WO2002072846A3 - Genes synthetiques et plasmides bacteriens depourvus de cpg - Google Patents

Genes synthetiques et plasmides bacteriens depourvus de cpg Download PDF

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Publication number
WO2002072846A3
WO2002072846A3 PCT/FR2002/000862 FR0200862W WO02072846A3 WO 2002072846 A3 WO2002072846 A3 WO 2002072846A3 FR 0200862 W FR0200862 W FR 0200862W WO 02072846 A3 WO02072846 A3 WO 02072846A3
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WO
WIPO (PCT)
Prior art keywords
cpg
synthetic genes
bacterial plasmids
devoid
plasmids devoid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2002/000862
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English (en)
Other versions
WO2002072846A2 (fr
WO2002072846A9 (fr
Inventor
Daniel Drocourt
Jean-Paul Reynes
Gerard Tiraby
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cayla
Original Assignee
Cayla
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8860962&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2002072846(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to US10/469,851 priority Critical patent/US7244609B2/en
Priority to JP2002571897A priority patent/JP4264703B2/ja
Priority to DE60221801T priority patent/DE60221801T3/de
Priority to AU2002253226A priority patent/AU2002253226B2/en
Priority to CA2440195A priority patent/CA2440195C/fr
Application filed by Cayla filed Critical Cayla
Priority to EP02722336A priority patent/EP1366176B2/fr
Publication of WO2002072846A2 publication Critical patent/WO2002072846A2/fr
Publication of WO2002072846A3 publication Critical patent/WO2002072846A3/fr
Publication of WO2002072846A9 publication Critical patent/WO2002072846A9/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente demande est relative à une série de vecteurs plasmidiques bactériens entiérement dépourvus de CpG qui peuvent exprimer chez la bactérie Escherichia coli des gènes synthétiques ne contenant pas de CpG.
PCT/FR2002/000862 2001-03-09 2002-03-11 Genes synthetiques et plasmides bacteriens depourvus de cpg Ceased WO2002072846A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP02722336A EP1366176B2 (fr) 2001-03-09 2002-03-11 Genes synthetiques et plasmides bacteriens depourvus de cpg
JP2002571897A JP4264703B2 (ja) 2001-03-09 2002-03-11 合成遺伝子、およびＣｐＧを欠く細菌プラスミド
DE60221801T DE60221801T3 (de) 2001-03-09 2002-03-11 Cpg-freie synthetische gene und bakterielle plasmide
AU2002253226A AU2002253226B2 (en) 2001-03-09 2002-03-11 Synthetic genes and bacterial plasmids devoid of CpG
CA2440195A CA2440195C (fr) 2001-03-09 2002-03-11 Genes synthetiques et plasmides bacteriens depourvus de cpg
US10/469,851 US7244609B2 (en) 2001-03-09 2002-03-11 Synthetic genes and bacterial plasmids devoid of CpG

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR01/03274 2001-03-09
FR0103274A FR2821855B1 (fr) 2001-03-09 2001-03-09 Genes synthetiques et plasmides bacteriens depourvus de cpg

Publications (3)

Publication Number Publication Date
WO2002072846A2 WO2002072846A2 (fr) 2002-09-19
WO2002072846A3 true WO2002072846A3 (fr) 2003-02-27
WO2002072846A9 WO2002072846A9 (fr) 2003-03-20

Family

ID=8860962

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/000862 Ceased WO2002072846A2 (fr) 2001-03-09 2002-03-11 Genes synthetiques et plasmides bacteriens depourvus de cpg

Country Status (9)

Country Link
US (1) US7244609B2 (fr)
EP (1) EP1366176B2 (fr)
JP (1) JP4264703B2 (fr)
AT (1) ATE370238T1 (fr)
AU (1) AU2002253226B2 (fr)
CA (1) CA2440195C (fr)
DE (1) DE60221801T3 (fr)
FR (1) FR2821855B1 (fr)
WO (1) WO2002072846A2 (fr)

Families Citing this family (37)

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Publication number Priority date Publication date Assignee Title
FR2821855B1 (fr) † 2001-03-09 2004-04-02 Cayla Genes synthetiques et plasmides bacteriens depourvus de cpg
AU2002317771A1 (en) * 2001-06-05 2002-12-16 Cellectis Methods for modifying the cpg content of polynucleotides
DK1572994T3 (da) 2002-12-20 2007-05-29 Chromagenics Bv Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer
NZ553700A (en) 2004-11-08 2010-09-30 Chromagenics Bv Selection of host cells expressing protein at high levels using a selectable marker with a non-optimal start codon
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US8039230B2 (en) * 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
BRPI0517380A (pt) * 2004-11-08 2008-10-07 Chromagenics Bv molécula de dna, cassete de expressão, célula hospedeira, e, métodos para gerar uma célula hospedeira que expressa um polipeptìdeo de interesse e para produzir um polipeptìdeo de interesse
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
GB0606190D0 (en) 2006-03-28 2006-05-10 Isis Innovation Construct
AU2009255947B2 (en) 2008-06-06 2014-12-18 Aurora Algae, Inc. Vcp-based vectors for algal cell transformation
US20100022393A1 (en) * 2008-07-24 2010-01-28 Bertrand Vick Glyphosate applications in aquaculture
US8314228B2 (en) 2009-02-13 2012-11-20 Aurora Algae, Inc. Bidirectional promoters in Nannochloropsis
US8865468B2 (en) * 2009-10-19 2014-10-21 Aurora Algae, Inc. Homologous recombination in an algal nuclear genome
US8809046B2 (en) 2011-04-28 2014-08-19 Aurora Algae, Inc. Algal elongases
US9029137B2 (en) 2009-06-08 2015-05-12 Aurora Algae, Inc. ACP promoter
WO2011011463A2 (fr) * 2009-07-20 2011-01-27 Aurora Biofuels, Inc. Manipulation d'une voie respiratoire alternative chez les photo-autotrophes
US20120213848A1 (en) * 2010-11-16 2012-08-23 Hanley Brian P Treatment of Infection Using Single Chain Antibody Gene Therapy
US8722359B2 (en) 2011-01-21 2014-05-13 Aurora Algae, Inc. Genes for enhanced lipid metabolism for accumulation of lipids
AU2012249377A1 (en) 2011-04-28 2013-11-14 Aurora Algae, Inc. Algal desaturases
US8435777B1 (en) 2012-01-20 2013-05-07 Cayla CPG-free gene for a new secreted reporter protein
US10612034B2 (en) * 2012-06-01 2020-04-07 Exxonmobil Research And Engineering Company Promoters and terminators for use in eukaryotic cells
US9550998B2 (en) 2012-08-29 2017-01-24 Nature Technology Corporation DNA plasmids with improved expression
US20150322439A1 (en) 2012-11-19 2015-11-12 Nature Technology Corporation Replicative minicircle vectors with improved expression
WO2015143029A1 (fr) 2014-03-18 2015-09-24 The Johns Hopkins University Système de rapporteur génétique moléculaire à base de psma
AU2015236033B2 (en) 2014-03-26 2018-06-07 University Of Maryland, College Park Targeted genome editing in zygotes of domestic large animals
WO2017049132A1 (fr) 2015-09-18 2017-03-23 DNARx Systèmes et méthodes pour l'expression d'acides nucléiques in vivo
EP3600428A4 (fr) 2017-03-23 2020-08-26 Dnarx Systèmes et méthodes pour l'expression d'acides nucléiques in vivo
GB201714330D0 (en) * 2017-09-06 2017-10-18 Järver Peter Oligonucleotides
EP3456821B2 (fr) 2017-09-19 2024-01-24 Deutsches Krebsforschungszentrum Vecteurs d'adn non intégrants destinés à la modification génétique de cellules
WO2019057774A1 (fr) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum Vecteurs d'adn non intégrants pour la modification génétique de cellules
AU2019240068B2 (en) 2018-03-21 2025-03-13 Aldevron, L.L.C. Viral and non-viral nanoplasmid vectors with improved production
CN113943688B (zh) * 2019-07-05 2023-10-20 苏州华赛生物工程技术有限公司 生产胞嘧啶的重组微生物及生产胞嘧啶的方法
WO2021055760A1 (fr) 2019-09-18 2021-03-25 Intergalactic Therapeutics, Inc. Vecteurs d'adn synthétiques et procédés d'utilisation
FR3111913A1 (fr) 2020-06-30 2021-12-31 Eyevensys Construction d’adn pour le traitement de pathologies oculaires
AU2024409925A1 (en) 2023-12-26 2026-08-06 Emmune, Inc. Systems for nucleic acid transfer
WO2025231315A2 (fr) 2024-05-03 2025-11-06 Aldevron, L.L.C. Souches bactériennes hôtes

Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2000014262A2 (fr) * 1998-09-09 2000-03-16 Genzyme Corporation Methylation de plasmides vecteurs
WO2001040478A2 (fr) * 1999-12-06 2001-06-07 Institut Pasteur Polynucleotides isoles a teneur reduite ou renforcee en motifs de regulation epigenetiques et leur utilisation

Family Cites Families (3)

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Publication number Priority date Publication date Assignee Title
FR2738842B1 (fr) † 1995-09-15 1997-10-31 Rhone Poulenc Rorer Sa Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique
US20020065236A1 (en) † 1998-09-09 2002-05-30 Yew Nelson S. CpG reduced plasmids and viral vectors
FR2821855B1 (fr) † 2001-03-09 2004-04-02 Cayla Genes synthetiques et plasmides bacteriens depourvus de cpg

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000014262A2 (fr) * 1998-09-09 2000-03-16 Genzyme Corporation Methylation de plasmides vecteurs
WO2001040478A2 (fr) * 1999-12-06 2001-06-07 Institut Pasteur Polynucleotides isoles a teneur reduite ou renforcee en motifs de regulation epigenetiques et leur utilisation

Non-Patent Citations (8)

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DATABASE EMBL 1 January 1900 (1900-01-01), TIRABY G, ET AL: "NEW SUICIDE GENES AND NEW ASSOCIATIONS OF PYRIMIDINE NUCLEOBASE AND NUCLEOSIDE ANALOGS WITH NEW SUICIDE GENES FOR A GENE THERAPY OF ACQUIRED DISEASES", XP002185408, Database accession no. L37432 *
IGOR LEVCHENKO ET AL.: "Initiator protein pi can bind independently to two domains of the gamma origin core of plasmid R6K: the direct repeats and the A+T-rich segment", NUCLEIC ACIDS RESEARCH, vol. 24, no. 10, 1996, pages 1936 - 1942, XP002185270 *
ISABELLE HENRY ET AL.: "LagoZ et LagZ, deux gènes appauvris en dinucléotides CpG dérivés du gène LacZ pour l'étude des contrôles épigénétiques", C.R. ACAD. SCI. PARIS, vol. 322, 1999, pages 1061 - 1070, XP002185406 *
MARTIN HUG ET AL.: "Transcriptional repression by methylation: cooperativity between a CpG cluster in the promoter and remote CpG-rich regions", FEBS LETTERS, vol. 379, 1996, pages 251 - 254, XP002125021 *
PRITAM SENGUPTA ET AL.: "Methylation in the initiation region of the first exon supresses collagen pro-alpha2(I) gene transcription", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1443, 1998, pages 75 - 89, XP002185407 *
RICHARD S. HALE ET AL.: "Codon optimization of the gene encoding a domain from human type 1 neurofibromin protein results in a threefold improvement in expression level in Escherichia coli", PROTEIN EXPRESSION AND PURIFICATION, vol. 12, March 1998 (1998-03-01), pages 185 - 188, XP001030791 *
THOMAS R. SKOPEK ET AL.: "Synthesis of a lacI gene analogue with reduced CpG content", MUTATION RESEARCH, vol. 349, 1996, pages 163 - 172, XP001041417 *
THOMAS SKOPEK ET AL.: "Effect of target gene CpG content on spontaneous mutation in transgenic mice", MUTATION RESEARCH, vol. 400, 1998, pages 77 - 88, XP001030795 *

Also Published As

Publication number Publication date
EP1366176B1 (fr) 2007-08-15
EP1366176B2 (fr) 2011-10-19
EP1366176A2 (fr) 2003-12-03
AU2002253226B2 (en) 2007-11-15
US20040219677A1 (en) 2004-11-04
CA2440195C (fr) 2011-07-12
ATE370238T1 (de) 2007-09-15
FR2821855B1 (fr) 2004-04-02
DE60221801T3 (de) 2012-05-31
CA2440195A1 (fr) 2002-09-19
DE60221801T2 (de) 2008-05-08
US7244609B2 (en) 2007-07-17
FR2821855A1 (fr) 2002-09-13
DE60221801D1 (de) 2007-09-27
JP4264703B2 (ja) 2009-05-20
WO2002072846A2 (fr) 2002-09-19
WO2002072846A9 (fr) 2003-03-20
JP2004524031A (ja) 2004-08-12

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