédiaire central. De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le métabolisme énergétique.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le métabolisme des acides gras et des phospholipides.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le métabolisme des nucléotides, des purines, des pyrimidines ou nucléosides.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans les fonctions de régulation. De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le processus de réplication.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le processus de transcription.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le processus de traduction.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le processus de transport et de liaison des protéines.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans l'adaptation aux conditions atypiques.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments dans la sensibilité aux médicaments et analogues.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans les fonctions relatives aux transposons.
De manière préférée, l'invention est relative à une séquence nucléotidique selon l'invention caractérisées en ce qu'elle code pour un polypeptide spécifique de
Listeria monocytogenes ou un de ses fragments. Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de
Listeria monocytogenes ou un de ses fragments impliqué dans la biosynthèse de la vitamine B12. De préférence, il s'agit d'un polypeptide de séquence SEQ ID N° 42 à SEQ ID N° 64. Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide d'enveloppe cellulaire ou de surface de Listeria monocytogenes ou un de ses fragments. De préférence, il s'agit d'un polypeptide de séquence SEQ ID N° 2 à
SEQ ID N° 41. Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de
Listeria monocytogenes ou un de ses fragments impliqué dans la bio synthèse des acides aminés.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de
Listeria monocytogenes ou un de ses fragments impliqué dans la biosynthèse des cofacteurs, groupes prosthétiques et transporteurs.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans la machinerie cellulaire.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le métabolisme intermédiaire central.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le métabolisme énergétique.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le métabolisme des acides gras et des phospholipides.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le métabolisme des nucléotides, des purines, des pyrimidines ou nucléosides. Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans les fonctions de régulation.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le processus de réplication.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le processus de transcription.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le processus de traduction.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans le processus de transport et de liaison des protéines.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans l'adaptation aux conditions atypiques.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments dans la sensibilité aux médicaments et analogues.
Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes ou un de ses fragments impliqué dans les fonctions relatives aux transposons. Sous un autre aspect, de manière préférée, l'invention a pour objet un polypeptide selon l'invention, caractérisé en ce qu'il s'agit d'un polypeptide spécifique de Listeria monocytogenes ou un de ses fragments.
Il est important de noter toutefois qu'un organisme vivant est un tout et doit être pris comme tel. Ainsi, afin de pouvoir se développer et d'exhiber ses propriétés, tout organisme a besoin d'interactions entre les différentes voies métaboliques.
Ainsi, la classification énoncée ci-dessus ne doit pas être considérée comme limitative, un gène pouvant être impliqué dans deux voies métaboliques distinctes.
La présente invention a également pour objet les séquences nucléotidiques et/ou de polypeptides selon l'invention, caractérisées en ce que lesdites séquences sont enregistrées sur un support d'enregistrement dont la forme et la nature facilitent la lecture, l'analyse et/ou l'exploitation de ladite ou desdites séquence(s). Ces supports peuvent également contenir d'autres informations extraites de la présente invention, notamment les analogies avec des séquences déjà connues, et/ou des informations concernant les séquences nucléotidiques et/ou de polypeptides d'autres microorganismes afin de faciliter l'analyse comparative et l'exploitation des résultats obtenus.
Parmi cesdits supports d'enregistrement, on préfère en particulier les supports lisibles par un ordinateur, tels les supports magnétiques, optiques, électriques ou hybrides, en particulier les disquettes informatiques, les CD-ROM, les serveurs informatiques. De tels supports d'enregistrement sont également objet de l'invention.
Les supports d'enregistrement selon l'invention, avec les informations apportées, sont très utiles pour le choix d'amorces ou de sondes nucléotidiques pour la détermination de gènes dans Listeria monocytogenes ou souches proches de cet organisme. De même, l'utilisation de ces supports pour l'étude du polymorphisme génétique de souche proche de Listeria monocytogenes, en particulier par la détermination des régions de colinéarité, est très utile dans la mesure où ces supports fournissent non seulement la séquence nucléotidique du génome de Listeria monocytogenes egb, mais également l'organisation génomique dans ladite séquence. Ainsi, les utilisations de supports d'enregistrement selon l'invention sont également des objets de l'invention.
L'analyse d'homologie entre différentes séquences s'effectue en effet avantageusement à l'aide de logiciels de comparaisons de séquences, tels le logiciel Blast, ou les logiciels de la trousse GCG, décrits précédemment.
L'invention vise également les vecteurs de clonage et/ou d'expression, qui contiennent une séquence nucléotidique selon l'invention. On préfère en particulier, les séquences nucléotidiques codant pour des polypeptides d'enveloppe cellulaire ou de surface, ou impliqués dans la machinerie cellulaire, en particulier la sécrétion, le métabolisme intermédiaire central, en particulier la production de sucre, le métabolisme énergétique, les processus de synthèse de la vitamine B12, de transcription et de traduction, de synthèse de polypeptides.
Les vecteurs selon l'invention comportent de préférence des éléments qui permettent l'expression et/ou la sécrétion des séquences nucléotidiques dans une cellule hôte déterminée.
Le vecteur doit alors comporter un promoteur, des signaux d'initiation et de terminaison de la traduction, ainsi que des régions appropriées de régulation de la transcription. Il doit pouvoir être maintenu de façon stable dans la cellule hôte et peut éventuellement posséder des signaux particuliers qui spécifient la sécrétion de la protéine traduite. Ces différents éléments sont choisis et optimisés par l'homme du métier en fonction de l'hôte cellulaire utilisé. A cet effet, les séquences nucléotidiques selon l'invention peuvent être insérées dans des vecteurs à réplication autonome au sein de l'hôte choisi, ou être des vecteurs intégratifs de l'hôte choisi.
De tels vecteurs sont préparés par des méthodes couramment utilisées par l'homme du métier, et les clones résultant peuvent être introduits dans un hôte approprié par des méthodes standards, telle que la lipofection, l'électroporation, le choc thermique, ou des méthodes chimiques.
Les vecteurs selon l'invention sont par exemple des vecteurs d'origine plasmidique ou virale. Ils sont utiles pour transformer des cellules hôtes afin de cloner ou d'exprimer les séquences nucléotidiques selon l'invention. L'invention comprend également les cellules hôtes transformées par un vecteur selon l'invention.
L'hôte cellulaire peut être choisi parmi des systèmes procaryotes ou eucaryotes, par exemple les cellules bactériennes mais également les cellules de levure ou les cellules animales, en particulier les cellules de mammifères. On peut également utiliser des cellules d'insectes ou des cellules de plantes. Les cellules hôtes préférées selon l'invention sont en particulier les cellules procaryotes, de préférence les bactéries appartenant au genre Listeria, à l'espèce Listeria monocytogenes, ou les microorganismes associés à l'espèce Listeria monocytogenes. L'invention concerne également les animaux, excepté l'homme, qui comprennent une cellule transformée selon l'invention. Les cellules transformées selon l'invention sont utilisables dans des procédés de préparation de polypeptides recombinants selon l'invention. Les procédés de préparation d'un polypeptide selon l'invention sous forme recombinante, caractérisés en ce qu'ils mettent en œuvre un vecteur et/ou une cellule transformée par un vecteur selon l'invention sont eux-mêmes compris dans la présente invention. De préférence, on cultive une cellule transformée par un vecteur selon l'invention dans des conditions qui permettent l'expression dudit polypeptide et on récupère ledit peptide recombinant. Les cellules hôtes selon l'invention peuvent également être utilisées pour la préparation de compositions alimentaires, qui sont elles-mêmes objet de la présente invention.
Ainsi qu'il a été dit, l'hôte cellulaire peut être choisi parmi des systèmes procaryotes ou eucaryotes. En particulier, il est possible d'identifier des séquences nucléotidiques selon l'invention, facilitant la sécrétion dans un tel système procaryote ou eucaryote. Un vecteur selon l'invention portant une telle séquence peut donc être avantageusement utilisé pour la production de protéines recombinantes, destinées à être sécrétées. En effet, la purification de ces protéines recombinantes d'intérêt sera facilité par le fait qu'elles sont présentent dans le surnageant de la culture cellulaire plutôt qu'à l'intérieur des cellules hôtes.
On peut également préparer les polypeptides selon l'invention par synthèse chimique. Un tel procédé de préparation est également un objet de l'invention. L'homme du métier connaît les procédés de synthèse chimique, par exemple les techniques mettant en œuvre des phases solides (voir notamment Steward et al., 1984, Solid phase peptides synthesis, Pierce Chem. Company, Rockford, 111, 2ème éd., (1984)) ou des techniques utilisant des phases solides partielles, par condensation de fragments ou par une synthèse en solution classique. Les polypeptides obtenus par synthèse chimique et pouvant comporter des acides aminés non naturels correspondant sont également compris dans l'invention. L'invention est en outre relative à des polypeptides hybrides présentant au moins un polypeptide ou un de ses fragments selon l'invention, et une séquence d'un polypeptide susceptible d'induire une réponse immunitaire chez l'homme ou l'animal.
Avantageusement, le déterminant antigénique est tel qu'il est susceptible d'induire une réponse humorale et/ou cellulaire.
Un tel déterminant pourra comprendre un polypeptide ou un de ses fragments selon l'invention sous forme glycosylée utilisé en vue d'obtenir des compositions immunogènes susceptibles d'induire la synthèse d'anticorps dirigés contre des épitopes multiples. Lesdits polypeptides ou leurs fragments glycosylés font également partie de l'invention.
Ces molécules hybrides peuvent être constituées en partie d'une molécule porteuse de polypeptides ou de leurs fragments selon l'invention, associée à une partie éventuellement immunogène, en particulier un épitope de la toxine diphtérique, la toxine tétanique, un antigène de surface du virus de l'hépatite B (brevet FR 79 21811), l'antigène VPl du virus de la poliomyélite ou toute autre toxine ou antigène viral ou bactérien.
Les procédés de synthèse des molécules hybrides englobent les méthodes utilisées en génie génétique pour construire des séquences nucléotidiques hybrides codant pour les séquences polypeptidiques recherchées. On pourra, par exemple, se référer avantageusement à la technique d'obtention de gènes codant pour des protéines de fusion décrite par Minton en 1984.
Lesdites séquences nucléotidiques hybrides codant pour un polypeptide hybride ainsi que les polypeptides hybrides selon l'invention caractérisés en ce qu'il s'agit de polypeptides recombinants obtenus par l'expression desdites séquences nucléotidiques hybrides, font également partie de l'invention.
L'invention comprend également les vecteurs caractérisés en ce qu'ils contiennent une desdites séquences nucléotidiques hybrides. Les cellules hôtes transformées par lesdits vecteurs, les animaux transgéniques comprenant une desdites cellules transformées ainsi que les procédés de préparation de polypeptides recombinants utilisant lesdits vecteurs, lesdites cellules transformées et/ou lesdits animaux transgéniques font bien entendu également partie de l'invention.
Le couplage entre un polypeptide selon l'invention et un polypeptide immunogène, peut être effectué par voie chimique, ou par voie biologique. Ainsi, selon l'invention, il est possible d'introduire un ou plusieurs élément(s) de liaison, notamment des acides aminés pour faciliter les réactions de couplage entre le polypeptide selon l'invention, et le polypeptide immunostimulateur, le couplage covalent de l'antigène immunostimulateur pouvant être réalisé à l'extrémité N ou C-terminale du polypeptide selon l'invention. Les réactifs bifonctionnels permettant ce couplage sont déterminés en fonction de l'extrémité choisie pour réaliser ce couplage, et les techniques de couplage sont bien connues de l'homme du métier.
Les conjugués issus d'un couplage de peptides peuvent être également préparés par recombinaison génétique. Le peptide hybride (conjugué) peut en effet être produit par des techniques d'ADN recombinant, par insertion ou addition à la séquence d'ADN codant pour le polypeptide selon l'invention, d'une séquence codant pour le ou les peptide(s) antigène(s), immunogène(s) ou haptène(s). Ces techniques de préparation de peptides hybrides par recombinaison génétique sont bien connues de l'homme du métier (voir par exemple Makrides, 1996, Microbiological Reviews 60, 512-538).
De préférence, ledit polypeptide immunitaire est choisi dans le groupe des peptides contenant les anatoxines, notamment le toxoïde diphtérique ou le toxoïde tétanique, les protéines dérivées du Streptocoque (comme la protéine de liaison à la séralbumine humaine), les protéines membranaires OMPA et les complexes de protéines de membranes externes, les vésicules de membranes externes ou les protéines de chocs thermiques.
Les polypeptides hybrides selon l'invention sont très utiles pour obtenir des anticorps monoclonaux ou polyclonaux, capables de reconnaître spécifiquement les polypeptides selon l'invention. En effet, un polypeptide hybride selon l'invention permet la potentiation de la réponse immunitaire, contre le polypeptide selon l'invention couplé à la molécule immunogène. De tels anticorps monoclonaux ou polyclonaux, leurs fragments, ou les anticorps chimériques, reconnaissant les polypeptides selon l'invention, sont également objets de l'invention.
Les anticorps monoclonaux spécifiques peuvent être obtenus selon la méthode classique de culture d'hybridome décrite par Kôhler et Mlstein (1975, Nature 256, 495).
Les anticorps selon l'invention sont par exemple des anticorps chimériques, des anticorps humanisés, des fragments Fab, ou F(ab')2. Il peut également se présenter sous forme d'immunoconjugué ou d'anticorps marqué afin d'obtenir un signal détectable et/ou quantifiable.
Ainsi, les anticorps selon l'invention peuvent être employés dans un procédé pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un microorganisme associé dans un échantillon biologique, caractérisé en ce qu'il comprend les étapes suivantes : a) mise en contact de l'échantillon biologique avec un anticorps selon l'invention ; b) mise en évidence du complexe antigène-anticorps éventuellement formé. Les anticorps selon la présente invention sont également utilisables afin de détecter une expression d'un gène de Listeria monocytogenes ou de microorganismes associés. En effet, la présence du produit d'expression d'un gène reconnu par un anticorps spécifique dudit produit expression peut être détectée par la présence d'un complexe antigène-anticorps formé après la mise en contact de la souche de Listeria monocytogenes ou du microorganisme associé avec un anticorps selon l'invention. La souche bactérienne utilisée peut avoir été « préparée », c'est-à- dire centrifugée, lysée, placée dans un réactif approprié pour la constitution du milieu propice à la réaction immunologique. En particulier, on préfère un procédé de détection de l'expression dans le gène, correspondant à un Western blot, pouvant être effectué après une électrophorèse sur gel de polyacrylamide d'un lysat de la souche bactérienne, en présence ou en l'absence de conditions réductrices (SDS- PAGE). Après migration et séparation des protéines sur le gel de polyacrylamide, on transfère lesdites protéines sur une membrane appropriée (par exemple en nylon) et on détecte la présence de la protéine ou du polypeptide d'intérêt, par mise en contact de ladite membrane avec un anticorps selon l'invention.
Ainsi, la présente invention comprend également les kits ou nécessaires pour la mise en œuvre d'un procédé tel que décrit (de détection de l'expression d'un gène de Listeria monocytogenes ou d'un microorganisme associé, ou pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou un microorganisme associé), comprenant les éléments suivants : a) un anticorps polyclonal ou monoclonal selon l'invention ; b) éventuellement, les réactifs pour la constitution du milieu propice à la réaction immunologique ; c) éventuellement, les réactifs permettant la mise en évidence des complexes antigène-anticorps produits par la réaction immunologique.
Les polypeptides et les anticorps selon l'invention peuvent avantageusement être immobilisés sur un support, notamment une puce à protéines. Une telle puce à protéines est un objet de l'invention, et peut également contenir au moins un polypeptide d'un microorganisme autre que Listeria monocytogenes ou un anticorps dirigé contre un composé d'un microorganisme autre que Listeria monocytogenes.
Les puces à protéines ou filtres à haute densité contenant des protéines selon l'invention peuvent être construits de la même manière que les puces à ADN selon l'invention. En pratique, on peut effectuer la synthèse des polypeptides fixés directement sur la puce à protéines, ou effectuer une synthèse ex situ suivie d'une étape de fixation du polypeptide synthétisé sur ladite puce. Cette dernière méthode est préférable, lorsque l'on désire fixer des protéines de taille importante sur le support, qui sont avantageusement préparées par génie génétique. Toutefois, si l'on ne désire fixer que des peptides sur le support de ladite puce, il peut être plus intéressant de procéder à la synthèse desdits peptides directement in situ.
Les puces à protéines selon l'invention peuvent être avantageusement utilisées dans des kits ou nécessaires pour la détection et/ou l'identification de bactéries associées à l'espèce Listeria monocytogenes ou à un microorganisme, ou de façon plus générale dans des kits ou nécessaires pour la détection et/ou l'identification de microorganismes. Lorsque l'on fixe les polypeptides selon l'invention sur les puces à ADN, on recherche la présence d'anticorps dans les échantillons testés, la fixation d'un anticorps selon l'invention sur le support de la puce à protéines permettant l'identification de la protéine dont ledit anticorps est spécifique.
De préférence, on fixe un anticorps selon l'invention sur le support de la puce à protéines, et on détecte la présence de l'antigène correspondant, spécifique de Listeria monocytogenes ou d'un microorganisme associé.
Une puce à protéines ci-dessus décrite peut être utilisée pour la détection de produits de gènes, pour établir un profil d'expression desdits gènes, en complément d'une puce à ADN selon l'invention.
Les puces à protéines selon l'invention sont également extrêmement utiles pour les expériences de protéomique, qui étudie les interactions entre les différentes protéines d'un microorganisme donné. De façon simplifiée, on fixe des peptides représentatifs des différentes protéines d'un organisme sur un support. Puis, on met ledit support en contact avec des protéines marquées, et après une étape optionnelle de rinçage, on détecte des interactions entre lesdites protéines marquées et les peptides fixés sur la puce à protéines.
Ainsi, les puces à protéines comprenant une séquence polypeptidique selon l'invention ou un anticorps selon l'invention sont objet de l'invention, ainsi que les kits ou nécessaires les contenant.
La présente invention couvre également un procédé de détection et/ou d'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un microorganisme associé dans un échantillon biologique, qui met en œuvre une séquence nucléotidique selon l'invention.
Il doit être entendu que le terme échantillon biologique concerne dans la présente invention les échantillons prélevés à partir d'un organisme vivant (en particulier sang, tissus, organes ou autres prélevés à partir d'un mammifère) ou un échantillon contenant du matériel biologique, c'est-à-dire de l'ADN. Un tel échantillon biologique englobe donc les compositions alimentaires contenant des bactéries (par exemple les fromages, les produits laitiers), mais également des compositions alimentaires contenant des levures (bières, pains) ou autres.
Le procédé de détection et/ou d'identification mettant en œuvre les séquences nucléotidiques selon l'invention peut être de diverse nature.
On préfère un procédé comportant les étapes suivantes : a) éventuellement, isolement de l'ADN à partir de l'échantillon biologique à analyser, ou obtention d'un ADNc à partir de l'ARN de l'échantillon biologique ; b) amplification spécifique de l'ADN de bactéries appartenant à l'espèce
Listeria monocytogenes ou à un micro-organisme associé à l'aide d'au moins une amorce selon l'invention ; c) mise en évidence des produits d'amplification.
Ce procédé est basé sur l'amplification spécifique de l'ADN, en particulier par une réaction d'amplification en chaîne.
On préfère également un procédé comprenant les étapes suivantes : a) mise en contact d'une sonde nucléotidique selon l'invention avec un échantillon biologique, l'acide nucléique contenu dans l'échantillon biologique ayant, le cas échéant, préalablement été rendu accessible à l'hybridation, dans des conditions permettant l'hybridation de la sonde à l'acide nucléique d'une bactérie appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé ; b) mise en évidence de l'hybride éventuellement formé entre la sonde nucléotidique et l'ADN de l'échantillon biologique. Un tel procédé ne doit pas être limité à la détection de la présence de l'ADN contenu dans l'échantillon biologique attesté, il peut être également mis en œuvre pour détecter l'ARN contenu dans ledit échantillon. Ce procédé englobe en particulier les Southern et Northern blot.
Un autre procédé préféré selon l'invention comprend les étapes suivantes : a) mise en contact d'une sonde nucléotidique immobilisée sur un support selon l'invention avec un échantillon biologique, l'acide nucléique de l'échantillon, ayant, le cas échéant, été préalablement rendu accessible à l'hybridation, dans des conditions permettant l'hybridation de la sonde à l'acide nucléique d'une bactérie appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé ; b) mise en contact de l'hybride formé entre la sonde nucléotidique immobilisée sur un support et l'acide nucléique contenu dans l'échantillon biologique, le cas échéant après élimination de l'ADN de l'échantillon biologique n'ayant pas hybride avec la sonde, avec une sonde nucléotidique marquée selon l'invention ; c) mise en évidence du nouvel hybride formé à l'étape b).
Ce procédé est avantageusement utilisé avec une puce à ADN selon l'invention, l'acide nucléique recherché s'hybridant avec une sonde présente à la surface de ladite puce, et étant détecté par l'utilisation d'une sonde marquée. Ce procédé est avantageusement mis en œuvre en combinant une étape préalable d'amplification de l'ADN ou de l'ADN complémentaire obtenu éventuellement par transcription inverse, à l'aide d'amorces selon l'invention. Ainsi, la présente invention englobe également les kits ou nécessaires pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé, caractérisé en ce qu'il comprend les éléments suivants : a) une sonde nucléotidique selon l'invention ; b) éventuellement, les réactifs nécessaires à la mise en œuvre d'une réaction d'hybridation ; c) éventuellement, au moins une amorce selon l'invention ainsi que les réactifs nécessaires à une réaction d'amplification de l'ADN. De même, la présente invention englobe également les kits ou nécessaires pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé, caractérisé en ce qu'il comprend les éléments suivants : a) une sonde nucléotidique, dite sonde de capture, selon l'invention; b) une sonde oligonucléotidique, dite sonde de révélation, selon l'invention ; c) éventuellement, au moins une amorce selon l'invention ainsi que les réactifs nécessaires à une réaction d'amplification de l'ADN. Enfin, les kits ou nécessaires pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé, caractérisé en ce qu'il comprend les éléments suivants : a) au moins une amorce selon l'invention ; b) éventuellement, les réactifs nécessaires pour effectuer une réaction d'amplification d'ADN ; c) éventuellement, un composant permettant de vérifier la séquence du fragment amplifié, plus particulièrement une sonde oligonucléotidique selon l'invention. sont également objets de la présente invention.
De préférence, lesdites amorces et/ou sondes et/ou polypeptides et/ou anticorps selon la présente invention utilisés dans les procédés et/ou kits ou nécessaires selon la présente invention sont choisis parmi les amorces et/ou sondes et/ou polypeptides et/ou anticorps spécifiques de l'espèce Listeria monocytogenes. De manière préférée, ces éléments sont choisis parmi les séquences nucléotidiques condant pour une protéine sécrétée, parmi les polypeptides sécrétés, ou parmi les anticorps dirigés contre des polypeptides sécrétés de Listeria monocytogenes.
La présente invention a également pour objet les souches de Listeria monocytogenes et/ou de microorganismes associés contenant une ou plusieurs mutation(s) dans une séquence nucléotidique selon l'invention, en particulier une séquence ORF, ou leurs éléments régulateurs (en particulier promoteurs).
On préfère, selon la présente invention, les souches de Listeria monocytogenes présentant une ou plusieurs mutation(s) dans les séquences nucléotidiques codant pour des polypeptides impliqués dans la machine cellulaire, en particulier la sécrétion, le métabolisme intermédiaire central, le métabolisme énergétique, les processus de synthèse des acides aminés, de transcription et de traduction, de synthèse des polypeptides.
Lesdites mutations peuvent mener à une inactivation du gène, ou en particulier lorsqu'elles sont situées dans les éléments régulateurs dudit gène, à une surexpression de celui-ci.
L'invention concerne en outre l'utilisation d'une séquence nucléotidique selon l'invention, d'un polypeptide selon l'invention, d'un anticorps selon l'invention, d'une cellule selon l'invention, et/ou d'un animal transformé selon l'invention, pour la sélection de composé organique ou inorganique capable de moduler, de réguler, d'induire ou d'inhiber l'expression de gènes, et/ou de modifier la réplication cellulaire de cellules eucaryotes ou procaryotes ou capables d'induire, d'inhiber ou d'aggraver les pathologies liées à une infection par Listeria monocytogenes ou un de ses micro-organismes associés.
L'invention comprend également une méthode de sélection de composés capables de se lier à un polypeptide ou un de ses fragments selon l'invention, capables de se lier à une séquence nucléotidique selon l'invention, ou capable de reconnaître un anticorps selon la revendication, et/ou capables de moduler, de réguler, d'induire ou d'inhiber l'expression de gènes, et/ou de modifier la croissance ou la réplication cellulaire de cellules eucaryotes ou procaryotes, ou capables d'induire, d'inhiber ou d'aggraver chez un organisme animal ou humain les pathologies liées à une infection par Listeria monocytogenes, ou un de ses micro-organismes associés, caractérisée en ce qu'elle comprend les étapes suivantes : a) mise en contact dudit composé avec ledit polypeptide, ladite séquence nucléotidique, avec une cellule transformée selon l'invention et/ou administration dudit composé à un animal transformé selon l'invention; b) détermination de la capacité dudit composé à se lier avec ledit polypeptide ou ladite séquence nucléotidique, ou de moduler, de réguler, d'induire ou d'inhiber l'expression de gènes, ou de moduler la croissance ou la réplication cellulaire, ou d'induire, d'inhiber ou d'aggraver chez ledit animal transformé les pathologies liées à une infection par Listeria monocytogenes ou un de ses micro-organismes associés.
Les cellules et/ou les animaux transformés selon l'invention, pourront avantageusement servir de modèle et être utilisés dans des procédés pour étudier, identifier et/ou sélectionner des composés susceptibles d'être responsables de pathologies induites ou aggravées par Listeria monocytogenes, ou susceptibles de prévenir et/ou de traiter ces pathologies comme par exemple les maladies génitales, oculaires ou systémiques, notamment du système lymphatique. En particulier, les cellules hôtes transformées, notamment les bactéries de la famille des Listeriae dont la transformation par un vecteur selon l'invention peut par exemple accroître ou inhiber son pouvoir infectieux, ou moduler les pathologies habituellement induites ou aggravées par l'infection, pourront être utilisées pour infecter des animaux dont on suivra l'apparition des pathologies. Ces animaux non transformés, infectés par exemple avec des bactéries Listeriae transformées, pourront servir de modèle d'étude. De la même manière, les animaux transformés selon l'invention pourront être utilisés dans des procédés de sélection de composés susceptibles de prévenir et/ou de traiter les maladies dues à Listeria. Lesdits procédés utilisant lesdites cellules transformées et/ou animaux transformés, font partie de l'invention.
Les composés susceptibles d'être sélectionnés peuvent être des composés organiques tels que des polypeptides ou hydrates de carbone ou tous autres composés organiques ou inorganiques déjà connus, ou des composés organiques nouveaux élaborés à partir de techniques de modélisation moléculaire et obtenus par synthèse chimique ou biochimique, ces techniques étant connues de l'homme de l'art. Lesdits composés sélectionnés pourront être utilisés pour moduler la croissance et/ou la réplication cellulaire de Listeria monocytogenes ou tout autre micro-organisme associé et ainsi pour contrôler l'infection par ces microorganismes. Lesdits composés selon l'invention pourront également être utilisés pour moduler la croissance et/ou la réplication cellulaire de toutes cellules eucaryotes ou procaryotes, notamment les cellules tumorales et les microorganismes infectieux, pour lesquelles lesdits composés s'avéreront actifs, les méthodes permettant de déterminer lesdites modulations étant bien connues de l'homme de l'art.
On entend désigner par composé capable de moduler la croissance d'un micro-organisme tout composé permettant de d'intervenir, de modifier, de limiter et/ou de réduire le développement, la croissance, la vitesse de prolifération et/ou la viabilité dudit micro-organisme.
Cette modulation peut être réalisée par exemple par un agent capable de se lier à une protéine et ainsi d'inhiber ou de potentialiser son activité biologique, ou capable de se lier à une protéine membranaire de la surface extérieure d'un microorganisme et de bloquer la pénétration dudit micro-organisme dans la cellule hôte ou de favoriser l'action du système immunitaire de l'organisme infecté dirigé à l'encontre dudit micro-organisme. Cette modulation peut être également réalisée par un agent capable de se lier à une séquence nucléotidique d'un ADN ou ARN d'un micro-organisme et de bloquer par exemple l'expression d'un polypeptide dont l'activité biologique ou structurelle est nécessaire à la croissance ou à la reproduction dudit micro-organisme. On entend désigner par micro-organisme associé dans la présente invention, tout micro-organisme dont l'expression de gène peut être modulée, régulée, induite ou inhibée, ou dont la croissance ou la réplication cellulaire peut être également modulée par un composé de l'invention. On entend désigner également par microorganisme associé dans la présente invention, tout micro-organisme comportant des séquences nucléotidiques ou des polypeptides selon l'invention. Ces microorganismes peuvent dans certains cas comporter des polypeptides ou des séquences nucléotidiques identiques ou homologues à celles de l'invention pourront également être détectés et/ou identifiés par les procédés ou kit de détection et/ou d'identification selon l'invention et également servir de cible pour les composés de l'invention.
L'invention concerne les composés susceptibles d'être sélectionnés par une méthode de sélection selon l'invention.
L'invention concerne également une composition pharmaceutique comprenant un composé choisi parmi les composés suivants : a) une séquence nucléotidique selon l'invention ; b) un polypeptide selon l'invention ; c) un vecteur selon l'invention ; d) un anticorps selon l'invention ; et e) un composé susceptible d'être sélectionné par une méthode de sélection selon l'invention, éventuellement en association avec un véhicule pharmaceutiquement acceptable. On entend désigner par quantité efficace, une quantité suffisante dudit composé ou anticorps, ou de polypeptide de l'invention, permettant de moduler la croissance de Listeria monocytogenes ou d'un micro-organisme associé. L'invention concerne aussi une composition pharmaceutique selon l'invention pour la prévention ou le traitement d'une infection par une bactérie appartenant à l'espèce Listeria monocytogenes ou par un micro-organisme associé. L'invention vise en outre une composition immunogène et/ou vaccinale, caractérisée en ce qu'elle comprend un ou plusieurs polypeptides selon l'invention et/ou un ou plusieurs polypeptides hybrides selon l'invention.
L'invention comprend aussi l'utilisation d'une cellule transformée selon l'invention, pour la préparation d'une composition vaccinale.
L'invention vise également une composition vaccinale, caractérisée en ce qu'elle contient une séquence nucléotidique selon l'invention, un vecteur selon l'invention et/ou une cellule transformée selon l'invention.
L'invention concerne également les compositions vaccinales selon l'invention, pour la prévention ou le traitement d'une infection par une bactérie appartenant à l'espèce Listeria monocytogenes ou par un micro-organisme associé.
De manière préférée, les compositions immunogènes et/ou vaccinales selon l'invention destinées à la prévention et/ou au traitement d'infection par Listeria monocytogenes ou par un micro-organisme associé seront choisies parmi les compositions immunogènes et/ou vaccinales comprenant un polypeptide ou un de ses fragments correspondant à une protéine, ou un de ses fragments, de l'enveloppe cellulaire de Listeria monocytogenes. Les compositions vaccinales comprenant des séquences nucléotidiques comprendront de préférence également des séquences nucléotidiques codant pour un polypeptide ou un de ses fragments correspondant à une protéine, ou un de ses fragments, de l'enveloppe cellulaire de Listeria monocytogenes.
Parmi ces compositions immunogènes et/ou vaccinales préférées, les plus préférées sont celles comprenant un polypeptide ou un de ses fragments, ou une séquence nucléotidique ou un de ses fragments dont les séquences sont choisies parmi les séquences nucléotidiques ou d'acides aminés identifiées dans ce groupe fonctionnel et listées précédemment.
Les polypeptides de l'invention ou leurs fragments entrant dans les compositions immunogènes selon l'invention peuvent être sélectionnés par des techniques connues de l'homme de l'art comme par exemple sur la capacité desdits polypeptides à stimuler les cellules T, qui se traduit par exemple par leur prolifération ou la sécrétion d'interleukines, et qui aboutit à la production d'anticorps dirigés contre lesdits polypeptides. Chez la souris, chez laquelle une dose pondérale de la composition vaccinale comparable à la dose utilisée chez l'homme est administrée, la réaction anticorps est testée par prélèvement du sérum suivi d'une étude de la formation d'un complexe entre les anticorps présents dans le sérum et l'antigène de la composition vaccinale, selon les techniques usuelles.
Selon l'invention, lesdites compositions vaccinales seront de préférence en association avec un véhicule pharmaceutiquement acceptable et, le cas échéant, avec un ou plusieurs adjuvants de l'immunité appropriés.
Aujourd'hui, divers types de vaccins sont disponibles pour protéger l'homme contre des maladies infectieuses : micro-organismes vivants atténués (M. bovis - BCG pour la tuberculose), micro-organismes inactivés (virus de la grippe), des extraits acellulaires (Bordetella pertussis pour la coqueluche), protéines recombinées (antigène de surface du virus de l'hépatite B), des polyosides (pneumocoques). Des vaccins préparés à partir de peptides de synthèse ou de micro- organismes génétiquement modifiés exprimant des antigènes hétérologues sont en cours d'expérimentation. Plus récemment encore, des ADNs plasmidiques recombinés portant des gènes codant pour des antigènes protecteurs ont été proposés comme stratégie vaccinale alternative. Ce type de vaccination est réalisé avec un plasmide particulier dérivant d'un plasmide de E. coli qui ne se réplique pas in vivo et qui code uniquement pour la protéine vaccinante. Des animaux ont été immunisés en injectant simplement l'ADN plasmidique nu dans le muscle. Cette technique conduit à l'expression de la protéine vaccinale in situ et à une réponse immunitaire de type cellulaire (CTL) et de type humoral (anticorps). Cette double induction de la réponse immunitaire est l'un des principaux avantages de la technique de vaccination avec de l'ADN nu.
Les compositions vaccinales comprenant des séquences nucléotidiques ou des vecteurs dans lesquels sont insérées lesdites séquences, sont notamment décrites dans la demande internationale N° WO 90/11092 et également dans la demande internationale N° WO 95/11307. La séquence nucléotidique constitutive de la composition vaccinale selon l'invention peut être injectée à l'hôte après avoir été couplée à des composés qui favorisent la pénétration de ce polynucléotide à l'intérieur de la cellule ou son transport jusqu'au noyau cellulaire. Les conjugués résultants peuvent être encapsulés dans des microparticules polymères, comme décrit dans la demande internationale N° WO 94/27238 (Medisorb Technologies International).
Selon un autre mode de réalisation de la composition vaccinale selon l'invention, la séquence nucléotidique, de préférence un ADN, est complexée avec du DEAE-dextran, avec des protéines nucléaires, avec des lipides ou encapsulée dans des liposomes ou encore introduite sous la forme d'un gel facilitant sa transfection dans les cellules. Le polynucléotide ou le vecteur selon l'invention peut aussi être en suspension dans une solution tampon ou être associé à des liposomes. Avantageusement, un tel vaccin sera préparé conformément à la technique décrite par Tacson et al. ou Huygen et al. en 1996 ou encore conformément à la technique décrite par Davis et al. dans la demande internationale N° WO 95/11307.
Un tel vaccin peut être également préparé sous la forme d'une composition contenant un vecteur selon l'invention, placée sous le contrôle d'éléments de régulation permettant son expression chez l'homme ou l'animal. On pourra par exemple utiliser, en tant que vecteur d'expression in vivo de l'antigène polypeptidique d'intérêt, le plasmide pcDNA3 ou le plasmide pcDNAl/neo, tous les deux commercialisés par Invitrogen (R & D Systems, Abingdon, Royaume-Uni). Un tel vaccin comprendra avantageusement, outre le vecteur recombinant, une solution saline, par exemple une solution de chlorure de sodium. On entend désigner par véhicule pharmaceutiquement acceptable, un composé ou une combinaison de composés entrant dans une composition pharmaceutique ou vaccinale ne provoquant pas de réactions secondaires et qui permet par exemple la facilitation de l'administration du composé actif, l'augmentation de sa durée de vie et/ou de son efficacité dans l'organisme, l'augmentation de sa solubilité en solution ou encore l'amélioration de sa conservation. Ces véhicules pharmaceutiquement acceptables sont bien connus et seront adaptés par l'homme de l'art en fonction de la nature et du mode d'administration du composé actif choisi.
En ce qui concerne les formulations vaccinales, celles-ci peuvent comprendre des adjuvants de l'immunité appropriés qui sont connus de l'homme de l'art, comme par exemple l'hydroxyde d'aluminium, un représentant de la famille des muramyl peptides comme un des dérivés peptidiques du N-acétyl-muramyl, un lysat bactérien, ou encore l'adjuvant incomplet de Freund. De préférence, ces composés seront administrés par voie systémique, en particulier par voie intraveineuse, par voie intramusculaire, intradermique ou sous- cutanée, ou par voie orale. De manière plus préférée, la composition vaccinale comprenant des polypeptides selon l'invention, sera administrée à plusieurs reprises, de manière étalée dans le temps, par voie intradermique ou sous-cutanée.
Leurs modes d'administration, posologies et formes galéniques optimaux peuvent être déterminés selon les critères généralement pris en compte dans l'établissement d'un traitement adapté à un patient comme par exemple l'âge ou le poids corporel du patient, la gravité de son état général, la tolérance au traitement et les effets secondaires constatés.
L'invention comprend l'utilisation d'une composition selon l'invention, pour le traitement ou la prévention de maladies génitales, induites ou aggravées par Listeria monocytogenes.
Enfin, l'invention comprend l'utilisation d'une composition selon l'invention, pour le traitement ou la prévention de maladiesinduites ou aggravées par la présence de Listeria monocytogenes.
Enfin, l'invention comprend l'utilisation d'une composition selon l'invention, pour le traitement ou la prévention de maladies systémiques, notamment du système lymphatique, induites ou aggravées par la présence de Listeria monocytogenes.
Par ailleurs, la présente invention a également pour objet une banque d'ADN génomique d'une bactérie du genre Listeria, de manière préférée, Listeria monocytogenes, de manière préférée la souche EGD-e, ladite banque d'ADN étant clonée dans des chromosomes artificiels de bactéries (BAC). Une telle banque d'ADN génomique contient de très larges inserts du génome de Listeria, en particulier des inserts d'une taille comprise entre 50 et 200 kb.
Un des avantages de l'utilisation du système de BAC par rapport à un système de cosmides est que le plasmide utilisé n'est présent qu'à une ou au maximum deux copies par cellule transformée, ce qui réduit le potentiel pour la recombinaison entre des fragments d'ADN, et de façon plus importante, ce qui élimine le risque de surexpression létale de gènes clones bactériens. Néanmoins, la présence du BAC en tant que simple copie signifie que l' ADN plasmidique doit être extrait d'un large volume de culture afin d'obtenir suffisamment d'ADN pour la séquence. De plus, la stabilité et la fidélité de maintenance des clones dans une banque de BAC permettent l'identification de différences génomiques parmi différentes souches de listeria, et l'identification de ces différences génétiques qui peuvent être responsables des variations phénotypiques observées entre les différentes souches.
La banque d'ADN génomique décrite dans la présente invention, en particulier la banque LM_baclim déposée à la CNCM le 11 avril 2000 sous le numéro d'ordre n°I-2439 recouvre en effet le génome de Listeria monocytogenes. Toutefois, bien que certaines régions n'aient pas pu être clonées dans ladite banque, en raison de problèmes de létalités chez Escherichia coli, ces régions peuvent facilement être amplifiées et identifiées par l'homme du métier, en utilisant des oligonucléotides spécifiques des séquences des extrémités des différents clones qui forment les contigs.
La présente invention concerne également les méthodes pour l'isolement d'un polynucléotide d'intérêt présent chez une souche de Listeria et absente chez une autre souche, qui utilise au moins une banque d'ADN basée sur un BAC, contenant le génome de Listeria. La méthode selon l'invention pour l'isolement d'un polynucléotide d'intérêt peut comprendre les étapes suivantes : a) isoler au moins un polynucléotide contenu dans un clone de la banque d'ADN basée sur un BAC, d'origine de listeria, b) isoler :
- au moins un polynucléotide génomique ou ADNc d'une listeria, ladite listeria appartenant à une souche différente de la souche utilisée pour la construction de la banque d'ADN BAC de l'étape a) ou, de façon alternative, au moins un polynucléotide contenu dans un clone d'une banque d'ADN basée sur un BAC préparé à partir du génome d'une listeria qui est différente de la listeria utilisée pour la construction de la banque d'ADN basée sur le BAC de l'étape a). c) hybrider le polynucléotide de l'étape a) au polynucléotide de l'étape b) ; d) sélectionner les polynucléotides de l'étape a) qui n'ont pas formé de complexe d'hybridation avec les polynucléotides de l'étape b) ; e) caractériser le polynucléotide sélectionné. On peut préparer le polynucléotide de l'étape a) par la digestion d'au moins un clone recombinant BAC avec une enzyme de restriction appropriée, et de façon optionnelle, l'amplification de Pinsert polynucléotide qui en résulte.
Ainsi, la méthode de l'invention permet à l'homme du métier d'effectuer des études génomiques comparatives entre les différentes souches ou espèces du genre listeria, par exemple entre les souches pathogéniques et leurs équivalant non pathogènes.
En particulier, il est possible d'étudier et de déterminer les régions de polymorphisme entre lesdites souches.
EXEMPLES
Exemple 1 : Fabrication de la banque BAC d'ADN de Listeria monocytogenes
Les blocs contenant l'ADN chromosomique de Listeria monocytogenes, d'un poids moyen de 80 mg ont été préparés dans l'agarose, par des méthodes connues de l'homme du métier. Ils ont été gardés dans une solution de 500 mM EDTA, pH8,0.
On utilise 8 blocs pour la construction de la banque. Ces blocs d'agarose sont lavés deux fois pendant 30 min sur de la glace dans du TE/PMSF (10 mM Tris- HC1, pH8,0 ; 1 mM EDTA ; 100 μM PMSF). Puis, on lave les blocs d'agarose deux fois pendant 30 min sur de la glace dans du TE (10 mM Tris-HCL pH8,0 ; 1 mM EDTA). Chaque bloc d'agarose est coupé en huit fines tranches, et seize tranches sont mises ensemble dans un seul tube. Les blocs sont alors incubés dans une solution de pré-incubation (20 μM Spermidine, 5 mM DTT, tampon de restriction lx), pendant 40 min sur de la glace. On change le tampon de pré-incubation une fois et on l'incube de nouveau pendant 40 min.
Les digestions partielles sont effectuées dans un tampon de digestion (2 mM Spermidine, 0,5 mM DTT, 0,02 μg BSA, tampon de restriction 0,5x) pendant 30 min à 4°C, sans enzyme de restriction, puis on ajoute pendant 2 h à 37°C, 0,1 ; 0,25 ; 0,4 ou 0,5 d'unité d'EcoRI (life Tech) par tube. La digestion partielle est arrêtée par remplacement du tampon de digestion par 200 μl EDTA 0,5 M, les tubes étant placés sur de la glace. Les blocs d'agarose sont alors placés sur un gel d'agarose 1 % SeaKem GTG (FMC), 0,5x tampon TBE. On effectue alors le gel d'électrophorèse en champs puisé avec les conditions suivantes : temps de pulsation initial : 90 secondes, temps de pulsation final : 90 secondes,
6 V/cm, angle inclus : 120 °, durée de gel : 16 h, 12°C.
On coupe la région comprise entre 50 et 200 kb à partir du gel, et on la sépare en trois morceaux (50-100 kb, 100-150 kb, 150-200 kb). Le gel pour le clonage ne doit pas être teinté avec le bromure d'étidium.
Ces blocs d'agarose sont placés dans un nouveau gel d'agarose 1 % SeaKem GTG (FMC), 0,5x tampon TBE. On effectue un nouveau gel d'électrophorèse en champs puisé avec les conditions suivantes : temps initial de pulsation : 5 secondes, temps final de pulsation : 5 secondes,
4 V/cm, angle inclus : 120°, durée de gel : 15 h, 12°C. On découpe de nouveau, sans avoir teinté PADN avec du bromure d'étidium, la région comprise entre 50 et 200 kb et on la sépare en trois morceaux (50-100 kb, 100-150 kb, 150-200 kb).
Les morceaux d'agarose sont coupés en petits morceaux d'environ 100 mg chacun. L'agarose est incubé à 67°C pendant 10 min puis refroidi à 42°C, et on ajoute 1 μl de Beta-Agarase (FMC, lU/μl). On incube pendant 30 min à 42°C, et on effectue une dénaturation de la Beta-Agarase après digestion complète de l'agarose par incubation pendant 10 min à 67°C, puis incubation sur glace.
150 ng de vecteurs d'ADN digérés par EcoRI et des phophorylés (CEP, Roche) sont utilisés pour la construction de la banque de BAC. On utilise le vecteur pBelaBAC-Kan (Mozo et al., Mol. Gen. Genêt., 1998, 258, 562-70) pour la construction de ladite banque. On incube donc 150 ng dudit vecteur avec 150 ng d' inserts d'ADN, dans un tampon de ligation lx avec 5 unités de Ligase (USB 1 unité/μl) pendant 16 h à 12°C.
Le tampon de ligation est le tampon recommandé par le fabricant. La transformation est effectuée en ajoutant 5 μl de la réaction de ligation à 40 μl de cellules électrocompétentes DH10B, et l'électroporation est effectuée dans un électroporateur Life Tech avec les conditions suivantes :
- 330 μF, 4 kΩ, DC Volts :lowΩ, taux de charge : rapide, cuvette d'électroporation 1,5 mm. On ajoute 1 mm de milieu de culture SOC aux cellules et on incube pendant 45 min à 37°C. On étale ensuite les cellules sur une boîte contenant du LB agar et qui contient également 30 g/ml de kanamicine, 100 μg/ml d'IPTG et 40 μg/ml d'X- Gal. On sélectionne les cellules recombinantes qui sont blanches par rapport aux cellules bleues, et on caractérise la banque de BAC en effectuant des cartes de restriction de chacun des clones ainsi obtenus. Exemple 2 : Annotations et analyse de la séquence génomique de Listeria monocytogenes
La bio-informatique a un rôle clé dans les trois phases d'un projet génome : suivi des inserts réalisés par la méthode de séquençage aléatoire, phase de finition de la séquence génome, et annotations. Les Inventeurs ont développé un logiciel complet qui permet de répondre à ces trois exigences : GMP-Tool-box (GMPTB).
Suivi : Durant la procédure de séquençage aléatoire, GMPTB extrait des fichiers résultats (format Phrap) toutes les caractéristiques nécessaires à l'assemblage (nombre de contigs, nombre de séquences...) et les affiche dans un tableau. Ce tableau peut être utilisé pour créer des graphiques qui montrent la progression de cette méthode et qui permettent une identification rapide des problèmes d'assemblage. De façon importante, GMPTB permet la comparaison entre les résultats d'assemblage et crée une page HTML afin de décrire la relation entre les nouveaux et les anciens contigs (fusion, création...).
Phase de finition de la séquence : Différentes stratégies sont utilisées par GMPTB pour prédire les liaisons entre les contigs. GMPTB recherche en particulier tous les clones permettant les liaisons, sur la base de la localisation et de l'orientation des séquences terminales. Il peut aussi indiquer les misassemblages.
GMPTB peut aussi prédire les liaisons, sur la base de comparaisons de génomes, en recherchant les similarités entre les extrémités des contigs et d'autres séquences génomiques (haut niveau nucléotidique et des acides aminés).
Annotations : GMPTB permet de commencer l'annotation durant la phase terminale. De fait, GMPTB crée un fichier individuel de protéines (IPF), pour chaque phase ouverte de lecture (ORF) lors de l'assemblage. Ce sont des fichiers texte, dans un format spécifique qui contient trois catégories de champs :
- les champs minimums contiennent un numéro d'identification, un numéro de version, la localisation et les séquences. La séquence nucléotidique exportée correspond à la séquence de la phase ouverte de lecture avec 500 bases additionnelles avant le premier codon stop et 200 bases additionnelles après le second stop. le champ automatique contient des résultats ajoutés par différents programmes à l'IPF. Il concerne la séquence d'ADN (recherche de sites de liaisons de ribosomes, promoteurs ou terminateurs, capacité codante... ) et la séquence protéique prédite (homologie, domaine... ).
- le champ manuel contient des résultats et des commentaires ajoutés par les utilisateurs. Après un nouvel assemblage, GMPTB extrait toutes les ORF et crée de nouveaux IPF selon les séquences IPF dérivés des assemblages précédents. GMPTB reconnaît les IPF modifiés, qui sont les seuls utilisés pour une nouvelle analyse automatique après chaque assemblage. La spécificité de cette stratégie est que l'annotation des DPF est indépendante de l'étape de l'assemblage, à moins que sa séquence ne soit modifiée. Les IPF sont reliés à une banque de donnée génomique Sybases (du modèle SubtiList) et sont accessibles grâce à l'intermédiaire d'un serveur web. Ils peuvent être modifiés et annotés par différents Inventeurs durant la phase de projet de génome. Dépôt de matériel biologique
Les organismes suivants ont été déposés le 11 Avril 2000 à la Collection
Nationale de Cultures de Microorganismes (CNCM), 25 rue du Docteur Roux, 75724 Paris Cedex 15, France, selon les dispositions du Traité de Budapest :
Souche de Listeria monocytogenes EGD-e, Numéro d'ordre 1-2440 ;
- Banque BAC d'ADN de Listeria (145 clones), LM-baclib, Numéro d'ordre I-
2439. Ladite banque BAC (1-2439) a été réalisée dans la souche E.coli DH10B (Grant et al., PNAS, 87, 4645, 1990), construite après digestion partielle de l'ADN de Listeria monocytogenes par l'enzyme EcoRI dans le vecteur pBelaBAC-Kan (Mozo et al., Mol. Gen. Genêt., 1998, 258, 562-70).
TABLEAU 1
SeqlD Nom Localisationde la séquence Fonction nucléique de l'ORF sur la SEQ ID N° 1
SeqlD n° 2 LM-1000.1 From 589066 to 589362 Unknown, pepdidoglycan bound protein (LPXTG motif)
SeqlD n° 3 LM-1002.1 From 587012 to 589033 Unknown, similar to internalin protein
SeqlD n° 4 LM-1050.1 From 2907153 to 2909708 Unknown, LPXTG protein with LRR repeats
SeqlD n° 5 LM-1179.1 From 761552 to 763468 Unknown, putative peptidoglycan bound protein (LPXTG motif)
SeqlD n° 6 LM-118.2 From 2787416 to 2788363 Unknown, peptidoglycan anchored protein (LPXTG motif)
SeqlD n° 7 L -1235.1 From 875721 to 881855 Unknown, surface protein
(LPXTG motif)
SeqlD n° 8 LM-1248.1 From 865530 From 865530 unknown, surface protein
(LPXTG motif)
SeqlD n° 9 LM-1248.1 From 865530 From 865530 unknown, surface protein
(LPXTG motif)
SeqlD n° 10 LM-1305.1 From 919020 to 920408 Unknown, similar to wall associated protein precursor (LPXTG motif)
SeqlD n° 11 LM-1490.1 From 649864 to 651633 Unknown, similar to internalin proteins
SeqlD n° 12 LM-1514.1 From 664242 to 668990 Unknown, peptidoglycan bound protein (LPXTG motif) similar to adhesin
SeqlD n° 13 LM-1660.3 From 501312 to 501617 Unknown SeqlD n° 14 LM-1738.1 From 547520 to 549337 Unknown, similar to internalin proteins
SeqlD n° 15 L -1756.2 From 2679599 to 2681125 Unknown, surface protein
(GW repeat) similar to N- acetylmuramidase
SeqlD n° 16 LM-1778.1 From 1442368 to 1443687 Unknown, putative peptidoglycan bound protein (LPXTG motif)
SeqlD n° 17 LM-1972.3 From 1313654 to 1315435 Unknown, similar to internalin proteins SeqlD n° 18 LM-1974.3 From 1315767 to 1317563 Unknown, similar to internalin proteins
SeqlD n° 19 LM-2137.2 From 360936 to 366272 Unknown, similar to internalin proteins
SeqlD n° 20 LM-229.1 From 1170002 to 1171621 Unknown, putative interanlin, similar to InlA
SeqlD n° 21 LM-2323.1 From 344850 to 346049 Unknown, similar to surface proteins
SeqlD n° 22 LM-2435.1 From 159663 to 161378 unknwon, surface anchored protein (LPXTG motif)
SeqlD n° 23 LM-2438.1 From 157089 to 159470 unknwon, surface anchored protein (LPXTG motif)
SeqlD n° 24 LM-2503.1 From 2108500 to 2109603 Unknown, putative cell surface protein, similar to internalin proteins
SeqlD n° 25 L -2504.1 From 2106329 to 2108209 Unknown, putative peptidoglycan bound protein (LPXTG motif)
SeqlD n° 26 LM-3009.3 From 1149887 to 1152475 unknown, similar to fibrinogen-binding protein
(LPXTG motif)
SeqlD n° 27 LM-311.2 From 131419 to 133773 Unknwon, similar to 5 - nucleotidase (LPXTG motif)
SeqlD n° 28 LM-3144.1 From 351459 to 355505 Unknown, similar to cell surface proteins (LPXTG motif)
SeqlD n° 29 LM-3369.1 From 1869532 to 1872243 Unknown, putative peptidoglycan bound protein (LPXTG motif)
SeqlD n° 30 LM-3418.2 From 1717193 to 1722328 unknown, peptidoglycan linked protein (LPxTG)
SeqlD n° 31 LM-3477.1 From 828168 to 830108 Unknown, similar to internalin
SeqlD n° 32 LM-3609.1 From 1106041 to 1107759 unknown, similar to
AUTOLYSIN (EC 3.5.1.28)
(N-ACETYLMURAMOYL-
L-ALANINE AMIDASE)
SeqlD n° 33 LM-3691.2 From 2162323 to 2164011 Unknown, putative peptidoglycan bound protein (LPXTG motif)
SeqlD n° 34 LM-3700.2 From 2653211 to 2657803 Unknwon, peptidoglycan anchored protein (LPXTG motif)
SeqlD n° 35 LM-3752.3 From 357775 to 359676 Unknown, similar to internalin
SeqlD n° 36 L -757.1 From 2544267 to 2545433 Unknown, similar to internalin proteins SeqlD n° 37 LM-814.2 From 2264772 to 2268230 Unknown, putative peptidoglycan bound protein (LPXTG motif)
SeqlD n° 38 LM-816.2 From 2259753 to 2264591 Unknown, putative peptidoglycan bound protein (LPXTG motif)
SeqlD n° 39 LM-894.1 From 2469093 to 2471915 Unknown, similar to internalin proteins SeqlD n° 40 LM-966.1 From 173309 to 174556 Unknown, cell wall anchored protein SeqlD n° 41 LM-973.1 From 169510 to 172008 Unknwon, similar to internalin proteins SeqlD n° 42 L -133.1 From 1230773 to 1232308 Unknown, similar to cobyric acid synthase CbiP SeqlD n° 43 LM-134.1 From 1229967 to 1230773 Unknown, similar to cobalt transport ATP-binding protein CbiO
SeqlD n° 44 LM-135.1 From 1229277 to 1229954 Unknown, similar to cobalt transport protein Q SeqlD n° 45 LM-136.1 From 1228994 to 1229290 Unknown, similar to putative cobalt transport protein CbiN
SeqlD n° 46 LM-137.1 From 1228263 to 1228997 Unknown, similar to cobalamin biosynthesis protein M
SeqlD n° 47 LM-138.1 From 1227556 to 1228266 Unknown, similar to S- adenosyl-methionine: precorrin-2 methyltransferase
SeqlD n° 48 LM-139.1 From 1226778 to 1227563 unknown, similar to anaerobic Cobalt
Chelatase In Cobalamin
Biosynthesis
SeqlD n° 49 LM-141.1 From 1225300 to 1226781 Unknown, similar uroporphyrinogen-lll methyltransferase/uroporph yrinogen-lll synthase
SeqlD n° 50 LM-142.1 From 1224548 to 1225300 Unknown, similar to cobalamin biosynthesis J protein CbiJ
SeqlD n° 51 LM-143.1 From 1223826 to 1224551 Unknown, similar to precorrin methylase
SeqlD n° 52 LM-144.1 From 1222798 to 1223829 Unknown, similar to cobalamin biosynthesis protein G CbiG
SeqlD n° 53 LM-145.1 From 1222062 to 1222811 Unknown, similar to precorrin-3 methylase
SeqlD n° 54 LM-146.1 From 1221487 to 1222056 Unknown, similar to precorrin decarbocylase
SeqlD n° 55 LM-147.1 From 1220901 to 1221497 Unknown, similar to precorrin methylase SeqlD n° 56 L -149.1 From 1219783 to 1220904 Unknown, similar to cobalamin biosynthesis protein CbiD
SeqlD n° 57 LM-150.1 From 1219135 to 1219767 Unknown, similar to precorrin isomerase SeqlD n° 58 LM-151.1 From 1218175 to 1219122 Unknown, similar to cobalamine synthesis protein CbiB
SeqlD n° 59 LM-152.1 From 1216963 to 1217322 FALSE ORF SeqlD n° 60 LM-181.1 From 1196097 to 1197182 unknown, similar to
Salmonella typhimurium
CobD protein and to histidinol-phosphate aminotransferase
SeqlD n° 61 LM-207.1 From 1180152 to 1181036 Regulatory protein similar to Salmonella typhimurium
PocR protein
SeqlD n° 62 LM-209.1 From 1179168 to 1179743 unknown, similar to alpha- ribazole-5 -phosphatase
SeqlD n° 63 LM-210.1 From 1178421 to 1179167 unknown, highiy similar to cobalamin (5 - phosphatase) synthetase
SeqlD n° 64 LM-212.1 From 1177862 to 1178419 unknown, similar to bifunctional cobalamin biosynthesis protein CopB,
(cobinamide kinase; cobinamide phosphatase guanylyltransferase)
SeqlD n° 65 LM-1.3 From 2710869 to 2712755 Unknown, similar to NADH dehydrogenase
SeqlD n° 66 LM-10.1 From 2718312 to 2719055 Unknown
SeqlD n° 67 LM-100.1 From 2775644 to 2776486 Unknown, similar to aldo/keto reductase
SeqlD n° 68 L -1003.1 From 586329 to 586994 Unknown
SeqlD n° 69 LM-1004.1 From 585015 to 585962 Unknown, similar to B. subtilis DeoR transcriptional regulator
SeqlD n° 70 LM-1005.1 From 583507 to 584757 Unknown, similar to putative NAD(P)- dependent oxidoreductase
SeqlD n° 71 LM-1007. From 583072 to 583452 Unknown SeqlD n° 72 LM-1008.1 From 582526 to 583047 Unknown, similar to PTS
System, glucitol/sorbitol- specific enzyme II Cil component
SeqlD n° 73 LM-1009.1 From 581519 to 582505 Unknown, similar to PTS
System, glucitol/sorbitol- specific enzyme IIBC component
SeqlD n° 74 LM-101.1 From 2776532 to 2776777 unknown, similar to B. subtilis YaaL protein SeqlD n° 75 LM-1010.1 From 581150 to581500 Unknown, similar to PTS system, glucitol/sorbitol- specific enzyme IIA component
SeqlD n° 76 LM-1011.1 From 580119 to 581039 Unknown, similar to ABC transporter (binding protein)
SeqlD n° 77 LM-1012.1 From 578886 to 580079 Unknown, similar to penicillin-binding protein
SeqlD n° 78 L -1013.1 From 577632 to 578648 Unknown, similar to tagatose-1 ,6-diphosphate aldolase
SeqlD n° 79 LM-1015.1 From 576403 to 577584 Unknown, similar to N-acyl-
L-amino acid amidohydrolase
SeqlD n° 80 LM-1017.1 From 575166 to 576437 Unknown, similar to N- carbamyi-L-amino acid amidohydrolase
SeqlD n° 81 LM-1018.1 From 573740 to 575062 Unknown, similar to 6- phospho-beta-glucosidase
SeqlD n° 82 LM-102.1 From 2776792 to 2777388 unknown, highiy similar to recombination protein recR
SeqlD n° 83 LM-1020.1 From 572600 to 573568 Unknown, similar to transcription regulator (Lacl family)
SeqlD n° 84 LM-1022.1 From 571204 to 572559 Unknown, similar to unknown proteins
SeqlD n° 85 L -1023.1 From 570916 to 571185 Unknown, similar to unknown proteins
SeqlD n° 86 LM-1024.1 From 570008 to 570838 Unknown
SeqlD n° 87 LM-1025.1 From 569094 to 569948 Unknown
SeqlD n° 88 LM-1029.1 From 567030 to 569081 Unknown
SeqlD n° 89 LM-103.1 From 2777497 to 2777814 Unknown, highiy similar to
B. subtilis YaaK protein
SeqlD n° 90 LM-1031.1 From 565764 to 567014 Unknown, conserved hypothetical protein similar to putative glucosaminyltransferase
SeqlD n° 91 LM-1032.1 From 564257 to 565753 Unknown, hypothetical secreted protein SeqlD n° 92 LM-1033.1 From 562789 to 564264 Unknown, transmembrane protein SeqlD n° 93 LM-1036.1 From 561819 to 562559 Unknown, similar to transcription regulator
(TipA from Streptomyces coelicolor)
SeqlD n° 94 LM-1037.1 From 560443 to 561774 Unknown SeqlD n° 95 LM-1040.2 From 2915744 to 2916097 Unknown SeqlD n° 96 LM-1041.2 From 2915219 to 2915641 Unknown similar to transcriptional regulator
(MarR family) SeqlD n° 97 LM-1043.1 From 2914057 to 2915205 Unknown, similar to efflux proteins
SeqlD n° 98 LM-1045.1 From 2912595 to 2913686 unknown, highiy similar to phosphoserine aminotransferase
SeqlD n° 99 LM-1047.1 From 2911415 to 2912602 unknown, similar to D-3- phosphoglycerate dehydrogenase
SeqlD n° 100 LM-1048.1 From 2910085 to 2911326 unknown, similar to an hypothetical protein from
Thermotoga maritima
SeqlD n° 101 LM-1049.1 From 2909753 to 2910076 unknown SeqlD n° 102 LM-1052.1 From 2904598 to 2906940 Unknown, amino-terminal domain similar to transcription regulators
SeqlD n° 103 LM-1054.1 From 2903200 to 2904402 unknown, similar to carboxypeptidase SeqlD n° 104 LM-1055.1 From 2901800 to 2903200 Unknown, similar to transmembrane efflux protein
SeqlD n° 105 LM-1056.1 From 2900594 to 2901784 unknown, similar to peptidases
SeqlD n° 106 LM-1057.1 From 2899313 to 2900506 unknown, similar to transport protein
SeqlD n° 107 LM-1058.1 From 2898535 to 2899266 Unknown, similar to reductases
SeqlD n° 108 LM-1059.1 From 2897847 to 2898374 Unknown, similar to transcriptional regulator
SeqlD n° 109 LM-1060.1 From 2897116 to 2897823 Unknown SeqlD n° 110 LM-1061.1 From 2896241 to 2897059 unknown, similar to D- alanyl-D-alanine carboxypeptidase
SeqlD n° 111 LM-1064.1 From 2894510 to 2895883 Unknown, similar to
GTPase
SeqlD n° 112 LM-1066.1 From 2892596 to 2894485 unknown, highiy similar to
GidA protein
SeqlD n° 113 LM-1067.2 From 2892057 to 2892437 Unknown, hypothetical secreted protein
SeqlD n° 114 LM-1069.3 From 434945 to 435817 Unknown
SeqlD n° 115 LM-107.1 From 2777844 to 2779583 unknown, highiy similar to
DNA polymérase III
(gamma and tau subunits)
SeqlD n° 116 LM-1070.1 From 433153 to 434745 Unknown, similar to phosphoenolpyruvate synthase (N-terminal part)
SeqlD n° 117 LM-1074.1 From 429630 to 432095 internalin SeqlD n° 118 LM-1075.1 From 428981 to 429403 Unknown SeqlD n° 119 LM-1076.1 From 428576 to 428968 Unknown SeqlD n° 120 LM-1077.1 From 428127 to 428507 Unknown, similar to B. subtilis YyaH protein
SeqlD n° 121 LM-1079.1 From 427104 to 428111 Unknown, similar to phosphate transport protein SeqlD n° 122 LM-108.1 From 2779910 to 2780374 Unknown SeqlD n° 123 LM-1080.1 From 426597 to 426995 Unknown SeqlD n° 124 LM-1081.1 From 426190 to 426606 Unknown SeqlD n° 125 LM- 1082.1 From 424130 to 426064 Unknown, similar to transcriptional antiterminator (BglG family)
SeqlD n° 126 LM-1083.1 From 421469 to 424096 Unknown, highiy similar to
E. col YbgG protein, a putative sugar hydrolase
SeqlD n° 127 LM-1084.1 From 420335 to 421447 Unknown, similar to fructose-specific phosphotransferase enzyme IIC
SeqlD n° 128 LM-1085.1 From 419999 to 420331 Unknown, similar to fructose-specific phosphotransferase enzyme IIB
SeqlD n° 129 LM-1088.1 From 419544 to 420002 Unknown, similar to phosphotransferase
System enzyme HA
SeqlD n° 130 LM-1089.1 From 418891 to 419373 Unknown, similar to unknown proteins SeqlD n° 131 LM-109.1 From 2780933 to 2782297 Unknown, similar to PTS
System, cellobiose-specific enzyme IIC
SeqlD n° 132 LM-1091.1 From 417963 to 418763 Unknown, similar to 1- pyrroline-5-carboxylate reductase (ProC)
SeqlD n° 133 LM-1092.1 From 417535 to 417960 Unknown, weakiy similar to blasticidin S- acetyltransferase
SeqlD n° 134 LM-1093.1 From 416796 to 417479 Unknown, similar to L. monocytogenes extracellular P60 protein
SeqlD n° 135 LM-1094.1 From 416203 to 416652 Unknown SeqlD n° 136 LM-1097.1 From 415224 to 416168 Unknown, highiy similar to
B. subtilis YqfA protein
SeqlD n° 137 LM-1098.1 From 414928 to 415227 Unknown SeqlD n° 138 LM-1099.1 From 414095 to 414760 Unknown, similar to uracil-
DNA glycosylase
SeqlD n° 139 LM-11.1 From 2719056 to 2719775 Unknown SeqlD n° 140 LM-1100.1 From 412849 to 413964 Unknown, low température requirement protein A
SeqlD n° 141 LM-1101.1 From 412457 to 412825 Unknown SeqlD n° 142 LM-1102.1 From 411992 to 412363 Unknown, similar to B. subtilis YhdG protein
SeqlD n° 143 LM-1105.1 From 409944 to 411860 Unknown, similar to B. subtilis lolD protein, to acetolactate synthase
SeqlD n° 144 LM-1107.1 From 408954 to 409931 Unknown, similar to B. subtilis lolC protein and to fructokinase Seq I D n ° 145 LM- 1108.1 From 408116 to 408937 Unknown, similar to B. subtilis lolB protein SeqlD n° 146 LM-1110.2 From 406637 to 408103 Unknown, highiy similar to
B. subtilis methylmalonate- semialdehyde dehydrogenase lolA
SeqlD n° 147 LM-1112.2 From 405680 to 406441 Unknown, similar to B. subtilis transcription repressor of myo-inositol catabolism operon loIR
SeqlD n° 148 LM-1114.1 From 405221 to 405607 Unknown SeqlD n° 149 LM-1115.3 From 404365 to 404994 Unknown SeqlD n° 150 LM-1116.4 From 807976 to 810792 Unknown, similar to transcriptional regulator
(NifA/NtrC family)
SeqlD n° 151 LM-1117.1 From 807255 to 807689 Unknown, similar to mannose-specific phosphotransferase system (PTS) component
IIA
SeqlD n° 152 LM-1118.1 From 806770 to 807255 Unknown, similar to mannose-specific phosphotransferase system (PTS) component
IIB
SeqlD n° 153 LM-112.1 From 2783466 to 2784107 Unknown SeqlD n° 154 LM-1121.1 From 805784 to 806596 Unknown, similar to mannose-specific phosphotransferase system (PTS) component
IIC
SeqlD n° 155 LM-1122.1 From 804887 to 805765 Unknown, similar to mannose-specific phosphotransferase system (PTS) component
IID
SeqlD n° 156 LM-1123.1 From 804382 to 804729 Unknown
SeqlD n° 157 LM-1125.1 From 803745 to 804212 Unknown
SeqlD n° 158 LM-1126.1 From 803201 to 803548 Unknown
SeqlD n° 159 LM-1127.1 From 802356 to 802739 Unknown
SeqlD n° 160 LM-1128.1 From 801336 to 802202 Unknown, similar to transcription regulator
(repressor)
SeqlD n° 161 LM-1129.1 From 800965 to 801297 Unknown SeqlD n° 162 LM-113.1 From 2784354 to 2784674 unknown, similar to hypothetical proteins
SeqlD n° 163 LM-1130.1 From 799996 to 800925 Unknown, conserved hypothetical protein
SeqlD n° 164 LM-1131.1 From 798828 to 799817 Unknown, similar to alcohol dehydrogenase
SeqlD n° 165 LM-1132.1 From 798141 to 798788 Unknown, similar to transcription regulator
SeqlD n° 166 LM-1133.1 From 797353 to 798105 Unknown SeqlD n° 167 LM-1134.1 From 796075 to 797193 Unknown, similar to transcriptional regulator
(Lacl family)
SeqlD n° 168 LM-1135.1 From 795034 to 796062 Unknown, similar to alpha-
1 ,6-mannanase SeqlD n° 169 LM-1136.1 From 793777 to 795030 Unknown, similar to sugar
ABC transporter, periplasmic sugar-binding protein
SeqlD n° 170 LM-1137.1 From 792879 to 793754 Unknown, similar to ABC transporter, permease protein SeqlD n° 171 LM-1138.1 From 791977 to 792867 Unknown, similar to putative sugar ABC transporter, permease protein
SeqlD n° 172 LM-1139.1 From 790662 to 791960 Unknown SeqlD n° 173 LM-114.1 From 2784805 to 2786319 Unknown, highiy similar to gluconate kinase
SeqlD n° 174 LM-1140.1 From 789520 to 790509 Unknown, similar to lipoate-protein ligase
SeqlD n° 175 LM-1141.1 From 788642 to 789514 Unknown, similar to unknown proteins
SeqlD n° 176 LM-1142.1 From 787320 to 788576 Unknown, similar to
ATP/GTP-binding protein
SeqlD n° 177 LM-1143.1 From 786372 to 786992 Unknown, similar to unknown proteins
SeqlD n° 178 LM-1144.1 From 785754 to 786362 Unknown SeqlD n° 179 LM-1146.1 From 784788 to 785747 Unknown SeqlD n° 180 LM-1147.1 From 783901 to 784788 Unknown SeqlD n° 181 LM-1149.1 From 782794 to 783774 Unknown, similar to hypothetical proteins
SeqlD n° 182 LM-1151.1 From 781896 to 782801 Unknown; Similar to ABC transporter (ATP-binding protein)
SeqlD n° 183 LM-1152.1 From 781103 to 781924 unknown, similar to unknown proteins SeqlD n° 184 LM-1153.2 From 780377 to 780988 Unknown, weakiy similar to a bile acid 7-alpha dehydratase
SeqlD n° 185 LM-1154.1 From 779616 to 780296 unknown, similar to transcription regulator
Crp/Fnr family
SeqlD n° 186 LM-1155.1 From 778654 to 779490 Unknown, weakiy similar to a putative haloacetate dehalogenase
SeqlD n° 187 LM-1156.1 From 778262 to 778558 Unknown SeqlD n° 188 LM-1157.1 From 777692 to 778207 Unknown SeqlD n° 189 LM-1158.1 From 777209 to 777496 Unknown SeqlD n° 190 LM-1159.1 From 776761 to 777102 unknown SeqlD n° 191 LM-1160.1 From 776398 to 776697 Unknown, hypothetical SeqlD n° 192 LM-1161.1 From 775744 to 776247 Unknown SeqlD n° 193 LM-1164.1 From 773715 to 775715 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 194 LM-1165.1 From 772996 to 773700 Unknown SeqlD n° 195 LM-1166.1 From 772310 to 772999 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 196 LM-1167.1 From 771949 to 772326 Unknown, similar to transcriptional regulator
(GntR family)
SeqlD n° 197 LM-1168.1 From 771026 to 771685 Unknown, similar to putative transcription regulator
SeqlD n° 198 LM-1169.1 From 769639 to 771012 Unknown, similar to 6- phospho-beta-glucosidase
SeqlD n° 199 LM-117.1 From 2786435 to 2787373 Unknown, secreted protein with 1 GW repeat
SeqlD n° 200 LM-1171.1 From 767766 to 769619 Unknown, similar to phosphotransferase system (PTS) beta- glucoside-specific enzyme
IIABC component
SeqlD n° 201 LM-1172.1 From 766810 to 767742 Unknown SeqlD n° 202 LM-1173.1 From 766351 to 766797 Unknown, similar to ribose
5-phosphate isomerase
SeqlD n° 203 LM-1174.1 From 765683 to 766354 Unknown, similar to
Ribulose-5-Phosphate 3-
Epimerase
SeqlD n° 204 LM-1175.1 From 764465 to 765469 Unknown, similar to transcriptional regulator
(Lacl family)
SeqlD n° 205 LM-1176.1 From 763798 to 764307 Unknown, similar to transcription regulator
SeqlD n° 206 LM-1181.1 From 760455 to 760859 Unknown SeqlD n° 207 LM-1182.2 From 759972 to 760310 Unknown SeqlD n° 208 LM-1183.2 From 759479 to 759958 Unknown
SeqlD n° 209 LM-1184.3 From 2916291 to 2916890 Unknown, similar to yeast protein Frm2p involved in fatty acid signaling
SeqlD n° 210 LM-1186.1 From 2916946 to 2917266 Unknown, similar to thioredoxin
SeqlD n° 211 LM-1189.1 From 2917389 to 2918051 Unknown, similar to phosphoglucomutase
SeqlD n° 212 LM-119.2 From 1239842 to 1240201 unknown SeqlD n° 213 LM-1190.1 From 2918048 to 2919169 unknown; similar to unknown proteins
SeqlD n° 214 LM-1195.1 From 2919166 to 2921343 Unknown, similar to a maltose phosphorylase
SeqlD n° 215 LM-1196.1 From 2921340 to 2922371 Unknown, similar to oxidoreductases
SeqlD n° 216 LM-1198.1 From 2922429 to 2923223 Unknown, highiy similar to an E. coli protein SeqlD n° 217 LM-1199.1 From 2923236 to 2924288 Unknown, similar to alcohol dehydrogenase SeqlD n° 218 LM-1201.1 From 2924307 to 2925158 Unknown, similar to sugar
ABC transporter permease protein
SeqlD n° 219 LM-1202.1 From 2925145 to 2926026 Unknown, similar to sugar
ABC transporter permease protein
SeqlD n° 220 LM-1203.1 From 2926101 to 2927393 Unknown, similar to sugar binding protein (ABC transporter)
SeqlD n° 221 LM-1205.1 From 2927390 to 2928631 Unknown, similar to
Sucrose phosphorylase
SeqlD n° 222 LM-1206.1 From 2928668 to 2929090 unknown, weakiy similar to sucrose phosphorylase
SeqlD n° 223 LM-1208.1 From 2929315 to 2930340 Unknown, similar to transcriptional regulator
SeqlD n° 224 LM-1209.1 From 2930479 to 2932473 unknown SeqlD n° 225 LM-121.1 From 1239039 to 1239797 unknown, similar to rRNA methylase
SeqlD n° 226 LM-1210.1 From 2932609 to 2933148 Unknown, similar to unknown proteins
SeqlD n° 227 LM-1212.1 From 2933303 to 2934715 unknown, similar to transmembrane efflux proteins
SeqlD n° 228 LM-1213.1 From 2934756 to 2935070 Unknown, highiy similar to
B. subtilis YulD protein SeqlD n° 229 LM-1214.1 From 2935083 to 2935904 unknown, highiy similar to rhamnulose-1-phosphate aldolase
SeqlD n° 230 LM-1216.1 From 2935917 to 2937179 unknown, highiy similar to
L-rhamnose isomerase SeqlD n° 231 LM-1218.1 From 2937192 to 2938643 unknown; similar to rhamnulokinase SeqlD n° 232 LM-122.1 From 1237934 to 1239013 unknown, similar to endo-
1,4-beta-glucanase and to aminopeptidase
SeqlD n° 233 LM-1220.1 From 2938662 to 2939930 Unknown, similar to sugar transport proteins
SeqlD n° 234 LM-1221.1 From 2940053 to 2941033 Unknown, similar to AraC- type regulatory protein
SeqlD n° 235 LM-1222.1 From 2941083 to 2941544 unknown SeqlD n° 236 LM-1223.2 From 2941599 to 2942219 unknown, highiy similar to
B. subtilis Jag protein
SeqlD n° 237 LM-1224.3 From 2942216 to 2943079 Unknown, highiy similar to
B. subtilis SpolllJ protein
SeqlD n° 238 LM-1225.2 From 884170 to 885270 Unknown, similar to excinuclease ABC, chain C
(UvrC)
SeqlD n° 239 LM-1228.1 From 882962 to 884065 Unknown, similar to B. subtilis YxjH and YxjG proteins SeqlD n° 240 LM-123.1 From 1236836 to 1237822 unknown, similar to N- acetylmuramoyl-L-alanine amidase (autolysin)
SeqlD n° 241 LM-1230.1 From 882328 to 882705 unknown, conserved hypothetical protein
SeqlD n° 242 LM-1231.1 From 881992 to 882249 unknown, similar to B. subtilis protein YsdA
SeqlD n° 243 LM-1239.1 From 872616 to 875258 unknown, similar to cation
(clacium) transporting
ATPase
SeqlD n° 244 LM-1241.1 From 871940 to 872410 Unknown SeqlD n° 245 LM-1242.1 From 870587 to 871807 unknown, similar to
Tetracycline résistance protein
SeqlD n° 246 LM-1243.1 From 869095 to 870480 unknown, highiy similar to hexose phosphate transport protein
SeqlD n° 247 LM-1245.1 From 867163 to 868800 Unknown, ABC transporter
(ATP binding protein)
SeqlD n° 248 LM-1246.1 From 866577 to 866990 unknown, similar to B. subtilis YrkR protein
SeqlD n° 249 LM-1249.1 From 864791 to 865537 unknown
SeqlD n° 250 LM-125.1 From 1235670 to 1236539 unknown, similar to N- acetylmuramoyl-L-alanine amidase (autolysin)
SeqlD n° 251 LM-1250.1 From 863798 to 864688 Unknown; similar to transcriptional regulator
SeqlD n° 252 LM-1251.1 From 863322 to 863606 unknown, similar to transposase
SeqlD n° 253 LM-1254.1 From 861731 to 862642 unknwon
SeqlD n° 254 LM-1255.1 From 859656 to 861617 unknown, highiy similar to fructose-1,6- bisphosphatase
SeqlD n° 255 LM-1257.1 From 855759 to 859406 unknown, highiy similar to pyruvate-flavodoxin oxidoreductase
SeqlD n° 256 LM-1258.1 From 854745 to 855581 unknown, similar to transposases
SeqlD n° 257 LM-1259.1 From 854443 to 854748 unknown, similar to transposases
SeqlD n° 258 LM-1261.1 From 852704 to 854338 Unknown, similar to transport protein
SeqlD n° 259 LM-1262.1 From 851225 to 852505 unknown, similar to 3- hydroxy-3-methylglutaryl- coenzyme a reductase
SeqlD n° 260 LM-1263.1 From 850216 to 851184 unknown
SeqlD n° 261 LM-1265.1 From 849314 to 850138 unknown, similar to oxydoreductases
SeqlD n° 262 LM-1266.1 From 848804 to 849193 unknown, similar to transcriptional regulators
SeqlD n° 263 LM-1267.1 From 848126 to 848788 unknown SeqlD n° 264 LM-1268.1 From 847552 to 848109 unknown, some similarity to acatyltransferases
Seq D n° 265 LM-1269.1 From 846594 to 847442 unknown Seq D n° 266 LM-1271.2 From 843949 to 846579 unknown, similar to cation transporting ATPase
Seq D n° 267 LM-1273.1 From 939106 to 939819 Unknown, similar to transcription regulator
(GntR family)
Seq D n° 268 LM-1275.2 From 937739 to 939025 unknown, similar to PTS system, cellobiose-specific
IIC component
SeqlD n° 269 LM-1277.2 From 937202 to 937594 Unknown
SeqlD n° 270 LM-1278.1 From 936136 to 936603 unknown, similar to B. subtilis YdcK protein
SeqlD n° 271 LM-128.1 From 1233904 to 1234434 unknown, similar to unknown proteins
SeqlD n° 272 LM-1283.1 From 933959 to 936136 unknown, conserved hypothetical protein
SeqlD n° 273 LM-1285.1 From 932257 to 933882 unknown, similar to transport proteins
SeqlD n° 274 LM-1286.1 From 931451 to 932050 Indirect négative régulation of sigma B dépendant gène expression (serine phosphatase)
SeqlD n° 275 LM-1287.1 From 930671 to 931450 RNA polymérase sigma-37 factor (sigma-B)
SeqlD n° 276 LM-1288.1 From 930220 to 930693 sigma-B activity négative regulator RsbW
SeqlD n° 277 LM-1289.1 From 929892 to 930236 anti-anti-sigma factor
(antagonist of RsbW)
SeqlD n° 278 LM-129.1 From 1233366 to 1233815 unknown, similar to unknown proteins
SeqlD n° 279 LM-1290.1 From 928738 to 929742 unknown, highiy similar to serine phosphatase RsbU
SeqlD n° 280 LM-1291.1 From 928311 to 928721 unknown, highiy similar to positive régulation of sigma-B activity
Seq D n° 281 LM-1293.1 From 927952 to 928308 unknown, highiy similar to négative régulation of sigma-B activity
Seq D n° 282 LM-1294.1 From 927110 to 927946 unknown, highiy similar to positive regulator of sigma-
B activity
Seq D n° 283 LM-1295.1 From 926511 to 926858 Unknown, similar to B. subtilis YdcE protein Seq D n° 284 LM-1296.1 From 926229 to 926507 Unknown, similar to B. subtilis YdcD protein Seq D n° 285 LM-1297.1 From 924917 to 926023 Unknown, similar to alanine racemase Seq D n° 286 LM-1298.1 From 924542 to 924898 Unknown, similar to holo- acyl-carrier protein synthase SeqlD n° 287 LM-13.1 From 2719735 to 2720484 Unknown, similar to creatinine amidohydrolase SeqlD n° 288 LM-130.1 From 1232884 to 1233351 unknown, similar to unknown proteins SeqlD n° 289 LM-1300.1 From 923161 to 924540 unknown, similar to protoporphyrinogen IX and coproporphyrinogen III oxidase (HemY)
SeqlD n° 290 LM-1302.1 From 921537 to 923012 Unknown, similar to B. subtilis YbtB protein
SeqlD n° 291 LM-1303.1 From 921062 to 921544 unknown, similar to B. subtilis YdbS protein
SeqlD n° 292 LM-1304.1 From 920452 to 920928 unknown SeqlD n° 293 LM-1306.1 From 918135 to 918902 unknown SeqlD n° 294 LM-1307.1 From 917164 to 918117 unknown, similar to oxidoreductases
SeqlD n° 295 LM-1309.1 From 916436 to 917164 unknown, similar to B. subtilis NagB protein
(glucosamine-6-phosphate isomerase)
SeqlD n° 296 LM-131.1 From 1232272 to 1232838 unknown, similar to unknown protein
SeqlD n° 297 LM-1312.1 From 915090 to 916415 unknown, similar to PTS system, Lichenan-specific enzyme IIC component
SeqlD n° 298 LM-1313.1 From 914735 to 915070 unknown, similar to PTS system, beta-glucoside enzyme IIB component
SeqlD n° 299 LM-1314.1 From 914405 to 914734 Unknown, similar to PTS system enzyme IIA component
SeqlD n° 300 LM-1316.1 From 912372 to 914390 Unknown; Similar to transcriptional regulator
(antiterminator)
SeqlD n° 301 LM-1317.1 From 910998 to 912164 Unknown; similar to antibiotic résistance protein
SeqlD n° 302 LM-1318.1 From 910517 to 910870 Unknown, similar to B. subtilis YtcD protein
SeqlD n° 303 LM-1319.1 From 910173 to 910484 unknown SeqlD n° 304 LM-1320.1 From 909159 to 910157 unknown SeqlD n° 305 LM-1321.1 From 908499 to 909056 unknown SeqlD n° 306 LM-1322.1 From 907979 to 908467 unknown SeqlD n° 307 LM-1325.1 From 905962 to 907524 Unknown, similar to ATP- dependent RNA helicase
SeqlD n° 308 LM-1326.1 From 903837 to 905510 Unknown, similar to phosphomannomutase
SeqlD n° 309 LM-1327.1 From 902773 to 903840 unknown SeqlD n° 310 LM-1328.1 From 901837 to 902751 unknown SeqlD n° 311 LM-1329.1 From 900303 to 901835 unknown, similar to oligo-
1,6-glucosidase SeqlD n° 312 LM-1331.1 From 899454 to 900287 unknown, similar to sugar ABC transporter, permease protein -
SeqlD n° 313 LM-1332.1 From 898589 to 899467 unknown, similar to sugar
ABC transporter, permease protein
SeqlD n° 314 LM-1333.3 From 897273 to 898592 Unknown, similar to putative sugar ABC transporter, periplasmic sugar-binding protein,
SeqlD n° 315 LM-1335.3 From 1104048 to 1104851 unknown, highiy similar to teichoic acid translocation permease protein TagG
SeqlD n° 316 LM-1337.2 From 1102858 to 1103757 unknown, similar to métal binding protein
SeqlD n° 317 LM-1338.1 From 1099266 to 1102706 unknown, highiy similar to pyruvate carboxylase
SeqlD n° 318 LM-1339.1 From 1097786 to 1098988 unknown, similar to cell- division protein RodA and
FtsW
SeqlD n° 319 LM-1341.1 From 1097322 to 1097603 unknown, similar to B. subtilis YlaN protein
SeqlD n° 320 LM-1342.1 From 1096874 to 1097116 unknown, similar to B. subtilis Ylal protein
SeqlD n° 321 LM-1343.2 From 1095975 to 1096835 unknown
SeqlD n° 322 LM-1349.1 From 1093965 to 1095803 unknown, similar to GTP- binding ptotein TypA/BipA
(tyrosine phosphorylated protein A) from E. coli and
B. subtilis (YlaG)
SeqlD n° 323 LM-1351.1 From 1093000 to 1093773 unknown, similar to extragenic suppressor protein SuhB and to myo- inositol-1 (or 4)- monophosphatase
SeqlD n° 324 LM-1352.1 From 1092255 to 1092869 unknown, similar to B. subtilis YktB protein
SeqlD n° 325 LM-1353.1 From 1091110 to 1092054 unknown, similar to membrane and transport proteins
SeqlD n° 326 LM-1354.1 From 1090375 to 1091043 unknown, similar to ABC transporter (permease)
SeqlD n° 327 LM-1357.1 From 1088941 to 1090362 unknown, conserved hypothetical protein
SeqlD n° 328 LM-1358.1 From 1087409 to 1088854 unknown, similar to sensor protein histidine kinases (2 components regulatory
Systems)
SeqlD n° 329 LM-1359.1 From 1086772 to 1087434 unknown, similar to transcription response regulator
SeqlD n° 330 LM-1360.1 From 1086100 to 1086630 unknown SeqlD n° 331 LM-1361.1 From 1085806 to 1086078 unknown, similar to B. subtilis YktA protein
SeqlD n° 332 LM-1363.1 From 1084853 to 1085806 unknown, similar to L- lactate dehydrogenase
SeqlD n° 333 LM-1364.1 From 1084413 to 1084724 unknown SeqlD n° 334 LM-1367.1 From 1082822 to 1084225 unknown, highiy similar to dihydrolipoamide dehydrogenase, E3 subunit of pyruvate dehydrogenase complex
SeqlD n° 335 LM-1368.1 From 1081183 to 1082817 unknown, highiy similar to pyruvate dehydrogenase
(dihydrolipoamide acetyltransferase E2 subunit)
SeqlD n° 336 LM-1369.1 From 1080095 to 1081072 unknown, highiy similar to pyruvate dehydrogenase
(E1 beta subunit) SeqlD n° 337 LM-1370.1 From 1078977 to 1080092 unknown, highiy similar to pyruvate dehydrogenase
(E1 alpha subunit) SeqlD n° 338 LM-1371.1 From 1077592 to 1078143 unknown, similar to formylmethionine deformylase and to B. subtilis YkrB protein
SeqlD n° 339 LM-1372.1 From 1076989 to 1077543 unknown, similar to B. subtilis YdfE protein SeqlD n° 340 LM-1373.1 From 1075860 to 1076858 Unknown, similar to molybdopterin biosynthesis protein MoeB
SeqlD n° 341 LM-1374.1 From 1075360 to 1075848 unknown, similar to molybdenum cofactor biosynthesis protein B
SeqlD n° 342 LM-1376.1 From 1074324 to 1075325 unknown, similar to molybdenum cofactor biosynthesis protein A
SeqlD n° 343 LM-1377.1 From 1073813 to 1074295 unknown, similar to molybdenum cofactor biosynthesis protein C
SeqlD nά 344 LM-1379.1 From 1073552 to 1073800 unknown, similar to molybdopterin converting factor (subunit 1).
SeqlD n° 345 LM-1380.1 From 1073146 to 1073568 Unknown, similar to molybdopterin converting factor, subunit 2
SeqlD n° 346 LM-1381.1 From 1072664 to 1073149 unknown, similar to molybdopterin-guanine dinucleotide biosynthesis
MobB
SeqlD n° 347 LM-1383.1 From 1071462 to 1072685 unknown, similar to molybdopterin biosynthesis protein moeA SeqlD n° 348 LM-1385.1 From 1070597 to 1071367 unknown, similar to molybdate binding protein
SeqlD n° 349 LM-1386.1 From 1069821 to 1070492 Unknown, similar to molybdenum transport protein
SeqlD n° 350 LM-1387.1 From 1069156 to 1069818 unknown, similar to ABC transporter
SeqlD n° 351 LM-1388.1 From 1068594 to 1069175 unknown, weakiy similar to molybdopterin-guanine dinucleotide biosynthesis protein A
SeqlD n° 352 LM-1389.1 From 1067723 to 1068526 unknown, highiy similar to
B. subtilis YoaT protein
SeqlD n° 353 LM-1390.1 From 1066397 to 1067662 unknown
SeqlD n° 354 LM-1391.1 From 1064521 to 1066377 unknown, similar to phosphotransferase system (PTS) beta- glucoside-specific enzyme
IIABC
SeqlD n° 355 LM-1392.1 From 1063061 to 1064524 unknown, similar to glycerol kinase
SeqlD n° 356 LM-1394.1 From 1062108 to 1063064 unknown, similar to transketolase
SeqlD n° 357 LM-1396.1 From 1061291 to 1062115 unknown, similar to transketolase
SeqlD n° 358 LM-1398.3 From 1059872 to 1061275 unknown, similar to hypothetical proteins
SeqlD n° 359 LM-1401.1 From 235524 to 237020 lysyl-tRNA synthetase SeqlD n° 360 LM-1402.1 From 234414 to 235409 Unknwon, conserved hypothetical protein
SeqlD n° 361 LM-1404.1 From 233858 to 234337 unknown, similar to 7,8- dihydro-6- hydroxymethylpterin pyrophosphokinase
SeqlD n° 362 LM- 1405.1 From 233491 to 233865 unknown, highiy similar to dihydroneopterin aldolase
SeqlD n° 363 LM- 1407.1 From 232662 to 233477 unknown, highiy similar to dihydropteroate synthases
SeqlD n° 364 LM- 1408.1 From 230840 to 231766 unknown, highiy similar to cysteine synthase
SeqlD n° 365 LM- 1410.1 From 229840 to 230724 Unknwon, conserved hypothetical protein
SeqlD n° 366 LM- 1411.1 From 229045 to 229824 Unknwon, conserved hypothetical protein
SeqlD n° 367 LM- 1413.1 From 226854 to 228929 unknown, highiy similar to cell division protein ftsH
SeqlD n° 368 LM- 1415.1 From 224562 to 226508 Unknown, fusion protein,
N-terminal part similar to B. subtilis YacA protein, C- terminal part similar to hypoxanthine-guanine phosphoribosyltransferase SeqlD n° 369 LM-1418.1 From 224027 to 224455 Unknown, polyribonucleotide nucleotidyltransferase domain présent
SeqlD n° 370 LM-1420.1 From 223490 to 223876 unknown, similar to B. subtilis DivIC protein
SeqlD n° 371 LM-1426.1 From 221370 to 222959 Unknown, conserved membrane-spanning protein
SeqlD n° 372 LM-1429.1 From 217800 to 221339 transcription-repair coupling factor
SeqlD n° 373 LM-1430.1 From 217125 to 217685 Unknwon, similar to peptidyl-tRNA hydrolase
SeqlD n° 374 LM-1432.1 From 216434 to 217018 Unknown
SeqlD n° 375 LM-1433.2 From 215721 to 216344 Unknown, similar to B. subtilis gênerai stress protein
SeqlD n° 376 LM- 434.2 From 214486 to 215427 Unknwon, similar to L- lactate dehydrogenase
SeqlD n° 377 LM-1435.1 From 213735 to 214409 Unknown SeqlD n° 378 LM-1436.1 From 213336 to 213668 Unknown, conserved hypothetical protein
SeqlD n° 379 LM-1437.1 From 212824 to 213285 Unknown, hypothetical lipoprotein
SeqlD n° 380 LM-1438.1 From 212366 to 212689 Unknwon SeqlD n° 381 LM-1439.1 From 211425 to 212294 phospholipase C SeqlD n° 382 LM-1442.1 From 209470 to 211389 actin-assembly inducing protein precursor
SeqlD n° 383 LM-1444.1 From 207739 to 209271 Zinc metalloproteinase precursor
SeqlD n° 384 LM-1445.1 From 205819 to 207408 listeriolysin O precursor SeqlD n° 385 LM-1446.1 From 204624 to 205577 phosphatidylinositol- specific phospholipase c
SeqlD n° 386 LM-1447.1 From 203640 to 204353 listeriolysin positive regulatory protein
SeqlD n° 387 LM-1448.1 From 202641 to 203597 phosphoribosyl pyrophosphate synthetase
SeqlD n° 388 LM-1449.3 From 201217 to 202590 unknown, highiy similar to
UDP-N-acetylglucosamine pyrophosphorylase
SeqlD n° 389 LM-1451.1 From 1891236 to 1891880 Unknown, weakiy similar to thiamin pyrophosphokinase
SeqlD n° 390 LM-1453.1 From 1891944 to 1892600 Unknown, similar to ribulose-5-phosphate 3- epimerase
SeqlD n° 391 LM-1454.1 From 1892603 to 1893478 Unknown, similar to unknown proteins
SeqlD n° 392 LM-1457.1 From 1893497 to 1895464 Unknown, similar to putative serine/threonine- specific protein kinase
SeqlD n° 393 LM-1458.1 From 1895461 to 1896219 Unknown, similar to putative phosphoprotein phosphatase
SeqlD n° 394 LM-1459.1 From 1896242 to 1897576 Unknown, similar to RNA- binding Sun protein SeqlD n° 395 LM-1461.1 From 1897577 to 1898515 Unknown, similar to methionyl-tRNA formyltransferase
SeqlD n° 396 LM-1463.1 From 1898529 to 1900922 unknown, similar to primosomal réplication factor Y
SeqlD n° 397 LM-1465.1 From 1900927 to 1902126 unknown, similar to pantothenate metabolism flavoprotein homolog
SeqlD n° 398 LM-1466.2 From 1902484 to 1903101 unknown, similar to guanylate kinases SeqlD n° 399 LM-1467.2 From 1903120 to 1903995 unknown, simlar to conserved hypothetical protein
SeqlD n° 400 LM-1468.1 From 1904152 to 1905864 unknown, similar to fibronectin binding proteins SeqlD n° 401 LM-1469.1 From 1905952 to 1906551 unknown, similar to conserved hypotheticl proteins
SeqlD n° 402 LM-1470.1 From 1906592 to 1907221 unknown, highiy similar to orotate phosphoribosyltransferases
SeqlD n° 403 LM-1471.1 From 1907218 to 1907919 unknown, highiy similar to orotidine 5 -phosphate decarboxylases
SeqlD n° 404 LM-1472.1 From 1907916 to 1908830 unknown, highiy similar to dihydroorotase dehydrogenase
SeqlD n° 405 LM-1473.1 From 1908827 to 1909591 unknown, highiy similar to dihydroorotate dehydrogenase (électron transfer subunit)
SeqlD n° 406 LM-1474.1 From 1909614 to 1912826 unknown, highiy similar to carbamoyl-phosphate synthetase (catalytic subunit)
SeqlD n° 407 LM-1476.1 From 1912819 to 1913910 unknown, highiy similar to carbamoyl-phosphate synthetase (glutaminase subunit)
SeqlD n° 408 LM-1477.1 From 1913907 to 1915187 unknown, highiy similar to dihydroorotase SeqlD n° 409 LM-1478.1 From 1915175 to 1916086 unknown, highiy similar to aspartate carbamoyltransferase
SeqlD n° 410 LM-1480.1 From 1916166 to 1917452 unknown, highiy similar to uracil permease SeqlD n° 411 LM-1481.2 From 1917581 to 1918132 unknown, highiy similar to pyrimidine operon regulatory protein
SeqlD n° 412 LM-1482.2 From 1918363 to 1918593 unknown SeqlD n° 413 LM-1483.2 From 645397 to 645858 Unknown, similar to transcription regulator
MarR family
SeqlD n° 414 LM-1484.1 From 645878 to 647590 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 415 LM-1486.1 From 647587 to 649404 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 416 LM-1487.1 From 649520 to 649819 Unknown, similar to E. coli phage shock protein E
SeqlD n° 417 LM-1491.1 From 651794 to 652420 Unknown, similar to acyl- carrier protein phosphodiesterase and
NAD(P)H dehydrogenase
SeqlD n° 418 LM-1492.1 From 652589 to 653044 Unknown, similar to transcription regulator
MarR family
SeqlD n° 419 LM-1493.1 From 653041 to 653982 Unknown, similar to oxidoreductase
SeqlD n° 420 LM-1494.1 From 654078 to 654611 unknown, conserved hypothetical protein
SeqlD n° 421 LM-1495.1 From 654608 to 654850 Unknown
SeqlD n° 422 LM-1498.1 From 654957 to 656708 Unknown, C-terminal domain similar to glycerophosphoryl diester phosphodiesterase
SeqlD n° 423 LM-1499.1 From 656749 to 657243 Unknown SeqlD n° 424 LM-15.1 From 2720497 to 2721489 Unknown, similar to
Phosphotriesterase
SeqlD n° 425 LM-1500.1 From 657323 to 658465 Unknown, similar to protein kinase
SeqlD n° 426 LM-1501.1 From 658572 to 659027 Unknown SeqlD n° 427 LM-1502.1 From 659083 to 659475 Unknown SeqlD n° 428 LM-1504.1 From 659572 to 660312 Unknown, conserved hypothetical protein
SeqlD n° 429 LM-1506.1 From 660398 to 660676 Unknown, hypothetical SeqlD n° 430 LM-1507.1 From 660692 to 660958 Unknown SeqlD n° 431 LM-1508.1 From 660996 to 661439 Unknown, similar to unknown proteins
SeqlD n° 432 LM-1510.3 From 661470 to 662171 Unknown SeqlD n° 433 LM-1511.3 From 662257 to 663936 Unknown, similar to unknown protein
SeqlD n° 434 LM-1516.1 From 669423 to 669950 Unknown SeqlD n° 435 LM-1518.1 From 670309 to 672339 Unknown, similar to transcription antiterminator
BglG family SeqlD n° 436 LM-1519.1 From 672341 to 672793 Unknown, similar to PTS system, fructose-specific
MA component
SeqlD n° 437 LM-1520.1 From 672794 to 673855 Unknown, similar to PTS system, fructose-specific
IIC component
SeqlD n° 438 LM-1521.1 From 673870 to 674178 Unknown, similar to PTS system, fructose-specific
IIB component
SeqlD n° 439 LM-1523.1 From 674205 to 675473 Unknown, similar to an E. coli putative tagatose 6- phosphate kinase
SeqlD n° 440 LM-1524.1 From 675563 to 676267 Unknown SeqlD n° 441 LM-1525.1 From 676372 to 676788 Unknown, similar to unknown proteins
SeqlD n° 442 LM-1527.1 From 676802 to 677395 Unknown, weakiy similar to methyltransferase
SeqlD n° 443 LM-1528.1 From 678494 to 679123 Unknown SeqlD n° 444 LM-1529.1 From 679763 to 680296 Unknown, similar to a transcription regulator
(surface protein PAg négative regulator par)
SeqlD n° 445 LM-153.1 From 1216820 to 1218178 Unknown, similar to cobyrinic acid a,c-diamide synthase
SeqlD n° 446 LM-1530.1 From 680360 to 681277 Unknown, similar to oxidoreductase
SeqlD n° 447 LM-1532.1 From 681543 to 683423 Unknown, similar to heavy metal-transporting ATPase
SeqlD n° 448 LM-1533.1 From 683519 to 684247 Unknown
SeqlD n° 449 LM-1535.1 From 684390 to 685040 Unknown, similar to putative transaldolase
SeqlD n° 450 LM-1536.3 From 685083 to 686903 Unknown, similar to conserved hypothetical proteins
SeqlD n° 451 LM-1537.2 From 468169 to 469437 Unknown, weakiy similar to a module of peptide synthetase
SeqlD n° 452 LM-1538.1 From 467519 to 468136 unknown
SeqlD n° 453 LM-154.1 From 1215993 to 1216487 unknown
SeqlD n° 454 LM-1540.1 From 466517 to 467362 Unknown, conserved hypothetical protein
SeqlD n° 455 LM-1541.1 From 465934 to 466419 Unknown, similar to unknown proteins
SeqlD n° 456 LM-1545.1 From 459681 to 465722 Unknown, putative peptidoglycan linked protein (LPXTG motif)
SeqlD n° 457 LM-1547.1 From 457021 to 458913 Internalin B SeqlD n° 458 LM-1549.1 From 454534 to 456936 Internalin A SeqlD n° 459 LM-155.1 From 1215128 to 1215679 Unknown, similar to transcriptional regulator SeqlD n° 460 LM-1551.1 From 453107 to 453853 Unknown, similar to oxidoreductase
SeqlD n° 461 LM-1553.1 From 452524 to 453093 Unknown, similar to acetyltransferase
SeqlD n° 462 LM-1554.1 From 451531 to 452406 Unknown, similar to transcriptional regulator (LysR family)
SeqlD n° 463 LM-1557.1 From 448926 to 451508 Unknown, similar to sugar hydrolase
SeqlD n° 464 LM-1558.1 From 447804 to 448910 Unknown, similar to PTS fructose-specific enzyme IIC component
SeqlD n° 465 LM-156.1 From 1213636 to 1215087 unknown SeqlD n° 466 LM-1560.1 From 447471 to 447791 Unknown, similar to PTS fructose-specific enzyme MB component
SeqlD n° 467 LM-1561.1 From 447010 to 447474 Unknown, similar to PTS fructose-specific enzyme IIA component
SeqlD n° 468 LM-1564.1 From 445049 to 447010 Unknown, similar to transcription antiterminator BglG family
SeqlD n° 469 LM-1566.1 From 444031 to 444888 Unknown, similar to
Staphylococcus xylosus glucose uptake protein
SeqlD n° 470 LM-1568.1 From 443303 to 443851 Unknown, similar to RNA polymérase ECF-type sigma factor
SeqlD n° 471 LM-1569.1 From 442869 to 443300 Unknown, similar to unknown protein
SeqlD n° 472 LM-157.1 From 1212983 to 1213426 similar to ethanolamine utilization protein EutQ
SeqlD n° 473 LM-1570.1 From 441622 to 442872 Unknown, similar to rod shape-determining protein RodA
SeqlD n° 474 LM-1571.1 From 440758 to 441570 U n known , conse rved hypothetical protein
SeqlD n° 475 LM-1572.1 From 440056 to 440610 Unknown, similar to unknown protein
SeqlD n° 476 LM-1573.1 From 439677 to 439976 Unknown SeqlD n° 477 LM-1574.1 From 439214 to 439624 Unknown SeqlD n° 478 LM-1575.2 From 437482 to 438882 Unknown, similar to endo-
1 ,4-beta-xylanase
SeqlD n° 479 LM-1577.4 From 436379 to 437188 Unknown, conserved membrane protein
SeqlD n° 480 LM-1578.2 From 977051 to 978856 unknown, similar to heat shock protein HtpG
SeqlD n° 481 LM-1579.1 From 976065 to 977039 Unknown SeqlD n° 482 LM-158.1 From 1211869 to 1212990 Unknown, similar to ethanolamine utilization protein EutH - Escherichia coli SeqlD n° 483 LM-1580.1 From 975071 to 976033 Unknown
SeqlD n° 484 LM-1581.1 From 974229 to 974975 Unknown
SeqlD n° 485 LM-1582.1 From 973758 to 974216 unknown, similar to protein- tyrosine-phosphatase
SeqlD n° 486 LM-1583.1 From 972719 to 973459 unknown, similar to
Nitroflavin-reductase
SeqlD n° 487 LM-1584.1 From 972197 to 972706 Unknown, similar to B. subtilis CspR protein, rRNA methylase homolog
SeqlD n° 488 LM-1585.1 From 971037 to 972176 unknown, similar to B. subtilis YhbA protein
SeqlD n° 489 LM-1586.1 From 969549 to 970496 unknown, similar to B. subtilis YkcC protein
SeqlD n° 490 LM-1587.1 From 968819 to 969424 unknown, conserved hypothetical protein
SeqlD n° 491 LM-1589.1 From 967784 to 968779 unknown, similar to lipoate protein ligase A
SeqlD n° 492 LM-159.1 From 1211308 to 1211853 Unknown, similar to
Salmonella enterica PduT protein
SeqlD n° 493 LM-1590.1 From 967029 to 967760 unknown, conserved hypothetical protein, similar to B. subtilis Yhfl protein
SeqlD n° 494 LM-1592.1 From 966245 to 966913 unknown, similar to sortase SeqlD n° 495 LM-1593.1 From 965513 to 966136 unknown, similar to 3- methyladenine DNA glycosylase
SeqlD n° 496 LM-1594.1 From 963294 to 965255 unknown, hypothetical transmembrane protein
SeqlD n° 497 LM-1596.1 From 962607 to 963263 unknown, similar to transcription regulator
(TetR/AcrR family)
SeqlD n° 498 LM-1597.1 From 961469 to 962584 unknown, putative membrane protein
SeqlD n° 499 LM-16.1 From 2721559 to 2722857 Unknown, similar to membrane proteins
SeqlD n° 500 LM-160.1 From 1211046 to 1211315 Unknown, similar to carbon dioxide concentrating mechanism protein
SeqlD n° 501 LM-1600.1 From 960748 to 961221 Unknown, similar to ABC transporter, ATP-binding protein (N-terminal part)
SeqlD n° 502 LM-1602.1 From 959711 to 960631 unknown, similar to pantothenate kinase
SeqlD n° 503 LM-1603.1 From 958783 to 959622 unknown, similar to B. subtilis YcgQ protein
SeqlD n° 504 LM-1605.1 From 957724 to 958764 unknown, similar to B. subtilis YcgR protein SeqlD n° 505 LM-1606.1 From 956031 to 957602 unknown, similar to ABC transporter ATP-binding protein (antibiotic résistance)
SeqlD n° 506 LM-1607.1 From 953842 to 955740 Unknown, similar to transcription antiterminator
BglG family
SeqlD n° 507 LM-1608.1 From 952318 to 953769 Unknown, similar to beta- glucosidase
SeqlD n° 508 LM-1609.1 From 951956 to 952306 Unknown, similar to phosphotransferase system enzyme IIA
SeqlD n° 509 LM-161.1 From 1210459 to 1211031 unknown SeqlD n° 510 LM-1610.1 From 950673 to 951941 Unknown, similar to phosphotransferase system enzyme IIC
SeqlD n° 511 LM-1611.1 From 950363 to 950668 Unknown, similar to PTS system, IIB component SeqlD n° 512 LM-1613.1 From 948754 to 950220 Unknown, similar to succinate semialdehyde dehydrogenase
SeqlD n° 513 LM-1614.1 From 947718 to 948530 unknown, similar to transporters (formate)
SeqlD n° 514 LM-1615.1 From 947101 to 947604 Unknown SeqlD n° 515 LM-1617.1 From 945915 to 947018 Unknown SeqlD n° 516 LM-1618.1 From 945520 to 945912 Unknown, similar to transcription regulator,
GntR family
SeqlD n° 517 LM-1619.1 From 944243 to 945379 unknown, similar to membrane proteins SeqlD n° 518 LM-162.1 From 1209821 to 1210462 Unknown, similar io
Salmonella enterica PduL protein
SeqlD n° 519 LM-1620.1 From 943526 to 944200 unknown, similar to phosphoglycerate mutase SeqlD n° 520 LM-1622.1 From 942148 to 943497 Unknown, similar to glutathione Reductase
SeqlD n° 521 LM-1623.1 From 941615 to 942112 unknown SeqlD n° 522 LM-1624.2 From 940810 to 941484 Unknown SeqlD n° 523 LM-1625.2 From 469575 to 470078 Unknown SeqlD n° 524 LM-1626.1 From 470122 to 472158 Unknown, similar to penicillin-binding protein
(D-alanyl-D-alanine carboxypeptidase)
SeqlD n° 525 LM-1627.1 From 472330 to 472644 Unknown SeqlD n° 526 LM-1629.1 From 472781 to 473710 Unknown, similar to B. subtilis transcription regulator LytR
SeqlD n° 527 LM-163.1 From 1209053 to 1209808 unknown, similar to cobalamin adenosyl transferase SeqlD n° 528 LM-1631.1 From 473936 to 476716 Unknown, conserved hypothetical protein SeqlD n° 529 LM-1632.1 From 476960 to 478447 Unknown, similar to transcription regulator SeqlD n° 530 LM-1634.1 From 478721 to 479710 Unknown, similar to penicillin acylase and to conjugated bile acid hydrolase
SeqlD n° 531 LM-1635.1 From 479765 to 481153 Unknown, similar to glutamate decarboxylase
SeqlD n° 532 LM-1636.1 From 481250 to 482701 Unknown, similar to amino acid antiporter
SeqlD n° 533 LM-1637.1 From 483166 to 483891 Unknown SeqlD n° 534 LM-1638.1 From 483934 to 484599 Unknown, similar to unknown proteins
SeqlD n° 535 LM-1639.1 From 484620 to 485375 Unknown SeqlD n° 536 LM-164.1 From 1208603 to 1208887 unknown, similar to putative carboxysome structural protein
SeqlD n° 537 LM-1644.1 From 485493 to 487652 Unknown, similar to unknown proteins
SeqlD n° 538 LM-1645.1 From 487649 to 488797 Unknown, conserved hypothetical proteins
Seq I D n ° 539 LM- 1646.1 From 488802 to 489749 Unknown, conserved hypothetical protein similar to B. subtilis YeaC
SeqlD n° 540 LM-1647.1 From 489905 to 491500 Unknown, similar to unknown proteins
SeqlD n° 541 LM-1650.1 From 491634 to 492917 Unknown, similar to permeases
SeqlD n° 542 LM-1652.1 From 492918 to 494018 Unknown, similar to unknown proteins
SeqlD n° 543 LM-1653.1 From 494011 to 495561 Unknown, similar to hydantoinase
SeqlD n° 544 LM-1655.1 From 496381 to 497919 Unknown, similar to transcription regulator (VirR from Streptococcus pyogenes)
SeqlD n° 545 LM-1656.1 From 498171 to 500240 Unknown, putative membrane associated lipoprotein
SeqlD n° 546 LM-1658.1 From 500306 to 500779 Unknown SeqlD n° 547 LM-1659.3 From 500799 to 501284 Unknown SeqlD n° 548 LM-166.1 From 1207111 to 1208571 unknown, similar to acetaldehyde dehydrogenase / alcohol dehydrogenase
SeqlD n° 549 LM-1661.4 From 2632907 to 2633761 Unknown, similar to fructose- 1 ,6-bisphosphate aldolase SeqlD n° 550 LM-1662.2 From 2631303 to 2632586 Unknwon, weakiy similar to human N- acetylglucosaminyl- phosphatidylinositol biosynthetic protein
SeqlD n° 551 LM-1663.1 From 2630285 to 2631310 Unknown, similar to galactosyltransferase SeqlD n° 552 LM-1664.1 From 2629208 to 2630278 Unknown, conserved hypothetical protein SeqlD n° 553 LM-1665.1 From 2627816 to 2629087 unknown, highiy similar to
UDP-N-acetylglucosamine
1-carboxyvinyltransferase
SeqlD n° 554 LM-1666.2 From 2626442 to 2627713 unknown, highiy similar to transcription terminator factor rho
SeqlD n° 555 LM-1668.2 From 2625414 to 2626361 Unknwon, similar to glycosyl transferases SeqlD n° 556 LM-1669.1 From 2624980 to 2625417 wall teichoic acid glycosylation protein GtcA SeqlD n° 557 LM-167.1 From 1206590 to 1207111 Unknown, similar to putative carboxysome structural protein
SeqlD n° 558 LM-1671.1 From 2623125 to 2624411 unknown, highiy similar to homoserine dehydrogenase
SeqlD n° 559 LM-1674.1 From 2622067 to 2623122 unknown, highiy similar to thréonine synthase SeqlD n° 560 LM-1676.1 From 2621201 to 2622067 unknown, highiy similar to homoserine kinase SeqlD n° 561 LM-1678.1 From 2620514 to 2621089 Unknwon, similar to thymidine kinase SeqlD n° 562 LM-1679.1 From 2619415 to 2620491 unknown, highiy similar to peptide chain release factor 1
SeqlD n° 563 LM-168.1 From 1205922 to 1206575 Unknown, similar to putative carboxysome structural protein (eutL)
SeqlD n° 564 LM-1681.1 From 2618577 to 2619428 Unknown, similar to protoporphyrinogen oxidase
SeqlD n° 565 LM-1682.1 From 2617239 to 2618276 Unknown, similar to yeast translation initiation protein
SeqlD n° 566 LM-1683.1 From 2616835 to 2617242 Unknown, similar to phosphatases SeqlD n° 567 LM-1684.1 From 2615458 to 2616699 unknown, highiy similar to glycine hydroxymethyltransferase
SeqlD n° 568 LM-1685.1 From 2614692 to 2615321 unknown, highiy similar to uracil phosphoribosyltransferase SeqlD n° 569 LM-1686.1 From 2613435 to 2614574 Unknwon, similar to UDP- N-acetylglucosamine 2- epimerase
SeqlD n° 570 LM-1691.1 From 2612205 to 2612603 unknown, highiy similar to
ATP synthase subunit i
SeqlD n° 571 LM-1693.1 From 2611482 to 2612198 unknown, highiy similar to
H+-transporting ATP synthase chain a
SeqlD n° 572 LM-1694.1 From 2610592 to 2611104 unknown, highiy similar to
H+-transporting ATP synthase chain b
SeqlD n° 573 LM-1695.1 From 2610056 to 2610595 unknown, highiy similar to
H+-transporting ATP synthase chain delta
SeqlD n° 574 LM-1697.1 From 2608515 to 2610029 unknown, highiy similar to
H+-transporting ATP synthase chain alpha
SeqlD n° 575 LM-1698.1 From 2607605 to 2608477 unknown, highiy similar to
H+-transporting ATP synthase chain gamma
SeqlD n° 576 LM-170.1 From 1205018 to 1205899 Unknown, similar to ethanolamine ammonia- lyase, light chain
SeqlD n° 577 LM-1700.1 From 2606123 to 2607544 unknown, highiy similar to
H+-transporting ATP synthase chain beta
SeqlD n° 578 LM-1701.1 From 2605697 to 2606101 unknown, highiy similar to
H+-transporting ATP synthase chain epsilon
SeqlD n° 579 LM-1702.1 From 2605337 to 2605573 Unknown, similar to B. subtilis YwzB protein
SeqlD n° 580 LM-1704.1 From 2603863 to 2605155 unknown, UDP-N- acetylglucosamine 1- carboxyvinyltransferase
SeqlD n° 581 LM-1705.2 From 2602705 to 2603700 unknown, similar to MreB- like protein
SeqlD n° 582 LM-1706.2 From 521863 to 522591 Unknown
SeqlD n° 583 LM-1707.1 From 522628 to 523521 Unknown, similar to transcriptional regulator
(LysR family)
SeqlD n° 584 LM-1708.1 From 523719 to 525713 Unknown, similar to
NADH.TIavin oxidoreductase
SeqlD n° 585 LM-171.1 From 1203634 to 1204998 Unknown, similar to ethanolamine ammonia- lyase, heavy chain
SeqlD n° 586 LM-1710.1 From 525813 to 526688 Unknown, similar to shikimate 5- dehydrogenase
SeqlD n° 587 LM-1712.1 From 526754 to 527512 Unknown, similar to 3- dehydroquinate dehydratase SeqlD n° 588 LM-1713.1 From 527580 to 528488 Unknown, similar to transcriptional regulator
(LysR family)
SeqlD n° 589 LM- 1714.1 From 528563 to 530323 Unknown, similar to acylase
SeqlD n° 590 LM- 1715.1 From 530515 to 531108 Unknown, weakiy similar to esterase
SeqlD n° 591 LM- 1716.1 From 531294 to 532253 Unknown, similar to transmembrane protein
SeqlD n° 592 LM- 1717.1 From 532328 to 532552 Unknown, similar to B. subtilis YnzC protein
SeqlD n° 593 LM- 1718.2 From 532603 to 534111 Unknown, similar to sugar transferase
SeqlD n° 594 LM- 1719.1 From 534307 to 534756 Unknown, similar to ribose
5-phosphate iso erase
SeqlD n° 595 LM- 1721.1 From 534753 to 535421 Unknown, similar to ribulose-5-phosphate 3 epimerase
SeqlD n° 596 LM-1722.1 From 535428 to 536078 Unknown, similar to transaldolase
SeqlD n° 597 LM-1723.1 From 536187 to 538247 Unknown, similar to transcription antiterminator
BglG family
SeqlD n° 598 LM-1724.1 From 538251 to 538853 Unknown, similar to putative sugar-phosphate isomerase
SeqlD n° 599 LM-1725.1 From 538881 to 539348 Unknown, similar to PTS fructose-specific enzyme
IIA component
SeqlD n° 600 LM-1726.1 From 539365 to 539763 unknown SeqlD n° 601 LM-1727.1 From 539774 to 540424 Unknown, similar to ribulose-5-phosphate 3- epimerase
SeqlD n° 602 LM-1728.1 From 540421 to 541467 Unknown, similar to polyol
(sorbitol) dehydrogenase SeqlD n° 603 LM-1729.1 From 541483 to 541776 Unknown, similar to PTS system, Galactitol-specific
IIB component
SeqlD n° 604 LM-173.1 From 1202171 to 1203592 Unknown, similar to ethanolamine utilization protein EutA (putative chaperonin)
SeqlD n° 605 LM-1731.1 From 541791 to 543062 Unknown, similar to PTS system, Galactitol-specific
IIC component
SeqlD n° 606 LM-1733.1 From 543202 to 544137 Unknown, similar to phosphoribosyl pyrophosphate synthetase
SeqlD n° 607 LM-1734.1 From 544923 to 545501 Unknown SeqlD n° 608 LM-1735.1 From 545609 to 546289 Unknown, conserved hypothetical protein
SeqlD n° 609 LM-1736.1 From 546314 to 546673 Unknown SeqlD n° 610 LM-1737.1 From 546680 to 547138 Unknown, weakiy similar to transcription regulator
SeqlD n° 611 LM-174.1 From 1200625 to 1202082 unknown, similar to sensory transduction histidine kinase
SeqlD n° 612 LM-1740.1 From 549438 to 549869 Unknown, conserved hypothetical protein
SeqlD n° 613 LM-1741.1 From 549916 to 551346 Unknown, similar to
Bacillus anthracis encapsulation protein
CapA
SeqlD n° 614 LM-1742.2 From 551467 to 552282 Unknown, similar to phosphoglycerate mutase
SeqlD n° 615 LM-175.1 From 1200051 to 1200632 unknown, similar to similar to two-component response regulator
SeqlD n° 616 LM-1755.2 From 2678302 to 2679330 Unknown, similar to ATP binding proteins
SeqlD n° 617 LM-1757.1 From 2681167 to 2682018 Unknown, similar to oxidoreductase, aldo/keto reductase family
SeqlD n° 618 LM-1758.1 From 2682040 to 2682462 Unknown, similar to transcription regulators
(MerR family)
SeqlD n° 619 LM-1759.1 From 2682584 to 2682943 Unknown
SeqlD n° 620 LM-176.1 From 1198637 to 1199776 unknown, similar to
NADPH-dependent butanol dehydrogenase
SeqlD n° 621 LM-1760.1 From 2683187 to 2684056 Unknown, similar to unknown proteins
SeqlD n° 622 LM-1761.1 From 2684251 to 2684643 ribosomal protein S9
SeqlD n° 623 LM-1762.1 From 2684665 to 2685102 ribosomal protein L13
SeqlD n° 624 LM-1763.1 From 2685297 to 2686043 unknown, highiy similar to pseudouridylate synthase I
SeqlD n° 625 LM-1766.1 From 2686049 to 2686846 Unknown, highiy similar to
B. subtilis YbaF protein
SeqlD n° 626 LM-1769.1 From 2686849 to 2687715 Uknown similar to ABC transporter (ATP-binding protein)
SeqlD n° 627 LM-1771.1 From 2687691 to 2688530 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 628 LM-1772.1 From 2688661 to 2689323 Unknown, conserved hypothetical protein
SeqlD n° 629 LM-1773.1 From 2689442 to 2690332 Unknown
SeqlD n° 630 LM-1774.1 From 2690371 to 2691465 Unknown
SeqlD n° 631 LM-1775.2 From 2691673 to 2692080 ribosomal protein L17
SeqlD n° 632 LM-1776.3 From 1441313 to 1441540 Unknown
SeqlD n° 633 LM-1777.3 From 1441581 to 1442114 Unknown, modulâtes DNA topology
SeqlD nc ' 634 LM-1779.1 From 1443873 to 1445042 Unknown, similar to Acetyl-
CoA:acetyltransferase SeqlD n° 635 LM-1780.2 From 1445192 to 1446358 Unknown, similar to hydroxy-3-methylglutaryl coenzyme A synthase
SeqlD n° 636 LM-1781.2 From 1446383 to 1446982 Unknown SeqlD n° 637 LM-1782.1 From 1447052 to 1448296 Unknown, highiy similar to
B. subtilis YxiO protein
SeqlD n° 638 LM-1783.1 From 1448315 to 1449583 Unknown, weakiy similar to pyrophosphatase
SeqlD n° 639 LM-1784.1 From 1449647 to 1450684 Unknown, conserved hypothetical protein
SeqlD n° 640 LM-1785.1 From 1450701 to 1451597 Unknown, weakiy similar to
UDP-N-acetylglucosaminyl-
3-enolpyruvate reductase
SeqlD n° 641 LM-1787.1 From 1451813 to 1452799 Unknown, similar to glycine betaine/carnitine/choline
ABC transporter
SeqlD n° 642 LM-1789.1 From 1452796 to 1454310 Unknown, similar to glycine betaine/carnitine/choline
ABC transporter
(membrane protein)
SeqlD n° 643 LM-179.1 From 1197172 to 1198047 unknown, similar to
Salmonella enterica PduX protein
SeqlD n° 644 LM-1790.1 From 1454347 to 1455177 Unknown SeqlD n° 645 LM-1791.2 From 1455316 to 1456662 Unknown, similar to métal ion transport proteins
SeqlD n° 646 LM-1794.2 From 1456775 to 1457446 Unknown, similar to betaine/carnitine/choline
ABC transporter
(membrane p)
SeqlD n° 647 LM-1795.1 From 1457461 to 1458387 Unknown, similar to glycine betaine/carnitine/choline
ABC transporter (osmoprotectant-binding protein)
SeqlD n° 648 LM-1796.1 From 1458389 to 1459045 Unknown, similar to glycine betaine/carnitine/choline ABC transporter (membrane protein)
SeqlD n° 649 LM-1798.1 From 1459049 to 1460242 Unknown, similar to glycine betaine/carnitine/choline ABC transporter (ATP- binding protein)
SeqlD n° 650 LM-1799.1 From 1460534 to 1461094 Unknown, similar to unknown proteins SeqlD n° 651 LM-18.1 From 2722884 to 2723156 Unknwon, similar to hypothetical PTS enzyme IIB component
SeqlD n° 652 LM-1800.2 From 1461343 to 1461726 Unknown, similar to unknown proteins SeqlD n° 653 LM-1801.2 From 1461866 to 1463467 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 654 LM-1802.1 From 1463506 to 1464156 Unknown
SeqlD n° 655 LM-1803.1 From 1464209 to 1465549 Unknown, similar to glutathione reductase
SeqlD n° 656 LM-1805.1 From 1465638 to 1467305 Unknown, similar to unknown proteins
SeqlD n° 657 LM-1806.1 From 1467325 to 1468206 Unknown, similar to dihydrodipicolinate synthase
SeqlD n° 658 LM-1807.1 From 1468221 to 1469432 Unknown, similar to aspartokinase I (alpha and beta subunits)
SeqlD n° 659 LM-1809.1 From 1469444 to 1470487 Unknown, similar to aspartate-semialdehyde dehydrogenase
SeqlD n° 660 LM-1810.1 From 1470685 to 1472850 unknown, similar to penicillin-binding protein
Seq I D n ° 661 LM- 1811.1 From 1472980 to 1473588 superoxide dismutase
SeqlD n° 662 LM-1813.1 From 1473889 to 1474689 unknown, similar to unknown proteins
SeqlD n° 663 LM-1814.1 From 1474802 to 1475908 Unknown, similar to putative peptidoglycan acetylation protein
SeqlD n° 664 LM-1815.1 From 1475944 to 1476651 Unknown, similar to transport proteins
SeqlD n° 665 LM-1816.1 From 1476642 to 1476968 Unknown
SeqlD n° 666 LM-1817.1 From 1477050 to 1477934 Unknown, similar to protein sécrétion PrsA (post- translocation molecular chaperone)
SeqlD n° 667 LM-1818.1 From 1478071 to 1478496 transcriptional regulator
ZurR (ferrie uptake régulation)
SeqlD n° 668 LM-182.1 From 1194890 to 1196083 Unknown, similar to acétate kinase
SeqlD n° 669 LM-1820.1 From 1478477 to 1479355 métal transport protein SeqlD n° 670 LM-1821.1 From 1479330 to 1480103 ABC transporter SeqlD n° 671 LM-1823.1 From 1480246 to 1481172 Unknown, conserved hypothetical protein
SeqlD n° 672 LM-1825.1 From 1481188 to 1482081 unknown, similar to endonuclease IV
SeqlD n° 673 LM-1826.1 From 1482096 to 1483403 Unknown, similar to ATP- dependent RNA helicase,
DEAD-box family (deaD)
SeqlD n° 674 LM-1827.1 From 1483549 to 1484544 Unknown, similar to E. coli
LytB protein SeqlD n° 675 LM-1829.1 From 1484590 to 1485711 Unknown, conserved hypothetical protein SeqlD n° 676 LM-183.1 From 1194120 to 1194824 Unknown, similar to glycerol uptake facilitator protein
SeqlD n° 677 LM-1830.1 From 1485708 to 1486412 Unknown, conserved hypothetical protein SeqlD n° 678 LM-1833.2 From 1486480 to 1487604 RNA polymérase sigma factor RpoD SeqlD n° 679 LM-1834.2 From 289562 to 290521 Unknown, similar to other proteins SeqlD n° 680 LM-1835.1 From 289139 to 289555 Unknown, similar to transcriptional regulators SeqlD n° 681 LM-1836.1 From 287853 to 288992 Unknown, similar to succinyldiaminopimelate desuccinylase
SeqlD n° 682 LM-1838.1 From 286219 to 287718 internalin E SeqlD n° 683 LM-184.1 From 1192974 to 1194092 unknown, similar to
NADPH-dependent butanol dehydrogenase
SeqlD n° 684 LM-1840.1 From 284365 to 286011 internalin H SeqlD n° 685 LM-1842.1 From 282755 to 284227 internalin G SeqlD n° 686 LM-1843.1 From 281021 to 282481 Unknown, similar to phospho-beta-glucosidase
SeqlD n° 687 LM-1844.1 From 280410 to 280955 Unknown, similar to unknown proteins
SeqlD n° 688 LM-185.1 From 1191549 to 1192958 Unknown, similar to ethanolamine utilization protein EutE
SeqlD n° 689 LM-1852.1 From 276728 to 280333 RNA polymérase (beta subunit)
SeqlD n° 690 LM-1854.1 From 273003 to 276557 RNA polymérase (beta subunit)
SeqlD n° 691 LM-1856.2 From 271324 to 272502 Unknown, similar to unknown protein
SeqlD n° 692 LM-1858.1 From 269754 to 270257 Unknown, similar to unknown protein
SeqlD n° 693 LM-1859.2 From 269067 to 269729 Unknown SeqlD n° 694 LM-1860.2 From 1993372 to 1994637 Unknown, similar to unknown proteins
SeqlD n° 695 LM-1861.1 From 1991047 to 1993326 Unknown, similar to pyruvate formate-lyase
SeqlD n° 696 LM-1862.1 From 1989805 to 1990812 Unknown, similar to peptidase
SeqlD n° 697 LM-1863.1 From 1988159 to 1989802 Unknown, similar to malolactic enzyme (malate dehydrogenase)
SeqlD n° 698 LM-1864.1 From 1987365 to 1988048 Unknown, similar to unknown proteins SeqlD n° 699 LM-1865.1 From 1986342 to 1987346 Unknown, similar to unknown proteins SeqlD n° 700 LM-1866.1 From 1985218 to 1986345' Unknown, similar to unknown proteins (hypothetical sensory transduction histidine kinase)
SeqlD n° 701 LM-1867.1 From 1984056 to 1985192 Unknown, similar to unknown proteins
(hypothetical sensory transduction histidine kinase)
SeqlD n° 702 LM-1869.1 From 1982630 to 1983736 Unknown, similar to oxidoreductases
SeqlD n° 703 LM-1871.1 From 1981774 to 1982640 Unknown, similar to unknown proteins
SeqlD n° 704 LM-1872.1 From 1981299 to 1981634 Unknown, similar to unknown proteins
SeqlD n° 705 LM-1874.1 From 1980511 to 1981302 Unknown, similar to dihydrodipicolinate reductase
SeqlD n° 706 LM-1875.1 From 1980091 to 1980495 Unknown, similar to methylglyoxal synthase
SeqlD n° 707 LM-1876.1 From 1978895 to 1980076 Unknown, similar to tRNA
CCA-adding enzyme
SeqlD n° 708 LM-1877.1 From 1977928 to 1978905 Unknown, similar to transcriptional regulator and biotin acetyl-CoA- carboxylase synthetase
SeqlD n° 709 LM-1879.1 From 1977307 to 1977780 unknown; similar to thioredoxin
SeqlD n° 710 LM-188.1 From 1190547 to 1191542 Unknown, hyghiy similar to
Salmonella enterica PduO protein
SeqlD n° 711 LM-1880.1 From 1976307 to 1977140 unknown, similar to ketopantoate hydroxymethyltransferases
SeqlD n° 712 LM-1881.1 From 1975446 to 1976303 unknown, similar to panthotenate synthetases
SeqlD n° 713 LM-1882.1 From 1975059 to 1975442 unknown, similar to aspartate 1- decarboxylases
SeqlD n° 714 LM-1884.1 From 1972176 to 1974962 unknown, similar to ATP- dependent helicases
SeqlD n° 715 LM-1885.1 From 1971547 to 1972131 unknown, similar to hypothetical proteins
SeqlD n° 716 LM-1886.1 From 1970343 to 1971524 unknown, similar to aspartate aminotransferases
SeqlD n° 717 LM-1888.1 From 1969037 to 1970329 unknown, similar to sparaginyl-tRNA synthetases
SeqlD n° 718 LM-189.1 From 1190269 to 1190532 Unknown, similar to carbon dioxide concentrating mechanism protein SeqlD n° 719 LM-1891.1 From 1968169 to 1968888 unknown, similar to chromosome réplication initiation protein
SeqlD n° 720 LM-1893.1 From 1967499 to 1968158 unknown, probable endonuclease III (DNA repair)
SeqlD n° 721 LM-1894.1 From 1967015 to 1967506 unknown SeqlD n° 722 LM- 896.1 From 1964489 to 1966972 unknown, similar to penicillin-binding protein 2A
SeqlD n° 723 LM-1897.2 From 1963852 to 1964457 unknown, similar to DNA repair and homologous recombination protein
SeqlD n° 724 LM-1898.2 From 2208576 to 2210351 Unknown, similar to maltogenic amylase SeqlD n° 725 LM-19.1 From 2723159 to 2723593 Unknwon, similar to mannitol-specific PTS enzyme IIA component
SeqlD n° 726 LM-190.1 From 1189785 to 1190264 Unknown SeqlD n° 727 LM-1900.1 From 2207103 to 2208362 Unknown, similar to maltose/maltodextrin- binding protein
SeqlD n° 728 LM-1904.1 From 2205709 to 2207016 Unknown, similar to maltodextrin transport system permease SeqlD n° 729 LM-1905.1 From 2204857 to 2205708 Unknown, similar to maltodextrin transport system permease SeqlD n° 730 LM-1906.1 From 2203996 to 2204829 Unknown, similar to maltodextrose utilization protein MalA
SeqlD n° 731 LM-1907.1 From 2201719 to 2203980 Unknown, similar to maltosephosphorylase
SeqlD n° 732 LM- 908.1 From 2200723 to 2201544 Unknown, similar to unknown proteins
SeqlD n° 733 LM-1910.1 From 2199365 to 2200723 Unknown, similar to unknown proteins
SeqlD n° 734 LM-1911.1 From 2197763 to 2199115 Unknown, similar to phosphoglucomutase
SeqlD n° 735 LM-1912.1 From 2197267 to 2197728 Unknown, similar to unknown proteins
SeqlD n° 736 LM-1913.1 From 2196382 to 2197206 unknown SeqlD n° 737 LM-1915.1 From 2194399 to 2196339 Unknown, similar to ABC transporter (permease)
SeqlD n° 738 LM-1916.1 From 2193645 to 2194412 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 739 LM-1918.2 From 2192693 to 2193448 unknown, similar to unknown proteins SeqlD n° 740 LM-1919.2 From 2191525 to 2192256 Unknown, similar to FMN- containing NADPH-linked nitro/flavin reductase 1
81
SeqlD n° 741 LM-192.1 From 1188949 to 1189788 Unknown, similar to ethanolamine utilization protein EutJ SeqlD n° 742 LM-1920.1 From 2190489 to 2191445 Unknown, similar to mannnose-6 phospate isomelase
SeqlD n° 743 LM-1921.1 From 2189605 to 2190402 Unknown, similar to hydrolase SeqlD n° 744 LM-1922.1 From 2188430 to 2189563 Unknown, similar to N- acetylglucosamine-6- phosphate deacetylase
SeqlD n° 745 LM-1923.1 From 2187663 to 2188424 Unknown, similar to transcriptional regulator
(DeoR family)
SeqlD n° 746 LM-1924.1 From 2186557 to 2187411 Unknown, similar to unknown proteins
SeqlD n° 747 LM-1927.1 From 2184344 to 2186338 Unknown, similar to ferrous iron transport protein B
SeqlD n° 748 LM-1930.1 From 2182823 to 2183800 Unknown, similar to phosphotransacetylase
SeqlD n° 749 LM-1932.1 From 2182218 to 2182784 Unknown SeqlD n° 750 LM-1933.2 From 2181329 to 2182216 Unknown, similar to a protein required for pyridoxine synthesis
SeqlD n° 751 LM-1935.1 From 1610206 to 1611165 unknown, highiy similar to
6-phosphofructokinase SeqlD n° 752 LM-1936.1 From 1611449 to 1612405 unknown, highiy similar to acetyl CoA carboxylase
(alpha subunit)
SeqlD n° 753 LM-1938.1 From 1612395 to 1613279 unknown, highiy similar to acetyl-CoA carboxylase beta subunit
SeqlD n° 754 LM-1942.1 From 1613497 to 1616823 unknown, highiy similar to
DNA polymérase III (alpha subunit) DnaE
SeqlD n° 755 LM-1944.1 From 1616945 to 1617880 Unknown, similar to unknown proteins SeqlD n° 756 LM-1945.1 From 1617901 to 1619214 Unknown, similar to unknown proteins SeqlD n° 757 LM-1946.1 From 1619240 to 1619926 Unknown, similar to unknown proteins SeqlD n° 758 LM-1947.1 From 1620091 to 1621188 Unknown, similar to X-Pro dipeptidase SeqlD n° 759 LM-1948.1 From 1621230 to 1622342 Unknown, similar to alanine dehydrogenase SeqlD n° 760 LM-1949.1 From 1622583 to 1623047 Unknown, similar to unknown protein SeqlD n° 761 LM-195.1 From 1188293 to 1188928 unknown, similar to
Salmonella enterica PduL protein
SeqlD n° 762 LM-1950.1 From 1623099 to 1624292 unknown, highiy similar to acétate kinase 82
SeqlD n° 763 LM-1951.1 From 1624316 to 1625314 Unknown, weakiy similar to site spécifie DNA- methyltransferase
SeqlD n° 764 LM-1952.1 From 1625505 to 1626002 Unknown, similar to thiol peroxidases SeqlD n° 765 LM-1953.1 From 1626064 to 1626600 Unknown, similar to unknown proteins SeqlD n° 766 LM-1954.1 From 1626620 to 1627633 Unknown, similar to proteases SeqlD n° 767 LM-1955.1 From 1627812 to 1628615 Unknown, similar to unknown proteins SeqlD n° 768 LM-1957.1 From 1628647 to 1629591 unknown, highiy similar to orn/thine carbamoyltransferase
SeqlD n° 769 LM-1958.1 From 1629594 to 1630754 unknown, highiy similar to
N-acetylornithine aminotransferase
SeqlD n° 770 LM-1959.1 From 1630751 to 1631503 unknown, highiy similar to
N-acetylglutamate 5- phosphotransferase
SeqlD n° 771 LM-1960.1 From 1631516 to 1632712 unknown, highiy similar to ornithine acetyltransferase and amino-acid acetyltransferases
SeqlD n° 772 LM-1961.2 From 1632728 to 1633759 unknown, similar to N- acetylglutamate gamma- semialdehyde dehydrogenases
SeqlD n° 773 LM-1962.2 From 1633933 to 1635144 Unknown, similar to thiamin biosynthesis protein Thil SeqlD n° 774 LM-1964.1 From 1635146 to 1636285 Unknown, similar to iron- sulfur cofactor synthesis protein nifS
SeqlD n° 775 LM-1966.2 From 1636412 to 1638127 Unknown, similar to B. subtilis négative regulator of FtsZ ring formation
(EzrA)
SeqlD n° 776 LM-1968.2 From 1308351 to 1310318 unknown, highiy similar to
DNA gyrase-like protein
(subunit B)
SeqlD n° 777 LM-197.1 From 1187552 to 1187989 unknown, similar to
Salmonella enterica PduK protein
SeqlD n° 778 LM-1970.1 From 1310315 to 1312774 unknown, highiy similar to
DNA gyrase-like protein
(subunit A)
SeqlD n° 779 LM-1971.1 From 1312857 to 1313324 unknown, conserved hypothetical protein SeqlD n° 780 LM-1976.1 From 1317596 to 1319464 unknown; similar to acyltransferase (to B. subtilis YrhL protein) 83
SeqlD n° 781 LM-1978.1 From 1319677 to 1320375 unknown similar to glycerophosphodiester phosphodiesterase SeqlD n° 782 LM-1979.1 From 1320608 to 1322284 Unknown, similar to glycerol 3 phosphate dehydrogenase SeqlD n° 783 LM-198.1 From 1187192 to 1187539 unknown, similar to diol dehydratase-reactivating factor small chain SeqlD n° 784 LM-1980.1 From 1322410 to 1323327 Unknown, similar to tRNA isopentenylpyrophosphate transferase
SeqlD n° 785 LM-1981.1 From 1323450 to 1323683 Unknown, similar to host factor-1 protein SeqlD n° 786 LM-1983.1 From 1323794 to 1325017 unknown, conserved hypothetical protein similar to B. subtilis YnbA protein
SeqlD n° 787 LM-1984.1 From 1325010 to 1326236 unknown, similar to aluminum résistance protein and to B. subtilis
YnbB protein
SeqlD n° 788 LM-1985.1 From 1326440 to 1326808 Unknown, similar to glutamine synthetase repressor
SeqlD n° 789 LM-1986.1 From 1326879 to 1328213 unknown, highiy similar to glutamine synthetases SeqlD n° 790 LM-1988.1 From 1328357 to 1329652 Unknown, similar to arsenic efflux pump protein SeqlD n° 791 LM-1989.1 From 1329696 to 1330217 unknown, conserved hypothetical protein SeqlD n° 792 LM-1991.2 From 1330247 to 1330861 unknown, highiy similar to
SOS response regulator lexA, transcription repressor protein
SeqlD n° 793 LM-1992.2 From 1331018 to 1331347 unknown, similar to B. subtilis YneA protein
SeqlD n° 794 LM-1995.1 From 1331813 to 1333807 unknown, highiy similar to transketolase
SeqlD n° 795 LM-1996.1 From 1334028 to 1334267 unknown, highiy similar to B. subtilis YneF protein
SeqlD n° 796 LM-1997.1 From 1334318 to 1335160 unknown SeqlD n° 797 LM-1998.1 From 1335179 to 1335910 unknown, weakiy similar to arginine N- methyltransferases
SeqlD n° 798 LM-1999.1 From 1335932 to 1336438 unknown, similar to E. coli YbdM protein SeqlD n° 799 LM-200.1 From 1185375 to 1187195 diol dehydratase- reactivating factor large subunit
SeqlD n° 800 LM-2000.1 From 1336448 to 1337752 unknown, similar to E. coli YbdN protein SeqlD n° 801 LM-2001.1 From 1337742 to 1338947 unknown 84
SeqlD n° 802 LM-2002.1 From 1338925 to 1339299 Unknown
SeqlD n° 803 LM-2003.1 From 1339592 to 1340320 unknown, highiy similar to uridylate kinases
SeqlD n° 804 LM-2004.1 From 1340320 to 1340877 unknown, highiy similar to ribosome recycling factors
SeqlD n° 805 LM-2005.2 From 1341107 to 1341865 Unknown, similar to undecaprenyl diphosphate synthase
SeqlD n° 806 LM-2006.2 From 290593 to 291228 Unknown, similar to phosphoglycerate mutase
SeqlD n° 807 LM-2008.1 From 291312 to 293198 Unknown, similar to transporter
SeqlD n° 808 LM-2009.1 From 293212 to 293841 Unknown
SeqlD n° 809 LM-201.1 From 1184818 to 1185330 Unknown, similar to diol dehydrase (diol dehydratase) gamma subunit (pddC)
SeqlD n° 810 LM-2010.1 From 293845 to 295281 unknown, highiy similar to phospho-beta-glucosidase
SeqlD n° 811 LM-2011.1 From 295401 to 296213 Unknown, conserved hypothetical protein similar to B. subtilis YxeH protein
SeqlD n° 812 LM-2012.1 From 296349 to 296852 Unknown SeqlD n° 813 LM-2013.1 From 296889 to 297551 Unknown SeqlD n° 814 LM-2014.1 From 297810 to 298652 Unknown, C-terminal part similar to B. subtilis
ComEC protein
SeqlD n° 815 LM-2015.1 From 298669 to 299490 Unknown, conserved hypothetical protein
SeqlD n° 816 LM-2017.1 From 299607 to 300578 Unknown, similar to oxidoreductase
SeqlD n° 817 LM-2018.1 From 300617 to 301717 Unknown, similar to sugar ABC transporter, ATP- binding protein
SeqlD n° 818 LM-2019.1 From 302008 to 304140 Unknown, highiy similar to anaerobic ribonucleoside- triphosphate reductase
SeqlD n° 819 LM-202.1 From 1184142 to 1184801 Unknown, similar to diol dehydrase (diol dehydratase) gamma subunit
SeqlD n° 820 LM-2020.1 From 304133 to 304684 Unknown, highiy similar to anaerobic ribonucleotide reductase activator protein
SeqlD n° 821 LM-2022.1 From 304942 to 305613 Unknown SeqlD n° 822 LM-2023.1 From 305775 to 306554 Unknown, conserved hypothetical protein
SeqlD n° 823 LM-2024.1 From 306644 to 307306 Unknown, similar to ABC transporter permease protein SeqlD n° 824 LM-2026.1 From 307303 to 308319 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 825 LM-2027.2 From 308334 to 309155 Unknown, putative lipoprotein
SeqlD n° 826 LM-203.1 From 1182440 to 1184104 unknown, highiy similar to propanediol dehydratase, alpha subunit
SeqlD n° 827 LM-2035.1 From 2671624 to 2672511 Unknwon, similar to transcription regulator
TetR/AcrR family
SeqlD n° 828 LM-2037.1 From 2670042 to 2671523 Unknwon, similar to drug- export proteins
SeqlD n° 829 LM-2038.1 From 2669152 to 2669994 Unknwon, conserved hypothetical proteins
SeqlD n° 830 LM-2039.1 From 2665666 to 2668653 Unknwon, similar to formate dehydrogenase alpha chain
SeqlD n° 831 LM-204.1 From 1181720 to 1182421 unknown, similar to
Salmonella typhimurium
PduB protein
SeqlD n° 832 LM-2040.1 From 2665190 to 2665666 Unknown, similar to B. subtilis YrhD protein
SeqlD n° 833 LM-2042.1 From 2664343 to 2665128 Unknwon, similar to formate dehydrogenase associated protein
SeqlD n° 834 LM-2043.1 From 2663619 to 2664296 unknown, similar to two- component response regulator
SeqlD n° 835 LM-2044.1 From 2662243 to 2663622 Unknwon, similar to response regulator histidine kinase
SeqlD n° 836 LM-2045.1 From 2661076 to 2662164 Unknown, conserved hypothetical protein
SeqlD n° 837 LM-2046.1 From 2660408 to 2661076 Unknwon, similar to ABC transporter, ATP-binding protein
SeqlD n° 838 LM-2047.1 From 2659852 to 2660145 Unknwon, conserved hypothetical protein
SeqlD n° 839 LM-2048.2 From 2658966 to 2659841 Unknwon
SeqlD n° 840 LM-2049.3 From 403743 to 404198 Unknown
SeqlD n° 841 LM-205.1 From 1181338 to 1181625 unknown, similar to
Salmonella typhimurium
PduA protein
SeqlD n° 842 LM-2050.1 From 403279 to 403725 Unknown SeqlD n° 843 LM-2051.1 From 402641 to 403060 Unknown SeqlD n° 844 LM-2053.1 From 401619 to 402539 Unknown, similar to putative transcription regulator
SeqlD n° 845 LM-2054.1 From 400997 to 401299 Unknown, similar to PTS betaglucoside-specific enzyme IIB component 1/77335
86
SeqlD n° 846 LM-2055.1 From 399645 to 400979 Unknown, similar to PTS betaglucoside-specific enzyme IIC component
SeqlD n° 847 LM-2056.1 From 398210 to 399652 Unknown, similar to beta- glucosidase
SeqlD n° 848 LM-2058.1 From 397340 to 398053 Unknown, similar to transcription regulator
(GntR family)
SeqlD n° 849 LM-2059.1 From 396964 to 397299 Unknown, conserved hypothetical protein
SeqlD n° 850 LM-2060.1 From 396208 to 396927 Unknown, conserved hypothetical protein, highiy similar to B. subtilis Yeel protein
SeqlD n° 851 LM-2061.1 From 395602 to 396111 Unknown, similar to différent proteins
SeqlD n° 852 LM-2063.1 From 394264 to 395529 Unknown, conserved hypothetical protein similar to B. subtilis YwbN protein
SeqlD n° 853 LM-2064.1 From 393086 to 394246 Unknown, conserved hypothetical protein, putative lippoprotein
SeqlD n° 854 LM-2065.1 From 391604 to 393052 Unknown, similar to conserved hypothetical protein
SeqlD n° 855 LM-2066.1 From 390529 to 391473 Unknown, similar to transcription regulator
SeqlD n° 856 LM-2067.1 From 389813 to 390430 Unknown, similar to
Salmonella typhimurium peptidase E
SeqlD n° 857 LM-2068.1 From 388807 to 389541 Unknown, similar to conserved hypothetical intégral membrane protein
SeqlD n° 858 LM-2069.1 From 387697 to 388467 Unknown, similar to transcriptional regulator
(DeoR family)
SeqlD n° 859 LM-2070.1 From 386780 to 387640 Unknown, similar to D- fructose-1 ,6-biphosphate aldolase
SeqlD n° 860 LM-2072.1 From 385386 to 386780 Unknown, similar to PTS system, fructose-specific enzyme IIBC component
SeqlD n° 861 LM-2073.1 From 384926 to 385372 Unknown, similar to PTS system, enzyme IIA component
SeqlD n° 862 LM-2074.1 From 383710 to 384726 Unknown, similar to oxidoreductase
SeqlD n° 863 LM-2076.1 From 382016 to 383536 Unknown, similar to
Flavocytochrome C
Fumarate Reductase chain
A 87
SeqlD n° 864 LM-2077.1 From 380253 to 381779 Unknown, similar to fatty- acid-CoA ligase
SeqlD n° 865 LM-2078.1 From 379794 to 380207 Unknown, similar to unknown proteins
SeqlD n° 866 LM-2080.2 From 378964 to 379728 unknown, highiy similar to regulatory proteins (DeoR family)
SeqlD n° 867 LM-2082.3 From 2052233 to 2052664 Unknown, similar to unknown proteins
SeqlD n° 868 LM-2083.1 From 2052816 to 2053301 Unknown, similar to unknown proteins
SeqlD n° 869 LM-2084.1 From 2053274 to 2053801 Unknown, similar to unknown proteins
SeqlD n° 870 LM-2088.1 From 2054493 to 2056187 Unknown, similar to dihydroxy-acid dehydratase
SeqlD n° 871 LM-2089.1 From 2056205 to 2057926 Unknown, similar to acetolactate synthase
(acetohydroxy-acid synthase) (large subunit)
SeqlD n° 872 LM-2091.1 From 2057927 to 2058418 Unknown, similar to acetolactate synthase
(acetohydroxy-acid synthase) (small subunit)
SeqlD n° 873 LM-2092.1 From 2058516 to 2059511 Unknown, similar to ketol- acid reductoisomerase
(acetohydroxy-acid isomeroreductase)
SeqlD n° 874 LM-2097.1 From 2059669 to 2061207 Unknown, similar to 2- isopropylmalate synthase
SeqlD n° 875 LM-2098.1 From 2061209 to 2062261 Unknown, similar to 3- isopropylmalate dehydrogenase
SeqlD n° 876 LM-21.1 From 2723786 to 2725852 Unknown, similar to transcriptional antiterminator
SeqlD n° 877 LM-2100.1 From 2062263 to 2063651 Unknown, similar to 3- isopropylmalate dehydratase (large subunit)
SeqlD n° 878 LM-2101.1 From 2063638 to 2064219 Unknown, similar to 3- isopropylmalate dehydratase (small subunit)
SeqlD n° 879 LM-2102.1 From 2064238 to 2065506 Unknown, similar to thréonine dehydratase
SeqlD n° 880 LM-2104.1 From 2065698 to 2066417 Unknown, similar to alpha- acetolactate decarboxylase
SeqlD n° 881 LM-2106.1 From 2066455 to 2067756 Unknown, similar to pyrimidine-nucleoside phosphorylase
SeqlD n° 882 LM-2107.3 From 2067887 to 2068897 Unknown, similar to transcription regulators
(Lacl family) 88
SeqlD n° 883 LM-2109.2 From 1677409 to 1680009 Unknown, similar to Alcohol-acetaldehyde dehydrogenase
SeqlD n° 884 LM-2110.1 From 1680132 to 1680560 Unknown, similar to unknown proteins SeqlD n° 885 LM-2111.1 From 1680644 to 1681564 Unknown, similar to similar to ABC transporter (ATP- binding protein)
SeqlD n° 886 LM-2113.1 From 1681561 to 1682628 Unknown, similar to membrane proteins
SeqlD n° 887 LM-2114.1 From 1682664 to 1683638 Unknown, similar to unknown proteins
SeqlD n° 888 LM-2115.1 From 1683635 to 1684216 Unknown, similar to dna-3- methyladenine glycosidase
SeqlD n° 889 LM-2116.1 From 1684229 to 1684462 Unknown SeqlD n° 890 LM-2119.1 From 1684563 to 1687265 unknown, highiy similar to aconitate hydratases
SeqlD n° 891 LM-2120.1 From 1687420 to 1688223 Unknown, similar to putative sigma factor regulator
SeqlD n° 892 LM-2121.1 From 1688248 to 1688670 Unknown SeqlD n° 893 LM-2123.1 From 1688670 to 1691888 Unknown, similar to SNF2- type helicase
SeqlD n° 894 LM-2127.1 From 1691972 to 1695043 Unknown, similar to ATP- dependent dsDNA exonuclease SbcC
SeqlD n° 895 LM-2128.1 From 1695040 to 1696164 unknown, similar to putative exonucleases
SbcD
SeqlD n° 896 LM-2129.1 From 1696280 to 1696888 unknown, similar to 1- acylglycerol-3-phosphate
O-acyltransferases
SeqlD n° 897 LM-2130.1 From 1696940 to 1697302 unknown SeqlD n° 898 LM-2131.1 From 1697476 to 1697991 unknown SeqlD n° 899 LM-2133.1 From 1698165 to 1698653 unknown, similar to hypothetical proteins
SeqlD n° 900 LM-2134.1 From 1698734 to 1700539 unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 901 LM-2135.2 From 1700523 to 1702292 unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 902 LM-2138.1 From 366508 to 367032 unknown SeqlD n° 903 LM-214.1 From 1177116 to 1177865 unknown SeqlD n° 904 LM-2140.1 From 367533 to 367901 Unknown SeqlD n° 905 LM-2141.1 From 367898 to 369373 Unknown SeqlD n° 906 LM-2142.1 From 369377 to 369757 Unknown SeqlD n° 907 LM-2143.1 From 370032 to 370403 Unknown, weakiy similar to inorganic pyrophosphatase
SeqlD n° 908 LM-2145.1 From 370732 to 371064 Unknown SeqlD n° 909 LM-2147.1 From 371130 to 373127 Unknown, similar to transketolase 1 7
89
SeqlD n° 910 LM-2148.1 From 373129 to 373785 Unknown, similar to transaldolase
SeqlD n° 911 LM-215.1 From 1176660 to 1177091 unknown, similar to
Salmonella enterica PduV protein
SeqlD n° 912 LM-2150.1 From 373832 to 374596 Unknown, similar to dehydrogenase/reductase
SeqlD n° 913 LM-2151.1 From 374621 to 375067 Unknown, similar to sugar- phosphate isomerase
SeqlD n° 914 LM-2152.1 From 375074 to 375838 Unknown, similar to triosephosphate isomerase
SeqlD n° 915 LM-2154.1 From 375842 to 376492 Unknown, similar to dihydroxyacetone kinase
SeqlD n° 916 LM-2155.1 From 376514 to 377509 Unknown, similar to dihydroxyacetone kinase
SeqlD n° 917 LM-2156.1 From 377531 to 377872 Unknown
SeqlD n° 918 LM-2157.2 From 377888 to 378319 Unknown
SeqlD n° 919 LM-2158.2 From 378404 to 378781 Unknown, similar to unknown proteins
SeqlD n° 920 LM-216.1 From 1176304 to 1176654 Unknown, similar to
Salmonelle enterica PduU protein
SeqlD n° 921 LM-2161.2 From 72408 to 74222 Unknwon, similar to toxin components
SeqlD n° 922 LM-2162.1 From 72062 to 72394 Unknwon
SeqlD n° 923 LM-2163.1 From 71360 to 72061 Unknwon
SeqlD n° 924 LM-2164.1 From 71064 to 71363 Unknwon
SeqlD n° 925 LM-2166.1 From 70676 to 71071 unknwon
SeqlD n° 926 LM-217.1 From 1175602 to 1176156 Unknown, similar to
Salmonella enterica PduT protein
SeqlD n° 927 LM-2170.1 From 66160 to 70656 Unknwon, highiy similar to
B. subtilis YukA protein
SeqlD n° 928 LM-2171.1 From 64950 to 66146 Unknwon, similar to B. subtilis YukC protein
SeqlD n° 929 LM-2172.1 From 64677 to 64928 Unknwon, similar to B. subtilis YukD protein
SeqlD n° 930 LM-2174.1 From 64144 to 64659 Unknwon
SeqlD n° 931 LM-2178.1 From 60948 to 64154 Unknwon, similar to B. subtilis YueB protein
SeqlD n° 932 LM-2179.1 From 60506 to 60799 Unknwon, similar to a small heat shock protein of Clostridium acetobutylicum
SeqlD n° 933 LM-218.1 From 1174241 to 1175605 unknown, similar to Salmonella enterica PduS protein
SeqlD n° 934 LM-2180.2 From 58897 to 60189 unknown, highiy similar to adenylosuccinate synthetase
SeqlD n° 935 LM-2182.2 From 1814403 to 1815080 Unknown, similar to two- component response regulator SeqlD n° 936 LM-2184.1 From 1813457 to 1814332 Unknown, similar to unknown proteins
SeqlD n° 937 LM-2185.1 From 1812978 to 1813436 Unknown
SeqlD n° 938 LM-2186.1 From 1811219 to 1812961 unknown, highiy similar to adenine deaminases
SeqlD n° 939 LM-2188.1 From 1810157 to 1811197 Unknown, similar to two- component sensor histidine kinase
SeqlD n° 940 LM-2189.1 From 1809118 to 1809759 Unknown, similar to amino acid (glutamine) ABC transporter, permease protein
SeqlD n° 941 LM-2191.1 From 1808460 to 1809107 Unknown, similar to amino acid (glutamine) ABC transporter (ATP-binding protein)
SeqlD n° 942 LM-2193.1 From 1807643 to 1808458 Unknown, similar to amino acid ABC transporter
(binding protein)
SeqlD n° 943 LM-2194.1 From 1806446 to 1807552 Unknown, similar to glycerol dehydrogenase
SeqlD n° 944 LM-2195.1 From 1805925 to 1806443 Unknown, similar to unknown proteins
SeqlD n° 945 LM-2196.1 From 1805041 to 1805928 transcription activator of glutamate synthase operon
GltC
SeqlD n° 946 LM-2199.1 From 1800256 to 1804848 Unknown, similar to glutamate synthase (large subunit)
SeqlD n° 947 LM-22.1 From 2726008 to 2727195 unknown, highiy similar to translation elongation factor EF-Tu
SeqlD n° 948 LM-220.1 From 1173643 to 1174122 unknown, similar to uroporphyrin-lll C- methyltransferase
SeqlD n° 949 LM-2201.1 From 1798772 to 1800241 Unknown, similar to glutamate synthase (small subunit)
SeqlD n° 950 LM-2203.1 From 1797897 to 1798727 Unknown, similar to sugar transport protein
SeqlD n° 951 LM-2204.1 From 1796987 to 1797880 Unknown, similar to sugar transport protein
SeqlD n° 952 LM-2205.2 From 1795681 to 1796925 Unknown, similar to sugar binding protein
SeqlD n° 953 LM-2207.2 From 2505425 to 2505835 Unknown
SeqlD n° 954 LM-2209.1 From 2505858 to 2506370 unknown
SeqlD n° 955 LM-221.1 From 1173144 to 1173551 unknown
SeqlD n° 956 LM-2210.1 From 2506494 to 2506835 Unknown
SeqlD n° 957 LM-2212.1 From 2506867 to 2507250 unknown
SeqlD n° 958 LM-2213.1 From 2507306 to 2508202 Unknown, similar to transcription regulator
SeqlD n° 959 LM-2214.1 From 2508247 to 2508816 Unknown SeqlD n° 960 LM-2215.1 From 2508992 to 2509411 unknown SeqlD n° 961 LM-2216.1 From 2510361 to 2514293 Unknown, similar to glycosidase
SeqlD n° 962 LM-2217.1 From 2514308 to 2515210 Unknown, similar to internalin
SeqlD n° 963 LM-2219.1 From 2515312 to 2518587 Unknown, similar to glycosidase
SeqlD n° 964 LM-2220.1 From 2518971 to 2519879 Unknown, similar to transcription regulator
SeqlD n° 965 LM-2221.1 From 2519935 to 2520399 Unknown, conserved hypothetical protein
SeqlD n° 966 LM-2222.1 From 2520415 to 2522796 Unknwon, similar to exoribonuclease RNase-R
SeqlD n° 967 LM-2224.2 From 2522830 to 2523576 Unknown, similar to carboxylesterase
SeqlD n° 968 LM-2225.2 From 2343056 to 2343685 Unknown, similar to phosphoglucomutase
SeqlD n° 969 LM-2226.1 From 2341865 to 2343013 Unknown, similar to aspartate aminotransferase
SeqlD n° 970 LM-2227.1 From 2341064 to 2341789 Unknown, similar to amino acid ABC transporter (ATP- binding protein)
SeqlD n° 971 LM-2228.1 From 2339611 to 2341071 Unknown, similar to amino acid ABC transporter, permease protein
SeqlD n° 972 LM-2229.1 From 2338420 to 2339418 Unknown, similar to low- affinity inorganic phosphate transporter
SeqlD n° 973 LM-223.1 From 1172797 to 1173036 unknown SeqlD n° 974 LM-2230.1 From 2337786 to 2338406 Unknown, similar to unknown proteins
SeqlD n° 975 LM-2232.1 From 2336515 to 2337399 Unknown, similar to oxidoreductase
SeqlD n° 976 LM-2233.1 From 2335862 to 2336518 Unknown, similar to unknown proteins
SeqlD n° 977 LM-2234.1 From 2335468 to 2335860 Unknown, similar to unknown proteins
SeqlD n° 978 LM-2235.1 From 2334586 to 2335455 Unknown, similar to putative ribosomal large subunit pseudouridine synthase
SeqlD n° 979 LM-2236.1 From 2334003 to 2334569 Unknown, similar to methylphosphotriester-
DNA alkyltransferase and transcriptional regulator
SeqlD n° 980 LM-2237.1 From 2333375 to 2333857 Unknown, similar to O6- methylguanine-DNA methyltransferase
SeqlD n° 981 LM-2238.1 From 2332888 to 2333283 Unknown, similar to transcriptional regulators
(GntR family) SeqlD n° 982 LM-2239.1 From 2332020 to 2332898 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 983 LM-224.1 From 1172076 to 1172648 Unknown, similar to ATP- dependent Clp protease proteolytic component
SeqlD n° 984 LM-2241.1 From 2330959 to 2332023 Unknown SeqlD n° 985 LM-2242.1 From 2329731 to 2330921 Unknown, similar to transport system permease protein
SeqlD n° 986 LM-2244.1 From 2328363 to 2329571 Unknown, similar to transport system permease protein
SeqlD n° 987 LM-2246.1 From 2327461 to 2328330 Unknown, similar to oxidoreductase
SeqlD n° 988 LM-2248.1 From 2325477 to 2327402 Unknown, similar to NADH oxidase
SeqlD n° 989 LM-225.1 From 1171662 to 1172009 unknwon
SeqlD n° 990 LM-2250.1 From 2324554 to 2325408 Unknown, similar to unknown proteins
SeqlD n° 991 LM-2251.1 From 2323375 to 2324253 Unknown, similar to transcriptional regulators
(LysR family)
SeqlD n° 992 LM-2253.1 From 2322031 to 2323335 Unknown, similar to unknown proteins
SeqlD n° 993 LM-2254.2 From 2320906 to 2321775 Unknown, similar to unknown proteins
SeqlD n° 994 LM-2255.2 From 2320416 to 2320841 Unknown, similar to arsenate reductase
SeqlD n° 995 LM-2256.3 From 1405578 to 1405859 Unknown SeqlD n° 996 LM-2257.3 From 1404714 to 1405541 Unknown, similar to B. subtilis SpolllJ protein
SeqlD n° 997 LM-2258.3 From 1403227 to 1404678 two-component sensor histidine kinase
SeqlD n° 998 LM-2260.1 From 1402550 to 1403230 two-component response regulator
SeqlD n° 999 LM-2261.2 From 1400941 to 1402359 Unknown, similar to 6- phosphogluconate dehydrogenase
SeqlD n° 1000 LM-2262.2 From 1399699 to 1400796 Unknown, similar to aminotripeptidase SeqlD n° 1001 LM-2263.1 From 1398077 to 1399327 Unknown, similar to branched-chain alpha-keto acid dehydrogenase E2 subunit (lipoamide acyltransferase)
SeqlD n° 1002 LM-2266.1 From 1397063 to 1398046 Unknown, similar to branched-chain alpha-keto acid dehydrogenase E1 subunit (2-oxoisovalerate dehydrogenase beta subunit) SeqlD n° 1003 LM-2267.1 From 1396051 to 1397046 Unknown, similar to branched-chain alpha-keto acid dehydrogenase E1 subunit (2-oxoisovalerate dehydrogenase alpha subunit)
SeqlD n° 1004 LM-2269.1 From 1394599 to 1396026 Unknown, similar to branched-chain alpha-keto acid dehydrogenase E3 subunit
SeqlD n° 1005 LM-2270.1 From 1393517 to 1394584 Unknown, similar to branched-chain fatty-acid kinase
SeqlD n° 1006 LM-2271.1 From 1392514 to 1393380 Unknown, similar to phosphotransbutyrylase
SeqlD n° 1007 LM-2273.1 From 1390694 to 1392385 DNA repair and genetic recombination
SeqlD n° 1008 LM-2274.1 From 1390222 to 1390671 Unknown, similar to arginine repressor
SeqlD n° 1009 LM-2275.1 From 1389207 to 1390031 Unknown, conserved hypothetical protein
SeqlD n° 1010 LM-2276.1 From 1387396 to 1389210 Unknown, similar to D-1- deoxyxylulose 5-phosphate synthase
SeqlD n° 1011 LM-2278.1 From 1385934 to 1386815 Unknown, similar to geranyltranstransferase
SeqlD n° 1012 LM-2280.1 From 1384362 to 1385714 Unknown, similar to exodeoxyribonuclease VII
(large subunit)
SeqlD n° 1013 LM-2283.1 From 1383489 to 1384343 unknown, highiy similar to methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase
SeqlD n° 1014 LM-2284.1 From 1383007 to 1383393 Unknown, similar to transcription termination protein (NusB)
SeqlD n° 1015 LM-2285.1 From 1382569 to 1382976 unknown, similar to B. subtilis YqhY protein
SeqlD n° 1016 LM-2287.3 From 1381201 to 1382565 acetyl-CoA carboxylase subunit (biotin carboxylase subunit)
SeqlD n° 1017 LM-2289.3 From 1380720 to 1381187 Unknown, similar to acetyl-
CoA carboxylase subunit
(biotin carboxyl carrier subunit)
SeqlD n° 1018 LM-2290.3 From 1380004 to 1380561 unknown, highiy similar to elongation factor P (EF-P)
SeqlD n° 1019 LM-2291.2 From 1275845 to 1276693 unknown, similar to B. subtilis YxkD protein SeqlD n° 020 LM-2292.1 From 1274883 to 1275542 unknown, similar to regulator of the Fnr CRP family (including PrfA)
SeqlD n° 021 LM-2293.1 From 1273473 to 1274678 unknown; similar to antibiotic résistance protein
SeqlD n° 022 LM-2294.1 From 1273231 to 1273476 unknown SeqlD n° 023 LM-2295.1 From 1272717 to 1273193 unknown, weakiy similar to
8-oxo-dGTPase (mutT)
SeqlD n° 024 LM-2296.1 From 1272286 to 1272549 unknown SeqlD n° 025 LM-2298.1 From 1270849 to 1272261 Unknown, similar to ATP- dependent RNA helicase
(DEAD motif)
SeqlD n° 026 LM-2299.1 From 1269834 to 1270433 unknown weakiy similar to phosphoglycerate mutase
1
SeqlD n° 027 LM-230.1 From 1168390 to 1169541 unknown SeqlD n° 028 LM-2300.1 From 1269262 to 1269669 unknown SeqlD n° 029 LM-2301.1 From 1268515 to 1269108 unknown, similar to B. subtilis Ydel protein
SeqlD n° 030 LM-2302.1 From 1267115 to 1268473 unknown SeqlD n° 031 LM-2303.1 From 1266040 to 1266564 unknown, conserved hypothetical protein, similar to B. subtilis YsnB protein
SeqlD n° 032 LM-2304.1 From 1265392 to 1266003 unknown, conserved hypothetical protein, similar to B. subtilis YsnA protein
SeqlD n° 033 LM-2305.1 From 1264642 to 1265388 Unknown, similar to ribonuclease PH SeqlD n° 034 LM-2306.1 From 1263829 to 1264629 Unknown, similar to glutamate racemase SeqlD n° 035 LM-2307.1 From 1263187 to 1263681 unknown, similar to B. subtilis YslB protein SeqlD n° 036 LM-2308.1 From 1261917 to 1263131 Unknown, similar to aspartokinase II alpha subunit
SeqlD n° 037 LM-231.1 From 1168021 to 1168368 unknown, similar to regulatory proteins SeqlD n° 038 LM-2310.1 From 1259917 to 1261728 unknown, highiy similar to excinuclease ABC subunit
C
SeqlD n° 039 LM-2312.1 From 1259530 to 1259841 thioredoxin SeqlD n° 040 LM-2315.1 From 1257092 to 1259449 unknown, similar to MutS protein (MutS2)
SeqlD n° 041 LM-2317.1 From 1255357 to 1257069 unknown, similar to DNA polymérase beta, to B. subtilis YshC protein
SeqlD n° 042 LM-2318.3 From 1254722 to 1255264 unknown, similar to B. subtilis YshB protein 01
95
SeqlD n° 1043 LM-2319.2 From 347658 to 348428 Unknown, similar to unknown proteins
SeqlD n° 1044 LM-232.1 From 1167645 to 1167953 unknown, similar to B. subtilis YjcS protein
SeqlD n° 1045 LM-2320.1 From 347219 to 347611 Unknown, similar to unknown proteins
SeqlD n° 1046 LM-2322.1 From 346377 to 347036 Unknown, similar to unknown proteins
SeqlD n° 1047 LM-2324.1 From 343221 to 344636 Unknown, similar to phospho-beta-glucosidase
SeqlD n° 1048 LM-2325.1 From 342551 to 343195 Unknown, similar to thiamin-phosphate pyrophosphorylase (ThiE)
SeqlD n° 1049 LM-2327.1 From 341751 to 342554 Unknown, similar to phosphomethylpyrimidine kinase (ThiD)
SeqlD n° 1050 LM-2329.1 From 340945 to 341754 Unknown, similar to hydroxyethylthiazole kinase
(ThiM)
SeqlD n° 1051 LM-2332.1 From 340278 to 340952 Unknown, similar to thiamin biosynthesis protein
SeqlD n° 1052 LM-2333.1 From 339270 to 340055 Unknown, similar to unknown protein
SeqlD n° 1053 LM-2334.1 From 338341 to 339087 Unknown, conserved hypothetical protein
SeqlD n° 1054 LM-2335.2 From 337161 to 338363 Unknown, similar to unknown proteins
SeqlD n° 1055 LM-2336.3 From 336541 to 337161 Unknown
SeqlD n° 1056 LM-2338.2 From 1026871 to 1029045 ATP-dependent protease
SeqlD n° 1057 LM-2339.1 From 1026376 to 1026855 unknown, similar to methylated-DNA-protein- cystein methyltransferase
SeqlD n° 1058 LM-234.1 From 1165962 to 1167608 unknown, similar to ABC transporters, ATP-binding proteins (HI0664)
SeqlD n° 1059 LM-2340.1 From 1025159 to 1026190 unknown, similar to B. subtilis YkrP protein
SeqlD n° 1060 LM-2341.1 From 1024761 to 1025129 unknown SeqlD n° 1061 LM-2342.1 From 1023346 to 1024680 unknown, similar to Na+- transporting ATP synthase subunit J
SeqlD n° 1062 LM-2344.1 From 1022535 to 1023302 unknown, conserved hypothetical protein
SeqlD n° 1063 LM-2345.1 From 1021665 to 1022432 unknown, conserved hypothetical protein
SeqlD n° 1064 LM-2346.1 From 1020022 to 1021383 unknown, conserved hypothetical protein
SeqlD n° 1065 LM-2347.1 From 1019545 to 1019970 unknown, similar to regulatory proteins (MarR family) 96
SeqlD n° 066 LM-2348.1 From 1017870 to 1019438 unknown, similar to peptide chain release factor 3 (RF-
3)
SeqlD n° 067 LM-2349.1 From 1016934 to 1017788 unknown, similar to
Streptococcus agalactiae
CylB protein
SeqlD n° 068 LM-235.2 From 1164245 to 1165960 ATP-binding transport protein SeqlD n° 069 LM-2350.1 From 1016030 to 1016941 unknown, similar to antibiotic ABC transporter,
ATP-binding protein,
SeqlD n° 070 LM-2351.1 From 1015605 to 1016033 unknown SeqlD n° 071 LM-2352.1 From 1015153 to 1015608 unknown, weakiy similar to two-component response regulator
SeqlD n° 072 LM-2353.1 From 1014540 to 1015019 unknown, similar to glutathione peroxidase SeqlD n° 073 LM-2355.1 From 1013475 to 1014527 unknown, similar to glucanase and peptidase SeqlD n° 074 LM-2358.1 From 1011860 to 1013335 unknown, similar to efflux transporter SeqlD n° 075 LM-2359.1 From 1011074 to 1011826 unknown, similar to ABC transporter transmembrane component
SeqlD n° 076 LM-236.2 From 1163277 to 1163996 unknown, similar to transcription regulators SeqlD n° 077 LM-2361.1 From 1010313 to 1011077 unknown, similar to daunorubicin résistance
ATP-binding proteins
SeqlD n° 078 LM-2362.1 From 1009098 to 1010117 unknown; similar to branched-chain amino acid aminotransferase
SeqlD n° 079 LM-2363.1 From 1008130 to 1008879 unknown, similar to B. subtilis YjcH protein SeqlD n° 080 LM-2364.1 From 1007669 to 1008112 unknown, similar to B. subtilis YjcF protein SeqlD n° 081 LM-2365.2 From 1006959 to 1007633 unknown, similar to ribose
5-phosphate isomerase SeqlD n° 082 LM-2366.2 From 1937783 to 1938415 unknown, similar to hemolysinlll proteins, putative intégral membrane protein
SeqlD n° 083 LM-2368.1 From 1938538 to 1939155 unknown, similar to conserved hypothetical proteins
SeqlD n° 084 LM-2369. From 939168 to 1939980 unknown, similar to conserved hypothetical proteins
SeqlD n° 085 LM-237.1 From 1162666 to i 163280 Unknown, similar to unknown proteins SeqlD n° 086 LM-2371.1 From 1939999 to 1942638 unknown, similar to pyruvate phosphate dikinase
SeqlD n° 1087 LM-2372.1 From 1942687 to 1943064 unknown, similar to conserved hypothetical proteins
SeqlD n° 1088 LM-2373.1 From 1943068 to 1944051 unknown, similar to conserved hypothetical proteins, putative intégral membrane protein
SeqlD n° 1089 LM-2375.1 From 1944145 to 1944768 unknown; similar to alkaline phosphatase
SeqlD n° 1090 LM-2378.1 From 1944908 to 1946419 unknown, similar to phosphoglucomutases
SeqlD n° 1091 LM-2379.1 From 1946488 to 1947363 unknown, similar to methyltransferases
SeqlD n° 1092 LM-238.1 From 1161689 to 1162696 unknown SeqlD n° 1093 LM-2380.1 From 1947416 to 1947898 unknown, similar to dihydrofolate reductases
SeqlD n° 1094 LM-2381.1 From 1947914 to 1948858 unknown, similar to thymidylate synthase
SeqlD n° 1095 LM-2385.1 From 1948871 to 1950763 unknown, similar to putative ABC transporters
(ATP-binding protein)
SeqlD n° 1096 LM-2386.1 From 1950883 to 1951305 Unknown, similar to formyl- tetrahydrofolate synthetase
C-terminal part
SeqlD n° 1097 LM-2387.2 From 1951284 to 1952564 Unknown, similar to formyl- tetrahydrofolate synthetase
N-terminal part
SeqlD n° 1098 LM-2388.2 From 1952721 to 1953149 unknown, similar o transcriptional regulators
SeqlD n° 1099 LM-239.1 From 1161220 to 1161675 unknown SeqlD n° 1100 LM-2390.2 From 148354 to 150009 Unknown, similar to ABC transporter oligopeptide- binding protein
SeqlD n° 1101 LM-2391.1 From 147884 to 148291 Unknwon SeqlD n° 1102 LM-2392.1 From 147399 to 147764 unknwon SeqlD n° 1103 LM-2393.1 From 146764 to 147381 SeqlD n° 1104 LM-2394.1 From 146306 to 146656 unknown SeqlD n° 1105 LM-2395.1 From 145869 to 146306 Unknwon SeqlD n° 1106 LM-2397.1 From 144397 to 144840 Unknown SeqlD n° 1107 LM-2398.1 From 144097 to 144273 unknown SeqlD n° 1108 LM-24.1 From 2727304 to 2729391 unknown, highiy similar to translation elongation factor G
SeqlD n° 1109 LM-240.1 From 1160778 to 1161230 unknown, similar to E. coli
YjaB protein
SeqlD n° 1110 LM-2400.1 From 143366 to 143722 unknown
SeqlD n° 1111 LM-2401.1 From 141618 to 143006 Unknwon
SeqlD n° 1112 LM-2402.1 From 141337 to 141705 Unknwon
SeqlD n° 1113 LM-2404.1 From 141052 to 141336 Unknwon 98
SeqlD n° 1114 LM-2406.1 From 139960 to 140853 Unknwon, similar to oligopeptide ABC transporter, permease protein
SeqlD n° 1115 LM-2409.1 From 138999 to 139949 Unknwon, similar to oligopeptide ABC transporter, permease protein
SeqlD n° 1116 LM-241.2 From 1160220 to 1160753 unknown SeqlD n° 1117 LM-2411.2 From 137323 to 138897 Unknwon, similar to oligopeptide ABC transport system substrate-binding proteins
SeqlD n° 1118 LM-2412.2 From 2164106 to 2165377 Unknown, weakiy similar to transcription regulators
SeqlD n° 1119 LM-2414.1 From 2166012 to 2167343 Unknown, similar to unknown proteins
SeqlD n° 1120 LM-2415.1 From 2167353 to 2167943 Unknown, similar to transcription regulators
SeqlD n° 1121 LM-2416.1 From 2168054 to 2169097 Unknown, similar to lipases SeqlD n° 1122 LM-2417.1 From 2169312 to 2170526 Unknown, similar to argininosuccinate synthase
SeqlD n° 1123 LM-2418.1 From 2170530 to 2171900 Unknown, similar to argininosuccinate lyase
SeqlD n° 1124 LM-242.2 From 1159393 to 1159830 unknown SeqlD n° 1125 LM-2421.1 From 2172068 to 2173591 glycine betaine transporter
BetL
SeqlD n° 1126 LM-2423.1 From 2173836 to 2174486 Unknown, similar to L- fuculose-phosphate aldolase
SeqlD n° 1127 LM-2424.1 From 2174488 to 2175420 Unknown, similar to 1- phosphofructokinase
SeqlD n° 1128 LM-2426.1 From 2175438 to 2176787 Unknown, similar to PTS system galactitol-specific enzyme IIC component
SeqlD n° 1129 LM-2428.1 From 2176815 to 2177090 Unknown, similar to PTS system galactitol-specific enzyme MB component
SeqlD n° 1130 LM-2429.1 From 2177096 to 2177563 Unknown, similar to PTS system galactitol-specific enzyme IIA component
SeqlD n° 1131 LM-243.1 From 1158930 to 1159403 unknown SeqlD n° 1132 LM-2431.1 From 2177571 to 2179577 Unknown, similar to transcription antiterminator
SeqlD n° 1133 LM-2432.2 From 2179758 to 2181203 Unknown, similar to transcriptional regulator
(GntR family) and to aminotransferase (MocR- like)
SeqlD n° 1134 LM-2433.2 From 161420 to 162250 unknwon SeqlD n° 1135 LM-2439.1 From 155993 to 156805 Unknwown, conserved hypothetical protein 99
SeqlD n° 1136 LM-244.1 From 1158425 to 1158844 unknown
SeqlD n° 1137 LM-2441.1 From 153608 to 155947 Unknwon, similar to ATP dépendent helicase
SeqlD n° 1138 LM-2442.1 From 152648 to 153328 Unknwon
SeqlD n° 1139 LM-2444.1 From 151839 to 152645 Unknwon, similar to high- affinity zinc ABC transporter (membrane protein)
SeqlD n° 1140 LM-2446.1 From 151186 to 151890 Unknwon, similar to high- affinity zinc ABC transporter (ATP-binding protein)
SeqlD n° 1141 LM-2447.2 From 150232 to 151173 Unknown, similar to a probable high-affinity zinc
ABC transporter (Zn(ll)- binding lipoprotein)
SeqlD n° 1142 LM-2448.2 From 2635167 to 2637920 autolysin, amidase SeqlD n° 1143 LM-245.1 From 1158020 to 1158388 unknown SeqlD n° 1144 LM-2450.1 From 2637964 to 2639562 unknown, highiy similar to
CTP synthases
SeqlD n° 1145 LM-2452.1 From 2639930 to 2640466 Unknown, similar to B. subtilis RNA polymérase delta subunit
SeqlD n° 1146 LM-2453.1 From 2640832 to 2642502 arginyl tRNA synthetase SeqlD n° 1147 LM-2455.1 From 2642499 to 2642948 Unknwon SeqlD nc 1148 LM-2456.1 From 2643026 to 2643679 Unknwon, conserved hypothetical protein
SeqlD n° 1149 LM-2457.1 From 2643973 to 2645295 Unknown, conserved hypothetical protein
SeqlD n° 1150 LM-2458.1 From 2645310 to 2646146 Unknwon
SeqlD n° 1151 LM-2459.1 From 2646686 to 2647009 Unknown
SeqlD n° 1152 LM-246.1 From 1157592 to 1158008 unknown
SeqlD n° 1153 LM-2460.1 From 2647164 to 2648825 Unknwon, similar to dipeptide ABC transporter
(dipeptide-binding protein)
SeqlD n° 1154 LM-2461.1 From 2648960 to 2649574 Unknwon SeqlD n° 1155 LM-2462.1 From 2649598 to 2650230 Unknwon, similar to nicotinamidase
SeqlD n° 1156 LM-2463.1 From 2650231 to 2650755 Unknwon, similar to Chain
A, Dihydrofolate Reductase
SeqlD n° 1157 LM-2464.1 From 2650758 to 2651756 Unknown, similar to zinc- binding dehydrogenase
SeqlD n° 1158 LM-2465.1 From 2651853 to 2652125 Unknwon SeqlD n° 1159 LM-2467.2 From 2652174 to 2653085 Unknwon, similar to cation transport protein
SeqlD n° 1160 LM-2469.1 From 331543 to 332688 Unknown SeqlD n° 1161 LM-247.3 From 1156384 to 1157241 unknown, similar to methylases
SeqlD n° 1162 LM-2470.1 From 331063 to 331446 Unknown SeqlD n° 1163 LM-2473.1 From 329923 to 330999 Unknown, similar to low specificity L-allo-threonine aldolase 100
SeqlD n° 1164 LM-2474.1 From 328701 to 329966 Unknown
SeqlD n° 1165 LM-2475.1 From 327941 to 328495 Unknown, putaive secreted, lysin rich protein
SeqlD n° 1166 LM-2476.1 From 327621 to 327905 Unknown
SeqlD n° 1167 LM-2477.1 From 327113 to 327454 Unknown, similar to PTS beta-glucoside-specific enzyme IIA component
SeqlD n° 1168 LM-2478.1 From 325729 to 327120 Unknown, similar to phospho-beta-glucosidase and phospho-beta- galactosidase
SeqlD n° 1169 LM-2480.1 From 325422 to 325712 Unknown, similar to PTS beta-glucoside-specific enzyme IIB component
SeqlD n° 1170 LM-2482.1 From 324104 to 325417 Unknown, similar to PTS beta-glucoside-specific enzyme IIC component
SeqlD n° 1171 LM-2483.1 From 322134 to 324005 Unknown, similar to transcriptional antiterminator (BglG family)
SeqlD n° 1172 LM-2485.1 From 321490 to 322128 Unknown SeqlD n° 1173 LM-2486.1 From 320542 to 321279 Unknown, similar to FMN- containing NADPH-linked nitro/flavin reductase
SeqlD n° 1174 LM-2487.1 From 319560 to 320423 Unknown, similar to transcription regulator
LysR-gltR family
SeqlD n° 1175 LM-2488.2 From 319049 to 319528 Unknown, conserved hypothetical protein, highiy similar to B. subtilis YydA proteinYyd
SeqlD n° 1176 LM-2489.2 From 2118812 to 2119786 Unknown, similar to phospho-N- acetylmuramoyl- pentapeptide transferase
SeqlD n° 1177 LM-249.2 From 2788504 to 2790243 unknown, highiy similar to ABC transporter (ATP- binding protein) required for expression of cytochrome BD
SeqlD n° 1178 LM-2490.1 From 2117304 to 2118671 Unknown, similar to UDP- N-acetylmuramoylalanine D-glutamate ligase
SeqlD n° 1179 LM-2491.1 From 2116216 to 2117307 Unknown, similar to peptidoglycan synthesis enzymes, putative phospho-N- acetylmuramoyl- pentapeptide-transferase
SeqlD n° 1180 LM-2493.1 From 2115381 to 2116193 Unknown, similar to cell- division initiation protein divIB SeqlD n° 1181 LM-2495.1 From 2113734 to 2115014 unknown, highiy similar to œil-division protein FtsA
SeqlD n° 1182 LM-2496.1 From 2112492 to 2113667 unknown, highiy similar to cell-division initiation protein FtsZ
SeqlD n° 1183 LM-2497.1 From 2111684 to 2112373 Unknown, similar to unknown proteins
SeqlD n° 1184 LM-2499.1 From 2111222 to 2111680 Unknown, similar to unknown proteins
SeqlD n° 1185 LM-25.1 From 2729457 to 2729927 ribosomal protein S7
SeqlD n° 1186 LM-2500.1 From 2110909 to 2111199 Unknown, similar to unknown proteins
SeqlD n° 1187 LM-2501.1 From 2110009 to 2110785 Unknown, similar to unknown proteins
SeqlD n° 1188 LM-2506.1 From 2104901 to 2106001 Unknown, similar to quinolinate synthetase
SeqlD n° 1189 LM-2507.1 From 2104059 to 2104904 Unknown, similar to nicotinate-nucleotide pyrophosphorylase
SeqlD n° 1190 LM-2508.2 From 2102608 to 2104062 Unknown, similar to L- aspartate oxidase
SeqlD n° 1191 LM-2509.2 From 2101371 to 2102477 Unknown, similar to a NifS- like protein required for
NAD biosynthesis
SeqlD n° 1192 LM-251.1 From 2790243 to 2791967 unknown, highiy similar to
ABC transporter required for expression of cytochrome BD
SeqlD n° 1193 LM-2510.2 From 2034693 to 2035517 Unknown, similar to ferrichrome ABC transporter (ATP-binding protein)
SeqlD n° 1194 LM-2511.1 From 2035507 to 2036505 Unknown, similar to oxidoreductases
SeqlD n° 1195 LM-2512.1 From 2036521 to 2037141 Unknown, similar to transcription regulators
(TetR family)
SeqlD n° 1196 LM-2514.2 From 2037141 to 2037956 Unknown, similar to unknown proteins
SeqlD n° 1197 LM-2515.2 From 2037953 to 2038840 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 1198 LM-2517.1 From 2039441 to 2039998 Unknown, similar to unknown proteins
SeqlD n° 1199 LM-2518.1 From 2040274 to 2040933 Unknown, similar to unknown proteins
SeqlD n° 1200 LM-252.1 From 2791967 to 2792980 unknown, highiy similar to cytochrome D ubiquinol oxidase subunit II
SeqlD n° 1201 LM-2520.1 From 2040911 to 2042110 Unknown, similar to toxic ion résistance proteins 102
SeqlD n° 1202 LM-2521.1 From 2042157 to 2042900 unknown, similar to creatinine amidohydrolases
SeqlD n° 1203 LM-2522.1 From 2042913 to 2043521 Unknown, similar to 2-keto-
3-deoxygluconate-6- phosphate aldolase
SeqlD n° 1204 LM-2523.1 From 2043540 to 2044457 Unknown, similar to putative phosphotriesterase related proteins
SeqlD n° 1205 LM-2524.1 From 2044481 to 2045749 Unknown, similar to unknown proteins
SeqlD n° 1206 LM-2527.1 From 2046046 to 2046489 Unknown, similar to PTS system enzyme II A component
SeqlD n° 1207 LM-2528.1 From 2046507 to 2047256 Unknown, similar to transcription regulators,
(GntR family)
SeqlD n° 1208 LM-2529.1 From 2047444 to 2048514 Unknown, similar to E. coli
DNA-damage-inducibile protein dinP
SeqlD n° 1209 LM-2530.1 From 2048623 to 2049414 Unknown, similar to oxidoreductase
SeqlD n° 1210 LM-2531.1 From 2049419 to 2050339 Unknown, similar to unknown proteins
SeqlD n° 1211 LM-2532.4 From 2050639 to 2052114 Unknown, similar to glucose-6-phosphate 1- dehydrogenase
SeqlD n° 1212 LM-2533.2 From 1731955 to 1732893 unknown, similar to menaquinone biosynthesis proteins
SeqlD n° 1213 LM-2536.1 From 1732926 to 1734779 unknown, similar to 5- methyltetrahydrofolate- homocysteine methyltransferase (metH)
SeqlD n° 1214 LM-2538.1 From 1734776 to 1735948 unknown; similar to cystathionine beta-lyase
SeqlD n° 1215 LM-2539.1 From 1735941 to 1737065 unknown, similar to cystathionine gamma- synthase
SeqlD n° 1216 LM-254.1 From 2792967 to 2794373 unknown, highiy similar to cytochrome D ubiquinol oxidase subunit I
SeqlD n° 1217 LM-2540.2 From 1737087 to 1739384 unknown, similar to cobalamin-independent methionine synthase
SeqlD n° 1218 LM-2542.2 From 2088797 to 2091454 Unknown, similar to putative sugar hydrolases
SeqlD n° 1219 LM-2543.1 From 2087489 to 2088793 Unknown, similar to unknown proteins
SeqlD n° 1220 LM-2544.1 From 2086817 to 2087428 unknown, similar to unknown proteins SeqlD n° 1221 LM-2545.1 From 2085021 to 2086760 unknown, similar to two- component sensor histidine kinase
SeqlD n° 1222 LM-2546.1 From 2083537 to 2085021 unknown, similar to two- component response regulator
SeqlD n° 1223 LM-2547.1 From 2082495 to 2083424 Unknown, similar to putative transport system intégral membrane protein
SeqlD n° 1224 LM-2548.1 From 2081505 to 2082476 Unknown, similar to ABC transporter, permease protein
SeqlD n° 1225 LM-2549.1 From 2080027 to 2081484 Unknown, similar to putative sugar-binding lipoproteins
SeqlD n° 1226 LM-255.1 From 2794755 to 2795225 Unknown, conserved hypothetical proteins
SeqlD n° 1227 LM-2553.1 From 2078132 to 2079829 Unknown, similar to alpha- acetolactate synthase protein, AIsS
SeqlD n° 1228 LM-2554.3 From 2077019 to 2078014 Unknown, similar to oxidoreductase
SeqlD n° 1229 LM-2557.2 From 634479 to 635279 Unknown, similar to transport proteins
(formate?)
SeqlD n° 1230 LM-2559.2 From 633709 to 634251 Unknown SeqlD n° 1231 LM-2560.1 From 632811 to 633581 Unknown, similar to unknown membrane proteins
SeqlD n° 1232 LM-2561.1 From 631045 to 632811 Unknown, similar to a fusion of two types of conserved hypothetical proteinconserved hypothetical
SeqlD n° 1233 LM-2562.1 From 630629 to 631042 Unknown SeqlD n° 1234 LM-2563.1 From 629088 to 630491 Unknown, similar to DNA photolyase
SeqlD n° 1235 LM-2565.1 From 626506 to 628971 Unknown, putative secreted protein
SeqlD n° 1236 LM-2566.1 From 625463 to 626488 Unknown
SeqlD n° 1237 LM-2567.2 From 624682 to 625395 Unknown, putative secreted protein
SeqlD n° 1238 LM-2568.3 From 702088 to 702954 Unknown, conserved hypothetical proteins
SeqlD n° 1239 LM-2570.1 From 701247 to 702062 unknown, highiy similar to phosphomethylpyrimidine kinase thiD
SeqlD n" 1240 LM-2571.1 From 700876 to 701184 Unknown, similar to unknown proteins SeqlD n° 1241 LM-2573.1 From 700537 to 700821 unknown, similar to transposases SeqlD n° 242 LM-2574.1 From 699410 to 700306 Unknown, similar to transcription regulator (Rgg type)
SeqlD nc 243 LM-2575.1 From 698785 to 699420 Unknown, conserved hypothetical protein
SeqlD n° 244 LM-2577.1 From 698317 to 698733 Unknown SeqlD n° 245 LM-2578.1 From 697634 to 698197 Unknown, conserved hypothetical protein
SeqlD n° 246 LM-2579.1 From 696883 to 697590 Unknown, similar to phosphoprotein phosphatases
SeqlD n° 247 LM-258.1 From 2795420 to 2796997 Unknown, similar to acetate-CoA ligase
SeqlD n° 248 LM-2581.1 From 695496 to 696416 Unknown SeqlD n° 249 LM-2583.1 From 694973 to 695509 Unknown, similar to unknown proteins
SeqlD n° 250 LM-2584.1 From 694317 to 694964 Unknown, similar to transcription regulator
SeqlD n° 251 LM-2587.1 From 691581 to 694271 Unknown, conserved membrane protein
SeqlD n° 252 LM-2589.1 From 690897 to 691538 Unknown, similar to transcription regulators
SeqlD n° 253 LM-259.1 From 2797039 to 2797758 Unknown, similar to glucosamine-6-phosphate isomerase
SeqlD n° 254 LM-2590.1 From 689923 to 690873 Unknown, similar to membrane proteins
SeqlD n° 255 LM-2592.1 From 689541 to 689825 Unknown SeqlD n° 256 LM-2593.1 From 688619 to 689458 Unknown, similar to unknown proteins
SeqlD n° 257 LM-2594.2 From 687138 to 688529 Unknown, similar to amino acid transporter
SeqlD n° 258 LM-2597.3 From 1113759 to 1115630 unknown, similar to B. subtilis minor teichoic acids biosynthesis protein GgaB
SeqlD n° 259 LM-2598.3 From 1115647 to 1116513 Unknown, similar to glucose-1-phosphate thymidyl transferase
SeqlD n° 260 LM-2599.1 From 1116532 to 1117092 Unknown, similar to dTDP- sugar epimerase
SeqlD n° 261 LM-26.1 From 2729958 to 2730371 ribosomal protein S12 SeqlD n° 262 LM-260.1 From 2797838 to 2798572 Unknown, similar to merR- family transcriptional regulator
SeqlD n° 263 LM-2600.1 From 1117093 to 1118079 Unknown, similar to dTDP-
D-glucose 4,6-dehydratase SeqlD n° 264 LM-2601.1 From 1118082 to 1118912 unknown, similar to DTDP-
L-rhamnose synthetase SeqlD n° 265 LM-2602.1 From 1118992 to 1121022 unknown, similar to teichoic acid biosynthesis protein B SeqlD n° 1266 LM-2603.1 From 1121098 to 1121808 unknown, similar to CDP- ribitol pyrophosphorylase
SeqlD n° 1267 LM-2604.1 From 1121805 to 1122830 unknown, similar to glucitol dehydrogenase
SeqlD n° 1268 LM-2606.1 From 1122918 to 1124078 unknown, similar to teichoic acid biosynthesis protein B precursor
SeqlD n° 1269 LM-2607.1 From 1124079 to 1124462 unknown, highiy similar to glycerol-3-phosphate cytidylyltransferase (gct),
CDP-glycerol pyrophosphorylase
(teichoic acid biosynthesis protein D)
SeqlD n° 1270 LM-2608.1 From 1124484 to 1125467 unknown, similar to glycosyltransferases
SeqlD n° 1271 LM-2609.1 From 1125482 to 1126495 unknown, siumilar to glysosyltransferases
SeqlD n° 1272 LM-261.1 From 2798626 to 2799087 Unknown, similar to unknown proteins
SeqlD n° 1273 LM-2611.1 From 1126704 to 1128194 unknown, conserved hypothetical protein, similar to B. subtilis YueK protein
SeqlD n° 1274 LM-2613.1 From 1128206 to 1129030 unknown, similar to NH(3)- dependent NAD(+) synthetases, nitrogen regulatory protein
SeqlD n° 1275 LM-2614.1 From 1129043 to 1129351 unknown SeqlD n° 1276 LM-2615.1 From 1129412 to 1129816 unknown, similar to PTS system, cellobiose-specific
IIB component (cel A)
SeqlD n° 1277 LM-2616.1 From 1129945 to 1131501 unknown, highiy similar to
GMP synthetase
SeqlD n° 1278 LM-2618.1 From 1131558 to 1132760 unknown, similar to integ rases
SeqlD n° 1279 LM-2619.1 From 1133698 to 1134117 unknown, similar to a protein encoded by Tn916
SeqlD n° 1280 LM-262.1 From 2799108 to 2799551 Unknown, similar to unknown proteins
SeqlD n° 1281 LM-2621.4 From 1134472 to 1136595 cadmium résistance protein
SeqlD n° 1282 LM-2623.2 From 2119833 to 2121308 Unknown, similar to UDP-
N-acetylmuramoylalanyl-D- glutamate-2,6- diaminopimelate ligase
SeqlD n° 1283 LM-2625.2 From 2121483 to 2123738 Unknown, similar to penicillin-binding protein 2B
SeqlD n° 1284 LM-2626.2 From 2123735 to 2124097 Unknown, similar to cell- division protein FtsL
SeqlD n° 1285 LM-2628.1 From 2124114 to 2125052 Unknown, similar to unknown proteins SeqlD n° 1286 LM-2629.1 From 2125065 to 2125496 Unknown, similar to unknown proteins
SeqlD n° 1287 LM-263.1 From 2799609 to 2800961 Unknown, conserved hypothetical proteins
SeqlD n° 1288 LM-2631.2 From 2125699 to 2126946 Unknown, similar to intégral membrane proteins
SeqlD n° 1289 LM-2632.1 From 2127062 to 2128876 Unknown, similar to transporter binding proteins
SeqlD n° 1290 LM-2633.1 From 2128806 to 2129192 Unknown
SeqlD n° 1291 LM-2635.1 From 2129185 to 2130078 Unknown, weakiy similar to ketopantoate reductase involved in thiamin biosynthesis
SeqlD n° 1292 LM-2637.1 From 2130492 to 2131028 Unknown, similar to unknown proteins
SeqlD n° 1293 LM-2638.1 From 2131158 to 2132330 Unknown, similar to unknown proteins
SeqlD n° 1294 LM-2639.1 From 2132412 to 2134652 Unknown, similar to excinuclease ABC (subunit
A)
SeqlD n° 1295 LM-264.1 From 2800958 to 2801407 Unknown, similar to transcription regulators
SeqlD n° 1296 LM-2641.1 From 2134695 to 2135735 Unknown, weakiy similar to proteases
SeqlD n° 1297 LM-2642.1 From 2135754 to 2136236 Unknown, similar to phosphopantetheine adenylyltransferase
SeqlD n° 1298 LM-2643.1 From 2136239 to 2136796 Unknown, similar to unknown proteins
SeqlD n° 1299 LM-2645.1 From 2136893 to 2137174 Unknown, similar to unknown proteins
SeqlD n° 1300 LM-2647.1 From 2137205 to 2137654 Unknown, similar to unknown proteins
SeqlD n° 1301 LM-2648.1 From 2137702 to 2138757 Unknown, similar to unknown proteins
SeqlD n° 1302 LM-265.1 From 2801577 to 2802458 Unknown, similar to unknown proteins
SeqlD n° 1303 LM-2650.2 From 2138904 to 2139809 unknown, highiy similar to heme A famesyltransferase
SeqlD n° 1304 LM-2651.2 From 1994751 to 1995434 Unknown, similar to unknown proteins
SeqlD n° 1305 LM-2652.1 From 1995520 to 1996116 Unknown, similar to unknown proteins
SeqlD n° 1306 LM-2653.1 From 1996204 to 1996749 Unknown, similar to unknown proteins
SeqlD n° 1307 LM-2654.1 From 1996784 to 1998037 Unknown, similar to unknown proteins
SeqlD n° 1308 LM-2657.1 From 1998168 to 1999454 Unknwon, similar to 5- enolpyruvylshikimate-3- phosphate synthase
SeqlD n° 1309 LM-2659.1 From 1999468 to 2000571 Unknown, similar to prephenate dehydrogenase SeqlD n° 1310 LM-266.1 From 2802543 to 2802998 Unknown, similar to transcription regulator
MerR family
SeqlD n° 1311 LM-2660.1 From 2000592 to 2001674 Unknown, similar to histidinol-phosphate aminotransferase and tyrosine/phenylalanine aminotransferase
SeqlD n° 1312 LM-2661.1 From 2001674 to 2002048 Unknown, similar to chorismate mutase
SeqlD n° 1313 LM-2662.1 From 2002045 to 2003142 Unknown, similar to 3- dehydroquinate synthase
SeqlD n° 1314 LM-2664.2 From 2003145 to 2004311 Unknown, similar to chorismate synthase
SeqlD n° 1315 LM-267.1 From 2802998 to 2803402 Unknown, similar to unknown proteins
SeqlD n° 1316 LM-2675.4 From 1748263 to 1748709 unknown, similar to transcription regulators
(Fur family), PerR in B. subtilis
SeqlD n° 1317 LM-2676.2 From 1356769 to 1357683 unknown, highiy similar to tRNA pseudouridine 55 synthase
SeqlD n° 1318 LM-2677.1 From 1356327 to 1356671 unknown, highiy similar to ribosome-binding factor A
SeqlD n° 1319 LM-2679.1 From 1356032 to 1356310 unknown, conserved hypothetical protein similar to B. subtilis YlxP protein
SeqlD n° 1320 LM-268.1 From 2803445 to 2804056 unknown, similar to phosphatase
SeqlD n° 1321 LM-2683.1 From 1353696 to 1356035 unknown, highiy similar to translation initiation factor
IF-2
SeqlD n° 1322 LM-2684.1 From 1353374 to 1353673 unknown, conserved hypothetical protein, similar to B. subtilis YlxQ protein
SeqlD n° 1323 LM-2685.1 From 1353097 to 1353381 unknown, similar to B. subtilis YlxR protein
SeqlD n° 1324 LM-2687.1 From 1351964 to 1353082 unknown, highiy similar to
N utilization substance protein A (NusA protein)
SeqlD n° 1325 LM-2689.1 From 1351467 to 1351934 unknown, conserved hypothetical protein, similar to B. subtilis YlxS protein
SeqlD n° 1326 LM-269.1 From 2804070 to 2804438 unknown, similar to transcription regulator
(RpîR family)
SeqlD n° 1327 LM-2692.1 From 1346951 to 1351285 unknown, highiy similar to
DNA polymérase III (alpha subunit) SeqlD n° 1328 LM-2693.1 From 1345140 to 1346846 prolyl-tRNA synthetase SeqlD n° 1329 LM-2696.2 From 1343838 to 1345100 unknown, conserved hypothetical protein similar to B. subtilis YluC protein
SeqlD n° 1330 LM-2698.2 From 1342682 to 1343824 Unknown, similar to deoxyxylulose 5-phosphate reductoisomerase
SeqlD n° 1331 LM-2699.2 From 1341879 to 1342667 unknown, similar to phosphatidate cytidylyltransferase (CDP- diglyceride synthase)
SeqlD n° 1332 LM-27.1 From 2730564 to 2731961 Unknown, similar to dGTP triphosphohydrolase
SeqlD n° 1333 LM-270.1 From 2804522 to 2805274 unknown SeqlD n° 1334 LM-2701.3 From 1779714 to 1780802 unknown, similar to putative outer surface protein
SeqlD n° 1335 LM-2702.1 From 1780824 to 1781126 unknown, similar to phosphotransferase system (PTS) lichenan- specific enzyme IIA component
SeqlD n° 1336 LM-2703.1 From 1781196 to 1781504 unknown, similar to phosphotransferase system (PTS) lichenan- specific enzyme IIB component
SeqlD n° 1337 LM-2705.2 From 1781661 to 1784339 unknown, similar to transcriptional regulator
(NifA/NtrC family)
SeqlD n° 1338 LM-2707.2 From 1784473 to 1785798 unknown, similar to ATP- dependent RNA helicases
SeqlD n° 1339 LM-2708.1 From 1785841 to 1786614 unknown SeqlD n° 1340 LM-2710.1 From 1786607 to 1787287 ubknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 1341 LM-2711.1 From 1787280 to 1787648 unknown, similar to transcriptional regulator
(GntR family)
SeqlD n° 1342 LM-2712.1 From 1787769 to 1788752 unknown, similar to hypothetical proteins
SeqlD n° 1343 LM-2713.1 From 1788812 to 1789777 unknown, similar to transcription regulators
(Lad family)
SeqlD n° 1344 LM-2714.1 From 1789825 to 1793085 unknown, some similarities to cellobiose- phosphorylase
SeqlD n° 1345 LM-2717.2 From 1793082 to 1795253 unknown, similar to beta- glucosidases
SeqlD n° 1346 LM-2718.2 From 1561978 to 1564242 Unknown, similar to protein-export membrane protein SecDF SeqlD n° 1347 LM-272.1 From 2805440 to 2807398 Unknown, similar to PTS system, fructose-specific
IIABC component
SeqlD n° 1348 LM-2720. From 1564343 to 1564633 Unknown, similar to unknown proteins
SeqlD n° 1349 LM-2721.1 From 1564776 to 1565105 Unknown, similar to unknown proteins
SeqlD n° 1350 LM-2722.1 From 1565139 to 1566278 Unknown, similar to tRNA- guanine transglycosylase
Tgt
SeqlD n° 1351 LM-2723.1 From 1566365 to 1567393 Unknown, similar to S- adenosylmethionine.tRNA ribosyltransferase- isomerase
SeqlD n° 1352 LM-2725.1 From 1567397 to 1568404 unknown, highiy similar to
Holliday junction DNA helicase RuvB
SeqlD n° 1353 LM-2726.1 From 1568420 to 1569025 unknown, highiy similar to
Holliday junction DNA helicase (ruvA)
SeqlD n° 1354 LM-2728.1 From 1569149 to 1570084 Unknown, similar to L- lactate dehydrogenase
SeqlD n° 1355 LM-2729.1 From 1570154 to 1570879 Unknown, similar to unknown proteins
SeqlD n° 1356 LM-273.1 From 2807459 to 2810107 Unknown, weakiy similar to sugar hydrolase
SeqlD n° 1357 LM-2730.1 From 1570990 to 1571838 Unknown, similar to prephenate dehydratase
PheA
SeqlD n° 1358 LM-2732.1 From 1571905 to 1573194 Unknown, conserved GTP binding protein
SeqlD n° 1359 LM-2735.1 From 1573354 to 1574847 Unknown, similar to glycerol kinase
SeqlD n° 1360 LM-2736.2 From 1574922 to 1575740 Unknown, similar to glycerol uptake facilitator
SeqlD n° 1361 LM-2737.2 From 623251 to 624423 Unknown, conserved hypothetical membrane protein
SeqlD n° 1362 LM-274.1 From 2810109 to 2811791 Unknown, similar to
Sucrose phosphorylase
SeqlD n° 1363 LM-2741.1 From 620805 to 623135 Unknown, similar to preprotein translocase
SecA subunit
SeqlD n° 1364 LM-2743.3 From 618932 to 620380 P60 extracellular protein, invasion associated protein lap
SeqlD n° 1365 LM-2746.1 From 617660 to 618844 Unknown, conserved hypothetical protein
SeqlD n° 1366 LM-2747.1 From 616973 to 617629 Unknown, weakiy similar to carboxylesterase
SeqlD n° 1367 LM-275.1 From 2811788 to 2812921 Unknown, conserved hypothetical protein SeqlD n° 1368 LM-2752.1 From 615825 to 616577 Unknown, putative conserved membrane protein
SeqlD n° 1369 LM-2753.1 From 615353 to 615811 Unknown SeqlD n° 1370 LM-2754.1 From 613836 to 615299 Unknown SeqlD n° 1371 LM-2755.2 From 612678 to 613406 Unknown, similar to transcription regulator
GntR family
SeqlD n° 1372 LM-2758.2 From 2343858 to 2345150 Unknown, similar to unknown proteins
SeqlD n° 1373 LM-2759.1 From 2345260 to 2345526 Unknown SeqlD n° 1374 LM-276.1 From 2812925 to 2813881 Unknown, similar to transcriptional regulator (Lacl family)
SeqlD n° 1375 LM-2760.1 From 2345567 to 2346088 Unknown, similar to unknown proteins SeqlD n° 1376 LM-2761.1 From 2346252 to 2346596 Unknown, hypothetical CDS SeqlD n° 1377 LM-2762.1 From 2346935 to 2347285 unknown, similar to phosphotransferase system (PTS) beta- glucoside-specific enzyme IIA
SeqlD n° 1378 LM-2763.1 From 2347276 to 2348151 Unknown, similar to unknown proteins SeqlD n° 1379 LM-2764.1 From 2348231 to 2348539 Unknown, similar to unknown proteins SeqlD n° 1380 LM-2765.1 From 2348604 to 2349356 Unknown, similar to unknown proteins SeqlD n° 1381 LM-2766.1 From 2349475 to 2350320 unknown, similar to unkown proteins SeqlD n° 1382 LM-2768.1 From 2350398 to 2351300 Unknown, similar to unknown proteins SeqlD n° 1383 LM-2769.1 From 2351384 to 2351746 Unknown, similar to unknown proteins SeqlD n° 1384 LM-2770.1 From 2351767 to 2352615 Unknown, similar to unknown proteins SeqlD n° 1385 LM-2771.1 From 2352616 to 2356323 Unknown, similar to ATP- dependent deoxyribonuclease (subunit
A)
SeqlD n° 1386 LM-2772.3 From 2356325 to 2359798 Unknown, similar to ATP- dependent deoxyribonuclease (subunit
B)
SeqlD n° 1387 LM-2774.2 From 2097176 to 2099941 isoleucyl-tRNA synthetase SeqlD n° 1388 LM-2776.1 From 2096075 to 2097064 Unknown, similar to diaminopimelate epimerase
SeqlD n° 1389 LM-2778.1 From 2095366 to 2096061 Unknown, similar to unknown proteins SeqlD n° 1390 LM-278.1 From 2813949 to 2815283 Unknown, conserved hypothetical protein similar to hypothetical hemolysin
SeqlD n° 1391 LM-2780.2 From 2091698 to 2094808 Unknown, similar to alpha- mannosidase
SeqlD n° 1392 LM-2781.3 From 2504300 to 2505157 Unknown, similar to transcription antiterminator
SeqlD n° 1393 LM-2782.1 From 2503931 to 2504236 Unknown, similar to B. subtilis YfhL protein
SeqlD n° 1394 LM-2784.1 From 2502401 to 2503804 unknown, highiy similar to glutamate decarboxylases
SeqlD n° 1395 LM-2785.1 From 2501603 to 2502361 Unknown, similar to acetylesterase
SeqlD n° 1396 LM-2788.1 From 2500179 to 2501150 Unknown, similar to B. subtilis ferrichrome ABC transporter fhuD precursor
(ferrichrome-binding protein)
SeqlD n° 1397 LM-2790.1 From 2499169 to 2500179 Unknown, similar to B. subtilis ferrichrome ABC transporter (permease)
FhuG
SeqlD n° 1398 LM-2792.1 From 2498381 to 2499172 Unknown, similar to B. subtilis ferrichrome ABC transporter (ATP-binding protein) FhuC
SeqlD n° 1399 LM-2793.1 From 2497069 to 2498238 Unknown, similar to cell division proteins RodA,
FtsW
SeqlD n° 1400 LM-2794.1 From 2495818 to 2496993 Unknown, similar to cell division proteins RodA,
FtsW
SeqlD n° 1401 LM-2795.2 From 2495309 to 2495662 Unknown, conserved hypothetical proteins
SeqlD n° 1402 LM-2796.1 From 1674961 to 1676325 unknown, highiy similar to anthranilate synthase alpha subunit
SeqlD n° 1403 LM-2797.1 From 1674359 to 1674964 unknown, highiy similar to anthranilate synthase beta subunit
SeqlD n° 1404 LM-2798.1 From 1673368 to 1674387 unknown, highiy similar to anthranilate phosphoribosyltransferase
SeqlD n° 1405 LM-2799.1 From 1672613 to 1673371 unknown, highiy similar to indol-3-glycerol phosphate synthases
SeqlD n° 1406 LM-28.1 From 2731976 to 2732455 Unknown, similar to spermidine/spermine N1- acetyl transferase
SeqlD n° 1407 LM-280.1 From 2815401 to 2816090 unknown, similar to regulatory proteins of the
SIR2 family SeqlD n° 408 LM-2801.1 From 1672008 to 1672616 phosphoribosyl anthranilate isomerase SeqlD n° 409 LM-2804.1 From 1670803 to 1672005 unknown, highiy similar to tryptophan synthase (beta subunit)
SeqlD n° 410 LM-2805.1 From 1670037 to 1670810 unknown, highiy similar to tryptophan synthase (alpha subunit)
SeqlD n° 411 LM-2806.1 From 1669529 to 1669966 unknown SeqlD n° 412 LM-2808.1 From 1667831 to 1669444 Unknown, similar to putative transporters
SeqlD n° 413 LM-2809.1 From 1666107 to 1667720 Unknown, similar to putative transporters
SeqlD n° 414 LM-2810.1 From 1665444 to 1666097 Unknown, similar to unknown proteins
SeqlD n° 415 LM-2811.2 From 1664575 to 1665405 Unknown, similar to unknown proteins
SeqlD n° 416 LM-2812.2 From 49585 to 49878 30S ribosomal protein S6 SeqlD n° 417 LM-2813.2 From 49934 to 50470 unknown, highiy similar to single-strand binding protein (SSB)
SeqlD n° 418 LM-2816.1 From 50907 to 51518 unknown SeqlD n° 419 LM-2817.1 From 51775 to 52389 Unknown, similar to
Staphylococcus AgrB protein
SeqlD n° 420 LM-2818.1 From 52630 to 53925 Unknown, similar to sensor histidine kinase (AgrC from
Staphylococcus) SeqlD n° 421 LM-2819.1 From 53944 to 54672 Unknwon, similar to 2- components response regulator protein (AgrA from Staphylococcus)
SeqlD n° 422 LM-282.1 From 2816083 to 2816373 Unknown SeqlD n° 423 LM-2820.1 From 54839 to 56812 Unknown, highiy similar to
B. subtilis YybT protein
SeqlD n° 424 LM-2822.1 From 56815 to 57261 50S ribosomal protein L9 SeqlD n° 425 LM-2823.2 From 57286 to 58638 unknown, highiy similar to replicative DNA helicases
SeqlD n° 426 LM-2826.1 From 2580470 to 2581780 Unknown, similar to cell wall binding proteins
SeqlD n° 427 LM-2827.1 From 2581900 to 2583105 peptidoglycan lytic protein
P45
SeqlD n° 428 LM-2829.1 From 2583181 to 2584065 unknown, highiy similar to cell-division protein FtsX
SeqlD n° 429 LM-283.1 From 2816363 to 2817571 Unknown, similar to drug- efflux transporters
SeqlD n° 430 LM-2831.1 From 2584055 to 2584741 unknown, highiy similar to the cell-division ATP- binding protein FtsE
SeqlD n° 431 LM-2832.1 From 2585245 to 2586108 Unknown, similar to conserved hypothetical proteins SeqlD n° 1432 LM-2833.1 From 2586114 to 2587097 unknown, highiy similar to peptide chain release factor 2
SeqlD n° 1433 LM-2834.2 From 2587300 to 2589813 translocase binding subunit (ATPase)
SeqlD n° 1434 LM-2835.2 From 1052509 to 1053429 unknown SeqlD n° 1435 LM-2836.1 From 1051695 to 1052354 unknown, similar to a bacterial K(+)-uptake system
SeqlD n° 1436 LM-2838.1 From 1051038 to 1051676 unknown, similar to two- component response regulator, in particular B. subtilis YvqC protein
SeqlD n° 1437 LM-2839.1 From 1049983 to 1051041 unknown, similar to two- component sensor histidine kinase in particular B. subtilis YvqE protein
SeqlD n° 1438 LM-284.1 From 2817688 to 2818035 unknown
SeqlD n° 1439 LM-2840.1 From 1049273 to 1049986 unknown, similar to B. subtilis YvqF protein
SeqlD n° 1440 LM-2842.1 From 1048266 to 1049138 unknown, similar to B. subtilis YitL protein
SeqlD n° 1441 LM-2843.1 From 1047490 to 1048185 unknown, similar to E. coli copper homeostasis protein CutC
SeqlD n° 1442 LM-2845.1 From 1046886 to 1047377 unknown, similar to phosphotransferase system glucose-specific enzyme IIA
SeqlD n° 1443 LM-2846.1 From 1045869 to 1046771 unknown, highiy similar to glycine betaine ABC transporters (glycine betaine-binding protein)
SeqlD n° 1444 LM-2847.1 From 1045007 to 1045855 unknown, highiy similar to glycine betaine ABC transporters (permease)
SeqlD n° 1445 LM-285.1 From 2818054 to 2818698 Unknown, similar to transaldolase
SeqlD n° 1446 LM-2851.1 From 1043821 to 1045014 unknown, highiy similar to glycine betaine ABC transporter (ATP-binding protein)
SeqlD n° 1447 LM-2853.1 From 1042605 to 1043450 unknown, similar to conserved hypothetical proteins like to B. subtilis
YkuT protein
SeqlD n° 1448 LM-2854.1 From 1041446 to 1042561 unknown, similar to N-acyl-
L-amino acid amidohydrolases
SeqlD n° 1449 LM-2856.1 From 1040672 to 1041382 unknown, similar to tetrahydrodipicolinate succinylase SeqlD n° 1450 LM-2857.1 From 1039746 to 1040624 unknown, similar to transcription regulator
(LysR family).
SeqlD n° 1451 LM-2858.1 From 1039297 to 1039749 unknown, similar to B. subtilis YkuL protein
SeqlD n° 1452 LM-2859.2 From 1038863 to 1039096 unknown, similar to B. subtilis YkuJ protein
SeqlD n° 1453 LM-286.1 From 2818841 to 2819530 Unknown, weakiy similar to transcription regulators
CRP/FNR family
SeqlD n° 1454 LM-2860.1 From 600837 to 601148 Unknown, similar to phosphorybosil-AMP- cyclohydrolase (Hisl2 protein)
SeqlD n° 1455 LM-2861.1 From 601149 to 601466 Unknown, similar to phosphoribosyl-AMP cyclohydrolase (HisI1 protein)
SeqlD n° 1456 LM-2863.1 From 601463 to 602218 unknown, highiy similar to cyclase HisF
SeqlD n° 1457 LM-2864.1 From 602208 to 602930 unknown, highiy similar to phosphoribosylformimino-
5-aminoimidazole carboxamide ribotide isomerase
SeqlD n° 1458 LM-2866.1 From 602909 to 603535 unknown, similar to amidotransferases
SeqlD n° 1459 LM-2867.1 From 603536 to 604120 imidazoleglycerol- phosphate dehydratase
SeqlD n° 1460 LM-2868.1 From 604121 to 605404 unknown, highiy similar to histidinol dehydrogenases
SeqlD n° 1461 LM-287.1 From 2819860 to 2821587 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 1462 LM-2870.1 From 605401 to 606042 Unknown, similar to ATP phosphoribosyltransferase
SeqlD n° 1463 LM-2871.2 From 606039 to 607220 histidyl-tRNA synthetase
SeqlD n° 1464 LM-2872.2 From 1645958 to 1646413 Unknown, similar to unknown proteins
SeqlD n° 1465 LM-2873.3 From 1646669 to 1647781 Unknown, similar to aminopeptidase
SeqlD n° 1466 LM-2875.1 From 1647821 to 1648366 Unknown, similar to 2-cys peroxiredoxin
SeqlD n° 1467 LM-2877.1 From 1648511 to 1649854 unknown, similar to UDP-
N-acetyl muramate-alanine ligases
SeqlD n° 1468 LM-2878.2 From 1650149 to 1652500 Unknown, similar to DNA translocase
SeqlD n° 1469 LM-288.1 From 2821641 to 2821892 Unknown SeqlD n° 1470 LM-2880.1 From 1652810 to 1653427 Unknown, similar phenylalanyl-tRNA synthetase (beta subunit) SeqlD n° 1471 LM-2881.1 From 1653433 to 1654233 Unknown, similar to unknown proteins
SeqlD n° 1472 LM-2882.1 From 1654268 to 1654579 Unknown, similar to thioredoxin
SeqlD n° 1473 LM-2883.1 From 1654902 to 1655975 Unknown, similar to aminopeptidase
SeqlD n° 1474 LM-2884.1 From 1656173 to 1656484 Unknown, similar to unknown proteins
SeqlD n° 1475 LM-2885.1 From 1656521 to 1656769 Unknown, similar to unknown proteins
SeqlD n° 1476 LM-2886.1 From 1656903 to 1657754 Unknown, similar to unknown proteins
SeqlD n° 1477 LM-2887.2 From 1657815 to 1658459 Unknown, similar to unknown proteins
SeqlD n° 1478 LM-2889.2 From 1658466 to 1659248 Unknown, similar to unknown proteins
SeqlD n° 1479 LM-289.1 From 2821912 to 2823195 seryl-trna synthetase SeqlD n° 1480 LM-2890.2 From 1307009 to 1307605 unknown, conserved hypothetical protein, similar to B. subtilis YneS protein
SeqlD n° 1481 LM-2892.1 From 1306095 to 1306967 Unknown, similar to Lactococcus lactis LacX protein
SeqlD n° 1482 LM-2894.1 From 1305781 to 1306092 Unknown, similar to B. subtilis YneQ protein
SeqlD n° 1483 LM-2895.1 From 1305390 to 1305758 Unknown, similar to B. subtilis YneP protein
SeqlD n° 1484 LM-2898.1 From 1304457 to 1305236 unknown, highiy similar to
B. subtilis CodY protein
SeqlD n° 1485 LM-29.1 From 2732589 to 2733233 Unknwon, similar to ribulose-phosphate 3- epimerase
SeqlD n° 1486 LM-290.1 From 2823530 to 2823949 Unknown, similar to B. subtilis stress protein YdaG
SeqlD n° 1487 LM-2900.1 From 1303027 to 1304436 unknown, highiy similar to
ATP-dependent Clp protease-like proteins
SeqlD n° 1488 LM-2901.1 From 1302474 to 1303013 unknown, highiy similar to beta-type subunit of the
20S proteasome
SeqlD n° 1489 LM-2902.2 From 1301551 to 1302453 unknown, similar to integrase/recombinase
SeqlD n° 1490 LM-2903.2 From 1299964 to 1301268 unknown, similar glucose inhibited division protein A
SeqlD n° 1491 LM-2907.2 From 1297823 to 1299901 unknown, highiy similar to
DNA topoisomerase I TopA
SeqlD n° 1492 LM-2908.2 From 1296691 to 1297551 Unknown, similar to polypeptide deformylase, similar to B. subtilis Smf protein SeqlD n° 493 LM-2909.1 From 1295772 to 1296557 unknown, similar to ribonuclease H rnh SeqlD n° 494 LM-291.1 From 2824079 to 2824651 Unknown, similar to glutamine amidotransferase
SeqlD n° 495 LM-2910.1 From 1294912 to 1295775 unknown, conserved hypothetical protein similar to B. subtilis YlqF protein
SeqlD n° 496 LM-2911.1 From 1294360 to 1294902 Unknown, similar to signal peptidase I SeqlD n° 497 LM-2912.2 From 1293689 to 1294258 Unknown, similar to signal peptidase I SeqlD n° 498 LM-2914.2 From 1498096 to 1500252 Unknown, similar to unknown proteins SeqlD n° 499 LM-2915.1 From 1500268 to 1501227 Unknown, similar to phosphate starvation induced protein PhoH
SeqlD n° 500 LM-2916.1 From 1501415 to 1501861 Unknown, similar to unknown proteins SeqlD n° 501 LM-2917.1 From 1502252 to 1503019 Unknown, similar to unknown proteins SeqlD n° 502 LM-2918.1 From 1503020 to 1503964 Unknown, similar to ribosomal protein L11 methyltransferase
SeqlD n° 503 LM-292.1 From 2824648 to 2826354 Unknown, similar to para- aminobenzoate synthase component I
SeqlD n° 504 LM-2921.1 From 1504037 to 1505170 heat shock protein DnaJ SeqlD n° 505 LM-2922.1 From 1505312 to 1507153 class I heat-shock protein
(molecular chaperone)
DnaK
SeqlD n° 506 LM-2924.1 From 1507187 to 1507762 heat shock protein GrpE SeqlD n° 507 LM-2925.1 From 1507804 to 1508841 transcription repressor of class I heat-shock gène
HrcA
SeqlD n° 508 LM-2926.1 From 1508988 to 1510145 unknown, highiy similar to coproporphyrinogen III oxidase
SeqlD n° 509 LM-2928.1 From 1510234 to 1511262 Unknown, similar to oxidoreductase SeqlD n° 510 LM-2929.1 From 1511375 to 1511812 Unknown, similar to transcriptional regulator
(MerR family)
SeqlD n° 511 LM-2931.3 From 1511858 to 1513684 unknown, highiy similar to
GTP-binding protein LepA SeqlD n° 512 LM-2932.2 From 2535342 to 2536268 Unknown, similar to dipeptidases SeqlD n° 513 LM-2933.1 From 2533840 to 2535183 RNA polymérase sigma-54 factor (sigma-L) SeqlD n° 514 LM-2935.1 From 2532352 to 2533398 Unknown, similar to B. subtilis CggR hypothetical transcriptional regulator SeqlD n° 1515 LM-2936.1 From 2531310 to 2532320 unknown, highiy similar to glyceraldehyde 3- phosphate dehydrogenase
SeqlD n° 1516 LM-2937.1 From 2529985 to 2531175 unknown, highiy similar to phosphoglycerate kinase
SeqlD n° 1517 LM-2939.1 From 2529184 to 2529939 unknown, highiy similar to triose phosphate isomerase
SeqlD n° 1518 LM-294.1 From 2826515 to 2828236 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 1519 LM-2942.1 From 2527650 to 2529182 unknown, highiy similar to phosphoglycerate mutase
SeqlD n° 1520 LM-2944.2 From 2526222 to 2527514 unknown, highiy similar to enolase
SeqlD n° 1521 LM-2945.1 From 2525946 to 2526119 Unknown
SeqlD n° 1522 LM-2946.2 From 2525094 to 2525813 Unknown, similar to lipolytic enzyme
SeqlD n° 1523 LM-2950.2 From 316873 to 318375 Unknown, similar to heat- shock protein htrA serine protease
SeqlD n° 1524 LM-2952.1 From 315945 to 316775 Unknown, conserved hypothetical protein similar to B. subtilis YycJ protein
SeqlD n° 1525 LM-2953.1 From 314984 to 315823 Unknown, similar to B. subtilis Yycl protein
SeqlD n° 1526 LM-2954.1 From 313659 to 314981 Unknown, similar to B. subtilis YycH protein
SeqlD n° 1527 LM-2957.1 From 311830 to 313662 Unknown, similar to two- component sensor histidine kinase
SeqlD n° 1528 LM-2958.1 From 310932 to 311645 Unknown, similar to two- component response regulator
SeqlD n° 1529 LM-2959.2 From 309538 to 310719 Unknown, similar to aminotransferase
SeqlD n° 1530 LM-296.1 From 2828236 to 2830008 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 1531 LM-2960.3 From 2692097 to 2693041 unknown, highiy similar to
RNA polymérase (alpha subunit)
SeqlD n° 1532 LM-2962.2 From 2693151 to 2693540 ribosomal protein S11
SeqlD n° 1533 LM-2964.2 From 2693563 to 2693928 ribosomal protein S13
SeqlD n° 1534 LM-2965.1 From 2694164 to 2694382 unknown, highiy similar to initiation factor IF-I
SeqlD n° 1535 LM-2967.1 From 2694769 to 2695416 unknown, highiy similar to adenylate kinases
SeqlD n° 1536 LM-2968.1 From 2695476 to 2696771 unknown, highiy similar to preprotein translocase subunit
SeqlD n° 1537 LM-297.1 From 2830284 to 2830523 Unknown SeqlD n° 1538 LM-2970.1 From 2696771 to 2697211 ribosomal protein L15
SeqlD n° 1539 LM-2972.1 From 2697463 to 2697966 ribosomal protein S5
SeqlD n° 1540 LM-2973.1 From 2697988 to 2698347 ribosomal protein L18
SeqlD n° 1541 LM-2974.1 From 2698387 to 2698923 ribosomal protein L6
SeqlD n° 1542 LM-2975.1 From 2698954 to 2699352 ribosomal protein S8
SeqlD n° 1543 LM-2977.1 From 2699600 to 2700139 ribosomal protein L5
SeqlD n° 1544 LM-2979.1 From 2700166 to 2700477 ribosomal protein L24
SeqlD n° 1545 LM-298.1 From 2830542 to 2831879 Unknown, similar to D- alanyl-D-alanine carboxypeptidase
(penicillin-binding protein 5)
SeqlD n° 1546 LM-2981.1 From 2700515 to 2700883 ribosomal protein L14
SeqlD n° 1547 LM-2983.1 From 2701435 to 2701869 ribosomal protein L16
SeqlD n° 1548 LM-2984.1 From 2701872 to 2702528 ribosomal protein S3
SeqlD n° 1549 LM-2985.2 From 2702532 to 2702888 ribosomal protein L22
SeqlD n° 1550 LM-2986.2 From 2702909 to 2703187 ribosomal protein S19
SeqlD n° 1551 LM-2990.2 From 1593100 to 1594407 unknown, highiy similar to glutamyl-tRNA reductase
SeqlD n° 1552 LM-2991.1 From 1592167 to 1593096 unknown, highiy similar to porphobilinogen deaminases
(hydroxymethylbilane synthase)
SeqlD n° 1553 LM-2992.1 From 1591448 to 1592170 Unknown, similar to uroporphyrinogen III cosynthase (HemD)
SeqlD n° 1554 LM-2993.1 From 1590477 to 1591451 unknown, highiy similar to delta-aminolevulinic acid dehydratases
(porphobilinogen synthase)
SeqlD n° 1555 LM-2995.1 From 1589175 to 1590464 unknown, highiy similar to glutamate-1-semialdehyde
2,1-aminotransferases
SeqlD n° 1556 LM-2997.1 From 1586201 to 1588852 valyl-tRNA synthetase
SeqlD n° 1557 LM-2999.2 From 1584850 to 1586139 unknown, similar to Folyl- polyglutamate synthetase
SeqlD n° 1558 LM-3.1 From 2712931 to 2713365 Unknown
SeqlD n° 1559 LM-3000.1 From 1583928 to 1584638 Unknown, similar to B. subtilis late compétence protein ComC (type IV prepilin peptidase)
SeqlD n° 1560 LM-3001.1 From 1583242 to 1583916 Unknown, similar to DNA repair protein RadC
SeqlD n° 1561 LM-3004.1 From 1581777 to 1582790 unknown, similar to cell- shape determining protein
MreB
SeqlD n° 1562 LM-3005.1 From 1580804 to 1581691 unkown, similar to cell- shape determining protein
MreC
SeqlD n° 1563 LM-3006.1 From 1580283 to 1580801 unknown, similar to cell- shape determining protein
MreD SeqlD n° 1564 LM-3007.2 From 1579428 to 1580105 unknown, similar to cell- division inhibition (septum placement) protein MinC
SeqlD n° 1565 LM-301.1 From 2832058 to 2833725 Unknown, similar to acylase and diesterase SeqlD n° 1566 LM-3010.1 From 1149406 to 1149720 unknown, highiy similar to
TN916 ORF23 SeqlD n° 1567 LM-3011.1 From 1149011 to 1149385 unknown, highiy similar to
TN916 ORF22 SeqlD n° 1568 LM-3012.1 From 1147603 to 1149003 unknown, highiy similar to
TN916 ORF21 SeqlD n° 1569 LM-3013.1 From 1146228 to 1147412 unknown, highiy similar to
TN916 ORF20 SeqlD n° 1570 LM-3014.1 From 1145941 to 1146231 unknown, similar to unknown proteins SeqlD n° 1571 LM-3016.1 From 1145211 to 1145711 unknown, highiy similar to
TN916 ORF18 SeqlD n° 1572 LM-3017.1 From 1144533 to 1144928 unknown, highiy similar to
TN916 ORF17 SeqlD n° 1573 LM-3018.1 From 1142099 to 1144549 unknown, highiy similar to
TN916 ORF16 SeqlD n° 1574 LM-3020.1 From 1139940 to 1142099 unknown, highiy similar to
TN916 ORF15 SeqlD n° 1575 LM-3022.1 From 1138930 to 1139940 unknown, highiy similar to
TN916 ORF14 and to L. monocytogenes P60 protein
SeqlD n° 1576 LM-3023.1 From 1137997 to 1138914 unknown, highiy similar to
TN916 ORF13
SeqlD n° 1577 LM-3024.1 From 1137533 to 1137868 unknown, similar to cadmium efflux system accessory proteins
SeqlD n° 1578 LM-3025.3 From 1254459 to 1254722 unknown, similar to B. subtilis YshA protein
SeqlD n° 1579 LM-3027.3 From 1253379 to 1254311 unknown, similar to B. subtilis ribonuclease Hlll
SeqlD n° 1580 LM-3028.1 From 1252667 to 1253341 Unknown, similar to uracil-
DNA glycosylase
SeqlD n° 1581 LM-3029.1 From 1249360 to 1252560 unknown; similar to transporter, (to B. subtilis
YdgH protein)
SeqlD n° 1582 LM-3030.1 From 1248892 to 1249344 unknown; similar to transcriptional regulator
(MarR family).
SeqlD n° 1583 LM-3031.2 From 1245384 to 1248794 unknown, similar to différent proteins
SeqlD n° 1584 LM-3032.2 From 1244669 to 1245370 unknown, similar to ABC transporter, ATP-binding proteins
SeqlD n° 1585 LM-3035.2 From 1608166 to 1609923 unknown, highiy similar to pyruvate kinases SeqlD n° 1586 LM-3036.1 From 1607671 to 1608048 Unknown, similar to unknown proteins
SeqlD n° 1587 LM-3037.1 From 1607053 to 1607514 Unknown, similar to unknown proteins
SeqlD n° 1588 LM-3038.1 From 1605895 to 1607016 unknown, highiy similar to citrate synthase subunit II
SeqlD n° 1589 LM-3039.1 From 1604613 to 1605875 unknown, highiy similar to isocitrate dehyrogenases
SeqlD n° 1590 LM-304.1 From 2833834 to 2835987 Unknown, similar to DNA topoisomerase III
SeqlD n° 1591 LM-3041.1 From 1601820 to 1604447 DNA polymérase I SeqlD n° 1592 LM-3042.1 From 1600975 to 1601796 unknown, highiy similar to formamidopyrimidine-DNA glycosylases
SeqlD n° 1593 LM-3043.2 From 1600356 to 1600958 Unknown, similar to unknown proteins
SeqlD n° 1594 LM-3044.2 From 1875574 to 1876560 Unknown, similar to FtsY of E. coli and SRP receptor alpha-subunit
SeqlD n° 1595 LM-3049.1 From 1876575 to 1880135 Unknown, similar to Smc protein essential for chromosome condensation and partition
SeqlD n" 1596 LM-3050.1 From 1880158 to 1880847 . Unknown, similar to ribonuclease III
SeqlD n° 1597 LM-3053.1 From 1881381 to 1882124 Unknown, similar to 3- ketoacyl-acyl carrier protein reductase
SeqlD n° 1598 LM-3054.1 From 1882128 to 1883069 Unknown, similar to malonyl CoA-acyl carrier protein transacylase
SeqlD n° 1599 LM-3056.1 From 1883062 to 1884075 Unknown, similar to pIsX protein involved in fatty acid/phospholipid synthesis
SeqlD n° 1600 LM-3058.2 From 1884096 to 1884665 Unknown, similar to unknown proteins
SeqlD n° 1601 LM-3059.2 From 817914 to 818762 Unknown, similar to conserved hypothetical proteins
SeqlD n° 1602 LM-3062.1 From 813451 to 817878 Unknown SeqlD n° 1603 LM-3065.3 From 811813 to 813189 Unknown, similar to amino acid transporter
SeqlD n° 1604 LM-3066.2 From 2159224 to 2159604 Unknown SeqlD n° 1605 LM-3067.1 From 2157949 to 2159094 unknown SeqlD n° 1606 LM-3068.1 From 2157383 to 2157844 Unknown, similar to unknown proteins
SeqlD n° 1607 LM-3069.1 From 2156694 to 2157386 Unknown, similar to glycoprotease
SeqlD n° 1608 LM-307.1 From 2836011 to 2837783 Unknown, similar to ATP- dependent DNA helicases SeqlD n° 1609 LM-3070.1 From 2156242 to 2156697 Unknown, similar to ribosomal protein alanine acetyltransferase
SeqlD n° 1610 LM-3072.1 From 2155223 to 2156245 Unknown, similar to glycoprotein endopeptidase
SeqlD n° 1611 LM-3073.1 From 2153720 to 2154679 Unknown, similar to unknown proteins
SeqlD n° 1612 LM-3074.1 From 2151744 to 2153696 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 1613 LM-3075.1 From 2150786 to 2151433 Unknown, similar to a putative DNA binding proteins
SeqlD n° 1614 LM-3076.2 From 2149868 to 2150554 Unknown, similar to unknown proteins
SeqlD n° 1615 LM-3077.2 From 992978 to 993886 unknown, similar to proteases
SeqlD n° 1616 LM-3080.1 From 993900 to 995126 unknown, similar to proteases
SeqlD n° 1617 LM-3081.1 From 995210 to 995767 unknown, Listeria epitope
LemA
SeqlD n° 1618 LM-3083.1 From 995790 to 996704 unknown, similar to putative heat shock protein
HtpX, Listeria epitope
LemB
SeqlD n° 1619 LM-3084.1 From 996739 to 997557 unknown, similar to B. subtilis YjbH protein
SeqlD n° 1620 LM-3085.1 From 997795 to 998379 unknown, similar to B. subtilis YjbK protein
SeqlD n° 1621 LM-3086.2 From 998396 to 998863 unknown
SeqlD n° 1622 LM-3087.2 From 999022 to 999690 unknown, similar to B. subtilis YjbM protein
SeqlD n° 1623 LM-3088.1 From 999722 to 1000516 unknown, similar to conserved hypothetical proteins like to B. subtilis
YjbN protein
SeqlD n° 1624 LM-3089.1 From 1000535 to 1001425 unknown, similar to ribosomal large subunit pseudouridine synthetase
SeqlD n° 1625 LM-309.2 From 2837944 to 2839410 Unknown, similar to inosine-monophosphate dehydrogenase
SeqlD n° 1626 LM-3090.1 From 1001503 to 1002291 unknown, similar to enoyl- acyl-carrier protein reductase
SeqlD n° 1627 LM-3091.1 From 1002319 to 1003593 DltD protein for D-alanine esterification of lipoteichoic acid and wall teichoic acid SeqlD n° 1628 LM-3092.1 From 1003855 to 1005039 DltB protein for D-alanine esterification of lipoteichoic acid and wall teichoic acid
SeqlD n° 1629 LM-3093.2 From 1005036 to 1006568 D-alanine-activating enzyme (dae), D-alanine-
D-alanyl carrier protein ligase (dcl)
SeqlD n° 1630 LM-3095.2 From 1364031 to 1364570 unknown, similar to 5- formyltetrahydrofolate cyclo-ligase
SeqlD n° 1631 LM-3097.1 From 1364669 to 1366207 unknown similar to B. subtilis yqgP
SeqlD n° 1632 LM-3098.1 From 1366457 to 1367425 Unknown, similar to glucose kinase
SeqlD n° 1633 LM-3099.1 From 1367559 to 1368650 unknown, similar to B. subtilis YqgU protein
SeqlD n° 1634 LM-3100.1 From 1368668 to 1368985 Unknown, weakiy similar to
B. subtilis comG operon protein 7 (comGG)
SeqlD n° 1635 LM-3101.1 From 1368982 to 1369449 Unknown, similar to B. subtilis comG operon protein 6
SeqlD n° 1636 LM-3102.1 From 1369412 to 1369696 Unknown, similar to comG operon protein 5 (comGE)
SeqlD n° 1637 LM-3103.1 From 1369683 to 1370111 Unknown, similar to comG operon protein 4 (comGD)
SeqlD n° 1638 LM-3104.1 From 1370108 to 1370431 Unknown, similar to B. subtilis comG operon protein 3
SeqlD n° 1639 LM-3105.1 From 1370445 to 1371476 Unknown, similar to B. subtilis comG operon protein 2
SeqlD n° 1640 LM-3106.1 From 1371454 to 1372476 Unknown, similar to B. subtilis comG operon protein 1
SeqlD n° 1641 LM-3107.1 From 1373015 to 1374103 Unknown, similar to aminomethyltransferase
SeqlD n° 1642 LM-3110.3 From 1374119 to 1375465 Unknown, similar to glycine dehydrogenase (decarboxylating) subunit 1
SeqlD n° 1643 LM-3111.1 From 1375462 to 1376928 Unknown, similar to glycine dehydrogenase
(decarboxylating) subunit 2
SeqlD n° 1644 LM-3112.1 From 1376968 to 1377348 unknown
SeqlD n° 1645 LM-3113.1 From 1377410 to 1377682 Unknown
SeqlD n° 1646 LM-3114.2 From 1377695 to 1378660 Unknown, similar to B. subtilis YqhQ protein
SeqlD n° 1647 LM-3115.3 From 1962698 to 1963243 unknown, similar to conserved hypothetical proteins SeqlD n° 648 LM-3116.2 From 1962225 to 1962566 unknown, similar to hypothetical proteins SeqlD n° 649 LM-3119.1 From 1960496 to 1961644 unknown, similar to conserved hypothetical proteins
SeqlD n° 650 LM-312.1 From 130652 to 131380 Unknwon, similar to autolysin: N- acetylmuramoyl-L-alanine amidase
SeqlD n° 651 LM-3120.1 From 1958968 to 1960476 unknown, similar to probable thermostable carboxypeptidases
SeqlD n° 652 LM-3121.1 From 1958162 to 1958740 unknown, similar to xanthine phosphoribosyltransferase
SeqlD n° 653 LM-3124.1 From 1956848 to 1958155 unknown, similar to probable permeases SeqlD n° 654 LM-3126.1 From 1955598 to 1956656 unknown, similar to chitinases SeqlD n° 655 LM-3127.2 From 1955205 to 1955474 unknown, similar to ribosomal protein S14 SeqlD n° 656 LM-3128.3 From 1954255 to 1955127 unknown, similar to 5 -3 exonuclease SeqlD n° 657 LM-3129.1 From 248555 to 249013 Unknown, highiy similar to transcription repressor of class III stress gènes
(CtsR)
SeqlD n° 658 LM-313.1 From 130249 to 130671 Unknwon, similar to a protein from Bacteriophage phi-105 (ORF 45)
SeqlD n° 659 LM-3130.1 From 249026 to 249544 Unknown, similar to B. subtilis YacH protein SeqlD n° 660 LM-3131.1 From 249541 to 250563 Unknwon, similar to arginine kinase SeqlD n° 661 LM-3135.1 From 250592 to 253054 endopeptidase Clp ATP- binding chain C SeqlD n° 662 LM-3136.2 From 253200 to 254573 unknown, similar to DNA repair protein Sms SeqlD n° 663 LM-3139.2 From 254707 to 255780 Unknown, highiy similar to
B. subtilis YacL protein SeqlD n° 664 LM-314.1 From 129694 to 130230 Unknwon, weakiy similar to protein gp20 from
Bacteriophage A118
SeqlD n° 665 LM-3140.2 From 255800 to 256498 Unknown, similar to nucleotidylyl transferase; pyrophosphorylase
SeqlD n° 666 LM-3141.2 From 348586 to 349065 Unknown SeqlD n° 667 LM-3142.2 From 349326 to 350228 Unknown, similar to transcriptional regulators
SeqlD n° 668 LM-3143. From 350392 to 351264 Unknown, similar to transcriptional regulators
SeqlD n° 669 LM-3147.1 From 355643 to 356323 Unknown SeqlD n° 1670 LM-3149.2 From 2602249 to 2602683 Unknwon, similar to hydroxymyristoyl-(acyl carrier protein) dehydratase
SeqlD n° 1671 LM-315.1 From 129218 to 129697 Unknwon SeqlD n° 1672 LM-3150.1 From 2601632 to 2601997 Unknown, similar to single- strand DNA-binding protein
SeqlD n° 1673 LM-3151.1 From 2600408 to 2601241 Unknwon, similar to hypothetical cell wall binding protein from B. subtilis
SeqlD n° 1674 LM-3152.1 From 2599548 to 2600282 Unknown, similar to B. subtilis TagA protein involved in polyglycerol phosphate biosynthesis
SeqlD n° 1675 LM-3153.1 From 2598423 to 2599547 Unknwon, similar to B. subtilis O- succinylbenzoate-CoA synthase (MenC)
SeqlD n° 1676 LM-3154.1 From 2597363 to 2598415 Unknown, similar to B. subtilis TagO teichoic acid linkage unit synthesis protein
SeqlD n° 1677 LM-3155.1 From 2596266 to 2597324 Unknown, similar to B. subtilis putative transcriptional regulator
LytR
SeqlD n° 1678 LM-3156.1 From 2595485 to 2596090 unknwon SeqlD n° 1679 LM-3157.1 From 2594908 to 2595543 Unknown, similar to conserved hypothetical proteins
SeqlD n° 1680 LM-3158.1 From 2593837 to 2594523 Unknown, similar to B. subtilis two-component response regulator DegU
SeqlD n° 1681 LM-3159.1 From 2592965 to 2593816 Unknown, similar to B. subtilis YvîA (DegV) protein
SeqlD n° 1682 LM-316.1 From 128628 to 129203 unknown
SeqlD n° 1683 LM-3162.1 From 2591451 to 2592770 unknown, similar to late compétence protein comFA
SeqlD n° 1684 LM-3163.2 From 2590802 to 2591458 unknown, similar to late compétence protein comFC
SeqlD n° 1685 LM-3164.2 From 2590044 to 2590607 Unknown, similar to conserved hypothetical proteins like to B. subtilis
YvyD protein
SeqlD n° 1686 LM-3167.1 From 2020738 to 2021256 Unknown, similar to similar to acyl-CoA hydrolase SeqlD n° 1687 LM-3168.1 From 2021272 to 2023062 Unknown, similar to two- component sensor histidine kinase (ResE) SeqlD n° 1688 LM-3169.1 From 2023163 to 2023879 Unknown, similar to two- component response regulator (ResD)
SeqlD n° 1689 LM-317.1 From 128314 to 128613 Unknown
SeqlD n° 1690 LM-3171.1 From 2024062 to 2024796 Unknown, similar to unknown proteins
SeqlD nD 1691 LM-3172. From 2024799 to 2025395 Unknown, similar to unknown proteins
SeqlD n° 1692 LM-3174.1 From 2025392 to 2026141 Unknown, similar to unknown proteins
SeqlD n° 1693 LM-3177.1 From 2026157 to 2027467 Unknown, similar to diaminopimelate decarboxylase
SeqlD n° 1694 LM-3178.1 From 2027648 to 2028466 Unknown, similar to purine- nucleoside phosphorylase
SeqlD n° 1695 LM-3179.2 From 2028485 to 2029669 Unknown, similar to ' phosphopentomutase
SeqlD n° 1696 LM-3181.2 From 2029698 to 2030591 Unknown, similar to integrase/recombinase
SeqlD n° 1697 LM-3184.2 From 635317 to 636423 Unknown, similar to homoserine O- acetyltransferase
SeqlD n° 1698 LM-3186.1 From 636440 to 637717 Unknown, similar to O- acetylhomoserine sulfhydrylase
SeqlD n° 1699 LM-3188.1 From 638165 to 638692 Unknown, similar to unknown proteins
SeqlD n° 1700 LM-3189.1 From 638781 to 639482 Unknown, similar to transcription regulator
CRP/FNR family
SeqlD n° 1701 LM-319.1 From 127188 to 128324 Unknown, similar to protein gplδ from Bacteriophage
A118
SeqlD n° 1702 LM-3190.2 From 639558 to 640106 Unknown, similar to proteins involved in biotin metabolism (BioY)
SeqlD n° 1703 LM-3191.2 From 1537815 to 1538501 Unknown, similar to two- component response regulators
SeqlD n° 1704 LM-3192.1 From 1538498 to 1539937 Unknown, similar to two- component sensor histidine kinase
SeqlD n° 1705 LM-3194.1 From 1539979 to 1542375 Unknown, similar to exodeoxyribonuclease V
SeqlD n° 1706 LM-3195.1 From 1542403 to 1543041 Unknown, similar to unknown proteins
SeqlD n° 1707 LM-3196.1 From 1543082 to 1543822 Unknown, similar to unknown proteins
SeqlD n° 1708 LM-3198.1 From 1543940 to 1545055 Unknown, similar to putative tRNA (5- methylaminomethyl-2- thiouridylate)- methyltransferase
SeqlD n° 709 LM-32.1 From 2733230 to 2735245 Unknown, similar to transketolase SeqlD n° 710 LM-3200.2 From 1545074 to 1546222 Unknown, similar to iron- sulfur cofactor synthesis protein
SeqlD n° 711 LM-3201.2 From 1527422 to 1527904 Unknown, similar to transcription elongation factor GreA
SeqlD n° 712 LM-3203.2 From 1526748 to 1527371 Unknown, similar to unknown proteins SeqlD n° 713 LM-3204.1 From 1525997 to 1526698 Unknown, similar to 5 - methylthioadenosine/S- adenosylhomocysteine nucleosidase
SeqlD n° 714 LM-3205.1 From 1524154 to 1525962 Unknown, similar to oligopeptidase SeqlD n° 715 LM-3206.2 From 1523471 to 1523992 Unknown, similar to unknown proteins SeqlD n° 716 LM-3208.2 From 1522374 to 1523474 Unknown, similar to unknown proteins SeqlD n° 717 LM-3209.1 From 1521533 to 1522354 Unknown, similar to shikimate 5- dehydrogenase (AroD)
SeqlD n° 718 LM-321.1 From 126360 to 127178 Unknown, similar to phage proteins
SeqlD n° 719 LM-3210.1 From 1521243 to 1521533 Unknown, similar to unknown proteins
SeqlD n° 720 LM-3211.1 From 1520659 to 1521225 Unknown, similar to unknown proteins
SeqlD n° 721 LM-3212.1 From 1520097 to 1520672 Unknown, similar to unknown proteins
SeqlD n° 722 LM-3213.1 From 1519736 to 1520092 Unknown SeqlD n° 723 LM-3214.1 From 1518950 to 1519681 Unknown, similar to unknown proteins
SeqlD n° 724 LM-3215.1 From 1518245 to 1518847 Unknown, similar to intégral membrane protein
ComEA
SeqlD n° 725 LM-3216.1 From 1517613 to 1518173 Unknown, similar to B. subtilis ComEB protein SeqlD n° 726 LM-3217.2 From 1515356 to 1517578 Unknown, similar to putative intégral membrane protein ComEC specifically required for DNA uptake but not for binding
SeqlD n° 1727 LM-3218.2 From 1418803 to 1420095 Unknown, similar to putative proteases SeqlD n° 1728 LM-3220.1 From 1417735 to 1418685 Unknown, similar to sugar ABC transporter, permease protein
SeqlD n° 1729 LM-3221.1 From 1416686 to 1417738 Unknown, similar to permease proteins
SeqlD n° 1730 LM-3223.1 From 1415152 to 1416693 Unknown, similar to sugar ABC transporter, ATP- binding protein
SeqlD n° 1731 LM-3226.3 From 1413646 to 1414719 Unknown, CD4+ T cell- stimulating antigen, lipoprotein
SeqlD n° 1732 LM-3228.3 From 1412455 to 1413294 Unknown, similar to pyrroline-5-carboxylate reductase
SeqlD n° 1733 LM-3231.1 From 1410148 to 1412421 Unknown, similar to DNA translocase
SeqlD n° 1734 LM-3234.2 From 843311 to 843724 Unknown, similar to E. coli
PhnB protein
SeqlD n° 1735 LM-3235.1 From 842781 to 843287 Unknown, similar to B. subtilis regulatory protein
PaiA
SeqlD n° 1736 LM-3237.1 From 842313 to 842765 unknown, similar to transcription regulators
SeqlD n° 1737 LM-3239.1 From 841157 to 842086 unknown, similar to oxidoreductases
SeqlD n° 1738 LM-324.1 From 124495 to 126363 unknown, similar to bacteriophage minortail proteins
SeqlD n° 1739 LM-3241.1 From 840200 to 841072 unknown, similar to fructokinases
SeqlD n° 1740 LM-3243.3 From 839520 to 840062 unknown
SeqlD n° 1741 LM-3244.3 From 838751 to 839452 unknown, similar to carbonic anhydrase
SeqlD n° 1742 LM-3246.1 From 837548 to 838621 Unknown, similar to spermidine/putrescine- binding protein
SeqlD n° 1743 LM-3248.1 From 836745 to 837551 unknown, similar to spermidine/putrescine ABC transporter, permease protein
SeqlD n° 1744 LM-3249.1 From 835939 to 836748 unknown, similar to spermidine/putrescine ABC transporter, permease protein
SeqlD n° 1745 LM-3250.1 From 834845 to 835939 unknown, similar to spermidine/putrescine ABC transporter, ATP-binding protein
SeqlD n° 1746 LM-3251.2 From 834287 to 834829 unknown, similar to transcription regulator SeqlD n° 747 LM-3252.2 From 1724612 to 1725565 unknown, similar to ABC transporter and adhésion proteins
SeqlD n° 748 LM-3254.1 From 1725584 to 1726993 similar to O- succinylbenzoic acid-CoA ligase
SeqlD n° 749 LM-3255.1 From 1727009 to 1727827 unknown, similar to dihydroxynapthoic acid synthetase
SeqlD n° 750 LM-3256.1 From 1727824 to 1728651 unknown, similar to prolyl aminopetidases SeqlD n° 751 LM-3258.1 From 1728653 to 1730395 unknown, similar to 2- succinyl-6-hydroxy-2,4- cyclohexadiene-1- carboxylate synthase / 2- oxoglutarate decarboxylase
SeqlD n° 1752 LM-326.1 From 124104 to 124508 unknown
SeqlD n° 1753 LM-3260.2 From 1730392 to 1731780 unknown, similar to menaquinone-specific isochorismate synthase
SeqlD n° 1754 LM-3261.2 From 2308741 to 2311464 Unknown, similar to unknown proteins
SeqlD n° 1755 LM-3262.1 From 2311461 to 2312696 Unknown, similar to unknown proteins
SeqlD n° 1756 LM-3263.1 From 2312839 to 2313192 Unknown, similar to unknown proteins
SeqlD n° 1757 LM-3265.1 From 2313274 to 2314407 Unknown, similar to unknown proteins
SeqlD n° 1758 LM-3266.1 From 2314511 to 2315878 Unknown, similar to fumarate hydratase
SeqlD n° 1759 LM-3267.1 From 2315948 to 2316742 Unknown, similar to unknown proteins
SeqlD n° 1760 LM-3268.1 From 2316739 to 2317644 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 1761 LM-3269.2 From 2317950 to 2320094 Unknown, similar to penicillin-binding protein
SeqlD n° 1762 LM-3274.2 From 558526 to 560187 Unknown, similar to putative sulfate transporter
SeqlD n° 1763 LM-3275.1 From 558037 to 558480 Unknown, similar to B. subtilis YybC protein
SeqlD n° 1764 LM-3276.1 From 557178 to 557930 Unknown, similar to transcription regulator
SeqlD n° 1765 LM-3278.1 From 555660 to 556976 Unknown, similar to 6- phospho-beta-glucosidase
SeqlD n° 1766 LM-328.1 From 123760 to 124062 Unknwon
SeqlD n° 1767 LM-3281.1 From 554624 to 555628 Unknown, similar to transcription regulator
SeqlD n° 1768 LM-3283.2 From 553078 to 554493 Unknown, similar to multidrug résistance protein 129
SeqlD n° 1769 LM-3284.1 From 514004 to 514381 Unknown, putative secreted protein
SeqlD n° 1770 LM-3285.1 From 514681 to 515058 Unknown, putative secreted protein
SeqlD n° 1771 LM-3286.1 From 515391 to 515750 Unknown, putative secreted protein
SeqlD n° 1772 LM-3288.1 From 516076 to 516636 Unknown
SeqlD n° 1773 LM-329.1 From 123200 to 123712 antigen A
SeqlD n° 1774 LM-3290.1 From 516746 to 518446 Unknown, similar to unknown proteins
SeqlD n° 1775 LM-3292.1 From 518597 to 519700 Unknown, similar to conserved hypothetical proteins, highiy similar to B. subtilis YloN protein
SeqlD n° 1776 LM-3293.1 From 519789 to 520268 Unknown
SeqlD n° 1777 LM-3294.2 From 520426 to 520791 Unknown
SeqlD n° 1778 LM-3296.2 From 1561538 to 1561882 Unknown, similar to unknown proteins
SeqlD n° 1779 LM-3298.1 From 1559081 to 1561432 Unknown, similar to single- stranded-DNA-specific exonuclease (RecJ)
SeqlD n° 1780 LM-3299.1 From 1558570 to 1559091 unknown, similar to adenine phosphoribosyltransferase
SeqlD n° 1781 LM-330.1 From 122798 to 123187 antigen B
SeqlD π° 1782 LM-3301.1 From 1556148 to 1558364 unknown, similar to
(p)ppGpp synthetase
SeqlD n° 1783 LM-3302.1 From 1555680 to 1556132 Unknown, similar to unknown proteins
SeqlD n° 1784 LM-3303.2 From 1554360 to 1555643 Unknown, similar to N- acetylmuramoyl-L-alanine amidase
SeqlD n° 1785 LM-3305.2 From 2279524 to 2279919 Unknown, similar to unknown proteins
SeqlD n° 1786 LM-3306.1 From 2280238 to 2281206 Unknown, similar to oligopeptide ABC transporter (ATP-binding protein)
SeqlD n° 1787 LM-3307.1 From 2281203 to 2282279 Unknown, similar to oligopeptide ABC transporter (ATP-binding protein)
SeqlD n° 1788 LM-3309.1 From 2282297 to 2283331 Unknown, similar to oligopeptide ABC transporter (permease)
SeqlD n° 1789 LM-331.1 From 122104 to 122520 unknown, similar to Antigen
C
SeqlD n° 1790 LM-3310.1 From 2283331 to 2284260 Unknown, similar to oligopeptide ABC transporter (permease)
SeqlD n° 1791 LM-3312.2 From 2284539 to 2286215 Unknown, similar to pheromone binding protein 01/77335
130
SeqlD n° 1792 LM-3315.1 From 1528098 to 1528727 unknown, similar to Uridine kinase
SeqlD n° 1793 LM-3316.1 From 1528724 to 1529377 Unknown, similar to O- methyltransferase
SeqlD n° 1794 LM-3319.1 From 1529454 to 1530524 Unknown, similar to unknown proteins
SeqlD n° 1795 LM-332.1 From 121664 to 122092 unknown, similar to Antigen
D
SeqlD n° 1796 LM-3320.1 From 1530727 to 1531353 Unknown, similar to unknown proteins
SeqlD n° 1797 LM-3321.1 From 1531398 to 1531700 Unknown, similar to unknown proteins
SeqlD n° 1798 LM-3322.1 From 1531716 to 1532132 Unknown, similar to unknown proteins
SeqlD n° 1799 LM-3323.1 From 1532129 to 1532401 Unknown
SeqlD n° 1800 LM-3326.1 From 1532493 to 1535132 alanyl-tRNA synthetase
SeqlD n° 1801 LM-3327.1 From 1535439 to 1536134 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 1802 LM-3329.2 From 1536143 to 1537648 Unknown, similar to transporter
SeqlD n° 1803 LM-333.1 From 121112 to 121447 Unknwon, similar to putative repressor C1 from lactococcal bacteriophage
Tuc2009
SeqlD n° 1804 LM-3330.2 From 512291 to 513268 Unknown, similar to oxetanocin A résistance protein oxrB
SeqlD n° 1805 LM-3331.1 From 511966 to 512208 Unknown
SeqlD n° 1806 LM-3332.1 From 511215 to 511562 Unknown
SeqlD n° 1807 LM-3333.1 From 510163 to 511212 Unknown
SeqlD n° 1808 LM-3334.1 From 509400 to 510287 Unknown
SeqlD n° 1809 LM-3335.2 From 507723 to 508733 Unknown
SeqlD n° 1810 LM-3336.3 From 506368 to 506997 Unknown, weakiy similar to site-specific DNA- methyltransferase
SeqlD n° 1811 LM-3337.3 From 505351 to 506223 Unknown
SeqlD n° 1812 LM-3338.2 From 1487699 to 1489579 DNA primase
SeqlD n° 1813 LM-3339.1 From 1489599 to 1490039 Unknown, similar to unknown proteins
SeqlD n° 1814 LM-334.1 From 120654 to 121106 Unknwon, similar to protein gp35 from Bacteriophage
A118
SeqlD n° 1815 LM-3340.1 From 1490229 to 1491053 Unknown, similar to unknown protein
SeqlD n° 1816 LM-3341.1 From 1491087 to 1493153 Unknown, similar to glycyl- tRNA synthetase beta chain
SeqlD n° 1817 LM-3342.1 From 1493146 to 1494036 Unknown, similar to glycyl- tRNA synthetase alpha chain SeqlD n° 1818 LM-3343.1 From 1494317 to 1495084 Unknown, similar to B. subtilis RecO protein involved in DNA repair and homologous recombination
SeqlD n° 1819 LM-3344.1 From 1495221 to 1495850 Unknown
SeqlD n° 1820 LM-3345.1 From 1495899 to 1496804 Unknown, similar to GTP binding proteins
SeqlD n° 1821 LM-3347.1 From 1496801 to 1497196 Unknown, similar to cytidine deaminase
SeqlD n° 1822 LM-3348.1 From 1497221 to 1497616 Unknown, similar to diacylglycerol kinase
SeqlD n° 1823 LM-3349.1 From 1497594 to 1498079 Unknown, similar to unknown proteins
SeqlD n° 1824 LM-335.1 From 119740 to 120435 Unknown, weakiy similar to transcription regulators,
Fnr/Crp family
SeqlD n° 1825 LM-3355.1 From 2012582 to 2013727 Unknown, similar to similar to ribosomal protein S1 like protein
SeqlD n° 1826 LM-3356.1 From 2014086 to 2014760 Unknown, similar to cytidylate kinase
SeqlD n° 1827 LM-3357.1 From 2014776 to 2015738 Unknown, similar to asparaginase
SeqlD n° 1828 LM-3359.1 From 2015819 to 2016538 Unknown, similar to unknown proteins
SeqlD n° 1829 LM-336.1 From 119063 to 119770 Unknwon SeqlD n° 1830 LM-3360.1 From 2016858 to 2018261 Unknown, similar to ATP- dependent DNA helicase
SeqlD n° 1831 LM-3361.2 From 2018258 to 2019265 Unknown, similar to unknown proteins
SeqlD n° 1832 LM-3362.2 From 2019420 to 2019644 Unknown, similar to ferredoxin
SeqlD n° 1833 LM-3363.3 From 2019686 to 2020297 Unknown, similar to unknown protein
SeqlD n° 1834 LM-3364.2 From 1867802 to 1869460 Unknown, similar to unknown protein
SeqlD n° 1835 LM-3371.2 From 1872724 to 1873620 Unknown, similar to protein-tyrosine phosphatase
SeqlD n° 1836 LM-3373.2 From 1873764 to 1875116 Unknown, similar to signal récognition particle protein
Ffh
SeqlD n° 1837 LM-3374.2 From 1875129 to 1875461 Unknown, similar to unknown proteins
SeqlD n° 1838 LM-3375.2 From 1358834 to 1359103 ribosomal protein S15
SeqlD n° 1839 LM-3376.1 From 1359352 to 1361523 polynucléotide phosphorylase (PNPase)
SeqlD n° 1840 LM-3377.1 From 1361564 to 1362604 unknown, similar to conserved hypothetical proteins
SeqlD n° 1841 LM-3378.2 From 1362765 to 1363244 unknown, similar to B. subtilis YqzC protein 132
SeqlD n° 1842 LM-3379.2 From 1363272 to 1363628 unknown, similar to B. subtilis YqzD protein
SeqlD n° 1843 LM-338.1 From 117841 to 118956 Unknown, similar to lipase
SeqlD n° 1844 LM-3380.2 From 1918681 to 1919505 unknown, similar to unknown proteins
SeqlD n° 1845 LM-3381.1 From 1919525 to 1920436 unknown, similar to conserved hypothetical proteins
SeqlD n° 1846 LM-3382.1 From 1920436 to 1920900 unknown, highiy similar to signal peptidase II
SeqlD n° 1847 LM-3384.1 From 1920982 to 1922265 unknown, similar to conserved hypothetical proteins
SeqlD n° 1848 LM-3385.1 From 1922439 to 1923809 unknown, similar to conserved hypothetical proteins
SeqlD n° 1849 LM-3387.1 From 1923825 to 1924757 unknown, similar to adhésion binding proteins and lipoproteins with multiple specificity for métal cations
SeqlD n° 1850 LM-3389.1 From 1924754 to 1925596 unknown, similar to intégral membrane proteins, ABC transporter
SeqlD n° 1851 LM-3390.3 From 1925600 to 1926322 unknown, similar to probable ABC transporter,
ATP-binding proteins
SeqlD n° 1852 LM-3391.3 From 262657 to 263262 RNA polymérase sigma-30 factor (sigma-H)
SeqlD n° 1853 LM-3392.1 From 262064 to 262576 Unknown, similar to B. subtilis Yacp protein
SeqlD n° 1854 LM-3393.1 From 261306 to 262061 Unknown, similar to conserved hypothetical proteins like to B. subtilis
YacO protein
SeqlD n° 1855 LM-3394.1 From 260896 to 261306 Unknown, highiy similar to
B. subtilis YazC protein
SeqlD n° 1856 LM-3398.1 From 259477 to 260892 cysteinyl-tRNA synthetase
SeqlD n° 1857 LM-340.1 From 116972 to 117805 Unknwon, similar to transcriptional regulatory proteins, AraC family
SeqlD n° 1858 LM-3400.1 From 258856 to 259470 unknown, similar to serine
O-acetyltransferase
SeqlD n° 1859 LM-3403.3 From 256983 to 258458 unknown, highiy similar to glutamyl-tRNA synthetase
SeqlD n° 1860 LM-3404.3 From 256491 to 256964 Unknown, similar to B. subtilis YacN protein
SeqlD n° 1861 LM-3405.2 From 1862459 to 1862893 Unknown, similar to transcription regulator
SeqlD n° 1862 LM-3406.2 From 1862893 to 1863480 Unknown, weakiy similar to
Nad(P)h Oxidoreductase chain B 133
SeqlD n° ' I863 LM-3407.1 From 1863501 to 1864217 Unknown, similar to unknown proteins
SeqlD n° ' I864 LM-3408.1 From 1864248 to 1864637 Unknown
SeqlD n° ' I865 LM-3409.1 From 1864657 to 1865394 Unknown, similar to E. coli tRNA (guanine-N1) methyltransferase
SeqlD n° ' I866 LM-3410.1 From 1865394 to 1865912 Unknown, similar to putative 16S rRNA processing protein RimM
SeqlD n° ' 1867 LM-3411.1 From 1865922 to 1866308 Unknown, similar to unknown proteins
SeqlD n° ' I868 LM-3412.1 From 1866336 to 1867070 Unknown, similar to unknown proteins
SeqlD n° * 1869 LM-3414.2 From 1867427 to 1867699 ribosomal protein S16
SeqlD n° 1870 LM-3415.4 From 1723649 to 1724122 unknown, some similarity to hypothetical proteins
SeqlD n° ' 1871 LM-3416.3 From 1723356 to 1723598 unknown, some similarity to hypothetical proteins
SeqlD n° ' I872 LM-3417.3 From 1722431 to 1723339 unknown, similar to L- lactate dehydrogenases
SeqlD n° ' 1873 LM-342.1 From 115087 to 116829 Unknown, ABC transporter, ATP-binding protein
SeqlD n° * I874 LM-3420.4 From 1716858 to 1717160 unknown
SeqlD n° * I875 LM-3421.2 From 1748792 to 1749733 unknown; similar to glycerate dehydrogenases
SeqlD n° ' 1876 LM-3422.1 From 1749878 to 1751176 glutamate- 1-semialdehyde aminotransferase
SeqlD n° ' 1877 LM-3424.1 From 1751283 to 1752371 unknown, similar to hypothetical proteins
SeqlD n° 1878 LM-3426.1 From 1752413 to 1752940 unknown, similar to hypothetical proteins
SeqlD n° 1879 LM-3427.1 From 1753095 to 1753841 unknown, similar to glucose 1 -dehydrogenase
SeqlD n° ' 1880 LM-3428.1 From 1753845 to 1754942 unknown, similar to A/G- specific adenine glycosylase
SeqlD n° ' 1881 LM-3429.1 From 1755143 to 1756123 unknown, similar to hypothetical proteins
SeqlD n° * 1882 LM-343.1 From 113313 to 115094 Unknwon, similar to ABC transporter, ATP-binding protein
SeqlD n° ' 1883 LM-3430.2 From 1756156 to 1756617 unknown, similar to deoxyuridine triphosphate nucleotidohydrolases
SeqlD n° " 1884 LM-3431.2 From 2891269 to 2891652 Unknown, hypothetical secreted protein
SeqlD n° 1885 LM-3432.2 From 2890779 to 2891171 Unknown, hypothetical secreted protein
SeqlD n° " 1886 LM-3433.1 From 2890274 to 2890666 Unknown, hypothetical secreted protein
SeqlD n° ' 1887 LM-3434.1 From 2888994 to 2890277 Unknown
SeqlD n° " 1888 LM-3436.1 From 2888639 to 2889001 Unknown 134
SeqlD n° 1889 LM-3439.2 From 2887499 to 2888215 GidB protein SeqlD n° 1890 LM-3442.2 From 885440 to 886882 unknown, similar to Glutamine binding and transport protein
SeqlD n° 1891 LM-3444.1 From 886875 to 887603 unknown, similar to amino acid ABC transporter, ATP- binding protein
SeqlD n° 1892 LM-3445.2 From 887652 to 889502 Unknown, similar to amidases
SeqlD n° 1893 LM-3447.3 From 2068977 to 2069648 Unknown, similar to deoxyribose-phosphate aldolase
SeqlD n° 1894 LM-3448.1 From 2069710 to 2070657 Unknown, similar to transcription repressor of dra/nupC/pdp operon
DeoR
SeqlD n° 1895 LM-3449.1 From 2070754 to 2071170 Unknown, similar to PTS mannose-specific enzyme
IIA component
SeqlD n° 1896 LM-345.1 From 112386 to 113288 Unknown, similar to transcription regulator
SeqlD n° 1897 LM-3450.1 From 2071187 to 2072182 Unknown, similar to opine catabolism protein
SeqlD n° 1898 LM-3451.1 From 2072205 to 2073269 Unknown, weakiy similar to glucosamine-fructose-6- phosphate
I aminotransferase
SeqlD n° 1899 LM-3452.1 From 2073282 to 2074103 Unknown, similar to mannose-specific enzyme
IID component
SeqlD n° 1900 LM-3453.1 From 2074084 to 2074902 Unknown, similar to PTS mannose-specific enzyme
IIC component
SeqlD n° 1901 LM-3455.1 From 2074925 to 2075392 Unknown, similar to PTS mannose-specific enzyme
IIB component
SeqlD n° 1902 LM-3456.2 From 2075412 to 2076113 Unknown, similar to transcription regulator
GntR family
SeqlD n° 1903 LM-3458.2 From 2076115 to 2076846 Unknown, similar to transcription regulator
GntR family
SeqlD n° 1904 LM-3459.2 From 607372 to 608199 Unknown, similar histidinol phosphate phosphatase
SeqlD n° 1905 LM-3460.1 From 608184 to 608480 Unknown, similar to methyltransferase
SeqlD n° 1906 LM-3461. From 608557 to 609588 Unknown
SeqlD n° 1907 LM-3462.2 From 609629 to 610924 Unknown, conserved hypothetical protein
SeqlD n° 1908 LM-3463.2 From 1776257 to 1776829 unknown
SeqlD n° 1909 LM-3465.1 From 1774991 to 1775983 unknown, similar to cell- shape determining proteins 1/77335
135
SeqlD n° 1910 LM-3466.1 From 1773514 to 1774773 unknown, similar to multidrug résistance protein, intégral membrane protein
SeqlD n° 1911 LM-3468.3 From 1772178 to 1773410 unknown, highiy similar to aminopeptidases
SeqlD n° 1912 LM-3469.2 From 833117 to 833587 Unknown SeqlD n° 1913 LM-3473.1 From 831025 to 833073 Unknown, similar to putative Na+/H+ antiporter
SeqlD n° 1914 LM-3475.1 From 830259 to 830897 Unknown, weakiy similar to
GTP-pyrophosphokinase
SeqlD n° 1915 LM-3479.2 From 827666 to 828016 Unknown, similar to B. subtilis YqkB protein
SeqlD n° 1916 LM-3480.3 From 826752 to 827513 Unknown SeqlD n° 1917 LM-3481.2 From 2011128 to 2012438 Unknown, similar to unknown protein
SeqlD n° 1918 LM-3482.1 From 2010089 to 2011105 Unknown, similar to
NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
SeqlD n° 1919 LM-3483.2 From 2009006 to 2009986 Unknown, similar to protein-tyrosine/serine phosphatase
SeqlD n° 1920 LM-3484.1 From 2008319 to 2008594 Unknown, similar to non- specific DNA-binding protein HU
SeqlD n° 1921 LM-3485.1 From 2007515 to 2008084 Unknown, similar to GTP cyclohydrolase I
SeqlD n° 1922 LM-3486.1 From 2006689 to 2007456 Unknown, similar to heptaprenyl diphosphate synthase component I
SeqlD n° 1923 LM-3487.1 From 2005953 to 2006666 Unknown, similar to 2- heptaprenyl-1,4- naphthoquinone methyltransferase
SeqlD n° 1924 LM-3488.1 From 2004977 to 2005942 Unknown, similar to heptaprenyl diphosphate synthase component II
(menaquinone biosynthesis)
SeqlD n° 1925 LM-3489.2 From 2004515 to 2004958 Unknown, similar to nucleoside diphosphate kinase
SeqlD n° 1926 LM-349.1 From 110013 to 112283 Unknown, highiy similar to chitinase B
SeqlD n° 1927 LM-3490.2 From 1029208 to 1029819 unknown, similar to hypothetical protein
SeqlD n° 1928 LM-3491.1 From 1029965 to 1030756 unknown
SeqlD n° 1929 LM-3493.3 From 1030757 to 1032229 unknown, similar to
PHYTOENE
DEHYDROGENASE (EC
1.3.-.-) (PHYTOENE DESATURASE)
SeqlD n° 930 LM-3494.3 From 1032333 to 1032617 unknown, similar to B. subtilis protein YkvS SeqlD n° 931 LM-3495.3 From 1032924 to 1033190 PHOSPHOCARRIER
PROTEIN HPR
(HISTIDINE-CONTAINING
PROTEIN).
SeqlD n° 932 LM-3497.4 From 1033190 to 1034908 phosphotransferase system enzyme I SeqlD n° 933 LM-3498.1 From 1035021 to 1036055 unknown, conserved hypothetical protein SeqlD n° 934 LM-35.1 From 2735279 to 2735953 Unknown, similar to ribulose-5-phosphate 3- epimerase
SeqlD n° 935 LM-3500.1 From 1036068 to 1036928 unknown, similar to 3- hydroxyisobutyrate dehydrogenase (B. subtilis
YkwC protein) SeqlD n° 936 LM-3501.2 From 1036971 to 1038116 unknown, similar to aminotransferases (to B. subtilis PatA protein)
SeqlD n° 937 LM-3504.2 From 641208 to 642308 UNknown, similar to cell surface protein
SeqlD n° 938 LM-3505.1 From 642414 to 642914 Unknown, weakiy similar to transcription regulator
SeqlD n° 939 LM-3506.1 From 642962 to 643348 Unknown SeqlD n° 940 LM-3507.1 From 643400 to 643744 Unknown, similar to B. subtilis YvIA protein
SeqlD n° 941 LM-3508.2 From 643891 to 645231 Unknown, conserved hypothetical membrane protein
SeqlD n° 942 LM-351.2 From 109349 to 109720 Unknwon SeqlD n° 943 LM-3510.2 From 2145652 to 2146044 Unknown SeqlD n° 944 LM-3511.1 From 2146089 to 2146298 Unknown SeqlD n° 945 LM-3512.1 From 2146449 to 2147426 Unknown, similar to conjugated bile acid hydrolase
SeqlD n° 946 LM-3514.2 From 2147684 to 2149312 class I heat-shock protein
(chaperonin) GroEL SeqlD n° 947 LM-3517.2 From 1888615 to 1890273 Unknown, similar to unknown proteins SeqlD n° 948 LM-3518.1 From 1887913 to 1888575 Unknown, similar to phosphoglycerate dehydrogenase
SeqlD n° 949 LM-3519.1 From 1886887 to 1887777 Unknown, similar to L- serine dehydratase SeqlD n° 950 LM-352.2 From 108624 to 109256 Unknwon, similar to NADH oxidase SeqlD n° 951 LM-3520.2 From 1884846 to 1886894 Unknown, similar to ATP- dependent DNA helicase recG
SeqlD n° 1952 LM-3523.1 From 2143109 to 2144734 Unknown, similar to copper export proteins
SeqlD n° 1953 LM-3524.1 From 2142480 to 2143097 Unknown, similar to unknown protein
SeqlD n° 1954 LM-3525.1 From 2141820 to 2142461 Unknown, similar to unknown protein
SeqlD n° 1955 LM-3527.1 From 2141071 to 2141814 Unknown, similar to potassium channel subunit
SeqlD n° 1956 LM-3528.2 From 2140046 to 2140963 Unknown, similar to heme
O oxygenase
SeqlD n° 1957 LM-353.1 From 108320 to 108610 Unknwon
SeqlD n° 1958 LM-3533.1 From 1053426 to 1054277 unknown
SeqlD n° 1959 LM-3534.1 From 1054319 to 1055284 unknown, similar to B. subtilis LytR protein
SeqlD n° 1960 LM-3535.1 From 1055393 to 1057060 unknown, similar to conserved hypothetical proteins (in particular B. subtilis YkqC)
SeqlD n° 1961 LM-3537.1 From 1057761 to 1058531 unknown, similar to conserved hypothetical proteins
SeqlD n° 1962 LM-3538.3 From 1058580 to 1059608 unknown, similar to transcriptional regulator,
Lacl family
SeqlD n° 1963 LM-3539.1 From 2286839 to 2287183 Unknown
SeqlD n° 1964 LM-354.1 From 107915 to 108208 Unknwon, similar to transcription regulator
SeqlD n° 1965 LM-3541.1 From 2287518 to 2288513 tryptophanyl-tRNA synthetase
SeqlD n° 1966 LM-3543.1 From 2288571 to 2288987 Unknown, similar to unknown protein
SeqlD n° 1967 LM-3544.1 From 2288971 to 2289423 Unknown, similar to transcription regulator
SeqlD n° 1968 LM-3547.1 From 2289545 to 2290786 Unknown, similar to 3- oxoacyl-acyl-carrier protein synthase
SeqlD n° 1969 LM-3549.2 From 2290989 to 2291927 Unknown, similar to 3- oxoacyl- acyl-carrier protein synthase
SeqlD n° 1970 LM-355.1 From 107499 to 107849 Unknwon
SeqlD n° 1971 LM-3551.1 From 1242100 to 1244508 phenylalanyl-tRNA synthetase beta subunit
SeqlD n° 1972 LM-3553.2 From 1241048 to 1242100 phenylalany-tRNA synthetase beta subunit
SeqlD n° 1973 LM-3555.2 From 2305632 to 2305994 Unknown, similar to unknown protein
SeqlD n° 1974 LM-3556.1 From 2305180 to 2305602 Unknown, similar to histidine triad (HIT) protein SeqlD n° 975 LM-3557.1 From 2304267 to 2305019 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 976 LM-3558.1 From 2303060 to 2304283 Unknown, similar to ABC transporter (membrane protein)
SeqlD n° 977 LM-3559.1 From 2302518 to 2303021 Unknown, similar to unknown protein
SeqlD n° 978 LM-356.1 From 106988 to 107377 unknown SeqlD n° 979 LM-3560.1 From 2301260 to 2302321 Unknown, similar to uroporphyrinogen III decarboxylase
SeqlD n° 980 LM-3561.1 From 2300334 to 2301263 Unknown, similar to ferrochelatase
SeqlD n° 981 LM-3562.2 From 2299833 to 2300177 Unknown SeqlD n° 982 LM-3563.2 From 1548034 to 1548456 Unknown, similar to unknown protein
SeqlD n° 983 LM-3565.2 From 1548701 to 1549906 Unknown, similar to ammonium transporter
NrgA
SeqlD n° 984 LM-3566.1 From 1549920 to 1550285 Unknown, similar to nitrogen regulatory PII protein
SeqlD n° 985 LM-3567.1 From 1550435 to 1550815 Unknown SeqlD n° 986 LM-3569.1 From 1550854 to 1552629 aspartyl-tRNA synthetase SeqlD n° 987 LM-357.1 From 105951 to 106862 Unknown, similar to PTS system mannose-specific, factor IID
SeqlD n° 988 LM-3570.2 From 1552632 to 1553909 histidyl-tRNA synthetase SeqlD n° 989 LM-3571.4 From 1713234 to 1715099 unknown, similar to asparagine synthetase
SeqlD n° 990 LM-3572.1 From 1712634 to 1713209 unknown, similar to conserved hypothetical protein
SeqlD n° 991 LM-3573.2 From 1711672 to 1712637 unknown, similar to conserved hypothetical proteins
SeqlD n° 992 LM-3574.3 From 896003 to 897028 unknown, similar to transcription regulator lacl family
SeqlD n° 993 LM-3576.1 From 895278 to 895991 unknown, similar to carboxylesterase SeqlD n° 994 LM-3577.1 From 893838 to 895211 UDP-N- acetylmuramoylalanyl-D- glutamyl-2,6- diamînopimelate-D-alanyl-
D-alanyl ligase
SeqlD n° 995 LM-3578.1 From 892662 to 893774 D-alanine-D-alanine ligase SeqlD n° 996 LM-3579.1 From 892192 to 892512 Unknown, similar to E. coli
SugE protein
(transmembrane chaperone) SeqlD n° 1997 LM-358.1 From 105123 to 105929 Unknown, similar to PTS system mannose-specific, factor IIC
SeqlD n° 1998 LM-3581.1 From 891848 to 892189 Unknown, similar to E. coli
SugE protein
(transmembrane chaperone)
SeqlD n° 1999 LM-3582.1 From 891277 to 891831 Unknown, similar to transcription regulator
TetR/AcrR family
SeqlD n° 2000 LM-3583.3 From 890439 to 891197 Unknown SeqlD n° 2001 LM-3585.2 From 1926935 to 1928425 unknown, similar to carboxy-terminal processing proteinase
SeqlD n° 2002 LM-3588.1 From 1928798 to 1931011 unknown, similar to heavy metal-transporting
ATPases
SeqlD n° 2003 LM-3589.1 From 1931026 to 1931319 unknown, similar to conserved hypothetical proteins
SeqlD n° 2004 LM-359.1 From 104134 to 105099 Unknown, similar to PTS system mannose-specific, factor IIAB
SeqlD n° 2005 LM-3590.2 From 1931442 to 1932266 unknown, similar to similar to D-alanyl-D-alanine carboxypeptidases
SeqlD n° 2006 LM-3591.3 From 1932324 to 1933025 purine nucleoside phosphorylase
SeqlD n° 2007 LM-3592.2 From 2487261 to 2488337 Unknown
SeqlD n° 2008 LM-3593.1 From 2488379 to 2489209 Unknown, conserved lipoprotein
SeqlD n° 2009 LM-3596.1 From 2489274 to 2489948 Unknown, similar to ABC transporter, permease protein
SeqlD n° 2010 LM-3597.1 From 2489945 to 2490967 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 2011 LM-3599.1 From 2491552 to 2492694 Unknown, similar to two- component sensor histidine kinase
SeqlD n° 2012 LM-36.1 From 2735962 to 2736402 Unknwon, similar to ribose
5-phosphate epimerase
SeqlD n° 2013 LM-360.1 From 103204 to 103836 unknown SeqlD n° 2014 LM-3600.1 From 2492684 to 2493379 Unknown, similar to two- component response regulator
SeqlD n° 2015 LM-3601.3 From 2493454 to 2494329 Unknown, conserved hypothetical protein
SeqlD n° 2016 LM-3602.2 From 1435635 to 1437866 pyruvate formate-lyase SeqlD n° 2017 LM-3603.1 From 1437943 to 1438689 pyruvate-formate lyase activating enzyme SeqlD n° 2018 LM-3604.1 From 1438724 to 1439254 Unknown, similar to unknown proteins SeqlD n° 2019 LM-3605.2 From 1439421 to 1440614 Unknown, similar to multidrug-efflux transporter SeqlD n° 2020 LM-3608.2 From 1104863 to 1105864 unknown, similar to TEICHOIC ACID TRANSLOCATION ATP- BINDING PROTEIN TAGH (ABC transporter)
SeqlD n° 2021 LM-361.1 From 102468 to 103028 unknown SeqlD n° 2022 LM-3612.1 From 1107975 to 1109663 unknown, similar to
TEICHOIC ACID
BIOSYNTHESIS PROTEIN
B PRECURSOR
SeqlD n° 2023 LM-3613.1 From 1109705 to 1110577 unknown, similar to putative UDP-glucose pyrophosphorylases
SeqlD n° 2024 LM-3614.3 From 1110769 to 1113627 unknown, similar to B. subtilis YfhO protein
SeqlD n° 2025 LM-3615.2 From 1277312 to 1278025 Unknown, similar to transcription regulator
GntR family
SeqlD n° 2026 LM-3616.1 From 1278056 to 1279702 Unknown, similar to alpha, alpha- phosphotrehalase
SeqlD n° 2027 LM-3617.3 From 1279721 to 1281205 Unknown, similar to PTS system trehalose spécifie enzyme IIBC
SeqlD n° 2028 LM-3618.3 From 1434661 to 1435209 Unknown, similar to putative anti-terminator regulatory protein
SeqlD n° 2029 LM-362.1 From 102155 to 102478 Unknwon, similar to ATP synthase epsilon chain
SeqlD n° 2030 LM-3620.2 From 1432839 to 1434644 DNA mismatch repair protein
SeqlD n° 2031 LM-3623.1 From 1430237 to 1432819 DNA mismatch repair
(récognition)
SeqlD n° 2032 LM-3624.2 From 1429765 to 1430127 Unknown, similar to B. subtilis YmcA protein
SeqlD n" 2033 LM-3625.2 From 758656 to 759396 Unknown, similar to riboflavin kinase / FAD synthase
SeqlD n° 2034 LM-3626.2 From 756738 to 758543 Unknown, similar to L- glutamine-D-fructose-6- phosphate amidotransferase
SeqlD n° 2035 LM-3627.2 From 598020 to 598925 • Unknown, putative membrane protein
SeqlD n° 2036 LM-3628.2 From 598968 to 600344 Unknown, similar to NADP- specific glutamate dehydrogenase 141
SeqlD n° 2037 LM-363.1 From 100772 to 102142 Unknown, similar to ATP synthase beta chain
SeqlD n° 2038 LM-3630.4 From 134782 to 136290 Unkown, similar to inosine monophosphate dehydrogenase
SeqlD n° 2039 LM-3631.2 From 133961 to 134710 Unknwon, conserved hypothetical protein
SeqlD n° 2040 LM-3632.2 From 2292047 to 2293174 Unknown, similar to N- acetylmuramoyl-L-alanine amidase and to internalin B
SeqlD n° 2041 LM-3633.1 From 2293775 to 2294464 Unknown, similar to phosphoglyceromutase 1
SeqlD n° 2042 LM-3635.2 From 2294555 to 2297155 Unknown, similar to endopeptidase Clp ATP- binding chain B (ClpB)
SeqlD n° 2043 LM-3636.2 From 1281321 to 1281773 unknown SeqlD n° 2044 LM-3637.1 From 1281820 to 1282284 unknown SeqlD n° 2045 LM-3638.1 From 1282473 to 1283393 unknown SeqlD n° 2046 LM-3639.1 From 1283413 to 1284660 gamma-glutamyl phosphate reductase
SeqlD n° 2047 LM-364.1 From 99902 to 100771 unknwon, similar to ATP synthase gamma chain
SeqlD n° 2048 LM-3640.1 From 1284644 to 1285474 gamma-glutamyl kinase SeqlD n° 2049 LM-3643.3 From 1285610 to 1286749 unknown SeqlD n° 2050 LM-3644.4 From 1286830 to 1287225 unknown, similar to transcriptional regulator (phage-related)
SeqlD n° 2051 LM-3645.2 From 1765915 to 1767294 unknown, similar to similar to RNA methyltransferases SeqlD n° 2052 LM-3647.1 From 1765499 to 1765900 unknown, similar to glutatnione transferase - fosfomycin résistance protein
SeqlD n° 2053 LM-3648.1 From 1765117 to 1765470 unknown SeqlD n° 2054 LM-3649.1 From 1763941 to 1764843 unknown, some similarities to methyl-accepting chemotaxis proteins
SeqlD n° 2055 LM-3650.1 From 1763209 to 1763751 unknown, similar to ribosomal-protein-alanine N-acetyltransferase
SeqlD n° 2056 LM-3652.2 From 1762200 to 1763165 unknown, similar to putative transmembrane proteins
SeqlD n° 2057 LM-3656.2 From 2840286 to 2841896 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 2058 LM-3657.2 From 2839698 to 2840228 Unknown, similar to unknown protein
SeqlD n° 2059 LM-3658.1 From 1595227 to 1597149 threonyl-tRNA synthetase SeqlD n° 2060 LM-3659.1 From 1597499 to 1598422 primosome component
(helicase loader) Dnal SeqlD n° 2061 LM-366.1 From 98409 to 99905 Unknwon, similar to ATP synthase alpha chain SeqlD n° 2062 LM-3660.2 From 1598432 to 1599808 chromosome réplication initiation / membrane attachment protein DnaB
SeqlD n° 2063 LM-3662.3 From 2708276 to 2709358 Unknown, conserved hypothetical lipoprotein
SeqlD n° 2064 LM-3663.2 From 2707235 to 2708194 Unknown, weakiy similar to
E. coli MenA protein
SeqlD n° 2065 LM-3664.1 From 2706415 to 2707215 Unknown, similar to B. subtilis YbaF protein
SeqlD n° 2066 LM-3666.1 From 2705746 to 2706054 ribosomal protein S10 SeqlD n° 2067 LM-3667.1 From 2705082 to 2705711 ribosomal protein L3 SeqlD n° 2068 LM-3668.1 From 2704433 to 2705056 ribosomal protein L4 SeqlD n° 2069 LM-3669.1 From 2704149 to 2704433 ribosomal protein L23 SeqlD n° 2070 LM-367.1 From 97381 to 98412 Unknwon, weakiy similar to ATP synthase delta chain
SeqlD n° 2071 LM-3671.2 From 2703275 to 2704108 ribosomal protein L2 SeqlD n° 2072 LM-3673.2 From 2033587 to 2034528 Unknown, similar to ferrichrome binding protein
SeqlD n° 2073 LM-3674.1 From 2032460 to 2033485 Unknown, similar to ferrichrome ABC transporter (permease)
SeqlD n° 2074 LM-3676.2 From 2031438 to 2032460 Unknown, similar to ferrichrome ABC transporter (permease)
SeqlD n° 2075 LM-3677.2 From 1756864 to 1757673 unknown, similar to hypothetical proteins
SeqlD n° 2076 LM-3678.1 From 1757771 to 1758673 unknown, similar to CDP- abequose synthase
SeqlD n° 2077 LM-3679.2 From 1758694 to 1761291 unknown, similar to putative membrane proteins
SeqlD n° 2078 LM-368.1 From 97127 to 97369 Unknwon, similar to ATP synthase C chain
SeqlD n° 2079 LM-3680.2 From 1761318 to 1761992 unknown, similar to unknown proteins
SeqlD n° 2080 LM-3681.2 From 1708312 to 1708518 unknown SeqlD n° 2081 LM-3684.3 From 1708857 to 1711268 leucyl-tRNA synthetase SeqlD n° 2082 LM-3692.1 From 2161161 to 2162054 Unknown SeqlD n° 2083 LM-3693.1 From 2160538 to 2161164 unknown SeqlD n° 2084 LM-3694.1 From 2160043 to 2160432 Unknown, similar to unknown proetin
SeqlD n° 2085 LM-3695.2 From 980177 to 981307 Unknown, similar to B. subtilis ComEC protein
SeqlD n° 2086 LM-3696.1 From 981743 to 982960 unknown, hypothetical transport protein
SeqlD n° 2087 LM-3697.2 From 982957 to 983646 unknown, similar to transcription regulator
SeqlD n° 2088 LM-3698.2 From 983768 to 984814 unknown, conserved hypothetical membrane protein SeqlD n° 2089 LM-3699.2 From 2658024 to 2658851 Unknown, conserved hypothetical protein
SeqlD n° 2090 LM-370.1 From 94764 to 97070 unknwon
SeqlD n° 2091 LM-3701.3 From 1936764 to 1937603 unknown, similar to hypothetical proteins
SeqlD n° 2092 LM-3703.3 From 1935964 to 1936749 unknown, similar to hypothetical proteins
SeqlD n° 2093 LM-3704.3 From 1935334 to 1935948 unknown, similar to hypothetical proteins
SeqlD n° 2094 LM-3705.1 From 1934765 to 1935298 unknown, similar to peptidyl methionine sulfoxide reductases
SeqlD n° 2095 LM-3706.1 From 1934321 to 1934758 unknown, similar to transcriptional regulator
(PilB family)
SeqlD n° 2096 LM-3707.3 From 1933333 to 1934238 unknown, similar to dehydogenases and hypothetical proteins
SeqlD n° 2097 LM-3708.2 From 822611 to 823531 Unknown, conserved hypothetical protein
SeqlD n° 2098 LM-3709.1 From 821886 to 822527 Unknown, similar to B. subtilis YwnB protein
SeqlD n° 2099 LM-3710.1 From 821070 to 821771 Unknown, similar to conserved hypothetical protein
SeqlD n° 2100 LM-3711.3 From 820145 to 821035 Unknown, similar to conserved hypothetical protein
SeqlD n° 2101 LM-3712.2 From 1767359 to 1767739 unknown, similar to conserved hypothetical proteins
SeqlD n° 2102 LM-3713.1 From 1767846 to 1768490 unknown, similar to deoxyguanosine kinase/deoxyadenosine kinase(l) subunit
SeqlD n° 2103 LM-3714.1 From 1768520 to 1769389 unknown, similar to transport proteins
SeqlD n° 2104 LM-3715.1 From 1769720 to 1770526 unknown, similar to aminoglycoside N3 - acetyltransferases
SeqlD n° 2105 LM-3716.2 From 1770648 to 1771406 unknown, similar to methionine aminopeptidases
SeqlD n° 2106 LM-3717.2 From 1818007 to 1818516 Unknown SeqlD n° 2107 LM-3718.1 From 1817112 to 1817879 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 2108 LM-3719.3 From 1815143 to 1817122 Unknown, similar to ABC transporter (permease) SeqlD n° 2109 LM-3720.2 From 1293088 to 1293654 unknown, similar to type-l signal peptidase SeqlD n° 2110 LM-3722.1 From 1291710 to 1292969 ATP-dependent Clp protease ATP-binding subunit ClpX
SeqlD n° 2111 LM-3724.1 From 1290241 to 1291524 trigger factor (prolyl isomerase)
SeqlD n° 2112 LM-3725.2 From 1289188 to 1290126 unknown SeqlD n° 2113 LM-3726.3 From 1703330 to 1703824 unknown, putative cellsurface protein
SeqlD n° 2114 LM-3727.1 From 1704618 to 1705181 unknown, similar to unknown proteins
SeqlD n° 2115 LM-3728.1 From 1705199 to 1705630 unknown SeqlD n° 2116 LM-373.1 From 88888 to 94767 Unknown SeqlD n° 2117 LM-3732.2 From 1706204 to 1707088 similar to translation elongation factor
SeqlD n° 2118 LM-3733.3 From 1707168 to 1707917 30S ribosomal protein S2 SeqlD n° 2119 LM-3735.3 From 1645412 to 1645936 Unknown, similar to gênerai stress protein
SeqlD n° 2120 LM-3736.3 From 1644106 to 1645191 3-deoxy-D-arabino- heptulosonate 7-phosphate synthase
SeqlD n° 2121 LM-3737.1 From 1642865 to 1643872 catabolite control protein A SeqlD n° 2122 LM-3739.2 From 1641284 to 1642543 tyrosyl-tRNA synthetase SeqlD n° 2123 LM-374.1 From 88121 to 88810 unknwon SeqlD n° 2124 LM-3746.2 From 1428442 to 1428939 Unknown, similar to N- acetyltransferase
SeqlD n° 2125 LM-3747.1 From 1426766 to 1428328 Unknown, similar to unknown protein
SeqlD n° 2126 LM-3749.2 From 1425419 to 1426465 Recombination protein recA
SeqlD n° 2127 LM-375.1 From 86747 to 87730 Unknwon, similar to oxidoreductases
SeqlD n° 2128 LM-3750.2 From 356890 to 357705 Unknown, similar to transposase
SeqlD n° 2129 LM-3754.2 From 360172 to 360507 Unknown SeqlD n° 2130 LM-3756.2 From 1407859 to 1408818 Unknown, similar to unknown protein
SeqlD n° 2131 LM-3757.2 From 1406742 to 1407818 Unknown, similar to unknown protein
SeqlD n° 2132 LM-3758.2 From 824936 to 826396 Unknown, similar to lysine- specific permease
SeqlD n° 2133 LM-3759.2 From 824423 to 824884 Unknown SeqlD n° 2134 LM-376.1 From 86323 to 86691 Unknown, similar to transcription regulator
(merR family)
SeqlD n° 2135 LM-3763.1 From 1659457 to 1660926 Unknown, similar to multidrug-efflux transporter
SeqlD n° 2136 LM-3764.1 From 1660923 to 1661363 Unknown, similar to transcription regulator
MarR family
SeqlD n° 2137 LM-3766.3 From 1661588 to 1662457 D-Amino Acid
Aminotransferase
SeqlD n° 2138 LM-3767.2 From 2212384 to 2212620 unknown SeqlD n° 2139 LM-3768.2 From 2213218 to 2215041 Unknown, similar to unknown protein
SeqlD n° 2140 LM-3769.2 From 2306177 to 2306716 Unknown SeqlD n° 2141 LM-377.1 From 85902 to 86228 Unknwon SeqlD n° 2142 LM-3770.2 From 2306833 to 2307714 Unknown, similar to post- translocation molecular chaperone
SeqlD n° 2143 LM-3772.2 From 2307755 to 2308696 Unknown, similar to S. aureus Cbf1 protein
SeqlD n° 2144 LM-3774.3 From 991810 to 992865 unknown, similar to
UNDECAPRENYL-
PHOSPHATE N-
ACETYLGLUCOSAMINYL
TRANSFERASE
SeqlD n° 2145 LM-3775.1 From 990968 to 991690 unknown, similar to transcription regulator
(GntR family)
SeqlD n° 2146 LM-3776.2 From 990248 to 990952 GLUCOSAMINE-6-
PHOSPHATE
ISOMERASE (EC 5.3.1.10)
(GLUCOSAMINE-6-
PHOSPHATE
DEAMINASE) (GNPDA)
(GLCN6P DEAMINASE).
SeqlD n° 2147 LM-3778.2 From 1861907 to 1862251 ribosomal protein L19
SeqlD n° 2148 LM-3779.3 From 1860200 to 1861090 internalin C
SeqlD n° 2149 LM-378.1 From 85025 to 85627 Unknwon
SeqlD n° 2150 LM-3783.2 From 989099 to 990232 N-
ACETYLGLUCOSAMINE-
6-PHOSPHATE
DEACETYLASE (EC
3.5.1.25) (GLCNAC 6-P
DEACETYLASE).
SeqlD n° 2151 LM-3785.2 From 988065 to 988910 unknown
SeqlD n° 2152 LM-379.1 From 84451 to 84849 Unknwon
SeqlD n° 2153 LM-3790.3 From 1420076 to 1421362 Unknown, similar to putative protease
SeqlD n° 2154 LM-3791.2 From 1778415 to 1779482 unknown, similar to hypothetical proteins
SeqlD n° 2155 LM-3792.1 From 1777763 to 1778338 unknown, similar to putative transcription regulators
SeqlD n° 2156 LM-3793.2 From 1776910 to 1777578 unknown, similar to hypothetical proteins
SeqlD n° 2157 LM-3795.3 From 1421454 to 1422185 Unknown, similar to 3- ketoacyl-acyl carrier protein reductase
SeqlD n° 2158 LM-3797.2 From 1422236 to 1423165 Unknown, similar to unknown protein
SeqlD n° 2159 LM-3798.1 From 1423255 to 1423833 Unknown, similar to phosphatidylglycerophosph ate synthase
SeqlD n° 2160 LM-380.1 From 82959 to 84437 Unknwon SeqlD n° 2161 LM-3800.3 From 1423902 to 1425146 Unknown, similar to competence-damage inducible protein CinA
SeqlD n° 2162 LM-3801.3 From 984823 to 985722 unknown SeqlD n° 2163 LM-3802.4 From 985743 to 986564 unknown SeqlD n° 2164 LM-3806.3 From 1578625 to 1579425 unknown, highiy similar to cell division inhibitor
(septum placement) protein
MinD
SeqlD n° 2165 LM-3807.3 From 1577212 to 1578573 Unknown, similar to ribonuclease G
SeqlD n° 2166 LM-3808.2 From 2298927 to 2299712 Unknown SeqlD n° 2167 LM-3809.2 From 2298093 to 2298863 Unknown, similar to unknown protein
SeqlD n° 2168 LM-3810.3 From 333534 to 335195 Unknown, similar to unknown protein
SeqlD n° 2169 LM-3811.3 From 335350 to 336426 Unknown SeqlD n° 2170 LM-3812.3 From 1662570 to 1663982 Unknown, similar to Xaa-
His dipeptidase
SeqlD n° 2171 LM-3818.1 From 2211280 to 2212245 Unknown, similar to transcription regulator, Lacl family
SeqlD n° 2172 LM-3819.1 From 2210609 to 2211244 Unknown SeqlD n° 2173 LM-382.1 From 81661 to 82617 Unknown, similar to phosphoglycerate dehydrogenase
SeqlD n° 2174 LM-3821.1 From 2159697 to 2160053 Unknown, similar to unknown protein
SeqlD n° 2175 LM-3822.1 From 2149348 to 2149632 class I heat-shock protein
(chaperonin) GroES
SeqlD n° 2176 LM-3823.3 From 2943120 to 2943479 ribonuclease P protein component
SeqlD n° 2177 LM-3825.4 From 2943871 to 2944341 hypothetical protein SeqlD n° 2178 LM-3826.1 From 1240358 to 1240681 unknown, similar to unknown protein
SeqlD n° 2179 LM-383.1 From 80892 to 81530 Unknwon, conserved hypothetical protein
SeqlD n° 2180 LM-3845.2 From 979762 to 980064 unknown, similar to B. subtilis YneR protein
SeqlD n° 2181 LM-3846.1 From 979059 to 979529 non-heme iron-binding ferritin
SeqlD n° 2182 LM-3847.1 From 940316 to 940696 unknown, conserved hypothetical protein
SeqlD n° 2183 LM-3848.1 From 1357753 to 1358697 unknown, highiy similar to riboflavin kinase and FAD synthase
SeqlD n° 2184 LM-385.1 From 79817 to 80869 Unknwon, similar to E. coli
Ada protein (O6- methylguanine-DNA methyltransferase)
SeqlD n° 2185 LM-3851.1 From 1378840 to 1379901 Unknown, similar to aminopeptidase P SeqlD n° 2186 LM-3853.2 From 501693 to 501959 Unknown, weakiy similar to transposase
SeqlD n° 2187 LM-3856.2 From 1953836 to 1954237 unknown, similar to similar to RNase Hl
SeqlD n° 2188 LM-3857.1 From 1963240 to 1963605 unknown, similar to conserved hypothetical proteins
SeqlD n° 2189 LM-3858.2 From 2030744 to 2031196 Unknown, similar to transcriptional regulator
(Fur family)
SeqlD n° 2190 LM-386.1 From 79047 to 79820 Unknwon, similar to carboxyphosphonoenolpyr uvate phosphonomutase
SeqlD n° 2191 LM-3863.2 From 2494891 to 2495268 Unknown, similar to glycine cleavage system protein H
SeqlD n° 2192 LM-3865.2 From 200094 to 200402 Unknwon, similar to B. subtilis SpoVG protein
SeqlD n° 2193 LM-3867.1 From 1890288 to 1890653 Unknown, similar to unknown protein
SeqlD n° 2194 LM-3868.2 From 1428945 to 1429748 Unknown, conserved hypothetical protein
SeqlD n° 2195 LM-3869.2 From 1440748 to 1441296 Unknown SeqlD n° 2196 LM-387.1 From 78362 to 78811 Unknown SeqlD n° 2197 LM-3871.1 From 2100845 to 2101366 Unknown, similar to unknown protein
SeqlD n° 2198 LM-3872.1 From 2100224 to 2100751 Unknown, similar to cell- division initiation protein
(septum placement)
SeqlD n° 2199 LM-3873.1 From 2891649 to 2892032 Unknown, hypothetical secreted protein
SeqlD n° 2200 LM-3876.1 From 520961 to 521536 Unknown SeqlD n° 2201 LM-3878.2 From 640625 to 641215 Unknown SeqlD n° 2202 LM-3879.2 From 640300 to 640632 Unknown, conserved hypothetical protein
SeqlD n° 2203 LM-388.1 From 78090 to 78374 unknown SeqlD n° 2204 LM-3883.1 From 1664057 to 1664470 Unknown, weakiy similar to
E. coli MutT protein (dGTP pyrophosphohydrolase
SeqlD n° 2205 LM-3886.1 From 1702695 to 1703315 unknown, putative cellsurface protein
SeqlD n° 2206 LM-3887.1 From 1038336 to 1038557 unknown
SeqlD n° 2207 LM-3889.1 From 2524168 to 2524911 Unknown, similar to carboxylesterase
SeqlD n° 2208 LM-389.1 From 77149 to 77406 unknown
SeqlD n° 2209 LM-3890.1 From 2523835 to 2524068 Unknown
SeqlD n° 2210 LM-3891.1 From 1724196 to 1724462 unknown, similar to conserved hypothetical proteins
SeqlD n° 2211 LM-3893.1 From 611240 to 612634 Unknown, similar to beta- glucosidase SeqlD n° 2212 LM-3896.1 From 1546608 to 1547885 Unknown, similar to unknown protein SeqlD n° 2213 LM-3897.3 From 1514257 to 1515288 Unknown, similar to unknown protein
SeqlD n° 2214 LM-3898.1 From 1594535 to 1595119 Unknown, similar to hypothetical GTP binding protein
SeqlD n° 2215 LM-39.1 From 2736597 to 2737628 Unknown, similar to polyol dehydrogenase
SeqlD n° 2216 LM-390.3 From 76262 to 76984 unknown
SeqlD n° 2217 LM-3902.4 From 1715236 to 1716435 Unknown, similar to S- methionine adenosyltransferase
SeqlD n° 2218 LM-3904.3 From 819389 to 820039 Unknown SeqlD n° 2219 LM-3905.2 From 502899 to 504629 Unknown SeqlD n° 2220 LM-3907.1 From 1234779 to 1235330 unknown SeqlD n° 2221 LM-3908.1 From 1234465 to 1234776 unknown, similar to unknown protein
SeqlD n° 2222 LM-3910.2 From 1409070 to 1409996 Unknown, similar to unknown protein
SeqlD n° 2223 LM-3912.1 From 2709522 to 2710421 Unknown, conserved lipoprotein
SeqlD n° 2224 LM-3914.1 From 2297304 to 2297981 Unknown, similar to unknown protein
SeqlD n° 2225 LM-3915.1 From 2145127 to 2145513 Unknown, similar to large conductance mechanosensitive channel protein
SeqlD n° 2226 LM-392.3 From 75832 to 76125 unknown SeqlD n° 2227 LM-3922.2 From 987735 to 988049 Unknown SeqlD n° 2228 LM-3924.2 From 986722 to 987180 Unknown SeqlD n° 2229 LM-3929.1 From 833615 to 834076 unknown SeqlD n° 2230 LM-393.3 From 75342 to 75665 Unknwon SeqlD n° 2231 LM-3934.1 From 356567 to 356872 Unknown, similar to transposase
SeqlD n° 2232 LM-3942.3 From 2633930 to 2634850 Unknown, conserved hypothetical protein
SeqlD n° 2233 LM-3943.4 From 1576107 to 1576397 ribosomal protein L27
SeqlD n° 2234 LM-3944.4 From 1576411 to 1576728 Unknown, similar to unknown protein
SeqlD n° 2235 LM-3947.1 From 1599814 to 1600278 Unknown, similar to unknown protein
SeqlD n° 2236 LM-3949.2 From 1638420 to 1638908 Unknown, similar to unknown protein
SeqlD n° 2237 LM-395.2 From 74239 to 75228 Unknown, similar to dinitrogenase reductase
ADP-ribosylation system
SeqlD n° 2238 LM-3950.2 From 1639120 to 1639722 ribosomal protein S4
SeqlD n° 2239 LM-3951.1 From 1640225 to 1641004 Unknown
SeqlD n° 2240 LM-3953.1 From 502424 to 502660 Hypothetical orf
SeqlD n° 2241 LM-3954.2 From 504674 to 504997 Unknown SeqlD n° 2242 LM-3955.1 From 1307763 to 1308170 unknown, conserved hypothetical protein, similar to B. subtilis YneT protein
SeqlD n° 2243 LM-3957.1 From 2360908 to 2361141 Unknown SeqlD n° 2244 LM-3958.1 From 2360435 to 2360713 Unknown, similar to compétence transcription factor ComK, N terminal part
SeqlD n° 2245 LM-3959.2 From 2359948 to 2360184 Unknown
SeqlD n° 2246 LM-396.2 From 1859272 to 1859787 translation initiation factor
IF-3
SeqlD n° 2247 LM-3961.1 From 2494522 to 2494806 Unknown, similar to thioredoxin
SeqlD n° 2248 LM-3966.4 From 1739674 to 1740849 unknown, similar to transmembrane transport proteins
SeqlD n° 2249 LM-397.3 From 1858651 to 1859010 ribosomal protein L20 SeqlD n° 2250 LM-3970.1 From 1933037 to 1933270 unknown, similar to hypoyhetical protein
SeqlD n° 2251 LM-3972.2 From 818789 to 819265 Unknown, similar to transcription regulator
(EbsC from Enterococcus faecalis)
SeqlD n° 2252 LM-3973.2 From 1136900 to 1137400 Unknown, similar to lipoprotein signal peptidase SeqlD n° 2253 LM-3976.4 From 267530 to 267916 Unknown, similar to repressor (penicilinase repressor)
SeqlD n° 2254 LM-3978.3 From 267010 to 267372 ribosomal protein L12 SeqlD n° 2255 LM-3979.3 From 266431 to 266931 ribosomal protein L10 SeqlD n° 2256 LM-398.1 From 1857856 to 1858611 Unknown, similar to 3 -exo- deoxyribonuclease exoA
SeqlD n° 2257 LM-3981.1 From 2361897 to 2362274 protein gp30
[Bacteriophage A118]
SeqlD n° 2258 LM-3982.1 From 2362306 to 2362656 protein gp29
[Bacteriophage A118]
SeqlD n° 2259 LM-3984.1 From 270372 to 270977 Unknown, conserved hypothetical protein
SeqlD n° 2260 LM-399.1 From 1857242 to 1857772 Unknown SeqlD n° 2261 LM-3990.1 From 222983 to 223261 Unknwon, highiy similar to
B. subtilis YabO protein
SeqlD n° 2262 LM-3993.1 From 200522 to 200830 Unknown, similar to B. subtilis SpoVG protein
SeqlD n° 2263 LM-3995.1 From 143016 to 143258 unknown SeqlD n° 2264 LM-3998.1 From 823638 to 824168 Unknown, conserved hypothetical protein
SeqlD n° 2265 LM-3999.3 From 810985 to 811620 Unknown, similar to acyl- carrier protein phosphodiesterase and to
NAD(P)H dehydrogenase
SeqlD n° 2266 LM-4.1 From 2713398 to 2713940 Unknown SeqlD n° 2267 LM-40.1 From 2737630 to 2738682 Unknown, similar to sorbitol dehydrogenase
SeqlD n° 2268 LM-4003.2 From 1513917 to 1514171 ribosomal protein S20
SeqlD n° 2269 LM-401.1 From 1855952 to 1857184 Unknown, similar to aminotripeptidase
(peptidase T)
SeqlD n° 2270 LM-4013.2 From 1926482 to 1926898 unknown, similar to transcriptional regulator
(MarR family)
SeqlD n° 2271 LM-4015.1 From 1771594 to 1772040 unknown, similar to putative flavodoxin
SeqlD n° 2272 LM-402.1 From 1855571 to 1855933 Unknown
SeqlD n° 2273 LM-4027.2 From 263535 to 263714 unknown, highiy similar to preprotein translocase subunit
SeqlD n° 2274 LM-4029.2 From 263844 to 264377 transcription antitermination factor
SeqlD n° 2275 LM-403.1 From 1854748 to 1855521 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 2276 LM-4031.1 From 264432 to 264902 Unknown
SeqlD n° 2277 LM-4032.1 From 265029 to 265454 ribosomal protein L11
SeqlD n° 2278 LM-4033.1 From 265494 to 266183 ribosomal protein L1
SeqlD n° 2279 LM-404.1 From 1854077 to 1854682 Unknown, similar to unknown protein
SeqlD n° 2280 LM-4040.1 From 504972 to 505277 Unknown
SeqlD n° 2281 LM-4041.1 From 552563 to 552931 Unknown, similar to unknown protein
SeqlD n° 2282 LM-4053.1 From 1270465 to 1270725 unknown
SeqlD n° 2283 LM-4055.2 From 1288261 to 1288926 unknown, weakiy similar to oligopeptide ABC transporter AppA
SeqlD n° 2284 LM-4058.1 From 1405945 to 1406226 Unknown
SeqlD n° 2285 LM-4059.1 From 1406366 to 1406713 Unknown
SeqlD n° 2286 LM-4065.1 From 2045765 to 2046046 Unknown, similar to pentitol
PTS system enzyme II B component
SeqlD n° 2287 LM-4082.1 From 2782739 to 2782894 Unknown
SeqlD n° 2288 LM-4083.1 From 2780577 to 2780798 Unknown
SeqlD n° 2289 LM-4084.1 From 2780392 to 2780574 Unknown
SeqlD n° 2290 LM-4088.1 From 2701254 to 2701445 ribosomal protein L29
SeqlD n° 2291 LM-4089.1 From 2700963 to 2701226 ribosomal protein S17
SeqlD n° 2292 LM-4090.1 From 2699383 to 2699568 ribosomal protein S14
SeqlD n° 2293 LM-4091.1 From 2697267 to 2697446 ribosomal protein L30
SeqlD n° 2294 LM-4096.1 From 367248 to 367532 Unknown
SeqlD n° 2295 LM-4097.1 From 370416 to 370634 Unknown
SeqlD n° 2296 LM-4098.1 From 389538 to 389717 Unknown, similar to conserved hypothetical protein
SeqlD n° 2297 LM-4099.1 From 401386 to 401580 unknown SeqlD n° 2298 LM-41.1 From 2738719 to 2739990 Unknwon, similar to PTS system galactitol-specific enzyme IIC component
SeqlD n° 2299 LM-4106.1 From 439009 to 439212 Unknown, similar to putative transcription regulator
SeqlD n° 2300 LM-4113.1 From 521601 to 521771 ribosomal protein L32 SeqlD n° 2301 LM-4115.1 From 616742 to 616951 Unknown, similar to unknown protein
SeqlD n° 2302 LM-4118.1 From 669086 to 669361 Unknown SeqlD n° 2303 LM-4119.1 From 696606 to 696815 unknown SeqlD n° 2304 LM-4120.1 From 707310 to 707486 unknown SeqlD n° 2305 LM-4121.1 From 709601 to 709810 unknown SeqlD n° 2306 LM-4122.1 From 731765 to 732001 Unknown, weakiy similar to flagellar switch protein
SeqlD n° 2307 LM-4123.1 From 756079 to 756291 unknown, LPXTG motif protein
SeqlD n° 2308 LM-4124.1 From 756494 to 756637 Hypothetical CDS SeqlD n° 2309 LM-4130.1 From 890069 to 890215 Unknown, hypothetical protein
SeqlD n° 2310 LM-4134.1 From 961203 to 961469 Unknown, similar to ABC transporter, ATP-binding protein (C-terminal part)
SeqlD n° 2311 LM-4135.1 From 973509 to 973670 unknown SeqlD n° 2312 LM-4137.1 From 981317 to 981544 unknown SeqlD n° 2313 LM-4139.1 From 987274 to 987501 Unknown SeqlD n° 2314 LM-4142.1 From 1003593 to 1003829 D-alanyl carrier protein SeqlD n° 2315 LM-4144.1 From 1057067 to 1057276 unknown, similar to B. subtilis YkzG protein
SeqlD n° 2316 LM-4147.1 From 1133466 to 1133696 unknown, highiy similar to
TN916 ORF8
SeqlD n° 2317 LM-4148.1 From 1145723 to 1145944 unknown, highiy similar to
TN916 ORF19
SeqlD n° 2318 LM-4149.2 From 1154864 to 1156381 unknown
SeqlD n° 2319 LM-415.1 From 1848166 to 1848690 Unknown, similar to unknown protein
SeqlD n° 2320 LM-4150.1 From 1188014 to 1188289 Unknwon, similar to carboxysome structural protein
SeqlD n° 2321 LM-4152.2 From 1287376 to 1287591 unknown, similar to transcriptional regulator
SeqlD n° 2322 LM-4153.1 From 1331439 to 1331666 unknown, similar to B. subtilis YnzC protein
SeqlD n° 2323 LM-4154.1 From 1363826 to 1363975 ribosomal protein L33
SeqlD n° 2324 LM-4155.1 From 1366220 to 1366432 unknown similar to B. subtilis yqgQ
SeqlD n° 2325 LM-4157.1 From 1385704 to 1385931 Unknown, similar to exodeoxy ribonuclease small subunit
SeqlD n° 2326 LM-4158.1 From 1387014 to 1387214 Similar to cold shock protein
SeqlD n° 2327 LM-4161.1 From 1501881 to 1502054 30S ribosomal protein S21 SeqlD n° 2328 LM-4162.2 From 1576747 to 1577055 ribosomal protein L21
SeqlD n° 2329 LM-4166.1 From 1654691 to 1654909 Unknown, hypothetical
SeqlD n° 2330 LM-417.1 From 1847472 to 1847960
Figure imgf000154_0001
SeqlD n° 2331 LM-4172.1 From 1756629 to 1756862 unknown
SeqlD n° 2332 LM-4174.1 From 1764871 to 1765077 unknown
SeqlD n° 2333 LM-4175.1 From 1769479 to 1769700 unknown
SeqlD n° 2334 LM-4179.1 From 1859050 to 1859250 ribosomal protein L35
SeqlD n° 2335 LM-418.1 From 1846355 to 1847479 phosphoribosylaminoimida zole carboxylase II
SeqlD n° 2336 LM-4180.1 From 1867180 to 1867410 Unknown, similar to unknown protein
SeqlD n° 2337 LM-4181.1 From 1881041 to 1881274 unknown, highiy similar to acyl carrier proteins
SeqlD n° 2338 LM-4182.1 From 1890951 to 1891139 ribosomal protein L28
SeqlD n° 2339 LM-4183.1 From 1902281 to 1902484 unknown
SeqlD n° 2340 LM-4184.1 From 1928579 to 1928785 unknown, similar to putative mercuric ion binding proteins
SeqlD n° 2341 LM-4186.1 From 1953513 to 1953713 similar to cold shock protein
SeqlD n° 2342 LM-4189.1 From 2094877 to 2095077 similar to major cold-shock protein
SeqlD n° 2343 LM-419.1 From 1845044 to 1846336 adenylosuccinate lyase
SeqlD n° 2344 LM-4192.1 From 2144874 to 2145065 unknown
SeqlD n° 2345 LM-4193.1 From 2150556 to 2150759 unknown
SeqlD n° 2346 LM-4195.1 From 2184120 to 2184347 Unknown
SeqlD n° 2347 LM-4197.1 From 2192379 to 2192609 Unknown
SeqlD n° 2348 LM-420.1 From 1844250 to 1844963 phosphoribosylaminoimida zole succinocarboxamide synthetase
SeqlD n° 2349 LM-4200.1 From 2234357 to 2234533 unknown
SeqlD n° 2350 LM-4201.1 From 2242097 to 2242282 unknown, similar to B. subtilis YwmG protein
SeqlD n° 2351 LM-4203.1 From 2293497 to 2293685 Unknown, similar to unknown protein
SeqlD n° 2352 LM-4206.1 From 2346593 to 2346784 unknown,
SeqlD n° 2353 LM-4207.1 From 2361582 to 2361791 unknwon
SeqlD n° 2354 LM-4208.1 From 2366288 to 2366446 protein gp22 [Bacteriophage A118]
SeqlD n° 2355 LM-4209.1 From 2387499 to 2387663 Bacteriophage A118 gp65 protein
SeqlD n° 2356 LM-421.1 From 1843993 to 1844238 Unknown, similar to unknown protein
SeqlD n° 2357 LM-4210.1 From 2388528 to 2388710 Hypothetical protein
SeqlD nD 2358 LM-4211.1 From 2389208 to 2389387 Unknown
SeqlD n° 2359 LM-42 2.1 From 2389491 to 2389658 unknown
SeqlD n° 2360 LM-4213.1 From 2391020 to 2391217 Unknown
SeqlD n° 2361 LM-4214.1 From 2394790 to 2394984 Unknown
SeqlD n° 2362 LM-4215.1 From 2395586 to 2395801 gp44 [Bacteriophage A118] SeqlD n° 2363 LM-4216.1 From 2398287 to 2398529 Unknown, similar to transcription regulator SeqlD n° 2364 LM-422.1 From 1843306 to 1843989 Unknown, similar to phosphoribosylformylglycin amidine synthetase II
SeqlD n° 2365 LM-4225.1 From 2467388 to 2467621 Unknown, similar to B. subtilis YuzB protein
SeqlD n° 2366 LM-4226.1 From 2468545 to 2468703 Unknown SeqlD n° 2367 LM-4227.1 From 2480325 to 2480528 Unknown, similar to repressor protein
SeqlD n° 2368 LM-4228.1 From 2501306 to 2501470 Unknown SeqlD n° 2369 LM-423.1 From 1841094 to 1843313 phosphoribosylformylglycin amidine synthetase I
SeqlD n° 2370 LM-4230.1 From 2559617 to 2559817 Unknown, similar to B. subtilis yvIC protein
SeqlD n° 2371 LM-4231.1 From 2567696 to 2567935 Unknown, similar to B. subtilis CsbA protein
SeqlD n° 2372 LM-4233.1 From 2611192 to 2611410 unknown, highiy similar to
H+-transporting ATP synthase chain c
SeqlD n° 2373 LM-4234.1 From 2624633 to 2624878 ribosomal protein L31 SeqlD n° 2374 LM-4235.1 From 2643753 to 2643938 Unknwon, similar to 4- oxalocrotonate isomerase
SeqlD n° 2375 LM-4236.1 From 2646464 to 2646664 unknown SeqlD n° 2376 LM-4246.1 From 145354 to 145560 Unknwon, hypothetical protein
SeqlD n° 2377 LM-4247.1 From 145085 to 145171 Unknwon, hypothetical protein
SeqlD n° 2378 LM-4248.1 From 136714 to 136992 Unknwon, similar to E. coli
YjdJ protein
SeqlD n° 2379 LM-4249.1 From 136469 to 136702 Unknown, similar to E. coli
Yjdl protein
SeqlD n° 2380 LM-425.1 From 1839682 to 1841109 glutamine phosphoribosylpyrophosph ate amidotransferase
SeqlD n° 2381 LM-4251.1 From 77863 to 78066 Unknown, Hypothetical
SeqlD n° 2382 LM-4253.1 From 52373 to 52534 Unknown
SeqlD n° 2383 LM-4254.1 From 50514 to 50753 30S ribosomal protein S18
SeqlD n° 2384 LM-426.1 From 1838614 to 1839663 phosphoribosylaminoimida zole synthetase
SeqlD n° 2385 LM-4262.2 From 267909 to 268949 Unknown, similar to penicillinase antirepressor
SeqlD n° 2386 LM-4267.1 From 1153010 to 1153783 unknown, similar to regulatory proteins
SeqlD n° 2387 LM-4268.1 From 1153848 to 1154204 unknown
SeqlD n° 2388 LM-427.1 From 1838051 to 1838617 unknown, highiy similar to phosphoribosylglycinamide formyltransferases
SeqlD n° 2389 LM-4276.2 From 2943569 to 2943703 ribosomal protein L34 SeqlD n° 2390 LM-4277.1 From 2693947 to 2694060 ribosomal protein L36 SeqlD n° 2391 LM-428.1 From 1836516 to 1838045 Bifunctional phosphoribosylaminoimida zole carboxy formyl formyltransferase and inosine-monophosphate cyclohydrolase
SeqlD n° 2392 LM-429.1 From 1835229 to 1836491 phosphoribosylglycinamide synthetase
SeqlD n° 2393 LM-4293.1 From 2130228 to 2130401 ribosomal protein L32
SeqlD n° 2394 LM-4295.1 From 2052696 to 2052833 Unknown
SeqlD n° 2395 LM-43.1 From 2740052 to 2740333 Unknwon, similar to PTS system galactitol-specific enzyme MB component
SeqlD n° 2396 LM-430.1 From 1834795 to 1835091 Unknown, similar to unknown protein
SeqlD n° 2397 LM-432.1 From 1834523 to 1834777 Unknown SeqlD n° 2398 LM-433.1 From 1833125 to 1834480 Unknown, similar to putative sodium-dependent transporter
SeqlD n° 2399 LM-4342.1 From 2317644 to 2317841 Unknown, similar to unknown protein
SeqlD n° 2400 LM-4349.1 From 1287710 to 1288243 Unknown
SeqlD n° 2401 LM-435.1 From 1832242 to 1832919 Unknown, similar to unknown protein
SeqlD n° 2402 LM-4352.1 From 436161 to 436352 Unknown
SeqlD n° 2403 LM-4353.1 From 2250846 to 2251004 Unknown
SeqlD n° 2404 LM-4358.1 From 2491285 to 2491473 unknown
SeqlD n° 2405 LM-4359.1 From 2173635 to 2173832
SeqlD n° 2406 LM-436.2 From 1829982 to 1832177 ATP-dependent DNA helicase
SeqlD n° 2407 LM-4360.1 From 263366 to 263515
SeqlD n° 2408 LM-438.1 From 1827941 to 1829956 Unknown, similar to DNA ligase
SeqlD n° 2409 LM-439.1 From 1826829 to 1827944 Unknown, similar to unknown protein
SeqlD n° 2410 LM-44.1 From 2740390 to 2740854 Unknwon, similar to PTS system galactitol-specific enzyme IIA component
SeqlD n° 2411 LM-440.1 From 1826411 to 1826704 glutamyl-tRNA(Gln) amidotransferase (subunit
C)
SeqlD n° 2412 LM-441.1 From 1824936 to 1826387 glutamyl-tRNA(Gln) amidotransferase (subunit
A)
SeqlD n° 2413 LM-442.1 From 1823494 to 1824924 glutamyl-tRNA(Gln) amidotransferase (subunit
B)
SeqlD n° 2414 LM-443.2 From 1822421 to 1823353 Unknown, similar to unknown protein
SeqlD n° 2415 LM-444.2 From 1821518 to 1822273 Unknown SeqlD n° 2416 LM-445.1 From 1820133 to 1821494 Unknown, similar to hypothetical RNA methyltransferase
SeqlD n° 2417 LM-446.1 From 1819244 to 1819798 Unknown, similar to unknown protein SeqlD n° 2418 LM-447.2 From 1818740 to 1819213 Unknown, similar to shikimate kinase SeqlD n° 2419 LM-45.2 From 2740888 to 2742957 unknown, similar to transcriptional antiterminator (BglG family)
SeqlD n° 2420 LM-451.1 From 2435023 to 2436141 Unknown, similar to S. pyogenes RofA regulatory protein
SeqlD n° 2421 LM-452.1 From 2434627 to 2434866 Hypothetical protein SeqlD n° 2422 LM-453.1 From 2433224 to 2434618 Unknown, similar to glutamate decarboxylase
SeqlD n° 2423 LM-455.1 From 2431688 to 2433211 Unknown, similar to amino acid antiporter (acid résistance)
SeqlD n° 2424 LM-456.1 From 2430826 to 2431296 Unknown, conserved hypothetical protein SeqlD n° 2425 LM-458.1 From 2428010 to 2430793 Unknown, transmembrane protein SeqlD n° 2426 LM-460.1 From 2427045 to 2427890 Unknown, conserved hypothetical protein SeqlD n° 2427 LM-463.1 From 2426270 to 2427001 Unknown, similar to N- acetylglucosamine-6- phosphate isomerase
SeqlD n° 2428 LM-464.1 From 2425723 to 2426253 Unknown, similar to unknown protein
SeqlD n° 2429 LM-466.1 From 2424922 to 2425533 Unknown SeqlD n° 2430 LM-468.1 From 2423480 to 2424721 Unknown; Similar to multidrug résistance protein
SeqlD n° 2431 LM-469.1 From 2421878 to 2423476 Unknown, conserved hypothetical protein
SeqlD n° 2432 LM-47.2 From 2743326 to 2744138 Unknown SeqlD n° 2433 LM-471.1 From 2419797 to 2421749 Unknown, similar to putative Na+/H+ antiporter
SeqlD n° 2434 LM-472.1 From 2418871 to 2419770 Unknown, similar to LysR family transcription regulator
SeqlD n° 2435 LM-473.1 From 2418083 to 2418628 Unknown, similar to NADH- dependent FMN reductase SeqlD n° 2436 LM-475.1 From 2417531 to 2418067 Unknown, similar to B. subtilis Ytml protein SeqlD n° 2437 LM-476.1 From 2416705 to 2417514 Unknown, similar to amino acid ABC transporter
(binding protein)
SeqlD n° 2438 LM-477.1 From 2415967 to 2416683 Unknown, similar to amino acid ABC-transporter
(permease) 156
SeqlD n° 2439 LM-478.1 From 2415246 to 2415953 Unknown, similar to amino acid ABC transporter
(permease)
SeqlD n° 2440 LM-48.1 From 2744212 to 2744574 Unknown, conserved hypothetical protein SeqlD n° 2441 LM-480.1 From 2414470 to 2415249 Unknown, similar to amino acid ABC-transporter, ATP- binding protein
SeqlD n° 2442 LM-481.1 From 2413478 to 2414473 Unknown, conserved hypothetical protein SeqlD n° 2443 LM-482.1 From 2413219 to 2413485 Unknown, similar to B. subtilis Ytnl protein SeqlD n° 2444 LM-483.1 From 2411900 to 2413222 Unknown, similar to nitrilotriacetate monooxygenase
SeqlD n° 2445 LM-484.1 From 2411126 to 2411827 Unknwon, similar to 16S pseudouridylate synthase
SeqlD n° 2446 LM-485.1 From 2409875 to 2410993 Similar to kinases SeqlD n" 2447 LM-486.1 From 2408967 to 2409878 Unknown, similar to Erwinia chrysanthemi IndA protein
SeqlD n° 2448 LM-487.1 From 2408450 to 2408893 Unknown, conserved hypothetical protein
SeqlD n° 2449 LM-488.1 From 2407128 to 2408453 aminopeptidase C SeqlD n° 2450 LM-49.1 From 2744571 to 2744939 Unknown SeqlD n° 2451 LM-490.1 From 2406161 to 2406913 Unknown, similar to regulatory protein DeoR family
SeqlD n° 2452 LM-491.1 From 2405241 to 2406164 fructose- 1 -phosphate kinase SeqlD n° 2453 LM-492.1 From 2403341 to 2405239 unknown, highiy similar to phosphotransferase system (PTS) fructose- specific enzyme IIABC component
SeqlD n° 2454 LM-494.1 From 2402888 to 2403235 Unknown, similar to transcriptional regulator SeqlD n° 2455 LM-495.1 From 2402357 to 2402833 compétence transcription factor SeqlD n° 2456 LM-496.1 From 2401008 to 2402366 putative integrase [Bacteriophage A118] SeqlD n° 2457 LM-497.1 From 2400264 to 2400944 Unknown, weakiy similar to gp32_Bacteriophage A118 protein
SeqlD n° 2458 LM-498.1 From 2399542 to 2400243 Unknown, similar to protein gp33 [Bacteriophage A118] SeqlD n° 2459 LM-5.1 From 2713962 to 2714942 Unknown, similar to heptaprenyl diphosphate synthase component II
SeqlD n° 2460 LM-500.1 From 2398685 to 2399161 Unknown, similar to a putative repressor protein
[Bacteriophage A118] SeqlD n° 2461 LM-502.1 From 2397800 to 2398084 Unknown SeqlD n° 2462 LM-503.1 From 2397493 to 2397774 Unknown, similar to protein gp41 [Bacteriophage A118]
SeqlD n° 2463 LM-506.1 From 2396454 to 2397230 Unknown, similar to anti- repressor [Bacteriophage
A118]
SeqlD n° 2464 LM-507.1 From 2395798 to 2396331 gp43 [Bacteriophage A118] SeqlD n° 2465 LM-509.1 From 2394317 to 2394793 Unknown, similar to bacteriophage proteins
SeqlD n° 2466 LM-51.1 From 2744985 to 2745791 Unknown, weakiy similar to
AraC-like transcription regulator
SeqlD n° 2467 LM-510.1 From 2393613 to 2394311 Unknown SeqlD n° 2468 LM-512.1 From 2392622 to 2393596 Unknown, similar to protein gp49 [Bacteriophage A118]
SeqlD n° 2469 LM-513.1 From 2391813 to 2392625 Unknown, similar to site- specific DNA- methyltransferase
SeqlD n° 2470 LM-514.1 From 2391220 to 2391816 Unknown, similar to protein gp51 [Bacteriophage A118]
SeqlD n° 2471 LM-517.1 From 2390580 to 2391023 Unknown, similar to a bacteriophage protein
SeqlD n° 2472 LM-518.1 From 2390113 to 2390583 Unknown SeqlD n° 2473 LM-52.1 From 2745907 to 2746377 Unknown, conserved hypothetical protein
SeqlD n° 2474 LM-520.1 From 2389655 to 2390116 Unknown SeqlD n° 2475 LM-522.1 From 2388729 to 2389211 unknown, similar to single- stranded DNA-binding protein
SeqlD n° 2476 LM-523.1 From 2388179 to 2388583 Unknown SeqlD n° 2477 LM-524.1 From 2387792 to 2388175 unknown SeqlD n° 2478 LM-525.1 From 2387046 to 2387480 Protein gp66
[Bacteriophage A118]
SeqlD n° 2479 LM-527.1 From 2386141 to 2386680 Unknown SeqlD n° 2480 LM-529.1 From 2385301 to 2386095 Unknown, similar to putative terminase small subunit from Bacteriophage
A118
SeqlD n° 2481 LM-53.1 From 2746423 to 2746878 Unknown, similar to ribose
5-phosphate epimerase SeqlD n° 2482 LM-530.1 From 2384001 to 2385332 putative terminase large subunit from Bacteriophage
A118
SeqlD n° 2483 LM-531.1 From 2382228 to 2383988 putative portai protein
[Bacteriophage A118] SeqlD n° 2484 LM-532.1 From 2381088 to 2382227 Protein gp4 [Bacteriophage
A118] SeqlD n° 2485 LM-533.1 From 2380419 to 2381009 Unknown, putative scaffolding protein
[Bacteriophage A118]
SeqlD n° 2486 LM-535.1 From 2379418 to 2380419 Unknown, similar to coat protein [Bacteriophage SPP1]
SeqlD n° 2487 LM-536.1 From 2379004 to 2379399 Protein gp8 [Bacteriophage
A118]
SeqlD n° 2488 LM-537.1 From 2378642 to 2379004 Protein gp9 [Bacteriophage
A118]
SeqlD n° 2489 LM-538.1 From 2378304 to 2378642 Protein gp10
[Bacteriophage A118]
SeqlD n° 2490 LM-539.1 From 2377897 to 2378304 Portein gp11 [Bacteriophage A118]
SeqlD n° 2491 LM-54.1 From 2747073 to 2747414 Unknown SeqlD n° 2492 LM-540.1 From 2377460 to 2377894 major tail shaft protein [Bacteriophage A118]
SeqlD n° 2493 LM-541.1 From 2377198 to 2377530 Portein gp13 [Bacteriophage A118]
SeqlD n° 2494 LM-542.1 From 2376721 to 2377143 Protein gp14 [Bacteriophage A118]
SeqlD n° 2495 LM-543.1 From 2376110 to 2376715 Protein gp15
[Bacteriophage A118]
SeqlD n° 2496 LM-548.1 From 2370736 to 2376099 Unknown, putative tape- measure [Bacteriophage
A118]
SeqlD n° 2497 LM-549.1 From 2369916 to 2370734 Protein gp17
[Bacteriophage A118]
SeqlD n° 2498 LM-55.1 From 2747427 to 2748683 Unknown, similar to UV- damage repair protein
SeqlD n° 2499 LM-550.1 From 2368882 to 2369907 Protein gp18 [Bacteriophage A118]
SeqlD n° 2500 LM-552.1 From 2367853 to 2368881 Protein gp19 [Bacteriophage A118]
SeqlD n° 2501 LM-553.1 From 2366780 to 2367853 protein gp20 [Bacteriophage A118]
SeqlD n° 2502 LM-554.1 From 2366451 to 2366768 protein gp21 [Bacteriophage A118]
SeqlD n° 2503 LM-555.1 From 2365893 to 2366258 protein gp23
[Bacteriophage A118]
SeqlD n° 2504 LM-557.1 From 2365599 to 2365880 holin [Bacteriophage A118] SeqlD n° 2505 LM-558.1 From 2364754 to 2365599 L-alanoyl-D-glutamate peptidase
SeqlD n° 2506 LM-559.1 From 2363709 to 2364260 Unknown SeqlD n° 2507 LM-56.1 From 2748685 to 2749497 Unknown, similar to hydrolase (esterase)
SeqlD n° 2508 LM-560.1 From 2363135 to 2363632 Unknown, similar to an unknown bacteriophage protein
SeqlD n° 2509 LM-562.3 From 2362661 to 2363110 Portein gp28
[Bacteriophage A118] SeqlD n° 2510 LM-563.3 From 318 to 1673 Chromosomal réplication initiation protein DnaA SeqlD n° 2511 LM-564.1 From 1867 to3012 DNA polymérase III, beta chain SeqlD n° 2512 LM-565.1 From 3121 to 4464 Unknown, conserved hypothetical protein
SeqlD n° 2513 LM-566.1 From 4644 to 4865 Unknown, similar to B. subtilis YaaA protein
SeqlD n° 2514 LM-567.1 From 4869 to 5981 RecF protein
SeqlD n° 2515 LM-57.1 From 2749538 to 2750233 Unknown, similar to two components response regulator
SeqlD n° 2516 LM-570.1 From 6030 to 7970 DNA gyrase subunit B
SeqlD n° 2517 LM-573.1 From 8065 to 10593 DNA gyrase subunit A
SeqlD n° 2518 LM-575.1 From 10728 to 12242 Unknown, similar to cardiolipin synthase
SeqlD n° 2519 LM-576.1 From 12258 to 12776 diamine N- acetyltransferase
SeqlD n° 2520 LM-578.1 From 12918 to 13886 Unknown, similar to mevalonate kinase
SeqlD n° 2521 LM-580.1 From 13843 to 14814 unknown, similar to mevalonate diphosphate decarboxylase
SeqlD n° 2522 LM-581.1 From 14795 to 15874 Unknown, similar to mevalonate kinases
SeqlD n° 2523 LM-582.1 From 16219 to 17325 AA3-600 quinol oxidase subunit II
SeqlD n° 2524 LM-584.1 From 17344 to 19323 AA3-600 quinol oxidase subunit I
SeqlD n° 2525 LM-585.1 From 19311 to 19922 AA3-600 quinol oxidase subunit III
SeqlD n° 2526 LM-586.1 From 19924 to 20256 unknown, highiy similar to quinol oxidase aa3-600 chain IV
SeqlD n° 2527 LM-587.1 From 20308 to 21426 Unknown, similar to
Bacillus anthracis CapA protein (polyglutamate capsule biosynthesis)
SeqlD n° 2528 LM-589.1 From 21652 to 23085 beta-glucosidase
SeqlD n° 2529 LM-591.1 From 23132 to 23953 Unknown
SeqlD n" 2530 LM-592.1 From 24194 to 24934 Unknown, similar to transcriptional regulator
(GntR family)
SeqlD n° 2531 LM-593.1 From 24950 to 25351 Unknown, similar to PTS system, fructose-specific
HA component
SeqlD n° 2532 LM-594.1 From 25351 to 25839 Unknown, similar to PTS system, fructose-specific
IIB component
SeqlD n° 2533 LM-596.1 From 25862 to 26665 Unknown, similar to PTS system, fructose-specific
IIC component
SeqlD n° 2534 LM-597.1 From 26640 to 27467 Unknown, similar to PTS system, mannose-specific
IID component SeqlD n° 2535 LM-598.1 From 27495 to 28199 Unknown, similar to phosphoheptose isomerase
SeqlD n° 2536 LM-599.1 From 28254 to 28895 Unknown, similar to E. coli copper homeostasis protein CutC
SeqlD n° 2537 LM-6.1 From 2715023 to 2716363 Unknown SeqlD n° 2538 LM-600.1 From 29100 to 31004 Unknown, similar to PTS system, beta-glucosides spécifie IIABC component
SeqlD n° 2539 LM-601.1 From 31088 to 31963 Unknown, similar to E. coli microcin C7 self-immunity protein (MccF)
SeqlD n° 2540 LM-602.1 From 32197 to 32535 Unknown
SeqlD n° 2541 LM-604.1 From 32571 to 33380 Unknown, conserved hypothetical protein
SeqlD n° 2542 LM-605.1 From 33397 to 34452 Unknown, transcriptional regulator Lacl family
SeqlD n° 2543 LM-606.1 From 34654 to 35619 Unknown, similar to xylose repressor
SeqlD n° 2544 LM-608.1 From 35616 to 38018 Unknown, similar to endoglucanase
SeqlD n° 2545 LM-609.1 From 38031 to 39383 Unknown, similar to PTS system, cellobiose-specific
IIC component
SeqlD n° 2546 LM-610.1 From 39385 to 40470 Unknown, similar to
Glucosamine-fructose-6- phosphate aminotransferase (C- terminal domain)
SeqlD n° 2547 LM-611.1 From 40705 to 41730 Unknown, similar to ornithine carbamoyltransferase
SeqlD n° 2548 LM-613.1 From 41803 to 43188 Unknown, similar to amino acid transporter
SeqlD n° 2549 LM-614.1 From 43175 to 44266 Unknown, conserved hypothetical protein
SeqlD n° 2550 LM-615.1 From 44282 to 45223 carbamate kinase
SeqlD n° 2551 LM-616.1 From 45325 to 46434 Unknown, conserved hypothetical protein
SeqlD n° 2552 LM-617.1 From 46451 to 47230 Unknown, conserved hypothetical protein, hypothetical regulator
SeqlD n° 2553 LM-618.1 From 47335 to 47994 Unknown, similar to E. coli
DedA protein
SeqlD n° 2554 LM-619.2 From 48074 to 49306 Unknown, similar to arginine deiminase
SeqlD n° 2555 LM-62.1 From 2750230 to 2752920 Unknown, similar to the two components sensor protein kdpD
SeqlD n° 2556 LM-620.2 From 703688 to 703951 Unknown SeqlD n° 2557 LM-621.3 From 703128 to 703691 Unknown, similar to acetyl transferase
SeqlD n° 2558 LM-622.1 From 703961 to 704338 Unknown, similar to unknown protein
SeqlD n° 2559 LM-623.1 From 704452 to 705387 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 2560 LM-624.1 From 705380 to 706150 Unknown, conserved membrane protein
SeqlD n° 2561 LM-625.1 From 706408 to 707292 Unknown, similar to oxidoreductase
SeqlD n° 2562 LM-626.1 From 707612 to 708220 Unknown
SeqlD n° 2563 LM-627.1 From 709134 to 709562 Unknown, similar to unknown protein
SeqlD n° 2564 LM-628.1 From 709829 to 710749 Unknown
SeqlD n° 2565 LM-629.1 From 711121 to 711435 Unknown
SeqlD n° 2566 LM-631.1 From 711428 to 712195 Unknown, similar to flagellar biosynthesic protein FliP
SeqlD n° 2567 LM-632.1 From 712208 to 712480 Unknown, similar to flagellar biosynthesis protein FliQ
SeqlD n° 2568 LM-633.1 From 712483 to 713244 Unknown, similar to flagellar biosynthetic protein FliR
SeqlD n° 2569 LM-635.1 From 713260 to 714306 Unknown, similar to flagellar biosynthetic protein flhB
SeqlD n° 2570 LM-636.1 From 714353 to 716428 Unknown, similar to flagella-associated protein flhA
SeqlD n° 2571 LM-637.1 From 716450 to 717673 Unknown, similar to flagellar biosynthesis protein FlhF
SeqlD n° 2572 LM-639.1 From 717670 to 718449 Unknown, similar to flagellar hook-basal body protein FlgG
SeqlD n° 2573 LM-641.1 From 718478 to 719266 Unknown, similar to chemotactic methyltransferase CheR
SeqlD n° 2574 LM-642.1 From 719291 to 719626 Unknown SeqlD n° 2575 LM-643.1 From 719653 to 720504 Unknown, similar to motility protein (flagellar motor rotation) MotA
SeqlD n° 2576 LM-645.1 From 720464 to 721291 Unknown, similar to motility protein (flagellar motor rotation) MotB
SeqlD n° 2577 LM-647.1 From 721301 to 721801 Unknown SeqlD n° 2578 LM-648.1 From 721824 to 723737 Unknown, similar to unknown protein SeqlD n° 2579 LM-649.1 From 723750 to 724658 Unknown, similar to CheA activity-modulating chemotaxis protein CheV
SeqlD n° 2580 LM-65.1 From 2752936 to 2753508 potassium-transporting atpase c chain
SeqlD n° 2581 LM-650.3 From 724896 to 725759 flagellin protein
SeqlD n° 2582 LM-651.1 From 726034 to 726393 Chemotaxis response regulator CheY
SeqlD n° 2583 LM-652.1 From 726413 to 728269 two-component sensor histidine kinase CheA
SeqlD n° 2584 LM-653.1 From 728282 to 728581 Unknown, similar to flagellar motor switch protein fliY C-terminal part
SeqlD n° 2585 LM-654.1 From 728600 to 729010 Unknown SeqlD n° 2586 LM-657.1 From 729026 to 730072 Unknown SeqlD n° 2587 LM-658.1 From 730074 to 730496 unknown, similar to flagellar hook assembly protein
SeqlD n° 2588 LM-659.1 From 730516 to 731751 Unknown, similar to flagellar hook protein FlgE SeqlD n° 2589 LM-660.1 From 732022 to 733014 Unknown, similar to flagellar switch protein FliM
SeqlD n° 2590 LM-664.1 From 733017 to 734564 UNknown, similar to flagellar motor switch protein fliY
SeqlD n° 2591 LM-665.1 From 734570 to 735973 Unknown SeqlD n° 2592 LM-668.1 From 735996 to 737162 Unknown SeqlD n° 2593 LM-669.1 From 737269 to 737733 Unknown SeqlD n° 2594 LM-670.1 From 737743 to 738171 Unknown SeqlD n° 2595 LM-671.1 From 738191 to 739711 Unknown, similar to flagellar hook-associated protein FlgK
SeqlD n° 2596 LM-672.1 From 739723 to 740598 Unknown, similar to flagellar hook-associated protein 3 FlgL
SeqlD n° 2597 LM-674.1 From 740610 to 741899 Unknown, similar to flagellar hook-associated protein 2 FliD
SeqlD n° 2598 LM-675.1 From 741918 to 742304 Unknown, similar to hypothetical flagellar protein
SeqlD n° 2599 LM-676.1 From 742276 to 742557 Unknown SeqlD n° 2600 LM-677.1 From 742578 to 742979 Unknown, similar to flagellar basal-body rod protein flgB
SeqlD n° 2601 LM-679.1 From 742991 to 743401 Unknown, similar to flagellar basal-body rod protein flgC
SeqlD n° 2602 LM-680.1 From 743418 to 743714 Unknown, similar to flagellar hook-basal body complex protein FliE SeqlD n° 2603 LM-682.1 From 743782 to 745434 Unknown, similar to flagellar basal-body M-ring protein fliF
SeqlD n° 2604 LM-685.1 From 745437 to 746543 Unknown, similar to flagellar motor switch protein fliG
SeqlD n° 2605 LM-686.1 From 746530 to 747222 Unknown SeqlD n° 2606 LM-688.1 From 747219 to 748520 Unknwon, similar to H+- transporting ATP synthase alpha chain Flil, flagellar- specific, -
SeqlD n° 2607 LM-689.1 From 748537 to 749205 Unknown, similar to transglycosylase
SeqlD n° 2608 LM-69.1 From 2753523 to 2755568 potassium-transporting atpase b chain
SeqlD n° 2609 LM-690.1 From 749219 to 749863 Unknown SeqlD n° 2610 LM-691.1 From 749985 to 750311 Unknown, similar to unknown protein
SeqlD n° 2611 LM-692.1 From 750311 to 750634 Unknown SeqlD n° 2612 LM-693.1 From 750680 to 751327 putative fibronectin-binding protein
SeqlD n° 2613 LM-695.1 From 751598 to 753328 Unknown, similar to pyruvate oxidase
SeqlD n° 2614 LM-697.1 From 753490 to 755295 Unknown, similar to metyl- accepting chemotaxis protein
SeqlD n° 2615 LM-698.1 From 755308 to 756036 Unknown, similar to B. subtilis YvpB protein
SeqlD n° 2616 LM-699.2 From 2842413 to 2843867 Unknown, similar to beta- glucosidase
SeqlD n° 2617 LM-7.1 From 2716408 to 2717394 Unknown
SeqlD n° 2618 LM-700.1 From 2843914 to 2844216 Unknown, similar to PTS cellobiose-specific enzyme
IIB
SeqlD n° 2619 LM-702.1 From 2844249 to 2845601 Unknown, similar to PTS cellobiose-specific enzyme
IIC component
SeqlD n° 2620 LM-703.1 From 2845613 to 2846497 Unknown, similar to xylose operon regulatory protein and to glucose kinase
SeqlD n° 2621 LM-704.1 From 2846490 to 2846798 Unknown, similar to PTS cellobiose-specific enzyme
IIA
SeqlD n° 2622 LM-705.1 From 2846839 to 2847573 Unknown, similar to hypothetical transcriptional regulator
SeqlD n° 2623 LM-706.1 From 2847673 to 2848335 Unknwon SeqlD n° 2624 LM-707.2 From 2848406 to 2849536 Unknwon SeqlD n° 2625 LM-708.3 From 2849563 to 2850450 Unknown, similar to ABC transporter, ATP-binding protein SeqlD n° 2626 LM-709.2 From 2850620 to 2852950 Unknown, similar to gamma-glutamylcysteine synthetase (for the
N erminal part) and to cyanophycin synthetase
(C-terminal part)
SeqlD n° 2627 LM-710.1 From 2852988 to 2854436 Unknown, similar to beta- glucosidase SeqlD n° 2628 LM-711.2 From 2854429 to 2856282 Unknown, similar to beta- glucoside-specific enzyme IIABC
SeqlD n° 2629 LM-712.2 From 2856379 to 2857218 Unknwon, similar to transcription antiterminator SeqlD n° 2630 LM-714.1 From 2857580 to 2858203 Unknwon, similar to ABC transporter, ATP-binding protein
SeqlD n° 2631 LM-715.1 From 2858196 to 2860364 Unknown SeqlD n° 2632 LM-716.1 From 2860426 to 2860821 Unknown SeqlD n° 2633 LM-717.1 From 2861424 to 2862614 Unknown, similar to efflux protein
SeqlD n° 2634 LM-718.1 From 2862774 to 2863283 Unknown SeqlD n° 2635 LM-720.1 From 2863561 to 2864661 Unknown, similar to probable GTP-binding protein
SeqlD n° 2636 LM-721.1 From 2864813 to 2865121 Unknown, similar to cellobiose PTS enzyme IIA SeqlD n° 2637 LM-723.1 From 2865127 to 2867397 Unknown, similar to beta- glucosidase SeqlD n° 2638 LM-724.1 From 2867432 to 2867731 Unknown, similar to PTS, cellobiose-specific IIB component
SeqlD n° 2639 LM-725.1 From 2867746 to 2869041 Unknown, similar to cellobiose phosphotransferase system enzyme IIC
SeqlD n° 2640 LM-726.1 From 2869191 to 2871107 Unknown, similar to lichenan operon transcription antiterminator licR
SeqlD n° 2641 LM-727.1 From 2871318 to 2872784 catalase SeqlD n° 2642 LM-728.1 From 2872932 to 2873915 Unknown SeqlD n° 2643 LM-73.1 From 2755580 to 2757265 unknown, highiy similar to potassium-transporting atpase a chain
SeqlD n° 2644 LM-730.1 From 2873915 to 2875837 beta-glucoside-specific phosphotransferase enzyme II
SeqlD n° 2645 LM-731.2 From 2875945 to 2876775 transcription antiterminator SeqlD n° 2646 LM-733.2 From 2877160 to 2878011 Partition protein ParB homolg
SeqlD n° 2647 LM-734.1 From 2878004 to 2878765 Partition protein, ParA homolog SeqlD n° 2648 LM-735.1 From 2878989 to 2879747 Unknown SeqlD n° 2649 LM-736.1 From 2879906 to 2880205 Unknown SeqlD n° 2650 LM-737.1 From 2880217 to 2881071 Unknown, highiy similar to
B. subtilis DNA-binding protein SpoOJ-like homolog
YyaA
SeqlD n° 2651 LM-739.1 From 2881243 to 2882049 Unknown, similar to E. coli
RpiR transcription regulator SeqlD n° 2652 LM-74.1 From 2757606 to 2757911 Unknown, similar to cellobiose phosphotransferase enzyme IIB component
SeqlD n° 2653 LM-740.1 From 2882033 to 2882938 Unknown, similar to transcription regulator SeqlD n° 2654 LM-741.1 From 2882963 to 2883409 Unknown, similar to phosphotransferase system mannitol-specific enzyme HA
SeqlD n° 2655 LM-742.1 From 2883422 to 2884078 Unknown, similar to phosphatase SeqlD n° 2656 LM-744.1 From 2884141 to 2885547 Unknown, similar to phosphotransferase system mannitol-specific enzyme IIBC
SeqlD n° 2657 LM-747.1 From 2885581 to 2886582 Unknown, similar to dehydrogenase SeqlD n° 2658 LM-748.2 From 2886584 to 2887297 Unknown, similar to a putative N- acetylmannosamine-6- phosphate epimerase
SeqlD n° 2659 LM-749.2 From 2536347 to 2538581 unknown, similar to transport protein
SeqlD n° 2660 LM-750.1 From 2538588 to 2539196 Unknown, similar to transcription regulator
SeqlD n° 2661 LM-751.1 From 2539213 to 2539611 Unknown SeqlD n° 2662 LM-752.1 From 2539838 to 2540173 Unknown SeqlD n° 2663 LM-753.1 From 2540421 to 2541857 Unknown, similar to chitinase and chitin binding protein
SeqlD n° 2664 LM-754.1 From 2542014 to 2542610 ATP-dependent Clp protease proteolytic subunit SeqlD n° 2665 LM-755.1 From 2542658 to 2544049 Unknown, similar to amino acid transporter SeqlD n° 2666 LM-758.1 From 2545487 to 2546503 Unknwon, similar to NADH oxidase SeqlD n° 2667 LM-759.1 From 2546530 to 2547501 Unknown, conserved hypothetical protein SeqlD n° 2668 LM-760.1 From 2547509 to 2548477 unknown, conserved hypothetical protein SeqlD n° 2669 LM-761.1 From 2548479 to 2549354 unknown, conserved hypothetical protein SeqlD n° 2670 LM-762.1 From 2549462 to 2551192 Unknown, similar to phosphomannomutase and phosphoglucomutase
SeqlD n° 2671 LM-763.1 From 2551234 to 2552295 Unknown, similar to aldose 1-epimerase (mutarotase)
SeqlD n° 2672 LM-764.1 From 2552312 to 2553295 UDP-glucose 4-epimerase
SeqlD n° 2673 LM-765.1 From 2553414 to 2554373 thioredoxin reductase
SeqlD n° 2674 LM-766.1 From 2554452 to 2555927 Unknown
SeqlD n° 2675 LM-767.1 From 2556013 to 2556510 Unknown, similar to acetyltransferase
SeqlD n° 2676 LM-768.1 From 2556514 to 2557167 Unknown, similar to B. subtilis P-Ser-HPr phosphatase
SeqlD n° 2677 LM-769.1 From 2557211 to 2558044 unknown, highiy similar to prolipoprotein diacylglyceryl transferase
SeqlD n° 2678 LM-77.1 From 2758046 to 2759353 Unknown, similar to cellobiose phosphotransferase enzyme IIC component
SeqlD n° 2679 LM-771.1 From 2558130 to 2559068 HPr-P(Ser) kinase/phosphatase
SeqlD n° 2680 LM-772.1 From 2559255 to 2559608 Unknown, similar to B. subtilis YvID protein
SeqlD n° 2681 LM-774.1 From 2559841 to 2561052 unknown
SeqlD nα 2682 LM-776.1 From 2561120 to 2562382 Unknown, similar to B. subtilis YvlB protein
SeqlD n° 2683 LM-778.1 From 2562591 to 2565461 excinuclease ABC (subunit
A)
SeqlD n° 2684 LM-78.1 From 2759390 to 2759692 Unknown, similar to cellobiose phosphotransferase enzyme IIA component
SeqlD n° 2685 LM-780.1 From 2565469 to 2567445 excinuclease ABC (subunit
B)
SeqlD n" 2686 LM-781.1 From 2567956 to 2568603 Unknown
SeqlD nc ' 2687 LM-782.1 From 2568625 to 2569011 unknown
SeqlD n° 2688 LM-783.1 From 2569117 to 2569422 Unknown, similar to transcription regulator ArsR family
SeqlD n° 2689 LM-784.1 From 2569690 to 2570349 Unknown, similar to négative regulator of phosphate regulon
SeqlD nc ' 2690 LM-786.1 From 2570362 to 2571141 unknown, similar to phosphate ABC transporter
(ATP-binding protein)
SeqlD nc ' 2691 LM-788.1 From 2571156 to 2571971 unknown, similar to phosphate ABC transporter
(ATP-binding protein)
SeqlD nc ' 2692 LM-789.1 From 2571999 to 2572883 Unknown, similar to phosphate ABC transporter
(permease protein) SeqlD n° 2693 LM-791.1 From 2572880 to 2573803 Unknown, similar to phosphate ABC transporter
(permease protein)
SeqlD n° 2694 LM-793.1 From 2573897 to 2574805 Unknown, similar to phosphate ABC transporter
(binding protein)
SeqlD n° 2695 LM-794.1 From 2575061 to 2576836 two-component sensor histidine kinase
SeqlD n° 2696 LM-795.1 From 2576836 to 2577546 two-component response regulator
SeqlD n° 2697 LM-796.2 From 2577696 to 2578871 Unknown
SeqlD n° 2698 LM-797.2 From 2578899 to 2580347 Unknown, similar to cardiolipin synthase
SeqlD n° 2699 LM-799.2 From 2278639 to 2279292 compétence négative regulator mecA
SeqlD n° 2700 LM-8.1 From 2717422 to 2718234 Unknown
SeqlD n° 2701 LM-80.1 From 2760147 to 2760680 unknown
SeqlD n° 2702 LM-801.1 From 2277398 to 2278513 Unknown, similar to a putative compétence protein from streptococcus pneumoniae
SeqlD n° 2703 LM-803.1 From 2275520 to 2277325 Unknown, similar to oligoendopeptidase
SeqlD n° 2704 LM-804.1 From 2274961 to 2275221 Unknown
SeqlD n° 2705 LM-805.1 From 2274127 to 2274750 Unknown
SeqlD n° 2706 LM-806.1 From 2272403 to 2274112 Unknown
SeqlD n° 2707 LM-807.1 From 2271444 to 2272316 Unknown, similar to ferrichrome ABC transporter (binding protein)
SeqlD n° 2708 LM-809.1 From 2270465 to 2271454 Unknown, similar to ferrichrome ABC transporter (permease)
SeqlD n° 2709 LM-81.1 From 2760845 to 2761954 Unknown, simlilar to cell division protein FtsW
SeqlD n° 2710 LM-810.1 From 2269705 to 2270484 Unknown, similar to ferrichrome ABC transporter (ATP-binding protein)
SeqlD n° 2711 LM-812.1 From 2268961 to 2269701 Unknown, similar to unknown protein
SeqlD n° 2712 LM-813.2 From 2268424 to 2268900 Unknown, similar to unknown protein
SeqlD n° 2713 LM-817.1 From 2259366 to 2259713 Unknown, similar to unknown protein
SeqlD n° 2714 LM-818.1 From 2258697 to 2259260 Unknown, similar to transcriptional regulator
(tetR family)
SeqlD n° 2715 LM-820.1 From 2257754 to 2258515 Unknown, similar to dehydrogenase
SeqlD n° 2716 LM-821.1 From 2256507 to 2257580 Unknown, similar to unknown proteins SeqlD n° 2717 LM-823.1 From 2255034 to 2256401 Unknown, similar to sigma-
54-dependent transcriptional activator
SeqlD n° 2718 LM-826.1 From 2253221 to 2254804 Unknown, similar to propionate CoA- transferase
SeqlD n° 2719 LM-827.1 From 2252001 to 2253224 Unknown, similar to antiporter proteins
SeqlD n° 2720 LM-83.1 From 2761951 to 2763081 Unknown, simlilar to cell division protein FtsW
SeqlD n° 2721 LM-830.1 From 2251050 to 2251979 Unknown, similar to unknown proteins
SeqlD n° 2722 LM-831.1 From 2250431 to 2250820 Unknown, similar to glyoxalase I
SeqlD n° 2723 LM-833.1 From 2249683 to 2250300 Unknown, similar to unknown proteins
SeqlD n° 2724 LM-834.1 From 2248946 to 2249617 Unknown SeqlD n° 2725 LM-835.1 From 2247998 to 2248693 Unknown, similar to transcription regulator
CRP/FNR family
SeqlD n° 2726 LM-836.1 From 2247050 to 2247928 Unknown, similar to transcriptional regulator
(AraC/XylS family)
SeqlD n° 2727 LM-837.1 From 2245899 to 2246975 Unknown, similar to oxidoreductase
SeqlD n° 2728 LM-838.1 From 2245143 to 2245883 Unknown, similar to unknown proteins
SeqlD n° 2729 LM-839.1 From 2244419 to 2245141 Unknown SeqlD n° 2730 LM-840.1 From 2243448 to 2244416 Unknown, similar to unknown proteins
SeqlD n° 2731 LM-841.1 From 2242376 to 2243425 Unknown, similar to oxidoreductase
SeqlD n° 2732 LM-842.1 From 2240068 to 2241969 Unknown SeqlD n° 2733 LM-843.1 From 2239672 to 2240013 Unknown SeqlD n° 2734 LM-845.1 From 2236844 to 2239135 Unknown, similar to ribonucleoside-diphosphate reductase, subunit alpha
SeqlD n° 2735 LM-846.1 From 2235739 to 2236788 Unknown, similar to ribonucleoside-diphosphate reductase, subunit beta
SeqlD n° 2736 LM-847.1 From 2235305 to 2235742 Unknown, similar to flavodoxin SeqlD n° 2737 LM-848.1 From 2234982 to 2235308 Unknown, similar to thioredoxin SeqlD n° 2738 LM-849.1 From 2234575 to 2234874 Unknown, similar to unknown proteins SeqlD n° 2739 LM-85.1 From 2763082 to 2765652 Unknown, highiy similar to
Mg2+ transport ATPase SeqlD n° 2740 LM-850.1 From 2233841 to 2234158 Unknown, similar to unknown proteins SeqlD n° 2741 LM-851.1 From 2233150 to 2233791 Unknown, similar to unknown proteins SeqlD n° 2742 LM-853.1 From 2232087 to 2233094 Unknown, similar to unknown proteins SeqlD n° 2743 LM-854.1 From 2231127 to 2231981 Unknown, similar to transcription regulator LysR family
SeqlD n° 2744 LM-856.1 From 2230042 to 2231058 Unknown, similar to unknown protein SeqlD n° 2745 LM-857.1 From 2229193 to 2229927 Unknown, similar to transcription regulator
GntR family
SeqlD n° 2746 LM-858.1 From 2227412 to 2229154 Unknown, weakiy similar to mannose-6-phosphate isomerase
SeqlD n° 2747 LM-859.1 From 2226533 to 2227198 Unknown SeqlD n° 2748 LM-860.1 From 2225471 to 2225944 Unknown, similar to unknown protein
SeqlD n° 2749 LM-861.1 From 2224219 to 2225454 Unknown, similar to ABC transporter (membrane protein)
SeqlD n° 2750 LM-862.1 From 2223324 to 2224226 Unknown, similar to ABC transporter (ATP-binding protein)
SeqlD n° 2751 LM-863.1 From 2221671 to 2223128 Unknown, similar to transcription regulator SeqlD n° 2752 LM-865.1 From 2221186 to 2221659 Unknown, similar to PTS system, fructose-specific enzyme IIA component
SeqlD n° 2753 LM-866.1 From 2220862 to 2221173 Unknown, similar to PTS system, fructose-specific enzyme IIB component
SeqlD n° 2754 LM-867.1 From 2219751 to 2220845 Unknown, similar to PTS system, fructose-specific enzyme IIC component
SeqlD n° 2755 LM-869.1 From 2218879 to 2219733 Unknown, similar to fructose-1 ,6-biphosphate aldolase type II
SeqlD n° 2756 LM-87.1 From 2766207 to 2766773 Unknown, similar to transcription regulator,
TetR family
SeqlD n° 2757 LM-870.1 From 2217963 to 2218862 Unknown, similar to fructose-1 ,6-biphosphate aldolase type II
SeqlD n° 2758 LM-871.1 From 2217222 to 2217953 Unknown SeqlD n° 2759 LM-872.1 From 2216054 to 2216743 Unknown SeqlD n° 2760 LM-874.2 From 2486136 to 2486921 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 2761 LM-875.1 From 2484816 to 2486117 Unknown, similar to aminotransferase SeqlD n° 2762 LM-876.1 From 2483589 to 2484815 Unknown, similar to aminotransferase SeqlD n° 2763 LM-877.1 From 2483149 to 2483592 Unknown, similar to conserved hypothetical proteins
SeqlD n° 2764 LM-878.1 From 2481732 to 2483126 Unknown, similar to conserved hypothetical proteins
SeqlD n° 2765 LM-879.1 From 2481023 to 2481574 Unknown SeqlD n° 2766 LM-880.1 From 2480531 to 2480944 Unknown SeqlD n° 2767 LM-881.1 From 2479607 to 2479921 unknown SeqlD n° 2768 LM-883.1 From 2478623 to 2479465 unknown, similar to B. subtilis YunF protein
SeqlD n° 2769 LM-884.1 From 2478225 to 2478590 Unknown SeqlD n° 2770 LM-885.1 From 2477385 to 2478224 Unknown, similar to conserved hypothetical proteins
SeqlD n° 2771 LM-886.1 From 2475860 to 2477251 Unknown, similar to B. subtilis YunD protein SeqlD n° 2772 LM-887.1 From 2475588 to 2475863 Unknown, similar to B. subtilis YutD protein SeqlD n° 2773 LM-888.1 From 2474801 to 2475568 Unknown, similar to conserved hypothetical protein and to B. subtilis
YutF protein
SeqlD n° 2774 LM-889.1 From 2474309 to 2474752 Unknown, similar to acetyltransferase SeqlD n° 2775 LM-89.1 From 2766935 to 2768707 Unknown, similar to autolysin, N- acetylmuramidase
SeqlD n° 2776 LM-890.1 From 2472963 to 2474273 Unknown, similar to conserved hypothetical proteins
SeqlD n° 2777 LM-892.1 From 2472438 to 2472938 Unknown, low température requirement C protein, also similar to B. subtilis YutG protein
SeqlD n° 2778 LM-893.1 From 2472089 to 2472325 Unknown, similar to NifU protein SeqlD n° 2779 LM-896.1 From 2468210 to 2468392 Unknown, hypothetical
CDS SeqlD n° 2780 LM-897.1 From 2467643 to 2467969 Unknown, similar to B. subtilis YuzD protein SeqlD n° 2781 LM-899.1 From 2466713 to 2467342 Unknown, conserved hypothetical protein similar to B. subtilis YhfK protein
SeqlD n° 2782 LM-90.1 From 2768944 to 2769273 Unknown SeqlD n° 2783 LM-901.1 From 2465630 to 2466625 Unknown, similar to hypothetical thioredoxine reductase
SeqlD n° 2784 LM-903.1 From 2463969 to 2465180 Unknown, similar to NADH dehydrogenase SeqlD n° 2785 LM-904.1 From 2463066 to 2463884 Unknown, similar to B. subtilis YwqG protein SeqlD n° 2786 LM-906.1 From 2461803 to 2463029 Unknown, conserved hypothetical protein
SeqlD n° 2787 LM-907.1 From 2461219 to 2461692 Unknown, similar to B. subtilis YuiD protein
SeqlD n° 2788 LM-908.1 From 2460720 to 2461091 Unknown, similar to B. subtilis YuxO protein
SeqlD n° 2789 LM-91.1 From 2769381 to 2770007 Unknown, similar to thymidylate kinase
SeqlD n° 2790 LM-910.1 From 2460090 to 2460683 unknown, similar to proteins involved in résistance to cholate and to
NA(+) and in pH homeostasis
SeqlD n° 2791 LM-911.1 From 2459819 to 2460106 unknown, similar to proteins involved in résistance to cholate and to
NA(+) and in pH homeostasis
SeqlD n° 2792 LM-912.1 From 2459343 to 2459822 unknown, similar to proteins involved in résistance to cholate and to
NA(+) and in pH homeostasis
SeqlD n° 2793 LM-913.1 From 2457852 to 2459336 unknown, similar to proteins involved in résistance to cholate and to
NA(+) and in pH homeostasis
SeqlD n° 2794 LM-915.1 From 2457512 to 2457859 unknown, similar to proteins involved in résistance to cholate and to
NA(+) and in pH homeostasis
SeqlD n° 2795 LM-916.1 From 2457087 to 2457512 unknown, similar to proteins involved in résistance to cholate and to
NA(+) and in pH homeostasis
SeqlD n° 2796 LM-917.1 From 2454695 to 2457103 unknown, similar to proteins involved in résistance to cholate and to
NA(+) and in pH homeostasis
SeqlD n° 2797 LM-918.1 From 2453138 to 2454349 Unknown, similar to multi- drug résistance efflux pump
SeqlD n° 2798 LM-919.1 From 2452322 to 2452906 Unknown, similar to peptidyl-prolyl cis-trans isomerase
SeqlD n° 2799 LM-92.1 From 2770060 to 2771439 Unknown, similar to lysine decarboxylase
SeqlD n° 2800 LM-920.1 From 2451840 to 2452235 Unknown SeqlD n° 2801 LM-922.1 From 2450437 to 2451798 Unknown, similar to aspartate kinase SeqlD n° 2802 LM-923.1 From 2449878 to 2450192 Unknown, similar to phosphotransferase system (PTS) beta- glucoside-specific enzyme IIB component
SeqlD n° 2803 LM-924.1 From 2449155 to 2449835 unknown, similar to ABC- transporter ATP binding proteins
SeqlD n° 2804 LM-925.1 From 2448052 to 2449155 Unknown, similar to putative ABC-transporter transmembrane subunit
SeqlD n° 2805 LM-926.1 From 2446747 to 2447913 Unknown, similar to aminotransferase
SeqlD n° 2806 LM-927.1 From 2446240 to 2446593 Unknown, similar to B. subtilis gênerai stress protein 13 containing a ribosomal S1 protein domain
SeqlD n° 2807 LM-929.1 From 2445685 to 2446092 Unknown SeqlD n° 2808 LM-930.2 From 2444231 to 2445583 glucose-6-phosphate isomerase
SeqlD n° 2809 LM-931.2 From 2443368 to 2444126 Unknown, similar to transcription regulator
DeoR family
SeqlD n° 2810 LM-935.1 From 198201 to 199409 Unknown, similar to ABC transporter, ATP-binding protein
SeqlD n° 2811 LM-936.1 From 197512 to 198204 ABC transporter, ATP- binding protein
SeqlD n° 2812 LM-937.1 From 196786 to 197463 unknwon SeqlD n° 2813 LM-938.1 From 195793 to 196611 Unknown, similar to PurR, transcription repressor of purine operon of B. subtilis
SeqlD n° 2814 LM-939.1 From 194892 to 195629 Unknwon, similar to a putative phospho-beta- glucosidase
SeqlD n° 2815 LM-94.1 From 2771623 to 2772612 Unknown, similar to dihydroxyacetone kinase
SeqlD n° 2816 LM-941.1 From 193989 to 194870 Unknwon, similar to B. subtilis YabH protein
SeqlD n° 2817 LM-942.1 From 193593 to 193850 Unknwon, highiy similar to B. subtilis Veg protein
SeqlD n° 2818 LM-943.1 From 192586 to 193473 dimethyladenosine transferase (16S rRNA dimethylase)
SeqlD n° 2819 LM-945.1 From 192018 to 192593 Unkwnon, similar to B. subtilis YabF protein
SeqlD n° 2820 LM-946.1 From 190690 to 191916 Unknwon, similar to B. subtilis YabE protein SeqlD n° 2821 LM-947.1 From 189625 to 190398 Unknwon, similar to conserved hypothetical proteins
SeqlD n° 2822 LM-949.1 From 187867 to 189528 Unknwon, similar to oligo-
1,6-glucosidase SeqlD n° 2823 LM-95.1 From 2772634 to 2773230 Unknown, similar to hypothetical dihydroxyacetone kinase
SeqlD n° 2824 LM-950.1 From 185572 to 187863 Unknwon, similar to alpha- glucosidase SeqlD n° 2825 LM-955.1 From 182267 to 185569 Unknwon, similar to alpha- xylosidase and alpha- glucosidase
SeqlD n° 2826 LM-958.1 From 180928 to 182184 Unknwon, similar to sugar
ABC transporter, periplasmic sugar-binding protein
SeqlD n° 2827 LM-96.1 From 2773234 to 2773608 unknown SeqlD n° 2828 LM-960.1 From 180052 to 180900 Unknwon, similar to sugar
ABC transporter, permease protein
SeqlD n° 2829 LM-961.1 From 179174 to 180052 Unknwon, similar to sugar
ABC transporters, permease proteins
SeqlD n° 2830 LM-962.1 From 177924 to 179138 Unknwon, similar to xylose repressor
SeqlD n° 2831 LM-964.1 From 175766 to 177760 methionyl-tRNA synthetase SeqlD n° 2832 LM-965.1 From 174834 to 175694 Unknwon, similar to glucose uptake protein
SeqlD n° 2833 LM-968.1 From 172903 to 173208 Unknown, similar to transposase
SeqlD n° 2834 LM-969.1 From 172410 to 172802 Unknown, similar to transposase (N-terminal part)
SeqlD n° 2835 LM-97.1 From 2773714 to 2774565 Unknown, similar to putative transcription regulator
SeqlD n° 2836 LM-970.1 From 172072 to 172410 Unknown, similar to transposase C-terminal part
SeqlD n° 2837 LM-975.1 From 168005 to 169270 Unknwon SeqlD n° 2838 LM-977.1 From 167086 to 167943 Unknown, similar to a glucose uptake protein
SeqlD n° 2839 LM-978.1 From 166687 to 166971 Unknown, similar to B. subtilis transcription regulatory protein AbrB SeqlD n° 2840 LM-98.1 From 2774684 to 2775523 Unknown, similar to conserved hypothetical protein SeqlD n° 2841 LM-981.1 From 165761 to 166642 Unknwon, conserved hypothetical protein SeqlD n° 2842 LM-982.1 From 165492 to 165764 unknwon, similar to B. subtilis YazA protein
SeqlD n° 2843 LM-983.1 From 164756 to 165508 unknwon, conserved hypothetical protein
SeqlD n° 2844 LM-984.1 From 164309 to 164698 Unknwon, similar to B. subtilis YabA protein
SeqlD n° 2845 LM-987.1 From 163465 to 164298 Unknwon
SeqlD n° 2846 LM-988.2 From 162467 to 163459 Unknwon, similar to B. subtilis DNA polymérase III
(delta subunit)
SeqlD n° 2847 LM-990.2 From 596580 to 597620 Unknown, conserved hypothetical protein
SeqlD n° 2848 LM-991.1 From 595843 to 596538 Unknown, similar to phosphoglycerate mutase
SeqlD n° 2849 LM-992.1 From 595135 to 595842 Unknown, similar to phosphoglycerate mutase
SeqlD n° 2850 LM-994.1 From 593528 to 595006 Unknown, similar to di- tripeptide ABC transporter
(membrane protein)
SeqlD n° 2851 LM-995.1 From 592257 to 593438 Unknown, similar to NADH- dependent butanol dehydrogenase
SeqlD n° 2852 LM-996.1 From 591412 to 592047 Unknown SeqlD n° 2853 LM-997.1 From 590324 to 591355 Unknown, similar to unknown protein
SeqlD n° 2854 LM-998.1 From 589406 to 590248 Unknown

Claims

REVENDICATIONS
1. Séquence nucléotidique de Listeria monocytogenes caractérisée en ce qu'elle correspond à SEQ ID N° 1.
2. Séquence nucléotidique de Listeria monocytogenes, caractérisée en ce qu'elle est choisie parmi : a) une séquence nucléotidique comportant au moins 80 % d'identité avec SEQ ID N0 1 ; b) une séquence nucléotidique hybridant dans des conditions de forte stringence avec SEQ ID N° 1 ; c) une séquence nucléotidique complémentaire de SEQ ID N° 1 ou complémentaire d'une séquence nucléotidique telle que définie en a), ou b), ou une séquence nucléotidique de l'ARN correspondant ; d) une séquence nucléotidique de fragment représentatif de SEQ ID N° 1, ou de fragment représentatif d'une séquence nucléotidique telle que définie en a), b) ou c); e) une séquence nucléotidique comprenant une séquence telle que définie en a), b), c) ou d) ; et f) une séquence nucléotidique modifiée d'une séquence nucléotidique telle que définie en a), b), c), d) ou e).
3. Séquence nucléotidique selon la revendication 2, caractérisée en ce qu'il s'agit d'une séquence issue de SEQ ID N° 1 , et en ce qu'elle code pour un polypeptide choisi parmi les polypeptides de séquence SEQ ID N° 1 à SEQ ID N° 2854.
4. Séquence nucléotidique caractérisée en ce qu'elle comprend une séquence nucléotidique choisie parmi : a) une séquence nucléotidique selon la revendication 3 ; b) une séquence nucléotidique comportant au moins 80 % d'identité avec une séquence nucléotidique selon la revendication 3 ; c) une séquence nucléotidique s'hybridant dans des conditions de forte stringence avec une séquence nucléotidique selon la revendication 3 ; d) une séquence nucléotidique complémentaire ou d'ARN correspondant à une séquence telle que définie en a), b) ou c) ; e) une séquence nucléotidique de fragment représentatif d'une séquence telle que définie en a), b), c) ou d) ; et f) une séquence nucléotidique modifiée d'une séquence telle que définie en a), b), c), d) ou e).
5. Polypeptide codé par une séquence nucléotidique selon l'une des revendications 2 à 4.
6. Polypeptide selon la revendication 5, caractérisé en ce qu'il est choisi parmi les séquences SEQ ID N° 2 à SEQ ID N° 2854.
7. Polypeptide caractérisé en ce qu'il comprend un polypeptide choisi parmi : a) un polypeptide selon l'une des revendications 5 et 6 ; b) un polypeptide présentant au moins 80 % d'identité avec un polypeptide selon l'une des revendications 5 et 6 ; c) un fragment d'au moins 5 acides aminés d'un polypeptide selon l'une des revendications 5 et 6, ou tel que défini en b) ; d) un fragment biologiquement actif d'un polypeptide selon l'une des revendications 5 et 6, ou tel que défini en b) ou c) ; et e) un polypeptide modifié d'un polypeptide selon l'une des revendications 5 et 6 ou tel que défini en b), c) ou d).
8. Séquence nucléotidique codant pour un polypeptide selon la revendication 7.
9. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans la biosynthèse des acides aminés ou l'un de ses fragments.
10. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans la biosynthèse des cofacteurs, groupes prosthétiques et transporteurs ou l'un de ses fragments.
11. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide d'enveloppe cellulaire ou situé à la surface de Listeria monocytogenes ou l'un de ses fragments, ledit polypeptide étant de préférence SEQ ID N° 2 à SEQ ID N° 41.
12. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans la machinerie cellulaire ou l'un de ses fragments.
13. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans le métabolisme intermédiaire central ou l'un de ses fragments.
14. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans le métabolisme énergénique ou l'un de ses fragments.
15. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans le métabolisme des acides gras et des phospholipides ou l'un de ses fragments.
16. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans le métabolisme des nucléotides, des purines, des pyrimidines ou nucléosides ou l'un de ses fragments.
17. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans les fonctions de régulation ou l'un de ses fragments.
18. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans le processus de réplication ou l'un de ses fragment.
19. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans le processus de transcription ou l'un de ses fragments.
20. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans le processus de traduction ou l'un de ses fragments.
21. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans le processus de transport et de liaison des protéines ou l'un de ses fragments.
22. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans l'adaptation aux conditions atypiques ou l'un de ses fragments.
23. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans la sensibilité aux médicaments et analogues ou l'un de ses fragments.
24. Séquence nucléotidique caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans la biosynthèse de la vitamine B12, ledit polypeptide étant de préférence SEQ ID N° 42 à SEQ ID N°64.
25. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide de Listeria monocytogenes impliqué dans les fonctions relatives aux transposons ou l'un de ses fragments.
26. Séquence nucléotidique selon l'une des revendications 2 à 4, et 8, caractérisée en ce qu'elle code pour un polypeptide spécifique de Listeria monocytogenes ou l'un de ses fragments.
27. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans la biosynthèse des acides aminés ou l'un de ses fragments.
28. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans la biosynthèse des cofacteurs, groupes prosthétiques et transporteurs ou l'un de ses fragment.
29. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide d'enveloppe cellulaire ou situé à la surface de Listeria monocytogenes ou l'un de ses fragments, ledit polypeptide étant de préférence SEQ ID N° 2 à SEQ ID N° 41.
30. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans la machinerie cellulaire ou l'un de ses fragments.
31. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans le métabolisme intermédiaire central ou l'un de ses fragements.
32. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans le métabolisme énergétique ou l'un de ses fragments.
33. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans le métabolisme des acides gras et des phospholipides ou l'un de ses fragments.
34. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans le métabolisme des nucléotides, des purines, des pyrimidines ou nucléosides ou l'un de ses fragments.
35. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans les fonctions de régulation ou l'un de ses fragments.
36. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans le processus de réplication ou l'un de ses fragments.
37. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans le processus de transcription ou l'un de ses fragments.
38. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans le processus de traduction ou l'un de ses fragments.
39. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans le processus de transport et de liaison des protéines ou l'un de ses fragments.
40. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans l'adaptation aux conditions atypiques ou l'un de ses fragments.
41. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans la sensibilité aux médicaments et analogues ou l'un de ses fragments.
42. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans la biosynthèse de la vitamine B12, ledit polypeptide étant de préférence SEQ ID N° 42 à SEQ ID N°64.
43. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide de Listeria monocytogenes impliqué dans les fonctions relatives aux transposons ou l'un de ses fragments.
44. Polypeptide selon l'une des revendications 5 à 7, caractérisé en ce qu'il s'agit d'un polypeptide spécifique de Listeria monocytogenes ou l'un de ses fragments.
45. Séquence nucléotidique selon l'une des revendications 1 à 4, et 8 à 26 , et/ou séquence de polypeptide selon l'une des revendications 5 à 7, et, 27 à 44, caractérisée(s) en ce que ladite ou lesdites séquences est (sont) enregistrée(s) sur un support d'enregistrement dont la forme et la nature facilitent la lecture, l'analyse et/ou l'exploitation de ladite ou desdites séquences.
46. Séquence nucléotidique ou séquence de polypeptide selon la revendication 45, caractérisée en ce que le support d'enregistrement est un CD-ROM, une disquette informatique ou un serveur informatique.
47. Support d'enregistrement caractérisé en ce qu'y est enregistré une séquence nucléotidique ou de polypeptide selon la revendication 45 ou 46.
48. Utilisation d'un support selon la revendication 47 pour le choix d'amorces ou de sondes nucléotidiques pour la détermination de gènes dans des souches proches de Listeria monocytogenes.
49. Utilisation d'un support selon la revendication 47 pour l'étude du polymorphisme génétique de souches proches de Listeria monocytogenes.
50. Utilisation d'un support selon la revendication 47, pour l'étude d'autres génomes en particulier pour l'annotation automatique de gènes provenant d'autres génomes.
51. Séquence nucléotidique utilisable comme amorce ou comme sonde, caractérisée en ce que ladite séquence est choisie parmi les séquences nucléotidiques selon l'une des revendications 2 à 4, et, 8 à 26.
52. Séquence nucléotidique selon la revendication 51, caractérisée en ce qu'elle est marquée par un composé radioactif ou par un composé non radioactif.
53. Séquence nucléotidique selon l'une des revendications 51 et 52, caractérisée en ce qu'elle est immobilisée sur un support, de manière covalente ou non- covalente.
54. Séquence nucléotidique selon la revendication 53, caractérisée en ce qu'elle est immobilisée sur un support tel qu'un filtre à haute densité ou une puce à ADN.
55. Séquence nucléotidique selon l'une des revendications 52 à 55 pour la détection et/ou l'amplification de séquences nucléiques.
56. Puce à ADN ou filtre, caractérisé en ce qu'il contient au moins une séquence nucléotidique selon la revendication 55.
57. Puce à ADN ou filtre selon la revendication 56, caractérisé en ce qu'il contient en outre au moins une séquence nucléotidique d'un micro-organisme autre que Listeria monocytogenes, immobilisée sur le support de ladite puce.
58. Puce à ADN ou filtre selon la revendication 57, caractérisé en ce que le microorganisme autre est choisi parmi un micro-organisme associé à Listeria monocytogenes, une bactérie du genre Listeria, et un variant de Listeria monocytogenes.
59. Kit ou nécessaire pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé, caractérisé en ce qu'il comprend une puce à ADN ou un filtre selon la revendication 56.
60. Kit ou nécessaire pour la détection et/ou l'identification d'un micro-organisme, caractérisé en ce qu'il comprend une puce à ADN ou un filtre selon l'une des revendications 57 et 58.
61. Kit ou nécessaire pour la détection et/ou la quantification de l'expression d'au moins un gène de Listeria monocytogenes, caractérisé en ce qu'il comprend une puce à ADN ou un filtre selon l'une des revendications 56 à 58.
62. Vecteur de clonage, et/ou d'expression, caractérisé en ce qu'il contient une séquence nucléotidique selon l'une des revendications 2 à 4 et 8 à 26.
63. Vecteur de clonage, et/ou d'expression selon la revendication 62, caractérisé en ce qu'il contient une séquence nucléotidique selon la revendication 11.
64. Vecteur de clonage, et/ou d'expression selon la revendication 62, caractérisé en ce qu'il contient une séquence nucléotidique selon la revendication 14.
65. Vecteur de clonage, et/ou d'expression selon la revendication 62, caractérisé en ce qu'il contient une séquence nucléotidique selon la revendication 9, 19 ou 20.
66. Vecteur de clonage, et/ou d'expression selon la revendication 62, caractérisé en ce qu'il contient une séquence nucléotidique selon la revendication 12, en particulier codant pour une protéine impliquée dans les mécanismes de sécrétion.
67. Vecteur de clonage, et/ou d'expression selon la revendication 62, caractérisé en ce qu'il contient une séquence nucléotidique selon la revendication 24.
68. Vecteur de clonage, et/ou d'expression selon la revendication 62, caractérisé en ce qu'il contient une séquence nucléotidique codant pour une protéine impliquée dans la résistance et/ou l'adaptation au stress.
69. Cellule hôte, caractérisée en ce qu'elle est transformée par un vecteur selon l'une des revendications 62 à 68.
70. Cellule hôte selon la revendication 69, caractérisée en ce qu'il s'agit d'une bactérie appartenant au genre Listeria.
71. Cellule hôte selon la revendication 70, caractérisée en ce qu'il s'agit d'une bactérie appartenant à l'espèce Listeria monocytogenes.
72. Végétal ou animal, excepté l'Homme, comprenant une cellule transformée selon l'une des revendications 69 à 71.
73. Procédé de préparation d'un polypeptide, caractérisé en ce que l'on cultive une cellule transformée par un vecteur selon la revendication 62 dans des conditions permettant l'expression dudit polypeptide et que l'on récupère ledit polypeptide recombinant.
74. Polypeptide recombinant susceptible d'être obtenu par un procédé selon la revendication 73.
75. Procédé de préparation d'un polypeptide synthétique selon l'une des revendications 5 à 7, 27 à 44, caractérisé en ce que l'on effectue une synthèse chimique dudit polypeptide.
76. Polypeptide hybride, caractérisé en ce qu'il comprend au moins la séquence d'un polypeptide selon l'une des revendications 5 à 7, 27 à 44 et 74, et une séquence d'un polypeptide susceptible d'induire une réponse immunitaire chez l'homme ou l'animal.
77. Séquence nucléotidique codant pour un polypeptide hybride selon la revendication 76.
78. Vecteur caractérisé en ce qu'il contient une séquence nucléotidique selon la revendication 77.
79. Anticorps monoclonal ou polyclonal, ses fragments, ou anticorps chimérique, caractérisé en ce qu'il est capable de reconnaître spécifiquement un polypeptide selon l'une des revendications 5 à 7, 27 à 44, 74 ou 76.
80. Anticorps selon la revendication 79, caractérisé en ce qu'il s'agit d'un anticorps marqué.
81. Procédé pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un microorganisme associé dans un échantillon biologique, caractérisé en ce qu'il comprend les étapes suivantes : a) mise en contact de l'échantillon biologique avec un anticorps selon l'une des revendications 79 et 80; b) mise en évidence du complexe antigène-anticorps éventuellement formé.
82. Procédé pour la détection de l'expression d'un gène de Listeria monocytogenes caractérisé en ce que l'on met en contact une souche de Listeria monocytogenes, avec un anticorps selon la revendication 79 ou 80 et que l'on détecte le complexe antigène/anticorps éventuellement formé.
83. Kit ou nécessaire pour la mise en œuvre d'un procédé selon la revendication 81 ou 82, caractérisé en ce qu'il comprend les éléments suivants : a) un anticorps selon l'une des revendications 79 et 80; b) éventuellement, les réactifs pour la constitution du milieu propice à la réaction immunologique ; c) éventuellement, les réactifs permettant la mise en évidence des complexes antigène-anticorps produits par la réaction immunologique.
84. Polypeptide selon l'une des revendications 5 à 7, 27 à 44, 74 et 76, ou anticorps selon l'une des revendications 79 et 80, caractérisé en ce qu'il est immobilisé sur un support, notamment une puce à protéine.
85. Puce à protéine, caractérisée en ce qu'elle contient au moins un polypeptide selon l'une des revendications 5 à 7, 27 à 44, 74 et 76, ou au moins un anticorps selon l'une des revendications 79 et 80, immobilisé sur le support de ladite puce.
86. Puce à protéine selon la revendication 85, caractérisée en ce qu'elle contient en outre au moins un polypeptide de micro-organisme autre que Listeria monocytogenes ou au moins un anticorps dirigé contre un composé de micro- organisme autre que Listeria monocytogenes, immobilisé sur le support de ladite puce.
87. Kit ou nécessaire pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé, caractérisé en ce qu'il comprend une puce à protéine selon l'une des revendications 85 et
86.
88. Kit ou nécessaire pour la détection et/ou l'identification d'un micro-organisme, caractérisé en ce qu'il comprend une puce à protéine selon la revendication 86.
89. Procédé de détection et/ou d'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé dans un échantillon biologique, caractérisé en ce qu'il met en œuvre une séquence nucléotidique selon l'une des revendications 1 à 4, 8 à 26, 51 à 55 et 77.
90. Procédé selon la revendication 89, caractérisé en ce qu'il comporte les étapes suivantes : a) éventuellement, isolement de l'ADN à partir de l'échantillon biologique à analyser, ou obtention d'un ADNc à partir de l'ARN de l'échantillon biologique ; b) amplification spécifique de l'ADN de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé à l'aide d'au moins une amorce selon l'une des revendications 51 à 55 ; c) mise en évidence des produits d'amplification.
91. Procédé selon la revendication 89, caractérisé en ce qu'il comprend les étapes suivantes : a) mise en contact d'une sonde nucléotidique selon l'une des revendications 51 à 55, avec un échantillon biologique, l'acide nucléique contenu dans l'échantillon biologique ayant, le cas échéant, préalablement été rendu accessible à l'hybridation, dans des conditions permettant l'hybridation de la sonde à l'acide nucléique d'une bactérie appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé ; b) mise en évidence de l'hybride éventuellement formé entre la sonde nucléotidique et l'acide nucléique de l'échantillon biologique.
92. Procédé selon la revendication 89, caractérisé en ce qu'il comprend les étapes suivantes : a) mise en contact d'une sonde nucléotidique immobilisée sur un support selon la revendication 53 avec un échantillon biologique, l'acide nucléique de l'échantillon ayant, le cas échéant, été préalablement rendu accessible à l'hybridation, dans des conditions permettant l'hybridation de la sonde à l'acide nucléique d'une bactérie appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé ; b) mise en contact de l'hybride formé entre la sonde nucléotidique immobilisée sur un support et l'acide nucléique contenu dans l'échantillon biologique, le cas échéant après élimination de l'acide nucléique de l'échantillon biologique n'ayant pas hybride avec la sonde, avec une sonde nucléotidique marquée selon la revendication 52 ; c) mise en évidence du nouvel hybride formé à l'étape b).
93. Procédé selon la revendication 92, caractérisé en ce que, préalablement à l'étape a), l'ADN de l'échantillon biologique ou l'ADNc obtenu éventuellement par transcription inverse de l'ARN de l'échantillon, est amplifié à l'aide d'au moins une amorce selon l'une des revendications 51 à 55.
94. Kit ou nécessaire pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé, caractérisé en ce qu'il comprend les éléments suivants : a) une sonde nucléotidique selon l'une des revendications 51 à 55; b) éventuellement, les réactifs nécessaires à la mise en œuvre d'une réaction d'hybridation ; c) éventuellement, au moins une amorce selon l'une des revendications 51 à 55 ainsi que les réactifs nécessaires à une réaction d'amplification de l'ADN.
95. Kit ou nécessaire pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé, caractérisé en ce qu'il comprend les éléments suivants : a) une sonde nucléotidique, dite sonde de capture, selon la revendication 53 ; b) une sonde oligonucléotidique, dite sonde de révélation, selon la revendication 52; c) éventuellement, au moins une amorce selon l'une des revendications 51 à 55 ainsi que les réactifs nécessaires à une réaction d'amplification de l'ADN.
96. Kit ou nécessaire pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes ou à un micro-organisme associé, caractérisé en ce qu'il comprend les éléments suivants : a) au moins une amorce selon l'une des revendications 51 à 55; b) éventuellement, les réactifs nécessaires pour effectuer une réaction d'amplification d'ADN ; c) éventuellement, un composant permettant de vérifier la séquence du fragment amplifié, plus particulièrement une sonde oligonucléotidique selon l'une des revendications 51 à 55.
97. Procédé selon les revendications 81, 82 et 89 à 93 ou kit ou nécessaire selon les revendications 83, 87, 88 et 94 à 96 pour la détection et/ou l'identification de bactéries appartenant à l'espèce Listeria monocytogenes, caractérisé en ce que ladite amorce et/ou ladite sonde sont choisies parmi les séquences nucléotidiques selon l'une des revendications 2 à 4, 8 à 26, 51 à 55 et 77 spécifiques de l'espèce Listeria monocytogenes, en ce que lesdits polypeptides sont choisis parmi les polypeptides selon l'une des revendications 5 à 7, 27 à 44 et 74 ou 76 spécifiques de l'espèce Listeria monocytogenes et en ce que lesdits anticorps sont choisis parmi les anticorps selon l'une des revendications 79 et 80 dirigés contre les polypeptides choisis parmi les polypeptides selon l'une des revendications 5 à 7, 26 à 44, 74 ou 76 spécifiques de l'espèce Listeria monocytogenes.
98. Procédé ou kit ou nécessaire selon la revendication 97, caractérisé en ce que ladite amorce et/ou ladite sonde sont choisies parmi les séquences nucléotidiques codant pour une protéine sécrétée, en ce que lesdits polypeptides sont choisis parmi les polypeptides sécrétés et en ce que lesdits anticorps sont choisis parmi les anticorps selon l'une des revendications 79 et 80 dirigés contre des polypeptides sécrétés ou de surface de Listeria monocytogenes, lesdits polypeptide étant de préférence SEQ ID N° 2 à SEQ ID N°41 .
99. Souche de Listeria monocytogenes, caractérisée en ce qu'elle contient au moins une mutation dans au moins une séquence nucléotidique selon l'une des revendications 2 à 4 ou 8 à 26.
100. Souche de Listeria monocytogenes selon la revendication 99, caractérisée en ce qu'elle contient une mutation dans une séquence nucléotidique selon la revendication 13.
101. Souche de Listeria monocytogenes selon la revendication 99, caractérisée en ce qu'elle contient une mutation dans une séquence nucléotidique selon la revendication 14.
102. Souche de Listeria monocytogenes selon la revendication 99, caractérisée en ce qu'elle contient une mutation dans une séquence nucléotidique selon la revendication 9, 19 ou 20.
103. Souche de Listeria monocytogenes selon la revendication 99, caractérisée en ce qu'elle contient une mutation dans une séquence nucléotidique selon la revendication 12, en particulier codant pour une protéine impliquée dans les mécanismes de sécrétion.
104. Souche de Listeria monocytogenes selon la revendication 99, caractérisée en ce qu'elle contient une mutation dans une séquence nucléotidique codant pour une protéine impliquée dans la résistance et/ou l'adaptation au stress.
105. Souche de Listeria monocytogenes selon la revendication 99, caractérisée en ce qu'elle contient une mutation dans une séquence nucléotidique codant pour une protéine impliquée dans la biosynthèse de la vitamine B 12.
106. Souche de Listeria monocytogenes selon l'une des revendications 99 à 105, caractérisée en ce que la mutation mène à une inactivation du gène.
107. Souche de Listeria monocytogenes selon l'une des revendications 99 à 105, caractérisée en ce que la mutation mène à une surexpression du gène.
108. Utilisation d'un polypeptide selon l'une des revendications 5 à 7, 27 à 44, 74 et 76 d'une cellule transformée selon l'une des revendications 69 à 71 d'une souche selon la revendication 99 à 107 et/ou d'un animal selon la revendication 72, pour la biosynthèse ou la biodégradation d'uri composé d'intérêt.
109. Procédé de biosynthèse ou de biodégradation d'un composé d'intérêt, caractérisé en ce qu'il met en œuvre un polypeptide selon l'une des revendications 5 à 7, 26 à 44, 74 et 76, une cellule transformée selon l'une des revendications 69 à 71 une souche selon l'une des revendications 99 h 107 et/ou un animal selon la revendication 72.
110. Utilisation selon la revendication 108 ou procédé selon la revendication 109, caractérisés en ce que le composé d'intérêt est la vitamine B12.
111. Utilisation d'une séquence nucléotidique selon l'une des revendications 2 à 4, et, 8 à 26, d'un polypeptide selon l'une des revendications 5 à 7, 27 à 44, 74 et 76, d'un anticorps selon l'une des revendications 79 et 80, d'une cellule selon l'une des revendications 69 à 71, et/ou d'un animal transformé selon la revendication 72 pour la sélection de composé organique ou inorganique capable de moduler, de réguler, d'induire ou d'inhiber l'expression de gènes, et/ou de modifier la réplication cellulaire de cellules eucaryotes ou procaryotes ou capables d'induire, d'inhiber ou d'aggraver chez un organisme animal ou humain les pathologies liées à une infection par Listeria monocytogenes ou par un micro-organisme associé.
112. Méthode de sélection de composé capable de se lier à un polypeptide selon l'une des revendications 5 à 7, 27 à 44, 74 et 76, capable de se lier à une séquence nucléotidique selon l'une des revendications 2 à 4, et, 8 à 26, ou capable de reconnaître un anticorps selon l'une des revendications 79 et 80, et/ou capable de moduler, de réguler, d'induire ou d'inhiber l'expression de gènes, et/ou de modifier la réplication cellulaire de cellules eucaryotes ou procaryotes, ou capables d'induire, d'inhiber ou d'aggraver chez un organisme animal ou humain les pathologies liées à une infection par Listeria monocytogenes, caractérisée en ce qu'elle comprend les étapes suivantes : a) mise en contact dudit composé avec ledit polypeptide, ladite séquence nucléotidique, avec une cellule transformée selon l'une des revendications 69 à 71, et/ou administration dudit composé à un animal transformé selon la revendication 72; b) détermination de la capacité dudit composé à se lier avec ledit polypeptide ou ladite séquence nucléotidique, ou de moduler, de réguler, d'induire ou d'inhiber l'expression de gènes, ou de moduler la croissance ou la réplication cellulaire, ou d'induire, d'inhiber ou d'aggraver chez ledit organisme animal ou humain les pathologies liées à une infection par Listeria monocytogenes ou par un micro-organisme associé.
113. Composé susceptible d'être sélectionné par une méthode selon la revendication 112.
114. Composition pharmaceutique comprenant un composé choisi parmi les composés suivants : a) une séquence nucléotidique selon l'une des revendications 2 à 4, et 8 à 26 ; b) un polypeptide selon l'une des revendications 5 à 7, 27 à 44, 74 et 76 ; c) un vecteur selon l'une des revendications 62 à 68, et 78 ; d) un anticorps selon la revendication 79 ou 80 ; et e) un composé selon la revendication 113.
115. Composition selon la revendication 114, éventuellement en association avec un véhicule pharmaceutiquement acceptable.
116. Composition pharmaceutique selon l'une des revendications 114 et 115 pour la prévention ou le traitement d'une infection par une bactérie appartenant à l'espèce Listeria monocytogenes ou par un micro-organisme associé.
117. Composition vaccinale, caractérisée en ce qu'elle comprend un ou plusieurs polypeptides selon l'une des revendications 5 à 7, 27 à 44, 74, et/ou un ou plusieurs polypeptides hybrides selon la revendication 76.
118. Utilisation d'une cellule selon l'une des revendications 62 à 68, pour la préparation d'une composition vaccinale.
1 19. Composition vaccinale, caractérisée en ce qu'elle contient un vecteur selon l'une des revendications 62 à 68 et 78, et/ou une cellule selon l'une des revendications 69 à 71.
120. Composition vaccinale capable d'induire une réponse immunitaire cellulaire ou humorale pour la prévention ou le traitement d'une infection par bactérie appartenant à l'espèce Listeria monocytogenes ou par un micro-organisme associé, caractérisée en ce qu'elle comprend une composition immunogène selon l'une des revendications 117 et 119, en association avec un véhicule pharmaceutiquement acceptable et, éventuellement un ou plusieurs adjuvants de l'immunité appropriés.
121. Banque d'ADN génomique d'une bactérie du genre Listeria, caractérisée en ce que ladite banque d'ADN est clonée dans des chromosomes artificiels de bactéries (BAC).
122. Banque d'ADN génomique selon la revendication 121, caractérisée en ce que ladite bactérie est Listeria monocytogenes.
123. Banque d'ADN génomique selon la revendication 121, caractérisée en ce que ladite bactérie est la souche EGD-e de Listeria monocytogenes.
124. Banque selon la revendication 122, caractérisée en ce qu'il s'agit de la banque LM-baclib déposée à la CNCM le 11 Avril 2000, sous le numéro d'ordre 1-2439.
125. Méthode pour l'isolement d'un polynucléotide d'intérêt présent chez une souche de Listeria et absente chez une autre souche, caractérisé en ce quelle utilise au moins une banque selon l'une des revendications 121 ou 124.
126. Méthode selon la revendication 125, caractérisée en ce qu'elle comprend les étapes suivantes : a) isoler au moins un polynucléotide contenu dans un clone de la banque d'ADN basée sur un BAC, d'origine de Listeria, b) isoler :
- au moins un polynucléotide génomique ou ADNc d'une Listeria, ladite Listeria appartenant à une souche différente de la souche utilisée pour la construction de la banque d'ADN BAC de l'étape a) ou, de façon alternative,
- au moins un polynucléotide contenu dans un clone d'une banque d'ADN basée sur un BAC préparé à partir du génome d'une Listeria qui est différente de la Listeria utilisée pour la construction de la banque d'ADN basée sur le BAC de l'étape a). c) hybrider le polynucléotide de l'étape a) au polynucléotide de l'étape b) ; d) sélectionner les polynucléotides de l'étape a) qui n'ont pas formé de complexe d'hybridation avec les polynucléotides de l'étape b) ; e) caractériser le polynucléotide sélectionné.
127. Banque génomique d'une bactérie du genre Listeria.
128. Banque génomique selon la revendication 127, caractérisée en ce que la bactérie est Listeria monocytogenes.
PCT/FR2001/001118 2000-04-11 2001-04-11 Genome de listeria monocytogenes, polypeptides et utilisations Ceased WO2001077335A2 (fr)

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WO2003076463A3 (fr) * 2002-03-11 2003-12-11 Wageningen Ct For Food Science BACTERIES GRAM POSITIVES PLUS RESISTANTES AU STRESS, A FAIBLE VIRULENCE, ET A GENE ctsR MODIFIE
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US7052896B2 (en) 2000-08-08 2006-05-30 Matthew Glenn Lactobacillus rhamnosus polynucleotides, polypeptides and methods for using them
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