WO2002014476A2 - Methodes et trousses destinees a identifier et quantifier des arn et des adn associes aux proteines de liaison a l'arn et a l'adn - Google Patents

Methodes et trousses destinees a identifier et quantifier des arn et des adn associes aux proteines de liaison a l'arn et a l'adn Download PDF

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Publication number
WO2002014476A2
WO2002014476A2 PCT/US2001/025804 US0125804W WO0214476A2 WO 2002014476 A2 WO2002014476 A2 WO 2002014476A2 US 0125804 W US0125804 W US 0125804W WO 0214476 A2 WO0214476 A2 WO 0214476A2
Authority
WO
WIPO (PCT)
Prior art keywords
detector
rna
rnas
bound
dnas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/025804
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English (en)
Other versions
WO2002014476A3 (fr
Inventor
James H. Eberwine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania Penn
Original Assignee
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn
Priority to AU2001288289A priority Critical patent/AU2001288289A1/en
Priority to US10/344,467 priority patent/US7122313B2/en
Publication of WO2002014476A2 publication Critical patent/WO2002014476A2/fr
Publication of WO2002014476A3 publication Critical patent/WO2002014476A3/fr
Anticipated expiration legal-status Critical
Priority to US11/518,719 priority patent/US20070003973A1/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes et des trousses destinées à identifier des ARN et des ADN associés à une protéine de liaison à l'ARN ou à l'ADN par l'intermédiaire d'un détecteur spécifique à cette protéine de liaison ou à une protéine qui lui est associée, ce détecteur étant lié à un oligonucléotide capable de diriger la synthèse de copies d'ADN complémentaire d'ARN ou d'ADN se trouvant au voisinage ou à proximité immédiate du détecteur lié.
PCT/US2001/025804 2000-08-17 2001-08-17 Methodes et trousses destinees a identifier et quantifier des arn et des adn associes aux proteines de liaison a l'arn et a l'adn Ceased WO2002014476A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2001288289A AU2001288289A1 (en) 2000-08-17 2001-08-17 Methods and kits for identifying and quantifying rnas and dnas associated with rna and dna binding proteins
US10/344,467 US7122313B2 (en) 2001-08-17 2001-08-17 Methods and kits for identifying and quantifying RNAs and DNAs associated with RNA and DNA binding proteins
US11/518,719 US20070003973A1 (en) 2000-08-17 2006-09-11 Methods and kits for identifying and quantifying RNAs and DNAs associated with RNA and DNA binding proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22585800P 2000-08-17 2000-08-17
US60/225,858 2000-08-17

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/518,719 Continuation US20070003973A1 (en) 2000-08-17 2006-09-11 Methods and kits for identifying and quantifying RNAs and DNAs associated with RNA and DNA binding proteins

Publications (2)

Publication Number Publication Date
WO2002014476A2 true WO2002014476A2 (fr) 2002-02-21
WO2002014476A3 WO2002014476A3 (fr) 2002-05-30

Family

ID=22846535

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/025804 Ceased WO2002014476A2 (fr) 2000-08-17 2001-08-17 Methodes et trousses destinees a identifier et quantifier des arn et des adn associes aux proteines de liaison a l'arn et a l'adn

Country Status (2)

Country Link
AU (1) AU2001288289A1 (fr)
WO (1) WO2002014476A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004048596A2 (fr) 2002-11-21 2004-06-10 Epicentre Technologies Procedes d'utilisation d'amorces qui codent un brin d'un promoteur bicatenaire
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US5922617A (en) * 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices
US6087112A (en) * 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
WO2004048596A2 (fr) 2002-11-21 2004-06-10 Epicentre Technologies Procedes d'utilisation d'amorces qui codent un brin d'un promoteur bicatenaire

Also Published As

Publication number Publication date
AU2001288289A1 (en) 2002-02-25
WO2002014476A3 (fr) 2002-05-30

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