WO2003018834A3 - Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen - Google Patents

Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen Download PDF

Info

Publication number
WO2003018834A3
WO2003018834A3 PCT/DE2002/003122 DE0203122W WO03018834A3 WO 2003018834 A3 WO2003018834 A3 WO 2003018834A3 DE 0203122 W DE0203122 W DE 0203122W WO 03018834 A3 WO03018834 A3 WO 03018834A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid oligomer
detection
hybridization events
ligands
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2002/003122
Other languages
English (en)
French (fr)
Other versions
WO2003018834A2 (de
Inventor
Gerhard Hartwich
Peter Frischmann
Ute Haker
Herbert Wieder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Friz Biochem Gesellschaft fuer Bioanalytik mbH
Original Assignee
Friz Biochem Gesellschaft fuer Bioanalytik mbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10141691A external-priority patent/DE10141691A1/de
Application filed by Friz Biochem Gesellschaft fuer Bioanalytik mbH filed Critical Friz Biochem Gesellschaft fuer Bioanalytik mbH
Priority to EP02762252A priority Critical patent/EP1554396B1/de
Priority to AT02762252T priority patent/ATE513058T1/de
Priority to US10/487,816 priority patent/US20060228703A1/en
Publication of WO2003018834A2 publication Critical patent/WO2003018834A2/de
Publication of WO2003018834A3 publication Critical patent/WO2003018834A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Beschrieben wird ein Verfahren zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen, das die Schritte Bereitstellen einer modifizierten Oberfläche, wobei die Modifikation in der Anbindung wenigstens einer Art von Ligat-Nukleinsäureoligomeren besteht, Bereitstellen von Signal-Nukleinsäureoligomer-Liganden, Bereitstellen einer Probe mit Nukleinsäureoligomer-Liganden, Inkontaktbringen einer definierten Menge der Signal-Nukleinsäureoligomer-Liganden mit der modifizierten Oberfläche und Inkontaktbringen der Probe und der darin enthaltenen Nukleinsäureoligomer-Liganden mit der modifizierten Oberfläche, Detektion der Signal-Nukleinsäureoligomer-Liganden, sowie Vergleich der bei der Detektion der Signal-Nukleinsäureoligomer-Liganden erhaltenen Werte mit Referenzwerten umfasst.
PCT/DE2002/003122 2001-08-25 2002-08-26 Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen Ceased WO2003018834A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02762252A EP1554396B1 (de) 2001-08-25 2002-08-26 Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen
AT02762252T ATE513058T1 (de) 2001-08-25 2002-08-26 Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen
US10/487,816 US20060228703A1 (en) 2001-08-25 2002-08-26 Displacement assay for the detection of nucleic acid oligomer hybridization events

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10141691A DE10141691A1 (de) 2001-08-25 2001-08-25 Verdrängungsassay zur Detektion von Ligat-Ligand-Assoziationsereignissen
DE10141691.1 2001-08-25
PCT/DE2002/001269 WO2003019194A2 (de) 2001-08-25 2002-04-06 Verdrängungsassay zur detektion von ligat-ligand assoziationsereignissen
DEPCT/DE02/01269 2002-04-06

Publications (2)

Publication Number Publication Date
WO2003018834A2 WO2003018834A2 (de) 2003-03-06
WO2003018834A3 true WO2003018834A3 (de) 2003-09-12

Family

ID=25750293

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/003122 Ceased WO2003018834A2 (de) 2001-08-25 2002-08-26 Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen

Country Status (1)

Country Link
WO (1) WO2003018834A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005031066A (ja) * 2003-04-28 2005-02-03 Canon Inc 固定化核酸プローブの処理方法、核酸の検出方法、核酸濃度分析方法、および核酸検出用キット
DE102007044664B4 (de) 2007-09-18 2012-01-05 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verdrängungsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
DE102008002267B3 (de) 2008-06-06 2009-11-26 Friz Biochem Gesellschaft Für Bioanalytik Mbh Elektrisches Subtrat zum Einsatz als Träger von Biomolekülen
DE102009044795B3 (de) 2009-12-07 2011-04-07 Friz Biochem Gesellschaft Für Bioanalytik Mbh Kompetitionsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
DE102011056606B3 (de) 2011-12-19 2013-01-03 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021530A1 (de) * 1992-04-10 1993-10-28 Messerschmitt-Bölkow-Blohm Gmbh Verfahren und vorrichtung zur empfindlichkeits- und selektivitätssteigerung bei immuno-assays, molekül-rezeptor-, dna-komplementär-dna- und gast-wirtsmolekül-wechselwirkungs-assays
WO1999051778A1 (en) * 1998-04-08 1999-10-14 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
WO1999057319A1 (en) * 1998-05-06 1999-11-11 Clinical Micro Sensors Electronic detection of nucleic acids using monolayers
WO2001007665A2 (en) * 1999-07-26 2001-02-01 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU738189B2 (en) 1996-06-07 2001-09-13 Ge Healthcare (Sv) Corp. Nucleic acid mediated electron transfer
DE19964220C2 (de) 1998-11-23 2003-07-03 Friz Biochem Gmbh Verfahren zur Herstellung einer modifizierten leitfähigen Oberfläche
BR0007571A (pt) 1999-01-18 2001-11-27 Friz Biochem Gmbh OligÈmero de ácido nucléico modificado, processode produção de um oligÈmero de ácido nucléicomodificado, superfìcie condutora modificada,processo de produção de uma superfìciecondutora modificada e processo de detecçãoeletroquìmica de eventos de hibridização deoligÈmero

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021530A1 (de) * 1992-04-10 1993-10-28 Messerschmitt-Bölkow-Blohm Gmbh Verfahren und vorrichtung zur empfindlichkeits- und selektivitätssteigerung bei immuno-assays, molekül-rezeptor-, dna-komplementär-dna- und gast-wirtsmolekül-wechselwirkungs-assays
WO1999051778A1 (en) * 1998-04-08 1999-10-14 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
WO1999057319A1 (en) * 1998-05-06 1999-11-11 Clinical Micro Sensors Electronic detection of nucleic acids using monolayers
WO2001007665A2 (en) * 1999-07-26 2001-02-01 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MARRAZZA G ET AL: "Disposable DNA electrochemical sensor for hybridization detection", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 14, 1999, pages 43 - 51, XP002113746, ISSN: 0956-5663 *
UMEK R M ET AL: "Bioelectronic detection of point mutations using discrimination of the H63D polymorphism of the Hfe gene as a model.", MOLECULAR DIAGNOSIS: A JOURNAL DEVOTED TO THE UNDERSTANDING OF HUMAN DISEASE THROUGH THE CLINICAL APPLICATION OF MOLECULAR BIOLOGY. UNITED STATES DEC 2000, vol. 5, no. 4, December 2000 (2000-12-01), pages 321 - 328, XP008018952, ISSN: 1084-8592 *
WANG J ET AL: "Nucleic-acid immobilization, recognition and detection at chronopotentiometric DNA chips", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 12, no. 7, 1997, pages 587 - 599, XP002113741, ISSN: 0956-5663 *

Also Published As

Publication number Publication date
WO2003018834A2 (de) 2003-03-06

Similar Documents

Publication Publication Date Title
WO2002004113A3 (en) Methods of patterning protein and cell adhesivity
AU2002300939A1 (en) Reagents and Methods for Detecting Analytes, and Devices Comprising Reagents for Detecting Analytes
AU2192801A (en) A method of assessing the amount of nucleic acid in a sample
WO2001077383A3 (en) Nucleic acid detection medium
EP0657737A3 (de) Verfahren und Reagenzien zur Bindung von chemischen Analyten an eine Substratoberfläche, und verwandte analytische Vorrichtungen und diagnostische Methoden.
HK1045334A1 (zh) 缩氨酸受体结紮方法
EP0969282A3 (de) Eine Enzymelektrode und ein Biosensor und ein Messapprat damit
IL134537A0 (en) Nucleic acid encoding an april ligand or a fragment thereof, vectors and host cells containing the same and methods for the production thereof
WO2003060163A8 (en) Discrimination and detection of target nucleotide sequences using mass spectrometry
WO2001044501A3 (de) Verfahren und vorrichtung zum nachweis und zur quantifizierung von biomolekülen
WO2003031977A3 (en) Binding assay using a magnetic field
AU2002360934A1 (en) Method in the form of a dry rapid test for detecting nucleic acids
WO2001057237A3 (en) Methods employing increased dye-fluorescence upon nitroreduction
WO2005010491A3 (en) Method for resupplying reagents in an automatic clinical analyzer
WO2002054072A3 (en) Assay to determine efficacy of treatment for mycobacterial infection
AU2002356632A1 (en) Method for determining nucleic acid analytes
WO2003019194A3 (de) Verdrängungsassay zur detektion von ligat-ligand assoziationsereignissen
AU2462399A (en) Cubilin protein, dna sequences encoding cubilin and uses thereof
WO2003102222A3 (en) Continuous-flow enzyme assay with mass spectrometry detection
CA2407760A1 (en) Methods for diagnosis of low grade astrocytoma
WO2001059683A3 (en) Integrated genomic services
WO2002058533A3 (en) Process for determining target function and identifying drug leads
WO2001096592A3 (en) Reverse detection for identification and/or quantification of nucleotide target sequences on biochips
WO2002089971A3 (de) Hybridverfahren zur herstellung von trägern für die analytbestimmung
WO2004000995A3 (en) Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU BR CA CN IL JP RU US

Kind code of ref document: A2

Designated state(s): AU BR CA CN IL JP RU US ZA

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FR GB GR IE IT LU MC NL PT SE SK TR AU BR CA CN IL JP ZA AT BE BG CH CY CZ DE DK EE ES FR GB GR IE IT LU MC NL PT SE SK

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2002762252

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2002762252

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2006228703

Country of ref document: US

Ref document number: 10487816

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: JP

WWP Wipo information: published in national office

Ref document number: 10487816

Country of ref document: US