WO2002000832A2 - Nouveau polypeptide, proteine p80-coiline 8.8, et polynucleotide codant ce polypeptide - Google Patents

Nouveau polypeptide, proteine p80-coiline 8.8, et polynucleotide codant ce polypeptide Download PDF

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Publication number
WO2002000832A2
WO2002000832A2 PCT/CN2001/000954 CN0100954W WO0200832A2 WO 2002000832 A2 WO2002000832 A2 WO 2002000832A2 CN 0100954 W CN0100954 W CN 0100954W WO 0200832 A2 WO0200832 A2 WO 0200832A2
Authority
WO
WIPO (PCT)
Prior art keywords
protein
polypeptide
polynucleotide
coilin
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2001/000954
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English (en)
Chinese (zh)
Other versions
WO2002000832A3 (fr
Inventor
Yumin Mao
Yi Xie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Biowindow Gene Development Inc
Original Assignee
Shanghai Biowindow Gene Development Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Biowindow Gene Development Inc filed Critical Shanghai Biowindow Gene Development Inc
Priority to AU89538/01A priority Critical patent/AU8953801A/en
Publication of WO2002000832A2 publication Critical patent/WO2002000832A2/fr
Publication of WO2002000832A3 publication Critical patent/WO2002000832A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals

Definitions

  • isolated protein p80-coilin 8.8 refers to a P 80 - coi 1 in 8.8 protein substantially free of naturally associated with other proteins, lipids, carbohydrates or other substances.
  • Those skilled in the art can purify p80-coilin protein 8.8 using standard protein purification techniques. Substantially pure polypeptides can produce a single main band on a non-reducing polyacrylamide gel. The purity of p80-coilin 8.8 peptide can be analyzed by amino acid sequence.
  • the protein product of p80- CO il in protein 8.8 gene expression can be detected by immunological techniques such as Western blotting, radioimmunoprecipitation, and enzyme-linked immunosorbent assay (ELISA).
  • a method for amplifying DNA / RNA using PCR technology is preferably used to obtain the gene of the present invention.
  • the RACE method RACE-Rapid Amplification of cDNA Ends
  • the primers used for PCR can be appropriately based on the polynucleotide sequence information of the present invention disclosed herein. Selected and synthesized by conventional methods.
  • the amplified DNA / RNA fragments can be isolated and purified by conventional methods such as by gel electrophoresis.
  • the invention also relates to a diagnostic test method for quantitatively and locally detecting the level of p80-coiiin protein 8.8.
  • tests are well known in the art and include FISH assays and radioimmunoassays.
  • the level of p80-coiiin protein 8.8 detected in the test can be used to explain the importance of p80-coi 1 in protein 8.8 in various diseases and to diagnose diseases in which p80-coiiin protein 8.8 plays a role.
  • polypeptide of the present invention can also be used for peptide mapping analysis.
  • the polypeptide can be specifically cleaved by physical, chemical or enzymatic analysis, and subjected to one-dimensional or two-dimensional or three-dimensional gel electrophoresis analysis, preferably mass spectrometry.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne un nouveau polypeptide, une protéine p80-coiline 8.8, et un polynucléotide codant ce polypeptide ainsi qu'un procédé d'obtention de ce polypeptide par des techniques recombinantes d'ADN. L'invention concerne en outre les applications de ce polypeptide dans le traitement de maladies, notamment des tumeurs malignes, de l'hémopathie, de troubles du développement, de l'infection par VIH, de maladies immunitaires et de diverses inflammations. L'invention concerne aussi l'antagoniste agissant contre le polypeptide et son action thérapeutique ainsi que les applications de ce polynucléotide codant la protéine p80-coiline 8.8.
PCT/CN2001/000954 2000-06-14 2001-06-11 Nouveau polypeptide, proteine p80-coiline 8.8, et polynucleotide codant ce polypeptide Ceased WO2002000832A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU89538/01A AU8953801A (en) 2000-06-14 2001-06-11 A novel polypeptide, a p80-coiline protein 8.8 and the polynucleotide encoding the polypeptide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN00116512.7 2000-06-14
CN 00116512 CN1328012A (zh) 2000-06-14 2000-06-14 一种新的多肽——p80－coilin蛋白8.8和编码这种多肽的多核苷酸

Publications (2)

Publication Number Publication Date
WO2002000832A2 true WO2002000832A2 (fr) 2002-01-03
WO2002000832A3 WO2002000832A3 (fr) 2002-04-18

Family

ID=4585916

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2001/000954 Ceased WO2002000832A2 (fr) 2000-06-14 2001-06-11 Nouveau polypeptide, proteine p80-coiline 8.8, et polynucleotide codant ce polypeptide

Country Status (3)

Country Link
CN (1) CN1328012A (fr)
AU (1) AU8953801A (fr)
WO (1) WO2002000832A2 (fr)

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [Online] 12 March 2000 LAMERDIN J.E. ET AL. Retrieved from NCBI, accession no. GI:7229304 Database accession no. (AC025588.1) *
DATABASE PROTEIN [Online] 28 May 1998 HAN Z.S. ET AL. Retrieved from NCBI, accession no. GI:3158392 Database accession no. (AAC39036.1) *

Also Published As

Publication number Publication date
WO2002000832A3 (fr) 2002-04-18
CN1328012A (zh) 2001-12-26
AU8953801A (en) 2002-01-08

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