WO2002052272A3 - Screening-verfahren zum nachweis von reversiblen protein-protein wechselwirkungen - Google Patents

Screening-verfahren zum nachweis von reversiblen protein-protein wechselwirkungen Download PDF

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Publication number
WO2002052272A3
WO2002052272A3 PCT/EP2001/015265 EP0115265W WO02052272A3 WO 2002052272 A3 WO2002052272 A3 WO 2002052272A3 EP 0115265 W EP0115265 W EP 0115265W WO 02052272 A3 WO02052272 A3 WO 02052272A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
technique
screening method
detecting
interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/015265
Other languages
English (en)
French (fr)
Other versions
WO2002052272A2 (de
Inventor
Oliver Rocks
Alfred Wittinghofer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evotec OAI AG
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Evotec OAI AG
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec OAI AG, Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Evotec OAI AG
Priority to AU2002238479A priority Critical patent/AU2002238479A1/en
Priority to EP01986933A priority patent/EP1370868A2/de
Publication of WO2002052272A2 publication Critical patent/WO2002052272A2/de
Anticipated expiration legal-status Critical
Publication of WO2002052272A3 publication Critical patent/WO2002052272A3/de
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Gegenstand der Erfindung sind Verfahren und Assays zum Nachweis von Protein-Protein Wechselwirkungen und zum testen von Wirkstoffen in Expressionssystemen wie Zellen, wobei ein Protein I mit einem Protein II interagiert, wobei ein Transfer des Protein I in ein Zellkompartiment erfolgt, in dem sowohl Protein I als auch Protein II natürlicherweise nicht vorkommen, und bei Nachweis von Protein II in dem Kompartiment, in das Protein I transferiert wurde, eine Wechselwirkung zwischen Protein I und Protein II festgestellt wird.
PCT/EP2001/015265 2000-12-23 2001-12-21 Screening-verfahren zum nachweis von reversiblen protein-protein wechselwirkungen Ceased WO2002052272A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002238479A AU2002238479A1 (en) 2000-12-23 2001-12-21 Technique and screening method for detecting reversible protein-protein interactions
EP01986933A EP1370868A2 (de) 2000-12-23 2001-12-21 Methode und screening-verfahren zum nachweis von reversiblen protein-protein wechselwirkungen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10064972 2000-12-23
DE10064972.6 2000-12-23

Publications (2)

Publication Number Publication Date
WO2002052272A2 WO2002052272A2 (de) 2002-07-04
WO2002052272A3 true WO2002052272A3 (de) 2003-07-10

Family

ID=7668947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/015265 Ceased WO2002052272A2 (de) 2000-12-23 2001-12-21 Screening-verfahren zum nachweis von reversiblen protein-protein wechselwirkungen

Country Status (3)

Country Link
EP (1) EP1370868A2 (de)
AU (1) AU2002238479A1 (de)
WO (1) WO2002052272A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8114615B2 (en) 2006-05-17 2012-02-14 Cernostics, Inc. Method for automated tissue analysis

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006017751A2 (en) * 2004-08-02 2006-02-16 Cellumen, Inc. Methods for the detection of molecular interactions within cells
US10018631B2 (en) 2011-03-17 2018-07-10 Cernostics, Inc. Systems and compositions for diagnosing Barrett's esophagus and methods of using the same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000005410A2 (en) * 1998-07-22 2000-02-03 Technion Research And Development Foundation Ltd. Method for detecting protein-protein interactions and a kit therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000005410A2 (en) * 1998-07-22 2000-02-03 Technion Research And Development Foundation Ltd. Method for detecting protein-protein interactions and a kit therefor

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
PERANDER MARIA ET AL: "Nuclear import and export signals enable rapid nucleocytoplasmic shuttling of the atypical protein kinase C lambda.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 16, 13 December 2000 (2000-12-13), pages 13015 - 13024, XP002175456, ISSN: 0021-9258 *
RHEE YOON ET AL: "A genetic system for detection of protein nuclear import and export", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 18, no. 4, April 2000 (2000-04-01), pages 433 - 437, XP002168963, ISSN: 1087-0156 *
SCHNEIDER STEFAN ET AL: "Mutagenesis and selection of PDZ domains that bind new protein targets.", NATURE BIOTECHNOLOGY, vol. 17, no. 2, February 1999 (1999-02-01), pages 170 - 175, XP002175455, ISSN: 1087-0156 *
ZHANG MING JIE ET AL: "Tolerance of diverse amino acid substitutions at conserved positions in the nuclear export signal (NES) of HIV-1 Rev.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 243, no. 1, 4 February 1998 (1998-02-04), pages 113 - 116, XP002175457, ISSN: 0006-291X *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8114615B2 (en) 2006-05-17 2012-02-14 Cernostics, Inc. Method for automated tissue analysis
US8597899B2 (en) 2006-05-17 2013-12-03 Cernostics, Inc. Method for automated tissue analysis

Also Published As

Publication number Publication date
AU2002238479A1 (en) 2002-07-08
WO2002052272A2 (de) 2002-07-04
EP1370868A2 (de) 2003-12-17

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