WO2002024917A3 - Method for modifying the genome of corynebacteria - Google Patents

Method for modifying the genome of corynebacteria Download PDF

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Publication number
WO2002024917A3
WO2002024917A3 PCT/EP2001/010805 EP0110805W WO0224917A3 WO 2002024917 A3 WO2002024917 A3 WO 2002024917A3 EP 0110805 W EP0110805 W EP 0110805W WO 0224917 A3 WO0224917 A3 WO 0224917A3
Authority
WO
WIPO (PCT)
Prior art keywords
corynebacteria
genome
modifying
replicate
foreign
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/010805
Other languages
German (de)
French (fr)
Other versions
WO2002024917A2 (en
Inventor
Markus Pompejus
Hartwig Schroeder
Burkhard Kroeger
Oskar Zelder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
BASF SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE filed Critical BASF SE
Priority to EP01982319A priority Critical patent/EP1322766A2/en
Priority to AU2002213937A priority patent/AU2002213937A1/en
Priority to CA002422743A priority patent/CA2422743A1/en
Priority to JP2002529509A priority patent/JP2004509625A/en
Priority to KR10-2003-7003966A priority patent/KR20030074597A/en
Publication of WO2002024917A2 publication Critical patent/WO2002024917A2/en
Publication of WO2002024917A3 publication Critical patent/WO2002024917A3/en
Anticipated expiration legal-status Critical
Priority to US11/055,717 priority patent/US20080131942A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to a method for producing corynebacteria that contain one or more modified genomic sequences, characterized by using a vector that does not replicate in corynebacteria and whose nucleic acid is not recognized as foreign by the corynebacteria.
PCT/EP2001/010805 2000-09-20 2001-09-19 Method for modifying the genome of corynebacteria Ceased WO2002024917A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP01982319A EP1322766A2 (en) 2000-09-20 2001-09-19 Method for modifying the genome of corynebacteria
AU2002213937A AU2002213937A1 (en) 2000-09-20 2001-09-19 Method for modifying the genome of corynebacteria
CA002422743A CA2422743A1 (en) 2000-09-20 2001-09-19 Method for modifying the genome of corynebacteria
JP2002529509A JP2004509625A (en) 2000-09-20 2001-09-19 Method for modifying the genome of Corynebacterium
KR10-2003-7003966A KR20030074597A (en) 2000-09-20 2001-09-19 Method for Modifying the Genome of Corynebacteria
US11/055,717 US20080131942A1 (en) 2000-09-20 2005-02-11 Process for modifying the genome of cornynebacteria

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10046870.5 2000-09-20
DE10046870A DE10046870A1 (en) 2000-09-20 2000-09-20 Genetic manipulation of corynebacteria, useful for preparing fine chemicals, using a non-replicable vector that is not recognized as foreign

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/055,717 Continuation US20080131942A1 (en) 2000-09-20 2005-02-11 Process for modifying the genome of cornynebacteria

Publications (2)

Publication Number Publication Date
WO2002024917A2 WO2002024917A2 (en) 2002-03-28
WO2002024917A3 true WO2002024917A3 (en) 2002-06-27

Family

ID=7657155

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/010805 Ceased WO2002024917A2 (en) 2000-09-20 2001-09-19 Method for modifying the genome of corynebacteria

Country Status (8)

Country Link
US (2) US20030170775A1 (en)
EP (2) EP1322766A2 (en)
JP (1) JP2004509625A (en)
KR (1) KR20030074597A (en)
AU (1) AU2002213937A1 (en)
CA (1) CA2422743A1 (en)
DE (1) DE10046870A1 (en)
WO (1) WO2002024917A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10217058A1 (en) 2002-04-17 2003-11-27 Basf Ag Process for the production of fine chemicals containing sulfur
DE10222858A1 (en) 2002-05-23 2003-12-04 Basf Ag Process for the fermentative production of sulfur-containing fine chemicals
DE10239073A1 (en) 2002-08-26 2004-03-11 Basf Ag Fermentative production of sulfur-containing fine chemicals, useful e.g. as feed additive, by culturing bacteria containing heterologous sequence for homoserine O-acetyltransferase
DE10239082A1 (en) 2002-08-26 2004-03-04 Basf Ag Fermentative production of sulfur-containing fine chemicals, useful e.g. as feed additive, by culturing bacteria containing heterologous sequence for O-acetylhomoserine sulfhydrolase
DE10239308A1 (en) 2002-08-27 2004-03-11 Basf Ag Fermentative production of sulfur-containing fine chemicals, useful e.g. as feed additive, by culturing bacteria containing heterologous sequence for methionine synthase
KR20060125804A (en) 2003-12-18 2006-12-06 바스프 악티엔게젤샤프트 Methods for the preparation of lysine by fermentation of corynebacterium glutamicum
DE10359660A1 (en) 2003-12-18 2005-07-28 Basf Ag Psod expression units
DE10359594A1 (en) 2003-12-18 2005-07-28 Basf Ag PEF TU-expression units
DE10359595A1 (en) 2003-12-18 2005-07-28 Basf Ag Pgro expression units
DE102004061846A1 (en) 2004-12-22 2006-07-13 Basf Ag Multiple promoters
KR100694427B1 (en) * 2005-12-02 2007-03-12 씨제이 주식회사 Microorganisms of Corynebacterium and Preparation Method of 5'-inosinic Acid Using the Same
PL2082045T3 (en) 2006-10-24 2015-07-31 Basf Se Method of reducing gene expression using modified codon usage
PT2164975E (en) 2007-07-06 2012-03-08 Basf Se Process for preparing a concentrated aqueous glucose solution from corn
KR100957689B1 (en) * 2008-01-18 2010-05-12 씨제이제일제당 (주) Microorganisms of the genus Corynebacterium with improved production capacity of 5'-guanosine monophosphate and method for producing 5'-guanosine monophosphate
US9963709B2 (en) * 2012-09-14 2018-05-08 Uchicago Argonne, Llc Transformable Rhodobacter strains, method for producing transformable Rhodobacter strains
JP2019050731A (en) * 2016-01-15 2019-04-04 三島光産株式会社 Method for promoting growth of organisms performing oxygen-generating photosynthesis
CN111073841A (en) * 2019-11-25 2020-04-28 华农（肇庆）生物产业技术研究院有限公司 Corynebacterium ATCC13032 improved strain capable of effectively expressing foreign protein and construction method thereof
KR102706898B1 (en) * 2022-06-07 2024-09-19 씨제이제일제당 주식회사 Microorganism of the genus Corynebacterium with enhanced riboflavin productivity and a method of producing riboflavin using the same
KR102879056B1 (en) * 2023-01-27 2025-10-30 씨제이제일제당 (주) Microorganism having enhanced activity of pantoate-beta-alanine ligase from glutamicibacter halophytocola and uses thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002627A1 (en) * 1990-08-08 1992-02-20 Centre National De La Recherche Scientifique (Cnrs) Corynebacteria integron, method of transformation of corynebacteria by said integron, and corynebacteria obtained
US5683900A (en) * 1993-12-06 1997-11-04 The United States Of America As Represented By The Department Of Agriculture Pasteurella haemolytica PhaI restriction endonuclcape and methyltranstesase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0693558B1 (en) 1994-07-19 2002-12-04 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Trehalose and its production and use
DE19823834A1 (en) 1998-05-28 1999-12-02 Basf Ag Genetic process for the production of riboflavin

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002627A1 (en) * 1990-08-08 1992-02-20 Centre National De La Recherche Scientifique (Cnrs) Corynebacteria integron, method of transformation of corynebacteria by said integron, and corynebacteria obtained
US5683900A (en) * 1993-12-06 1997-11-04 The United States Of America As Represented By The Department Of Agriculture Pasteurella haemolytica PhaI restriction endonuclcape and methyltranstesase

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DESOMER J ET AL: "ILLEGITIMATE INTEGRATION OF NON-REPLICATIVE VECTORS IN THE GENOME OF RHODOCOCCUS-FASCIANS UPON ELECTROTRANSFORMATION AS AN INSERTIONAL MUTAGENESIS SYSTEM", MOLECULAR MICROBIOLOGY, vol. 5, no. 9, 1991, pages 2115 - 2124, XP002194561, ISSN: 0950-382X *
DONAHUE, J. P. ET AL.: "Overcoming the restriction barrier to plasmid transformation of Helicobacter pylori", MOL. MICROBIOLOGY, vol. 37, no. 5, 30 August 2000 (2000-08-30), pages 1066 - 1074, XP002194559 *
DUNICAN L K ET AL: "HIGH FREQUENCY TRANSFORMATION OF WHOLE CELLS OF AMINO ACID PRODUCING CORYNEFORM BACTERIA USING HIGH VOLTAGE ELECTROPORATION", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 7, no. 10, 1 October 1989 (1989-10-01), pages 1067 - 1070, XP000054648, ISSN: 0733-222X *
KIM, Y. H., LEE, H-S.: "Site-specific disruption of glyoxylate bypass and its effect in lysine-producing Corynebacterium lactofermentum strain", J. MICROBIOL. BIOTECHNOL., vol. 6, no. 5, 1996, pages 315 - 320, XP002194560 *
SCHAEFER A ET AL: "The Corynebacterium glutamicum cglIM gene encoding a 5-cytosine methyltransferase enzyme confers a specific DNA methylation pattern in an McrBC-deficient Escherichia coli strain.", GENE, (1997 DEC 12) 203 (2) 95-101, XP001056557 *

Also Published As

Publication number Publication date
CA2422743A1 (en) 2003-03-18
KR20030074597A (en) 2003-09-19
JP2004509625A (en) 2004-04-02
EP1921151A1 (en) 2008-05-14
DE10046870A1 (en) 2002-03-28
AU2002213937A1 (en) 2002-04-02
EP1322766A2 (en) 2003-07-02
WO2002024917A2 (en) 2002-03-28
US20030170775A1 (en) 2003-09-11
US20080131942A1 (en) 2008-06-05

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