WO2002018643A3 - Nanoparticles having oligonucleotides attached thereto and uses therefor - Google Patents

Nanoparticles having oligonucleotides attached thereto and uses therefor Download PDF

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Publication number
WO2002018643A3
WO2002018643A3 PCT/US2001/025237 US0125237W WO0218643A3 WO 2002018643 A3 WO2002018643 A3 WO 2002018643A3 US 0125237 W US0125237 W US 0125237W WO 0218643 A3 WO0218643 A3 WO 0218643A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
methods
nucleic acid
provides
oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/025237
Other languages
French (fr)
Other versions
WO2002018643A2 (en
WO2002018643A9 (en
Inventor
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Thomas Andrew Taton
Viswanadham Garimella
Zhi Li
So-Jung Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanosphere LLC
Original Assignee
Nanosphere LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/760,500 external-priority patent/US6767702B2/en
Priority claimed from US09/820,279 external-priority patent/US6750016B2/en
Priority to CA002418502A priority Critical patent/CA2418502A1/en
Application filed by Nanosphere LLC filed Critical Nanosphere LLC
Priority to EP01959723A priority patent/EP1362121A2/en
Priority to AU2001281248A priority patent/AU2001281248A1/en
Priority to JP2002522548A priority patent/JP2004525607A/en
Publication of WO2002018643A2 publication Critical patent/WO2002018643A2/en
Anticipated expiration legal-status Critical
Publication of WO2002018643A3 publication Critical patent/WO2002018643A3/en
Publication of WO2002018643A9 publication Critical patent/WO2002018643A9/en
Priority to AU2006200274A priority patent/AU2006200274A1/en
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Nanotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comrising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
PCT/US2001/025237 2000-08-11 2001-08-10 Nanoparticles having oligonucleotides attached thereto and uses therefor Ceased WO2002018643A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2002522548A JP2004525607A (en) 2000-08-11 2001-08-10 Oligonucleotide-attached nanoparticles and methods of use
AU2001281248A AU2001281248A1 (en) 2000-08-11 2001-08-10 Nanoparticles having oligonucleotides attached thereto and uses therefor
CA002418502A CA2418502A1 (en) 2000-08-11 2001-08-10 Nanoparticles having oligonucleotides attached thereto and uses therefor
EP01959723A EP1362121A2 (en) 2000-08-11 2001-08-10 Nanoparticles having oligonucleotides attached thereto and uses therefor
AU2006200274A AU2006200274A1 (en) 2000-08-11 2006-01-23 Nanoparticles Having Oligonucleotides Attached thereto and uses therefor

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US22463100P 2000-08-11 2000-08-11
US60/224,631 2000-08-11
US25439200P 2000-12-08 2000-12-08
US60/254,392 2000-12-08
US25523500P 2000-12-11 2000-12-11
US60/255,235 2000-12-11
US09/760,500 US6767702B2 (en) 1996-07-29 2001-01-12 Nanoparticles having oligonucleotides attached thereto and uses therefor
US09/760,500 2001-01-12
US09/820,279 2001-03-28
US09/820,279 US6750016B2 (en) 1996-07-29 2001-03-28 Nanoparticles having oligonucleotides attached thereto and uses therefor

Publications (3)

Publication Number Publication Date
WO2002018643A2 WO2002018643A2 (en) 2002-03-07
WO2002018643A3 true WO2002018643A3 (en) 2003-09-04
WO2002018643A9 WO2002018643A9 (en) 2003-11-06

Family

ID=27539880

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/025237 Ceased WO2002018643A2 (en) 2000-08-11 2001-08-10 Nanoparticles having oligonucleotides attached thereto and uses therefor

Country Status (5)

Country Link
EP (1) EP1362121A2 (en)
JP (1) JP2004525607A (en)
AU (1) AU2001281248A1 (en)
CA (1) CA2418502A1 (en)
WO (1) WO2002018643A2 (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US7491496B2 (en) 2004-04-01 2009-02-17 Seiko Epson Corporation Method for immobilizing nucleic acid and method for manufacturing biosensor using the same method
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US11618790B2 (en) 2010-12-23 2023-04-04 Hoffmann-La Roche Inc. Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69737598T2 (en) 1996-07-29 2007-12-27 Nanosphere Inc., Skokie NANOPARTICLES WITH OLIGONUCLEOTIDES ADDITED TO IT AND THEIR USES
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
DE60119170T2 (en) 2000-07-11 2007-05-24 Northwestern University, Evanston DETECTION METHOD OF REINFORCED SILVER DYEING
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
WO2002096262A2 (en) 2001-05-25 2002-12-05 Northwestern University Non-alloying core shell nanoparticles
US7186814B2 (en) 2001-11-09 2007-03-06 Nanosphere, Inc. Bioconjugate-nanoparticle probes
FR2841156B1 (en) * 2002-06-19 2004-09-10 Bio Merieux NOVEL FLUORESCENT MAGNETIC PARTICLES, AS WELL AS CONJUGATES OF SUCH PARTICLES, AND DIAGNOSTIC, THERAPEUTIC OR PROPHYLACTIC COMPOSITIONS CONTAINING THEM
US7153687B2 (en) 2002-08-13 2006-12-26 Hong Kong Dna Chips Limited Apparatus and methods for detecting DNA in biological samples
JP4732683B2 (en) * 2003-12-29 2011-07-27 ユニバーサル・バイオ・リサーチ株式会社 Target substance detection method
ES2242528B1 (en) * 2004-03-25 2006-12-01 Consejo Sup. Investig. Cientificas MAGNETIC NANOPARTICLES OF NOBLE METALS.
JP5398982B2 (en) * 2004-05-24 2014-01-29 ミダテック リミテッド Nanoparticles containing RNA ligands
JP2006322709A (en) * 2005-05-17 2006-11-30 Institute Of Physical & Chemical Research Substance immobilization substrate
JP4821219B2 (en) * 2005-09-02 2011-11-24 ニプロ株式会社 Qualitative reaction specimen
KR100816419B1 (en) * 2005-10-14 2008-03-27 래플진(주) Method for Isothermal Amplification of Nucleic Acids and Method for Detecting Nucleic Acids Using Simultaneous Isothermal Amplification of Nucleic Acids and Signal Probe
WO2008018566A1 (en) * 2006-08-10 2008-02-14 The Furukawa Electric Co., Ltd. Colloidal silica particle containing light-absorbing substance, nano light-absorbing material, absorption labeling nanobead kit, and method for detection or quantification of biological molecule using the colloidal silica particle containing light-absorbing substance
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2659275B1 (en) * 2010-12-23 2017-11-22 Roche Diagnostics GmbH Detection of a polypeptide dimer by a bivalent binding agent
WO2012085064A1 (en) 2010-12-23 2012-06-28 Roche Diagnostics Gmbh Detection of a posttranslationally modified polypeptide by a bi-valent binding agent
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
KR102758333B1 (en) 2013-08-28 2025-01-23 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
JP6940484B2 (en) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド Methods and compositions for library normalization
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CN109791157B (en) 2016-09-26 2022-06-07 贝克顿迪金森公司 Measuring protein expression using reagents with barcoded oligonucleotide sequences
CN110382708A (en) 2017-02-01 2019-10-25 赛卢拉研究公司 Selective amplification using blocking oligonucleotides
WO2018165566A1 (en) 2017-03-09 2018-09-13 Auburn University Differential circuit for background correction in electrochemical measurements
CN110719959B (en) 2017-06-05 2021-08-06 贝克顿迪金森公司 Sample Indexing for Single Cells
US11505799B2 (en) 2017-07-07 2022-11-22 Innamed, Inc. Aptamers for measuring lipoprotein levels
CN112272710A (en) 2018-05-03 2021-01-26 贝克顿迪金森公司 High-throughput multi-omics sample analysis
JP7358388B2 (en) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー Molecular barcoding at opposite transcript ends
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
JP7158969B2 (en) * 2018-09-18 2022-10-24 株式会社東芝 Organic probes and molecular detectors
ES2992135T3 (en) 2018-10-01 2024-12-09 Becton Dickinson Co Determine 5’ transcription sequences
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
ES2945227T3 (en) 2019-01-23 2023-06-29 Becton Dickinson Co Antibody Associated Oligonucleotides
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
CN120099137A (en) 2019-07-22 2025-06-06 贝克顿迪金森公司 Single-cell chromatin immunoprecipitation sequencing assay
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WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (en) 2020-12-15 2023-09-01 贝克顿迪金森公司 Single-cell secretome analysis
KR102306097B1 (en) * 2021-07-13 2021-09-28 (주)바이오메트릭스 테크놀로지 Highly sensitive immunoconjugate, preparing method thereof, in vitro diagnostic reagent and in vitro diagnostic kit including the same
US12092578B2 (en) 2022-03-29 2024-09-17 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993025709A1 (en) * 1992-06-09 1993-12-23 Medical Research Council Preparation of nucleic acids
WO1998004740A1 (en) * 1996-07-29 1998-02-05 Nanosphere Llc Nanoparticles having oligonucleotides attached thereto and uses therefor
WO1998017317A2 (en) * 1996-10-23 1998-04-30 Skw Trostberg Aktiengesellschaft Process for producing biologically active polymer nanoparticle-nucleic acid conjugates
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
WO1999060169A1 (en) * 1998-05-20 1999-11-25 Molecular Machines, Inc. Multimolecular devices, drug delivery systems and single-molecule selection

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
WO2001053799A1 (en) * 2000-01-24 2001-07-26 Nanogen, Inc. Systems and devices for photoelectrophoretic transport and hybridization of oligonucleotides

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993025709A1 (en) * 1992-06-09 1993-12-23 Medical Research Council Preparation of nucleic acids
WO1998004740A1 (en) * 1996-07-29 1998-02-05 Nanosphere Llc Nanoparticles having oligonucleotides attached thereto and uses therefor
WO1998017317A2 (en) * 1996-10-23 1998-04-30 Skw Trostberg Aktiengesellschaft Process for producing biologically active polymer nanoparticle-nucleic acid conjugates
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
WO1999060169A1 (en) * 1998-05-20 1999-11-25 Molecular Machines, Inc. Multimolecular devices, drug delivery systems and single-molecule selection

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LETSINGER ET AL: "Chemistry of oligonucleotide -gold nanoparticle conjugates", PHOSPHORUS, SULFUR AND SILICON AND THE RELATED ELEMENTS (1999), 144-146, 359-362, XP008019085 *
LETSINGER R L ET AL: "Use of a steroid cyclic disulfide anchor in constructing gold nanoparticle- oligonucleotide conjugates.", BIOCONJUGATE CHEMISTRY, (2000 MAR-APR) 11 (2) 289-91., XP002205939 *
LI ZHI ET AL: "Multiple thiol-anchor capped DNA -gold nanoparticle conjugates.", NUCLEIC ACIDS RESEARCH, (2002 APR 1) 30 (7) 1558-62., XP002205940 *
See also references of EP1362121A2 *

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7259252B2 (en) 1996-07-29 2007-08-21 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US8323888B2 (en) 1996-07-29 2012-12-04 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6969761B2 (en) 1996-07-29 2005-11-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US7491496B2 (en) 2004-04-01 2009-02-17 Seiko Epson Corporation Method for immobilizing nucleic acid and method for manufacturing biosensor using the same method
US11618790B2 (en) 2010-12-23 2023-04-04 Hoffmann-La Roche Inc. Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery

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JP2004525607A (en) 2004-08-26
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