WO2001098528A3 - Verfahren zum nachweis von cytosin-methylierungen - Google Patents

Verfahren zum nachweis von cytosin-methylierungen Download PDF

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Publication number
WO2001098528A3
WO2001098528A3 PCT/DE2001/002274 DE0102274W WO0198528A3 WO 2001098528 A3 WO2001098528 A3 WO 2001098528A3 DE 0102274 W DE0102274 W DE 0102274W WO 0198528 A3 WO0198528 A3 WO 0198528A3
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WO
WIPO (PCT)
Prior art keywords
genomic dna
identifying mark
methylcytosine
dna
pcr products
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2001/002274
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English (en)
French (fr)
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WO2001098528A2 (de
Inventor
Kurt Berlin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
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Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE50110885T priority Critical patent/DE50110885D1/de
Priority to CA2407876A priority patent/CA2407876C/en
Priority to US10/311,661 priority patent/US7118868B2/en
Priority to EP01953113A priority patent/EP1294945B1/de
Priority to AU2001275650A priority patent/AU2001275650B2/en
Priority to IL15256901A priority patent/IL152569A0/xx
Priority to AU7565001A priority patent/AU7565001A/xx
Priority to NZ522531A priority patent/NZ522531A/en
Application filed by Epigenomics AG filed Critical Epigenomics AG
Priority to JP2002504676A priority patent/JP4489347B2/ja
Publication of WO2001098528A2 publication Critical patent/WO2001098528A2/de
Publication of WO2001098528A3 publication Critical patent/WO2001098528A3/de
Anticipated expiration legal-status Critical
Priority to US10/964,167 priority patent/US20060134643A1/en
Priority to US11/544,135 priority patent/US8241855B2/en
Priority to US12/410,389 priority patent/US7968295B2/en
Priority to US13/159,323 priority patent/US8257950B2/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/101—Primer extension

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Beschrieben wird ein Verfahren zum Nachweis von 5-Methylcytosin in genomischen DNA-Proben. Zuerst wird eine genomische DNA aus einer DNA-Probe mit einem Reagenz chemisch umgesetzt, wobei 5-Methylcytosin und Cytosin unterschiedlich reagieren. Anschliessend wird die vorbehandelte DNA unter Verwendung einer Polymerase mit Primern unterschiedlicher Sequenz amplifiziert. Im nächsten Schritt wird die amplifizierte genomische DNA auf einen Oligonukleotid Array hybridisiert und PCR-Produkte erhalten, die mit einer Markierung versehen sein müssen. Alternativ können die PCR-Produkte in einer Primer Extention Reaktion verlängert werden, wobei auch die Verlängerungsprodukte mit einer Markierung versehen sind. Im letzten Schritt werden die verlängerten Oligonukleotide auf das Vorhandensein der Markierung untersucht.
PCT/DE2001/002274 2000-06-19 2001-06-19 Verfahren zum nachweis von cytosin-methylierungen Ceased WO2001098528A2 (de)

Priority Applications (13)

Application Number Priority Date Filing Date Title
NZ522531A NZ522531A (en) 2000-06-19 2001-06-19 Method for detecting cytosine methylations
CA2407876A CA2407876C (en) 2000-06-19 2001-06-19 Method for the detection of cytosine methylations
US10/311,661 US7118868B2 (en) 2000-06-19 2001-06-19 Method for detecting cytosine methylations
EP01953113A EP1294945B1 (de) 2000-06-19 2001-06-19 Verfahren zum nachweis von cytosin-methylierungen
AU2001275650A AU2001275650B2 (en) 2000-06-19 2001-06-19 Method for detecting cytosine methylations
IL15256901A IL152569A0 (en) 2000-06-19 2001-06-19 Method for detecting cytosine methylations
AU7565001A AU7565001A (en) 2000-06-19 2001-06-19 Method for detecting cytosine methylations
DE50110885T DE50110885D1 (de) 2000-06-19 2001-06-19 Verfahren zum nachweis von cytosin-methylierungen
JP2002504676A JP4489347B2 (ja) 2000-06-19 2001-06-19 シトシン−メチル化の検出方法
US10/964,167 US20060134643A1 (en) 2000-06-19 2004-10-12 Bisulfite conversion of DNA
US11/544,135 US8241855B2 (en) 2000-06-19 2006-10-07 Method for detecting cytosine methylations
US12/410,389 US7968295B2 (en) 2000-06-19 2009-03-24 Bisulfite conversion of DNA
US13/159,323 US8257950B2 (en) 2000-06-19 2011-06-13 Bisulfite conversion of DNA

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10029915.6 2000-06-19
DE10029915A DE10029915B4 (de) 2000-06-19 2000-06-19 Verfahren zum Nachweis von Cytosin-Methylierungen

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US10311661 A-371-Of-International 2001-06-19
US10/964,167 Continuation-In-Part US20060134643A1 (en) 2000-06-19 2004-10-12 Bisulfite conversion of DNA
US11/544,135 Division US8241855B2 (en) 2000-06-19 2006-10-07 Method for detecting cytosine methylations

Publications (2)

Publication Number Publication Date
WO2001098528A2 WO2001098528A2 (de) 2001-12-27
WO2001098528A3 true WO2001098528A3 (de) 2002-11-28

Family

ID=7646113

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/002274 Ceased WO2001098528A2 (de) 2000-06-19 2001-06-19 Verfahren zum nachweis von cytosin-methylierungen

Country Status (12)

Country Link
US (2) US7118868B2 (de)
EP (2) EP1707642A3 (de)
JP (1) JP4489347B2 (de)
AT (1) ATE338143T1 (de)
AU (2) AU7565001A (de)
CA (1) CA2407876C (de)
DE (2) DE10029915B4 (de)
DK (1) DK1294945T3 (de)
ES (1) ES2272504T3 (de)
IL (1) IL152569A0 (de)
NZ (1) NZ522531A (de)
WO (1) WO2001098528A2 (de)

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DE10010281B4 (de) * 2000-02-25 2005-03-10 Epigenomics Ag Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
US20060134643A1 (en) 2000-06-19 2006-06-22 Kurt Berlin Bisulfite conversion of DNA
DE10347396B4 (de) * 2003-10-09 2005-06-23 Epigenomics Ag Optimierte Bisulfit-Umwandlung von DNA durch Einsatz von Dioxan
DE10347397B4 (de) * 2003-10-09 2005-08-04 Epigenomics Ag Optimierte Bisulfit-Umwandlung durch Zusatz von n-Alkylenglykol-Verbindungen
DE10029915B4 (de) * 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
DE10347400B4 (de) * 2003-10-09 2005-08-04 Epigenomics Ag Verbesserte Bisulfit-Umwandlung von DNA durch kurzzeitige Temperaturerhöhungen
DE10347399B4 (de) * 2003-10-09 2005-09-15 Epigenomics Ag Bisulfit-Umwandlung zum Nachweis von Cytosin-Methylierungen in DNA mittels optimierter Aufreinigung
DE10154317B4 (de) * 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
DE10201138B4 (de) * 2002-01-08 2005-03-10 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA)
EP1340818A1 (de) * 2002-02-27 2003-09-03 Epigenomics AG Verfahren und Nukleinsäuren zur Analyse von Kolonkrebszellen
DE602004009038T3 (de) 2003-01-29 2015-06-25 Roche Diagnostics Gmbh Verbessertes Verfahren zur Behandlung durch Bisulfit
DE10338308B4 (de) 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
US7262013B2 (en) * 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
US7371526B2 (en) 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
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CA2540310A1 (en) * 2003-10-09 2005-04-28 Epigenomics Ag Improved bisulfite conversion of dna
WO2005054502A1 (en) 2003-12-02 2005-06-16 Roche Diagnostics Gmbh Improved method for bisulfite treatment
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US7399614B2 (en) * 2004-06-30 2008-07-15 Applera Corporation 5-methylcytosine detection, compositions and methods therefor
EP1632578A1 (de) 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
ES2787454T3 (es) 2004-09-30 2020-10-16 Epigenomics Ag Método para proveer fragmentos de ADN derivados de una muestra archivada
US7658288B2 (en) * 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
EP2803734B1 (de) 2005-04-01 2017-09-20 Epigenomics AG Verbesserte Bisulfit-Umwandlung von DNA
WO2006113770A1 (en) 2005-04-15 2006-10-26 Epigenomics Ag A method for providing dna fragments derived from a remote sample
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DE502005008746D1 (de) 2005-07-21 2010-02-04 Epigenomics Ag Verfahren zur Quantifizierung methylierter DNA
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EP2071035A1 (de) 2007-12-13 2009-06-17 Epigenomics AG Vermittler und Verstärkungsverfahren
EP2189538A1 (de) 2008-11-21 2010-05-26 Epigenomics AG Analysemolekül und Verfahren zur Analyse einer Nucleotid-Position in einer Nukleinsäure
WO2013148147A1 (en) 2012-03-26 2013-10-03 The U.S.A., As Represented By The Secretary Dept. Of Health And Human Services Dna methylation analysis for the diagnosis, prognosis and treatment of adrenal neoplasms
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US10913986B2 (en) 2016-02-01 2021-02-09 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
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US11725190B2 (en) 2019-02-22 2023-08-15 EMULATE, Inc. Microfluidic proximal tubule kidney-on-chip
DE102019118332B4 (de) 2019-07-07 2022-04-07 Ist Innuscreen Gmbh Verfahren und testkit zur bisulfitmodifizierung von dna
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9394332B2 (en) 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment

Also Published As

Publication number Publication date
AU7565001A (en) 2002-01-02
WO2001098528A2 (de) 2001-12-27
IL152569A0 (en) 2003-05-29
CA2407876A1 (en) 2001-12-27
JP2004500892A (ja) 2004-01-15
EP1707642A2 (de) 2006-10-04
EP1294945B1 (de) 2006-08-30
US8241855B2 (en) 2012-08-14
EP1707642A3 (de) 2006-12-27
EP1294945A2 (de) 2003-03-26
CA2407876C (en) 2012-03-20
JP4489347B2 (ja) 2010-06-23
DK1294945T3 (da) 2007-01-08
NZ522531A (en) 2005-01-28
AU2001275650B2 (en) 2005-11-03
DE10029915B4 (de) 2005-06-16
ES2272504T3 (es) 2007-05-01
DE50110885D1 (de) 2006-10-12
US7118868B2 (en) 2006-10-10
US20040152080A1 (en) 2004-08-05
ATE338143T1 (de) 2006-09-15
US20070207472A1 (en) 2007-09-06
DE10029915A1 (de) 2002-01-03

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