WO2003000920A3 - Procedes de caracterisation de molecules d'acide nucleique - Google Patents

Procedes de caracterisation de molecules d'acide nucleique Download PDF

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Publication number
WO2003000920A3
WO2003000920A3 PCT/US2002/019766 US0219766W WO03000920A3 WO 2003000920 A3 WO2003000920 A3 WO 2003000920A3 US 0219766 W US0219766 W US 0219766W WO 03000920 A3 WO03000920 A3 WO 03000920A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
local
acid molecule
modified
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/019766
Other languages
English (en)
Other versions
WO2003000920A9 (fr
WO2003000920A2 (fr
Inventor
Daniel Branton
Hui Wang
Stephen Laderman
Jeffrey Sampson
Zohar Yakhani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agilent Technologies Inc
Harvard University
Original Assignee
Agilent Technologies Inc
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agilent Technologies Inc, Harvard University filed Critical Agilent Technologies Inc
Priority to EP02747942A priority Critical patent/EP1478771A4/fr
Priority to AU2002318386A priority patent/AU2002318386A1/en
Publication of WO2003000920A2 publication Critical patent/WO2003000920A2/fr
Publication of WO2003000920A9 publication Critical patent/WO2003000920A9/fr
Anticipated expiration legal-status Critical
Publication of WO2003000920A3 publication Critical patent/WO2003000920A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N7/00—Television systems
    • H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Multimedia (AREA)
  • Signal Processing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Selon l'invention, une zone locale transversale, une charge locale ou la chimie locale d'une molécule est modifiée en vue de sonder les caractéristiques d'une molécule d'acide nucléique cible ou d'une population de molécules d'acide nucléique cibles. L'acide nucléique modifié est mis en contact avec un substrat comprenant un détecteur répondant à la modification dans la zone locale transversale, dans la charge locale ou dans la chimie locale de la molécule d'acide nucléique. La molécule d'acide nucléique modifiée traverse un volume défini et, de préférence, aux dimensions moléculaires, sur le substrat, de manière que des nucléotides de la molécule d'acide nucléique modifiée interagissent avec le détecteur dans un ordre séquentiel, des données en corrélation avec la zone transversale, la charge locale ou la chimie locale de la molécule d'acide nucléique étant obtenues. La molécule d'acide nucléique peut être modifiée au niveau de plusieurs emplacements situés le long de la longueur de la molécule. L'interaction des nucléotides individuels de la molécule d'acide nucléique avec le détecteur dans un ordre séquentiel permet d'obtenir des informations relatives à l'emplacement et à la composition d'une pluralité de sites modifiés le long d'une molécule unique.
PCT/US2002/019766 2001-06-21 2002-06-21 Procedes de caracterisation de molecules d'acide nucleique Ceased WO2003000920A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02747942A EP1478771A4 (fr) 2001-06-21 2002-06-21 Procedes de caracterisation de molecules d'acide nucleique
AU2002318386A AU2002318386A1 (en) 2001-06-21 2002-06-21 Methods for characterization of nucleic acid molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29987001P 2001-06-21 2001-06-21
US60/299,870 2001-06-21

Publications (3)

Publication Number Publication Date
WO2003000920A2 WO2003000920A2 (fr) 2003-01-03
WO2003000920A9 WO2003000920A9 (fr) 2003-03-20
WO2003000920A3 true WO2003000920A3 (fr) 2004-09-23

Family

ID=23156656

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/019766 Ceased WO2003000920A2 (fr) 2001-06-21 2002-06-21 Procedes de caracterisation de molecules d'acide nucleique

Country Status (3)

Country Link
EP (1) EP1478771A4 (fr)
AU (1) AU2002318386A1 (fr)
WO (1) WO2003000920A2 (fr)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
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US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US7410564B2 (en) * 2003-01-27 2008-08-12 Agilent Technologies, Inc. Apparatus and method for biopolymer identification during translocation through a nanopore
US7282130B2 (en) 2003-01-31 2007-10-16 Agilent Technologies, Inc. Apparatus and method for control of biopolymer translocation through a nanopore
WO2005017025A2 (fr) 2003-08-15 2005-02-24 The President And Fellows Of Harvard College Etude de molecules de polymere et conformations avec un nanopore
WO2006028508A2 (fr) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Procedes et appareil de caracterisation de polynucleotides
GB0713143D0 (en) * 2007-07-06 2007-08-15 Ucl Business Plc Nucleic acid detection method
EP2201136B1 (fr) 2007-10-01 2017-12-06 Nabsys 2.0 LLC Séquençage par nanopore et hybridation de sondes pour former des complexes ternaires et l'alignement de plage variable
US8882980B2 (en) 2008-09-03 2014-11-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
WO2010111605A2 (fr) * 2009-03-27 2010-09-30 Nabsys, Inc. La présente invention concerne des dispositifs et des procédés d'analyse de biomolécules et des sondes liées à celles-ci
EP2507387B1 (fr) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Dispositif et procedee d'analyse biochimique
US9005425B2 (en) 2010-03-05 2015-04-14 University Of Utah Research Foundation Detection of nucleic acid lesions and adducts using nanopores
US8828211B2 (en) 2010-06-08 2014-09-09 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
EP2593568B1 (fr) 2010-07-14 2017-04-19 The Curators Of The University Of Missouri Détection de molécules individuelles d'acides nucléiques facilitée par des nanopores
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
WO2012042226A2 (fr) 2010-10-01 2012-04-05 Oxford Nanopore Technologies Limited Appareil d'analyse biochimique et vanne rotative
WO2012067911A1 (fr) 2010-11-16 2012-05-24 Nabsys, Inc. Procédés de séquençage d'une biomolécule par détection de positions relatives de sondes hybridées
WO2012109574A2 (fr) 2011-02-11 2012-08-16 Nabsys, Inc. Procédés de dosage à l'aide de protéines de liaison à l'adn
WO2012177792A2 (fr) 2011-06-24 2012-12-27 Sequenom, Inc. Méthodes et procédés pour estimation non invasive de variation génétique
GB2498021A (en) * 2011-09-07 2013-07-03 Univ Utah Res Found Detection of Nucleic acid lesions and adducts using nanopores
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2013052913A2 (fr) 2011-10-06 2013-04-11 Sequenom, Inc. Procédés et processus d'évaluation non invasive de variations génétiques
EP2764458B1 (fr) 2011-10-06 2021-04-07 Sequenom, Inc. Méthodes et procédés pour évaluation non invasive de variations génétiques
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
ES2929923T3 (es) 2012-01-20 2022-12-02 Sequenom Inc Procesos de diagnóstico que condicionan las condiciones experimentales
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
EP2956550B1 (fr) 2013-01-18 2020-04-08 Nabsys 2.0 LLC Liaison améliorée d'une sonde
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9732379B2 (en) 2013-03-15 2017-08-15 The Curators Of The University Of Missouri Encoded nanopore sensor for multiplex nucleic acids detection
EP4187543A1 (fr) 2013-04-03 2023-05-31 Sequenom, Inc. Procédés et procédés d'évaluation non invasive de variations génétiques
AU2014268377B2 (en) 2013-05-24 2020-10-08 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
DK3011051T3 (en) 2013-06-21 2019-04-23 Sequenom Inc Method for non-invasive evaluation of genetic variations
US10964409B2 (en) 2013-10-04 2021-03-30 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP6680680B2 (ja) 2013-10-07 2020-04-15 セクエノム， インコーポレイテッド 染色体変化の非侵襲性評価のための方法およびプロセス
US11783911B2 (en) 2014-07-30 2023-10-10 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
US10829804B2 (en) * 2015-03-23 2020-11-10 The University Of North Carolina At Chapel Hill Method for identification and enumeration of nucleic acid sequences, expression, splice variant, translocation, copy, or DNA methylation changes using combined nuclease, ligase, polymerase, terminal transferase, and sequencing reactions
US11200963B2 (en) 2016-07-27 2021-12-14 Sequenom, Inc. Genetic copy number alteration classifications
CA3207879C (fr) 2017-01-24 2026-06-16 Sequenom, Inc. Methodes et procedes d'evaluation de variations genetiques
EP3998350B1 (fr) 2017-03-17 2025-05-07 Sequenom, Inc. Procédés et techniques d'évaluation d'un mosaïcisme génétique

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20030104428A1 (en) * 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules

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US6362002B1 (en) * 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
IL156002A0 (en) * 1997-02-12 2003-12-23 Eugene Y Chan Methods and products for analyzing polymers
JP2003527075A (ja) * 1999-03-25 2003-09-16 ハイセック，インコーポレーテッド ハイブリダイゼーションによる配列分析のための、溶液ベースの方法
EP1192103A1 (fr) * 1999-06-22 2002-04-03 President And Fellows of Harvard College Procede permettant de respecter les caracteristiques dimensionnelles d'une structure a l'etat solide
EP1212462B1 (fr) * 1999-09-07 2006-01-25 The Regents of the University of California Methodes permettant de determiner la presence d'acides nucleiques double brin dans un echantillon
AU2002239284A1 (en) * 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20030104428A1 (en) * 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1478771A4 *

Also Published As

Publication number Publication date
EP1478771A4 (fr) 2005-06-15
EP1478771A2 (fr) 2004-11-24
WO2003000920A9 (fr) 2003-03-20
WO2003000920A2 (fr) 2003-01-03
AU2002318386A1 (en) 2003-01-08

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