WO2003010512A3 - Separation solide-liquide a grande vitesse - Google Patents

Separation solide-liquide a grande vitesse Download PDF

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Publication number
WO2003010512A3
WO2003010512A3 PCT/US2002/023546 US0223546W WO03010512A3 WO 2003010512 A3 WO2003010512 A3 WO 2003010512A3 US 0223546 W US0223546 W US 0223546W WO 03010512 A3 WO03010512 A3 WO 03010512A3
Authority
WO
WIPO (PCT)
Prior art keywords
beads
circulating tank
solid
communicating
loop
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/023546
Other languages
English (en)
Other versions
WO2003010512A2 (fr
Inventor
Bozidar Stipanovic
Edward A Jakush
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ACCURATE POLYMERS Inc
Original Assignee
ACCURATE POLYMERS Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ACCURATE POLYMERS Inc filed Critical ACCURATE POLYMERS Inc
Priority to AU2002355285A priority Critical patent/AU2002355285A1/en
Publication of WO2003010512A2 publication Critical patent/WO2003010512A2/fr
Publication of WO2003010512A3 publication Critical patent/WO2003010512A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)

Abstract

L'invention concerne un procédé et un dispositif pour la séparation et la purification d'affinité. Les étapes sont les suivantes: (a) incubation de perles reconstituées avec un fluide à faibles concentrations de composé cible (charge); (b) introduction des perles 'chargées' dans un réservoir de circulation en amont, relié à un système de séparation solide-liquide aux fins d'alimentation (du type centrifugeuse continue à grande vitesse); le système en question présente deux points d'accès supplémentaires, l'un communiquant vers l'aval pour le transport de solutés hors de la boucle par le liquide surnageant (centrifugat), et l'autre communiquant vers l'amont pour le retour de concentré dans le réservoir de circulation, ce qui permet de maintenir efficacement les perles d'affinité dans la boucle; (c) concentration de la suspension des perles incubées en un volume minimum restant fluide et pompable; (d) dilution du concentré dans le réservoir de circulation avec des volumes de solution tampon fraîche et poursuite de la centrifugation, ce qui permet d'éliminer les sous-produits et les impuretés solubles non liés, dans le liquide surnageant (centrifugat); (e) modification des conditions dans le réservoir de circulation pour libérer efficacement à partir du complexe le composé cible avec les perles d'affinité, puis centrifugation pour séparer le composé cible des perles non chargées, et transport de ces perles hors de la boucle vers l'aval par le liquide surnageant (centrifugat); et (f) lavage des perles d'affinité restant dans la boucle, puis transfert de ces perles vers un réservoir de rétention pour des opérations supplémentaires de nettoyage, de stérilisation et de validation, avant le retour dans la boucle.
PCT/US2002/023546 2001-07-23 2002-07-23 Separation solide-liquide a grande vitesse Ceased WO2003010512A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002355285A AU2002355285A1 (en) 2001-07-23 2002-07-23 High-speed, solid-liquid separation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30742601P 2001-07-23 2001-07-23
US60/307,426 2001-07-23

Publications (2)

Publication Number Publication Date
WO2003010512A2 WO2003010512A2 (fr) 2003-02-06
WO2003010512A3 true WO2003010512A3 (fr) 2003-10-02

Family

ID=23189715

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/023546 Ceased WO2003010512A2 (fr) 2001-07-23 2002-07-23 Separation solide-liquide a grande vitesse

Country Status (2)

Country Link
AU (1) AU2002355285A1 (fr)
WO (1) WO2003010512A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006296399B2 (en) 2005-09-30 2011-01-20 Medimmune Limited Interleukin-13 antibody composition
WO2019183334A1 (fr) 2018-03-21 2019-09-26 Waters Technologies Corporation Préparation d'échantillon à base d'affinité élevée sans anticorps, sorbants, dispositifs et méthodes
CN113069790B (zh) * 2021-03-30 2022-08-30 临沂大学 用于光系统ii内周天线cp43和cp47的提取制备装置及方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4780210A (en) * 1986-01-10 1988-10-25 Her Majesty The Queen In Right Of Canada Tangential flow affinity ultrafiltration
US5567615A (en) * 1993-12-23 1996-10-22 Pall Corporation Affinity separation method
US6315900B1 (en) * 1998-06-03 2001-11-13 Accurate Polymers Static separation method using non-porous cellulose beads

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4780210A (en) * 1986-01-10 1988-10-25 Her Majesty The Queen In Right Of Canada Tangential flow affinity ultrafiltration
US5567615A (en) * 1993-12-23 1996-10-22 Pall Corporation Affinity separation method
US6315900B1 (en) * 1998-06-03 2001-11-13 Accurate Polymers Static separation method using non-porous cellulose beads

Also Published As

Publication number Publication date
WO2003010512A2 (fr) 2003-02-06
AU2002355285A1 (en) 2003-02-17

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