BRPI0517380A - molécula de dna, cassete de expressão, célula hospedeira, e, métodos para gerar uma célula hospedeira que expressa um polipeptìdeo de interesse e para produzir um polipeptìdeo de interesse - Google Patents

molécula de dna, cassete de expressão, célula hospedeira, e, métodos para gerar uma célula hospedeira que expressa um polipeptìdeo de interesse e para produzir um polipeptìdeo de interesse

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Publication number
BRPI0517380A
BRPI0517380A BRPI0517380-9A BRPI0517380A BRPI0517380A BR PI0517380 A BRPI0517380 A BR PI0517380A BR PI0517380 A BRPI0517380 A BR PI0517380A BR PI0517380 A BRPI0517380 A BR PI0517380A
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BR
Brazil
Prior art keywords
polypeptide
interest
host cell
dna molecule
methods
Prior art date
Application number
BRPI0517380-9A
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English (en)
Inventor
Arie Pieter Otte
Blokland Henricus Johannes Maria Van
Theodorus Hendrikus Jacobus Kwaks
Richard George Antonius Bernardus Sewalt
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Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Publication of BRPI0517380A publication Critical patent/BRPI0517380A/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Microbiology (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MOLéCULA DE DNA, CASSETE DE EXPRESSãO, CéLULA HOSPEDEIRA, E, MéTODOS PARA GERAR UMA CéLULA HOSPEDEIRA QUE EXPRESSA UM POLIPEPTìDEO DE INTERESSE E PARA PRODUZIR UM POLIPEPTìDEO DE INTERESSE. A invenção proporciona uma molécula de DNA compreendendo uma unidade de transcrição multicistrónica 5 que codifica para i) um polipeptídeo marcador selecionável funcional em uma célula hospedeira eucariótica, e para ii) um polipeptídeo de interesse, sendo que o polipeptídeo de interesse apresenta uma seqúência de partida de tradução separada daquela do polipeptideo marcador selecionável 10, caracterizado pelo fato de que a seqúência codificante para o polipeptídeo de interesse é a jusante da seqúência codificante para o marcador selecionável em referida unidade de transcrição multicistrónica, e a seqúência de ácido nucleico codificando o polipeptídeo marcador selecionável 15 compreende uma mutação que diminui a eficiência de tradução do marcador selecionável em uma célula hospedeira eucariótica. A invenção também proporciona métodos para obter células hospedeiras expressando um polipeptídeo de interesse, sendo que referidas células hospedeiras compreendendo as moléculas de DNA de 20 da invenção. A invenção proporciona adicionalmente a produção de polipeptídeos de interesse, compreendendo cultivar células hospedeiras compreendendo as moléculas de DNA de acordo com a invenção.
BRPI0517380-9A 2004-11-08 2005-11-07 molécula de dna, cassete de expressão, célula hospedeira, e, métodos para gerar uma célula hospedeira que expressa um polipeptìdeo de interesse e para produzir um polipeptìdeo de interesse BRPI0517380A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US62630104P 2004-11-08 2004-11-08
EP04105593 2004-11-08
US69661005P 2005-07-05 2005-07-05
PCT/EP2005/055794 WO2006048459A2 (en) 2004-11-08 2005-11-07 Selection of host cells expressing protein at high levels

Publications (1)

Publication Number Publication Date
BRPI0517380A true BRPI0517380A (pt) 2008-10-07

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BRPI0517380-9A BRPI0517380A (pt) 2004-11-08 2005-11-07 molécula de dna, cassete de expressão, célula hospedeira, e, métodos para gerar uma célula hospedeira que expressa um polipeptìdeo de interesse e para produzir um polipeptìdeo de interesse

Country Status (13)

Country Link
US (1) US20060141577A1 (pt)
EP (1) EP1809750B1 (pt)
JP (1) JP5291341B2 (pt)
KR (1) KR101271884B1 (pt)
AU (1) AU2005300503B2 (pt)
BR (1) BRPI0517380A (pt)
CA (1) CA2581422C (pt)
EA (1) EA010863B1 (pt)
IL (1) IL183035A0 (pt)
MX (1) MX2007004595A (pt)
NO (1) NO344469B1 (pt)
PL (1) PL1809750T3 (pt)
WO (1) WO2006048459A2 (pt)

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US7968700B2 (en) * 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof

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EA200701023A1 (ru) 2007-10-26
KR20070090151A (ko) 2007-09-05
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EP1809750A2 (en) 2007-07-25
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US20060141577A1 (en) 2006-06-29
AU2005300503B2 (en) 2010-12-16
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WO2006048459A3 (en) 2006-08-10
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NO20072845L (no) 2007-06-05
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