WO2002000902A3 - Regulation of anthocyanin pigment production - Google Patents

Regulation of anthocyanin pigment production Download PDF

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Publication number
WO2002000902A3
WO2002000902A3 PCT/US2001/019734 US0119734W WO0200902A3 WO 2002000902 A3 WO2002000902 A3 WO 2002000902A3 US 0119734 W US0119734 W US 0119734W WO 0200902 A3 WO0200902 A3 WO 0200902A3
Authority
WO
WIPO (PCT)
Prior art keywords
regulation
pigment production
pap1
anthocyanin pigment
pap2
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/019734
Other languages
French (fr)
Other versions
WO2002000902A2 (en
Inventor
Justin Borevitz
Yiji Xia
Christopher J Lamb
Richard A Dixon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roberts Samuels Noble Foundation Inc
Salk Institute for Biological Studies
Original Assignee
Roberts Samuels Noble Foundation Inc
Salk Institute for Biological Studies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/610,185 external-priority patent/US6573432B1/en
Application filed by Roberts Samuels Noble Foundation Inc, Salk Institute for Biological Studies filed Critical Roberts Samuels Noble Foundation Inc
Priority to AU2001269960A priority Critical patent/AU2001269960A1/en
Publication of WO2002000902A2 publication Critical patent/WO2002000902A2/en
Publication of WO2002000902A3 publication Critical patent/WO2002000902A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method for determining gene expression in transgenic plants is disclosed. This method includes providing expression vectors having the PAP1 or PAP2 gene linked to an expressed gene of interest. If the expression vector is activated, the PAP1 or PAP2 genes confer a purple pigmentation to the transgenic plant. Thus, plants that have been successfully transformed are easily identifiable by visual inspection.
PCT/US2001/019734 2000-06-23 2001-06-21 Regulation of anthocyanin pigment production Ceased WO2002000902A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001269960A AU2001269960A1 (en) 2000-06-23 2001-06-21 Regulation of anthocyanin pigment production

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US60324400A 2000-06-23 2000-06-23
US09/603,244 2000-06-23
US09/610,185 US6573432B1 (en) 2000-07-05 2000-07-05 Regulation of anthocyanin pigment production
US09/610,185 2000-07-05

Publications (2)

Publication Number Publication Date
WO2002000902A2 WO2002000902A2 (en) 2002-01-03
WO2002000902A3 true WO2002000902A3 (en) 2002-10-03

Family

ID=27084366

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/019734 Ceased WO2002000902A2 (en) 2000-06-23 2001-06-21 Regulation of anthocyanin pigment production

Country Status (2)

Country Link
AU (1) AU2001269960A1 (en)
WO (1) WO2002000902A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8008543B2 (en) 2005-10-26 2011-08-30 Agriculture Victoria Services Pty Ltd Modification of flavonoid biosynthesis in plants by PAP1
AU2006308510B2 (en) * 2005-10-26 2013-01-10 Agriculture Victoria Services Pty Ltd Modification of flavonoid biosynthesis in plants
WO2009063460A2 (en) * 2007-11-13 2009-05-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Methods of modulating production of phenylpropanoid compounds in plants
WO2014074444A1 (en) * 2012-11-06 2014-05-15 Ohio State Innovation Foundation Methods for inducing anthocyanin production in plants
RU2017105124A (en) 2014-07-30 2018-08-28 Сикпа Холдинг Са METHODS FOR PRODUCING LAYERS WITH OPTICAL EFFECT USING A BELT DRIVE
CN110628781B (en) * 2019-10-17 2021-04-27 中国林业科学研究院林业研究所 An R2R3 MYB transcription factor that promotes anthocyanin formation in orchids
CN111875686B (en) * 2020-07-08 2021-11-23 中国农业科学院油料作物研究所 Application of sesamin SiLLR in regulation and control of plant root system development
CN111875687B (en) * 2020-07-08 2021-11-23 中国农业科学院油料作物研究所 Application of sesame protein SiBRB in regulation and control of plant root system development
CN120425081B (en) * 2025-05-19 2026-03-20 河南省农业科学院蔬菜研究所 InDel marker combinatorial analysis of genes related to purple stigma in Chinese cabbage and their application

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998013486A1 (en) * 1996-09-27 1998-04-02 Rutgers, The State University Of New Jersey Novel genes associated with enhanced disease resistance in plants
WO2001032002A1 (en) * 1999-11-05 2001-05-10 Basf Corporation Myb transcription factors and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998013486A1 (en) * 1996-09-27 1998-04-02 Rutgers, The State University Of New Jersey Novel genes associated with enhanced disease resistance in plants
WO2001032002A1 (en) * 1999-11-05 2001-05-10 Basf Corporation Myb transcription factors and uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ABE H ET AL: "ROLE OF ARABIDOPSIS MYC AND MYB HOMOLOGS IN DROUGHT- AND ABSCISIC ACID-REGULATED GENE EXPRESSION", PLANT CELL, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, US, vol. 9, October 1997 (1997-10-01), pages 1859 - 1868, XP002937955, ISSN: 1040-4651 *
BOREVITZ JUSTIN O ET AL: "Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis.", PLANT CELL, vol. 12, no. 12, December 2000 (2000-12-01), pages 2383 - 2393, XP002196250, ISSN: 1040-4651 *
DATABASE EM_PL EBI Hinxton, UK; 2 December 1998 (1998-12-02), KRANZ H D ET AL, XP002196251 *
KIRIK V ET AL: "ECTOPIC EXPRESSION OF A NOVEL MYB GENE MODIFIES THE ARCHITECTURE OF THE ARABIDOPSIS INFLORESCENCE", PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 13, no. 6, 1998, pages 729 - 742, XP000929257, ISSN: 0960-7412 *
KRANZ H D ET AL: "TOWARDS FUNCTIONAL CHARACTERISATION OF THE MEMBERS OF THE R2R3-MYB GENE FAMILY FROM ARABIDOPSIS THALIANA", PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 16, no. 2, 1998, pages 263 - 276, XP000929447, ISSN: 0960-7412 *

Also Published As

Publication number Publication date
WO2002000902A2 (en) 2002-01-03
AU2001269960A1 (en) 2002-01-08

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