WO2003016524A1 - Procede de production d'alcool a partir d'amidon - Google Patents

Procede de production d'alcool a partir d'amidon Download PDF

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Publication number
WO2003016524A1
WO2003016524A1 PCT/JP2002/001980 JP0201980W WO03016524A1 WO 2003016524 A1 WO2003016524 A1 WO 2003016524A1 JP 0201980 W JP0201980 W JP 0201980W WO 03016524 A1 WO03016524 A1 WO 03016524A1
Authority
WO
WIPO (PCT)
Prior art keywords
yeast
starch
amylase
glucoamylase
surface layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2002/001980
Other languages
English (en)
Japanese (ja)
Inventor
Hideki Fukuda
Akihiko Kondo
Atsuo Tanaka
Mitsuyoshi Ueda
Eiichi Satoh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kansai Chemical Engineering Co Ltd
Original Assignee
Kansai Chemical Engineering Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kansai Chemical Engineering Co Ltd filed Critical Kansai Chemical Engineering Co Ltd
Priority to JP2003521834A priority Critical patent/JP4189317B2/ja
Priority to PCT/JP2002/008234 priority patent/WO2003016525A1/fr
Publication of WO2003016524A1 publication Critical patent/WO2003016524A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On peut obtenir directement et efficacement un alcool à partir d'amidon par fermentation lorsqu'on intègre dans une levure, un plasmide qui est construit de manière à comporter de la glucoamylase sur la couche de surface et de l'alpha-amylase secrète, et qu'on laisse ensuite croître la levure résultante avec un substrat à base d'amidon. Comparativement à une levure présentant de la glucoamylase et de l'alpha-amylase sur la couche de surface de la cellule, une levure présentant de l'alpha-amylase sur la couche de surface de la cellule et sécrétant de la glucoamylase ou une levure présentant de la glucoamylase sur la couche de surface, la levure précédemment décrite présente une excellente capacité de fermentation alcoolique et présente un rendement alcoolique de 40 %, c'est-à-dire, comparable à la fermentation alcoolique à partir de glucose. L'utilisation d'alpha-amylase provenant de Streptococcus bovis permet de produire de l'éthanol directement à partir d'amidon non cuit (amidon natif).
PCT/JP2002/001980 2001-08-16 2002-03-04 Procede de production d'alcool a partir d'amidon Ceased WO2003016524A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2003521834A JP4189317B2 (ja) 2001-08-16 2002-08-12 デンプンからアルコールを製造する方法
PCT/JP2002/008234 WO2003016525A1 (fr) 2001-08-16 2002-08-12 Procede de production d'alcool a partir d'amidon

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001-247134 2001-08-16
JP2001247134 2001-08-16

Publications (1)

Publication Number Publication Date
WO2003016524A1 true WO2003016524A1 (fr) 2003-02-27

Family

ID=19076530

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/001980 Ceased WO2003016524A1 (fr) 2001-08-16 2002-03-04 Procede de production d'alcool a partir d'amidon

Country Status (2)

Country Link
JP (1) JP4189317B2 (fr)
WO (1) WO2003016524A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10253337B2 (en) 2016-12-05 2019-04-09 Stellenbosch University Recombinant yeast and use thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05236942A (ja) * 1992-02-27 1993-09-17 Tsusho Sangyosho Kiso Sangyo Kyokucho 新規凝集性アルコ−ル発酵酵母

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05236942A (ja) * 1992-02-27 1993-09-17 Tsusho Sangyosho Kiso Sangyo Kyokucho 新規凝集性アルコ−ル発酵酵母

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MURAI T. ET AL.: "Evaluation of the function of arming yeast displaying glucoamylase on its cell surface by direct fermentation of corn to ethanol", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 86, no. 6, 1998, pages 569 - 572, XP002942427 *
NAKAMURA Y. ET AL.: "Alcohol fermentation of starch by a genetic recombinant yeast having glucoamylase activity", BIOTECHNOLOGY AND BIOENGINEERING, vol. 53, 1997, pages 21 - 25, XP002952402 *
SATOH E. ET AL.: "Molecular cloning and expression of two alpha-amylase genes from streptococcus bovis 148 in escherichia coli", APPL. ENVIRON. MICROBIOL., vol. 59, no. 11, November 1993 (1993-11-01), pages 3669 - 3673, XP002952401 *
TANAKA A. ET AL.: "Cell surface engineering of yeast: construction of arming yeast with biocatalyst", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 90, no. 2, 2000, pages 125 - 136, XP002947291 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10253337B2 (en) 2016-12-05 2019-04-09 Stellenbosch University Recombinant yeast and use thereof

Also Published As

Publication number Publication date
JP4189317B2 (ja) 2008-12-03
JPWO2003016525A1 (ja) 2005-02-17

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