WO2002024959A3 - Multiple reporter read-out for bioassays - Google Patents

Multiple reporter read-out for bioassays Download PDF

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Publication number
WO2002024959A3
WO2002024959A3 PCT/US2001/029743 US0129743W WO0224959A3 WO 2002024959 A3 WO2002024959 A3 WO 2002024959A3 US 0129743 W US0129743 W US 0129743W WO 0224959 A3 WO0224959 A3 WO 0224959A3
Authority
WO
WIPO (PCT)
Prior art keywords
addressable
alleles
detecting
analyte
fluorescence intensity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/029743
Other languages
French (fr)
Other versions
WO2002024959A2 (en
Inventor
James W Jacobson
Jennifer L Burroughs
Kerry G Oliver
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Luminex Corp
Original Assignee
Luminex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Luminex Corp filed Critical Luminex Corp
Priority to AU2001294648A priority Critical patent/AU2001294648A1/en
Publication of WO2002024959A2 publication Critical patent/WO2002024959A2/en
Anticipated expiration legal-status Critical
Publication of WO2002024959A3 publication Critical patent/WO2002024959A3/en
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Reproductive Health (AREA)
  • Nanotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A method for detecting a plurality of reactive sites on an analyte, comprising allowing reactants on an addressable microsphere and the reactive sites to react, forming reactant-reactive site pairs distinguishable by fluorescence intensity. The invention also provides a method for detecitng a plurality of analytes in a sample using addressable microspheres in combination with one or more reporter reagents. Also provided are a method for determining allele zygosity of a genetic locus having two alleles or more alleles using microparticles, and a method for detecting a plurality of SNPs in nucleic acid molecules. The instant invention also provides a composition comprising an addressable microsphere carrying at least two fluorescent reactants capable of forming reactant-analyte pairs distinguishable by their fluorescence intensity, and kits comprising the inventive composition and a pluraltiy of reporter reagents.
PCT/US2001/029743 2000-09-22 2001-09-24 Multiple reporter read-out for bioassays Ceased WO2002024959A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001294648A AU2001294648A1 (en) 2000-09-22 2001-09-24 Multiple reporter read-out for bioassays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23434000P 2000-09-22 2000-09-22
US60/234,340 2000-09-22

Publications (2)

Publication Number Publication Date
WO2002024959A2 WO2002024959A2 (en) 2002-03-28
WO2002024959A3 true WO2002024959A3 (en) 2003-08-21

Family

ID=22880953

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/029743 Ceased WO2002024959A2 (en) 2000-09-22 2001-09-24 Multiple reporter read-out for bioassays

Country Status (2)

Country Link
AU (1) AU2001294648A1 (en)
WO (1) WO2002024959A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7217518B2 (en) 2002-08-02 2007-05-15 Applera Corporation Fluorescence polarization assay
US7260485B2 (en) * 2003-07-18 2007-08-21 Luminex Corporation Method and systems for distinguishing between materials having similar spectra
US20080318340A1 (en) * 2004-11-01 2008-12-25 Tokyo Metropolitan Organization For Medical Resear Method of Detecting Target Substances
JP4974899B2 (en) * 2004-12-10 2012-07-11 ジェネラ バイオシステムズ リミテッド Composition and detection method
CN101341257B (en) * 2004-12-10 2012-10-10 杰纳罗生物系统有限责任公司 Human papilloma virus (HPV) detection using nucleic acid probes, microbeads and fluorescent-activated cell sorter (FACS)
WO2008074182A1 (en) * 2006-12-18 2008-06-26 Shanghai Tellgen Life Science Co., Ltd. Method for the detection and typing of human papillomavirus, and the reagent kit thereof
EP2443256A1 (en) 2009-06-15 2012-04-25 BG Research Ltd Nucleic acid detection
CN102507948B (en) * 2011-11-15 2013-12-04 吉林出入境检验检疫局检验检疫技术中心 Method using liquid phase chip to detect mononuclear cell proliferation listeria
CN113514437A (en) * 2021-06-24 2021-10-19 重庆大学 High-sensitivity fluorescence detection method and kit for epinephrine
CN114957293B (en) * 2022-05-30 2023-07-25 常州大学 Guanine-based cyanine probe for specific recognition of insulin and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5736330A (en) * 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5736330A (en) * 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FULTON R J ET AL: "Advanced multiplexd analysis with the FlowMetrix(TM) system", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY. WINSTON, US, vol. 43, no. 9, September 1997 (1997-09-01), pages 1749 - 1756, XP002142645, ISSN: 0009-9147 *
KETTMAN J R ET AL: "CLASSIFICATION AND PROPERTIES OF 64 MULTIPLEXED MICROSPHERE SETS", CYTOMETRY, ALAN R. LISS, INC, XX, vol. 33, no. 2, 1 October 1998 (1998-10-01), pages 234 - 243, XP001041450, ISSN: 0196-4763 *
SMITH P L ET AL: "A RAPID, SENSITIVE, MULTIPLEXED ASSAY FOR DETECTION OF VIRAL NUCLEIC ACIDS USING THE FLOW METRIX SYSTEM", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY. WINSTON, US, vol. 44, no. 9, 1998, pages 2054 - 2056, XP002170109, ISSN: 0009-9147 *
TYAGI S ET AL: "Multicolor molecular beacons for allele discrimination", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 16, 1998, pages 49 - 53, XP002143901, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2002024959A2 (en) 2002-03-28
AU2001294648A1 (en) 2002-04-02

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