WO2002018643A3 - Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees - Google Patents

Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees Download PDF

Info

Publication number
WO2002018643A3
WO2002018643A3 PCT/US2001/025237 US0125237W WO0218643A3 WO 2002018643 A3 WO2002018643 A3 WO 2002018643A3 US 0125237 W US0125237 W US 0125237W WO 0218643 A3 WO0218643 A3 WO 0218643A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
methods
nucleic acid
provides
oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/025237
Other languages
English (en)
Other versions
WO2002018643A2 (fr
WO2002018643A9 (fr
Inventor
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Thomas Andrew Taton
Viswanadham Garimella
Zhi Li
So-Jung Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanosphere LLC
Original Assignee
Nanosphere LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/760,500 external-priority patent/US6767702B2/en
Priority claimed from US09/820,279 external-priority patent/US6750016B2/en
Priority to EP01959723A priority Critical patent/EP1362121A2/fr
Application filed by Nanosphere LLC filed Critical Nanosphere LLC
Priority to JP2002522548A priority patent/JP2004525607A/ja
Priority to CA002418502A priority patent/CA2418502A1/fr
Priority to AU2001281248A priority patent/AU2001281248A1/en
Publication of WO2002018643A2 publication Critical patent/WO2002018643A2/fr
Anticipated expiration legal-status Critical
Publication of WO2002018643A3 publication Critical patent/WO2002018643A3/fr
Publication of WO2002018643A9 publication Critical patent/WO2002018643A9/fr
Priority to AU2006200274A priority patent/AU2006200274A1/en
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Composite Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des procédés de détection d'acide nucléique. Ces procédés consistent à mettre en contact l'acide nucléique avec un ou plusieurs types de particules sur lesquels sont attachés des oligonucléotides. Dans une réalisation du procédé, les oligonucléotides sont attachés à des nanoparticules et contiennent des séquences complémentaires des portions de la séquence de l'acide nucléique. Un changement détectable (de préférence un changement de couleur) s'opère comme résultat de l'hybridation entre l'acide nucléique et les oligonucléotides portés par les nanoparticules. L'invention concerne en outre des compositions et des nécessaires contenant des nanoparticules. L'invention concerne aussi des procédés de synthèse de conjugués uniques nanoparticule-oligonucléotide, les conjugués produits à l'aide de ces procédés, et des procédés d'utilisation de ces conjugués. Elle concerne encore des nanomatériaux et des nanostructures comprenant des nanoparticules ainsi que des procédés de nanofabrication utilisant ces nanoparticules. Elle concerne enfin un procédé de séparation d'un acide nucléique sélectionné parmi d'autres acides nucléiques.
PCT/US2001/025237 2000-08-11 2001-08-10 Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees Ceased WO2002018643A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2001281248A AU2001281248A1 (en) 2000-08-11 2001-08-10 Nanoparticles having oligonucleotides attached thereto and uses therefor
CA002418502A CA2418502A1 (fr) 2000-08-11 2001-08-10 Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees
EP01959723A EP1362121A2 (fr) 2000-08-11 2001-08-10 Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees
JP2002522548A JP2004525607A (ja) 2000-08-11 2001-08-10 オリゴヌクレオチドを付着させたナノ粒子とその使用方法
AU2006200274A AU2006200274A1 (en) 2000-08-11 2006-01-23 Nanoparticles Having Oligonucleotides Attached thereto and uses therefor

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US22463100P 2000-08-11 2000-08-11
US60/224,631 2000-08-11
US25439200P 2000-12-08 2000-12-08
US60/254,392 2000-12-08
US25523500P 2000-12-11 2000-12-11
US60/255,235 2000-12-11
US09/760,500 US6767702B2 (en) 1996-07-29 2001-01-12 Nanoparticles having oligonucleotides attached thereto and uses therefor
US09/760,500 2001-01-12
US09/820,279 2001-03-28
US09/820,279 US6750016B2 (en) 1996-07-29 2001-03-28 Nanoparticles having oligonucleotides attached thereto and uses therefor

Publications (3)

Publication Number Publication Date
WO2002018643A2 WO2002018643A2 (fr) 2002-03-07
WO2002018643A3 true WO2002018643A3 (fr) 2003-09-04
WO2002018643A9 WO2002018643A9 (fr) 2003-11-06

Family

ID=27539880

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/025237 Ceased WO2002018643A2 (fr) 2000-08-11 2001-08-10 Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees

Country Status (5)

Country Link
EP (1) EP1362121A2 (fr)
JP (1) JP2004525607A (fr)
AU (1) AU2001281248A1 (fr)
CA (1) CA2418502A1 (fr)
WO (1) WO2002018643A2 (fr)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US7491496B2 (en) 2004-04-01 2009-02-17 Seiko Epson Corporation Method for immobilizing nucleic acid and method for manufacturing biosensor using the same method
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US11618790B2 (en) 2010-12-23 2023-04-04 Hoffmann-La Roche Inc. Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
US12630874B1 (en) 2023-03-20 2026-05-19 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
WO1998004740A1 (fr) 1996-07-29 1998-02-05 Nanosphere Llc Nanoparticules sur lesquelles sont attaches des oligonucleotides et utilisations desdites nanoparticules
WO2002004681A2 (fr) 2000-07-11 2002-01-17 Northwestern University Procede de detection par accroissement du marquage a l'argent
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7238472B2 (en) 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles
ATE403751T1 (de) 2001-11-09 2008-08-15 Nanosphere Inc Biokonjugat-nanopartikelsonden
FR2841156B1 (fr) * 2002-06-19 2004-09-10 Bio Merieux Nouvelles particules magnetiques fluorescentes,ainsi que les conjugues de telles particules et les compositions diagnostiques , therapeutiques ou prophylactiques les contenant
US7153687B2 (en) 2002-08-13 2006-12-26 Hong Kong Dna Chips Limited Apparatus and methods for detecting DNA in biological samples
JP4732683B2 (ja) * 2003-12-29 2011-07-27 ユニバーサル・バイオ・リサーチ株式会社 標的物質の検出方法
ES2242528B1 (es) * 2004-03-25 2006-12-01 Consejo Sup. Investig. Cientificas Nanoparticulas magneticas de metales nobles.
EP1756289B1 (fr) * 2004-05-24 2015-01-14 Midatech Ltd. Nanoparticules comprenant des ligands d'arn
JP2006322709A (ja) * 2005-05-17 2006-11-30 Institute Of Physical & Chemical Research 物質固定化基板
JP4821219B2 (ja) * 2005-09-02 2011-11-24 ニプロ株式会社 定性反応用試験片
KR100816419B1 (ko) * 2005-10-14 2008-03-27 래플진(주) 핵산의 등온증폭 방법 및 핵산과 신호 프로브의 동시등온증폭을 이용한 핵산의 검출방법
WO2008018566A1 (fr) * 2006-08-10 2008-02-14 The Furukawa Electric Co., Ltd. Particule de silice colloïdale contenant une substance absorbant la lumière, un nanomatériau absorbant la lumière, un kit de nanoperle de marquage d'absorption, et un procédé de détection ou de quantification de molécules biologiques utilisant la particule de silice colloïdale contenant un
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2659275B1 (fr) * 2010-12-23 2017-11-22 Roche Diagnostics GmbH Détection d'un dimère polypeptidique par un agent de liaison bivalent
CN103384825B (zh) 2010-12-23 2017-05-24 霍夫曼-拉罗奇有限公司 通过二价结合剂检测经翻译后修饰的多肽
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
CA2865575C (fr) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions et trousses pour le comptage moleculaire
US9567645B2 (en) 2013-08-28 2017-02-14 Cellular Research, Inc. Massively parallel single cell analysis
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
EP3835431B1 (fr) 2015-03-30 2022-11-02 Becton, Dickinson and Company Procédés pour codage à barres combinatoire
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
AU2017331459B2 (en) 2016-09-26 2023-04-13 Becton, Dickinson And Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
WO2018165566A1 (fr) 2017-03-09 2018-09-13 Auburn University Circuit différentiel pour correction du fond dans des mesures électrochimiques
WO2018226293A1 (fr) 2017-06-05 2018-12-13 Becton, Dickinson And Company Indexation d'échantillon pour des cellules uniques
WO2019010341A1 (fr) 2017-07-07 2019-01-10 Innamed, Inc. Aptamères pour mesurer les taux de lipoprotéines
EP3788171B1 (fr) 2018-05-03 2023-04-05 Becton, Dickinson and Company Analyse multi-omique d'échantillons à haut débit
EP3788170B1 (fr) 2018-05-03 2025-01-01 Becton, Dickinson and Company Codage à barres moléculaire sur des extrémités de transcrit en regard
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
JP7158969B2 (ja) * 2018-09-18 2022-10-24 株式会社東芝 有機物プローブおよび分子検出装置
WO2020072380A1 (fr) 2018-10-01 2020-04-09 Cellular Research, Inc. Détermination de séquences de transcripts 5'
EP3877520B1 (fr) 2018-11-08 2025-03-19 Becton, Dickson And Company Analyse transcriptomique complète de cellules uniques à l'aide d'un amorçage aléatoire
EP4745247A2 (fr) 2018-12-13 2026-05-20 Becton Dickinson Co Extension sélective dans une analyse de transcriptome entier de cellule unique
CN113574178B (zh) 2019-01-23 2024-10-29 贝克顿迪金森公司 与抗体关联的寡核苷酸
CN120099139A (zh) 2019-02-14 2025-06-06 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
EP4055160B1 (fr) 2019-11-08 2024-04-10 Becton Dickinson and Company Utilisation d'un amorçage aléatoire pour obtenir des informations v(d)j de pleine longueur pour le séquençage du répertoire immunitaire
EP4090763B1 (fr) 2020-01-13 2024-12-04 Becton Dickinson and Company Procédés et compositions pour la quantification de protéines et d'arn
WO2021146219A1 (fr) 2020-01-13 2021-07-22 Becton, Dickinson And Company Capture de cellules à l'aide d'oligonucléotides contenant de la du
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (fr) 2020-07-31 2022-02-03 Becton, Dickinson And Company Dosage à cellule unique pour chromatine accessible par transposase
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
KR102306097B1 (ko) * 2021-07-13 2021-09-28 (주)바이오메트릭스 테크놀로지 고감도 면역접합체, 이의 제조방법, 이를 포함한 체외 진단시약 및 체외 진단키트
US12092578B2 (en) 2022-03-29 2024-09-17 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993025709A1 (fr) * 1992-06-09 1993-12-23 Medical Research Council Preparation d'acides nucleiques
WO1998004740A1 (fr) * 1996-07-29 1998-02-05 Nanosphere Llc Nanoparticules sur lesquelles sont attaches des oligonucleotides et utilisations desdites nanoparticules
WO1998017317A2 (fr) * 1996-10-23 1998-04-30 Skw Trostberg Aktiengesellschaft Procede de production de conjugues biologiquement actifs d'acides nucleiques et de nanoparticules polymeres
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
WO1999060169A1 (fr) * 1998-05-20 1999-11-25 Molecular Machines, Inc. Dispositifs multimoleculaires, systemes d'administration de medicaments et selection de molecule unique

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
WO2001053799A1 (fr) * 2000-01-24 2001-07-26 Nanogen, Inc. Systemes et dispositifs de transport par electrophorese et d'hybridation d'oligonucleotides

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993025709A1 (fr) * 1992-06-09 1993-12-23 Medical Research Council Preparation d'acides nucleiques
WO1998004740A1 (fr) * 1996-07-29 1998-02-05 Nanosphere Llc Nanoparticules sur lesquelles sont attaches des oligonucleotides et utilisations desdites nanoparticules
WO1998017317A2 (fr) * 1996-10-23 1998-04-30 Skw Trostberg Aktiengesellschaft Procede de production de conjugues biologiquement actifs d'acides nucleiques et de nanoparticules polymeres
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
WO1999060169A1 (fr) * 1998-05-20 1999-11-25 Molecular Machines, Inc. Dispositifs multimoleculaires, systemes d'administration de medicaments et selection de molecule unique

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LETSINGER ET AL: "Chemistry of oligonucleotide -gold nanoparticle conjugates", PHOSPHORUS, SULFUR AND SILICON AND THE RELATED ELEMENTS (1999), 144-146, 359-362, XP008019085 *
LETSINGER R L ET AL: "Use of a steroid cyclic disulfide anchor in constructing gold nanoparticle- oligonucleotide conjugates.", BIOCONJUGATE CHEMISTRY, (2000 MAR-APR) 11 (2) 289-91., XP002205939 *
LI ZHI ET AL: "Multiple thiol-anchor capped DNA -gold nanoparticle conjugates.", NUCLEIC ACIDS RESEARCH, (2002 APR 1) 30 (7) 1558-62., XP002205940 *
See also references of EP1362121A2 *

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7259252B2 (en) 1996-07-29 2007-08-21 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US8323888B2 (en) 1996-07-29 2012-12-04 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6969761B2 (en) 1996-07-29 2005-11-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US7491496B2 (en) 2004-04-01 2009-02-17 Seiko Epson Corporation Method for immobilizing nucleic acid and method for manufacturing biosensor using the same method
US11618790B2 (en) 2010-12-23 2023-04-04 Hoffmann-La Roche Inc. Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
US12630874B1 (en) 2023-03-20 2026-05-19 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing

Also Published As

Publication number Publication date
AU2001281248A1 (en) 2002-03-13
EP1362121A2 (fr) 2003-11-19
WO2002018643A2 (fr) 2002-03-07
JP2004525607A (ja) 2004-08-26
WO2002018643A9 (fr) 2003-11-06
CA2418502A1 (fr) 2002-03-07

Similar Documents

Publication Publication Date Title
WO2001051665A3 (fr) Nanoparticules auxquelles sont attaches des oligonucleotides et utilisations de ces dernieres
WO2001073123A3 (fr) Nanoparticules auxquelles sont attaches des oligonucleotides et leur utilisation
WO2002046472A3 (fr) Nanoparticules auxquelles sont attaches des oligonucleotides et utilisations de ces dernieres
WO2003035829A3 (fr) Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules
CA2262018A1 (fr) Nanoparticules sur lesquelles sont attaches des oligonucleotides et utilisations desdites nanoparticules
WO1997018334A3 (fr) Sondes d'acides nucleiques complementaires des acides nucleiques du virus du papillome humain, methodes et preparations associees
WO2004083386A3 (fr) Procedes d'elaboration de sondes privees de sequences repetitives, et utilisations
ATE502117T1 (de) Nanopartikel mit gebundenen oligonukleotiden und deren verwendungen
CA2172722A1 (fr) Procedes et compositions pour le sequencage efficace d'acide nucleique
DE602005020943D1 (de) Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe
WO1999049082A3 (fr) Nucleotides modifies et methodes utiles pour le sequençage d'acides nucleiques
WO2004072238A3 (fr) Nucleotides marques par des phosphates terminaux, presentant de nouveaux lieurs
WO2002016594A3 (fr) Procedes et reactifs de clonage moleculaire
WO2003089906A3 (fr) Nanoparticules fonctionnalisees et procede d'utilisation
WO2001098537A3 (fr) Sites d'hybridation accessibles a l'acide nucleique
WO2001079563A3 (fr) Sondes d'acides nucleiques degradables et methodes de detection d'acides nucleiques
WO2004072235A3 (fr) Evenement mon 88913 de plant de coton et procedes de detection correspondants
AU3728897A (en) Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific Tm
WO2002061133A3 (fr) Detection d'acide nucleique avec des sondes structurees
WO2002022890A3 (fr) Compositions, procedes et trousses pour determiner la presence d'organismes du genre cryptosporidium dans un echantillon a analyser
WO2004020654A3 (fr) Dosages d'acide nucleique en rapport avec une phase solide, combinant une haute affinite avec une haute specificite
CA2319757A1 (fr) Procede de detection de sequences cibles d'acide nucleique impliquant la transcription in vitro a partir d'un promoteur d'arn
WO2002034951A3 (fr) Compositions et procedes de detection du virus de l'immunodeficience humaine 2 (vih-2)
WO2005047468A3 (fr) Procedes ameliores pour la detection et la mesure de sequences d'acides nucleiques specifiques
EP2090652A4 (fr) Procede de detection du virus latent du houblon, ensemble d'amorces et necessaire destines a cette detection

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2418502

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2001281248

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2002522548

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2001959723

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

COP Corrected version of pamphlet

Free format text: PAGES 1/64-64/64, DRAWINGS, REPLACED BY NEW PAGES 1/64-64/64 ; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

WWP Wipo information: published in national office

Ref document number: 2001959723

Country of ref document: EP